长期施肥对我国典型土壤活性有机质及碳库管理指数的影响

对我国重点农区的6种典型土壤红壤、灰漠土、垆土、潮土、褐土、黑土长期耕作施肥后的活性有机质及碳库管理指数(CMI)进行了研究,探讨施肥对不同土壤活性有机质和CMI的影响。土壤活性有机质用KMnO4氧化法测定,采用3种浓度KMnO4(33、1673、33.mmol/L)将土壤活性有机质分为高活性有机质、中活性有机质和活性有机质3部分。结果表明,只耕作不施肥(CK)10年后土壤活性有机质含量降低,CMI下降11.1～63.6,其中垆土、褐土下降幅度最大、黑土最小。施用化肥也使土壤活性有机质下降,其中单施氮(N)的潮土活性有机质下降最大,达31.3%;化肥配合施用(NPK)的红壤活性有机质下降最大,其余土壤相对较小。施肥使土壤活性有机质和总有机质含量增加,高于初始土壤和CK。施用有机肥或有机肥配施化肥,土壤活性有机质含量和CMI均显著增加,CMI以红壤上升最大,达91.4,潮土最小,仅为4.6。土壤活性有机质的数量及CMI变幅大于土壤总有机质的变化幅度,以CMI变化为大,说明CMI是评价施肥耕作对土壤质量影响的最好指标。土壤活性有机质分组结果表明,红壤活性有机质组成以高活性有机质为主;垆土、灰漠土活性有机质以高活性和中活性两部分为主;潮土以中活性有机质为主。施肥对红壤、灰漠土活性有机质组分影响明显,对垆土、潮土影响相对较小。     

Chemodestructive fractionation of soil organic matter

The method of chemodestructive fractionation is suggested to assess the composition of soil organic matter. This method is based on determination of the resilience of soil organic matter components and/or different parts of organic compounds to the impact of oxidizing agents. For this purpose, a series of solutions with similar concentration of the oxidant (K₂Cr₂O₇), but with linearly increasing oxidative capacity was prepared. Chemodestructive fractionation showed that the portion of easily oxidizable (labile) organic matter in humus horizons of different soil types depends on the conditions of soil formation. It was maximal in hydromorphic soils of the taiga zone and minimal in automorphic soils of the dry steppe zone. The portion of easily oxidizable organic matter in arable soils increased with an increase in the rate of organic fertilizers application. The long-lasting agricultural use of soils and burying of the humus horizons within the upper one-meter layer resulted in the decreasing content of easily oxidizable organic matter. It was found that the portion of easily oxidizable organic matter decreases by the mid-summer or fall in comparison with the spring or early summer period.     

Labile pools of organic matter and microbial biomass in the surface soils under shifting cultivation in northern Thailand

In order to analyze the N mineralization process under shifting cultivation in northern Thailand, labile pools of soil organic matter were studied, which were considered to be the factors contributing to the N mineralization process. Organic C, (organic + NH₄ ⁺)-N, and hexose-C were extracted from fresh soils in the surface 0–5 cm layers with a 0.5 M K₂S₀. solution at 110°C in an autoclave (fraction A) or at room temperature with a reciprocal shaker (fraction B), and analyzed as labile pools of organic matter. In the traditional shifting cultivation system, the content of organic C in fraction A in the fallow fields for 8 to 15 y was 3,710 mg kg^-1 while that in the fallow fields for 1 y and 3 to 5 y was 2,640 and 2,600 mg kg^-1, respectively. A high correlation was observed between the contents of the labile pool in fraction A and total soil organic matter. The ratio of the pool in fraction A to total soil organic matter apparently remained constant through the input-output balance in the pool. The content of the labile pool in fraction B was the highest among the fields cultivated for 1 y after the slash and burn practice and it decreased in the course of the fallow period. The content of organic C was 548 mg kg^-1 in the fields cultivated for 1 y and 235 mg kg^-1 in the fallow fields for 8-15 y, respectively. There was a reverse relation between the contents of the pool in fraction B and microbial biomass. Therefore, the origin of the pool in fraction B was attributed to the microbial debris associated mainly with a decrease in the soil moisture content in the dry season. On the other hand, in the relatively intensive cultivation system, there was no significant difference in the contents of the labile pools both in fractions A and B among the land use stages, suggesting that the preservation mechanism of these pools, which was observed in the traditional cultivation system, did not operate well in the intensive system. In alternative farming systems in future, it will be essential to apply organic materials to soils to supply organic matter and to maintain the microbial biomass.     

