Effect of rice straw application on stable carbon isotopes,methanogenic pathway,and fraction of CH4 oxidized in a continuously flooded rice field in winter season

Straw application is a common practice in rice agriculture, but its effect on stable carbon isotopes (δ¹³C) in each process of CH₄ emission, relative contribution of acetate to CH₄ production (F_ac), and fraction of the CH₄ that is oxidized (F_ox) in the winter season is not well known. We investigated CH₄ production and oxidation potentials in paddy soil, CH₄ concentrations in soil pore water and floodwater, and CH₄ emission as well as their δ¹³C from a continuously flooded paddy field with rice straw application (RSA) during the 2007 and 2008 winter seasons and quantified the F_ac and F_ox using the isotopic data. RSA significantly increased CH₄ emission (p < 0.05) but made δ¹³CH_{4 (emission)} lower by 5–8‰. RSA obviously increased CH₄ concentrations in soil pore water and floodwater, but it played a slight role in porewater δ¹³CH₄ while caused floodwater δ¹³CH₄ 4‰ lower. A relatively low contribution of acetate-dependent methanogenesis (about 50%) was observed, and RSA slightly increased F_ac (about 0–10%) at a relatively low temperature whereas greatly decreased it (about 10–20%) at a high temperature. Floodwater CH₄ was much more ¹³C-enriched than porewater CH₄ (p < 0.05), suggesting that about 60–70% of the CH₄ was oxidized at the soil–water interface. F_ox generally decreased in the winter season and it was reduced by RSA (about 10%). Soil temperature was positively correlated with CH₄ flux and CH₄ flux was negatively related to δ¹³CH_{4 (emission)}, suggesting that temperature might be an important factor influencing F_ox, thus CH₄ emission and δ¹³CH_{4 (emission)}. The findings suggest that RSA significantly increased CH₄ production while little affected CH₄ oxidation, thus decreasing F_ox. Moreover, the effect of RSA on methanogenic pathway might be controlled by soil temperature, and RSA generally reduced F_ac and F_ox was the main reason for the emitted CH₄ depleted in ¹³C.     

间隙灌溉和控释肥施用对稻田土壤产甲烷微生物的影响

间隙灌溉和控释肥施用影响稻田CH_4的产生和排放,然而其微生物机理尚不清楚。本研究通过采集稻季田间原位试验新鲜土样,采用核酸定量技术(qPCR)和末端限制性片段长度多态性(T-RFLP)技术,研究间隙灌溉和控释肥施用对稻田土壤产甲烷微生物群落丰度和结构的影响。结果表明,稻季CH_4排放量与古菌、产甲烷菌(mcr A基因)和甲烷氧化菌(pmo A基因)数量均呈极显著正相关关系(P0.01),而与细菌数量无显著相关性。间隙灌溉显著影响产甲烷菌和甲烷氧化菌数量的季节变化,其中烤田抑制产甲烷菌生长,而对甲烷氧化菌数量没有显著影响。与尿素相比,施用控释肥增加了稻田土壤细菌、古菌和产甲烷菌数量,降低了甲烷氧化菌数量。土壤古菌群落的优势T-RFs片段为184bp和391bp,其中184bp片段的相对丰度随着间隙灌溉的进行由45%~55%降低到23%~30%;而391bp片段则相反,其相对丰度由12%~18%增加到23%~26%。典型相关性分析(CCA)表明间隙灌溉显著影响土壤古菌群落结构(P0.001),而控释肥施用对土壤古菌群落结构没有明显影响。     

