Degradability of soils under oak and pine in Central Spain

The purpose of this research was to study the influence of the vegetation on the soil C pool of forests of pines (Pinus sylvestris) and oaks (Quercus pyrenaica), located in Central-Western Spain. Horizons from selected soils located in these forests were sampled, and the soil organic C (SOC) was determined. In addition, in vitro incubation experiments were carried out, under controlled conditions, to monitor the stability of SOC against the microbial activity. Soil humus fractions were isolated following a classical procedure of chemical fractionation using alkaline solutions, before and after the incubation experiment. A deeper O horizon was found under the pine forest than under oak one; however, higher SOC content was found in the oak site than that under pine one. During the in vitro mineralization process, a lower CO₂ production by the soil sample from pine forest was observed, in relation to that emitted by the oak soil. In addition, a lower humification degree was estimated for the soil humus under pines than for that under oaks. In conclusion, replacement of oaks by pines produced a decrease in SOC accumulation and a lower quality of humus in the forest soils.     

Natural abundance of <Superscript>15</Superscript>N in two cacao plantations with legume and non-legume shade trees

Natural abundance of ¹⁵N was sampled in young and mature leaves, branches, stem, and coarse roots of trees in a cacao (Theobroma cacao) plantation shaded by legume tree Inga edulis and scattered non-legumes, in a cacao plantation with mixed-species shade (legume Gliricidia sepium and several non-legumes), and in a tree hedgerow bordering the plantations in Guácimo, in the humid Caribbean lowlands of Costa Rica. The deviation of the sample ¹⁵N proportion from that of atmosphere (δ¹⁵N) was similar in non-legumes Cordia alliodora, Posoqueria latifolia, Rollinia pittieri, and T. cacao. Deep-rooted Hieronyma alchorneoides had lower δ¹⁵N than other non-N₂-fixers, which probably reflected uptake from a partially different soil N pool. Gliricidia sepium had low δ¹⁵N. Inga edulis had high δ¹⁵N in leaves and branches but low in stem and coarse roots. The percentage of N fixed from atmosphere out of total tree N (%N_f) in G. sepium varied 56–74%; N₂ fixation was more active in July (the rainiest season) than in March (the relatively dry season). The variation of δ¹⁵N between organs in I. edulis was probably associated to ¹⁵N fractionation in leaves. Stem and coarse root δ¹⁵N was assumed to reflect the actual ratio of N₂ fixation to soil N uptake; stem-based estimates of %N_f in I. edulis were 48–63%. Theobroma cacao below I. edulis had lower δ¹⁵N than T. cacao below mixed-species shade, which may indicate direct N transfer from I. edulis to T. cacao but results so far were inconclusive. Further research should address the ¹⁵N fractionation in the studied species for improving the accuracy of the N transfer estimates. The δ¹⁵N appeared to vary according to ecophysiological characteristics of the trees.     

Positive nitrogen balance of <Emphasis Type="Italic">Acacia mangium</Emphasis> woodlots as fallows in the Philippines based on <Superscript>15</Superscript>N natural abundance data of N<Subscript>2</Subscript> fixation

