Herbicide-resistant barnyardgrass (Echinochloa crus-galli) in global rice production

Barnyardgrass (Echinochloa crus-galli (L.) Beauv.), an annual species of the family Poaceae, is a major weed problem in rice-producing countries throughout the globe. Synthetic herbicides can effectively control this grass in rice paddies, but the development of resistant biotypes after the continuous use of the same active ingredients has led to low herbicide efficacy and yield losses. In this review, a summary of resistant-barnyardgrass cases in global rice production is reported based on data from the International Herbicide-Resistant Weed Database. The first case of resistant barnyardgrass in rice paddies was to the photosystem-II inhibitor propanil in the late 1980s. Eighty-five (85) out of 116 cases in the period from 1986 to 2022 refer to resistant barnyardgrass (E. crus-galli var. crus-galli, E. crus-galli var. formosensis and E. crus-galli var. zelayensis) in 16 countries. Barnyardgrass has been found resistant to acetolactate synthase (ALS) inhibitors (34 cases), acetyl-CoA carboxylase (ACCase) inhibitors (23 cases), photosystem-II inhibitors (11 cases), auxin mimics/cellulose biosynthesis inhibitors (9 cases), very long chain fatty acid inhibitors (6 cases), and microtubule assembly inhibitors (1 case). The majority of all resistance cases reported to the active ingredients penoxsulam, bispyribac-sodium, and imazamox (ALS inhibitors), cyhalofop-butyl and fenoxaprop-ethyl (ACCase inhibitors), propanil (photosystem-II inhibitors), and quinclorac (auxin mimics/cellulose biosynthesis inhibitors). Although target-site resistance with specific mutations has been identified, non-target site resistance mainly through herbicide detoxification is also of great concern increasing the chance of multiple herbicide resistance evolution. Rotation of herbicides should be adopted concerning the modes of action used as well as the application methods to mitigate resistance evolution of this weed in rice paddies.     

Cross‐resistance of Echinochloa species to acetolactate synthase inhibitor herbicides

This study was conducted to evaluate the cross‐resistance of acetolactate synthase (ALS) inhibitors with different chemistries, specifically azimsulfuron (sulfonylurea), penoxsulam (triazolopyrimidine sulfonanilide) and bispyribac‐sodium (pyrimidinyl thio benzoate), in Echinochloa oryzicola and Echinochloa crus‐galli that had been collected in South Korea and to investigate their herbicide resistance mechanism. Both Echinochloa spp. showed cross‐resistance to the ALS inhibitors belonging to the above three different chemistries. In a whole plant assay with herbicides alone, the resistant/susceptible ratios for azimsulfuron, penoxsulam and bispyribac‐sodium were 12.6, 28.1 and 1.9 in E. oryzicola and 21.1, 13.7 and 1.8 in E. crus‐galli, respectively. An in vitro ALS enzyme assay with herbicides showed that the I ₅₀‐values of the resistant accessions were approximately two‐to‐three times higher than the susceptible accessions, with no statistical difference, suggesting that the difference in ALS sensitivity cannot explain ALS inhibitor resistance in Echinochloa spp. for azimsulfuron, penoxsulam and bispyribac‐sodium. A whole plant assay with fenitrothion showed that the GR ₅₀‐values significantly decreased in both the resistant E. oryzicola and E. crus‐galli accessions when azimsulfuron, penoxsulam and bispyribac‐sodium were applied with the P450 inhibitor, while no significant decrease was observed in the susceptible accessions when the P450 inhibitor was used. Thus, these results suggest that ALS inhibitor cross‐resistance for azimsulfuron, penoxsulam and bispyribac‐sodium is related to enhanced herbicide metabolism.     

稗草生防菌新月弯孢菌Culvularia lunata菌株J15(2)的安全性和致病力

为了确定生防菌新月弯孢菌Culvularia lunata菌株J15(2)的安全性及防除稗草的潜力,研究了该菌株的寄主范围,接种孢子时稗草叶龄、接种后保湿时间、接种浓度对该菌株致病力的影响,土壤带菌对稗草出苗的影响,以及与化学除草剂的相互作用.结果显示,菌株J15(2)只能在稗草上侵染并扩展;接种孢子后保湿24 h,对2叶期稗草抑制率可达55.6%,对1.5叶期稗草抑制率可达100%;保&48h,对2叶期稗草防效提高至91.6%;接种孢子量需达1012孢子/hm2才能较好地抑制稗草生长;土壤带菌亦可抑制稗草生长.该菌与化学除草剂精噁唑禾草灵或二氯喹啉酸混用能显著提高除稗效果,菌株J15(2) 3.3 × 1012孢子//hm2分别与精噁唑禾草灵有效剂量3.83g/hm2二氯喹啉酸有效剂量250.00g/hm2混用,对稗草防效分别为99.5%和88.6%,远高于这两个除草剂单用时的药效.该试验结果表明,菌株J15(2)具有作为微生物除草剂的开发潜力.     