The effect of geocryological conditions and soil properties on the spatial variation in the CO<Subscript>2</Subscript> emission from flat-topped peat mounds in the isolated permafrost zone of Western Siberia

The active layer thickness, CO2 emission, and contents of organic substances (including the total organic carbon, labile carbon, and the carbon of microbial biomass) in the soils of flat-topped peat mounds in the area of the Nadym Experimental Station in the north of Western Siberia (experimental site CALM R1) are characterized by considerable spatial variability. The low values of the CО₂ emission are confined to the microelevations on the peatland surface. The high values of the emission (>200 mg CO₂/(m² h)) are typical of the soils with the highest content of the carbon of microbial biomass and the lowest content of the labile organic carbon. The soils of elevated flat-topped peat mounds statistically differ from the soils of waterlogged mires in the contents of total, labile, and microbial carbon and in the CO₂ emission values. Though the soils of elevated flat-topped peat mounds are characterized by the high content of the carbon of microbial biomass (4260 ± 880 mg С/kg soil), the CO₂ emission from them is low (158 ± 23 mg CO₂/(m² h)), which is explained by the structure of microbial communities in the cryogenic soils and by the effect of specific hydrothermic conditions.     

Factors Affecting Microbial Biomass and Dehydrogenasc Activity in Apple Orchard Soils with Heavy Metal Accumulation

The size of the microbial biomass and dehydrogenase activity were measured in air-dried and rewetted apple orchard surface soils with accumulation of Cu, Pb, and As due to the application of Bordeaux mixtures and lead arsenate. The largest amounts of total Cu, Pb, and As found in the soils used were 1,108, 1,271, and 209 mg kg^-1 soil, respectively. The amounts of 0.1 M HCl-extractable heavy metals were strongly correlated with the total amounts, while those of 0.1 M CaCl₂-extractable heavy metals, except for As, increased significantly with decreasing soil pH. The amounts of microbial biomass C and N, expressed on a soil organic C and total N basis, respectively, were each negatively correlated with the amounts of total and 0.1 M HCl-extractable Cu. On the other hand, the dehydrogenase activity was not affected by the amounts of total and 0.1 M HCl-extractable heavy metals, and was negatively correlated with the amount of 0.1 M CaCl₂-extractable Cu and positively with the soil pH. Higher significant correlations were observed when the dehydrogenase activity was calculated per unit of soil organic C. Thus the microbial biomass was adversely affected by the slightly soluble fractions of Cu accumulated in apple orchard soils, whereas the dehydrogenase activity was affected by the water-soluble and exchangeable Cu of which amount depended on the soil pH. It is suggested that the microbial biomass and dehydrogenase activity expressed on a soil organic matter basis could become useful indicators for assessing the effects of heavy metals on the size and activity of the microbial biomass in soils differing in organic matter contents.     

Einfluß der Bodeneigenschaften auf die Freisetzung der gelösten organischen Substanz (DOM) aus dem Oberboden

Influence of Soil Properties on the Release of Dissolved Organic Matter (DOM) from the Topsoil A percolation experiment over a period of three month with small monoliths from forest and grassland soils varying in their anthropogenic changes was carried out to identify, to weigh and to quantify important soil properties for DOM release from the topsoil. Quality of soil organic matter determines the amount of DOM released from the topsoil if the soils have a low ability to adsorb and to precipitate DOM. Favorable conditions for high DOC concentrations in the soil solution are wide C/N ratios in the soil and in the hot water soluble fraction, a high soil content of hot water soluble organic carbon and a high portion of hot water soluble organic carbon in the total organic carbon content. Anthropogenic changes of the soils are effective to DOM release via changing quality of soil organic matter. Long dry periods and large water fluxes can further increase DOM release. The effects of soil organic matter can be disguised in soils with a high DOM retention capacity (high CEC, high content of exchangeable bases, Fe_ox and Fe_d). Then adsorption and precipitation determine DOM release from the topsoil and contribute to a decrease of DOM release.     

