Pharmacologic effects in man of a specific serotonin-reuptake inhibitor

Fluoxetine (Li-ly 110140) caused a 63 percent inhibition of [3H]serotonin uptake into platelets obtained from normal volunteers to whom the drug was administered daily for 7 days. This dose had no effect on the usual pressor response produced by injections of norepinephrine or tyramine.     

Soluble HL-A7 antigen: localization in the beta-lipoprotein fraction of human serum

The HL-A7 antigen of human leukocytes occurs as a soluble, low-density lipoprotein in the serum of HL-A7-positive individuals. Its presence in high concentration may inhibit direct leukocyte grouping, leading to erroneous results. This finding affords an easy method for the preparation of monospecific cytotoxic antiserums by absorption with serum fractions rather than leukocytes.     

Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker

Administration of a monoclonal antibody (GK1.5) that recognizes the L3T4 marker present on helper T cells prevented the development of experimental allergic encephalomyelitis (EAE) in mice. Furthermore, treatment with GK1.5 reversed EAE when the antibody was given to paralyzed animals. In vivo injection of GK1.5 selectively reduced the number of L3T4+ cells in the spleen and the lymph nodes. These results suggest that manipulation of the human equivalent of the murine L3T4+ T-cell subset with monoclonal antibodies may provide effective therapy for certain autoimmune diseases.     

Genetic mapping of Ir locus in man: linkage to second locus of HL-A

Fifty-seven members of a family that spanned three generations were studied for antigen E and ragweed skin sensitivity and HL-A antigens. There was significant association between the haplotype HL-A 2-12 and antigen E skin hypersensitivity (F = .22 to .26) in this family. The map order is first locus of HL-A, second locus of HL-A, and IrE. These determinants are considered to be part of the linkage group HL-1.     

Genetics of the antibody response to dextran in mice

The immune response to dextran having the alpha-1,3 linkage may be under the control of antibody structural genes. Mice that respond well to this antigen produce antibody restricted with respect to light chain class (lambda) and to an antigenic determinant resulting from a particular heavy and light chain interaction. The response to dextran is controlled by a locus linked to the-heavy chain locus.     

嗜乳脂蛋白基因外显子7多态性及其与奶牛产乳性状的相关性研究

采用PCR-RFLP对奶牛嗜乳脂蛋白基因的第7外显子进行了扩增,并用HaeⅢ进行酶切,产生了2个等位基因A(331+170 bp)和B(295+201 bp).分析不同基因型对个体乳脂率、乳蛋白、乳糖、干物质和305 d产奶成年当量等生产性能的影响.结果表明:AA基因型和A等位基因的频率分布在所试的4个群体巾都较高(>60%),从基因型奶牛的乳脂率、乳蛋白含量显著高于BB基因型(P<0.05),同时乳固形物含量和乳糖的含量也高于BB基因型,但不存在显著性差异;另外,AA 基因型奶牛的305 d产奶成年当量则显著低于BB基因型(P<0.05).对305 d成年当量、乳脂率、乳蛋白质含量、乳干物质含量和乳糖含量A基因的加性效应分别为-M5.34 kg/,胎次、0.07%、0.09%、0.09%和0.07%,A等位基因的显性效应分别为187.72 kg/胎次、0.007%、0.08%、0.04%和0.04%.     

Suppression of the humoral antibody response in natural retrovirus infections

The feline leukemia virus (FeLV) frequently causes death by predisposing the host to acute infections by other pathogens rather than by inducing leukemia. In a previous study, cats infected with FeLV were found to have prolonged homograft rejection responses but there was no evidence that the humoral immune response was impaired. In the present study, the humoral response to the synthetic multichain polypeptide (L-tyrosine-L-glutamic acid)-poly-DL-alanine-poly-L-lysine, denoted (T.G)AL, was found to be significantly depressed in healthy cats that were naturally infected with FeLV compared to uninfected controls. In cats with persistent FeLV viremia the major antibody response to (T.G)AL, normally seen at days 9 to 14 after immunization, was both delayed and greatly reduced.     

Experimental allergic encephalomyelitis in the rat: response to encephalitogenic proteins and peptides

Lewis rats were used to determine the encephalitogenic activity of myelin basic protein of different species and of 45-residue fragments of basic protein. Basic protein from guinea pigs was more active than that from rats, and the fragments from the two species showed the same order of activity. Bovine basic protein was the least active of the intact proteins, and the respective fragment was inactive. Studies of serum-binding capacity did not support the hypothesis that blocking antibody played a role in this biological variation, whereas consideration of the amino acid sequences of the three fragments suggested that differences in primary structure, operating either at the sensitization or the effector phase of the immune response, could account for the variation.     