水稻根际和周围土壤中微生物功能基因丰度与碳、氮状况及其通量之间的关系

Rapid nitrogen（N） transformations and losses occur in the rice rhizosphere through root uptake and microbial activities. However,the relationships between rice roots and rhizosphere microbes for N utilization are still unclear. We analyzed different N forms（NH＋4,NO-3, and dissolved organic N）, microbial biomass N and C, dissolved organic C, CH4 and N2O emissions, and abundance of microbial functional genes in both rhizosphere and bulk soils after 37-d rice growth in a greenhouse pot experiment. Results showed that the dissolved organic C was significantly higher in the rhizosphere soil than in the non-rhizosphere bulk soil, but microbial biomass C showed no significant difference. The concentrations of NH＋4, dissolved organic N, and microbial biomass N in the rhizosphere soil were significantly lower than those of the bulk soil, whereas NO-3in the rhizosphere soil was comparable to that in the bulk soil. The CH4 and N2O fluxes from the rhizosphere soil were much higher than those from the bulk soil. Real-time polymerase chain reaction analysis showed that the abundance of seven selected genes, bacterial and archaeal 16 S rRNA genes, amoA genes of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, nosZ gene, mcrA gene, and pmoA gene, was lower in the rhizosphere soil than in the bulk soil, which is contrary to the results of previous studies. The lower concentration of N in the rhizosphere soil indicated that the competition for N in the rhizosphere soil was very strong, thus having a negative effect on the numbers of microbes. We concluded that when N was limiting, the growth of rhizosphere microorganisms depended on their competitive abilities with rice roots for N.     

中国亚热带中部三峡库区不同土地利用方式下甲烷氧化潜力研究

To compare the CH4 oxidation potential among diferent land uses and seasons,and to observe its response to monsoon precipitation pattern and carbon and nitrogen parameters,a one-year study was conducted for diferent land uses (vegetable field,tilled and non-tilled orchard,upland crops and pine forest) in central subtropical China.Results showed significant diferences in CH4 oxidation potential among diferent land uses(ranging from 3.08 to 0.36 kg CH4 ha-1 year-1).Upland with corn-peanut-sweet potato rotation showed the highest CH4 emission,while pine forest showed the highest CH4 oxidation potential among all land uses.Non-tilled citrus orchard (0.72±0.08 kg CH4 ha-1 year-1)absorbed two times more CH4 than tilled citrus orchard(0.38±0.06kg CH4 ha-1 year-1).Irrespective of diferent vegetation,inorganic N fertilizer application significantly influenced CH4 fluxes across the sites (R2=0.86,P=0.002).Water-filled pore space,soil microbial biomass carbon,and dissolved nitrogen showed significant efects across diferent land uses (31% to 38% of variability)in one linear regression model.However,their cumulative interaction was significant for pine forest only,which might be attributed to undisturbed microbial communities legitimately responding to other variables,leading to net CH4 oxidation in the soil.These results suggested that i)natural soil condition tended to create win-win situation for CH4 oxidation,and agricultural activities could disrupt the oxidation potentials of the soils;and ii)specific management practices including but not limiting to efficient fertilizer application and utilization,water use efciency,and less soil disruption might be required to increase the CH4 uptake from the soil.     

Abundance of transcripts of functional gene reflects the inverse relationship between CH<Subscript>4</Subscript> and N<Subscript>2</Subscript>O emissions during mid-season drainage in acidic paddy soil

Agricultural management significantly affects methane (CH₄) and nitrous oxide (N₂O) emissions from paddy fields. However, little is known about the underlying microbiological mechanism. Field experiment was conducted to investigate the effect of the water regime and straw incorporation on CH₄ and N₂O emissions and soil properties. Quantitative PCR was applied to measure the abundance of soil methanogens, methane-oxidising bacteria, nitrifiers, and denitrifiers according to DNA and mRNA expression levels of microbial genes, including mcrA, pmoA, amoA, and nirK/nirS/nosZ. Field trials showed that the CH₄ and N₂O flux rates were negatively correlated with each other, and N₂O emissions were far lower than CH₄ emissions. Drainage and straw incorporation affected functional gene abundance through altered soil environment. The present (DNA-level) gene abundances of amoA, nosZ, and mcrA were higher with straw incorporation than those without straw incorporation, and they were positively correlated with high concentrations of soil exchangeable NH₄⁺ and dissolved organic carbon. The active (mRNA-level) gene abundance of mcrA was lower in the drainage treatment than in continuous flooding, which was negatively correlated with soil redox potential (Eh). The CH₄ flux rate was significantly and positively correlated with active mcrA abundance but negatively correlated with Eh. The N₂O flux rate was significantly and positively correlated with present and active nirS abundance and positively correlated with soil Eh. Thus, we demonstrated that active gene abundance, such as of mcrA for CH₄ and nirS for N₂O, reflects the contradictory relationship between CH₄ and N₂O emissions regulated by soil Eh in acidic paddy soils.     

