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1.
Summary Wheat doubled haploid (DH) lines were produced from the F1 hybrid, Fukudo-komugi x Oligo Culm, through intergeneric crosses between wheat and maize. F2 plants and 203 DH lines were analyzed for the segregation of the eight genetic markers, namely, grain proteins, grain esterases, GA-insensitivity and glume traits. The segregation in the F2 plants fitted to the expected ratios. No deviation was observed among the DH lines, either, except for the glume pubescence. The result indicates the absence of correlation between the markers investigated and the efficiency of embryo formation in the DH lines. 相似文献
2.
Haitham Sayed Gunter Backes Hamed Kayyal Amor Yahyaoui Salvatore Ceccarelli Stefania Grando Ahmad Jahoor Michael Baum 《Euphytica》2004,135(2):225-228
A partial genetic linkage map was constructed on 71 doubled-haploid lines derived from a cross between the barley lines Tadmor
and WI2291 with 181 molecular markers. The segregating population was used to detect markers linked to the gene Mlg conferring resistance to powdery mildew (Erysiphe graminis f. sp. hordei) and to genes for quantitative resistance to scald (Rhynchosporium secalis). The gene Mlg on chromosome 4H was flanked by two AFLP markers at a distance of 2.0 and 2.4 cM, respectively. QTLs for resistance to scald
were detected on chromosomes 2H and 3H. This association of molecular markers with qualitative and quantitative disease resistance
loci represents a valuable starting-point for marker-assisted selection.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Summary The segregation of 12 heterozygous isozyme markers was analyzed among F2 plants and 51 anther culture (AC)-derived lines obtained from the japonica × indica cross of rice, IRAT 177 × Apura. All the lines except two were homozygous products of recombination of the two parental phenotypes. Doubled haploid (DH) lines derived from plants regenerated from the same callus were identical, confirming previously obtained results in rice. Surprisingly, some lines derived from different calli were also identical, suggesting a phenomenon of early callus fragmentation. All these observations at the isozyme level were confirmed by field evaluation. Deviations of segregations from the expected 1 : 1 ratio were observed at 4 loci among the DH lines. Among these, two were also noted among the F2 plants. The two other distortions, both in favor of the japonica allele, were observed specifically in the AC-derived materials.Although this concerns a small proportion of the genes under study, it suggests that the embryogenic microsporal population does not represent a random gametic array. On the other hand, evaluation of recombination between isozyme genes located on chromosome 6 appears consistent with F2 data and data previously recorded on the other japonica × indica crosses. The potential use of isozymes in breeding doubled haploids derived from remote crosses in rice is discussed.Abbreviations MCPA =
2-methyl-4-chlorophenoxyacetic acid
- IAA =
indolacetic acid
- AC plant or line =
anther culture-derived plant or line
- DH line =
doubled haploid line 相似文献
4.
Quantitative trait loci for grain yield and yield components in a cross between a six-rowed and a two-rowed barley 总被引:8,自引:0,他引:8
Summary The positions of quantitative trait loci (QTL) for yield and yield components were estimated using a 85-point linkage map and phenotype data from a F1-derived doubled haploid (DH) population of barley. Yield and its components were recorded in two growing seasons. Highly significant QTL effects were found for all traits at several sites in the genome. A major portion of the QTL was found on chromosome 2. The effect of the alleles in locus v on thousand grain weight and kernels per ear explained 70–80% of the genetic variation in the traits. QTL × year interaction was found for grain yield. Several different QTL were found within the two-rowed DH lines compared to those found in the six-rowed DH lines. Epistasis between locus v and several loci for yield and yield components indicates that genes are expressed differently in the two ear types. This may explain the difficulties of selecting high yielding lines from crosses between two-rowed and six-rowed barley.Abbreviations DH
doubled haploid
- QTL
quantitative trait locus/loci
- RAPD
random amplified polymorphic DNA
- RFLP
restriction fragment length polymorphism
- T. Prentice
Tystofte Prentice
- V. Gold
Vogelsanger Gold 相似文献
5.
