潍坊地区宠物犬肿瘤临床病理学诊断与发病分析

目的:根据潍坊市宠物犬肿瘤临床病理学诊断病例,对其发病原因进行分析。方法:将我市自2019年4月至2020年4月在潍坊地区多家宠物医院及其诊所收治的70例肿瘤犬病例进行回顾性分析,经组织病理学结果,分析犬肿瘤发病部位和发病性质以及肿瘤犬品种和发病率间的关系。结果:犬肿瘤发生率最高的部位是乳腺,占比为32.86%,其次是皮肤及肛周(27.14%)。不同品种宠物犬的患肿瘤概率不同,其中贵宾犬的患病率最高(21.43%),其次是中华田园犬(20.00%)。结论:经潍坊地区的宠物犬肿瘤病理学诊断与发病分析为宠物犬肿瘤的诊断提供依据,为犬肿瘤的治疗奠定基础。     

44例宠物犬肿瘤病理诊断及分析

收集北京地区2015~2016年宠物医院的部分宠物犬肿瘤病例56例,采用组织病理学方法进行病理诊断,并对患病动物的品种、发生部位、年龄和性别与肿瘤发生关系进行了统计分析。结果发现,56个病例中有44例确诊为肿瘤,其中良性肿瘤21例,包括肛周腺瘤、皮脂腺瘤和肥大细胞瘤等;恶性肿瘤23例,包括乳腺癌、鳞状上皮细胞癌和肛周腺癌等。本次采集的病例中,大型犬与小型犬发病率持平,皮肤、肛周与乳腺部位肿瘤的发生率较高;7岁以后是肿瘤高发期;雌性犬皮肤和乳腺肿瘤的患病率高于雄性,雄性宠物犬肛周腺肿瘤的发生率高于雌性,肿瘤总患病率雄性与雌性持平。本研究为今后宠物犬肿瘤的流行病学及诊断提供了新的参考资料。     

38例犬肿瘤的病理诊断及分析

收集深圳市2010年－2012年部分宠物医院的犬肿瘤病例41例,采用组织病理学方法对犬肿瘤病例进行组织学分类,并对患病动物的年龄、性别和发生部位进行了统计分析。38例确诊肿瘤病例中,皮肤肿瘤有19例,包括鳞状细胞癌4例、基底细胞癌5例、乳头状瘤3例,皮脂腺瘤和肛周腺癌各2例,脂肪瘤、黑色素瘤、角化棘皮瘤各1例;乳腺肿瘤9例,包括乳腺癌3例、乳腺鳞状细胞癌2例、乳腺癌肉瘤2例、黏液癌1例,患犬以雌性为主;其他部位肿瘤分别有纤维肉瘤2例,血管瘤、肺癌、淋巴瘤、睾丸精原细胞瘤、睾丸支柱细胞瘤、组织细胞癌、鳞状细胞癌、乳头状瘤各1例。上述结果显示,犬肿瘤的高发部位是皮肤,其次是乳腺,其发生年龄以7岁以上为多,在发病动物的品种上没有明显差别;除乳腺肿瘤外,其余肿瘤的性别差异不大。本研究为犬肿瘤的流行病学及诊断提供了参考依据。     

