Cultivar response to Fusarium storage rot as affected by two methods of seed origin propagation; Clonal selection andin vitro culture

Progeny tubers from seed potatoes originating from either a traditional clonal selection method or the more modernin vitro tissue culture method of propagation were tested for storage rot response following inoculation of three tuber sites with twoFusarium species. Significant differences were found among disease responses for the twoFusarium species and for the four cultivars tested. Disease symptoms were less severe forF. solani var.coeruleum than forF. sambucinum. Fusarium sambucinum caused less disease in Superior than Kennebec, Russet Burbank and Sebago. Seed propagation method did not significantly affect disease response except for one case;in vitro culture of Kennebec had less disease due toF. sambucinum at the tuber side inoculation site than clonal selection. Kennebec and Superior inoculatedF. sambucinum had significant differences with respect to the number of years of field propagation for the tuber side inoculation site and for the tuber mean rot index. Significant differences in number of years of field propagation were also found for eye-ends of Superior tubers and stem ends of Russet Burbank tubers inoculated withF. solani var.coeruleum.     

Effect of daminozide on survival,growth and yield of micropropagated potatoes

The effect of daminozide [Butanedioic acid mono (2,2-dimethyl hydrazide)] on growth, field establishment and productivity of micropropagated Kennebec and Russet Burbank potato was studied. Addition of daminozide to the medium significantly reduced stem length ofin vitro propagated plantlets. Optimal dose was 0.6 mg active ingredient per plant. This dose did not significantly alter the number of nodes and leaf area. Three transplanting methods were examined: direct from tissue culture to the field without protection; the same but with a plastic covering for protection; and after 7 days acclimation in a greenhouse but without protection in the field. Stand establishment ranged from 83.3 to 100%. The second transplanting method had the highest percentage establishment for both cultivars. The application of daminozide improved survival for all transplants of Kennebec and the directly planted plantlets of Russet Burbank. Independent of the daminozide treatment, plants of both cultivars hardened in the greenhouse prior to transplanting produced the highest number and yield of tubers. For Russet Burbank, daminozide caused a delay in tuberization but did not affect tuber bulking and haulm growth.     

Potato leafroll virus spread in differentially resistant potato cultivars under varying aphid densities

An action threshold of 3-10 green peach aphid,Myzus persicae (Sulzer), apterae per 100 lower leaves is recommended for use in Minnesota to prevent further spread of potato leafroll virus (PLRV) in potato,Solarium tuberosum L. This threshold was first developed and validated using the PLRV susceptible cultivar Russet Burbank. Here we report experiments to determine if higher aphid densities could be tolerated in PLRV resistant cultivars,i.e., Kennebec (moderately resistant) or Cascade (highly resistant), without an increase in PLRV infection. Insecticidal sprays were applied to plots when predetermined target aphid densities, based on number of apterae per 100 leaves, were reached: 3, 10, 30, and 100 (Russet Burbank); 10, 30, 100, and 300 (Kennebec); and 30, 100, 300, and 1000 (Cascade). The response variable was the average percentage of PLRV infected plants. Overall mean cumulative aphid-days and percent PLRV infection were 617 and 23.5% for Russet Burbank, 1,296 and 10.2% for Kennebec, and 4,816 and 9.5% for Cascade. For each cultivar, the highest target aphid density tolerated without an increase in PLRV spread was determined by comparing PLRV infection in plots sprayed on predetermined thresholds to PLRV infection in plots where aphids were rigorously controlled. This maximum density was 10 apterae per 100 leaves for Russet Burbank and 300 apterae per 100 leaves for Cascade. Results using Kennebec were ambiguous, but Kennebec was always more resistant to PLRV than Russet Burbank. Excised leaflet tests showed that the cultivars did not differ in resistance to green peach aphid. It appears that action thresholds based on green peach aphid apterae can be different depending upon the inherent PLRV-resistance of the cultivar.     

Application of a PCR-based test for detection of potato late blight and pink rot in tubers

A polymerase chain reaction (PCR)-based test for potato late blight (Phytophthora infestans) and pink rot (P.erythroseptica, P. nicotianae) diseases has been developed for use with potato tuber tissue. Primers based on sequence analysis of the ITS2 region of ribosomal DNA of late blight and pink rot pathogens were utilized in PCR assays of inoculated tubers and tubers harvested from plots known to have late blight and/or pink rot. Assays of artificially inoculated Kennebec and Russet Burbank tubers revealed thatP. infestans was detected by PCR as early as 72 h after inoculation and in the absence of visible symptoms. Much higher detection frequencies were obtained by PCR compared with plating on selective medium or placement of tissue in moist chambers. Tubers from plots known to have late blight and/or pink rot were tested using the PCR assay. Assay of late blight lesions showed ca. 80% recovery for late blight-infected tubers from the field. Results indicate that the PCR assay provides a rapid and accurate test for diagnosis of late blight and pink rot in potato tubers.     

