Effects of season on the reproductive organs and steroid hormone profiles in guinea hens (Numida meleagris)

1. The study documented gross anatomical and histological differences in the reproductive organs of 28 breeding and non-breeding female guinea fowls. Peripheral progesterone and 17β-oestradiol concentrations were also compared in breeding and non-breeding hens.

2. In non-breeding females, all ovarian and oviducal gross anatomical features had significantly regressed. Histologically, some of the changes in a regressing oviduct include systematic changes in height and size of all epithelial cells in all regions of the duct, absence/sparse ciliation of portions of surface epithelium in the magnum, isthmian and uterine regions, general loss of cytoplasmic mass, reduction in size and degeneration of tubular glands. Mucosal folds in all regions of the oviduct except the infundibular lip were higher in breeding females.

3. No difference was found between the two groups in plasma progesterone concentrations. Breeding females, however, had higher peripheral oestradiol concentrations than non-breeding females. About 2 h prior to oviposition, plasma oestradiol concentrations peaked at 2.4-fold (230 pg/ml) compared with baseline concentration and plasma progesterone concentrations by nearly 9-fold (5.29 ng/ml) of baseline.

4. Significant regression and changes in the histological structure of the ovary and oviduct had occurred in non-breeding females, and lower peripheral oestrogen concentrations may be responsible for this phenomenon.

     

An analysis of available techniques for estimating production of pastures without clipping

The management of the predominantly ryegrass‐white clover pastures for milk production in New Zealand is determined by five main factors:

1. the seasonal pattern of dry matter production from pasture, as determined primarily by species composition and the seasonal light and temperature regime;

2. the varying requirements of the pasture plants at different times of the year, as determined by their morphological and physiological condition;

3. the necessity to utilise a large proportion of the dry matter produced if animal production per hectare is to be maximised;

4. late ‘winter calving in over 90% of the dairy herds; and

5. the nutrient requirements of the herd at different stages of lactation.

The aims of maximum production per unit area of land are achieved by an integration of these factors in intensive rotation systems in which the size of the daily break offered to the animals is varied to ration intake and control the frequency and intensity of grazing.     

Free choice feeding of whole grains in meat-type pigeons: 1. effect on performance,carcass traits and organ development

1. The effects of 5 different feeding systems on the performance, carcass traits and organ development were studied in pigeon squabs.

2. The 5 treatments were (1) whole grains of maize, pea and wheat plus concentrate feed; (2)whole grains of maize and wheat plus concentrate feed (CWC); (3) whole grains of maize and pea plus concentrate feed; (4)whole grain of maize plus concentrate feed (CC); and (5) compound feed (CF).

3. Feed intake of parent pigeons increased significantly from 0 to 21 d and it was higher in the CF treatment. Body weight of squabs in the CWC treatment was the highest among the 5 treatments in 4 weeks. Body weight losses of parental pigeons during the rearing period were not significantly different among the 5 treatments.

4. Protein intake in CC and CWC treatments was lower than that of the other three treatments. The CWC treatment had the highest daily weight gain and the lowest feed conversion ratio. Treatments were statistically similar in the relative weight of carcass, breast and thigh. CF had the lower relative weight of abdominal fat.

5. Relative weight of gizzard in the CF treatment was significantly lower than that of CWC.

6. It was concluded that the application of free choice feeding of whole grains of maize and wheat plus concentrate feed increased the body weight of 28-d-old pigeon squabs and decreased the feed conversion rate of parent pigeons. This feeding strategy could be commercially interesting in meat-type pigeon production.

     

Association of MYF5 and KLF15 gene polymorphisms with carcass traits in domestic pigeons (Columba livia)

1. Single nucleotide polymorphisms (SNPs) in the exons of the myogenic factor 5 (MYF5) and Kruppel-like factor 15 (KLF15) genes were identified and analysed by using DNA sequencing methods in 60 female domestic pigeons (Columba livia).

2. Five SNPs (T5067A, C5084T, C5101T, T5127A and C5154G) were detected in exon 3 of MYF5 and 6 SNPs (C1398T, C1464T, G1542A, C1929T, G1965A and A2355G) were found in exon 2 of KLF15, respectively. The analysis revealed three genotypes, in which the AA genotype was dominant and the A allele showed a dominant advantage.