Quantitative trait loci for scald resistance in barley localized by a non-interval mapping procedure 总被引:1,自引:0,他引:1
Three quantitative trait loci (QTL) for scald resistance in barley were identified and mapped in relation to molecular markers using a population of chromosome doubled‐haploid lines produced from the F1 generation of a cross between the spring barley varieties ‘Alexis’ and ‘Regatta’. Two field experiments were conducted in Denmark and two in Norway to assess disease resistance. The percentage leaf area covered with scald (Rhynchosporium secalis) ranged from 0 to 40% in the 189 doubled‐haploid (DH) lines analysed. One quantitative trait locus was localized in the centromeric region of chromosome 3H, Qryn3, using the MAPQTL program. MAPQTL was unable to provide proper localization of the other two resistance genes and so a non‐interval QTL mapping method was used. One was found to be located distally to markers on chromosome 4H (Qryn4) and the other, Qryn6, was located distally to markers on chromosome 6H. The effects of differences between the Qryn3, Qryn4 and Qryn6 alleles in two barley genotypes for the QTL were estimated to be 8.8%, 7.3% and 7.0%, respectively, of leaf covered by scald. No interactions between the QTLs were found. 相似文献
6.
The inheritance of resistance to fusarium wilt (Fusarium oxysporum f.sp. lini) was investigated in Linum usitatissimum as
a first step towards gaining an understanding of the molecular genetics of the disease and developing a procedure for marker-assisted
selection. A recombinant doubled haploid (DH) population was derived from the haploid component of polyembryonic F2 seeds originating from a cross between a wilt resistant, twinning Linola™ Linola is a registered trademark of CSIRO line
CRZY8/RA91 and the wilt susceptible Australian flax cultivar Glenelg. The segregation of resistance was studied in 143 DH
lines under glasshouse and field conditions. Most of the phenotypic variation was attributable to the segregation of two independent
genes with additive effects. Minor resistance genes may have also contributed by modifying the resistance response. A glasshouse
screening method of DH lines proved a reliable indicator of field resistance to fusarium wilt.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
7.
Quantitative trait loci (QTLs) for resistance against non-parasitic leaf spots (NPLS) were first characterized in a spring barley double haploid population derived from the cross IPZ 24727/Barke (Behn et al., 2004). The aim of the present study was to identify QTLs for NPLS resistance in the half-sibling DH population IPZ 24727/Krona and to compare them with the QTLs of the population IPZ 24727/Barke. An anther culture-derived doubled haploid population of 536 DH lines was developed from the cross IPZ 24727 (resistant)/Krona (susceptible). Field trials were performed over two years in two replications, scoring NPLS and agronomic traits that might interact with NPLS. A molecular linkage map of 1035 cM was constructed based on AFLPs, SSRs and the mlo marker. QTL analyses for NPLS identified three QTLs that accounted for 30% of the phenotypic variation. For comparison of the QTLs from each DH population, a consensus map was generated comprising 277 markers with a length of 1199 cM. In both populations, the QTLs for NPLS mapped to chromosomes 1H, 4H and 7H. A common QTL with a great effect in both populations and over all environments was localized at the mlo locus on chromosome 4H, indicating that the mlo powdery mildew resistance locus has a considerable effect on NPLS susceptibility. The steps necessary to validate the QTLs and to improve the NPLS resistance by breeding were discussed. 相似文献
8.
Greenbug is one of the most aggressive pests of barley and wheat. In Argentina, yield losses of wheat, barley, oat and sorghum crops caused by greenbug are chronic and at times severe. Since Marker Assisted selection for greenbug resistance genes in barley is very limited, the purpose of the current study was to map greenbug resistance genes in doubled haploid (DH) lines and to identify candidate genes. A set of DH lines of the Oregon-Wolfe Barley (OWB) mapping population derived from the cross between OWBDOM and OWBREC and both parental lines were screened for tolerance to greenbug. There was significant variation among the DH lines in foliar area (FA), dry weight (DW) and chlorophyll contents (Ch) between infested and control DH lines. Three main QTLs were identified. These QTLs explained 82 % of the FA, 80 % of DW and 58 % of Ch variability of infested plants. The initial and final FA and DW of controls and final DW of infested plants were associated with the same molecular markers on chromosome 2H (Vrs1, BmAc0144f, GBR259, GBS705). The final FA of infested plants was significantly linked to molecular markers on chromosome 5H (GBRO986, GBR518, GBM1483, GBR1082). The positive alleles were provided by OWBDOM. The content of chlorophyll of infested plants was associated with the marker loci Ris44, GBR1608, GBR1637N and GBS0785 on chromosome 7H, with the positive alleles provided by OWBREC. Both parents contributed to different tolerance traits. The QTLs found in this population are new greenbug resistance loci. A sequence homology search was performed to derive the putative function of the genes linked to the QTLs. 相似文献
9.