Effect of leaves on microtubers produced from potato single-node cuttingsIn Vitro

Microtubers are used to propagate, to store, and to transport potato clones. Culturing single-node explants from potato plantletsin vitro without subtending leaves was reported to result in plantlets with lower vigor and a higher coefficient of variation. The effect on microtuber productionin vitro of leaf area and the presence or absence of leaves on potato single-node cuttings was investigated as an extension of the above study. Stock plantlets of potato cvs Atlantic, Kennebec, Russet Burbank, and Shepody were cultured under a 16-h photoperiod. Single-node cuttings were excised and grown in a high-sucrose tuberization medium in darkness. Leaf area did not affect the frequency, size, or weight of microtubers of cvs Katahdin and Russet Burbank. The absence of leaves reduced microtuber diameter for Russet Burbank; whereas Atlantic, Kennebec, and Shepody were unaffected. Mean fresh weight of microtubers was reduced when leaves were removed for all cvs except Atlantic. No effect of the removal of the leaf was observed for mean dry weights of microtubers from all cvs, although microtubers from single-node cuttings without leaves accumulated significantly more percent dry matter than those with leaves. Rapid multiplication facilities may therefore wish to consider conserving resources such as media, vessels, and growth room space by culturing explants without leaves for the production of microtubers.     

Translocation and mechanical spread of a Minnesota isolate of potato virus S in potatoes

Potato cultivars Norland, Kennebec, and Russet Burbank were inoculated with a Minnesota isolate of potato virus S (PVS) in the field. The incidence of secondary infection, measured by enzyme-linked immunosorbent assay (ELISA), indicated that significantly more PVS infection occurred in Russet Burbank than Norland or Kennebec, and that Norland had significantly more infection than Kennebec (P≤ 0.01). Mature plant resistance was not observed. Rapid reinfection of PVS-free seedlots resulted from inoculum point-sources when routine cultivation practices were followed. Reinfection rates were significantly greater for seedlots grown at Grand Forks, ND, when compared with seedlots grown at Becker, MN, during both years of the study (P≤ 0.01). After two years in the field, reinfection rates for Norland (71.8%) and Russet Burbank (73.0%) did not differ significantly, but were significantly greater than the reinfection rate for Kennebec (29.5%) (P≤ 0.01). In greenhouse-grown plants, PVS moved out of rub-inoculated leaves within 24 hr, however, 13 and 20 days were required before PVS was detected with ELISA in foliage above and below the inoculated leaf, respectively. The frequency of PVS detection was significantly greater in foliage above the inoculated leaf compared to foliage below the inoculated leaf (P≤ 0.05). Translocation of PVS from inoculated leaves to daughter tubers occurred within 13 days for Russet Burbank and Norland and within 20 days for Kennebec.     

The transmission of Potato Virus S by the cutting knife and retention time of infectious PVS on common surfaces

The transmission of PVS by the cutting knife in potato cvs Norland, Kennebec and Russet Burbank was studied in the field and greenhouse. Field studies showed transmission to Norland via tuber infection was significantly less than for Kennebec and Russet Burbank (P = 0.01). However, significant cultivar differences were not found in the greenhouse (P = 0.05). Contamination of the cutting knife by cutting through sprouts resulted in significantly greater numbers of plants infected for cv Kennebec than when sprout contact was avoided (P = 0.05). Although the same trends were observed for cv Norland and Russet Burbank the differences were not significant (P = 0.05). Infectious PVS particles were readily recovered from materials commonly contacted during potato cultivation, storage and processing. Retention time of infectious PVS was usually greater when materials were held at 4°C and 100% relative humidity than at ambient greenhouse conditions. Retention times ranged from 0 hr (PVS not recovered) to 180 hr on unpainted wood. Results demonstrated the need for strict indexing and sanitation procedures during the production of PVS-free potato seed.     

