Selective responses of three Ginkgo biloba leaf-derived constituents on human intestinal bacteria

The selective responses of Ginkgo biloba leaf-derived materials against six intestinal bacteria was examined using an impregnated paper disk method and compared with that of bilobalide, ginkgolides A and B, kaempferol, and quercetin. The components of G. biloba leaves were characterized as kaempferol 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside), kaempferol 3-O-(2' '-O-beta-D-glucopyranosyl)-alpha-L-rhamnopyranoside, and quercetin 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside) by spectroscopic analysis. The growth responses varied with each bacterial strain tested. At 2 mg/disk, kaempferol 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside) and quercetin 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside) revealed potent inhibition against Clostridium perfringens, and kaempferol 3-O-(2' '-O-beta-D-glucopyranosyl)-alpha-L-rhamnopyranoside showed a clear inhibitory effect on Escherichia coli. At 0.5 mg/disk, quercetin 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside) showed a strong activity against C. perfringens, but weak activity was exhibited by kaempferol 3-O-alpha-(6' "-p-coumaroylglucosyl-beta-1,4-rhamnoside) against C. perfringens and kaempferol 3-O-(2' '-O-beta-D-glucopyranosyl)-alpha-L-rhamnopyranoside against E. coli. No inhibition was observed from treatments conducted with bilobalide, ginkgolides A and B, kaempferol, or quercetin. Furthermore, these isolated compounds did not inhibit Bifidobacterium bifidum, B. longum, B. adolescentis, or Lactobacillus acidophilus.     

Selective growth-inhibiting effects of compounds identified in Tabebuia impetiginosa inner bark on human intestinal bacteria

The growth-inhibiting activity of anthraquinone-2-carboxylic acid and lapachol identified in the inner bark of taheebo, Tabebuia impetiginosa, toward 10 human intestinal bacteria was evaluated by using a paper disk diffusion bioassay and compared to those of seven lapachol congeners (1,4-naphthoquinone, naphthazarin, menadione, lawsone, plumbagin, juglone, and dichlone) as well as two commercially available antibiotics, chloramphenicol and tetracycline. Anthraquinone-2-carboxylic acid exhibited very strong growth inhibition of Clostridium paraputrificum at 1 microg/disk while 100 microg/disk of lapachol was needed for moderate growth inhibition of the same organism. These two isolates exhibited weak inhibition of Clostridium perfringens and Escherichia coli at 100 microg/disk while no adverse effects were observed on the growth of Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Lactobacillus acidophilus, and Lactobacillus casei at 1000 microg/disk. Structure-activity relationships indicate that a methyl group in the C-2 position of 1,4-naphthoquinone derivatives might play an important role in antibacterial activity.     

Degradation of neohesperidin dihydrochalcone by human intestinal bacteria

The degradation of neohesperidin dihydrochalcone by human intestinal microbiota was studied in vitro. Human fecal slurries converted neohesperidin dihydrochalcone anoxically to 3-(3-hydroxy-4-methoxyphenyl)propionic acid or 3-(3,4-dihydroxyphenyl)propionic acid. Two transient intermediates were identified as hesperetin dihydrochalcone 4'-beta-d-glucoside and hesperetin dihydrochalcone. These metabolites suggest that neohesperidin dihydrochalcone is first deglycosylated to hesperetin dihydrochalcone 4'-beta-d-glucoside and subsequently to the aglycon hesperetin dihydrochalcone. The latter is hydrolyzed to the corresponding 3-(3-hydroxy-4-methoxyphenyl)propionic acid and probably phloroglucinol. Eubacterium ramulus and Clostridium orbiscindens were not capable of converting neohesperidin dihydrochalcone. However, hesperetin dihydrochalcone 4'-beta-d-glucoside was converted by E. ramulus to hesperetin dihydrochalcone and further to 3-(3-hydroxy-4-methoxyphenyl)propionic acid, but not by C. orbiscindens. In contrast, hesperetin dihydrochalcone was cleaved to 3-(3-hydroxy-4-methoxyphenyl)propionic acid by both species. The latter reaction was shown to be catalyzed by the phloretin hydrolase from E. ramulus.     