Stimulation of methane consumption by endogenous CH4 production in aerobic grassland soil

The net CH₄ flux in terrestrial ecosystems is mostly the result of simultaneous gross CH₄ production (P₄CH) and gross CH₄ consumption (C₄CH) rates. However, it is not well-understood how endogenous P₄CH may influence C₄CH and hence the net CH₄ flux outcome in upland soils. Gross CH₄ consumption may be either stimulated or outweighed by P₄CH, resulting in higher or lower net CH₄ uptake rates, respectively. To investigate gross CH₄ fluxes, we incubated intact soil cores taken from a permanent grassland with and without difluoromethane (DFM; blocks CH₄ oxidation). The gross CH₄ consumption was calculated by taking the DFM-measured rate of P₄CH into account. Most of the oxic cores exhibited a more than 30 times higher C₄CH than P₄CH rate. However, four cores showed a considerably higher gross CH₄ production, accompanied by a stimulated gross CH₄ uptake which was entirely masked in the net CH₄ flux (C₄CH/P₄CH ratios of 0.9-2.3). In three of the four cores macro-fauna (earthworms and Scarab beetle larvae) were present. Removing the animals and homogenizing the intact core structure lowered P₄CH by 27-100%. Subsequent incubation of Scarabidae larvae such as Pachnoda sp., Cetonia sp. and others revealed animal CH₄ and N₂O production rates within the range of those observed in the inhabited cores. Moreover, in-situ soil air CH₄ concentrations obtained from four depth profiles (0-50 cm depth) at the grassland site also indicated that temporarily, small-scale local hot spots of CH₄ and N₂O production existed within otherwise aerobic soil profiles. These spots indicated a stimulated CH₄ uptake after the CH₄ source (of whatever nature) had ceased. The results suggest that soil macro-fauna, anaerobic soil microsites or both combined may provide suitable conditions for CH₄ production in otherwise oxic soil environments. Hence, endogenous CH₄ production in upland soil may partially be related to the density and species composition of soil invertebrates, with a potential impact on the CH₄ sink capacity of these soils.     

Diurnal and seasonal variations in CH4 flux from termite mounds in tropical savannas of the Northern Territory, Australia

Termites are estimated to contribute between <5 and 19% of the global methane (CH₄) emissions. These estimates have large uncertainties because of the limited number of field-based studies and species studied, as well as issues of diurnal and seasonal variations. We measured CH₄ fluxes from four common mound-building termite species (Microcerotermes nervosus, M. serratus, Tumulitermes pastinator and Amitermes darwini) diurnally and seasonally in tropical savannas in the Northern Territory, Australia. Our results showed that there were significant diel and seasonal variations of CH₄ emissions from termite mounds and we observed large species specific differences. On a diurnal basis, CH₄ fluxes were least at the coolest time of the day (∼07.00 h) and greatest at the warmest (∼15.00 h) for all species for both wet and dry seasons. We observed a strong and significant positive correlation between CH₄ flux and mound temperature for all species. A mound excavation experiment demonstrated that the positive temperature effect on CH₄ emissions was not related to termite movement in and out of a mound but probably a direct effect of temperature on methanogenesis in the termite gut. Fluxes in the wet season were 5-26-fold greater than those in the dry season. A multiple stepwise regression model including mound temperature and mound water content described 70-99% of the seasonal variations in CH₄ fluxes for different species. CH₄ fluxes from M. nervosus, which was the most abundant mound-building termite species at our sites, had significantly lower fluxes than the other three species measured. Our data demonstrate that CH₄ flux estimates could result in large under- or over-estimation of CH₄ emissions from termites if the diurnal, seasonal and species specific variations are not accounted for, especially when flux data are extrapolated to landscape scales.     