Ac brador: a new late-maturing cultivar with a high degree of field resistance to late blight

AC Brador is a late maturing yellow-fleshed cultivar. The vine maturity is slightly later than Russet Burbank. The oblong tubers have a specific gravity which is slightly higher than Kennebec. The boil and bake quality of AC Brador is similar to Kennebec. The mean French fry quality of AC Brador is below that of Russet Burbank but still in the acceptable range. This cultivar has extremely high field resistance to late blight resistance to verticillium wilt and early blight, a relatively high resistance to PLRV, and a moderate resistance to PVYand rhizoctonia.     

Effects of sulphur dioxide and nitrogen dioxide on fourSolanum tuberosum L. cultivars

The effects of sulphur dioxide (SO₂) and nitrogen dioxide (NO₂), singly and in combination, on the shoot growth of four potato cultivars differing in maturity classification (Solanum tuberosum L. ‘Superior,’ ‘Norchip,’ ‘Kennebec, rs ‘Russet Burbank’) were determined in controlled conditions. Plants were exposed to 0.11 ppm SO₂ and/or 0.11 ppm NO₂ for 24 hours a day for 7 or 14 days. There were no significant differences in leaf growth of ‘Superior’ or ‘Norchip’ plants at 7 or 14 days. Stem dry weight was significantly reduced in ‘Superior’ only after 14 days in the mixture. ‘Kennebec’ and ‘Russet Burbank’ plants had significantly less leaf area, leaf fresh weight, and leaf water content after 14 days of exposure to the mixture, but there was no significant change in dry weight. Specific leaf weight was significantly increased in the mixture treated plants of both cultivars. The two earlier maturing cultivars, ‘Superior’ and ‘Norchip,’ were less sensitive to the SO₂ and NO₂ combination than the two later maturing cultivars, ‘Kennebec’ and ‘Russet Burbank’.     

Wound-healing and the effect of soil temperature,cultivars and protective chemicals on wound-healed potato seed pieces inoculated with seed piece decay fungi and bacteria

The effect ofFusarium solani (Fsc),F. sambucinum (Fs),Erwinia carotovora subsp.atroseptica (Eca), andE. carotovora subsp.carotovora (Ecc) inoculated singly and in combination (FscFs, FscEca, FscEcc, FsEca, FsEcc, and EcaEcc) on wound-healed potato seed pieces of the cultivar Kennebec was studied. Potato seed pieces wound-healed for 5 days at 13 C and approximately 100% relative humidity, inoculated with Fsc, Fs, Eca, Ecc and their combinations, and incubated for 10 days at 9 C were protected from Eca, Ecc, and EcaEcc. When wound-healed potato seed pieces were inoculated with Fsc, Fs, Eca, Ecc, and their combinations and planted in soil maintained at 7, 10, 15, 20 and 25 C, wound-healing protected seed pieces from all pathogens and their combinations in seed pieces held for 10 days at 10 and 15 C. When seed pieces of cultivars Atlantic, Kennebec, Norchip, Russet Burbank and Superior were inoculated with Fsc, Fs, Eca, Ecc, and their combinations, different levels of protection were observed: Russet Burbank had the highest level of protection, Atlantic and Kennebec were intermediate, and Norchip and Superior were slightly protected. Dusting mancozeb on wound-healed seed pieces before inoculation with Fsc, Fs, Eca, Ecc, and their combinations increased protection against all pathogens and their combinations by 39% or more compared to the nonchemical control.     

Wallowa Russet: A full season long Russet for processing and fresh market

The Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture jointly released Wallowa Russet in 2002. This late maturing variety is most suitable for frozen french fry use, but may find limited fresh market potential because of outstanding flavor and texture when baked. Wallowa Russet was evaluated as AO87277-6 in Oregon irrigated trials from 1993 through 1999, and in western regional trials from 1997 through 1999. Averaged over 13 locations in seven states, Wallowa Russet produced total yields of 55.6 mt/ha compared to 53.5 and 45.7 mt/ha for Russet Burbank and Russet Norkotah, respectively, and U.S. No.1 yields of 44.4 mt/ha compared to 30.7 and 36.0 mt/ha for Russet Burbank and Russet Norkotah. Specific gravity is consistently higher for Walowa Russet than for Russet Burbank and fry color is as light or lighter; sugar end development is less than that observed in Russet Burbank. Wallowa Russet is similar to Russet Burbank in susceptibility toVerticillium wilt, foliar early blight (Alternaria solani), and potato leafroll virus (PLRV). However, Wallowa Russet develops less net necrosis than Russet Burbank from PLRV infection. Wallowa Russet is more susceptible to common scab (Streptomyces scabies) than Russet Burbank. It has moderate resistance to potato virus Y (PVY). Tubers of Wallowa Russet are less susceptible to tuber infection and decay caused by potato late blight (Phytophthora infestans) than Russet Norkotah and Ranger Russet. Wallowa Russet has less hollow heart, brown center, blackspot bruise, and fewer growth cracks than Russet Burbank but is more susceptible to shatter bruise. Vitamin C is higher for Wallowa Russet (30.4 mg/100 g fresh weight) than for Russet Burbank (21.9) or Russet Norkotah (22.0), and protein content averaged 5.6% compared to 4.7% and 4.9% for Russet Burbank and Russet Norkotah, respectively.     