Genetic and morphologic diversity of <Emphasis Type="Italic">Echinochloa crus-galli</Emphasis> populations from different origins

Echinochloa crus-galli (L.) P. Beauv. (barnyardgrass) is an annual weed that is native to Asia and found throughout the world. The broad ecological tolerance and competitive ability of E. crus-galli makes it the most important weed species in rice. Genetic studies of plants are becoming increasingly common because reliable information is necessary to better understand population dynamics, occurrence of herbicide resistance, and demographic data. Echinochloa crus-galli populations from 34 different locations in Turkey were compared with respect to morphological differences and genetic variation. For morphological variation, five seeds of each population were sown in pots and grown in a screenhouse using a randomized block design. Morphological parameters such as germination speed, flowering time, leaf area, plant height, spikelet length, above-ground biomass, root dry weight and number of seeds were measured. Distinct differences among populations with respect to hierarchical cluster analysis were observed. Genetic variations among populations were performed using random amplified polymorphic DNA (RAPD) markers. The seven RAPD primers amplified 55 bands whose molecular weight varied between 200 and 4000 bp. The percentage of polymorphic bands was 74.54%. Results showed high morphological and genetic variability among individual genotypes within geographic locations. Phenotypic and genetic variability among E. crus-galli populations would be influenced by agricultural practices, crop characteristics, geographic location and herbicide pressure. Differences between weed populations may affect response to chemical or biological control.     

Diclofop-methyl antagonism by broadleaf weed herbicides: the importance of leaf expansion rate

Avena saliva cv. Amuri and A. fatua were sprayed with diclofop methyl (1.0 kg a.i. ha^?1) alone and in combination with 2,4-D (1.1 kg a.i. ha^?1), bentazone (1.0 kg a.i. ha^?1), chlorsulfuron (15 g a.i. ha^?1) or dicamba (0.3 kg a.i. ha^?1). Effects of the herbicides on leaf extension rate during the first 8 to 10 days after spraying and subsequent growth (dry weight) after 57–75 days were determined by comparison with unsprayed plants. Diclofop-methyl applied alone did not cause a decrease in leaf extension rate of A. saliva or A. fatua until at least 4 days after spraying. All broadleaf weed herbicides in combination with diclofop-methyl caused a decrease in leaf extension rate of both species within 2 days of spraying. Ten days after spraying, leaf extension rates for plants sprayed with a broadleaf weed herbicide plus diclofopmethyl (all combinations) were lower than for unsprayed plants but greater than for plants sprayed with diclofop-methyl alone. With the exception of A. fatua sprayed with bentazone, long-term growth of plants sprayed with a broadleaf weed herbicide plus diclofop-methyl (all combinations) was lower than for unsprayed plants but greater than for plants sprayed with diclofop-methyl alone. Bentazone applied with diclofop-methyl caused a substantial decrease in leaf extension rate of A. fatua within 24 h of spraying but at harvest, dry weight of plants from this treatment was similar to or less than that for plants sprayed with diclofop-methyl alone. Application of diclofop-methyl with bentazone at a rate of 0.3 kg a.i. ha^?1 also caused a reduction in leaf extension rate of A. fatua within one day of spraying. At this rate of bentazone, dry weight of plants at harvest was intermediate to that of unsprayed plants and those sprayed with diclofop-methyl alone. It is proposed that decreased leaf expansion rate during the first few days afte spraying is the cause of broadleaf weed herbicide antagonism of diclofop-methyl.     

Joint action of Cochliobolus lunatus and atrazine on Echinochloa crus-galli (L.) Beauv.

The potential of the endemic fungus Cochliobolus lunatus as a biological control agent against Echinochloa crus-galli was investigated. Under appropriate conditions the fungus produced leaf necrosis on E. crus-galli resulting in death of young seedlings. However, plants with more than two leaves mostly recovered after some time. Bean, barley, maize, oat, rye, tomato and wheat were highly resistant to the fungus. In experiments in a climate room or in a glasshouse E. crus-galli with more than two leaves could effectively be controlled by C. lunatus in combination with a sub-lethal dosage of atrazine as low as 2.5 mg m^?2 due to a positive interaction between fungus and herbicide.     

