Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines

Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid‐free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.     

Development of yellow-seeded Brassica napus of double low quality

Two yellow‐seeded white‐petalled Brassica napus F₇ inbred lines, developed from interspecific crosses, containing 26–28% emcic acid and more than 40 μmol glucosinolates (GLS)/g seed were crossed with two black/dark brown seeded B. napus varieties of double low quality and 287 doubled haploid (DH) lines were produced. The segregation in the DH lines indicated that three to four gene loci are involved in the determination of seed colour, and yellow seeds are formed when all alleles in all loci are in the homozygous recessive state. A dominant gene governed white petal colour and is linked with an erucic acid allele that, in the homozygous condition, produces 26–28% erucic acid. Four gene loci are involved in the control of total GLS content where low GLS was due to the presence of recessive alleles in the homozygous condition in all loci. From the DH breeding population a yellow‐seeded, yellow‐petalled, zero erucic acid line was obtained. This line was further crossed with conventional B. napus varieties of double low quality and, following pedigree selection, a yellow seeded B. napus of double low quality was obtained. The yellow seeds had higher oil plus protein content and lower fibre content than black seeds. A reduction of the concentration of chromogenic substances was found in the transparent seed coat of the yellow‐seeded B. napus.     

Quantitative genetic analysis of condensed tannins in oilseed rape meal

Condensed tannins (proanthocyanidins, PAs) in the seed meal of oilseed rape can potentially have a negative impact on non-ruminant livestock nutrition, particularly because of their ability to form indigestible, astringent or bitter-tasting complexes with proteins. One option to overcome this problem is the breeding of oilseed rape varieties with reduced condensed tannins in the seed coat. This might be achievable via selection of genotypes with thinner seed coats and consequently reduced condensed tannin accumulation (seed coat structural cell mutants), or alternatively by selection of genotypes with reduced biosynthesis of condensed tannins (flavonoid biosynthesis mutants). Both types of transparent testa (TT) mutants are well-characterised in Arabidopsis; however the genetic basis of the yellow-seed trait in the polyploid genome of rapeseed is still not completely understood. In this study, genetic and chemical analyses of PAs were performed in 166 doubled haploid (DH) rapeseed lines from the segregating Brassica napus doubled haploid population YE2-DH (black seed × yellow seed). Using these analyses, the relationship between seed colour and PA fractions in B. napus was investigated with a view to improving the rapeseed meal quality. Proanthocyanidin contents were estimated by vanillin and HPLC assays and the obtained values were used to identify quantitative trait loci. Closely linked molecular markers that were identified during this study for the target traits (seed colour, condensed tannins) can be valuable tools for breeding of new oilseed rape cultivars with reduced levels of antinutritive PA compounds.     

Evaluation of cytoplasmic effects on agronomic and seed quality traits in two doubled haploid populations of Brassica napus L.

Cytoplasmic effects have been occasionally implicated in the inheritance of several traits in oilseed rape (Brassica napus L.), including linolenic acid concentration (18:3) in the oil. It is important that these be considered when choosing the direction of cross for producing new breeding populations. To study this phenomenon, a reciprocal cross was made between two genotypes of oilseed rape, Reston and LL09, which differed for their erucic and linolenic acid concentrations in the seed oil. Two DH populations, which were produced by microspore culture from reciprocal F₁ plants, were evaluated in the growth room for one generation and in the field at two locations in Southern Ontario in 1993and 1994. Field notes were taken on days to flower, days to maturity,plant lodging, plant height and, seed quality traits. In the growth room study, the phenotypic distribution of 18:3 differed significantly between the two reciprocal DH populations. In the field, significant reciprocal differences between the population means were detected for 18:3,flowering date and protein content in both years and for days to maturity and oil content in 1993 only. To further study the parental lines,chloroplast (cp) and mitochondrial (mt) DNA from parental lines were isolated and subjected to RFLP and RAPD analysis. Several random primers revealed reproducible DNA polymorphism (RAPD) between the parental mt DNA. It is concluded that the direction of cross should be taken into consideration by oilseed rape breeders relying solely on doubled haploids for developing genotypes with modified seed quality traits in Brassica napus L. This revised version was published online in July 2006 with corrections to the Cover Date.     