Use of jasmonate for conditioning of potato plantlets and microtubers in greenhouse production of minitubers

A two-year study was conducted to determine the effects of (1) jasmonic acid (JA) pre-treatment, (2) JA supplement in culture media, (3) cultivar (Amisk, Atlantic, Russet Burbank, Shepody, and Umatilla Russet), (4) light (0 h, 8 h), and (5) dormancy breaking treatment (Rindite, gibberellic acid) on greenhouse production of minitubers from microtubers andin vitro plantlets. The microtubers were produced under short day (8 h) light conditions and in darkness, from stock plantlets pre-treated with JA and untreated, and on tuberization media with or without JA.In vitro plantlets (the industry choice in nuclear seed potato production) of all five cultivars performed well, meeting the standard criteria for greenhouse production of minitubers. Production of minitubers from microtuber-derived plants of cvs Amisk, Russet Burbank, and Umatilla Russet was similar to that of plantlet-derived plants with regard to number of minitubers. Yields (weight), however, were lower than those from plantlets. Microtuber responses to JA varied with cultivar. Amisk produced the highest number of minitubers per plot from microtubers derived from JA pre-treated plantlets. Jasmonic acid-pretreated microtubers also gave significantly more minitubers in Russet Burbank and Umatilla Russet than the microtubers from other treatments. Shepody did not benefit from JA treatments and JA pre-treated Atlantic microtubers performed poorly, producing significantly lower yields of minitubers than other cultivars. Independently of cultivar, microtubers produced under 8-h photoperiod gave significantly higher yields of minitubers than microtubers produced in the dark. Dormancy release was the key factor influencing microtuber performance. Rindite proved to be a much more effective dormancy breaking treatment than gibberellin. JA conditioning of stock plants prior to tuberization is being proposed as a treatment in production of microtubers for greenhouse production of minitubers.     

Alturas: A multi-purpose, Russet potato cultivar with high yield and tuber specific gravity

‘Alturas’, a late-maturing, high-yielding, russet potato cultivar with high tuber specific gravity, was released in 2002 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington. Originally selected for dehydration processing, its cold-sweetening resistance also makes it suitable for processing out of storage into french fries and other frozen potato products. Culinary quality is high, with larger tubers suitable for fresh market if heavily russeted skin is not essential. Alturas consistently produced greater total and U.S. No. 1 yields than ‘Russet Burbank’ and ‘Ranger Russet’ in southern Idaho trials. Across other western trial sites, total yields of Alturas have on average been 29% and 14% greater than Russet Burbank and Ranger Russet, respectively. Alturas is resistant to Verticillium wilt (Verticillium dahliae) and early blight (Alternaria solani). Compared with Russet Burbank and Ranger Russet, Alturas is less susceptible to corky ringspot and foliar and tuber infection by late blight (Phytophthora infestans). It also is less susceptible to tuber net necrosis and Fusarium dry rot than Russet Burbank, and is more resistant to common scab (Streptomyces scabies) than Ranger Russet. However, Alturas is more susceptible to infection by PVY and PVX than Ranger Russet. Total nitrogen application recommendations for Alturas are approximately 40% less than those for Russet Burbank. Alturas requires 15% to 20% more water during the growing season than does Russet Bur-bank.     