A simulation of herbicide use based on weed spatial distribution

Intensive field surveys were conducted to deter mine the spatial distribution of composite broad leaf and grass weed seedlings in seven maize and five soybean fields in eastern Nebraska in 1992. Farmer fields where herbicides were applied in a 38-cm band over 76-cm spaced rows were chosen for this study. Weed populations were measured in the interrow and in the band-treated intrarow. Spatial maps constructed for grass or broadleaf weeds revealed that individual species as well as species assemblages are highly aggregated. On average, 30% of the sample area in the 12 fields surveyed was free of broadleaf weeds and 70% free of grass weeds in the interrow area (no her bicide). Where a pre-emergence herbicide was applied (intrarow), 71% of the sample area was free of broadleaf weeds and 94% free of grass weeds. Increasing the threshold to some value other than zero resulted in a larger field area not requiring a herbicide application. The results of these distribution studies indicate that herbicide use could be substantially reduced if weed dis tribution maps or real-time plant sensing were available to provide information for intermittent herbicide application systems or refinement of economic thresholds.     

Modelling the effects of herbicide dose and weed density on rice‐weed competition

The effects of herbicide dose on rice‐weed competition were investigated to develop a combined model, which can be utilised to estimate an optimum herbicide dose for a given weed density in paddy rice cultivation. Field studies were conducted in Suwon for rice‐Echinochloa crus‐galli competition and Iksan for rice‐Eleocharis kuroguwai during 2007. The competitive effect of the weeds E. crus‐galli and E. kuroguwai decreased with increasing doses of flucetosulfuron and azimsulfuron, respectively, in the same manner as the standard dose–response curve. The combination of the rectangular hyperbolic model and the standard dose–response curve adequately described the complex effects of herbicide dose and weed competition on rice yield. Parameter estimates were used with the model to predict rice yield and estimate the doses of flucetosulfuron and azimsulfuron required to restrict rice yield loss caused by E. crus‐galli and E. kuroguwai, respectively, to an acceptable level. For a rice yield of 5.0 t ha^?1, the model recommended flucetosulfuron doses of 8.7, 13.4 and 20.1 g a.i. ha^?1 when infested with E. crus‐galli at 12, 24 and 48 plants m^?2 respectively. For a rice yield of 5.2 t ha^?1, the model recommended azimsulfuron doses of 3.9, 7.5 and 12.6 g a.i. ha^?1 when infested with E. kuroguwai at 24, 48 and 96 plants m^?2 respectively. The theoretical outputs of the combined model appear robust and indicate there are opportunities for reduced herbicide use in the field. These now require evaluation under field conditions.     

Spontaneous ultraweak photon emission from rice (Oryza sativa L.) and paddy weeds treated with a sulfonylurea herbicide

All living organisms spontaneously generate ultraweak photon emissions, which originate from biochemical reactions in cells. Current research uses the ultraweak photon emissions from organisms as a novel tool to investigate the physiological states of plants. In this study, we found ultraweak photon emissions from leaf segments of rice and several paddy weed species treated with a sulfonylurea herbicide. There is a definite difference in photon emissions among plant species, and rice (Oryza sativa), barnyardgrass (Echinochloa crus-galli) and Cyperus serotinus showed extremely strong enhancement of photon emissions. Photon emissions from these three species treated with sulfonylurea herbicide were suppressed when the leaf segments were treated with the cytochrome P450 monooxygenase (P450) inhibitors, piperonyl butoxide and malathion. These results suggest that P450 inhibitors affect the ultraweak photon emissions from plants.     

Correlation between the soil seed bank and weed populations in maize fields

Annual weed populations establish every year from persistent seed banks in the soil. This 3 year study investigated the relationship between the number of weed seeds in the soil seed bank and the resultant populations of major broadleaf and grass weeds in 30 maize fields. After planting the crop, 1 m² areas were protected from the pre-emergence herbicide application. Soil samples were collected soon after spraying to a depth of 100 mm and the weed seeds therein were enumerated. The emerged weed seedlings in the field sampling areas were counted over the following 8 weeks. Up to 67 broadleaf species and five grass weeds were identified, although not all were found at every site and some were specific to a region or soil type. For the most abundant weeds in the field plots, on average 2.1–8.2% of the seeds of the broadleaf species and 6.2–11.9% of the seeds of the grass weeds in the soil seed bank emerged in any one year, depending on the species. Overall, the results showed a strong linear relationship between the seed numbers in the soil and the seedling numbers in the field for all the grasses and for most broadleaf weeds. For some species, like Trifolium repens , only a weak relationship was observed. In the case of Chenopodium album , which had the largest seed bank, there was evidence of asymptotic behavior, with seedling emergence leveling off at high seed numbers. An estimate of the soil seed bank combined with knowledge of the germination and behavior of specific weed species would thus have good potential for predicting future weed infestations in maize fields.     