The aim of the study was to evaluate the relationship between genetic and phenotypic distances of parents and the genetic potential of crosses as measured by the frequency of transgressive segregants in homozygous populations. Material for the study involved 17 barley cross‐combinations. In each cross, the parental genotypes, F2 hybrids and doubled haploid (DH) lines were analysed. Yield and yield‐related traits were observed in the experiments. Phenotypic (univariate and multivariate) and genetic distances (GD) were investigated between pairs of parental genotypes. Genetic distance was evaluated by using random amplified polymorphic DNA markers. In F2 generations, the genetic coefficient of variability (GCV) was evaluated. Within all the cross‐combinations studied, each DH line was compared with both parents to distinguish the positive and negative transgressive lines. In addition, the coefficient of gene distribution (r) along parental genomes was evaluated. Relationships between frequency of transgression and both phenotypic and GDs, GCV and r, were assessed by regression analysis. It was found that for all the traits studied the frequency of transgressive lines depended mainly on gene distribution (r). Genetic distance between parents appeared to be significant for the occurrence of transgression effects in plant height, ear length, grain weight per ear and grain yield per plot. Regression analysis has shown that phenotypic differences between parental genotypes were also important for the frequency of transgressive lines. A weak relationship was found between the variation of F2 hybrids and the occurrence of transgressive lines. The results indicate that occurrence of transgressive segregants in a homozygous population should be considered as a phenomenon dependent simultaneously on several factors characterizing parental genotypes. Among them, the most important are: gene distribution, phenotypic diversity and GD. 相似文献
10.
Justyna Guzy-Wróbelska Anna Labocha-Pawlowska Miroslaw Kwasniewski Iwona Szarejko 《Euphytica》2007,156(1-2):173-183
This study compared the meiotic recombination frequency between wheat doubled haploid (DH) populations obtained through two
different methods, maize pollination (MP♀) and anther culture (AC♂). The comparison was based on a genetic linkage analysis,
performed with DNA markers. Thirty-five polymorphic markers (15 SSR, 15 AFLP, 5 RAPD) were screened in MP♀ and AC♂ doubled
haploids populations, derived from the same hybrid genotype (F1 of ‘Eta’ × ‘Darkhan 15’). Nine linkage groups, comprising 35 loci (the MP♀ lines) and 31 loci (the AC♂ lines), were constructed.
The linkage groups in both DH populations showed identical orders of markers, except for one group mapping to chromosome 6B.
The MP♀ and AC♂ linkage maps differed significantly in recombination frequencies for corresponding intervals. In total, the
AC♂ linkage map (495.5 cM) was 40.5% longer than the MP♀ map (352.8 cM), indicating a significantly higher meiotic recombination
rate in pollen mother cells. The enhancement in recombination was visible in five of nine linkage groups, and in 7 intervals
between individual loci out of 19 compared. Moreover, for 6 other intervals a lack of linkage was observed in the AC♂ population,
as compared to the MP♀ map. 相似文献
11.
Hidetoshi Ajisaka Yasuhisa Kuginuki Susumu Yui Sueo Enomoto Masashi Hirai 《Euphytica》2001,118(1):75-81
DNA markers linked to a locus controlling an extreme late bolting trait, which was originally found in a local cultivar of
a non-heading leafy vegetable,‘Osaka Shirona Bansei’ (Brassica rapa L. ssp. pekinensis syn. campestris L.) were identified using bulked segregant analysis. A doubled haploid (DH) line, DH27, which is a progeny of ‘Osaka Shirona
Bansei’, shows extreme late bolting, and bolts without vernalization. DH27 was crossed with a normal bolting DH line, G309.
The plantlets of the parents, F1 and F2, were vernalized and then grown in a greenhouse. The bolting time of F2 plants showed a continuous distribution from 19 to 231 days after vernalization (DAV), suggesting the effects of a few major
genes and polygenes. Possible linkage markers for this trait were screened by modified bulked segregant analysis (BSA). The
BSA using four bulks suggested that a 530-bp RAPD band RA1255C was linked to a locus controlling the bolting trait. The RAPD
band was cloned and used as a probe to detect RFLP. The fragment detected a single locus, BN007-1,the segregation of which
in the F2 population matched that of RA1255C. Three other RAPDs were found to be linked to BN007-1. A quantitative trait locus(QTL)
affecting the bolting time was detected around BN007-1 using MAPMAKER/QTL. Since the difference between bolting times of both
the parental genotypes in the F2 was 138 days, these markers may be useful for a marker-assisted selection (MAS) in the breeding program for late bolting
or bolting-resistant cultivars in B. rapa crops.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
12.
RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. 总被引:10,自引:0,他引:10
Yasuhisa Kuginuki Hidetoshi Ajisaka Mamiko Yui Hiroaki Yoshikawa Ken-ichi Hida Masashi Hirai 《Euphytica》1997,98(3):149-154
Linkage of random amplified polymorphic DNA (RAPD) markers with resistance genes to clubroot (Plasmodiophora brassicae Wor.)
in Brassica rapa L. was studied in a doubled haploid (DH population obtained by microspore culture. Thirty-six DH lines were
obtained from F1 plants from a cross between susceptible ‘Homei P09’ and resistant ‘Siloga S2’ plants. ‘Homei P09’ was a DH line obtained
by microspore culture of the Chinese cabbage variety ‘Homei’, which is highly responsive in microspore culture. The resistant
line ‘Siloga S2’ was obtained by two rounds of selfing of the fodder turnip ‘Siloga’. Three RAPD markers, RA12-75A, WE22B
and WE49B, were found to be linked to a clubroot-resistance locus. These three markers were linked in the DH lines and an
F2 population and should be useful for marker-assisted selection in breeding programs.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
13.
A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.) 总被引:1,自引:0,他引:1
Qiaojun Lou Liang Chen Zongxiu Sun Yongzhong Xing Jun Li Xiaoyan Xu Hanwei Mei Lijun Luo 《Euphytica》2007,158(1-2):87-94
Chilling injury is one of the most important limiting factors affecting rice production in temperate and high-elevation areas.
In this study, 146 microsatellite markers were employed to identify quantitative trait loci (QTL) conferring cold tolerance
at seedling stage (CTS) .The mapping population consisted of 193 doubled haploid (DH) lines, which derived from a cross between
a cold-tolerant japonica variety (AAV002863) and a cold-sensitive indica cultivar (Zhenshan97B). Tolerance to cold was assessed by the survival percentage of seedlings after cold treatment. In a
climate chamber, after treatment at 6°C/10°C for 7 d, the measurement was taken on the sixth day of the recovery stage at
room temperature. The phenotypic distribution of the DH population approximately fitted normality with skewness and kurtosis
less than 0.3, and the difference among the three repetitions was not significant. Five main effect QTLs were identified with
LOD > 4.0 on chromosomes 1, 2, 8 using a composite interval mapping approach. The accumulated contribution of the five QTLs
was 62.28%, and a major QTL (LOD = 15.09) was identified on chromosome 2 flanked by RM561 and RM341, which explained 27.42%
of the total phenotypic variation. Four significant epistatic interactions were also detected with a total contribution of
20.14%.
Liang Chen and Qiaojun Lou had made the equal contribution for the research. 相似文献
14.
Fat content is a concern for the enhancement of rice for eating, cooking, and storage qualities. To clarify its genetic mechanism,
a double haploid (DH) population derived from anther hybrid F1 of Zhenshan 97B (indica) and Wuyujing 2 (japonica) and two backcross F1 (BCF1) populations, which came from the DH lines backcrossing to two parents, were used to scan quantitative trait loci (QTLs)
and dissect gene effects for the crude fat content (CFC) in brown rice. Fourteen QTLs were resolved, distributing on chromosomes
1, 3, and 5–9. Three loci were detected repeatedly in two populations, DH or BCF1. Among these loci, a major QTL, qCFC5, flanking markers RM87 and RM334, was located on chromosome 5, which was detected simultaneously among three populations.
The main QTLs had a major role in controlling CFC in brown rice and were modified by several mini-effect QTLs and epistatic
affection.
Wenjun Liu and Jing Zeng are contributed equally to this paper. 相似文献
15.