The Effect of A Genome Substitution on the Resistance of Brassica napus to Infection by Leptosphaeria maculans

Six accessions belonging to four subspecies of Brassica rapa, including three accessions of B. rapa subsp. sylvestris, were crossed with B. oleracea subsp. alboglabra in order to develop a series of synthetic B. napus lines with a common C genome but contrasting A genomes. Different A genomes had significant effects on the efficiency of B. napus resynthesis and the sexual compatibility of the synthetic lines with oilseed rape cultivars. The synthetic lines were used to investigate the effect of A genome substitution on the resistance of B. napus to infection by Leptosphaeria maculans, and to explore the potential for the use of wild forms of B. rapa in oilseed rape breeding programmes. Synthetic lines derived from two wild accessions of B. rapa, and their F₁ hybrids with oilseed rape cultivars, expressed high levels of resistance to L. maculans in glasshouse experiments. One of these lines also expressed high levels of resistance in field experiments in England and Australia when exposed to a genetically diverse pathogen population. All other synthetic lines and cultivars were highly susceptible in both glasshouse and field experiments. F₁ hybrids between oilseed rape cultivars and synthetic lines derived from B. rapa subsp. chinensis were significantly more susceptible than either parent.     

Inheritance and potentials of a mutated dwarfing gene ndf1 in Brassica napus

A dwarf mutant ‘NDF‐1′, approximately 70 cm high, was derived from a 200‐cm high doubled haploid (DH) line ‘3529’ (Brassica napus), seeds of which were jointly treated with chemical inducers and bombardment of fast neutron. The leaves of the ‘NDF‐1’ mutant were wrinkled and thicker compared with the wild‐type control. The mutant had much lower values than its original parents for all agronomic traits, except for its seed weight. A genetic analysis revealed that dwarfism is under the control of a major gene (designated as ndf1) with a mainly additive effect and non‐significant dominance effect. Because of the high level of resistance to lodging, breeding programmes for double low dwarf oilseed rape and heterosis utilization were initiated. Some new dwarf strains with improved agronomic performance were developed. The hybrid of the cross between the tall parent and the dwarf line showed increased harvest index and significantly higher seed yield than the tall parent or the control variety ‘Zhongyou 821’ and presented an estimated heterosis vigour rate as high as 12.5–25.8%. The dwarf trait will be a promising marker for a simple, economic and efficient way to control the purity of F₁ hybrid varieties in hybrid production of B. napus.     

Quantitative trait locus analysis of seed germination,seedling vigour and seedling‐regulated hormones in Brassica napus

Good germination and seedling vigour are major breeding targets in winter oilseed rape (Brassica napus), because seedling vigour and prewinter crop establishment are closely associated with postwinter growth and yield. Here, we identified quantitative trait loci (QTL) related to germination, seedling vigour and seedling‐regulated hormones in a doubled haploid (DH) mapping population from a cross between winter oilseed rape parents with high vigour (Express 617) and low vigour (1012‐98). By phenotyping in a climate‐controlled glasshouse, we identified a total of 13 QTL on nine chromosomes for germination and seedling‐related traits at 7 and 14 days after sowing (DAS), explaining up to 11.2% of the phenotypic variation for seedling vigour. Forty‐seven metabolic QTL on 15 chromosomes were identified for auxin, abscisic acid (ABA) and dihydrophaseic acid (DPA) at 5 and 12 DAS, explaining up to 49.4% of phenotypic variation in seedling hormone composition. Multitrait QTL hot spots contribute to our understanding of the genetics and metabolomics of germination and seeding vigour in B. napus, and represent potential targets to breed high‐vigour cultivars.     

Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus)

The quality of the oil derived from oilseed rape is determined by its fatty acid composition. Breeding oilseed rape for enhanced oil quality includes the development of cultivars with high oleic and low linolenic acid. Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) techniques were investigated for the development of molecular markers for genes controlling oleic and/or linolenic acid. Markers that were identified were converted to sequence characterized amplified region (SCAR) markers for use in breeding. Molecular markers associated with these two fatty acids were identified in a doubled haploid population derived from a cross between the oilseed rape lines TO99‐5318‐20, very high oleic (>79%) and very low linolenic acid (<2%) × DH12075, high oleic (68%) and higher linolenic acid (>7%). Eight RAPD markers were associated with oleic and linolenic acid contents. The RAPD marker UBC 2₈₃₀ accounted for 43% and 13% of the genetic variation for oleic and linolenic acid levels, respectively. The RAPD marker UBC 153₅₅₀ accounted for 19% of the genetic variation for linolenic acid. The UBC 2₈₃₀ fragment was converted to a SCAR marker. The markers identified in this study should be useful tools for the early generation selection of high oleic and low linolenic acid genotypes in oilseed rape breeding programmes.     

A comparison of traditional and haploid-derived breeding populations of oilseed rape (Brassica napus L.) for fatty acid composition of the seed oil

Summary Microspore embryogenesis technology allows plant breeders to efficiently generate homozygous micros-pore-derived breeding populations of oilseed rape (Brassica napus L.) without traditional generations of inbreeding. This study was conducted to compare the frequency distribution of microspore-derived population and single seed descent populations with respect to fatty acids of seed oil. Both microspore-derived populations and single seed descent populations were produced from each of three crosses made between selected parents containing contrasting amount of erucic, oleic, linoleic and linolenic acids. The fatty acid content of F₃ plants derived lines (F₅ seed) developed by single seed descent was compared to that of microspore-derived populations. The means, ranges and distribution pattern of seed fatty acid contents were similar in both populations for each fatty acid studied, although a few heterozygous lines were observed in the single seed descent populations. The results indicated that microspore-derived population form random, homozygous F₁ plant derived gametic arrays for all fatty acids evaluated. Selection for altered fatty acid composition in microspore-derived and single seed descent homozygous populations should be equally efficient, in the absence of linkage of traits investigated.     

Genetic variation for sinapate ester content in winter rapeseed (Brassica napus L.) and development of NIRS calibration equations

Increasing the meal and protein quality of winter rapeseed (Brassica napus L.) for food and feed purposes is gaining importance in rapeseed breeding programmes. Rapeseed meal has a high content of phenolic acid esters, mainly sinapate esters, which have been shown to cause a dark colour and a bitter taste in rapeseed meal and derived protein products. The aim of the present study was to analyse the genetic variation for individual and total sinapate ester content, to develop Near Infrared Reflectance Spectroscopic (NIRS) calibrations, and to identify genotypes with a low sinapate ester content after testing in the field. The following sinapate esters were analysed by HPLC: sinapoylcholine (sinapine), sinapoylglucose, and a minor group of ‘other sinapate esters’ which includes free sinapate. A genotypically diverse set of seed samples of winter oilseed rape (old and new cultivars, breeding lines, resynthesized rapeseed) from different years and locations was collected, their NIRS spectra recorded and the samples were further analysed by HPLC. The complete NIRS calibration seed sample set (n = 575) showed a large variation in total sinapate ester content, ranging from 3.2 to 12.7 mg sinapate equivalents per g seeds. The NIRS calibration equations showed high fractions of explained variances in cross validation () ranging from 0.75 (other sinapate esters) to 0.85 (sinapoylglucose). The standard errors of cross validation (SECV) ranged from 0.38 (other sinapate esters) to 0.70 mg/g seed (total sinapate esters). In validation and in independent validations the predicted results were not always acceptable, indicating that the NIRS calibrations need to be extended by analysing samples from new populations. Following replicated field experiments, a doubled haploid line obtained from the old Dutch cultivar Mansholts’ Hamburger Raps, and related DH lines from the cross DH Mansholts’ × Express were confirmed to have a 30–40% lower sinapate ester content compared to check cultivars.     

