The effect of temperature on growth and dry matter production of lychee seedlings

In lychee (Litchi chinensis Sonn.), high day/night temperatures ($\text{30}\text{25}$, $\text{25}\text{20}$, $\text{20}\text{15}\text{°}\text{C}$ compared to $\text{15}\text{10}\text{°}\text{C}$) increased stem extension, node and leaf production and plant dry weight in seedling selections of 6 cultivars (‘Bengal’, ‘Haak Yip’, ‘Kwai May’, ‘Gee Kee’, ‘Tai So’ and ‘Wai Chee’). The mean daily base temperature for shoot growth (node production) was estimated by linear correlation to be 11.0°C for the seedlings. High temperatures also increased the proportion of leaf and decreased the proportion of root in the total dry weight. There was a strong effect of cultivar on all aspects of growth, but the responses to temperature were similar.This experiment shows that temperatures in many tropical and warm sub-tropical areas where lychees are grown are too high to induce satisfactory vegetative dormancy prior to floral initiation. The assessment of lychee seedlings for low vigour in a heated glasshouse appears to be a useful technique for the initial screening of adapted genotypes (which flower and fruit under warm conditions) prior to field evaluation.     

Environmental and chemical control of growth and flowering of Chamelaucium uncinatum Schauer

The main factor affecting floral initiation of Geraldton Wax-Flower (Chamelaucium uncinatum) is the photoperiod, while temperature is the major factor affecting flower development. Four weeks of short days (SD) are generally required for obtaining full flowering. The number of flowers produced per plant increases with increasing the number of SD. Under mild temperatures of $\text{20}\text{14°}\text{C}$ (day/night), plants initiated flowers even in long days (LD). However, fewer flowers were produced and on higher nodes as compared to SD plants. Chlormequat promoted flowering under prevailing summer conditions of high temperatures and LD. Under prevailing autumn conditions favourable for flower initiation, LD treatment or weekly sprays with gibberellic acid (GA) reduced the number of flowers per plant. Combined treatment of LD and GA reduced both the flowering percentage and the number of flowers per plant. Discontinuing the LD or the GA treatments caused a resumption of full flower initiation.     

An analysis of the growth of forced tulips. 1. Changes in plant fresh and dry weights during growth at two temperatures

Changes in the fresh and dry weights of the component parts of plants of tulip cv. ‘Apeldoorn’ were followed in bulbs kept at low and high temperatures (9 and 18°C respectively) from the time of completion of flower differentiation until anthesis.There were marked differences between shoot dry weights at the two temperatures. At 9°C the stem, leaves and flower grew exponentially throughout the whole period, but at 18°C the specific growth rate of the stem and leaves declined throughout the period of the experiment. At 9°C the proportion of total dry weight in shoots and daughter bulbs was higher than at 18°C, and the proportion in the mother bulb was correspondingly smaller.At both temperatures the $\text{fresh weight}\text{dry weight}$ ratios of the mother bulb, shoot and daughter bulbs declined during dry storage, the decline being greater at 9°C. After planting, the ratio for all plant parts increased at 9°C, but scarcely changed at 18°C.These results are discussed in relation to dormancy, to the low-temperature requirement for successful and rapid flowering and to flower quality.     

Effects of air temperature,soil temperature and soil moisture on growth and development of tomato itself and grafted on its own and egg-plant rootstock

The problem was studied whether tomatoes, grown in a hot and arid climate, benefit from grafting on egg-plant, which is highly efficient in water uptake. Growth and development of tomato (T), tomato grafted on its own rootstock ($\text{T}\text{T}$) and tomato grafted on egg-plant rootstock ($\text{T}\text{E}$) were compared at air temperatures of 28°C during the day and 18°C during the night ($\text{28}\text{18}$) and at 28°C constantly ($\text{28}\text{28}$), at soil temperatures of 14, 21 and 28°C with the following soil moisture regimes: wet (W₁), medium (W₂) and dry (W₃).At $\text{28}\text{18}$ and $\text{28}\text{28}$ water consumption was about equal, but the transpiration ratio at $\text{28}\text{28}$ was twice as high as that at $\text{28}\text{18}$. The latter conditions gave a much stronger plant with more fruits. At a soil temperature of 14°C water use was strongly reduced. The transpiration ratio increased with the soil temperature. Differences in plant type were small. At the highest soil temperature of 28°C fruit growth was strongly reduced. At lower soil moisture levels less water was used and the transpiration was lower. Plant type was correlated herewith.Vegetative growth of $\text{T}\text{T}$ was weaker than of T, but generative growth was stimulated. The strong E rootstock stimulated vegetative growth at high air and soil temperature, but fruit growth was very poor under these conditions; at a low soil temperature of 14°C vegetative growth was also reduced.The hope that the E rootstock would be beneficial for fruit growth at high temperatures was not fulfilled.An additional experiment in a growth-room at 23°C showed that under conditions of moisture stress there was no difference in water potential between leaves of $\text{T}\text{T}$ and $\text{T}\text{E}$.     