Nitrogen inputs from biological nitrogen fixation contribute to productivity and sustainability of agroforestry systems but they need to be able to offset export of N when trees are harvested. This study assessed magnitudes of biological nitrogen fixation (natural ¹⁵N abundance) and N balance of Acacia mangium woodlots grown in farmer’s fields, and determined if N₂ fixation capacity was affected by tree age. Tree biomass, standing litter, understory vegetation and soil samplings were conducted in 15 farmer’s fields growing A. mangium as a form of sequential agroforestry in Claveria, Misamis Oriental, Philippines. The trees corresponded to ages of 4, 6, 8, 10 and 12 years, and were replicated three times. Samples from different plant parts and soils (0–100 cm) were collected and analyzed for δ¹⁵N and nutrients. The B-value, needed as a reference of isotopic discrimination when fully reliant on atmospheric N, was generated by growing A. mangium in an N₂-free sand culture in the glasshouse. Isotopic discrimination occurring during N₂ fixation and metabolic processes indicated variation of δ¹⁵N values in the order of nodules > old leaves > young leaves > stems > litterfall and roots of the trees grown in the field, with values ranging from −0.8 to 3.5‰ except nodules which were enriched and significantly different from other plant parts (P < 0.0001). Isotopic discrimination was not affected by tree age (P > 0.05). Plants grown in N free sand culture exhibited the same pattern of isotopic discrimination as plants grown in the field. The estimated B-value for the whole plant of A. mangium was −0.86‰. Mature tree stands of 12 years accumulated up to 1994 kg N ha⁻¹ in aboveground biomass. Average proportion of N derived from N₂ fixation of A. mangium was 54% (±22) and was not affected by age (P > 0.05). Average yearly quantities of N₂ fixed were 128 kg N ha⁻¹ in above-ground biomass amounting to 1208 kg N fixed ha⁻¹ over 12 years. Harvest of 12-year old trees removed approximately 91% of standing aboveground biomass from the site as timber and fuel wood. The resulting net N balance was +151 kg N ha⁻¹ derived from remaining leaves, twigs, standing litter, and +562 kg N ha⁻¹ when tree roots were included in the calculation. The fast growing A. mangium appears to be a viable fallow option for managing N in these systems. However, other nutrients have to be replaced by using part of the timber and fuel wood sales to compensate for large amounts of nutrient removed in order for the system to be sustainable.     

Soil Carbon and Nitrogen Dynamics Followed by a Forest-to-pasture Conversion in Western Mexico

Gains and losses of soil carbon (C), have been reported when tropical forests are converted to pastures. Regional studies are crucial for setting regional baselines and explaining each particular trend, in order to solve this controversy. Tropical deciduous forest (TDF) is under high deforestation pressure, mainly for conversion to pastures. The present study compared soil organic C (SOC) and nitrogen (SON) in the surface layer (0–5 cm) of forest and pasture soils in a TDF of western Mexico. SOC and SON concentrations were 18 and 60% lower in pasture soils than in forest soils, and C:N ratio increased in pasture soils. Furthermore, pasture soils had lower labile C and available inorganic nitrogen (N) than forest soils. These results can be explained as a reduction in C inputs to pasture soils and management-induced disruption of soil aggregates. In forest soils, macroaggregates (> 250 μm) were predominant (85%), whereas in pasture soils they were reduced to 35% of dry sand-free soil mass. The estimated SOC and SON losses from the top 5 cm of soil were 3 Mg C ha⁻¹ and 0.9 Mg N ha⁻¹, respectively.     

High organic carbon stock in a karstic soil of the Middle-European Forest Province persists after centuries-long agroforestry management

Little is known on soil organic carbon (SOC) stocks in karst areas worldwide, although many of them have seen long-term application of agroforestry systems with a potential for carbon sequestration. Therefore, our study aimed to assess landscape-level SOC concentration and stock in the Silica Plateau, a part of the Slovak Karst Biosphere Reserve located in the Western Carpathians (Slovakia) with a centuries-long agroforestry record. The most represented local soil units are Chromi-Rendzic Leptosols and Chromic Cambisols with clayey loam texture, C/N ratio 9–12, and $ {\text{pH}}_{{{\text{H}}_{2} {\text{O}}}} $ 6.6–6.2 in their organo-mineral surface horizons. Mull surface humus form prevails under mixed forest stands dominated by hornbeam (Carpinus betulus L.), oak (Quercus petraea L.), and beech (Fagus sylvatica L.). A total of 2,700 soil samples were collected from 150 soil pits. Both SOC concentrations and stocks were determined for the 0–60?cm mineral soil layer. Soil stoniness was accounted for by means of electrical resistivity tomography. According to the analysis of covariance, cropland SOC concentration (0.026?g?g^?1) is significantly lower compared to forestland (0.040?g?g^?1) and pastureland (0.041?g?g^?1) (P?<?0.01). During the period of 130?years after forest clearing, cropland SOC stock has been reduced at an exponential decay rate of ca 0.002?year^?1, while the SOC stock in pastureland has increased following land use change from cropland by approximately 30% during the same period of time. Irrespective of land use history, overall SOC stock is high reaching on average 207.4?Mg?ha^?1, out of which 66% are stored within 0–30?cm and 34% within 30–60?cm soil layers.     

