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1.
L. L. Sanford K. L. Deahl S. L. Sinden R. S. Kobayashi 《American Journal of Potato Research》1995,72(5):261-271
The glycoalkaloid contents of tubers were measured in hybrid and back-cross populations derived from a cross ofSolarium chacoense Bitter (PI 472810, 2n=2x=24) withSolanum tuberosum L. (2n=4x=48). In tubers sampled from near the median size in each population and parent, the mean total glycoalkaloid (TGA) contents of theS. chacoense andS. tuberosum parent genotypes, and the F2 and F4 hybrid genotypes were 245, 4, 39, and 35 mg/ 100 g fresh weight (fw), respectively. Backcrossing the F2 genotypes toS. tuberosum reduced the mean TGA content to 15 mg/100 g fw. A second backcross further reduced the content to 9 mg/100 g fw. However, some genotypes in both backcross populations (25% and 10%) had TGA contents above the generally accepted upper limit of 20 mg/100 g fw. Tuber size was significantly smaller in theS. chacoense parent and the hybrid populations compared with the size in theS. tuberosum parent and backcross populations. In a second sampling of tubers from each population, intentionally selected to equalize tuber size among populations, the mean tuber size for populations was reduced by 79%. The mean population TGA content increased by 44%. However, the differences and rankings among parent, hybrid, and backcross populations were the same for both tuber samplings. 相似文献
2.
L. L. Sanford S. P. Kowalski C. M. Ronning K. L. Deahl 《American Journal of Potato Research》1998,75(4):167-172
The leptine glycoalkaloids fromSolanum chacoense Bitter are potential resistance factors to Colorado potato beetle,Leptinotarsa decemlineata Say. A selected sample of 17 F1 hybrids (4x) from crosses of a 4xS. chacoense clone that synthesizes leptines (8380-1) with 4xS. tuberosum L. lines were selfed to generate F2 hybrids (4x) and were also backcrossed toS. tuberosum. Glycoalkaloid (GA) content in foliage and tubers was measured for 786 sibling genotypes from the two generations in the field at Beltsville, Maryland. Leptines were found in the foliage of all F2 hybrids and in 98% of the backcross genotypes. Leptines were not detected in tubers from either generation. Foliage concentrations of leptines for the F2 hybrids averaged 156 mg/100g fresh weight (FW). The proportion of the total GA content consisting of leptines averaged 42%. By backcrossing the F1 hybrids toS. tuberosum the average concentration of leptines was reduced to 44 mg/100g FW. The proportion of leptines averaged 16%. The tuber contents of solanine plus chaconine glycoalkaloids averaged 52 mg/100g FW for the F2 hybrids and 27 mg/100g for the backcross genotypes. 相似文献
3.
ASolanum tuberosum L. Gp. Tuberosum population was recurrently selected for resistance to potato leafhopper (PLH) (Empoasca fabae, Harris). Primary selection was for low levels of nymphal infestation under natural infestation in field tests. The selection procedures allowed completion of a selection generation per year. The original population and seven selection-generation populations were tested for PLH resistance for three years at two locations. Averaged over six tests, seven generations of selection reduced the level of nymphal infestation by 71% and severity of plant damage (hopperburn) by 45%. The rate of reduction slowed in later generations. 相似文献
4.
J. J. H. M. Allefs W. Van Dooijeweert E. R. De Jong W. Prummel J. Hoogendoorn 《Potato Research》1995,38(1):11-21
Summary Eleven clones obtained from a cross between cv. Katahdin and fusion products betweenSolanum tuberosum and the non tuber-bearing speciesS. brevidens, were backcrossed again withS. tuberosum (clone AR80-127-5). Small tubers harvested from 583 seedlings of these second backcross (BC2) populations were screened for
tuber tissue resistance toErwinia carotovora subsp.atroseptica under aerobic conditions. After multiplication in the field. BC2 clones again were screened for soft rot resistance under
aerobic as well as anaerobic conditions. In general, the resistance of the BC2 populations was reduced in comparison with
the BC1. Variation for resistance was found within the BC2 populations, but there was no correlation between the results of
the three tests performed. Four BC2 populations were also screenned for resistance to blackleg in the field. Significant differences
were found between populations for mean percentage of diseased plants, but these differences could not be explained by the
resistance of the parental clones. 相似文献
5.
