RFLPmapping of a fertility restorer gene for rice (O. sativa L.) ′BT′ type CMS line

The Boro type (BT) cytoplasmic male sterility (CMS) line is the main CMS type for the utilization of heterosis in japonica rice. There is no restorer gene for BT CMS line in japonica rice and it was all from indica rice. The restorer gene used in China is from an IRRI indica variety, IR 8.     

RFLP and SSR mapping of a new gene for brown planthopper resistance introgressed from O. eichingeri into cultivated rice（O.sativa L.）

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Development of Maweizhan cytoplasmic male-sterile line in rice （Oryz.a sativa L. ）

A male sterile mutant plant was found from the rice field of the local rice variety which was cultivated for a long time in the west of Hubei Province. The plant has more vigorous tillering ability. Its plant height of 95 cm was a bit shorter than that of the normal ones.     

Genetic analysis and gene mapping of completely dominant earliness in rice （Oryza sativa L.）

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RFLP tagging of a gene for giant embryo and the integration of chromosome 7 of rice

Lipid content of rice grain was lower than that of corn and other oil crops, but it was rich in the content of unsaturated lopacid. As all the lipid was stored in embryo, one of the promising way to raise its content is to increase the embryo size. Genetic etidies on giant embryo was therefore of great importance. In this experiment, Kinmaze, a japonica giant embryo mutant derived from Japan, and an indica variety Nanjing 11 were used as parents to construct a F_2 population of 58 plants. In combination with eight restriction enzymes (EcoR Ⅰ, EcoR Ⅴ, Dra Ⅰ, Xba Ⅰ, Hind Ⅲ, BamH Ⅰ, Bgl Ⅱ, andSca Ⅰ), 262 DNA clones were used to detect the RFLP's of the parents, of which 73.2% detected polymorphism between the two parents. Seventy-four polymorphism clones were used to analyze the RFLP's of 58 F_2 individuals. Linkage analysis was then performed with the computer package MAPMAKER (Lander et al, 1987). Twelve linkage groups were constructed, among which RFLP markers RG678 and RE395 on chromo     

Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility im rice （Oryza sativa L. ）

The genetic behavior of fertility restoration genes of the cytoplasmic male sterile line Maxie A was studied to facilitate the use of Maweizhan male sterile cytoplasm. The F1,F2, and F1 of Maxie A / Minhui 63 were grown in paddy field, 1993-1995.     

Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice (Oryza sativa L.)

The genetic behavior of fertility restorationgenes of the eytoplasmic male sterile line Maxie A was studied to facilitate the use of Maweizhan male sterile cytoplasm. The F_1,F_2,and F_3 of Maxie A/Minhui 63 were grown in paddy field, 1993-1995. Meanwhile, some of the F_2 plants were testcrossed with the maintainer of Maxie A. The fertilities were     

Identification of a QTL for cold tolerance at early seedling stage via RFLP markers in rice （O. Sativa L. ）

Recently,beneficial genes in japonica and indica varieties were widely used from each other to promote the yield and insect and disease resistance,etc.in rice.Most indica varieties are susceptible to low temperature,and cold injury will affect the rice yield at various growth stages.     

Mapping of QTL for salt tolerance in rice (Oryza sativa L. ) via molecular markers

A recombination inbred (RI) population wasdeveloped by single seed decent from a combi-     

Reproductive versatility of a polyembryonlc line SB-1 of Oryza sativa L

Cytoembryological studies were employed to reveal the origin of haploid and to determine the cytological facters relating to the production of diploid polyembryony within one embryo sac occuring in natural population of a polyembryonic line SB- 1 of O. sativa L. The results showed that one or both synergids may develop into embryos prior to fertilization with frequencies of 2.24% and 1.75% in observed ovaries, respectively. The double fertilization was observed in embryo sacs with one previously-deve-loped multicellular synergid embryo. In caryopsis 3-8 d after anthesis, 1.62% abnormal slender embryos, which are morphologically different from zygotic embryos, were present between the endosperm     

Chromosome location of a gene for aroma in rice

Scented or aromatic rice (Oryza sativa L.) is highly valued in many areas of the world. The development of high yielding scented cultivars has been limited because of lacking information on the inheritance of scent such as linkage of genes for aroma with other genes. We report here our studies on the determination of the chromosomal location of an aroma gene. Twenty-nine marker stocks, including 14     

Identification of a RAPD marker linked to a blast resistance gene in Oryza sativa L.

Marker-aided selection has received more attention in recent years. This relies on the exploitation of close linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAID) marker tightly linked to the blast resistance gene Pi-11(t) derived from Hongjiaozhan, which confers the resistante to race ZBI of Pyricularia oryzae Car.     

A new gamete gene locus ga14 in rice （Oryza sativa L.）

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Regulation of GAs-binding protein on dwarfism of rice （Oryza sativa L.）

The levels of endogenous hormones (GAs and ABA) in rice seedlings (7d) were measured by gas-liquid chromatography with flame ionization detoctor (GLC-FID) and the GAs-binding protein on meanbrane of young rice shoot was also analysed as dascribed by Chiharu.     

STS dataset of major parents in current breeding programs of rice （O.sativa L. subsp.in dica ）

Many major genes of agricultural importance and quantitative trait loci (QTL) controlling yield components in rice have been mapped with molecular markers. The genetic distances between some of the genes and the markers are less than 5cM and the markers are on both flanks of some of the genes, which have laid down the foundation for marker-asslsted selection (MAS) in rice breeding.     

Improvement of foreign gene expression in transgenic rice(Oryza sativa L.)by matrix attachment regions(MARs)

Matrix attachment regions or matrix associated regions (MARs) were special DNA sequences in chromatin of eukaryotic cells that tightly associated with the nuclear matrix or scaffold in vitro after a combination of nuclease digestion and extraction. They were also called scaffold attachment regions(SARs) . It was found that MARs could improve the expression level and the stability of foreign genes in transgenic plants. The reason might be that a transgene flanked by MARs was transmitted into the plant cells, the MARs would attach the nuclear     

Genetic expression and effects of the rolled leaf gene Rl(t) in hybrid rice (Oryza sativa L.)

We constructed a nearisogenic line of rolled leaf gene Rl(t), which ex-pressed incompletely dondnance for the character of rolled leaf(RL), with ge-netic background of Zhenshan 97B. Using RL Zhenshan 97B and the originalZhenshan 97B as the female parents, and Minghui 63 and Yanhui 559 as themale parents, crosses of RL Shanyu 63 (RS63) and Shanyu 63(S63), RLShanyou 559 (RS559) and Shanyou 559 (S559) were made. Inheritance andeffects of Rl(t) in hybrid rice were studied at the flowering and at the 20 d afterflowering, respectively. Results were as follow:     

QTL analysis of rice （O. sativa L.） root vitality in a double halpoid population derived from anther culture of an indica／japonica cross

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