Increased moisture and methanogenesis contribute to reduced methane oxidation in elevated CO2 soils

Awareness of global warming has stimulated research on environmental controls of soil methane (CH₄) consumption and the effects of increasing atmospheric carbon dioxide (CO₂) on the terrestrial CH₄ sink. In this study, factors impacting soil CH₄ consumption were investigated using laboratory incubations of soils collected at the Free Air Carbon Transfer and Storage I site in the Duke Forest, NC, where plots have been exposed to ambient (370 μL L⁻¹) or elevated (ambient + 200 μL L⁻¹) CO₂ since August 1996. Over 1 year, nearly 90% of the 360 incubations showed net CH₄ consumption, confirming that CH₄-oxidizing (methanotrophic) bacteria were active. Soil moisture was significantly (p < 0.01) higher in the 25–30 cm layer of elevated CO₂ soils over the length of the study, but soil moisture was equal between CO₂ treatments in shallower soils. The increased soil moisture corresponded to decreased net CH₄ oxidation, as elevated CO₂ soils also oxidized 70% less CH₄ at the 25–30 cm depth compared to ambient CO₂ soils, while CH₄ consumption was equal between treatments in shallower soils. Soil moisture content predicted (p < 0.05) CH₄ consumption in upper layers of ambient CO₂ soils, but this relationship was not significant in elevated CO₂ soils at any depth, suggesting that environmental factors in addition to moisture were influencing net CH₄ oxidation under elevated CO₂. More than 6% of the activity assays showed net CH₄ production, and of these, 80% contained soils from elevated CO₂ plots. In addition, more than 50% of the CH₄-producing flasks from elevated CO₂ sites contained deeper (25–30 cm) soils. These results indicate that subsurface (25 cm+) CH₄ production contributes to decreased net CH₄ consumption under elevated CO₂ in otherwise aerobic soils.     

Methane emissions from stems of Fraxinus mandshurica var. japonica trees in a floodplain forest

We measured methane (CH₄) emissions from the stem surfaces of mature Fraxinus mandshurica var. japonica trees in a floodplain forest. Flux measurements were conducted almost monthly from May to October 2005, and positive CH₄ fluxes were detected throughout the study period, including the leafless season. The mean CH₄ flux was 176 and 97 μg CH₄ m⁻² h⁻¹ at the lower (15 cm above the ground) and upper (70 cm above the ground) stem positions, respectively. The CH₄ concentration was lower in soil gas than in ambient air to a depth of at least 40 cm. One possible source of CH₄ emitted from the stems might be the dissolved CH₄ in groundwater; maximum concentrations were 10,000 times higher than atmospheric CH₄ concentrations. Our results suggest that CH₄ transport from the submerged soil layer to the atmosphere may occur through internal air spaces in tree bodies.     

秸秆条带状覆盖对稻田CH_4和N_2O排放的影响

采用3种秸秆还田方式(对照、秸秆均匀混施和秸秆条带状覆盖)进行田间试验,观测稻田CH4和N2O的排放通量,以探讨秸秆条带状覆盖对稻田CH4和N2O排放的影响。结果表明:秸秆条带状覆盖的CH4排放量是对照的2.7倍,二者的N2O排放量无明显差异;秸秆条带状覆盖的稻田CH4排放量较秸秆均匀混施减少32%,其N2O排放量是后者的5.1倍;稻田排放CH4和N2O的全球增温潜势(GWP)为:秸秆均匀混施秸秆条带状覆盖对照,且差异显著;秸秆条带状覆盖的水稻产量分别较对照和秸秆均匀混施增加27%和17%。秸秆条带状覆盖是值得推荐的稻季秸秆还田方式。     