The glycoalkaloid contents of foliage were measured in populations derived from a cross ofSolanum chacoense Bitter (PI 472810) with a composite sample of genotypes from a randomly matedSolanum tuberosum L. population. The mean total glycoalkaloid contents of the parent PI 472810 andtuberosum populations, and the F2 and F4 populations were 856, 121, 286, and 279 mg/100g fresh weight, respectively. The total glycoalkaloid content of the first backcross population, F2 xtuberosum, was 290 mg/100 g, close to the content of the F2. In a second backcross totuberosum, the mean total content was 148 mg/100 g, close to the content of the parenttuberosum population. Only the glycoalkaloids α-solanine and α-chaconine were found in PI 472810, whereas in the parenttuberosum, hybrid, and backcross populations, the glycoalkaloids α & β-solamarine also were found in a small number of genotypes. The ratios of chaconine to solanine contents were significantly (P≤.05) different in the parent PI 472810 andtuberosum populations, 2.55 and 2.12, respectively. The ratios in the F2 and F4 populations were similar to that of PI 472810, whereas the ratios in the backcross populations were closer to that of thetuberosum parent. The levels of foliage glycoalkaloids in the hybrid and backcross populations paralleled the levels of potato leafhopper,Empoasca fabae Harris, resistance measured in a previous study. 相似文献
6.
We identified clones inSolanum tuberosum L. gp. Tuberosum breeding populations that, although susceptible to infestation by potato leafhopper,Empoasca fabae (Harris), exhibited a tolerance which was manifested by the ability to produce higher than expected tuber yields when heavily infested with leafhoppers. Such tolerance was noted in a number of clones in varying degrees, but one, B6930-1, was exceptional. It sustained only a 19% loss in tuber yield compared to ‘Katahdin,’ a susceptible cultivar, that averaged 33% lower tuber yields under similar infestation levels. We studied the seasonal growth patterns of this clone and compared them to those of Katahdin, and B6705-10, a breeding clone with known resistance to leafhopper infestation. We found that much of B6930-l’s tolerance results from its early, vigorous vine growth and its early and rapid rate of tuber bulking, enabling this clone to escape some of the effects of leafhopper feeding, whereas Katahdin was usually just beginning to tuberize at the height of leafhopper infestation. 相似文献
7.
《Field Crops Research》1988,19(3):183-200
Nine experiments were run at three hot tropical sites (5–12°S, 180–800 m) within Peru to quantify the influence of plant population on soil temperature and growth and yield of the potato.Radiation interception was greatest at the highest plant populations and soil cooling was directly proportional to the amount of crop cover over the soil, but no appreciable effect on the timing of tuber initiation was apparent. More stems per unit land area leading to a higher leaf area index (lai) were primarily responsible for greater interception of radiation at the higher plant populations, although some compensation in stem number per unit land area and in lai at lower populations was evident later in the season.In general, tuber yield increased linearly with increases in planted population over the range studied (2.7–12.5 plants m−2, and in one experiment to 31.7 plants m−2), and was proportional to increases in the amount of intercepted radiation. Tuber yields ranged from 8 to 60 t ha−1 over sites and populations. Vigorous clones with Solanum tuberosum spp. andigena in their genetic background constituted the exceptions to this linear trend, and for these clones yields declined at the highest populations, particularly when the rectangularity of planting vastly deviated from square patterns. Tuber yield of Solanum tuberosum spp. tuberosum and Neotuberosum (S. tuberosum spp. andigena selected for tuberization under long-day conditions) clones did not respond to variations in rectangularity of planting and, probably due to their small stature and early maturity, did not demonstrate signs of intense between-plant competition for tuber yield as measured with the Kira competition density index. In contrast, for clones with Solanum tuberosum ssp. andigena in their genetic background, maximum tuber yield at populations greater than 5.5 plants m−2 was dependent on the rectangularity of planting, and declined as the latter deviated from squareness.Since the proportion of marketable tubers was scarcely affected by the planting densities, plant population of S. tuberosum ssp. tuberosum clones planted in hot climates should be as close as possible without limiting the amount of soil available for hilling-up. 相似文献
8.