Homeostasis of the antibody response: immunoregulation by NK cells

When injected into mice, the synthetic double-stranded polynucleotide poly(inosinic) X poly(cytidylic) acid induces high natural killer (NK) cell activity within 4 to 12 hours. Induction of NK activity in mice immunized 2 or 3 days previously, or the addition of NK cells to cultures immunized in vitro 2 or 3 days previously, promotes early termination of the ongoing primary immunoglobulin M antibody response. A target for NK cells is a population of accessory cells that has interacted with antigen and is necessary for sustaining the antibody response. The inference is strong that NK cells induced normally by immunization also terminate the usual antibody response in vivo by elimination of antigen-exposed accessory cells.     

Development of a cELISA for effective detection of the antibody against H7 subtype of avian influenza virus

H7 avian influenza viruses(AIVs) normally circulated among birds before. From 1996 to 2012, human infections with H7 AIVs(H7 N2, H7 N3, and H7 N7) were reported in Canada, Italy, Mexico, the Netherlands, the United Kingdom and the USA. Until March 2013, human infections with H7 N9 AIVs were reported in China. Since then, H7 N9 AIVs have continued to circulate in both humans and birds. Therefore, the detection of antibodies against the H7 subtype of AIVs has become an important topic. In this stu...     

鸡抗猪大肠杆菌卵黄抗体的研制与应用

用猪致病性大肠杆菌 K88、K99、987P、F4 1 标准菌株制成的灭活油佐剂作为免疫原 ,待产蛋鸡免疫后收集高免卵黄 ,然后提取、纯化 ,精制成鸡抗猪大肠杆菌特异性高免卵黄抗体并用于临床 .结果表明该制剂安全性好 ,每头仔猪注射或口服 2-4m L剂量预防仔猪大肠杆菌性腹泻 ,效果明显     

甲壳类和贝类原肌球蛋白的广谱单克隆抗体制备与鉴定

原肌球蛋白（Tropomyosin, TM）被认为是甲壳类和贝类的主要过敏原,为了应对与日俱增的甲壳类和贝类过敏风险,本研究以南美白对虾（Litopenaeus vannamei）的TM富集液为抗原免疫BALB/c小鼠,通过杂交瘤细胞融合技术筛选获得1株南美白对虾过敏原TM的单克隆抗体细胞株2F8,并利用ELISA、Western Blot、生物信息学等方法对2F8单抗进行免疫学特性分析,确定其效价、特异性、抗体亚型、结合区域,以TM的特异性单抗2F8和兔多抗构建双抗夹心胶体金试纸条分析单抗2F8的应用可行性。结果显示,单抗2F8的抗体亚型为IgG1型、效价为1.28×105,抗体与TM抗原的IC50值为20.21,亲和力高;纯化的单抗2F8可以特异性识别甲壳类、贝类和鱿鱼的主要过敏原TM,存在3个潜在的TM抗原结合区域AA0-31,AA38-43和AA58-67。制备的试纸条检测结果显示,该试纸条可在10 min内目测5 ng/mL的南美白对虾TM（相当于12.5 μg/kg）,且可用于甲壳类和贝类肌肉中TM 的检测,研究表明所制备的2F8单抗亲和力高,可用于虾蟹贝和鱿鱼中的过敏原TM的鉴定与检测,具有较好的应用价值。本研究为过敏原TM的鉴定和检测提供了重要生物材料,也为今后TM的快速检测试剂盒开发提供了技术支持。     

Somatic cell genetic assignment of peptidase C and the Rh linkage group to chromosome A-1 in man

The segregation of the human peptidase-C phenotype in five different series of human-mouse hybrid clones was examined. The chromosome constitution of these hybrids was determined by quinacrine mustard fluorescence, Giemsa banding, and constitutive heterochromatin staining. That the clones could be classified without exception either as human peptidase C positive/ A-1 positive (14 clones), or as peptidase C negative/ A-1 negative (12 clones) indicates that peptidase C can be assigned to the human A-i chromosome. Data from an extensive series of human-mouse clones used provide support for the syntenic association between peptidase C and phosphoglucomutase-1 and by inference a linkage of both to Rh factor group.     