E. Tocho M. Ricci M. S. Tacaliti D. O. Giménez A. Acevedo U. Lohwasser A. B?rner A. M. Castro 《Euphytica》2012,188(2):239-251
The Russian wheat aphid (RWA) is one of the most aggressive pests of barley and wheat. The outbreak of RWA occurred in Argentina in 2008 caused serious damage to barley cultivars. The most effective and sustainable method of RWA control is to identify new resistance genes. The purpose of the current research was to map RWA resistance genes in a set of double haploid (DH) lines of the Oregon-Wolfe Barley (OWB) mapping population derived from the cross between OWBDOM and OWBREC. The DH and both parental lines were screened for antixenosis, tolerance and antibiosis to RWA. There was significant variation among the DH lines in most of the traits studied. However, only tolerance resulted in significant quantitative trait loci (QTLs) associated with the molecular markers. Two main QTLs were identified. These explained 90 and 79 % of the variability of foliar area and chlorophyll content, respectively, of infested and control plants. The initial and final foliar area and the variation in foliar area were associated with the same molecular markers on chromosome 2H (BmAc0125, Vrs1, BmAc0144f and BmAg0113e). The positive alleles were provided by OWBDOM. The content of chlorophyll was associated with the marker loci WMC1E8, MWG912, ABC261, MWG2028 and Blp on chromosome 1H, with the positive alleles provided by OWBREC. Both parents contributed to different tolerance traits, with foliar area and chlorophyll content remaining as the plant traits most affected by aphid feeding. The QTLs found in this population are new RWA resistance loci. A sequence homology search was performed to derive the putative function of the genes linked to the QTLs. 相似文献
16.
Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus) 总被引:1,自引:0,他引:1
Zhen Li Shufang Mei Zhong Mei Xianglei Liu Tingdong Fu Guangsheng Zhou Jinxing Tu 《Euphytica》2014,197(3):341-353
Soil waterlogging and drought are major environmental stresses that suppress rapeseed (Brassica napus) growth and yield. To identify quantitative trait loci (QTL) associated with waterlogging tolerance and drought resistance at the rapeseed seedling stage, we generated a doubled haploid (DH) population consisting of 150 DH lines from a cross between two B. napus lines, namely, line No2127-17 × 275B F4 (waterlogging-tolerant and drought-resistant) and line Huyou15 × 5900 F4 (waterlogging-sensitive and drought-sensitive). A genetic linkage map was constructed using 183 simple sequence repeat and 157 amplified fragment length polymorphism markers for the DH population. Phenotypic data were collected under waterlogging, drought and control conditions, respectively, in two experiments. Five traits (plant height, root length, shoot dry weight, root dry weight and total dry weight) were investigated. QTL associated with the five traits, waterlogging tolerance coefficient (WTC) and drought resistance coefficient (DRC) of all the traits were identified via composite interval mapping, respectively. A total of 28 QTL were resolved for the five traits under control conditions, 26 QTL for the traits under waterlogging stresses and 31 QTL for the traits under drought conditions. Eleven QTL were detected by the WTC, and 19 QTL related to DRC were identified. The results suggest that the genetic bases of both waterlogging tolerance and drought resistance are complex. Some of the QTL for waterlogging tolerance-related traits overlapped with QTL for drought resistance-related traits, indicating that the genetic bases of waterlogging tolerance and drought resistance in the DH population were related in some degree. 相似文献
17.
Jorge Luis Fuentes Fernando José Correa-Victoria Fabio Escobar Gustavo Prado Girlena Aricapa Myriam Cristina Duque Joe Tohme 《Euphytica》2008,160(3):295-304
The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from
the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant and susceptible families from F3 segregating populations of a cross between the near-isogenic lines C101LAC (resistant) and C101A51 (susceptible) were used
to identify polymorphic microsatellite markers associated to this gene through bulked segregant analysis. Putative molecular
markers linked to the blast resistance gene Pi-1(t) were then used on the whole progeny for linkage analysis. Additionally, the diagnostic potential of the microsatellite markers
associated to the resistance gene was also evaluated on 17 rice varieties planted in Latin America by amplification of the
specific resistant alleles for the gene in each genotype. Comparing with greenhouse phenotypic evaluations for blast resistance,
the usefulness of the highly linked microsatellite markers to identify resistant rice genotypes was evaluated. As expected,
the phenotypic segregation in the F3 generation agreed to the expected segregation ratio for a single gene model. Of the 24 microsatellite sequences tested, six
resulted polymorphic and linked to the gene. Two markers (RM1233*I and RM224) mapped in the same position (0.0 cM) with the
Pi-1(t) gene. Other three markers corresponding to the same genetic locus were located at 18.5 cM above the resistance gene, while
another marker was positioned at 23.8 cM below the gene. Microsatellite analysis on elite rice varieties with different genetic
background showed that all known sources of blast resistance included in this study carry the specific Pi-1(t) allele. Results are discussed considering the potential utility of the microsatellite markers found, for MAS in rice breeding
programs aiming at developing rice varieties with durable blast resistance based on a combination of resistance genes.