Genetic relationships among resynthesized,semi-resynthesized and natural <Emphasis Type="Italic">Brassica napus</Emphasis> L. genotypes

The allopolyploidization event that created cultivated oilseed rape Brassica napus L, followed by intense breeding, reduced its genetic diversity. Resynthesized (RS) B. napus L. obtained by interspecific hybridization between genotypes of B. rapa L. and B. oleracea L. can be a valuable source for broadening genetic diversity in cultivated oilseed rape. In this study, we determined the extent of DNA polymorphism among natural accessions of oilseed rape, resynthesized B. napus, their parental species and double-low quality semi-RS lines carrying the Rfo gene. Using 10 selected primer combinations, 522 polymorphic AFLP markers were scored in the complete set of 100 Brassica sp. To detect relationships between these genotypes, a cluster analysis was performed using the Jaccard’s distance. Resynthesized allopolyploids clustered directly between their diploid parents. Cultivated accessions of oilseed rape created a compact group away from resynthesized allopolyploids as well as semi-RS lines. The natural oilseed rape group, which consists of 49 cultivars and breeding lines of oilseed rape, is characterized by lower genetic diversity than the group of 33 accessions of resynthesized oilseed rape, and the analysis showed that the double-low quality semi-RS lines represent a specific genetic variation of B. napus. The de novo resynthesized B. napus lines and the semi-RS lines of double-low quality generated from them, provide a significant opportunity for enrichment the gene pool of oilseed rape.     

Effect of genetic background on the target fatty acids of acyl-ACP thioesterase transgenes in Brassica napus

Acyl‐acyl carrier protein (ACP) thioesterase (TE) is involved in the biosynthetic fatty acid pathway of plants. Conventional canola lines transformed individually with the bay‐TE (Uc FatB1), elm‐TE (Ua FatB1), nutmeg‐TE (Mf FatB1) or Cuphea‐TE transgene (Ch FatB1), produce seed oil with modified fatty acid compositions. This study assessed the effects of genetic background, cytoplasm, maternal parent, and interaction of different TE transgenes, on the target fatty acids using F1 seeds and double haploid (DH) lines. The F₁ seeds were produced by crossing four TE transgenic parental lines and three non‐transgenic cultivars with distinct fatty acid compositions. The DH lines were developed from microspores of F₁ plants. DH lines from different crosses showed that genetic background does not have an effect on the relative levels of the target fatty acids of the same TE, indicating the stability of the substrate specificity of the TE within canola. However, significant effects of genetic background on the content of the major target fatty acids, lauric acid (C12:0) or palmitic acid (C16:0) depending on the TE, were observed. Expression of the TE in low erucic acid (C22:1) genotypes resulted in higher target fatty acid levels than expression in high C22:1 genotypes. Reciprocal crosses showed maternal effects, but not cytoplasmic effects. In addition, co‐expression of two different TE transgenes in the same seeds was observed. These results indicate the importance of selection for appropriate genetic backgrounds in order to maximize the expression of the target fatty acids of TE transgenes, and also indicate potential uses of TE co‐expression in modifying canola seed oil.     

Identification of QTLs for phenolic compounds in oilseed rape (<Emphasis Type="Italic">Brassica napus L.</Emphasis>) by association mapping using SSR markers

Association mapping identifies quantitative trait loci (QTLs) by examining the marker-trait associations that can be attributed to the strength of linkage disequilibrium between markers and functional polymorphisms across a set of diverse germplasm. In this study, association mapping was performed to detect QTL-linked and genome wide SSR markers linked to phenolic compounds of extraction meal in a population of 49 genetically diverse oilseed rape cultivars of dark-seeded, winter-type oilseed rape accessions. Correction for population structure was performed using 559 genome wide SSR markers. Results showed that seed colour is an important contributor to seed meal quality. Totally, 52 SSR markers linked to phenolic compounds were detected, five of them being QTL linked markers. Some of these markers were already mapped on Brassica napus chromosomes that contain known QTL controlling oilseed rape meal quality traits. Our results demonstrate that association mapping is a useful approach to complement and enhance previous QTL information for marker-assisted selection.     