犬常见肿瘤流行病学调查及组织病理学诊断

为探讨犬常见肿瘤的发病规律,试验收集哈尔滨地区2016-2017年宠物医院部分犬肿瘤病例71例,采用组织病理学方法对犬肿瘤进行病理学诊断,并对患病动物的品种、性别、年龄、发生部位和饮食情况与肿瘤发生关系进行统计分析。结果显示,71例确诊肿瘤病例中,恶性肿瘤28例,包括鳞状上皮细胞癌3例,基底细胞癌5例,乳腺癌14例,淋巴瘤2例,肾母细胞瘤、精原细胞癌、生殖细胞癌、侵袭性血管黏液瘤各1例;良性肿瘤43例,包括良性乳腺肿瘤15例,纤维瘤10例,平滑肌瘤5例,乳头状瘤和精原细胞瘤各3例,造釉细胞瘤、耵聍腺瘤、脂肪瘤、结节样增生、性索间质细胞瘤、颗粒细胞瘤、肛周腺瘤各1例。上述病例中,乳腺和皮肤是犬肿瘤的高发部位,分别占全部患犬的40.8%和34.0%;患病犬年龄1~16岁不等,平均发病年龄为9.1岁,7岁以后是肿瘤的高发期,中老年犬仍为主要发病群体,但低龄犬发病数量上升;各品种犬均可发生肿瘤,其中杂种犬发病率最高,其次是贵宾犬和京巴犬,且主要为乳腺肿瘤;饮食习惯主要以剩饭剩菜类和肉拌饭类为主,分别占患犬总数的39%和30%;部分肿瘤类型与犬的性别、是否绝育有关,如乳腺肿瘤多发生于未绝育的老年母犬。本研究结果为犬肿瘤的流行病学及诊断提供了参考依据,对犬肿瘤的预防和诊断有一定的借鉴意义。     

26例犬毛囊肿瘤的组织病理学诊断和免疫组织化学分析

本研究旨在为犬毛囊肿瘤的诊断提供最优的免疫标记物,提高肿瘤诊断的准确性,缩短肿瘤的病理学诊断时间,为犬毛囊肿瘤病的临床精准治疗提供帮助.作者收集了26例临床犬毛囊肿瘤病例,分别用免疫标记物CK5/6、CK8/18、CK19、P63、CD34、CD10、Vimentin对肿瘤样本进行免疫组织化学标记(IHC).26例犬毛...     

流浪犬传染性性病肿瘤病理学诊断与治疗

犬传染性性病肿瘤是常见的能够在犬科类传播的一种生殖道肿瘤疾病,常见于有交配行为的流浪犬,该病潜伏期约为2～6个月。本文通过对救助的1只流浪犬进行临床检查、细胞学染色及病理学检查,最终诊断为犬传染性性病肿瘤,影像学检查未发现转移,与救助人协商后同意化疗,经过6周化疗后病犬痊愈,3个月后回访,未出现复发。     

犬乳腺肿瘤病理学观察与分析

通过利用石蜡切片制作技术、HE染色技术及显微摄影技术,结合临床资料,对犬乳腺肿瘤进行病例分析,以期能够鉴别诊断犬的良性和恶性乳腺肿瘤。将收集到的10个具有典型病理变化乳腺肿瘤组织制作成病理切片,用HE染液进行染色,并利用光学显微镜进行观察。结合临床资料,对犬乳腺肿瘤进行病例分析,并对犬乳腺肿瘤病例的基本信息进行归纳,发现平均患病年龄为8.5岁老犬,然后对术后切除的肿瘤进行了病理基本的鉴别诊断,发现恶性肿瘤6例,并对所制作乳腺肿瘤切片中具有典型病理变化的切片作了细致的观察和分类描述。     

15例宠物犬临床肿瘤的病理组织学诊断与分析

笔者对新疆农业大学动物中心医院2018年7月-10月收集的15例犬临床肿瘤样病例进行病理组织学诊断,并对各类型肿瘤进行分类,以探讨宠物犬临床肿瘤疾病的发病特点。在15例肿瘤病例中,单发性肿瘤14例,多发性病例1例;发生在皮肤的肿瘤9例,发生在乳腺区的肿瘤4例,发生在子宫的肿瘤2例。在新疆农业大学动物中心医院接诊的肿瘤病例当中,以皮肤肿瘤占比最高(56%),其次为乳腺区肿瘤(25%),最后为子宫肿瘤(14%)。雌性发病犬为9只,占比60%;雄性犬发病为6只,占比40%。以上结果为新疆地区的小动物肿瘤流行病学提供了数据,对临床诊断有一定的指导意义。     

犬猫乳腺肿瘤的组织病理学诊断

<正>犬猫乳腺肿瘤是兽医临床上常见的一种肿瘤疾病,约占母犬母猫肿瘤疾病总发病率的50%,公犬公猫则比较少见。据国外文献记载,犬的乳腺肿瘤是仅次于皮肤肿瘤的最常见肿瘤,约50%为恶性;猫的乳腺是仅次于肝脏和皮肤的最易发生肿瘤的组织,     

犬睑板腺瘤组织病理学诊断病例

犬睑板腺瘤(Meibomian Gland Adenoma)是一种变异的皮脂腺瘤,属于眼部皮肤的良性肿瘤,是犬常见肿瘤之一,猫几乎不发生该肿瘤[1].本实验室于2010年接收了一例犬的眼睑肿物病例,经组织病理学诊断为犬睑板腺瘤,报告如下. 1临床资料 萨摩耶犬,雄性,6岁.下服睑肿瘤,绿豆粒大小,近半年逐渐增大.     