Temperature influences nutrient absorption and uptake rates of bananas grown in controlled environments

We explored the influence of temperature on the concentration of nutrients in banana plants, the nutrient uptake rate, apparent root transfer coefficient ($\text{}\text{?}$ga) and the relationship between accumulation of dry matter and nutrient. Young banana plants (Musa (AAA group, Cavendish sub-group) ‘Williams’) were grown at six temperature regimes (17/10–37/30°C) in sunlit growth chambers for 12 weeks.The amount of nutrient absorbed was influenced by the amount of growth made, but the link between the two differed from one element to another. Temperatures less than $\text{29}\text{22°}\text{C}$ reduced the concentration of all elements in the whole plant, except Fe.Temperature influenced the root uptake rate of B 10-fold; K, Na, Ca, Fe and Zn 3–4-fold; and N, P, Mg, Mn, Cu, Cl 1–2-fold. Among the elements the highest recorded rate was 12 mg g^?1 day^?1 for K at $\text{33}\text{26°}\text{C}$. The optimum temperature for nutrient uptake rates differed among the elements. This was accounted for largely by greater growth at those temperatures of organs with high concentration of particular elements.The $\text{}\text{?}$ga (an estimate of efficiency of uptake) of B varied 12-fold among treatments; K, Ca, Mg, Na, Mn, Zn and Cl varied 3–5- fold and N, P, Cu and Fe varied 2–3-fold.The ratio of relative nutrient accumulation rate, R_m, to the relative growth rate, R_w, was most stable across temperatures for N and least stable for B and Na. However, the trend was for $\text{R}{}_{\mathrm{<mtext>m</mtext>}}\text{R}{}_{\mathrm{<mtext>w</mtext>}}$ to increase as temperature rose, indicating an absorption of nutrients greater than might be expected from a change in growth rate as temperature increased, and a decreased efficiency in their utilization for plant growth.     

Effect of bulb storage temperature on leaf emergence and plant development during scale propagation of Lilium longiflorum ‘White American’

Effects of temperature and duration of bulb storage on leaf emergence of scale bulblets and on the type of plant development were investigated in Lilium longiflorum ‘White American’. After the parent bulbs had been stored for 0, 5, 10 or 15 weeks at 30, 20, 10, or 0°C, scales collected from different parts of a bulb were scale-propagated at 25 or 14°C, or under the temperature regimes normally used in The Netherlands. Independently of the scale position, a higher storage temperature promoted leaf emergence from scale bulblets, whereas a lower temperature delayed it. Higher storage temperatures produced more epigeous type plants, especially from outer and middle scales. Sprouting of the parent bulb had no effect on leaf emergence from the scale bulblets nor on the type of plant development.     

Effects of Temperature on the Growth of Corchorus Olitorius

Controlled environment experiments were conducted using base-line growth study conditions to evaluate the responses of cvs Angbadu and Yaya of Corchorus olitorius, a tropical leafy vegetable, to day/night temperatures of 25°/20°, 30°/25° and 35°/30°C. Some of the parameters studied were greatest at 35°/30°C and least at 25°/20°C for both cultivars. Dry weights of both cultivars increased with rising temperatures during the first 21 days after emergence, and the greatest leaf area was at 30°/25°C for both. The nearest to the optimum temperature regime for this species was 30°/25°C. Based on leaf area at any temperature, cv Yaya would be preferred, but its superiority over cv Angbadu as indicated by dry weight is due to some extent to its greater stem length which is not composed of nutritionally important tissue.     

In vitro morphogenesis and growth of Narcissus in response to temperature

In vitro morphogenesis and growth of tissues of the Narcissus cultivar ‘Lord Nelson’ have been assessed in constant and alternating temperatures. Tissue of leaf-base and scape origin, producing leafy shoots and bulblets on a defined sterile medium, were grown at constant and alternating temperatures of 15, 20, 25 or 30°C and 15–25 or 20–25°C, respectively, in a 16-h daily photoperiod. The number of leaves, leaf length, volume of tissues, fresh weight (but not percentage dry matter), and the number of shoots that would ultimately produce bulbs, were maximal at a constant temperature of 25°C as compared with all other temperature-regimes tested.     