不同施肥处理稻田甲烷和氧化亚氮排放特征

采用静态箱－气相色谱法对长期不同施肥处理(NPKS、CK、NPK和NKM)的稻田CH₄和N₂O排放进行了观测。结果表明,稻田CH₄和N₂O排放季节变化规律明显不同,二者排放通量季节变化呈显著负相关(p<0.01)。与单施化肥和CK相比,施用有机肥显著促进CH₄排放,排放量最高的NPKS处理早晚稻田排放量分别是：526.68 kg/hm²和1072.92 kg/hm²。对于N₂O排放,早稻田各处理间差异不显著,NPK处理排放量最大,为1.48 kg/hm²;晚稻田各处理差异极显著(p<0.01),NPKS处理排放量最大,为1.40 kg/hm²。晚稻田CH₄排放通量和10 cm土层温度及土壤pH值相关极显著(p<0.01),并与二者存在显著的指数关系。没发现N₂O排放通量与温度及pH值间存在显著相关。稻田CH₄和N₂O排放受多种因素影响,但对全球变暖的贡献率CH₄远大于N₂O。NPKS处理的增温潜势最大,NPK处理的最小。     

Methane flux characteristics in forest soils under an East Asian monsoon climate

Methane (CH₄) uptake by soil can possibly be suppressed more in regions with heavy summer precipitation, such as those under the East Asian monsoon climate, as compared to that in regions with a dry summer. In order to determine how precipitation patterns affect seasonal and spatial variations in CH₄ fluxes in temperate forest soils, such fluxes and selected environmental variables were measured on different parts of a hill slope in a cypress forest in central Japan. On the upper and middle parts of the slope, CH₄ uptake was observed throughout the year, and the uptake rates increased slightly with soil temperature and decreased with soil water content. The CH₄ flux predicted using data for the middle and upper parts of the slope ranged from −1.12 to −0.83 kg-CH₄ ha⁻¹ y⁻¹ (i.e. CH₄ uptake by soil) and from −2.30 to −2.04 kg-CH₄ ha⁻¹ y⁻¹, respectively. In contrast, in the relatively wet lower part of the slope near an in-stream wetland, large CH₄ emissions (>2 mg-CH₄ m⁻¹ d⁻¹) were observed during the rainy summer. In this wetter plot, the soil functioned as a net annual CH₄ source in a rainy year. Hence the variation in CH₄ flux with a change in soil water conditions and soil temperature on the lower part of the slope contrasted to that on the upper and middle parts of the slope. The predicted CH₄ flux for this lower plot ranged from −0.45 kg-CH₄ ha⁻¹ y⁻¹ in a dry year to 1.80 kg-CH₄ ha⁻¹ y⁻¹ in a rainy year. Our results suggest that consideration of the soil water conditions across a watershed is important for estimating the CH₄ budgets for entire forest watershed, particularly in regions subject to a wet summer.     

Effect of intermittent drainage in reduction of methane emission from paddy soils in Hokkaido,northern Japan

ABSTRACT

Emission of methane (CH₄), a major greenhouse gas, from submerged paddy soils is generally reduced by introducing intermittent drainage in summer, which is a common water management in Japan. However, such a practice is not widely conducted in Hokkaido, a northern region in Japan, to prevent a possible reduction in rice grain yield caused by cold weather. Therefore, the effects of intermittent drainage on CH₄ emission and rice grain yield have not been investigated comprehensively in Hokkaido. In this study, we conducted a three-year field experiment in Hokkaido and measured CH₄ and nitrous oxide (N₂O) fluxes and rice grain yield to elucidate whether the reduction in CH₄ emission can be achieved in Hokkaido as well as other regions in Japan. Four experimental treatments, namely, two soil types [soils of light clay (LiC) and heavy clay (HC) textures] and two water management [continuous flood irrigation (CF), and intermittent drainage (ID)], were used, and CH₄ and N₂O fluxes were measured throughout the rice cultivation periods from 2016 to 2018. Cumulative CH₄ emissions in 2016 were markedly low, suggesting an initially low population of methanogens in the soils presumably due to no soil submergence or crop cultivation in the preceding years, which indicates a possible reduction in CH₄ emission by introducing paddy-upland crop rotation. Cumulative CH₄ emissions in the ID-LiC and ID-HC plots were 21–91% lower than those in the CF-LiC and CF-HC plots, respectively, whereas the cumulative N₂O emissions did not significantly differ between the different water managements. The amount of CH₄ emission reduction by the intermittent drainage was largest in 2018, with a comparatively long period of the first drainage for 12 days in summer. Rice grain yields did not significantly differ between the different water managements for the entire 3 years, although the percentage of well-formed rice grains was reduced by the intermittent drainage in 2018. These results suggest that CH₄ emission from paddy fields can be reduced with no decrease in rice grain yield by the intermittent drainage in Hokkaido. In particular, the first drainage for a long period in summer is expected to reduce CH₄ emission markedly.     