Production and quality of senesced and green litterfall in a pine–oak forest in central-northwest Mexico

Litterfall is an important ecological process in forest ecosystems, influencing the transfer of organic matter, carbon (C), nitrogen (N), phosphorous (P) and other nutrients from vegetation to the soil. We examined the production of different litterfall fractions as well as nutrient content and nutrient inputs by senesced and green leaf-litter in a semiarid forest from central Mexico. From September 2006 to August 2007, monthly litter sampling was carried out in monospecific and mixed stands of Quercus potosina and Pinus cembroides. Litterfall displayed a marked bimodal pattern with the largest annual amount (5993 ± 655 kg ha⁻¹ yr⁻¹) recorded in mixed stands, followed by Q. potosina (4869 ± 510 kg ha⁻¹ yr⁻¹), and P. cembroides (3023 ± 337 kg ha⁻¹ yr⁻¹). Leaves constituted the largest fraction of total litterfall reaching almost 60%, while small branches contributed with 20–30%. Overall, N content in leaf-litter was higher while lignin content was significantly lower for Q. potosina than for P. cembroides. Thus, greater litter quality together with higher litter production caused the largest C, N and P inputs to forest soils to occur in monospecific Q. potosina stands. Green leaf fall displayed significantly lower lignin:N and C:N ratios in Q. potosina than P. cembroides suggesting faster decomposition and nutrient return rates by the former. Although we recorded only two green leaf fall events, they accounted for 18% and 11% of the total N and P input, respectively, from leaf-litter during the study period. Apart, from the large spatiotemporal heterogeneity introduced by differences in litter quantity and quality of evergreen, deciduous and mixed stands, green litterfall appears to represent a much more important mechanism of nutrient input to semiarid forest ecosystems than previously considered.     

Does tree species composition control soil organic carbon pools in Mediterranean mountain forests?

We compared soil organic carbon (SOC) stocks and stability under two widely distributed tree species in the Mediterranean region: Scots pine (Pinus sylvestris L.) and Pyrenean oak (Quercus pyrenaica Willd.) at their ecotone. We hypothesised that soils under Scots pine store more SOC and that tree species composition controls the amount and biochemical composition of organic matter inputs, but does not influence physico-chemical stabilization of SOC. At three locations in Central Spain, we assessed SOC stocks in the forest floor and down to 50 cm in the mineral in pure and mixed stands of Pyrenean oak and Scots pine, as well as litterfall inputs over approximately 3 years at two sites. The relative SOC stability in the topsoil (0-10 cm) was determined through size-fractionation (53 μm) into mineral-associated and particulate organic matter and through KMnO₄-reactive C and soil C:N ratio.Scots pine soils stored 95-140 Mg ha⁻¹ of C (forest floor plus 50 cm mineral soil), roughly the double than Pyrenean oak soils (40-80 Mg ha⁻¹ of C), with stocks closely correlated to litterfall rates. Differences were most pronounced in the forest floor and uppermost 10 cm of the mineral soil, but remained evident in the deeper layers. Biochemical indicators of soil organic matter suggested that biochemical recalcitrance of soil organic matter was higher under pine than under oak, contributing as well to a greater SOC storage under pine. Differences in SOC stocks between tree species were mainly due to the particulate organic matter (not associated to mineral particles). Forest conversion from Pyrenean oak to Scots pine may contribute to enhance soil C sequestration, but only in form of mineral-unprotected soil organic matter.     