Flowering and fruit-set response to temperature in the avocado cultivar ‘Hass’

Avocado plants, cultivar ‘Hass’, were kept in growth cabinets at 33°C day, 28°C night ($\text{33}\text{28}$); 25°C day, 20°C night ($\text{25}\text{20}$); and 17°C day, 12°C night ($\text{17}\text{12}$), with a 12-h photoperiod and photon flux density of 400 μE m^?2 s^?1 (400–700 nm). At $\text{33}\text{28}$ and $\text{25}\text{20}$ flowers opened in the morning in the female stage and again in the afternoon of the following day in the male stage (Type A floral cycle). At $\text{17}\text{12}$ the flowers opened in the afternoon in the female stage and started to reopen in the male stage 2 days later during the afternoon. They remained open overnight and started to close the following morning. Pollen tube growth and ovule penetration occurred at all temperatures. Pollen tube growth was fastest at $\text{33}\text{28}$, but the pistils had lost the ability to support pollen tube growth by the second week of the experiment. A lower proportion of the ovules at $\text{17}\text{12}$ had an embryosac penetrated by a pollen tube. Embryo development occurred at all temperatures, but fruitlets were abscissed at $\text{33}\text{28}$ and embryo growth was very slow at $\text{17}\text{12}$. The most suitable temperature regime for floral behaviour, pollen tube growth and embryo development was $\text{25}\text{20}$.     

Environmental and cultural effects on vegetative growth and flowering of selected “bedding” ornamentals. II. Night-break lighting and gibberellic acid applications

Weekly foliar applications of $\text{GA}{}_{\mathrm{<mtext>4</mtext><mtext>7</mtext>}}$ (GA; 25 p.p.m.) during seedling growth promoted reproductive development of both Tagetes patula and Matthiola incana whether grown in short days (8 h), or long days effected by night-breaks (NB). The NB treatment was unfavourable for flowering in Tagetes but promoted leaf growth whereas both GA and NB, favourable for early flowering and associated stem elongation in Matthiola, inhibited leaf growth. The GA applications substituted for the long-day requirement for flowering in Matthiola. These findings are coupled with those reported earlier for the effects of night temperature on vegetative growth and flowering and are discussed in relation to a possible “blue-print” for commercial horticultural practice.     

Inhibition of flowering of Mexican- and Guatemalan-type avocados under tropical conditions

Avocado trees of a range of cultivars growing in Darwin, northern Australia (average yearly maximum 33°C, minimum 23°C), were observed for flower and shoot development. Terminal buds of the cultivars ‘Fuerte’, ‘Rincon’ and ‘Edranol’ sampled in July were not floral. Buds which did not burst were sampled in September and they contained developing flowers with perianth primordia. Vegetative extension growth resulted from laterals proximal to the inhibited terminal buds.Avocado trees of the cultivars ‘Fuerte’ and ‘Hass’ which had initiated floral buds were transferred to controlled environment chambers with 33°C day, 23°C night ($\text{33}\text{23}$) or 25°C day, 15°C night ($\text{25}\text{15}$) with a 12-h photoperiod and photon flux density of 400 μmol m^?2 s^?1 (400–700 nm). At $\text{33}\text{23}$ the trees had fewer flowers and a shorter flowering period than at $\text{25}\text{15}$. Inhibited floral buds and lateral vegetative extension resulted at $\text{33}\text{23}$, as observed in northern Australia. The unburst buds had developing flowers with perianth and stamen primordia.The controlled environment experiments showed that the abnormal flushing behaviour of Mexican- and Guatemalan-type avocados growing in northern Australia was due to high temperature. Floral development was inhibited at the stage of stamen differentiation.     

Production protocol development for greenhouse cut Linaria, Lupinus, and Papaver flowers