Soil organic matter alteration under biochar amendment: study in the incubation experiment on the Podzol soils of the Leningrad region (Russia)

Purpose

Biochar is one of the most widely used ameliorants for soil amendment, which is known as factor which rises crop yields and levels of soil biological activity. Nowadays, it is under investigated how biochar application affects the dynamics of the humic components and whole soil organic matter (SOM) and the processes of its alteration. This investigation is aimed to evaluate the influence of biochar on the content, composition, and transformation of humic acids (HAs) as the main component of the SOM.

Materials and methods

The incubation experiment was carried out on three Podzol Antric soils, with varying amounts of initial total organic carbon. The incubation time was 90 days, using biochar gravimetric doses of 0.1 and 1.0%. The biochar was produced by fast pyrolysis of birch and aspen wood at 550 °С. Humus composition was analyzed for the organic matter fractions extracted with 0.1 M NaOH (containing HAs 1 + fulvic acids (FAs) 1) and 0.1 M Na₄P₂O₇ (containing HAs 1 + FAs 1 + HAs 2 + FAs 2). Isolated HAs were characterized for their elemental composition (C, N, H, and S) and molecular composition with the use of solid-state 13C nuclear magnetic resonance (13C-NMR) techniques.

Results and discussion

We found that 0.1% of biochar amendment does not influence SOM mineralization, but 1.0% of biochar increases the mineralization by 15–18%. This process is accompanied by changes in the composition and properties of the HS. The increased proportion of HA aromatic fragments in biochar indicates an increasing of their stability. However, in soils with high humus content and a significant amount of insoluble matter, the processes of mineralization and the growth of HAs are taking place simultaneously. The replenishment of HAs could be the outcome of both the intensification of the transformation processes (mineralization and humification) of the more sustainable insoluble matter compounds and the humification of the biochar itself.

Conclusions

The influence of biochar on humification in Podzol Antric soils was revealed on the basis of incubation experiment. Both negative and positive changes under biochar in HS system were demonstrated. The active decrease of humus total contents and also the labile HS ought to qualify as negative changes. The increase of HA chemical maturity that leads to the stability of humus in whole as well as the intensive new HA formation thought to qualify as positive changes.

     

Solubility of the labile forms of soil carbon and nitrogen in K2SO4 of different concentrations

The general pattern of the changes in the solubility of the labile carbon and nitrogen compounds with the changes in the concentration of the salt extractant (0.05 and 0.5 M K₂SO₄) has been determined for soils differing in their acidity and in their contents of organic matter and nitrogen. Different forms of extracted compounds react differently to changes in the salt concentration. The solubility of inorganic nitrogen compounds (NH ₄ ⁺ and NO ₃ ^? ) does not depend on the concentration of K₂SO₄. In most cases, the carbon and nitrogen of the microbial biomass manifest a tendency for increasing extractability with an increase in the concentration of the K₂SO₄ solution. A fundamental difference is characteristic of the organic carbon and nitrogen compounds, the solubility of which in 0.5 M K₂SO₄ increases in different soils by 1.5–3.9 times in comparison with their solubility in 0.05 M K₂SO₄.     

Effect of the storage conditions of soil samples on carbon and nitrogen extractability

Concentrations of carbon and nitrogen extractable by 0.05 M K₂SO₄ (C_ext and N_ext, respectively) in 14 soils of different ecosystems vary from 16 to 205 and from 4 to 53 mg/kg, respectively. The portion of C_ext in soil organic matter is 0.06 to 0.38% of total carbon, and the portion of N_ext is 0.12–1.05% of total nitrogen. The storage of samples and their preparation to analysis differently affect the extractability of elements. The concentration of C_ext is less variable than the concentration of N_ext. An increase in C extractability (by 1.4–6.7 times) is a common feature of all soils under drying; at the following incubation of dried soils, the extractability of C decreases by 28–56%. The extractability of N increases not only under drying (by 1.5–7.1 times) and the following incubation of samples (by 25–60% to 2–3 times), but also under freezing of most soils and at the incubation of fresh and defrozen samples. A close direct correlation is observed between the initial water content of soil and the relative increase in C extractability under drying and N extractability under freezing and drying.     