Effect of tree species on carbon stocks in forest floor and mineral soil and implications for soil carbon inventories

Forest soil organic carbon (SOC) and forest floor carbon (FFC) stocks are highly variable. The sampling effort required to assess SOC and FFC stocks is therefore large, resulting in limited sampling and poor estimates of the size, spatial distribution, and changes in SOC and FFC stocks in many countries. Forest SOC and FFC stocks are influenced by tree species. Therefore, quantification of the effect of tree species on carbon stocks combined with spatial information on tree species distribution could improve insight into the spatial distribution of forest carbon stocks.We present a study on the effect of tree species on FFC and SOC stock for a forest in the Netherlands and evaluate how this information could be used for inventory improvement. We assessed FFC and SOC stocks in stands of beech (Fagus sylvatica), Douglas fir (Pseudotsuga menziesii), Scots pine (Pinus sylvestris), oak (Quercus robur) and larch (Larix kaempferi).FFC and SOC stocks differed between a number of species. FFC stocks varied between 11.1 Mg C ha⁻¹ (beech) and 29.6 Mg C ha⁻¹ (larch). SOC stocks varied between 53.3 Mg C ha⁻¹ (beech) and 97.1 Mg C ha⁻¹ (larch). At managed locations, carbon stocks were lower than at unmanaged locations. The Dutch carbon inventory currently overestimates FFC stocks. Differences in carbon stocks between conifer and broadleaf forests were significant enough to consider them relevant for the Dutch system for carbon inventory.     

Partitioning of applied <Superscript>15</Superscript>N fertilizer in a longleaf pine and native woody ornamental intercropping system

The cultivation of ornamentals to produce woody floral products—the fresh or dried stems that are used for decorative purposes—may be an attractive option for southeastern landowners looking to generate income from small landholdings. Since many shrubs native to the understory of the longleaf pine (Pinus palustris Mill.) ecosystem have market potential, one possibility is the intercropping of select species in the between-row spacing of young longleaf pine plantations. The objective of this study was to evaluate how interspecific competition affects the fate of ¹⁵N fertilizer when American beautyberry (Callicarpa americana L.), wax myrtle (Morella cerifera (L.) Small) and inkberry (Ilex glabra (L.) A.Gray) are intercropped with longleaf pine. Nitrogen derived from fertilizer (NDF), utilization of fertilizer N (UFN) and recovery of fertilizer N (RFN_soil) were compared between agroforestry and monoculture (treeless) treatments to assess the effects of competition. Results varied by species, with NDF being higher for C. americana foliage and lower for all M. cerifera tissues in the agroforestry treatment. No effect was observed for I. glabra. UFN was lower for all species in the agroforestry treatment. RFN_soil was higher in the agroforestry treatment for I. glabra, but no treatment effects were observed for C. americana or M. cerifera. Overall, while it is clear that interspecific competition was present in the agroforestry treatment, the inefficiency of fertilizer use suggests that nitrogen was not the most limiting resource. Management interventions, particularly those that address competition for water, will likely be critical to the success of this system.     

Measuring nitrogen fixation by <Emphasis Type="Italic">Sesbania sesban</Emphasis> planted fallows using <Superscript>15</Superscript>N tracer technique in Kenya

A field experiment was performed in eastern Kenya to estimate N₂ fixation by Sesbania sesban over an 18-month period using the ¹⁵N dilution method. The influence of three reference species, Senna spectabilis, Eucalyptus saligna and Grevillea robusta, on the estimates of N₂ fixation was also assessed. Percentage Ndfa (nitrogen derived from the atmosphere) was calculated based on foliar atom excess (FAE), above-ground atom excess (AAE) or whole tree atom excess (WAE) data. The differences in atom% ¹⁵N excess values between species and plant parts are presented and discussed. We recommend the use of several reference species for estimating %Ndfa and that the different results obtained should be carefully considered in relation to the issues being addressed. In this study, Senna was the most suitable of the three reference species because its N uptake pattern and phenology were very similar to those of Sesbania. When well established, the amount of N fixed by Sesbania accounts for more than 80% of its total N content, according to FAE-based estimates. We estimated the Ndfa by Sesbania after 18 months to between 500 and 600 kg ha⁻¹ , depending on whether FAE, AAE or WAE data were used and on the choice of reference species. The substantial accumulation of N in planted Sesbania highlighted its potential to increase the sustainability of crop production on N-limited soils. We consider the ¹⁵N dilution method to be appropriate for quantifying N₂ fixation in improved fallows in studies, similar to this one, of young trees with high N₂-fixing ability.     