3. For the MYF5 gene, the C5084T and T5127A SNP genotypes were significantly associated with carcass traits of pigeons. Within those two SNPs, the BB genotype showed relatively higher trait association values than those of AA or AB genotypes. No significant association was observed between the KLF15 SNP genotypes and carcass traits.

4. These results indicated that the MYF5 gene is a potential major gene affecting carcass traits in domestic pigeons. The BB genotype of the C5084T and T5127A SNPs could be a potential candidate genetic marker for marker-assisted selection in pigeon.

     

Assessment of the effectiveness of head only and back-of-the-head electrical stunning of chickens

1. The study assesses the effectiveness of reversible head-only and back-of-the-head electrical stunning of chickens using 130–950 mA per bird at 50 Hz AC.

2. Three trials were conducted to compare both stunning systems: (a) behavioural assessment of return of consciousness, (b) insensibility to thermal pain, and (c) assessment of return of brain activity with visually evoked potentials (VEPs).

3. Assessment of behaviour suggested that the period of unconsciousness following head-only electrical stunning was shorter in hens compared to broilers.

4. Stunning across the back-of-the-head delayed the time to return of brainstem function compared to stunning with standard head-only electrodes. Additionally, back-of-the-head stunning produced a more prolonged period of electroanalgesia compared to head-only.

5. Based on examination of return of brain function with VEPs in hens, back-of-the-head stunning produced a shorter-lasting stun than standard head-only. However, even for standard head-only, the stun was notably shorter than previously reported. In some birds, brain function had returned within 9 s after the end of stunning.

6. The results suggest that some birds may recover consciousness prior to or during the neck cut. Based on these findings, back-of-the-head stunning and standard head-only stunning of hens should not be recommended without further development.

     

Histopathological studies of the heart in three lines of broiler chickens

1. The aim of this study was to analyse the morphological lesion pattern of the heart of broiler chickens (Cobb 500, Hubbard F15 and Ross 308) during fattening with no clinical signs of disease and to determine the most susceptible period for the occurrence of morphological lesions.

2. The most frequently diagnosed lesions in each genetic line were degeneration of the fibres with vacuolation, congestion of cardiac muscle, oedema and vacuolisation of the Purkinje cells.

3. The highest numbers of morphological lesions were observed on d 38, 31 and 10 of life. The lesions were most numerous in the septum, followed by the left and right ventricles.

4. Ischaemic cardiomyocytes were also most numerous on d 38 of life and in the left ventricle.

5. Overload of cardiac muscle, prolonged hypoxia and increasing body weight on d 38 are the likely reasons for the largest number of lesions and ischaemic fibres, which may lead to heart failure.

     

The spring-rise phenomenon: Seasonal changes in the worm burdens of breeding ewes and in the availability of pasture infection

Extract

A Mastitis control programme is essentially a biological regulatory system involving the three essential components of any control mechanism:

• A signal from a detector indicating departure from normality

• The interpretation of the signal (diagnosis)

• A feed-back signal to the origin of the detection signal indicating corrective action.

     

The Hen's egg: Factors affecting the shearing strength of shell material

Experiments are described from which it is concluded that

1. the force required to shear egg shell material can be measured with high precision by pressing a narrow, flat‐ended plunger on to the shell;

2. shearing force falls and the precision of its measurement rises as the radius of the plunger is reduced;

3. shearing force is related linearly to shell thickness ;

4. the regression line of shearing force on shell thickness does not pass through the origin but has a substantial positive intercept on the thickness axis, at about a third of the mean thickness ;

5. there are differences between hens and between strains of hen in the magnitude of the intercept, but not in the slope of the regression;

6. shearing force is affected also by the glossiness of the shell;

7. shearing force was not significantly affected by stripped membrane thickness, number of mammillae per unit area of shell, shell organic content (standardised for shell thickness), shell curvature, shell colour, mottling score, translucent patch score, speckling score, age of the egg or age of the hen that laid it;

8. measured with a plunger of radius 165 μm, the mean shearing strength of incremental shell was 57 kg wt/mm² (3–7 ton/in²).

The findings suggest that the shearing strength of egg shells might be increased by direct selection and by selection for shell glossiness.     

Gender-related amino acid intake of adult wild Red-legged Partridge (Alectoris rufa)

1. The Red-legged Partridge (Alectoris rufa) is under an enormous hunting pressure. It is bred intensively in game farms. The hunting season is during the non-reproductive resting period when partridges are at maintenance conditions.