New mutants of winter rapeseed (Brassica napus L.) with changed fatty acid composition

Seeds of the winter oilseed rape (Brassica napus L.) line PN 3756/93 were treated with ethyl methanesulphonate to induce mutations in the fatty acid biosynthetic pathway. The seed mutagenic treatment was repeated in the M₂ generation. After treatments, individual seed and plant selections were made for changes in fatty acid composition during several generations of inbreeding. Self‐pollinated plants with changed fatty acid compositions were inbred to obtain genetically homozygous and stable mutant lines. Two mutants, M‐10453 and M‐10464, with increased levels of oleic acid (approximately 76%) and reduced linoleic and linolenic acid contents (8.5% and 7.5%, respectively) were selected. Gene or genes controlling desaturation of oleic acid were probably mutated in these plants. The third mutant, M‐681 had a very low linolenic acid content (approximately 2.6%) and increased linoleic acid content (approximately 26%). This would suggest the occurrence of mutations in genes controlling linoleic acid desaturation. The results of selection work during several generations showed that the environment had substantial influence on the composition of seed oil. This made the search for mutants with modify fatty acid compositions difficult. The induced mutants are not directly usable as new varieties, but can be used as parents in crosses for the development of high quality rapeseed varieties.     

Components of a Cytoplasmic Male Sterility System in Resynthesized and Cultivated Forms of Oilseed Rape (Brassies napus L.)

Male sterile Brassica napus L. plants were found in breeding material which was used for the development of yellow-seeded oilseed rape. The genetic studies indicated that the male sterility was conditioned by the presence of maintamer genes in the nuclear backgrounds of two newly resynthesized B. napus lines, No7076 and No7406, in combination with a male sterility-inducing cytoplasm (S) which is frequently found in cultivated forms of B. napus. Test crosses with nap maintainer and restorer lines support the conclusion that the observed male sterility is of nap type. Furthermore, the Eco RI restriction pattern of mitochondrial DNA of the (S) cytoplasm was identical to that of the nap cytoplasm. Hence, we conclude that we have uncovered a new source of maintainer lines for the nap system which could potentially lead to the production of a better maintainer/restorer system for use in hybrid oilseed rape breeding programmes. However, more work is needed to reduce the glucosinolate content of the maintainer lines and to determine the factors controlling the phenotypic expression of the system.     

甘蓝型油菜DH群体主要品质性状相关性及主成分分析

为了研究甘蓝型油菜品质性状之间的相关性,以期有效的改良油菜的品质性状,将170份DH纯系群体于2009-2012年进行田间试验,检测油菜种子品质性状,对其表型、相关性及主成分进行分析。结果表明：亲本中芥酸含量的变异系数最大(相差15.53%)。油菜DH群体中硫甙含量的变异幅度最大(128.43μmol/g饼粕),饱和脂肪酸的变异幅度最小(1.97%)。DH群体的品质性状表型变异幅度显示出了超亲遗传的现象。除了芥酸,含油量与其他组分呈现出极显著的负相关,特别是与蛋白质含量的负相关系数最大(-0.72)；蛋白质含量与油酸、亚油酸、亚麻酸和硫甙含量均呈极显著的正相关,与含油量和芥酸呈极显著的负相关；除了蛋白质,其他组分含量皆与硫甙含量呈现出极显著或者不显著的负相关。通过主成分分析可将品质性状综合为油酸亚油酸酸因子,蛋白质因子,亚麻酸因子和硫甙因子,累积贡献率达到93.94%,基本反映甘蓝型油菜DH系群体脂肪酸组分信息。     

Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars*

Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and B. oleracea L. (CC, 2n = 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n = 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.     

