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樟油的提取及其抑菌性研究 总被引:2,自引:0,他引:2
通过蒸馏法从樟树的树根、树枝、鲜叶及落叶中提取樟油,然后将用蒸馏法从落叶中获得的樟油和用丙酮浸提法从落叶中获得的提取物分别配制成不同浓度的水溶液,采用抑菌圈法分别对常见的几种细菌进行抑菌研究,结果表明落叶中的得油率最高,用蒸馏法获得的樟油对大肠杆菌(Escherichia coli)、普通变形杆菌(Proteus vulgaris)、金黄葡萄球菌(Staphylococcus aureus)有一定的抑菌作用,其最低抑菌浓度(MIC)在0.125~0.25 g.mL-1之间,而丙酮浸提液对上述细菌的生长几乎没有抑制作用。 相似文献
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以竹园采集土样分离出的根际微生物为主要研究对象,采用16s rDNA分子生物学手段和常规生理生化分析方法,对笋用竹正常生长植株及病害植株进行分析研究。有病竹植株的根际微生物可培养菌落数目比正常竹植株的多。特征分析表明:在分离纯化出的45株细菌中,其中的8株细菌初步鉴定为:菌株5-1-4为地衣芽孢杆菌Bacillus licheniformis,菌株5-1-10为苏云金芽孢杆菌Bacillus thuringiensis,4-1-17为枯草芽孢杆菌Bacillus subti-lis,菌株3-1-8为洋葱博克霍尔德菌Burkholderia cepacia,菌株2-1-9为粘金黄菌Chryseobacterium gleum,菌株4-1-14为短小芽孢杆菌Bacillus pumilus,菌株4-1-16为普通变形菌Proteus vulgaris,菌株4-1-6为产气肠杆菌Enterobacter aerogenes。 相似文献
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回顾了我国有关红色旅游、红色旅游共生及红色旅游绩效的相关研究并进行了综述。根据文献对相关概念进行了整理,根据研究成果的数量和内容对红色旅游相关研究进行了分析,提出了各发展阶段及各阶段内容特征。对旅游共生的研究内容进行了整理,对红色旅游研究进行了总结,得出了现阶段的发展和未来的展望。 相似文献
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对枫香进行了多年的播种育苗试验。对其苗期苗高与地径生长进行了定株定期观测。在此基础上 ,对其苗期生长节律、苗高生长与气象因子的关系进行了分析 ,并对主要育苗技术措施进行了总结。 相似文献
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文章就锡林郭勒盟乌拉盖湖旅游资源现状进行了分析,对旅游资源进行了评价,同时对该地区发展旅游业的机遇和面临的挑战进行了论述,对发展旅游业给区域生态环境造成的破坏也进行了客观的论述。 相似文献
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The ethanolic extract of Gymnema sylvestre leaves demonstrated antimicrobial activity against Bacillus pumilis, B. subtilis, Pseudomonas aeruginosa and Staphylococcus aureus and inactivity against Proteus vulgaris and Escherichia coli. 相似文献
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The ethanolic extracts of the dry fruits of Caesalpinia pulcherrima, aerial parts of Euphorbia hirta and flowers of Asystasia gangeticum were tested for antimicrobial activity. The three plants exhibited a broad spectrum of antimicrobial activity, particularly against Escherichia coli (enteropathogen), Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus. 相似文献
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The ethanolic extracts of the leaves and flowers of Cleome viscosa and roots of Gmelina asiatica were tested for antimicrobial activity. The two plants exhibited a broad spectrum of antimicrobial activity, particularly significative against Escherichia coli , Proteus vulgaris and Pseudomonas aeruginosa. The leaf extract of C. viscosa showed moderate activity against pathogenic fungi. 相似文献
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Antibacterial and molluscicidal activities of the essential oil of Chrysanthemum viscidehirtum. 总被引:3,自引:0,他引:3
F Khallouki M Hmamouchi C Younos R Soulimani J M Bessiere E M Essassi 《Fitoterapia》2000,71(5):544-546
The volatile fraction of Chrysanthemum viscidehirtum aerial parts, consisting mainly of limonene, beta-farnesene and many oxygenated sesquiterpenes, was screened for activity against 21 microbial strains. This essential oil exhibited activity against all germs tested, in particular Salmonella typhi and Proteus mirabilis. It also showed molluscicidal activity against Bulinus truncatus. 相似文献