Effect of root exudates on growth of newly isolated nitrifying bacteria from barley rhizoplane

An ammonia-oxidizing bacterium, strain PJA1, and nitrite-oxidizing bacterium, strain PJN1, were newly isolated from the rhizoplane of barley. The cells of strain PJA1 were lobate, compartmentalized, and showed characteristics of the genus Nitrosolobus. The similarity of the 16S rRNA gene to Nitrosolobus multiformis ATCC25196 was 99.04%. The cells of strain PJN1 were rod-shaped. The similarity of the 16S rRNA gene to Nitrobacter agilis ATCC14123 was 98.57%. These newly isolated bacteria were identified as Nitrosolobus sp. PJA1 and Nitrobacter sp. PJN1, respectively. The exudates prepared from barley roots of both the pre-heading and post-heading stages promoted the growth of strain PJA1 (15–20% increase). The growth of the non-rhizoplane strains like Nitrosomonas europaea ATCC25978 was remarkably inhibited by the exudates. The growth of strain PJN1 was also remarkably promoted by the root exudates at the pre-heading stage (6 time increase), and by the root exudates at the post-heading stage (2.5 time increase). The root exudates did not effect the growth of Nitrobacter winogradskyi IFO14297, isolated from the non-rhizoplane.     

Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militaris against Clostridium spp

The growth responses of nine human intestinal bacteria to liquid culture of Cordyceps militaris Link. Pt. (Ascomycotina: Clavicipitaceae) collected from a pupa of Bombyx mori L. (Lepidoptera: Bombycidae) were examined using spectrophotometric and impregnated paper disk methods and compared to those of tetracycline and chloramphenicol, as well as those of Coptis japonica root-derived berberine chloride. The biologically active constituent of the cultures was characterized as cordycepin (3'-deoxyadenosine) by spectroscopic analysis. This compound revealed potent growth-inhibiting activity toward Clostridium paraputrificum and Clostridium perfringens at 10 microgram/disk without adverse effects on the growth of Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium adolescentis, Lactobacillus acidophilus, and Lactobacillus casei, whereas tetracycline and chloramphenicol inhibited the growth of these lactic acid-producing bacteria, clostridia and Escherichia coli. However, C. militaris-derived materials revealed no growth stimulation on the bifidobacteria and lactobacilli. These results may be an indication of at least one of the pharmacological actions of C. militaris. As a naturally occurring antibacterial agent, cordycepin could be useful as a new preventive agent against various diseases caused by clostridia.     

Selection for root colonising bacteria stimulating wheat growth in saline soils

High salinity of soils in arid and semi-arid regions results in desertification and decreased crop yield. One possibility to circumvent this problem is to use root colonising salt tolerant bacterial inoculants which can alleviate salt stress in plants. In the present work, the best five enhanced wheat root tip coloniser bacteria were selected from the rhizosphere of wheat grown in saline soil and were identified by the 16S rRNA gene sequence as Pseudomonas putida, Pseudomonas extremorientalis, Pseudomonas chlororaphis and Pseudomonas aurantiaca. The isolates tolerated salt of 5% NaCl and produced indole acetic acid under saline conditions. Four isolates proved to be very efficient in promoting a significant increase in the shoot, root and dry matter of wheat and were able to survive in saline soil. Four of the isolated strains appeared to be better competitive colonisers than reference strains and probably outcompeted with indigenous microorganisms of the rhizosphere. These results are promising for the application of selected environmentally save microbes in saline agricultural soils.     

Fermentation of plant cell wall derived polysaccharides and their corresponding oligosaccharides by intestinal bacteria

New types of nondigestible oligosaccharides were produced from plant cell wall polysaccharides, and the fermentation of these oligosaccharides and their parental polysaccharides by relevant individual intestinal species of bacteria was studied. Oligosaccharides were produced from soy arabinogalactan, sugar beet arabinan, wheat flour arabinoxylan, polygalacturonan, and rhamnogalacturonan fraction from apple. All of the tested substrates were fermented to some extent by one or more of the individual species of bacteria tested. Bacteroides spp. are able to utilize plant cell wall derived oligosaccharides besides their reported activity toward plant polysaccharides. Bifidobacterium spp. are also able to utilize the rather complex plant cell wall derived oligosaccharides in addition to the bifidogenic fructooligosaccharides. Clostridium spp., Klebsiella spp., and Escherichia coli fermented some of the selected substrates in vitro. These studies do not allow prediction of the fermentation in vivo but give valuable information on the fermentative capability of the tested intestinal strains.     