Amey: A multipurpose, russet-skinned potato cultivar for the Eastern United States

Amey is a late-maturing, russet-skinned, whitefleshed potato cultivar that yields more than Russet Burbank in most eastern United States potato production areas. Tubers of Amey are mostly oblong, occasionally long, with an evenly russetted skin. Tubers of Amey are smoother, more attractive, and have a much lower incidence of external defects than Russet Burbank. The specific gravity of Amey is equal to or greater than the specific gravity of Russet Burbank. French fries produced from Amey are lighter than or equal in color to those produced from Russet Burbank; however, tubers are frequently not long enough to satisfy the french fry industry. Baking and taste quality of Amey are excellent, and it has potential as a fresh market potato. Amey is resistant to race Rol of the golden nematode, powdery scab, and common scab. It is moderately susceptible to Verticillium wilt. It is susceptible to potato leafroll virus, late blight, and early blight.     

Parenchyma cell growth in potato tubers I. Different tuber regions

Enlargement rates of starch-storage parenchyma cells during growth of Kennebec and Russet Burbank potato cultivars were determined for cortical, perimedullary, and pith tissuues of bud ends, midsections, and stem ends of tubers. Average volumetric size of parenchyma cells increased 7 to 18x during growth of Russet Burbank tubers, with the greatest increases occurring in cortical and perimedullary cells of bud ends and midsections, and the least in stem ends and pith tissues. In Kennebec tubers parenchyma cells in both stem end and midsection increased only 5 to 8 x, whereas increases in bud ends ranged from 8 to 20 times. Cell enlargement to tuber enlargement ratios appproached unity early in growth of Russet Burbank tubers. As tubers increased beyond the 45 g size, cell enlargement and tuber enlargement rates were essentially equal. Calculations of cells per unit tissue volume agreed with ratio determinations. The timing of such unity appeared to be delayed in Kennebec tubers, and was not quite as pronounced as in Russet Burbank tubers. This may have been due to differences in growth rates of individual tubers in response to cultural conditions. In general, cells of harvestmature Kennebec tubers were about 60% as large as similar cells of Russet Burbank tubers.     

Defender: A high-yielding, processing potato cultivar with foliar and tuber resistance to late blight

The potato cultivar Defender is high-yielding, white-skinned, and notable for having foliar and tuber resistance to late blight infection caused byPhytophthora infestans (Mont.) de Bary. It was released in 2004 by the USDA-ARS and the agricultural experiment stations of Idaho, Oregon, and Washington. Defender is suitable for processing into french fries and other frozen potato products directly from the field or from storage. Defender also may be used for fresh markets in regions such as California, where cultivars with long tubers and white skin are traditionally grown. Resistances to late blight and other potato diseases make Defender an ideal candidate for organic potato production. Defender consistently produced greater total and U.S. No. 1 yields than ‘Russet Burbank’ in Idaho trials. In early harvest trials conducted in the western U.S., average total yields of Defender were 17% and 23% greater than yields for ‘Ranger Russet’ and ‘Shepody’, respectively. In full-season trials conducted in the western U.S., Defender averaged 10% and 15% higher yields than Ranger Russet and Russet Burbank, respectively. Specific gravity of Defender is consistently high, with values comparable to those of Ranger Russet; tuber ascorbic acid (Vitamin C) levels are also high. In addition to late blight, Defender also is resistant to tuber early blight (Alternaria solani, (Ellis & G. Martin), L.R. Jones & Grout), potato virus X, and net necrosis; it has moderate levels of resistance to Verticillium wilt (Verticillium dahliae, Kleb), pink rot, foliar early blight, corky ringspot, and Erwinia soft rot. Defender is susceptible to scab (common and powdery) and potato leafroll virus; it has moderate susceptibility to dry rot (Fusarium spp.) and potato virus Y. Susceptibilities to internal necrosis, tuber greening, and blackspot bruise also have been noted, but may be minimized through cultural and harvest practices.     

Effect of short- and long-term exposure of seed tubers to low temperatures on the growth and yield of five potato cultivars

Trials were conducted in Alberta with Norchip, Norland, and Russet Burbank and in Ontario with Kennebec, Russet Burbank, Norchip, and Superior tubers to determine their response to short-term exposure to air temperatures of 0, ?1, and ?5 C and to long-term exposure to ?1, 0, 1,2, and 3 C. Exposure of seed tubers to ?1 C from 6 hours to 5 days did not affect growth characteristics or tuber yield of any of the five cultivars studied. Long-term (October–May) exposure to ?1 C in one study severely reduced emergence and tuber yield of Norchip (P<0.05). While the marketable yield of Russet Burbank was also reduced (P<0.05) by this treatment, Norland was not affected. Tubers of all cultivars exposed to air temperatures of ?3 or ?5 C for longer than 24 hours were severely injured and were not planted in the field trials at either location. In Alberta exposure of seed tubers of Norchip, Norland, and Russet Burbank to ?5 C for 6 and 12 hours caused a reduction (not significant) in yield. In Ontario, long-term storage at 1, 2, and 3 C and in combination with short-term (2 weeks) exposure to 0 or 10 C had no effect on growth or marketable yield of Norchip, Russet Burbank, Superior, and Kennebec seed tubers.     