Soluble selenium content of agricultural soils in Japan and its determining factors with reference to soil type,land use and region

Abstract

To evaluate labile selenium (Se) content in agricultural soils in Japan and to investigate its determining factors, 178 soil samples were collected from the surface layer of paddy or upland fields in Japan and their soluble Se contents were determined. Two grams of soil was extracted with 20 mL of 0.1 mol L^?1 sodium sulfate (Na₂SO₄) solution for 30 min in boiling water, and the released Se was reduced to Se (IV) after organic matter decomposition. The concentration of Se (IV) was then determined by high performance liquid chromatography (HPLC) with a fluorescence detector after treatment with 2,3-diaminonaphthalene (DAN) and extraction with cyclohexane. Soluble Se content ranged from 2.5 to 44.5 μg kg^?1 with geometric and arithmetic means of 11.4 and 12.8 μg kg^?1, respectively, and corresponded to 3.2% of the total Se on average. The overall data showed log-normal distribution. In terms of soil type, Non-allophanic Andosols and Volcanogenous Regosols had relatively high soluble Se content, and Wet Andosols and Lowland Paddy soils had relatively low soluble Se content. In terms of land use, upland soils had significantly higher soluble Se content than paddy soils (p < 0.01). The soluble Se content had significant positive correlation with total organic carbon (TOC) content of the extract, soil pH and total Se content (p < 0.01). In conclusion, total Se content in combination with soil pH was the main determining factor of the soluble Se content of agricultural soils in Japan.     

Effect of crop residues on the organic matter composition of a leached chernozem in the Western Siberian forest-steppe

In leached chernozems used in crop rotations with different amounts of plant residues for nine years, the following parameters have been determined: the changes in the contents of the total carbon and carbon and nitrogen from the readily hydrolyzable components of the soil organic matter: the labile humus, detritus, and mortmass. No significant differences in the content of C_org in the soil among the crop rotations have been found. The different inputs of plant residues have significantly affected the contents of carbon and nitrogen in the readily mineralizable soil organic matter. The decrease in the mean annual input of the aboveground plant residues to the soil from 1.5 to 0.2 t C/ha resulted in the reduction of the carbon and nitrogen contents in the soil by 19–25% for the labile humus, 24–28% for the detritus, and 33–36% for the mortmass. The labile humus formed the largest fraction (3890 mg of C/kg soil or 10.3% of C_org on the average for the crop rotations); the fractions of the detritus (1546 mg C/kg soil or 10.3%) and mortmass (627 mg C/kg soil or 1.7% of C_org) were the next.     

Translocation of soils to stimulate climate change: CO2 emissions and modifications to soil organic matter

The effect of climate change on CO₂ emissions was studied on undisturbed soil monoliths (40‐cm diameter, 25‐cm high), which were translocated to warmer zones than their place of origin. Thirty‐two months after the translocation, a climatic factor deduced from the moisture content of the soil and from the effective mean temperature (temperatures in excess of 5°C) revealed that translocation increased the potential of the climate to enhance the biological processes by between 73% and 26% compared with what the soil would support in its place of origin. At the end of the study, the transported soils had lost a large proportion of both total carbon and nitrogen (between 20 and 45%). During the experiment, the CO₂ emissions from the soils, measured under field conditions, were quite variable, but were usually greater than from soils in situ. The variation in labile C in the soil throughout the experiment was calculated from a first‐order kinetic equation for organic matter decay. The relative CO₂ emissions, expressed in terms of the labile carbon fraction in the soils, were clearly greater in those translocated soils that underwent the most intensive climate change, which indicates that the variations in emissions over time are basically a function of the size of the labile organic matter pool.     

Water stability of soil aggregates and their ability to sequester carbon in soils of vineyards in Slovakia

Soil water-stable aggregation is an important process for carbon sequestration and is a key factor controlling soil sustainability and resilience; therefore, the objectives of the present study were to (1) evaluate the differences in soil organic matter state, its specific and labile fractions and their importance in the formation of water-stable aggregates in vineyard soils differing in their genesis and texture under different soil management (vineyard rows – tilled and grassed in-between strips), and (2) estimate the ability of the vineyard soils to sequester soil organic carbon (SOC) into water-stable macro-aggregates (WSA_ma). The results showed that the WSA_ma content of the soils ranged from 47% to 97%. Soils with grasses had a higher SOC and labile carbon (C_L) contents than the bulk soil and, as a result, the higher total WSA_ma content. Soils ranged in a decreasing order in their ability to sequester SOC and C_L from bulk soil to WSA_ma: Haplic and Stagni-Haplic Luvisols > Calcaric Fluvisol = Rendzic Leptosol > Haplic and Luvi-Haplic Chernozem > Dystric and Eutric Cambisols. Our results showed that the maximum ratio of SOC content in WSA_ma to that in bulk soil was 1.0 at the maximum WSA_ma content regardless of the soil type. An increase in the ratio above this threshold value (1.0) resulted in a decrease in WSA_ma content.     