Fatty acid composition of resynthesized Brassica napus and trigenomic Brassica void of genes for erucic acid in their A genomes

The fatty acid composition of seed oil of four interspecific hybrids, resulting from crosses between zero erucic acid Brassica rapa (AA), and high erucic acid Brassica alboglabra/Brassica oleracea (CC) and Brassica carinata (BBCC), void of erucic acid genes in their A‐genomes was examined. The erucic acid content in resynthesized Brassica napus (AACC) lines derived from these crosses was only about half that of the high erucic acid CC genome parents, indicating equal contributions of the two genomes to oil (fatty acid) synthesis and accumulation. The differences in C18 fatty acid synthesis between the parents were also evident in the resulting resynthesized B. napus plants. Hexaploid Brassica plants of the genomic constitution AABBCC, in which the AA genome was incapable of erucic acid synthesis, had lower erucic acid contents than the B. carinata (BBCC) parent. This is plausible considering the fact that the zero erucic acid AA genome contributes to oil synthesis in AABBCC plants, thus reducing erucic acid content.     

The Inheritance of Aliphatic Glucosinolates in Brassica napus

The inheritance of aliphatic glucosinolates was studied in crosses between synthetic B. napus lines and oilseed rape cultivars. Six unlinked loci are described which determine the aliphatic glucosinolate profile of B. napus. One locus regulates the presence or absence of propyl glucosinolates, while another regulates the expression of pentyl glucosinolates. Two loci regulate the removal of the terminal H₃CS-group from the amino acid derivative to produce alkenyl glucosinolates as opposed to methylthioalkyl and methylsulphinylalkyl glucosinolates, regardless of the length of the alkyl chain. Likewise, another two loci regulate the hydroxylation of both butenyl and pentenyl glucosinolates. The functional alleles at one of the hydroxylation loci results in significantly more hydroxylation than those at the other locus. The large number of aliphatic glucosinolates which have been described in Brassica thus results from an interaction between genes which regulate side chain elongation and genes which modify the structure of the side chain, regardless of its length. The implications of this study for the biosynthesis of aliphatic glucosinolates, the origin of B. napus and the potential to manipulate the leaf and seed glucosinolate profile of oilseed rape are discussed.     

Status and perspectives of breeding for enhanced yield and quality of oilseed crops for Europe

Vegetable oils are a high-value agricultural commodity for use in refined edible oil products and as renewable industrial or fuel oils, and as the world population increases demand for high-quality seed oils continues to grow. Worldwide the oilseed market is dominated by soybean (Glycine max), followed by oilseed rape/canola (Brassica napus). In Europe the major oilseed crop is oilseed rape (B. napus), followed some way behind by sunflower (Helianthus annuus) and other minor crops like linseed (Linum usitatissimum) or camelina (Camelina sativa). The seed oil of these crops is characterized by a specific quality, i.e. fatty acid composition and other fat-soluble compounds: Camelina and linseed oils are characterised by high contents of linolenic acid (C18:3); in sunflower very high-oleic (up to 90% C18:1) types exist in addition to classical high-linoleic (C18:2) oilseeds; in B. napus a broad diversity of oil-types is available in addition to the modern 00 (canola) type, e.g. high-erucic acid rapeseed or high-oleic and low-linolenic cultivars. Moreover, vegetable oils contain valuable minor compounds such as tocopherols (vitamin E). Increases of such contents by breeding have lead to value-added edible oils. After oil extraction, oilseed meals—such as rapeseed extraction meal—contain a high-quality protein that can be used as a valuable animal feed. However, in comparison to soybean the meal from oilseed rape also contains relatively high amounts of anti-nutritive fibre compounds, phenolic acids, phytate and glucosinolates. Breeding efforts with respect to meal quality are therefore aimed at reduction of anti-nutritive components, while increasing the oil content, quality and yield also remains a major aim in oilseed rape breeding. This review article provides a general overview of the status of oilseed production in Europe and uses examples from winter oilseed rape to illustrate key breeding aims for sustainable and high-yielding production of high-quality vegetable oil. Emphasis is placed on analytical tools for high-throughput selection of overall seed quality.