A simple model of crop loss by weed competition from early observations on relative leaf area of the weeds

A new simple empirical model for early prediction of crop losses by weed competition was introduced. This model relates yield loss to relative leaf area of the weeds shortly after crop emergence using the relative damage coefficient q as the single model parameter. The model is derived from the hyperbolic yield density relationship and therefore accounts for the effects of weed density. It is shown that the model also accounts for the effect of different relative times of weed emergence. A strong advantage of the approach is that it can be used when weeds emerge in separate flushes. The regression model described experimental data on sugar-beet – lambsquarters (Beta vulgaris L. –Chenopodium album L.) and maize-barnyard grass (Zea mays L. –Echinochloa crus-galli L.) competition precisely. The model describes a single relationship between crop yield loss and relative leaf area of the weeds over a wide range of weed densities and relative times of weed emergence. Possibilities for scientific and practical application of the model are discussed.     

尖角突脐孢对稻田稗草的防除效果

在温室内进行了露水期、接种量、喷液量及稗草叶龄对尖角突脐孢菌(Exserohilummonoceras)除稗效果的试验.结果表明,适合条件下,尖角突脐孢对4叶期以下的稗草幼苗具有极好的防效.要达到理想的除稗效果需要至少24h的露水期,接种菌液的孢子浓度在1.25×105孢子/ml以上.接种孢子浓度和喷液量对尖角突脐孢除稗效果的影响也极明显,它们与防效呈S曲线关系.     

Spatial and temporal changes in acetolactate synthase activity as affected by new herbicide ZJ0273 in rapeseed, barley and water chickweed

Propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino)benzoate (ZJ0273), derived from a precursor compound pesticide (a derivative of 2-pyrimidinyloxy-N-aryl benzoate), is a novel herbicide used in the rapeseed field. This paper studied the differential tolerance or susceptibility of four plant species viz. Brassica napus L. (rapeseed), Hordeum vulgare L. (barley), Malachium aquaticum (L.) Fries (water chickweed), and Alopecurus aequalis Sobol. (equal alopecurus), to various concentrations of ZJ0273, based on the rate of inhibition of acetolactate synthase (ALS) activity. Brassica napus was observed as the most tolerant among four species in which ALS activity in vivo was hardly affected by ZJ0273 at 100 mg/L (recommended dose in the rapeseed field). In contrast, H. vulgare was found to be more susceptible which was reflected in the relative decline of ALS activity and lower biomass production even under at a lower rate of 10 mg/L of ZJ0273. The two weed species exhibited moderate susceptibility as compared to H. vulgare. The present study confirmed that ZJ0273 at 100 mg/L is the optimal dose of herbicide for rapeseed field. Seedling stage was critical in determining the tolerance of crops and susceptibility of weeds which indicated that over seven leaf stage was much safer for rapeseed whereas the two leaf stage juvenile water chickweed was more susceptible to the same rate of ZJ0273. Spatial and temporal variations in ALS activity as affected by 100 mg/L ZJ0273 in water chickweed, rapeseed and barley also suggested that the synchronized sampling based on the tissue position and seedling stage was necessary to examine precisely, the special difference in tolerance or susceptibility to ALS-inhibiting herbicide. The uppermost younger leaf seemed to be the most appropriate sample for evaluation. Furthermore, the variant tendency of ALS activity from different parts of plants between monocotyledons and dicotyledons to ZJ0273 treatments showed differential response which revealed some possible mechanism related to the altered absorption and translocation of the herbicide.     