L. L. Sanford K. L. Deahl S. L. Sinden T. L. Ladd 《American Journal of Potato Research》1992,69(11):693-703
Solanum tuberosum L. Gp. Tuberosum populations that had been improved for resistance to potato leafhopper,Empoasca fabae (Harris), by recurrent selection, were analyzed for tuber contents of the glycoalkaloids α-solanine and α-chaconine. The mean content of both glycoalkaloids was significantly higher in the more resistant populations compared with the unselected population. In a population generated from seven cycles of selection for resistance, the mean content of solanine + chaconine was 9.5 mg/100 g fresh weight compared with 4.0 mg/100 g in the original, unselected population; a 137% increase. Kennebec, in the same experiment, averaged 10.9 mg/100 g; Katahdin averaged 4.6 mg/100 g. 相似文献
9.
J. Mitchell McGrath Christie E. Williams Geraldine T. Haberlach Susan M. Wielgus Thomas F. Uchytil John P. Helgeson 《American Journal of Potato Research》2002,79(1):19-24
Resistance to potato tuber soft rot caused byErwinia carotovora was transferred fromSolanum brevidens to the cultivated potato over the course of four backcross generations originating from a somatic hybrid. Soft rot reactions were determined via a tuber plug inoculation method developed during the course of these experiments. Soft rot resistance was highest in the somatic hybrid (only ca. 20% of tubers and plugs showed evidence of severe rotting) and lowest among progeny of control potato x potato crosses (ca. 80% of tuber plugs showed severe rot). Backcross generations involving somatic hybrids were intermediate in their reaction, and resistance stabilized to about 60% of tuber plugs showing severe rot in the BC2 through the BC4. Reciprocal crosses showed no difference in the inheritance of soft rot resistance, indicating that neitherS. brevidens norS. tuberosum donor cytoplasm had a significant effect on the expression of resistance. Crosses between BC3 siblings where noS. brevidens genetic markers were detected but resistance was segregating demonstrated a dosage effect for soft rot resistance. We conclude that introgression of soft rot resistance has occurred and that at least one locus responsible for resistance inS. brevidens now resides in theS. tuberosum genome. 相似文献
10.
Segregation of leptines and other glycoalkaloids inSolanum tuberosum (4x) ×S. chacoense (4x) crosses
L. L. Sanford R. S. Kobayashi K. L. Deahl S. L. Sinden 《American Journal of Potato Research》1996,73(1):21-33
A clone, 8380-1, selected fromSolanum chacoense (PI 458310) for its high foliage content of the leptine glycoalkaloids, a factor in resistance to Colorado potato beetle, was doubled in chromosome number from 2n=2x=24 to 2n=4x=48. Three 4x clones were crossed with sixS. tuberosum (4x) clones. Foliage glycoalkaloid contents were measured for 452 F1 hybrids from 15 crosses. The 4xchacoense parental clones were not different in respect to glycoalkaloid contents and were similar to the original 2x clone. All F1 hybrids synthesized foliage leptines ranging from 9 to 369 mg/100 g fresh weight (fw) with a mean content of 113 mg/100 g fw. The proportion of leptines in the total glycoalkaloid content ranged from 1% to 62% with a mean of 25%. The 4xchacoense parent mean leptine content was 1482 mg/ 100 g fw which was 90% of the total glycoalkaloid content. Tubers from 136 hybrids and the threechacoense parental clones were tested for glycoalkaloid contents. The tuber solanine + chaconine contents of the 136 hybrids ranged from 30 to 180 mg/100 g fw with a mean of 79. The mean tuber content of the threechacoense parental clones was 157 mg/100 g fw. Leptines were not found in any of the tubers. 相似文献
11.