Bannock russet: A dual-purpose, russet potato cultivar with high U. S. No. 1 yield and multiple disease resistances

Bannock Russet, a late-maturing, long russet potato cultivar, was released in 1999 by the USDA-ARS and the experiment stations of Idaho, Oregon, and Washington. It is suitable for processing into french fries and other frozen products. Its attractive russeted skin and excellent culinary qualities also make it suitable for fresh market. Bannock Russet, in comparisons with Russet Burbank, has consistently produced greater U.S. No. 1 yields in trials conducted throughout southern Idaho. In other western trial sites, total yields of Bannock Russet have been comparable to or smaller than that of Russet Burbank, but its percentage of undersize and cull potatoes is consistently less. Bannock Russet is resistant to potato virus Y (PVY), Verticillium wilt, common scab, and leafroll net necrosis. It also has moderate resistance to early blight (foliar and tuber) andErwinia soft rot. With respect to other potato diseases/disorders, Bannock Russet’s level of susceptibility is similar to that of Russet Burbank; the exception being shatter bruise, with Bannock Russet displaying a greater susceptibility. Cultural management guidelines have been developed to minimize shatter bruise in Bannock Russet. Bannock Russet’s multiple disease resistances coupled with its low nitrogen requirement, make it a low-input cultivar that could significantly reduce growers’ production costs.     

Modification of potato microtuber dormancy during induction and growthin vitro orex vitro

Prolonged or highly variable dormancy can be a significant impediment to the efficient use of potato (Solanum tuberosum L.) microtubers by the seed industry. In the present study, reductions in microtuber dormancy duration were obtained in cultivars commonly used by the processing industry (Kennebec, Russet Burbank and Shepody). This was achieved by modifying microtuber induction media and applying various dormancy-release treatments after harvest, with or without prior storage. An 8 h photoperiod, instead of continuous darkness during microtuber induction and development, increased microtuber yield while reducing dormancy duration. Dormancy duration was also shortened by increased sucrose concentration during microtuber induction under an 8 h photoperiod. As sucrose was increased from 4 to 16% under an 8 h photoperiod, mean dormancy duration decreased by 86 d for Shepody, 65 d for Kennebec and 46 d for Russet Burbank. During theex vitro storage period, 24 h treatment with bromoethane vapor (from 0.22 ml liquid BE per L volume) or bromoethane vapor followed by a 3 d treatment of 60% CO₂/ 20% O₂/ 20% N₂ resulted in a rapid dormancy release of freshly harvested microtubers. These dormancy-releasing treatments significantly increased minituber yields under greenhouse conditions for all cultivars when compared to untreated controls. Increased minituber yields were also observed when dormancy release treatments were applied to microtubers after storage at 6 C for 8 weeks. The results demonstrate that microtuber dormancy duration can be manipulated during growthin vitro orex vitro. However, optimization may require cultivarspecific protocols     

Potato Cultivar and Seed Type Affect the Development of Systemic <Emphasis Type="Italic">Potato virus Y</Emphasis> (PVY<Superscript>N-Wi</Superscript>) Infection

Potato virus Y (PVY) infection is one of the greatest challenges to seed potato production in the United States. To determine how cultivar and seed type affect the development of systemic PVY infection, Russet Burbank and Russet Norkotah Colorado 3 cultivars were grown from two types of pre-nuclear seed (i.e., plantlets and minitubers) and Generation 3 (G3) tubers and challenged with PVY strain Wilga (PVY^N-Wi). Systemic PVY infection was measured by assaying spread of virus from the inoculation site to upper non-inoculated leaves. The Burbank cultivar had a lower incidence of systemic PVY infection compared to the incidence of systemic PVY that developed in the Colorado 3 cultivar. Furthermore, Burbank plants grown from G3 tubers had a lower incidence of systemic PVY infection, as compared to Burbank plants grown from plantlets. Together our results indicate that both cultivar and seed type affect the development of systemic PVY^N-Wi infections post-inoculation.