2. There is a lack of information about the amino acid (AA) composition of the natural diet of the adult birds in their habitat or differences in dietary AA composition related to gender. The objective of this work was to establish a first approach to the AA composition of the natural diet of adult wild Red-legged Partridge.

3. Food content in crops and gizzards of female and male birds hunted in the same hunting season and area was analysed for AA composition.

4. Females food had higher concentrations of individual essential AA (EAA) and non-essential AA (NEAA) than males.

5. There are important differences in the concentration of AA in the natural diet of wild females and males. It may be advisable to use diets differing in the proportion of individual AA in the game farms during the non-reproductive resting period.

     

Serological and haematological values in geriatric broad-breasted white turkeys

1. Blood samples from 1-, 5- and 10-year-old broad-breasted white turkeys were used to determine haematological and serum values. There were 8 turkeys in each age group.

2. Mean haematocrit, haemoglobin and creatinine values were higher in the 1-year-old than in 10-year-old turkeys.

3. Mean alanine aminotransferase was greater in 1 year old than in 10 year olds and greater in 5 year olds than in 10 year olds. Aspartate aminotransferase values were significantly different between all age groups.

4. Mean alkaline phosphatase was less in 1-year-old than in 10-year-old turkeys. There were no significant differences between the three groups for cholesterol, glucose, total protein, albumin, globulin, calcium and bilirubin.

5. All male turkeys had higher haemoglobin and haematocrit values than age-matched females.

6. These data will help establish basic background references values in geriatric turkeys used in research.

     

Estimation of genetic parameters related to eggshell strength using random regression models

1. This study examined the changes in eggshell strength and the genetic parameters related to this trait throughout a hen’s laying life using random regression.

2. The data were collected from a crossbred population between 2011 and 2014, where the eggshell strength was determined repeatedly for 2260 hens.

3. Using random regression models (RRMs), several Legendre polynomials were employed to estimate the fixed, direct genetic and permanent environment effects. The residual effects were treated as independently distributed with heterogeneous variance for each test week.

4. The direct genetic variance was included with second-order Legendre polynomials and the permanent environment with third-order Legendre polynomials.

5. The heritability of eggshell strength ranged from 0.26 to 0.43, the repeatability ranged between 0.47 and 0.69, and the estimated genetic correlations between test weeks was high at > 0.67.

6. The first eigenvalue of the genetic covariance matrix accounted for about 97% of the sum of all the eigenvalues.

7. The flexibility and statistical power of RRM suggest that this model could be an effective method to improve eggshell quality and to reduce losses due to cracked eggs in a breeding plan.

     

Characterisation of caecum and crop microbiota of Indian indigenous chicken targeting multiple hypervariable regions within 16S rRNA gene

1. A comparative analysis of caecum and crop microbiota of chick, grower and adult stages of Indian indigenous chickens was conducted to investigate the role of the microbiota of the gastrointestinal tract, which play an important role in host performance, health and immunity.

2. High-throughput Illumina sequencing was performed for V3, V4 and V4-V6 hypervariable regions of the 16S rRNA gene. M5RNA and M5NR databases under MG-RAST were used for metagenomic datasets annotation.

3. In the crop, Firmicutes (~78%) and Proteobacteria (~16%) were the predominant phyla whereas in the caecum, Firmicutes (~50%), Bacteroidetes (~29%) and Actinobacteria (~10%) were predominant. The Shannon-Wiener diversity index suggested that sample richness and diversity increased as the chicken aged.

4. For the first time, the presence of Lactobacillus species such as L. frumenti, L. antri, L. mucosae in the chicken crop along with Kineococcus radiotolerans, Desulfohalobium retbaense and L. jensenii in the caecum are reported. Many of these bacterial species have been found to be involved in immune response modulation and disease prevention in pigs and humans. The gut microbiome of the indigenous chicken was enriched with microbes having probiotic potential which might be essential for their adaptability.

     

Influences of end-point heating temperature on the quality attributes of chicken meat

1. This study aimed to evaluate the changes in physicochemical, textural and sensory characteristics of broiler (BR) and Taiwan native chicken (TNC) pectoralis muscle heated at temperatures of 50–95°C.

2. With increasing temperature, cooking loss, collagen solubility, shear force value and hardness, of samples increased in both chicken breeds. Rapid decreases in protein solubility were observed when the meat was heated to 50°C and gradually decreased thereafter.