Carbon mineralization rates at different soil depths across a network of European forest sites (FORCAST)

Most of the carbon (C) in terrestrial ecosystems is stored in the mineral soil layers. Thus, the response of the mineral soil to potential increases in temperature is crucial for the prediction of the impact of climate change on terrestrial ecosystems. Samples from three mineral soil layers were collected from eight mature forest sites in the European network CARBOEUROFLUX and were incubated at four temperatures (4, 10, 20 and 30°C) for c. 270 days. Carbon mineralization rates were related to soil and site characteristics. Soil water holding capacity, C content, nitrogen (N) content and organic matter all decreased with soil depth at all sites, with significantly larger amounts of organic matter, C and N in the top 0–5 cm of mineral soil than in the deeper layers. The conifer forest soils had significantly lower pH, higher C/N ratios and carbon contents in the top 5 cm than the broadleaf forest soils. Carbon mineralization rates decreased with soil depth and time at all sites but increased with temperature, with the highest rates measured at 30°C for all sites. Between 50 and 70% of the total C respired after 270 days of incubation came from the top 5 cm. The percentage C loss was small in all cases, ranging from 1 to 10%. A two‐compartment model was fitted to all data to derive the labile/active and slow/recalcitrant fractions, as well as their decomposition constants. Although the labile fraction was small in all cases, we found significantly larger amounts of labile C in the broadleaf forest soils than in the conifer forest soils. No statistically significant differences were found in the temperature sensitivity parameter Q₁₀ among sites, soil layers or between conifer and broadleaf soils. The average Q₁₀ for all soils was 2.98 (± 0.10). We found that despite large differences among sites, C mineralization can be successfully predicted as a combined function of site leaf area index, mean annual temperature and content of labile carbon in the soil (R² = 0.93).     

不同浸提剂处理森林土壤溶解性有机碳含量比较

为了解亚热带森林土壤溶解性有机碳(DOC)的特征规律,采用培养离心的方法获取土壤溶液测得DOC含量,对比传统水溶性有机碳(WSOC)提取法间的差异。选取湖南大山冲森林公园保存完好的3种亚热带典型次生林地,按10cm一层采集剖面土壤,采用不同方法提取测定土壤DOC和WSOC含量,分析与土壤理化指标的相关性及方法间的显著性关系。结果表明:①典型森林土壤DOC或WSOC含量随土壤剖面深度的增加,呈显著下降趋势。培养离心提取测得的土壤DOC含量明显较低,仅0.82～9.52 mg/kg,超纯水浸提的风干土WSOC含量达10.56～249.19 mg/kg,而0.5 mol/L K₂SO₄提取的鲜土WSOC含量达155.70～576.94 mg/kg,0.5mol/L K₂SO₄浸提的干土WSOC含量最高,达158.94～797.56 mg/kg,含量表现为:DOC<干土超纯水浸提WSOC<鲜土K₂SO₄浸提WSOC<干土K₂...     