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Lythrum salicaria extracts showed activity against the phytopathogenic fungus Cladosporium cucumerinum and activity against the bacteria Staphylococcus aureus, Proteus mirabilis and Microccocus luteus. Bioautography on thin-layer chromatograms was used to isolate the two antifungal triterpenoids oleanolic and ursolic acid. The hexahydroxydiphenoyl ester vescalagin was isolated as active principle of the antibacterial activity. Furthermore, the flavon-C-glucosides vitexin, isovitexin, orientin and isoorientin were isolated. 相似文献
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The β-carboline alkaloids of Peganum harmala L were extracted through a bioassay-guided fractionation and their antimicrobial activities were investigated. Results revealed significant differences (P > 0.05) between compounds depending on the microorganism tested and the application method. When examined individually, harmine was the most effective against Proteus vulgaris, Bacillus subtilis and Candida albicans where inhibition zones ranged between 21.2 and 24.7 mm. Potentiality of the alkaloids was increased when applied as binary mixtures suggesting a kind of synergistic interaction with inhibition zones reaching 31.5 mm with the total alkaloidal extract. We recommended the use of such compounds as new antimicrobial biorationals. 相似文献
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以广东紫珠药材粉末为原料,探讨其不同提取部位的抑菌作用,并筛选其有效部位。对广东紫珠药材用不同极性大小溶剂(石油醚、二氯甲烷、乙酸乙酯、乙醇、70%乙醇、水)回流提取,获得广东紫珠粗提物,用滤纸片法测定其抑菌圈大小(金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌、白色念珠菌),对抑菌活性明显的提取部位进行梯度萃取,并分别测定其抑菌圈大小,得到广东紫珠抑菌有效部位,并测定其有抑菌作用的有效部位的最小抑菌浓度。结果显示广东紫珠70%乙醇提取物对金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌均表现了不同程度的抑菌活性,初步判定为广东紫珠的抑菌活性部位,70%广东紫珠提取物经过萃取后,石油醚萃取物及二氯甲烷萃取物没有抑菌作用,乙酸乙酯萃取物、正丁醇萃取物及剩余的水部分均有不同程度的抑菌作用。70%乙醇广东紫珠提取物通过二氯甲烷萃取除去部分脂溶性杂质,即得到广东紫珠抑菌部位,可以此为基础寻找广东紫珠抑菌活性成分,为后期开发利用提供理论依据。 相似文献
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The antimicrobial activity and chemistry of the African Combretaceae has been well studied in recent years. The present study aimed to investigate the phytochemistry and antimicrobial activity of lesser known members of this family viz. C. hereroense, C. apiculatum and C. collinum. Pulverized leaves of C. collinum and C. apiculatum, and the fruit of C. hereroense were extracted with organic solvents and subjected to preparative chromatography. Seventeen phenolic constituents including four phenanthrenes from the fruit of C. hereroense and two known bibenzyls (including a combretastatin) from the leaves of C. collinum were isolated. The compounds were then subsequently tested for their antimicrobial activity against Candida albicans, Mycobacterium fortuitum, Staphylococcus aureus, Escherichia coli and Proteus vulgaris. Pinocembrin showed excellent activity against C. albicans (MIC - 6.25 μg/ml), superior to that of the positive control, fluconazole and against S. aureus (MIC - 12.5 mg/ml). The phenanthrenes (compounds 1, 2, 3 and 5) showed some activity against M. fortuitum and S. aureus with a uniform MIC of 25 μg/ml. From this study it was evident that most stilbenoids and flavonoids from the selected Combretaceae have little or no antimicrobial activity. 相似文献