Centro Internactional de Agricultura Tropical (CIAT) institute where the research was carried out 相似文献
18.
A doubled haploid barley (Hordeum vulgare L.) population from a cross between the cultivar `Ingrid' and the Ethiopian landrace `Abyssinian' was mapped by AFLP, RFLP,
SSR and STS markers and tested for resistance to isolates`4004', `2', `16-6', `17', `22' and `WRS 1872' of Rhynchosporium secalis (Oudem.) J.J. Davis, the causal agent of leaf scald. Resistance tests were conducted on parents, DH-lines, a near-isogenic
line of `Abyssinian' (NIL) into `Ingrid', and an F2 population descended from the same F1 plants as the DHs. The DH population segregated for at least two major R. secalis resistance QTL. All isolates tested identified a major QTL on chromosome 3 (3H) associated with R. secalis resistance, in a 4 cM support interval between the co-segregating markers Bmac0209/Falc666 and MWG680. The QTL was linked
with the markers Falc666 (2.3 cM), YLM/ylp (0.3 cM), MWG680 (1.7 cM), cttaca2 (2.5 cM) and agtc17 (9.8 cM). The second QTL
was located on chromosome 1 (7H).However, this QTL was only detected by one isolate and was located in an interval of 16 cM
in the distal part of the chromosome. At this QTL the allele for improved scald resistance originated from the parent `Ingrid'.
There were a number of minor QTL on chromosomes 2 (2H), 4 (4H) and 6 (6H) that were not repeatable either across replications
or analysis methods. The importance of checking QTL-models by cross-validation is stressed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
19.
Early spontaneous diploidization of maternal maize haploids generated by in vivo haploid induction 总被引:2,自引:0,他引:2
The production of doubled haploid (DH) lines has become a key technology in maize (Zea mays L.) research and breeding. However, most of the haploid plants are sterile and in many cases artificial chromosome doubling involves the use of costly and toxic chemicals. Here, we report a special kind of doubled haploid named the early doubled haploid (EH) that was generated directly by in vivo haploid induction. We found 83 EH plants induced from the hybrid Zhengdan958, 55 families of its F2:3 population and the parental lines, all of which were confirmed to be homozygous diploids via flow cytometry and 104 SSR markers. The progeny of EH0 (EH1) behaved in the same manner and showed the same potentialities as the parents of Zheng58 and Chang7-2. EH plants were also detected in other genetic backgrounds at a frequency of 1–3.5 % based on the total number of haploid plants. Because the EH lines exhibited completely fertility and were obtained from induction directly in one step, they could be used in DH breeding as a new breeding strategy. According to our observations, it is likely that spontaneous doubling in EH occurred during embryo development when haploid induction. The possible mechanism of EH is also discussed. 相似文献
20.
Segregation for isozymes and HMW glutenins in a doubled-haploid cross of winter wheat carrying 1BL.1RS and 5DL.5RS translocations from rye 总被引:1,自引:0,他引:1
The doubled haploid (DH) wheat line ‘dh 5841’ carrying two translocations from rye, 5DL.5RS and 1BL.1RS, has been crossed to the subline of wheat cultivar ‘Amadeus 7143’ with a 1BL.1RS translocation. The resulting F1 hybrid IJ 98 with a heterozygous 5DL.5DS‐5DL.5RS chromosome pair has been used to produce doubled haploids. A total of 57 DH lines were obtained from plantlets regenerated in anther culture after successful colchicine treatment and seed set. These lines were identified regarding the constitution of chromosome 5D (5DL.5DS or 5DL.5RS) by means of isoenzyme marker analysis. Thirty DH lines possessed the 5DL.5DS chromosome, while the remaining 27 lines carried the 5DL.5RS translocation. For some of these lines, the 5DL.5RS chromosome was cytologically confirmed by C‐banding. Furthermore, the DH lines were evaluated for their high molecular weight glutenin subunit composition. All possible combinations for the four independent loci —Skdh, Glu‐Al, Glu‐B1 and Glu‐D1— were detected in only 57 DH lines and no segregation distortion was observed. 相似文献