不同施肥制度下我国东部典型土壤易分解与耐分解碳的组分特征

【目的】土壤易分解碳库(labile organic carbon,Lab-C)和耐分解碳库(recalcitrant organic carbon,Rec-C)是土壤有机质的重要组分,其组分大小与比例可反映土壤有机碳的周转与固存特性。因此,研究长期不同施肥制度下土壤易分解碳库与耐分解碳库的大小与比例,对土壤养分管理及肥力培育具有重要的意义。【方法】利用我国东部23年长期不同施肥制度下的黑土、潮土、红壤和32年水稻土共四类土壤的典型土样为代表,以不施肥(CK)、施化肥(NPK)、化肥配施秸秆(NPKS)和化肥配施有机肥(NPKM)4个处理土壤,采用颗粒密度相结合的方法,将土壤有机碳分为易分解碳和耐分解碳2个组分,分析了其不同组分碳含量及比例的变化特征。【结果】土壤经该方法分组后,四种土壤的平均质量回收率和碳回收率均超过95%,是一种测定土壤易分解碳和耐分解碳的可行方法。旱作土壤(黑土、潮土和红壤)易分解碳的平均含量为1.91 g/kg低于水田的2.42 g/kg,而易分解碳占总有机碳的平均比例为15.4%,高于水田的9.9%。NPKM处理下,黑土、潮土和红壤易分解碳含量显著高于NPKS、NPK及CK处理(P0.05),较NPK处理增加的比例分别为98.4%、43.7%和71.2%,同时提高了易分解碳占总有机碳的比例,但无显著差异性;NPK和NPKS处理下黑土与潮土易分解碳的含量较不施肥无显著变化,而红壤易分解碳含量较不施肥显著降低(P0.05),降低的比例分别为33.1%和29.6%;水稻土4个处理间易分解碳的含量及其占全碳的比例无显著差异性。四类土壤耐分解碳的含量与总有机碳含量的变化一致,均表现为NPKMNPKSNPKCK。NPKM处理下,四种土壤耐分解碳含量显著增加(P0.05),黑土、潮土、红壤和水稻土较NPK处理增加的比例分别为68.8%、42.7%、17.6%和17.2%,同时耐分解碳占全碳的比例降低;NPKS处理下黑土、潮土和水稻土耐分解碳的含量较NPK处理也增加,对应增加的比例分别为10.9%、15.1%和18.0%。同时,易分解碳和耐分解碳的含量与土壤总有机碳含量之间有极显著的正相关关系。【结论】旱作土壤易分解碳含量比水田土壤更易受不同施肥处理的影响,有机无机配施(NPKM与NPKS)可提高旱作与水田土壤易分解碳与耐分解碳的含量,同时相对提高了易分解碳占全碳的比例,且NPKM处理的效果优于NPKS处理,更优于化肥处理。     

Origin and properties of humus in the subsoil of irrigated rice paddies

Stability of humus in the plow layer soil is considered to affect the quantity and quality of dissolved organic matter leached from the plow layer soil. Therefore, a model experiment was conducted to analyze the effect of soil reduction under submerged conditions on the stability of humus in the plow layer soil. The changes in the stability of humus in the plow layer soil during submerged incubations with and without rice straw application were evaluated based on the changes in the binding type of humus. Binding type of humus in the plow layer soil was analyzed by successive extractions of organic matter with water, 0.25 M Na₂SO₄, 0.1 M Na₄P₂O₇ (pH 7.0), 0.1 M Na₄P₂O₇ (pH 10.5), and 0.1 M Na₄P₂O₇ (pH 10.5) with NaBH₄. Amounts of Fe, Mn, and Mg in each fraction were also determined to estimate the relationships between humus and metals.

The successive extraction of humus indicated that the amount of organic carbon which was extractable with the (NaBH₄ +0.1 M Na₄P₂O₇) solution decreased while that of the 0.1 M Na₄P₂O₇ (pH 7.0}-extractable organic carbon increased during submerged incubation with rice straw application. The origin of the increase in the amount of organic carbon in the Na₄P₂O₇ (pH 7.0)-extractable fraction during submerged incubation was investigated further by another incubation experiment using ¹³C-glucose as a reducing agent. Atom- 13C% analysis showed that the contribution of organic carbon derived from compounds other than glucose to the increase in the contents of humic acids and fulvic acids in the Na₄P₂O₇ (pH 7.0)-extractable fractions was ca. 80%. Therefore, it was concluded that the binding type of humus changed from (NaBH₄ + Na₄P₂O₇)-extractable to Na₄P₂O₇ (pH 7.0)-extractable humus under reducing conditions. Since the amounts of organic carbon and Fe increased in the Na₄P₂O₇ (pH 7.0)-extractable fraction and decreased in the (NaBH₄ +0.1 M Na₄P₂O₇)-extractable fraction simultaneously, iron reduction was presumably associated with the change in the binding type of humus in submerged paddy soil.     