Agricultural factors affecting methane oxidation in arable soil

CH₄ oxidation activity in a sandy soil (Ardoyen) and agricultural practices affecting this oxidation were studied under laboratory conditions. CH₄ oxidation in the soil proved to be a biological process. The instantaneous rate of CH₄ consumption was in the order of 800 mol CH₄ kg^–1 day^–1 (13 mg CH₄ kg^–1 day^–1) provided the soil was treated with ca. 4.0 mmol CH₄ kg^–1 soil. Upon repeated supplies of a higher dose of CH₄, the oxidation was accelerated to a rate of at least 198 mg CH₄ kg^–1 day^–1. Addition of the plant-growth promoting rhizopseudomonad strains Pseudomonas aeruginosa 7NSK2 and Pseudomonas fluorescens ANP15 significantly decreased the CH₄ oxidation by 20 to 30% during a 5-day incubation. However, with further incubation this suppression was no longer detectable. Growing maize plants prevented the suppression of CH₄ oxidation. The numbers of methanotrophic bacteria and fungi increased significantly after the addition of CH₄, but there were no significant shifts in the population of total bacteria and fluorescent pseudomonads. Drying and rewetting of soil for at least 1 day significantly reduced the activity of the indigenous methanotrophs. Upon rewetting, their activity was regained after a lag phase of about 3 days. The herbicide dichlorophenoxy acetic acid (2,4-D) had a strong negative effect on CH₄ oxidation. The application of 5 ppm increased the time for CH₄ removal; at concentrations above 25 ppm 2,4-D CH₄–oxidizing activity was completely hampered. After 3 days of delay, only the treatments with below 25 ppm 2,4-D showed recovery of CH₄–oxidizing activity. This finding suggests that it can be important to include a CH₄–removal bioassay in ecotoxicology studies of the side effects of pesticides. Changes in the native soil pH also affected the CH₄–oxidizing capacity. Permanent inhibition occurred when the soil pH was altered by 2 pH units, and partial inhibition by 1 pH unit change. A rather narrow pH range (5.9–7.7) appeared to allow CH₄ oxidation. Soils pre-incubated with NH ₄ ⁺ had a lower CH₄–removal capacity. Moreover, the nitrification inhibitor 2-chloro-6-trichloromethyl pyridine (nitrapyrin) strongly inhibited CH₄ oxidation. Probably methanotrophs rather than nitrifying microorganisms are mainly responsible for CH₄ removal in the soil studied. It appears that the causal methanotrophs are remarkably sensitive to soil environmental disturbances.     

Cropland soil–plant systems control production and consumption of methane and nitrous oxide and their emissions to the atmosphere