3. Meat from BRs and native chickens performed differently upon heating in certain characteristics. TNC meat had longer cooking time and lower myofibrillar fragmentation index than BR meat did. TNC meat had higher collagen content, shear force values and springiness but lower collagen solubility than BR meat did.

4. BR meat had significantly higher onset and end transition temperatures than TNC meat did. In BR meat, a sensory analysis revealed that moisture release decreased and chicken flavour increased with increasing temperature.

5. Protein solubility, cooking loss and the texture of heated meat were highly correlated.

6. This study scientifically assessed the performances of the two breeds of chickens with different thermal treatments; producers could utilise the information to produce poultry products with more desirable qualities.

     

Changes in eggshell structure and predisposition of broilers to health problems: is there a common pathophysiology?

1. A study of broiler breeder eggs differing in eggshell matrix optical density was conducted to determine the association of eggshell structural quality and the risk of disease in broilers.

2. A total of 10 000 eggs from a broiler breeder flock were examined according to the pre-established criteria, and allocated to groups classified as having a high or low density shell matrix.

3. The eggs from respective groups were incubated and hatched in a commercial hatchery. Samples of unhatched eggs from each group were subjected to detailed examination to establish the cause of reproductive failure. First, quality chicks from each group were raised as separate flocks in a commercial broiler barn. Group performance, morbidity, and mortality were monitored throughout the growth period. All birds were processed in a commercial plant, and condemnation data were compiled.

4. There were significantly more unhatched eggs, and fewer quality chicks in the group classified as having a low density eggshell matrix, in comparison to the high-density group.

5. Embryo pathology accounted for a large proportion of the overall reproductive failure in both groups, with a large proportion of embryos showing anatomical anomalies. The eggs and embryos from the low-density eggshell matrix group were three times more likely to be infected.

6. Significantly higher production losses associated with mortality/morbidity, and condemnations of carcasses at processing were observed in broilers from the low-density eggshell group compared with the high-density eggshell group.

7. It is concluded that common metabolic/physiological changes in breeder hens associated with eggshell pathology may be also risk factors linked with predisposition of broiler chicks to some health problems.

     

Investigation of laboratory testing issues in the context of the Salmonella National Control Programme in Great Britain

1. The objective of this study was to identify any issues arising during the laboratory testing of samples collected under the National Control Programme for Salmonella.

2. A questionnaire-based survey was conducted among Defra (Department for Environment, Food and Rural Affairs)-approved testing laboratories in order to identify any complicating factors that the laboratories may encounter during the processing of samples.

3. Samples were reported to arrive in good condition and within the specified time frame after collection. The only concern was that new clients may be unaware of the procedure or correct sampling consumables to use.

4. There was evidence of variability between laboratories regarding the sample testing procedure used, with deviation from the guidelines in some cases.

5. This finding suggests that further guidance for laboratories on methodology is likely to be beneficial as this could help improve the detection of low levels of Salmonella.

     

Effects of medium-chain fatty acids on performance,carcass characteristics,blood biochemical parameters and immune response in Japanese quail

1. This study had the aim of evaluating the effects of medium-chain fatty acids (MCFA) on performance, carcass characteristics, some blood parameters and antibody titre against sheep red blood cells (SRBC) in quail.

2. A total of 240 quail chicks were allotted to 4 treatments consisting of respectively 0, 1, 2 and 4 g/kg dietary MCFA.

3. There were no significant differences in body weight, feed intake and feed conversion ratio among treatments at different stages of the experiment.

4. MCFAs had no significant effect on breast, thigh, liver, spleen and bursa of Fabricius weight ratios. However, the relative weight of abdominal fat significantly decreased in quail receiving 0.2 and 0.4 MCFA as compared to other treatments.

5. Concentrations of low-density lipoprotein-cholesterol (LDL), triglycerides and total cholesterol were decreased and high-density lipoprotein (HDL)-cholesterol was increased in quail chicks receiving MCFA compared with control quail chicks. The concentrations of SRBC antibody were not statistically different among treatments.

6. It is concluded that MCFA significantly decreased LDL, triglycerides, cholesterol and abdominal fat and increased HDL in quail chicks.

     

Abusive use of antibiotics in poultry farming in Cameroon and the public health implications

1. The types and methods of use of antibiotics in poultry farms in Cameroon, residual levels and potential microbial resistance were determined.