Summary The resistance to tuber soft rot caused byErwinia carotovora ofSolanum tuberosum x S. tarijense andS. tuberosum (+) S. commersonii hybrids and their backcrosses is reported. A number of resistant diploid sexual hybrids and tetraploid/hexaploid somatic
hybrids were selected. Backcross progenies were obtained through 2x×4x crosses involving a resistant diploid hybrid and tetraploidS. tuberosum, and through 4x×4x crosses between a resistant somatic hybrid andS. tuberosum. The hybrids showed high variability interms of resistance to tuber soft rot. The resistance of progeny from 2x×4x backcrosses
was similar to that of the parental sexual hybrid. By contrast, the resistance of genotypes deriving from 4x×4x backcrosses
was reduced compared with the resistant somatic hybrid. In general, tuber characteristics of the backcross hybrids improved
considerably as compared with their parents, and tuber yield per plant was good. 相似文献
12.
Summary Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploid S.tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out
of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics,
chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two
parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied
considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights
than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV. 相似文献
13.
Solanum tuberosum Group Tuberosum plants grown in a non-nutritive media exhibit a shoot inhibition which follows the normal tuber bud dormancy. The shoot inhibition is characterized by very slow growth of the aerial portion of the plant with little leaf development. An external source of nitrogen, either nitrate-N or ammonium-N, applied to the roots of Iopride plants overcame the inhibition while only a nitrate-N source was effective in overcoming the inhibition of Kennebec plants. Group Phureja and Stenotomum and otherSolanum species evaluated did not show the shoot inhibition phenomenon. 相似文献
14.
M. J. De Maine C. P. Carroll H. E. Stewart R. M. Solomon R. L. Wastie 《Potato Research》1993,36(1):21-28
Summary DiploidSolanum phureja, diploid and tetraploidS. tuberosum × S. phureja hybrid, and tetraploidS. tuberosum backcross clones were assessed for resistance to commercially important diseases. There was a reduction in the expression
ofS. phureja characteristics with increasing dosage ofS. tuberosum genetic material. The generally high resistance of theS. phureja group to common scab, potato leafroll virus and potato virus Y decreased, while resistance to gangrene, foliage and tuber
blight tended to increase.
Resistance genes were probably well dispersed throughout theS. phureja genotype so that many were lost on hybridisation, butS. phureja could be a useful source of scab and virus resistance in the production ofS. tuberosum cultivars. 相似文献
15.
H. W. Platt 《American Journal of Potato Research》1992,69(3):179-186
Progeny tubers from seed potatoes originating from either a traditional clonal selection method or the more modernin vitro tissue culture method of propagation were tested for storage rot response following inoculation of three tuber sites with twoFusarium species. Significant differences were found among disease responses for the twoFusarium species and for the four cultivars tested. Disease symptoms were less severe forF. solani var.coeruleum than forF. sambucinum. Fusarium sambucinum caused less disease in Superior than Kennebec, Russet Burbank and Sebago. Seed propagation method did not significantly affect disease response except for one case;in vitro culture of Kennebec had less disease due toF. sambucinum at the tuber side inoculation site than clonal selection. Kennebec and Superior inoculatedF. sambucinum had significant differences with respect to the number of years of field propagation for the tuber side inoculation site and for the tuber mean rot index. Significant differences in number of years of field propagation were also found for eye-ends of Superior tubers and stem ends of Russet Burbank tubers inoculated withF. solani var.coeruleum. 相似文献
16.
