广西:罗汉果育出30亿元大产业

据《广西日报》4月1日有关消息,2018年永福县罗汉果鲜果总产值达9亿多元,加工销售产值20多亿元,与罗汉果相关的农资销售近2亿元,累计产值超过30亿元。永福县是我国传统的罗汉果主产地,1995年,该县就被农业部命名为“中国罗汉果之乡”。2012年开始,该县先后成立永福县罗汉果研究所和广西永福罗汉果试验站,负责罗汉果产业生产、科研、加工、销售、管理、指导,成立了永福县农产品质量安全检验检测站,保障罗汉果绿色安全,并成立永福罗汉果协会,负责罗汉果行业管理。     

广西:桂林举办罗汉果大健康产业落地实施方案编制研讨会

本刊讯继2020年8月桂林罗汉果大健康产业峰会和2020年11月第五届桂林永福罗汉果节暨罗汉果健康产业发展论坛之后,近日,桂林市再次召开桂林罗汉果大健康产业落地实施方案编制研讨会,来自全国各地的专家纷纷为桂林罗汉果发展出谋划策。     

二甲戊灵对罗汉果腋芽生长及药材产量的影响

采用不同浓度(2.75~22 g/L)的二甲戊灵溶液处理罗汉果腋芽,探讨二甲戊灵对罗汉果腋芽生长及药材产量的影响,为罗汉果的轻简化高产高效栽培技术提供指导。结果表明：二甲戊灵能够有效抑制罗汉果腋芽的生长,提高罗汉果特级果和一级果的数量,减少工作量和人工成本,从而提高罗汉果的产值。综合生产成本和单位面积产值,生产上宜选用11 g/L的二甲戊灵溶液处理罗汉果腋芽,以达到最大的经济效益。     

MeJA、SA和ABA对罗汉果苷IIE、III、IV、V和罗汉果醇积累的影响

为了探讨茉莉酸甲酯（Methyl Jasmonate,MeJA）、杨酸（Salicylic Acid, SA）和脱落酸（Abscisic Acid, ABA）对罗汉果苷IIE、III、IV、V和罗汉果醇积累的影响,利用不同浓度的MeJA、SA和ABA喷施人工授粉后50 d的罗汉果果实,诱导48 h后取样,采用高效液相色谱测定罗汉果苷IIE、III、IV、V和罗汉果醇的含量。结果表明,在一定浓度范围内,MeJA、SA和ABA对罗汉果苷IIE、III、IV、V和罗汉果醇的积累均有促进作用。通过掌握MeJA、SA和ABA的调控规律,为提高罗汉果苷IIE、III、IV、V和罗汉果醇的积累奠定了基础。     

广西·桂林举办第二届(中国)罗汉果节

正本刊讯(特约通讯员钱开胜)10月26—27日,2016第二届中国罗汉果节(桂林·永福)在桂林市永福县龙江乡和桂林国际会展中心两地隆重举行。本届罗汉果节由桂林市罗汉果产业联盟、桂林市罗汉果协会、吉福思(中国)罗汉果有限公司等多家单位共同主办。主会场设在桂林市国际会展中心,分会场设在永福县龙江乡。节庆期间举行了2016罗汉果加工新品发布     

广西:永福罗汉果年产果量达10亿个

2018年12月22日,桂林市永福县政府携当地特产永福罗汉果进京,在北京人民大会堂发布“永福罗汉果”区域公用品牌,同时举办了首届罗汉果与肺健康论坛。在发布会上,该县介绍,2018年,永福县罗汉果产业继续在全国保持领先,种植面积稳定在5300hm^2左右,产果量约为10亿个,产值超10亿元。目前该县培育发展了罗汉果加工企业52家,合作社246家,罗汉果种植户达到21328户。     

罗汉果渣的营养特性及其在动物生产中的应用研究进展

罗汉果渣是新鲜罗汉果压榨处理提取甜苷后残存的提取渣,其营养丰富,含有大量粗纤维、多糖及抗氧化物质等。在饲料中添加适量的罗汉果渣,可提高动物的生产性能、机体免疫力及畜产品品质,并且可以节约饲料资源,减轻环境污染。笔者介绍了罗汉果渣的营养特性及其在动物生产中的应用研究进展,旨在为罗汉果渣在动物生产中的合理应用提供参考。     