Development of herbicide resistance in weeds in a crop rotation with acetolactate synthase‐tolerant sugar beets under varying selection pressure

The development of acetolactate synthase (ALS) tolerant sugar beet provides new opportunities for weed control in sugar beet cultivation. The system consists of an ALS?inhibiting herbicide (foramsulfuron + thiencarbazone‐methyl) and a herbicide‐tolerant sugar beet variety. Previously, the use of ALS‐inhibitors in sugar beet was limited due to the susceptibility of the crop to active ingredients from this mode of action. The postulated benefits of cultivation of the ALS‐tolerant sugar beet are associated with potential risks. Up to now, with no relevant proportion of herbicide‐tolerant crops in Germany, ALS‐inhibitors are used in many different crops. An additional use in sugar beet cultivation could increase the selection pressure for ALS‐resistant weeds. To evaluate the impact of varying intensity of ALS‐inhibitor use on two weed species (Alopecurus myosuroides and Tripleurospermum perforatum) in a crop rotation, field trials were conducted in Germany in two locations from 2014 to 2017. Weed densities, genetic resistance background and crop yields were annually assessed. The results indicate that it is possible to control ALS‐resistant weeds with an adapted herbicide strategy in a crop rotation including herbicide‐tolerant sugar beet. According to the weed density and species, the herbicide strategy must be extended to graminicide treatment in sugar beet, and a residual herbicide must be used in winter wheat. The spread of resistant biotypes in our experiments could not be attributed to the integration of herbicide‐tolerant cultivars, although the application of ALS‐inhibitors promoted the development of resistant weed populations. Annual use of ALS‐inhibitors resulted in significant high weed densities and caused seriously yield losses. Genetic analysis of surviving weed plants confirmed the selection of ALS‐resistant biotypes.     

植物光合作用测定法确定灭草松和特丁津混用的最低致死剂量

在荷兰瓦赫宁根国际植物研究所温室内,用植物光合作用测定法研究了光合作用抑制型除草剂灭草松和特丁津桶混防除6种常见阔叶杂草的最低致死剂量。结果表明,药剂对杂草的生长有明显的抑制作用,在施药2 d后可观察到明显的灭草松的防效,在施药8 d后可观察到明显的特丁津的防效。随着施药剂量的增加,杂草叶片的植物光合作用测定(PPM)值和杂草地上部分鲜重显著下降。供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17 g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20 g/hm2;而番茄不敏感。比较施药后2、4和6 d时杂草叶片的PPM值与施药后16 d时杂草地上部分鲜重防效的相关性可以发现,施药后4 d杂草叶片的PPM值与鲜重防效的相关性最好,相应的R2为0.924 7。表明PPM值可以较好地预测防效,在本试验条件下,施药后4 d的PPM值的预测效果最好。     

Effects of rye cover crop residue and herbicides on weed control in narrow and wide row soybean planting systems

Α three‐year, non‐irrigated field study was conducted in 1998, 1999, and 2000 at the Southern Weed Science Research Unit farm, Stoneville, MS to study the effects of rye cover crop residue, soybean planting systems, and herbicide application programs on the control, density and biomass of several weed species and soybean yield. The soybean planting systems comprised 19 cm rows with high plant density, 57 cm rows with medium plant density, and 95 cm rows with low plant density. The herbicide programs evaluated were pre‐emergence, postemergence, pre‐emergence followed by postemergence, and no herbicide. Flumetsulam and metolachlor were applied pre‐emergence, and acifluorfen, bentazon, and clethodim were applied postemergence. The presence or absence of rye cover crop residue and a soybean planting system did not affect weed control of the species evaluated (browntop millet, barnyard grass, broadleaf signal grass, pitted morningglory, yellow nutsedge, Palmer amaranth and hyssop spurge), when herbicides were applied, regardless of the application program. In addition, rye cover crop residue was not an effective weed management tool when no herbicide was applied, because density and biomass of most weeds evaluated were higher than a no cover crop residue system. Among soybean planting systems, narrow with high plant density soybeans reduced density of grasses, broadleaf weeds and yellow nutsedge by 24–83% and total weed biomass by 38%, compared to wide with low plant density soybeans. Although weed pressure was reduced by narrow with high plant density soybeans, herbicide applications had the most impact on weed control, weed density and biomass. All herbicide programs controlled all weed species 81–100% at two weeks after postemergence herbicide applications, in comparison to no‐herbicide. Density of grasses and all broadleaf weeds as well as total weed biomass was lower with the pre‐emergence followed by postemergence program than these programs alone. Soybean yields were higher in the pre‐emergence followed by postemergence, and postemergence only programs than the pre‐emergence alone program. Planting crops in narrow rows is one cultural method of reducing weed pressure. However, even with the use of this cultural practice, prevalent weed pressure often requires management with herbicides.     