2. A questionnaire-based survey identified the different antibiotics used and high-performance liquid chromatography (HPLC) was used to determine residual levels of antibiotics.

3. Pathogens were isolated, identified by use of commercial API kits and minimum inhibition concentration (MIC) was determined.

4. Oxytetracyclin, tylocip and TCN (oxytetracycline, chloramphenicol and neomycin) were the most frequently used antibiotics. Antibiotics screened by HPLC were chloramphenicol, tetracycline and vancomycin. All of them except vancomycin were detected, and the concentration of these antibiotics was higher than the maximum residual limits (MRL) set by regulatory authorities.

5. No residues of various antibiotics were found in egg albumen or yolk. The concentration of tetracycline was significantly higher in liver (150 ± 30 µg/g) than in other tissues.

6. Foodborne pathogens, including Salmonella spp., Staphylococcus spp., Listeria spp., Clostridium spp. and Escherichia spp., were identified. Most of the pathogens were resistant to these various antibiotics tested.

7. These findings imply the need for better management of antibiotic use to control sources of food contamination and reduce health risks associated with the presence of residues and the development of resistant pathogens by further legislation and enforcement of regulations on food hygiene and use of antibiotics.

     

Screening of Lactobacillus isolates from gastrointestinal tract of guinea fowl for probiotic qualities using in vitro tests to select species-specific probiotic candidates

1. A total of 32 Lactobacillus isolates, 8 each from the crop (LGFCP1–LGFCP8), proventriculus (LGFP9–LGFP16), ileum (LGFI17–LGFI24) and caeca (LGFCM25–LGFCM32) were isolated from 25 adult guinea fowl (Pearl variety), 22–28 weeks of age, and characterised morphologically, physiologically, biochemically and by molecular methods.

2. Isolates were screened for their probiotic quality using range of in vitro tests: aggregation test, cell surface hydrophobicity, resistance to bile salts and acidic conditions, enzymatic tests and coaggregation and antagonistic test.

3. Based on in vitro test results and a novel scoring method, the two best isolates were selected and partial 16S rRNA sequencing was done. BLAST (Basic Local Alignment Search Tool) analysis of sequence of isolate LGFCP4 showed 99% genetic identity with Lactobacillus plantarum and LGFP16 with Lactobacillus reuteri.

4. The study shows that these two microbial agents may be suitable as potential probiotic candidates in guinea fowl, as well as in a feed supplement for other poultry species.

     

The correlation between height of thick albumen,yolk diameter,shape index and the position of the yolk after storage

From about 100 White Leghorn hens the following characteristics were determined on samples of four eggs per hen:

1. The shift of the yolk during a storage period of 7 d at a temperature of 20 °G

2. The height of the thick albumen directly after lay, and at the end of the storage period

3. The diameter of the yolk

4. The shape index of the egg.

The correlation, on a hen basis, between the shift of the yolk and the other above‐mentioned traits was only of very minor importance. This means that one of the arguments on the basis of which the height of the thick albumen was used as a criterion for the internal quality of eggs—the influence of the thick white on the central position of the yolk—has lost much of its significance.

In addition the same measurements were made on eggs from 12 entries at the Random Sample Testing Station at Putten. Here samples of 20 eggs per entry were taken. Contrary to expectation the combination of a large amount of thick albumen and a large shift of the yolk proved to be the rule rather than the exception.     

Critical test evaluation of micronized mebendazole against Anoplocephala Perfoljata in the horse

Extract

More than half of the writer's group practice involves equine cases, mostly racehorses, and a large percentage of the surgery associated with them is orthopaedic. For this reason, and because a prosthetic technique requiring good surgical facilities has been used for surgical correction of laryngeal hemiplegia, an attempt was made to design and have constructed a unit which would fulfil the following :

2. A table of suitable size and strength with adequate padding.

3. The table to be elevated reliably to a comfortable operating height.

4. The elevation to be; rapid so that after induction of anaesthesia and elevation the horse would still be deeply anaesthetized enough to allow easy passage of the endotracheal tube.

5. A safe and efficient method to move the anaesthetized post-operative patient to a recovery room.

The capital and maintenance costs of the theatre had to be met by the returns from surgical fees and, to avoid increasing these significantly, the construction was carried out using facilities already available, and using only essential items.