Linaria maroccana Hook. f. Ann., ‘Lace Violet’, Lupinus hartwegii ssp. cruikshankii Lindl. ‘Sunrise’ and Papaver nudicaule L. ‘Meadow Pastels’ seeds were directly sown into 105 cell plug trays and received either ambient light or supplemental high intensity discharge (HID) lighting. For each species, a 2 × 3 × 3 factorial was used with two light intensities during propagation, three transplant stages, and three night temperatures. Seedlings were transplanted at the appearance of 2–3, 5–6, or 8–9 true leaves. Transplanted Linaria and Papaver seedlings were placed at 5/11, 10/16, or 15/21 ± 1 °C night/day temperatures and Lupinus seedlings were placed at 15/24, 18/25, or 20/26 ± 2 °C night/day temperatures. For this study, the optimum production temperature for Linaria was 10/16 °C as the cut stems produced at 15/21 °C were unmarketable and production time was excessively long at 5/11 °C. At 10/16 °C, Linaria seedlings should be transplanted at the 2–3 leaf stage to maximize stem number, stem length and profitability. For Lupinus the optimum temperature was 15/24 °C due to long stems and high profitability per plant. Lupinus seedlings should be transplanted at the 2–3 leaf stage when grown at 15/24 °C to obtain the longest and thickest stems; however, $/m² week was higher for plants transplanted at the 8–9 leaf stage due to less time in finishing production space. For Papaver, the 15/21 °C temperature was optimal as that temperature produced the longest stems in the shortest duration, resulting in the highest $/m² week. At 15/21 °C Papaver plants should be transplanted at the 2–3 leaf stage. Supplemental HID lighting had no effect on any of the species.     

Effects of temperature and plant growth regulators on anthocyanin synthesis and phenylalanine ammonia-lyase activity in chicory (Cichorium intybus L.)

Summary

This study addresses the effects of air temperature and plant growth regulators on anthocyanin synthesis, sugar content and phenylalanine ammonia-lyase (PAL) activity in chicory (Cichorium intybus L.). Anthocyanin in chicory was synthesised at the highest level under 15°/10°C (day/night) temperatures, followed by 20°/15°C, and 25°/20°C; while synthesis was inhibited > 90% at 30°/25°C, resulting in an almost green colour. Sugar contents paralleled anthocyanin development under the same temperatures. The plant growth regulators, abscisic acid (ABA), ethephon and uniconazole all stimulated anthocyanin synthesis, with uniconazole treatment showing the greatest effect. Gibberellic acid (GA₃) inhibited anthocyanin development, while GA₃ in combination with uniconazole alleviated this inhibition.

PAL activity was higher at 15°/10°C or 20°/15°C (day/night) temperatures when plants were treated with ABA, ethephon or GA₃, than at 25°/20°C and 30°/25°C (day/night) temperatures. These results suggest that, under lower temperatures, plant growth regulators may play an important role in anthocyanin synthesis and PAL activity in chicory.     

Decay of muskmelons stored in controlled atmospheres

Musk melons, Cucumis melo L. var. reticulatus Naud., (commonly referred to as cantaloupes) stored for 14 days in $\text{20\%}\text{CO}{}_2\text{21\%}\text{O}{}_2$ at 5°C plus 3 days in air at 20°C, had less stem scar mold, surface mold, and flesh decay than those held in air or in $\text{5}\text{or}\text{10\%}\text{CO}{}_2\text{21\%}\text{O}{}_2$. Forty percent $\text{CO}{}_2\text{21\%}\text{O}{}_2$ appeared to injure the melons and cause off-odors and off-flavors. Storage in 1, 2, 4 or 8% O₂ for 14 days did not reduce mold growth, as compared with air storage.     

Effects of soil temperature on head development of Cos lettuce

Cos lettuce plants (Lactuca sativa L. cultivar ‘Romana’) of 2 ages were grown for 30 days with soil temperatures of 12°, 20°, 28° or 36°C. The highest temperature caused poor growth and heading and quick head disintegration. Best heading occurred mainly at lower temperature, although the lowest one sometimes delayed growth. Higher temperatures did not enhance real bolting, as growth of the inner stem was accelerated.     

Improvement of caper (Capparis spinosa L.) propagation using in vitro culture and gamma irradiation

Some of the factors influencing the propagation of caper (Capparis spinosa L.) plants in vitro and germination of the seed were studied. The number of adventitious shoots emerging from caper stems cultured in vitro increased from 2.2 shoots per explant when the growth medium contained 2 mg/L of gibberellic acid (GA3) to 5.5 when the growth medium contained 2 mg/L zeatin riboside (ZR) and 1 mg/L naphthalene acetic acid (NAA). The best medium for callus formation from leaf and stem parts contained the growth regulators 1 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L NAA and the best medium for plant regeneration contained 1 mg/L kinetin and 0.1 mg/L indole-3-acetic acid (IAA). The effect of gamma irradiation on the growth of caper shoots in vitro was also studied. A 10 Gy dose of gamma irradiation stimulated growth of shoots up to 200% and increased shoot rooting percentage from 75 to 100%.     