Characterization of sp<Superscript>2</Superscript>- and sp<Superscript>3</Superscript>-bonded carbon in wood charcoal

Japanese cedar (Cryptomeria japonica) preheated at 700°C was subsequently heated to 1800°C and characterized by electron microscopy, X-ray diffraction, and micro-Raman spectroscopy. The degree of disorder of carbon crystallites and the amount of amorphous phase decreased considerably with an increase in heat treatment temperature to 1400°C, while carbon crystallites clearly developed above this temperature, showing that the microstructure of carbonized wood undergoes drastic changes around 1400°C. Besides showing the bands for sp²-bonded carbon, the Raman spectra showed a shoulder near 1100 cm⁻¹ assigned to sp³-bonded carbon. With an increase of heat treatment temperature, the peak position of the Raman sp³ band shifted to a lower frequency from 1190 to 1120 cm⁻¹, which is due to the transformation of sp³-bonded carbon from an amorphous phase to a nanocrystalline phase. These data showed that the microstructure of carbonized wood from 700° to 1800°C consisted of the combination of sp²- and sp³-bonded carbon, which is probably due to the disordered microstructure of carbonized wood. It is suggested that the sp³-bonded carbon is transformed from an amorphous structure to a nanocrystalline structure with the growth of polyaromatic stacks at temperatures above 1400°C.     

Vertical distribution and soil organic matter composition in a montane cloud forest, Oaxaca, Mexico

In montane cloud forests (MCF), the main soil organic carbon (SOC) pool is believed to be constituted by organic debris accumulated on soil surface and, to a lesser extent, by the organic fraction associated with the mineral matrix. The vertical distribution of SOC within soil has strong implications on the composition, stabilization and turnover of the soil organic matter (SOM). In ecosystems like MCF, where the climatic and edaphic conditions varied with altitude, the SOM accumulation and stabilization mechanisms possibly respond to these changes. For that reason, we studied the vertical distribution, accumulation and chemical composition of SOM in five montane cloud forest communities located between 1,500 and 2,500?m a.s.l. Two main SOC accumulation patterns were found: one at 1,500, 1,950 and 2,400?m a.s.l., with SOC content gradually decreasing with depth (cumulative); and another at 2,050 and 2,500?m a.s.l. where SOC had a strong maximum in the surface horizon and a less pronounced increase the spodic horizon (eluviation–illuviation pattern). The total SOC pool in soil decreased in inverse relation to altitude from 227?C?ha^?1 at 1,500?m a.s.l. down to 143?mg?C?ha^?1 at 2,500?m a.s.l. About 40–60?% of total SOC content corresponded to the surficial organic horizon. The chemical fractionation of the SOM denoted in general predominance of the fulvic acid fraction, and high content of humin and humic acid fractions. We considered that the main SOC vertical distribution processes were related to the raw humus accumulation, decomposition in situ, podzolization in the eluviation–illuviation pattern soils mainly.     

Factors determining enzyme activities in soils under <Emphasis Type="Italic">Pinus halepensis</Emphasis> and <Emphasis Type="Italic">Pinus sylvestris</Emphasis> plantations in Spain: a basis for establishing sustainable forest management strategies

Key message

Water availability and soil pH seem to be major constraints for enzyme activities in calcareous soils under Pinus halepensis and acidic soils under Pinus sylvestris plantations respectively. Proposals for improving enzyme activities may include the promotion of broadleaf species to increase soil pH and the modulation of stand density or the implementation of soil preparation techniques to facilitate water infiltration.

Context

Soil enzymes play a key role in nutrient turnover in forest ecosystems, as they are responsible for the transformation of organic matter into available nutrients for plants. Enzyme activities are commonly influenced by temperature, humidity, nutrient availability, pH, and organic matter content.