一株人参根区解磷细菌的筛选、鉴定及对人参生长的影响

人参是我国传统名贵中药材,也是吉林省对外交往中的代名词。吉林参畦土壤有效磷含量低,但大量施用磷肥又会导致人参品质的下降,因此,本研究从人参根区土壤中筛选高效解磷细菌,接种到人参根部,研究其对人参生长、品质、土壤速效养分、土壤酶活性的影响,以期为人参解磷微生物肥料提供优良菌株,从而促进人参产业绿色发展。采用PKO平板初筛及摇瓶复筛从野山参根区土壤筛选高效解磷细菌。盆栽试验以3年生人参种苗为材料,每盆种植6棵单株重相同的人参种苗,设对照(CK)和处理组,待完全展叶后间苗,留取4棵长势一致的人参苗,采用滴灌的方式将菌液接种在人参苗根部附近,生长至红果期(90 d)取样,测定植物生长量和叶绿素含量、土壤速效养分、参根和参叶中NPK的含量、土壤酶活性、参根中微量元素和8种皂苷的含量。结果表明,1)平板初筛和摇瓶复筛得到P1、P7菌株,摇瓶培养中,P1可溶性磷为472.85μg/m L,P7可溶性磷为437.33μg/m L。2)P1处理的人参参根鲜重、干重显著高于P7、BM、CK处理,株高显著高于对照,叶绿素含量显著高于P7、BM、CK(P0.05)。3)P1处理促进人参对土壤钾元素的吸收,提高磷素在参根中的积累量。4)接种菌剂可以不同程度的提高土壤酶活性,以P1处理的效果最显著,P1处理人参根际土壤脲酶、酸性磷酸酶、过氧化物酶活性均为最高。5)P1处理显著提高了人参根中Fe、Mn、Cu的含量,分别较对照增加了52.74%、127.03%、28.96%,P1处理Zn与对照相比增加19.96%;人参根中8种单体皂苷P1处理均显著高于P7、BM、CK。6)经16S rRNA分析结合P1形态初步鉴定为假单胞菌。     

Effects of benlate on leatherleaf fern growth,root morphology and rhizosphere bacteria

Reduced frond production as well as foliar disorders and root damage to leatherleaf fern (Rumohra adiantiformis [Forst.] Ching) coincided with the widespread use of Benlate fungicide in grower operations. These symptoms could not be explained by environmental conditions or the presence of new pathogens. The objective of this study was to examine the effects of Benlate at labeled rates on frond production, root morphology, and soil microbial characteristics of leatherleaf ferns. A separate experiment was conducted by adding Benlate at labeled drench rate to previously untreated soil to evaluate the promotion of soil bacteria. Benlate was applied to leatherleaf fern plants in the greenhouse at labeled foliar and drench rates for 17 months. Fronds were harvested during the last nine months. Roots of the ferns were examined using electron microscopy at termination of the experiment to determine physical and microbial characteristics of the roots. Rhizosphere bacteria were evaluated to determine counts and types. Lettuce seedling bioassays were conducted to evaluate bacterial isolates for possible deleterious effects on plants. Over the nine‐month harvest period, 181 fronds were harvested per pot from the control treatments, 162 for the Benlate foliar treatment, and 113 for the drench treatment. The Benlate drench rate resulted in significant reduction of frond production. However, the Benlate foliar rate was not significantly different from the control. Benlate application resulted in a reduction of root hairs and disruption of the root surfaces, especially at the drench rate. Total bacterial counts were not significantly different among Benlate treatments; however, the biodiversity of these populations was reduced with 46, 36, and 22 individual isolates identified for control, foliar, and drench rate treatments, respectively. Using a lettuce bioassay, compared to seedlings grown on sterile medium, the average seedling growth in the presence of isolates from the control treatment was 83%, 73% from the foliar rate treatment, and 60% from the drench rate treatment. The predominant deleterious isolates include Pseudomonas fluorescens, Ps. putida, and Ps. cepacia. In a laboratory experiment, these bacteria were shown to be promoted within the soil by Benlate application. These results indicate that Benlate has the potential to reduce yields of leatherleaf ferns. This reduction may be caused by direct action of the chemicals within the Benlate formulations or by the promotion of deleterious bacteria within the growing media.     

Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria.

The growth-inhibiting activity of Coptis japonica (Makino) root-derived materials toward eight human intestinal bacteria was examined using an impregnated paper disk method and compared to that of four commercially available isoquinoline alkaloids [berberine sulfate (BS), berberine iodide (BI), palmatine chloride (PC), and palmatine sulfate(PS)], as well as that of Thea sinensis leaf-derived epigallocatechin gallate (EGCG). The biologically active constituents of the Coptis extract were characterized as the isoquinoline alkaloids berberine chloride (BC), palmatine iodide (PI), and coptisine chloride (CC) by spectral analysis. The growth responses varied with both chemical and bacterial strain used. In a test using 500 microg/disk, BC and PI produced a clear inhibitory effect against Bifidobacterium longum, Bifidobacterium bifidum, Clostridium perfringens, and Clostridium paraputrificum, whereas weak or no inhibition was observed in Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactobacillus casei, and Escherichia coli. At 1000 microg/ disk, CC revealed weak or no growth inhibition toward all test bacteria, whereas EGCG exhibited weak growth inhibition against only C. perfringens and C. paraputrificum. Among various isoquinoline alkaloids, BC exhibited more potent inhibitory activity toward C. perfringens than BI and BS, whereas the inhibitory effect was more pronounced in PI compared to PC and PS. The Coptis root-derived materials did not promote growth of B. longum and C. perfringens.     

Effect of dextransucrase cellobiose acceptor products on the growth of human gut bacteria

The selective fermentation by human gut bacteria of gluco-oligosaccharides obtained from the reaction between the glucosyl group of sucrose and cellobiose, catalyzed by dextransucrases (DSR) from Leuconostoc mesenteroides , has been evaluated. Oligosaccharides were fractionated according to their molecular weight, and their effect on the growth of different bacterial groups was studied. To determine the structure (position and configuration of glycosidic linkages)-function relationship, their properties were compared to those of DSR maltose acceptor products (DSRMal) and of recognized prebiotic carbohydrates (fructo-oligosaccharides, FOS). Cellobiose acceptor products (DSRCel) showed bifidogenic properties similar to those of FOS. However, no significant differences related to molecular weight or isomeric configurations were found for DSRCel and DSRMal products.     

大豆根系分泌物中氨基酸对根腐病菌生长的影响

采用砂培和室内模拟方法,研究了两个抗病性不同的大豆品种水溶性根系分泌物中氨基酸组分随作物生长的变化;同时检测了培养基中添加大豆根系分泌物和纯品氨基酸对大豆根腐病菌菌落生长的影响。结果表明,添加大豆苗期和花荚期根分泌物均显著促进尖镰孢菌菌丝生长,添加成熟期根分泌物显著促进腐皮镰孢菌菌丝生长。易感根腐病大豆品种合丰25号花荚期以后根分泌物中氨基酸种类多于抗根腐病大豆品种绥农10号。感病大豆品种根系分泌的氨基酸总量随生育时期增加,在鼓粒期达到最高;抗病大豆品种根系分泌的氨基酸总量在花荚期最高。感病大豆品种根系分泌的主要氨基酸为精氨酸,抗病大豆品种根系分泌的氨基酸主要为天冬氨酸。氨基酸纯品培养中,添加精氨酸和酪氨酸处理的尖镰孢菌菌落直径显著高于不加氨基酸的对照菌落直径;添加丝氨酸和天冬氨酸的处理菌落直径则显著低于对照处理。同时,添加天冬氨酸的培养基上腐皮镰孢菌菌落直径显著低于不加氨基酸的对照。可见,不同大豆品种根系分泌物中氨基酸组分对病原菌生长起着一定的作用,其表现的作用受根际氨基酸种类和氨基酸浓度影响较大,对于不同病原菌的作用存在差异。     