Piara S. Bains Vikram S. Bisht Dermot R. Lynch Lawrence M. Kawchuk John P. Helgeson 《American Journal of Potato Research》1999,76(3):137-141
Development of potato cultivars resistant to Erwinia species (Erwinia carotovora subspeciesatroseptica, E. carotovora subspeciescarotovora, andErwinia chrysanthemi) causing soft rot of stems and tubers involves identification and transfer of the resistance to breeding lines possessing desirable agronomic traits. In total, 363 accessions of six wildSolanum species, three somatic hybrids and nine sexual progeny of somatic hybrids were screened for stem soft rot resistance. Thirty-one, 50, and 67 percent of accessions ofSolanum boliviense, Solanum chacoense, and Solanumsanctarosae, respectively, were resistant or highly resistant, whereas, 100, 97, and 95 percent of accessions ofSolanum canasense, Solanum tarijense, andSolanum spegazzinii were susceptible or highly susceptible, respectively. Two tuber soft-rot resistant somatic hybrids, A937 and T355-11 produced earlier by the fusion ofSolanum brevidens (PI 218228) andSolanum tuberosum (PI203900) orS. tuberosum cv. Russet Burbank, respectively, and the sexual progeny of A937 andS. tuberosum cv. Katahdin were also highly resistant. Transfer of resistance fromS. brevidens to somatic hybrid (A937) and subsequently to the progeny suggests that the resistance is simply inherited. In addition to the identification of 65 resistant or highly resistant clones of wildSolanum species, this study has identified three tuber soft rot-resistant clones that are also resistant to stem soft rot. Potato cultivars showed a range of responses to the pathogen. 相似文献
17.
Domenico Carputo Luigi Frusciante Luigi Monti Mario Parisi Amalia Barone 《American Journal of Potato Research》2002,79(5):345-352
Tetraploid BC3 hybrids between sexually incompatible speciesSolanum commersonii andS. tuberosum were characterized for tuber quality (stolon length, tuber eye depth, chipping color from 3-month storage at 5 C, and specific gravity) and soft rot resistance traits. For all analysed morphological traits, except stolon length, genotypes resembled theS. tuberosum phenotype. On average, the specific gravity of selected genotypes was quite high (1.087), and 12 of them had a value >1.090. As for chipprocessing ability, 4.8% of genotypes chipped well directly from cold storage, and 14.3% after reconditioning. All but one of these genotypes also possessed a high specific gravity of tubers. Variability was found for resistance toErwinia carotovora subsp.carotovora. In particular, 24% of hybrids had a rotted area diameter ≤ 4 mm and were classified as resistant. The average percentage ofcommersonii-specific markers still present in the BC3 hybrids was 22%, ranging from 9% to 37%. Genotypes combining good qualitative tuber traits with a wild genome content lower than the average value of the population were selected for further breeding efforts. The importance of molecular-assisted selection is discussed. 相似文献
18.
The productive potential of potatoes (Solanum tuberosum L. cvs. Norland, Superior, Norchip, and Kennebec) was assessed for life support systems being proposed for space stations and/or lunar colonies. Plants were grown in walk-in growth rooms for 15 weeks at 20 C under 12-, 16- and 20-h photoperiods of 400 μmol mt-2st-1 photosynthetic photon flux (PPF). Norland yielded the greatest tuber fresh weight, producing 2.3, 2.4, and 2.9 kg/plant under 12-, 16-, and 20-h photoperiods, respectively. The respective yields for the other cultivars under 12-, 16-, and 20-h were: Superior, 1.9, 1.5, and 1.8 kg/plant; Norchip, 1.8, 1.4, and 2.0 kg/plant; and Kennebec, 2.3, 0.2, and 0.8 kg/plant. Shoot and total plant biomass increased with lengthening photoperiods except for Kennebec, which showed increased shoot growth but no change in total growth with the longer photoperiods. Kennebec shoot growth under the 20-h photoperiod, and to some extent under 16-h, was noticeably stunted with shortened internodes. In addition, leaves of these plants showed mild chlorosis with rusty “flecking” of the surfaces. The harvest index (ratio of tuber yield/total biomass) was highest for all cultivars under the 12-h photoperiod, with a maximum of 0.69 for Norland. Similarly, the tuber yield per input of irradiant energy also was highest under 12-h for all cultivars. The tuber yield expressed on an area basis for the highest yielding treatment (Norland under 20-h) equaled 2.2 kg dry matter mt-2. Over 15 weeks this equates to a productivity of 20.7 g tuber dry matter mt-2 dayt-1. Assuming 3.73 kcal per g tuber dry matter and a daily human dietary requirement of 2800 kcal, then 36 m2 of potatoes could supply the daily energy requirement for one human. Potential for increasing productivity is discussed. 相似文献
19.