Aims

To assess the differences between enzyme activities in calcareous soils below Pinus halepensis and acidic soils below Pinus sylvestris plantations in Spain and to trace those differences back to edapho-climatic parameters to answer the questions: Which environmental factors drive enzyme activities in these soils? How can forest management improve them?

Methods

The differences in climatic, soil physical, chemical, and biochemical parameters and the correlations between these parameters and enzyme activities in soils were assessed.

Results

Low pH and high level of phenols in acidic soils under Pinus sylvestris and water deficit in calcareous soils under Pinus halepensis plantations appeared to be the most limiting factors for enzyme activities.

Conclusion

Options such as the promotion of native broadleaf species in the Pinus sylvestris stands and the modulation of Pinus halepensis stand density or the implementation of soil preparation techniques may improve enzyme activities and, therefore, nutrient availability.

     

Organic matter composition and dynamics in a northern hardwood forest ecosystem 15 years after clear-cutting

Soil organic matter (SOM) plays an important role in governing soil properties and nutrient cycling in forest ecosystems. Clear-cutting alters the SOM cycle by changing decomposition rates and organic matter (OM) inputs to the forest ecosystem. We studied the 15-year clear-cutting response on the properties and composition of SOM at the Hubbard Brook Experimental Forest (HBEF) in New Hampshire. Solid-state ¹³C nuclear magnetic resonance (NMR) spectroscopy was used to study the structural chemistry of SOM in whole soils and extracted humic substances. Overall, alkyl C and O-alkyl C were the dominant C fractions in soils and humic substances. Alkyl C accounted for 38–49% of the total NMR signal intensity in soils and 33–56% in humic substances. O-alkyl C accounted for 32–45% of the signal intensity in soils and 20–31% in humic substances. Following clear-cutting, the contribution of O-alkyl C increased in whole soils and humic acids of the Oa horizon, while alkyl C decreased in whole soils and humic acids of Oa and Bh horizons. Thus, the ratio of alkyl C to O-alkyl C, an index of the degree of decomposition of SOM, decreased in whole soils and humic acids after clear-cutting, indicating that the SOM in post-harvest soils is less decomposed relative to pre-harvest soils. On average, humic substances accounted for 47% of SOM. The concentration of humic acid decreased by up to 25% in Oa, E and Bh horizons after clear-cutting, while the concentration of fulvic acid decreased by more than 40% in the Oa and E horizons. Together, these results indicate that clear-cutting resulted in the loss of humic substances from the forest floor and upper mineral horizons, which was replaced by less decomposed OM in the post-clear-cut soils under the regrowing forest.     

Interactions amongst trees and crops in <Emphasis Type="Italic">taungya</Emphasis> systems of western Kenya

Lack of empirical data on the effects of the taungya system on establishment and early growth of softwood plantations have partly contributed to controversial decisions regarding the continued suitability of the system for plantation establishment in Kenya. This study examined effectiveness of taungya systems of forest plantation establishment using Cupressus lusitanica and Pinus patula trees with Zea mays (maize) as a test intercrop on two contrasting site types (deep and shallow soils) in Mt. Elgon forest, western Kenya . Four treatments were evaluated in each site: trees with or without weed control, trees intercropped with maize, and sole maize. Results showed that tree survival, growth and nutrient uptake, and maize growth and yield were higher in the deep soil site than the shallow site. The t aungya system improved tree survival and growth, effects being greater in the deep than the shallow soil site. Both Cupressus lusitanica and Pinus patula trees had the same effects on maize growth and yield, reducing maize growth by 41–48% in the deep soil sites, and by 16–26% in the shallow site. Vector nutrient analysis and vector competition analysis of the treatment effects on growth and nutrient uptake of the trees and the maize crop suggested competition for N on the deep soils, but competition for K and P on the shallow soils. The study has demonstrated the applicability of graphical vector competition analysis in diagnosing tree–crop interactions in agroforestry.     