永福召开执行罗汉果产品国家标准骨干培训会

(特约通讯员钱开胜)据广西永福县农业局信息中心(韦祥和、梁秀容)消息,为了提高永福罗汉果的品质,提升市场竞争力,壮大罗汉果的产业,永福县召开执行罗汉果地理标志产品国家标准骨干培训会。来自全县各地的罗汉果种植、收购、加工及销售专业户和客商以及技术人员参加了会议。     

永福县掀起罗汉果种植热潮

本刊讯（特约通讯员蒙安德）广西壮族自治区永福县的自然条件很适宜种植罗汉果，其单产和总产量均居全国前列。罗汉果具有消暑润肺、化痰止咳、祛热生津、益肝健脾和通便等多种药用功能，近年来国内外市场销售量很大，产品供不应求，果农种罗汉果的效益十分可观。今年永福县在原有的９６７ｈｍ~２罗汉果基础上又扩种了６０ｈｍ~２。该县种植罗汉果最多的是龙江乡，种植面积达到８００ｈｍ~２，占该县罗汉果种植总面积的９０％以上，正常年份产果量达６００万只以上。永福县掀起罗汉果种植热潮@蒙安德     

模拟酸雨胁迫对罗汉果幼苗生理特性的影响

以罗汉果幼苗为材料,研究不同pH值(2.5,3,4,5)及pH 5.6(CK)对罗汉果幼苗丙二醛、可溶性蛋白、叶绿素含量及CAT、POD、SOD活性的影响。结果表明：各模拟酸雨处理罗汉果幼苗中MDA含量随pH值的降低而升高。罗汉果幼苗叶绿素含量、CAT活性随pH值的减小而显著降低。当pH值大于4时,罗汉果幼苗可溶性蛋白含量随pH值的增大而显著增强,罗汉果幼苗体内POD和SOD活性随pH值增大而降低,当pH值小于4时,随pH值的减小罗汉果幼苗可溶性蛋白含量、POD和SOD活性显著降低。当PH值等于4时,罗汉果幼苗可溶性蛋白含量最高,POD和SOD活性最强。CAT、POD、SOD活性与MDA含量呈极显著负相关,可溶性蛋白与这三种酶呈极显著正相关,叶绿素含量与POD、SOD活性呈极显著正相关。     

罗汉果甜苷在酸奶中的代糖作用及其应用前景展望

通过对凝固型酸奶和罗汉果甜苷的论述,阐述了罗汉果甜苷的生理功能和营养价值。传统凝固型酸奶营养丰富但蔗糖含量高、热量高,容易导致肥胖症等疾病的发生,因此,可使用甜味剂替代酸奶中的部分蔗糖。罗汉果甜苷热量低、甜度高又有着良好的保健功能,罗汉果甜苷代糖酸奶具有含糖量少、低热量、健康等优点,易被各类人群尤其是糖尿病人所接受。     

不同活性炭对罗汉果快繁生根的影响

本试验研究不同培养基B5、1/2 B5、MS、1/2 MS以及不同类型、不同浓度活性炭对罗汉果快繁生根的影响。结果表明：不添加活性炭的情况下,四种不同培养基中生根率最高的培养基为B5培养基,生根率为70%,且生根数最多,平均根长最长;粗、细两种活性炭均对罗汉果生根有促进作用,但细活性炭培养基诱导的根更长。在不同类型以及不同浓度活性炭的培养基中生根率最高的为细活性炭1.0 g/L的B5培养基和粗活性炭1.0 g/L的B5培养基,但细活性炭1.0 g/L的B5培养基生根数量和平均根长显著高于(P<0.05)后者。因此,较为适宜的生根培养基配方为：细活性炭1.0g/L,培养基为B5。     

鸡伤寒白痢沙门氏菌培养基和培养方法的研究

本试验用5种培养基对鸡伤寒白痢沙门氏菌（Sgp)菌株C79－20和AS96－1进行了静止培养对比试验，结果表明，改良硫代硫酸钠培养基和EMM培养基为最适合Sgp的培养基，硫代硫酸钠培养基次之，普通营养琼脂和SBG琼脂最差；对C79－13菌株用改良硫代硫酸钠培养基进行了通气搅拌培养、静止厌氧培养和静止需氧培养的对比试验及pH值对细菌生长的影响试验，结果表明，通气搅拌培养方法明显优于其它两种培养方法，调整pH的试验组菌悬液的OD值和菌数明显高于不调整pH值的试验组。     

A simple device for cultivation of anaerobic bacteria (author's transl)]

A new concept for isolation and enumeration of anaerobic bacteria in food is presented. The sample is collected and diluted under anaerobic conditions in a specially designed syringe (M.O.S.), in which cultivation also takes place. A comparison between this method and cultivation in anaerobic jars (GasPak) from a common inoculum revealed a higher number of Clostridium perfringens with the M.O.S.-technique.     

Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland lymphocytes

The aim of this study was to determine whether lymphocyte apoptosis is modulated by infections caused by Staphylococcus aureus and Streptococcus uberis. Samples of cell populations were obtained by lavage of the mammary glands at 4 intervals (24, 48, 72 and 168 h) following infection. The percentage of apoptotic lymphocytes peaked at 168 h after challenge with S. aureus or S. uberis. Subsequent experiments focused on in vitro cultivation of mammary gland lymphocytes with S. aureus and S. uberis. These experiments showed a lower percentage of apoptotic lymphocytes following 3 h of cultivating cells with bacteria than after cultivation without bacteria. The results demonstrate that during both experimental infection of bovine mammary glands with S. aureus or S. uberis and during in vitro cultivation of lymphocytes with S. aureus or S. uberis, apoptosis of lymphocytes is delayed.     

Development of a polymerase chain reaction for the detection of Mycoplasma felis in domestic cats

Mycoplasma felis is associated with conjunctivitis and respiratory disease in domestic cats. Currently no rapid diagnostic test is available for the detection of M. felis in clinical samples that does not rely on prior cultivation of the organism. The objective of this study was to determine whether a polymerase chain reaction (PCR) based upon the 16S/23S rRNA intergenic spacer sequence is suitable for the identification of M. felis directly in feline clinical samples. The high conservation between the 16S/23S rRNA intergenic spacers (IGS) of differing isolates of M. felis was established by sequence analysis and a PCR was developed to this region by comparison to IGS of other mycoplasmas. The PCR was found to be highly specific for M. felis and further PCR analysis on clinical samples showed the PCR to be highly sensitive and more rapid than the other methods of identification currently available.     

Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR

An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7.5 per cent sodium chloride, followed by pcr to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the pcr assay but in only three samples by conventional microbiological culture.     

家蚕悬浮培养细胞系的建立及悬浮培养

通过对家蚕细胞系BmN进行多轮选育 ,选拔出具有较好悬浮性能的细胞株 ,命名为BmN ZJ S ;并在自制转瓶系统中对其进行悬浮培养。实验表明 ,家蚕细胞BmN ZJ S在悬浮培养条件下的细胞扩增倍数为 2 9,最高细胞密度为 4 3 5× 1 0 5个 /mL(细胞活性大于 98%) ,对数生长期延长 2 4h,接近或优于贴壁培养条件下的相应值。     

Evaluation of a nested PCR test and bacterial culture of swabs from the nasal passages and from abscesses in relation to diagnosis of Streptococcus equi infection (strangles)

REASONS FOR PERFORMING STUDY: Streptococcus equi is the cause of strangles in horses. To improve diagnostic sensitivity, development and evaluation of DNA-based methods are necessary. OBJECTIVES: To evaluate diagnostic methods and observe the pattern of bacterial shedding during natural outbreaks. METHODS: Two herds with natural outbreaks of strangles were visited over a period of 15 weeks and 323 samples originating from 35 horses investigated. The diagnostic use of a nested PCR test was evaluated using a collection of 165 isolates of Lancefield group C streptococci (species specificity) and swabs from nasal passages or from abscesses from horses infected with S. equi (diagnostic sensitivity). RESULTS: All 45 S. equi isolates tested positive in the nested PCR, whereas no amplicon was formed when testing the other 120 Lancefield group C isolates. A total of 43 samples were collected from 11 horses showing clinical signs of strangles during the study period. The diagnostic sensitivity for PCR test was 45% and 80% for samples from the nasal passages and abscesses, respectively; the corresponding diagnostic sensitivity for cultivation was 18% and 20%. The diagnostic sensitivity was significantly higher for PCR than for bacterial cultivation. Furthermore, the shedding of S. equi in 2 infected horse populations was evaluated. An intermittent shedding period of S. equi of up to 15 weeks was recorded in this part of the study. It was also shown that shedding of S. equi occurred both from horses with and without clinical signs. CONCLUSIONS AND POTENTIAL RELEVANCE: The nested PCR test represents a species-specific and -sensitive method for diagnosis of S. equi from clinical samples. It may, however, be desirable in future to develop detection methods with high diagnostic sensitivity and specificity without the potential problems inherent in nested PCR.