Croplands are an important source of atmospheric methane (CH₄) and nitrous oxide (N₂O), both potent greenhouse gases. Reduction of cropland CH₄ and N₂O emissions is expected to mitigate climate change. However, large uncertainty remains in the assessment and prediction of these emissions, which prevents us from establishing appropriate mitigation options and strategies. The uncertainty is attributed mainly to the high spatiotemporal variability in emissions (e.g., emission spikes of N₂O). Understanding and quantifying how hotspots of CH₄ and N₂O production in soil and then hot moments of their emissions occur would help reduce the uncertainty. This review focuses on soil–plant systems, particularly the rhizosphere, as possible hotspots of production and consumption of CH₄ and N₂O. It is well known that the rhizosphere controls CH₄ emission strongly, though each process of production and consumption remains to be quantified. On the other hand, surprisingly little attention has been paid to N₂O, besides the fact that plant roots strongly control nitrification and denitrification. We review the current knowledge of cropland CH₄ and N₂O emissions, and conclude that soil–plant interactions strongly affect cropland emissions of both gases, in which functions of plant roots affecting biogeochemical factors (e.g., availability of oxygen, labile organic carbon and inorganic nitrogen) in the rhizosphere and phenological changes are particularly important. In relation to the status of current knowledge, we discuss future research needed.     

太湖地区不同水旱轮作方式下稻季甲烷和氧化亚氮排放研究

为准确编制我国稻田温室气体排放清单及制定合理减排措施提供基础数据,选择太湖地区典型水稻种植区江苏省苏州市,研究设计了休闲水稻(对照,CK)、紫云英水稻(T1)、黑麦草水稻(T2)、小麦水稻(T3)和油菜水稻(T4)5种水旱轮作方式,采用静态箱气相色谱法,开展了不同水旱轮作方式下水稻生长季田间甲烷(CH4)和氧化亚氮(N2O)排放监测试验。试验结果表明:不同水旱轮作方式下水稻生长季CH4排放通量呈先升高后降低的变化趋势,CH4排放峰值出现在水稻生育前期,移栽至有效分蘖临界叶龄期CH4累积排放量占全生育期排放总量的比例为65%~81%,而N2O仅在水稻烤田期间有明显排放。水旱轮作方式对稻季CH4和N2O排放有极显著(P 0.01)影响,CH4季节总排放量表现为T1(283.2 kg.hm 2)CK(139.5 kg.hm 2)T3(123.4kg.hm 2)T4(114.7 kg.hm 2)T2(100.8 kg.hm 2),N2O季节总排放量顺序为T1 T4 T3 T2 CK,依次为1.06kg.hm 2、0.87 kg.hm 2、0.81 kg.hm 2、0.72 kg.hm 2和0.53 kg.hm 2。T1处理稻季排放CH4和N2O产生的增温潜势最高[7 396 kg(CO2).hm 2],显著(P 0.05)高于其他处理,比CK[3 646 kg(CO2).hm 2]增加103%,T2[2 735kg(CO2).hm 2]较CK减少25%(P 0.05)。紫云英水稻轮作方式增加了太湖地区水稻生长季的温室效应。     

Is burial of wheat straw in ditches a way to reduce CH4 emissions from rice cultivation?

Abstract

Burial of wheat straw in ditches and incorporation of wheat straw are the two main ways of returning wheat straw prior to rice cultivation in China. To examine the effect of burying wheat straw in ditches on CH₄ emissions from rice cultivation, a field experiment was conducted at Yixing, Jiangsu, China in 2004. CH₄ flux was measured using a closed-chamber technique in three treatments (CK, no wheat straw application; WI, evenly incorporating 3.75 t ha^?1 wheat straw into the 0.1 m topsoil; WD, burying 3.75 t ha^?1 wheat straw in 0.14-m deep by 0.25-m wide ditches). Seasonal CH₄ emissions ranged from 49.7 to 218.4 kg CH₄ ha^?1. The application of wheat straw in these two ways significantly increased CH₄ emissions by 4.0-fold and 4.4-fold, respectively (P < 0.05). Although CH₄ flux from the non-ditch area in the WD treatment was as low as that in the CK treatment, it was counter-balanced by extremely high CH₄ flux from the ditch, which was approximately 6.0-fold as much as that from WI, leading to comparability between treatments WI and WD in total CH₄ emissions (P > 0.05). No significant difference was observed between the three treatments in grain yield (P > 0.05). The results indicated that burial of wheat straw in ditches is not a way to reduce CH₄ emission from rice cultivation.     