Farms, fires, and forestry: Disturbance legacies in the soils of the Northwest Wisconsin (USA) Sand Plain

We studied the long-term effects of disturbance within the Northwest Wisconsin (USA) Sand Plain (NWSP), an ecoregion that is characterized by very sandy soil and an active disturbance history that includes fire, agriculture and industrial forestry, largely clearcut logging of jack pine (Pinus banksiana) and aspen (Populus spp.). Open “barrens” communities on this landscape were formerly maintained by fire, and are a high conservation priority. Hill's Oak (Quercus ellipsoidalis) can also dominate forest canopies, while blueberry (Vaccinium angustifolium), and sweetfern (Comptonia peregrina) are common shrub species. We structured a field sampling design with a spatial-temporal database built from historic airphotos (1938 and 1997) and fire records to examine whether soil organic matter and nutrients vary with disturbance history in the nonforest habitats of the sand plain. We sampled soils along 83 transects, randomly stratified among five sampled classes: (1) nonforest-farming history; (2) nonforest-fire history; (3) nonforest-clearcut only history; (4) evergreen forest of jack pine and red pine (P. resinosa); and (5) deciduous forest of Hill's oak and aspen. Logging of the original forest took place in the late 1800s–early 1900s. The farms were abandoned between 1938 and 1960, and the most recent fire occurred in 1977. Thus, the duration of the agricultural legacy is approximately 45–65 years while observed fire effects have lasted for 26 years.We observed strong agricultural legacies, including high P and low OM, N and Ca. One possible explanation for the N legacy is that it is tied to soil OM accretion which may be driven by plant growth. We detected no difference in mean values for any of the soil properties between soils from nonforested areas within the Five-Mile fire and soils from nonforested areas with a clearcut-only history. We did observe a fire effect in high variance for soil P. This could have resulted from variations in fire severity and ash convection and deposition.Forest soils generally had lower pH than the nonforest soils, and the deciduous forest soils had the lowest pH and also very low Ca. We also observed high within-transect coefficient of variation for Ca in the forest soils.We conclude that agriculture is a qualitatively different disturbance-type than fire or clearcutting, that disturbance legacies tend to be most persistent with geologically stable elements, such as P, and that management and conservation planning within the NWSP would benefit from site-specific agricultural history, as well as attention to Ca.     

Properties of humic acids in Mediterranean forest soils (Southern Italy): influence of different plant covering

The chemical and spectroscopic properties of humic acids (HAs) isolated from four litters and their corresponding underlying soils at three depths in a protected forest area in Southern Italy were investigated as a function of four different plant coverings: Quercus ilex L., mixed Carpinus betulus L. and C. orientalis Mill., Pinus halepensis L., and mixed Quercus trojana Webb. and Q. ilex L.. The forested site is a part of a calcareous plateau, characterized by homogeneous soils classified as Eutric Cambisols associated with Calcic Luvisols. The changes in the composition of HAs with soil depth have been evaluated on the basis of chemical (elemental and COOH groups) and spectroscopic analyses (E₄/E₆ ratio and FT IR spectra), and lignin-derived CuO oxidation products. A different distribution of the main elements was found in the various HAs which is apparently related to the type of humic acid-precursor biomolecules in the parent litters. The HAs isolated from soils under Q. ilex and mixed Carpinus species showed a slight increase in the C/H ratio and COOH content downward the soil profile, suggesting increasing aromatic polycondensation and humification degree with depth. On the contrary, no trend was observed for HAs from soils under Pinus halepensis L. and mixed Quercus species, indicating a partial incorporation of residues deriving from litter degradation into these HAs. Further, the content of lignin-derived phenols was higher in Pinus halepensis L. and mixed Quercus species layers, with the same trend measured for the corresponding HAs, thus confirming a lignin contribution related to the lignin type of plant covering.     