Effect of initial herbicide treatment and planting material on woody weed development and the growth of Eucalyptus nitens and Eucalyptus regnans

A research study of establishment techniques in plantations of Eucalyptus nitens (Deane et Maiden) Maiden and Eucalyptus regnans F Mueller was assessed for growth at age 12 years. Biomass of trees and woody weeds was determined in the 13th year to evaluate the long-term effect of herbicide treatment on plots established with optimum seedling tree and site preparation. Eucalyptus regnans produced only 60% of the biomass of E. nitens. Under-storey woody weed biomass under E. regnans comprised 18–30% of the total biomass compared with 6% for E. nitens plots. There was less total under-storey weed biomass under the herbicide-treated plots, but a similar weed leaf biomass. Under-storey weed leaf biomass contributed 14–40% of the total leaf biomass on the plots. Despite under-storey weed biomass being substantially greater on the no-herbicide plots sampled, this competition had not generally reduced growth of the trees compared with the herbicide-treated plots. Suitable site preparation, providing weed-free conditions at the time of planting, resulted in woody weed control adequate to establish seedlings and allow full growth without the use of herbicides, given suitable seedling material. Although pre-planting herbicide treatment was not necessary for optimum growth of E. nitens grown from bare-root transplants, it did improve growth of slower-growing planting stock.     

Real-time weed detection, decision making and patch spraying in maize, sugarbeet, winter wheat and winter barley

Information on temporal and spatial variation in weed seedling populations within agricultural fields is very important for weed population assessment and management. Most of all, it allows a potential reduction in herbicide use, when post‐emergence herbicides are only applied to field sections with weed infestation levels higher than the economic weed threshold; a review of such work is provided. This paper presents a system for site‐specific weed control in sugarbeet (Beta vulgaris L.), maize (Zea mays L.), winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.), including online weed detection using digital image analysis, computer‐based decision making and global positioning systems (GPS)‐controlled patch spraying. In a 4‐year study, herbicide use with this map‐based approach was reduced in winter cereals by 60% for herbicides against broad‐leaved weeds and 90% for grass weed herbicides. In sugarbeet and maize, average savings for grass weed herbicides were 78% in maize and 36% in sugarbeet. For herbicides against broad‐leaved weeds, 11% were saved in maize and 41% in sugarbeet.     

Differential effect of diclofop-methyl on fatty acid biosynthesis in leaves of sensitive and tolerant plant species

The herbicide diclofop-methyl caused an early and pronounced inhibition of the incorporation of [¹⁴C]acetate into leaf lipids of the sensitive plant species maize (Zea may L.), wild oat (Avena fatua L.), and barnyardgrass (Echinochloa crus-galli L.). With an EC₅₀ value of approximately 10^?7M inhibition was already apparent 0.5–4 hr after herbicide application. The fatty acid biosynthesis of tolerant bean (Phaseolus vulgaris L.), sugar beet (Beta vulgaris L.), and soybean (Glycine max L.) was not affected, with one exception [wheat (Triticum aestivum L.) belongs to the more tolerant species]; the inhibition of fatty acid biosynthesis, however, was in the same order of magnitude as in sensitive plants. More detailed studies showed that in wheat a recovery from inhibition of fatty acid biosynthesis occurred. Four days after herbicide application (0.18 kg diclofop-methyl/ha) in wheat normal fatty acid biosynthesis was restored, whereas in sensitive maize a 60% inhibition was maintained over the whole experimental period (8 days). The results support the view that tolerance of wheat to diclofop-methyl is based on its inactivation in leaves, whereas the tolerance of dicotyledonous species may probably lie at the level of the site of action of diclofop-methyl. In experiments with intact leaves, the inhibition of fatty acid biosynthesis resulted in an enhanced flow of [¹⁴C]acetate into organic acids and amino acids. This effect, however, was not always reproducible in experiments with leaf pieces or isolated root tips.     

棉田除草活性化合物SIOC0426生物活性初步评价

SIOC0426 属2-嘧啶氧基-N-芳基苄胺类选择性除草活性化合物。室内生物活性测定结果表明,SIOC0426具有良好的芽后早期除草活性,对棉花安全,在棉花与重要杂草马唐和反枝苋之间的选择性系数分别为 3.534 和4.299,能有效防除棉花田禾本科杂草和阔叶杂草,对莎草科杂草也有一定抑制作用,使用适期为杂草1~2叶期。田间试验表明,在有效成分90~120 g/hm2剂量范围内,对棉田主要杂草的总防效在73.1% ~87.6%之间。