Intermittent irrigation changes production,oxidation, and emission of CH4 in paddy fields determined with stable carbon isotope technique

Intermittent irrigation is an important option for mitigating CH₄ emissions from paddy fields. In order to better understand its controlling processes in CH₄ emission, CH₄ fluxes, CH₄ production and oxidation potentials in paddy soils, and ¹³C-isotopic signatures of CH₄ were observed in field and incubation experiments. The relative contribution of acetate to total CH₄ production (f_ac) and fraction of CH₄ oxidized (f_ox) in the field was also calculated using the isotopic data. At the beginning of the rice season, the theoretical ratio of acetate fermentation: H₂/CO₂ reduction = 2:1 was reached, however, in the late season H₂/CO₂-dependent methanogenesis became dominant. Compared to continuous flooding, intermittent irrigation significantly reduced CH₄ production potential and slightly decreased f_ac-value, indicating methanogens, particularly acetate-utilizing methanogens, were inhibited. CH₄ oxidation was very important, especially in paddy fields under intermittent irrigation where 19–83% of the produced CH₄ was oxidized. Intermittent irrigation enhanced CH₄ oxidation potential slightly and raised f_ox-value significantly relative to continuous flooding. Intermittent irrigation significantly decreased CH₄ flux creating a more positive δ¹³C-value of emitted CH₄ by 12–22‰. A significant negative correlation was found between CH₄ fluxes and values of δ¹³CH₄ suggesting that the less the CH₄ oxidation, the higher the CH₄ emission, and the lower the δ¹³C-value of emitted CH₄. Collectively, the findings show that intermittent irrigation reduced the seasonal CH₄ production potential by 45% but increased the fraction of CH₄ oxidized by 45–63%, thus decreasing the seasonal CH₄ emission from the paddy fields by 71%, relative to continuous flooding.     

Effects of water content and N addition on potential greenhouse gas production from two differently textured soils under laboratory conditions

GHGs production and emission may vary depending on soil physical properties, water management and fertilization. Two paddy soils characterized by different texture were incubated to evaluate the impact of flooding (permanent or intermittent) and N addition on potential N₂O, CH₄ and CO₂ production and release into atmosphere and soil solution. Relationships with volumetric water content (VWC) and water filled pore space (WFPS) were evaluated. Overall, the finer clayey soil (CL) produced 58% more CH₄ than the coarser sandy soil (SA) and showed an earlier sink to source transition; the difference was lower with N addition. Permanent flooding favoured the amount of dissolved CH₄. SA produced more N₂O emissions than CL under permanent flooding (31.0 vs. 3.7%); an opposite pattern was observed for dissolved N₂O (16.4 vs. 52.7%). Fertilization increased N₂O emissions under dry conditions in CL and under flooding in SA.

Our findings showed that i) VWC had a larger influence on N₂O and CH₄ emissions than WFPS, ii) soil type influenced the gas release into atmosphere or soil solution and the timing of sink to source transition in CH₄ emissions. Further investigation on timing of fertilization and drainage are needed to improve climate change mitigation strategies.     

Interactive effects of tree species and soil moisture on methane consumption

Methane consumption by temperate forest soils is a major sink for this important greenhouse gas, but little is known about how tree species influence CH₄ uptake by soils. Here, we show that six common tree species in Siberian boreal and temperate forests significantly affect potential CH₄ consumption in laboratory microcosms. Overall, soils under hardwood species (aspen and birch) consumed CH₄ at higher rates than soils under coniferous species and grassland. While NH₄⁺ addition often reduces CH₄ uptake, we found no effect of NH₄⁺ addition, possibly because of the relatively high ratio of CH₄-to-NH₄⁺ in our incubations. The effects of soil moisture strongly depended on plant species. An increase in soil moisture enhanced CH₄ consumption in soils under spruce but had the opposite effect under Scots pine and larch. Under other species, soil moisture did not affect CH₄ consumption. These results could be explained by specific responses of different groups of CH₄-oxidizing bacteria to elevated moisture.