Factors controlling mineralization of soil organic matter in the Eurasian steppe

To understand the dynamics of soil organic matter (SOM) in the Eurasian steppe, several soil and meteorological properties were tested in order to estimate the amounts of potentially mineralizable organic carbon (PMC) and nitrogen (PMN). Total 41 surface soil samples were collected in Ukraine and Kazakhstan from cropland, forest, grassland, and desert ecosystems. The fresh soils were incubated for 133 days under constant temperature and moisture conditions, and the CO₂ emissions and the mineral N from the soils were monitored. PMC and PMN were determined by fitting models to the cumulative curves of the CO₂ and the mineral N. Tested soil properties included soil pH, sand, silt and clay contents, carbon and nitrogen contents of light fraction (LF, <1.6 g cm^?3) and heavy fraction (HF), and C/N ratio of LF and HF. The meteorological properties considered were mean annual temperature and precipitation. Using multiple regression analysis with the stepwise method, PMC was well estimated by carbon content of LF (LFC) and clay content, compared to the simple correlation with organic carbon (OC). Similarly, PMN was better determined by nitrogen content of LF (LFN) and clay content. These results suggest the partially labile nature of clay-associating OM and of LFC and LFN. The higher PMC and PMN in the forest and grassland sites would be attributed to the higher LFC and LFN, while the lower LFC and LFN in cropland sites would suggest the relatively higher contribution of clay-associating OM to PMC and PMN.     

长期施肥和秸秆覆盖土壤活性有机质及碳库管理指数变化

【目的】研究长期施肥和秸秆覆盖对土壤活性有机质和碳库管理指数的影响,为渭北旱塬区秸秆覆盖模式的完善提供理论依据。 【方法】长期定位试验于1981年开始,布设于陕西省合阳县,设空白对照（CK）、氮磷肥配施（NP）、氮磷有机肥配施（NPM）、氮磷肥配施+秸秆覆盖（NP+FG）、氮磷有机肥配施+秸秆覆盖（NPM+FG）5个处理,测定28年后土壤有机质、不同活性组分有机质含量及土壤养分含量、酶活性,研究长期施肥和秸秆覆盖对土壤活性有机质、碳库管理指数的影响及其与土壤酶活性、养分含量的相关关系。 【结果】1）NP处理较CK处理显著提高了土壤高活性有机质含量48.7%,对其他活性组分影响不显著;NPM处理土壤总有机质含量提高了120.0%,高、中、低活性有机质含量分别提高了137.4%、136.3%和93.4%。2）以CK为参考土壤,NP处理土壤高活性、中活性和活性碳库管理指数分别提高了54.66、17.93和2.65;NPM处理分别提高了139.28、140.92和83.59。NP+FG处理土壤高、中、低活性碳库管理指数较NP处理分别提高75.01、191.43和122.90;NPM+FG处理较NPM处理分别提高了58.93、121.35和93.43。3）高活性有机质、高活性碳库管理指数与转化酶呈极显著相关,中、低活性碳库管理指数与转化酶呈显著相关。 【结论】NPM处理显著提高了土壤高、中、活性碳库管理指数;施肥基础上秸秆覆盖进一步提高了土壤的碳库管理指数,在氮磷肥配施基础上进行秸秆覆盖对碳库管理指数的提升作用大于氮磷有机肥配施基础上秸秆覆盖的作用;活性有机质与总有机质相比,更能反映土壤酶活性的变化,有机质的活性越高,对指示酶活性和土壤质量的变化越敏感。在渭北旱塬区,氮磷肥基础上秸秆覆盖表现出显著提高土壤碳库管理指数和培肥的优势。     

Assessing soil carbon lability by near infrared spectroscopy and NaOCl oxidation

The feasibility of near infrared (NIR) spectroscopy for quantifying labile organic matter (OM) in arable soils and for predicting soil refractory OM fractions was tested on 37 soils varying in texture and soil carbon (C) content. Three sets of arable soils (0-20 cm depth) were sampled from 1) long-term field experiments with different OM inputs, 2) individual sites with inherent with-in field gradients in soil texture and/or C content, and 3) from a range of different sites covering variations in management and geological origin. The labile OM fraction was defined by the CO₂ evolved from the soils incubated for 34 weeks while refractory OM was obtained by NaOCl oxidation.The labile fraction of the soil C accounted for 2-12% of the total soil C content. No systematic relationship between labile C content and total soil C or clay was found, but NIR spectra could be correlated well with the labile C fraction. A distinct, close linear relationship was found for C in soil before and after the NaOCl oxidation, indicating that this method was unable to reveal any additional information not contained in the total soil C measurement. NIR was also correlated with the NaOCl resistant C fraction, but this was considered to relate to the ability of NIR to predict total soil C contents. Thus NIR seemed to have the potential to estimate labile OM determined under laboratory incubations, while it still remains open how to identify and quantify refractory pools of soil OM.