酸性土壤中解磷菌的分离及其促生效果研究

酸性土壤中磷易被固定,磷的生物有效性极低。解磷菌对土壤中难溶性磷具有重要的增溶作用。虽然已有不少解磷菌方面的研究,但是主要集中于中性和石灰性土壤中钙磷的解磷菌报道,而关于酸性土壤中高效溶解铝磷的微生物报道较少。采用培养基和土培试验,首先对酸性土壤上不同植物（胡枝子、大豆、水稻）根际土壤中的解磷菌进行了分离,然后比较了它们对不同磷源（磷酸钙和磷酸铝）的溶解能力,最后研究了它们对大豆生长和磷吸收的影响。通过使用难溶性磷源（磷酸钙和磷酸铝）的固体培养基,分离得到5株优势菌株L1、S1、S2、R1和R2,经16S rRNA序列鉴定,L1属于阮杆菌属（Nguyenibacter）,S1和S2分别属于假单胞菌属（Pseudomonas）和沙雷氏菌属（Serratia）,R1和R2分别属于伯克氏菌属（Burkholderia）和雷尔氏菌属（Ralstonia）。菌株S1、S2、R1和R2对难溶性磷酸钙有较强的溶解能力,对磷酸铝的溶解能力较弱;菌株L1对磷酸铝表现出较高的溶解能力,对难溶性磷酸钙的溶解能力弱。联合接种菌株L1+S1对大豆生长和磷吸收表现出良好的促进效果,而单独接种L1和S1效果不显著。...     

Selective growth inhibition of human leukemia and human lymphoblastoid cells by resveratrol via cell cycle arrest and apoptosis induction

There is great interest in the potential chemopreventive activity of resveratrol against human cancers. However, there are conflicting results on its growth inhibitory effect on normal cells. This project examined the differential effect of resveratrol at physiologically relevant concentrations on nonmalignant (WIL2-NS) and malignant (HL-60) cell lines and compared the underlying mechanisms via cell cycle modulation, apoptosis induction, and genotoxicity potential. Twenty-four hours of exposure to resveratrol was toxic to WIL2-NS and HL-60 cells in a dose-dependent manner. WIL2-NS cells regrew 5 times more than HL-60 cells by 120 h after the removal of 100 microM resveratrol (p < 0.05). Furthermore, significant alterations in cell cycle kinetics were induced by resveratrol in HL-60 cells, but were to a lesser extent for WIL2-NS cells. The proportion of apoptosis was also 3 times higher in HL-60 cells as compared to WIL2-NS cells for 100 microM resveratrol (p < 0.05). In conclusion, resveratrol preferentially inhibited the growth of HL-60 cells via cell cycle modulation and apoptosis induction and subsequently directed the cells to irreversible cell death, whereas the effect on WIL2-NS cells was largely reversible.     

Transcriptome analysis of the duodenum in Wistar rats fed a trypsin inhibitor derived from squid viscera

To investigate the effects of oral administration of a trypsin inhibitor (TI), normal Wistar rats were fed a TI derived from squid (Todarodes pacificus) for 10 weeks and gene expression profiles in the duodenum, pancreas, liver, and muscle were then analyzed using DNA microarrays. Although no significant changes could be observed in growth, food intake, tissue weight, or blood tests among the tissues tested, the duodenum showed the most remarkable changes in the global gene expression profile. Significant up-regulation of mRNAs encoding gastrin, gastrokine, cholecystokinin and somatostatin in the duodenum was validated by qPCR analysis. In gene ontology (GO) analysis of the up-regulated differentially expressed genes (DEGs), GO terms related to keratinization and innate mucosal defense were enriched (p < 0.001) in the category of biological processes in addition to assumable terms such as regulation of secretion and response to nutrients, vesicle-mediated transport, and so forth. In the same analysis, calcium ion binding was listed at the deepest hierarchy in the category of molecular function. These results indicate that the duodenum responds to TI treatment by a wider range of physiological processes than previously assumed such as keratinocyte differentiation and innate mucosal defense, in which calcium plays a crucial role.     

Fresh-weight measurements of roots provide inaccurate estimates of the effects of plant growth-promoting bacteria on root growth: a critical examination

Four strains of plant growth-promoting bacteria (PGPB), including three strains of Azospirillum and Pseudomonas fluorescens 313 were used to inoculate seeds of wheat, tomato, pepper, and cotton. Inoculated seedlings were grown to the two or three-leaf stage. After harvest, seven different environmental and technical conditions were evaluated to determine the effect of these conditions on the reproducibility of fresh and dry root weight measurements. Dry root weight of each sample showed no significant variations (smaller than 1%), despite the variations in measurement conditions. Root fresh weights varied greatly (usually in the range of 4-10%, but up to 18%), and were significantly affected (P≤0.05) by air temperature and relative humidity, air currents, different light intensities during extraction of plants from the substrate, duration of the extraction from soil (depending on the size of the experiment and type of plant growth substrate), and the type of absorbing paper used to blot excess water from harvested roots. Measurements by different technicians did not influence fresh or dry weight values. We conclude that fresh weight determinations are altered by factors independent of the intended experimental variables and should not be used to evaluate the effect of PGPB on plants.     