Genetic control of the antibody response: relationship between immune response and histocompatibility (H-2) type

The immune responses of inbred mice to a related series of three synthetic polypeptide antigens are genetically controlled traits which are closely correlated with the genotype for the major histocompatibility (H-2) locus. All strains of the same H-2 type exhibit the same pattern of immune response, independent of the remainder of a given strain's genetic background. There is marked antigen-specific polymorphism between strains of different H-2 types with respect to their patterns of response.     

小鼠B7-H4原核表达载体的构建、表达及多克隆抗体的制备

目的探讨小鼠B7-H4原核表达载体的构建、表达及多克隆抗体的制备。方法采用特异性引物扩增小鼠B7-H4（mB7-H4）胞外功能区DNA,经酶切、拼接构建原核表达载体pET28a-mB7-H4。将构建的重组表达质粒转化入E.coliBL21（DE3）菌株,采用IPTG诱导表达、Ni-NTA柱亲和层析纯化目的蛋白、SDS-PAGE分析蛋白纯度。将纯化的目的蛋白免疫家兔制备多克隆抗体,并对其进行纯化及鉴定。结果序列测定证实了构建的pET28a-mB7-H4重组表达载体含有mB7-H4编码序列,其序列分析与GenBank中公布序列对比一致,质粒在E.coli中诱导表达相对分子质量（Mr）为26.5 KD的目的蛋白,SDS-PAGE分析表明纯化后的目的蛋白达到电泳纯。双向琼脂扩散法检测抗体效价为1∶16,ELISA法检测抗体效价为1∶12 800,Western blot分析显示抗体能特异性结合mB7-H4。结论成功制备了高表达重组mB7-H4蛋白的原核表达载体及高效价抗mB7-H4多克隆抗体,为进一步研究B7-H4的功能奠定了基础。     

昆明鼠对猪轮状病毒核酸免疫的抗体应答

将昆明鼠随机分为A、B两组,分别肌肉注射含有猪轮状病毒(RV)JL94株VP7基因的重组真核表达质粒pcDNA-VP7和真核表达载体pcDNA3.1(+),每只鼠100μg·(100μL)-1,共免疫3次,每次间隔2周。首次免疫第0,14,21,28,35,39,47,54天采血分离血清用ELISA法检测抗体动态变化。A组小鼠血清在首免后第14天即可检出阳性抗体(P/N≥2.0)。     

HTLV-III in the semen and blood of a healthy homosexual man

Human T-lymphotropic virus type III (HTLV-III) is the probable etiologic agent for the acquired immune deficiency syndrome (AIDS). HTLV-III was isolated from semen and blood of a healthy homosexual man whose serum contains antibodies to HTLV-III. The finding of virus in semen supports epidemiologic data that suggest that AIDS can be transmitted sexually. In addition, the demonstration of HTLV-III in the blood and semen of a healthy individual establishes an asymptomatic, virus-positive carrier state which may be important in the dissemination of HTLV-III and, consequently, AIDS.     

PRRS弱毒株生殖道免疫对母猪阴道局部和全身IgA水平及特异性抗体水平的影响

猪繁殖与呼吸综合征(PRRS)弱毒株分别通过生殖道黏膜免疫和肌肉注射免疫母猪,再分别应用双夹心酶联免疫吸附试验(ELISA)技术检测阴道分泌物中分泌型免疫球蛋白A(SIgA)的水平和血液中免疫球蛋白A(IgA)水平,用竞争ELISA技术检测血液中PRRS特异性抗体的水平.结果表明:免疫后第3周,母猪生殖道免疫组阴道分泌物中SIgA水平显著高于对照组(P<0.05);血液IgA水平与对照组相比升高,但差异不显著;血液中PRRS特异性抗体水平与对照组相比也无显著差异.肌肉注射组母猪阴道分泌物中SIgA水平与对照组相比无显著差异.但血液中PRRS特异性抗体水平显著高于对照组(P<0.05).免疫后第5周生殖道免疫组阴道分泌物中SIgA水平和血液中IgA水平高于对照组,但无显著差异.肌肉注射组血液PRRS特异性抗体水平显著高于对照组(P<0.05).表明PRRS弱毒株经生殖道黏膜免疫能提高母猪生殖道局部SIgA的水平,但对血液中IgA和IgG水平影响不大.     

Crystal structure of a shark single-domain antibody V region in complex with lysozyme

Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.