Effects of continuous nitrogen addition on microbial properties and soil organic matter in a <Emphasis Type="Italic">Larix gmelinii</Emphasis> plantation in China

Continuous increases in anthropogenic nitrogen (N) deposition are likely to change soil microbial properties, and ultimately to affect soil carbon (C) storage. Temperate plantation forests play key roles in C sequestration, yet mechanisms underlying the influences of N deposition on soil organic matter accumulation are poorly understood. This study assessed the effect of N addition on soil microbial properties and soil organic matter distribution in a larch (Larix gmelinii) plantation. In a 9-year experiment in the plantation, N was applied at 100 kg N ha^?1 a^?1 to study the effects on soil C and N mineralization, microbial biomass, enzyme activity, and C and N in soil organic matter density fractions, and organic matter chemistry. The results showed that N addition had no influence on C and N contents in whole soil. However, soil C in different fractions responded to N addition differently. Soil C in light fractions did not change with N addition, while soil C in heavy fractions increased significantly. These results suggested that more soil C in heavy fractions was stabilized in the N-treated soils. However, microbial biomass C and N and phenol oxidase activity decreased in the N-treated soils and thus soil C increased in heavy fractions. Although N addition reduced microbial biomass and phenol oxidase activity, it had little effect on soil C mineralization, hydrolytic enzyme activities, δ¹³C value in soil and C–H stretch, carboxylates and amides, and C–O stretch in soil organic matter chemistry measured by Fourier transform infrared spectra. We conclude that N addition (1) altered microbial biomass and activity without affecting soil C in light fractions and (2) resulted in an increase in soil C in heavy fractions and that this increase was controlled by phenol oxidase activity and soil N availability.     

Allometric model of the Reineke equation for Japanese cypress (<Emphasis Type="Italic">Chamaecyparis obtusa</Emphasis>) and red pine (<Emphasis Type="Italic">Pinus densiflora</Emphasis>) stands

An allometric model that explains the mechanism of the difference in the slope of the Reineke equation (A) among species was proposed based on the allometric relationships of mean tree height (H) to quadratic mean diameter D (H ∝ D ^θ ) and stand density N (H ∝ N ^δ ), i.e., A = θ/δ. The proposed model was fitted to Japanese cypress (Chamaecyparis obtusa Endl.) and red pine (Pinus densiflora) stands. The allometric exponents θ and δ were, respectively, 0.8995 and −0.5000 for cypress and 0.8612 and −0.6619 for pine. The difference between cypress and pine was significant for δ but not for θ. Inserting the exponents into the model resulted in predicted slopes of −1.7991 for cypress and −1.3011 for pine. The difference in the slope of the Reineke equation between the two species was produced by characteristics related to the tree crown, rather than characteristics related to stem slenderness. The proposed model enables us to estimate the slope of the Reineke equation from commonly measured stand attributes, such as mean tree height and quadratic mean diameter. Therefore, the proposed model is expected to be practical and convenient for estimating the slope of the Reineke equation and for explaining the mechanism of its variation among species. The model should be also accepted as a generalized model of the stand density versus quadratic mean diameter relationship, whereas the original Reineke equation should be seen as a specific case of this model.     

Impact of plant species on the formation of carbon and nitrogen stock in soils under semi-desert conditions

The unique forest ecosystems investigated were created on the place of natural steppe biogeocoenoses 60?years ago. The aim of the study was to elucidate the effect of plant species on the formation of organic C and N stocks in soils and to estimate nitrogen availability for artificial wood plantation. For this purpose, 290 soil samples were taken from four forest monocultures (Quercus robur L., Pinus sylvestris L., Cotinus coggygria Scop., and Acer tataricum L.) and from virgin steppe ecosystem. The amounts and stocks of organic C, total and readily nitrified N, and seasonal dynamics of NO₃ ^? and NH₄ ⁺ ions activities were determined. It was shown that the species composition of the stands influenced the stock of organic C and N in soils. The storages of C and total N differed by 74 and 4.4?Mg/ha^?1, respectively, in the litter and upper horizons (0–40-cm layer) in the stands studied. The differences in distribution of stocks of these elements in virgin steppe and artificial forest ecosystems were found. Organic C and N stocks increased 1.6–6.6 times in the forest litter compared to the steppe one, while in 5–40-cm layer, the storages of C and N decreased by 20–35% compared to the virgin soil. The impact of litter on total N content in arid climate was limited in 0–5-cm layer. The deficit of mineral N compounds was observed in autumn in soil with low stock of total N.