Veli-Matti Rokka Jari P. T. Valkonen Airi Tauriainen Leena Pietilä Renata Lebecka Ewa Zimnoch-Guzowska Eija Pehu 《American Journal of Potato Research》2000,77(3):149-159
Protoplasts were fused to produce somatic hybrids between a triploid (2n=3x=32-34) interspecific somatohaploid betweenSolanum brevidens Phil. andS. tuberosum L., and a dihaploid (2n=2x=24) anther-derived line ofS. tuberosum cv. Van Gogh. A total of 265 plants were regenerated from protoplast fusion derived calli and their hybridity was verified using fusion partner specific RAPD markers. These “second generation” somatic hybrids were aneuploid pentaploids (2n=5x=51-65) with a 2C DNA content ranging from 3.36 to 4.43 pg, which corresponded to the sum of the 2C values of each of the fusion partners (somatohaploid: 2.22 pg; and the dihaploid line of cv. Van Gogh: 1.87 pg). Most of the “second generation” somatic hybrids were vigorous, but variable in morphology. They were extremely resistant to PLRV and they had tolerance to PVY infection derived from the somatohaploid fusion partner. Even though most of the “second generation” hybrids tuberized, the tuber morphology was variable and most were poorly shaped. InErwinia soft rot resistance tests, the tubers showed higher level of resistance than the tetraploidS. tuberosum cultivars, the dihaploidS. tuberosum fusion partners and the hexaploid somatic hybrids betweenS. brevidens andS. tuberosum. The “second generation” somatic hybrids were all male sterile and failed to produce berries or seeds. 相似文献
20.
Resistance to blackleg and tuber soft rot in sexual and somatic interspecific hybrids with different genetic background 总被引:1,自引:0,他引:1
Domenico Carputo Teodoro Cardi Mario Speggiorin Astolfo Zoina Luigi Frusciante 《American Journal of Potato Research》1997,74(3):161-172
Diploid interspecific hybrids from crosses betweenSolanum tuberosum haploids andS. canasense, S. multidissectum, andS. tarijense, along with tetraploid and hexaploid somatic hybrids S.tuberosum (+)S. commersonii, were screened for resistance to blackleg and tuber soft rot byErwinia carotovora subsp.atroseptica andE. carotovora subsp.carotovora. Among the diploid hybrids, those involving clone mlt la of S.multidissectum and tar 2b ofS. tarijense yielded the highest number of genotypes resistant to both blackleg and tuber soft rot. By contrast, all the hybrids involving clone tar 1lb ofS. tarijense were susceptible to both bacterial diseases. As far as resistance of somatic hybrids is concerned, the most interesting genotype was the tetraploid one which showed resistance to both blackleg and tuber soft rot Significant correlations were found betweenE. carotovora subsp.atroseptica andE. carotovora subsp.carotovora both in resistance to blackleg and in resistance to tuber soft rot, suggesting that resistance to the two subspecies is controlled by the same gene/s or that the relevant genes are linked. On the other hand, correlations between blackleg and tuber soft rot were never significant, indicating that different mechanisms may control resistance in tuber and stems. Alternatively, the resistance could be controlled by the same gene/s under a different spatial/temporal expression pattern. Twelve diploid sexual hybrids and one tetraploid somatic hybrid were selected for resistance to blackleg and/or tuber soft rot and for tuber characteristics. 相似文献