Absorption of anthocyanins from blueberry extracts by caco-2 human intestinal cell monolayers

Recent studies have shown that dietary polyphenols may contribute to the prevention of cardiovascular disease and cancer. Anthocyanins from different plant sources including blueberries have been shown to possess potential anticancer activities. One of the key factors needed to correctly relate the in vitro study results to human disease outcomes is information about bioavailability. The objectives of the current study were to evaluate the absorption of blueberry anthocyanin extracts using Caco-2 human intestinal cell monolayers and investigate the effects of different aglycones, sugar moieties, and chemical structure on bioavailability of different types of anthocyanins. The results of this study showed that anthocyanins from blueberries could be transported through the Caco-2 cell monolayers although the transport/absorption efficiency was relatively low compared to other aglycone polyphenols. The transport efficiency of anthocyanins averaged approximately 3-4% [less than 1% in delphinidin glucoside (Dp-glc)]. No significant difference in transport/absorption efficiency was observed among three blueberry cultivars. The observed trends among different anthocyanins generally agreed well with some published in vivo results. Dp-glc showed the lowest transport/absorption efficiency, and malvidin glucoside (Mv-glc) showed the highest transport/absorption efficiency. Our result indicates that more free hydroxyl groups and less OCH(3) groups can decrease the bioavailability of anthocyanins. In addition, cyanindin glucoside (Cy-glc) showed significantly higher transport efficiency than cyanidin galactoside (Cy-gal), and peonidin glucoside (Pn-glc) showed significantly higher transport efficiency than peonidin galactoside (Pn-gal), indicating that glucose-based anthocyanins have higher bioavailability than galactose-based anthocyanins.     

A novel method for separating root‐derived organic compounds from root respiration in non‐sterilized soils

A novel method of separating exudates from root respiration in non‐sterilized soils has been developed. The method is based on a simultaneous elution of exudates from rhizosphere and the blowout of CO₂ originating from root respiration. The innovation of the method lies in the function of a membrane pump to drive the movement of air and simultaneously the circulation of water according to the Siphon principle. The separation method was tested by means of ¹⁴C pulse labeling of Lolium perenne to track the C dynamics in the production of rhizosphere CO₂ and of exudates, which were eluted. The total ¹⁴C activity of rhizosphere CO₂ and of eluted exudates was found to be 8.5 % and 2.3 % of total assimilated ¹⁴C, respectively. Thus, at least 19 % of root‐derived C can be accounted to root exudation. However, the suggested Siphon method underestimates the amount of exudates and shows only a minimum of organic substances exuded by roots. The diurnal dynamics of exudation was detected, but no significant day‐night changes were measured in root and microbial respiration. Tight coupling of assimilation with exudation, but not with root and microbial respiration, was observed. The advantages, shortcomings, and possible applications of the Siphon method are discussed.     

作物生长模型与定量遥感参数结合研究进展与展望

作物生长模型与定量遥感参数的结合,不仅满足前者实现区域应用的需求,也有助于提高后者的反演精度,在生态、农业、资源调查与全球气候变化等研究上意义重大。该文从作物生长模型空间应用拓展的角度,对国内外主流作物生长模型、定量遥感参数以及两者结合的参数与方法进行了概述,分析了典型作物生长模型的主要模拟过程及其驱动、初始化、输出等参数,总结了当前定量遥感正反演结果可为作物生长模型区域应用提供的参数数据;建立了作物生长模型模拟过程与定量遥感参数的对应关系,对比分析了作物生长模型与定量遥感参数的不同结合方式。基于以上内容,对作物生长模型面应用的限制因素及其与定量遥感参数的关系、作物生长模型面应用时参数尺度效应的影响、作物生长模型与定量遥感参数耦合方法的发展3个方面展开了讨论,以期为作物生长模型与定量遥感参数开展更好的结合研究提供参考。