Relationship between bolting-resistance and seed dormancy of different celery cultivars

A correlation between bolting-resistance and seed dormancy was established for 13 celery cultivars. Those cultivars which bolted early in response to a 4-week cold-treatment (7°C) given at the 5-leaf seedling stage had seeds which gave a high germination response to $\text{GA}{}_{\mathrm{<mtext>4</mtext><mtext>7</mtext>}}$ at 2 × 10^?4M in the dark at 22°C. Conversely, bolting-resistant cultivars showed a low germination response to $\text{GA}{}_{\mathrm{<mtext>4</mtext><mtext>7</mtext>}}$. A correlation coefficient (r) of 0.969 was calculated from these data, and values of r = 0.947 and r = 0.950 were obtained using data from field trials on celery bolting in 1974 and 1975, respectively.     

Storage conditions and ripening of the custard apple Annona squamosa L.

Annona squamosa is a climateric fruit in which maximal carbon dioxide production preceeds that of ethylene during post-harvest storage. Normal ripening occurred at temperatures between 15 and 30° C, although the fruits were susceptible to fungal attack at temperatures above 25° C. Storage temperatures below 15° C caused chilling-injuries. Ripening was enhanced by removal of carbon dioxide and by addition of oxygen to the storage atmosphere, and delayed by the addition of carbon dioxide or removal of oxygen. Ethylene had no apparent effect on ripening. Fruits maintained under low relative humidities ripened faster than those stored under high-humidity conditions. Dipping the fruits in a solution of indole acetic acid at concentrations between 10^?4 and 10^?2 M accelerated ripening. Levels of both ascorbic acid and glucose increased to a maximum at the climacteric, but decreased as the fruits became over ripe. The stage of “eating” ripeness occurred at the climacteric. Recommended conditions for storing custard apple are: temperatures between 15 and 20° C, low oxygen and ethylene tensions coupled with 10% carbon dioxide and a relative humidity of 85%–90% in the storage atmosphere.     

Antioxidant and stomatal responses of grapevine (Vitis vinifera L.) to boron toxicity

Although of considerable agronomic importance, our understanding of B toxicity mechanism in plants is still not completely understood, and remains an open question. Therefore, we investigated the effect of increasing levels of B (0, 10, 20 and 30 mg kg⁻¹) on the growth, boron (B) concentrations, stomatal resistance, lipid peroxidation (MDA), membrane permeability (MP), lypoxygenase activity (LOX), proline (PRO) and H₂O₂ accumulation, and the activities of major antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT and ascorbate peroxidase, APX) of grapevine (Vitis vinifera L. cv. Kalecik Karasi) grafted on 5BB rootstock (V. berlandieri × V. riparia) was investigated. Applied toxic levels of B significantly reduced leaf and root growth and increased the B concentration of the leaf, and stem, bark and root of rootstock. In the all B levels leaf tissues of grapevine accumulated more B than that of the other plant parts. In order to restrict excessive uptake of B, stomatal resistance of the leaves increased especially at high B treatments (20 and 30 mg kg⁻¹). The concentrations of H₂O₂, MDA and membrane permeability were increased as the result of B toxicity while proline and the activity of lypoxygenase were decreased. Compared with control plants, the activities of SOD and CAT were increased by B treatments while the activity of APX was decreased. To our knowledge, this is the first report that B toxicity elevated the antioxidant enzymes to protect the membrane functions from reactive oxygen species (ROS) injury in grapevine and it was hoped that this study would provide a basis for developing strategies for reducing the risks associated with B toxicity.     

In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl.

The effects of sodium hypochlorite treatment and growth medium on in vitro seed germination and seedling growth of the leopard orchid (Ansellia africana Lindl.) were investigated. Forty minutes treatment of seeds with 1.75% sodium hypochlorite stimulated germination (70.6%) and seedling development when measured at Stage 5 (emergence of first leaf) on P668 medium after 8 weeks of culture. The growth medium played a significant role in determining the germination response of A. africana seeds. Dark pretreatment of seed cultures significantly enhanced the germination percentage and the growth of rhizoids on the protocorms. Leaf growth in terms of length was substantially enhanced on P668 medium. It was significantly reduced in modified Knudson C medium after 16 weeks of culture. Seedlings developed on P668 medium showed a significantly better growth performance in relation to leaf length, leaf number, root length, fresh and dry weights per plant in vermiculite after 12 weeks of ex vitro growth in a mist house with 90% relative humidity. Further studies developing a suitable method for in vitro symbiotic germination could assist in reintroduction of this threatened orchid species into the wild.