Correlation between the histopathological diagnosis by AgNOR count and AgNOR area in canine mammary tumors

Background: Mammary tumors are the most common type of tumor in female dogs. The histopathological diagnosis is usually made by a hematoxylin-eosin (HE) staining of the tumor, which then requires a pathologist's judgment for assessment of malignancy. The purpose of this study was to investigate an alternative silver staining of some argyrophilic nucleolar organizer regions (AgNOR) for improving the diagnostic accuracy with mammary tumors.
Hypothesis: There is a correlation between the histopathological diagnosis by AgNOR count and AgNOR area in canine mammary tumors.
Animals: Seventy-three canine mammary tumors from 33 female dogs.
Materials and Methods: The AgNOR staining was evaluated retrospectively in 73 canine mammary tumors with a parallel HE staining as a "Gold Standard." Both a quantitative manual counting method and a qualitative computerized morphometric method were tested.
Result: The result from both methods indicated a clinically relevant difference in the mean values of the AgNOR in the following 4 categories: malignant, benign, hyperplastic, and normal mammary tissue. The counting method was superior, with 89% of the cases given a correct diagnosis of a malignant or a nonmalignant canine mammary tumor. The 2 methods were then compared to test their ability to classify the tumors correctly. Again, the counting method was the most reliable method, with a sensitivity of 80% and a specificity of 76% when the upper 50% of the AgNOR counts were presumed malignant.
Conclusion and Clinical Importance: The results indicated that an AgNOR test could be an aid to pathologists as a prognostic indicator or to assist them in deciding between a benign or a malignant diagnosis in questionable cases.     

南京市部分地区暴发流行山羊痘的诊断与防制

1999年 6月～ 10月在我市的江浦县、溧水县、高淳县养羊较集中的部分乡 (镇 )相继暴发流行一种疫病 ,其临床表现为体温升高 ,眼、鼻流粘液 ,唇、鼻、乳房等少毛部位有大量红色丘疹 ,并有破溃、结痂等特征 ,发病率和病死率均较高 ,而且传播快 ,给当地养羊农户造成了很大的经济损失。现将此次羊病发生发展情况及诊断与防制工作情况报告如下。1　临床症状病羊初期精神沉郁 ,食欲减少 ,体温上升至 41 5℃ ;皮肤出现大量红色丘疹 ,以眼、唇、鼻、耳背、乳房和大腿内侧比较明显 ;眼睑肿胀 ,流粘脓性分泌物 ;部分病羊呼吸急促、咳嗽 ,鼻腔有浆液性…     

碘伏对观赏犬子宫炎的治疗

子宫内膜炎是观赏犬生殖系统的常见病 ,也是观赏犬繁殖障碍的重要原因。用碘伏治疗 ,具有操作简便、疗效确实、无其他并发症等许多优点。1　临床诊断　从病犬阴门排出灰黄色或红褐色或灰白色脓性分泌物 ,具有臭味。阴门稍肿大、松弛 ,病犬爱用舌舔。体温有时 40℃以上 ,精神不振 ,喜卧 ,频频排尿 ,但每次排尿少。食欲减退或拒食 ,常常转为慢性经过。2　阴道检查　阴道内有不洁分泌物、异臭。子宫颈口充血、肿胀、松弛、开张 ,并可见有分泌物从中流入阴道内。3　腹壁触诊　可感子宫缺乏弹性 ,子宫壁增厚 ,当子宫腔有炎性分泌物蓄积时 ,则有波…     

Common germline haplotypes and genotypes identified in BRCA2 exon 11 of dogs with mammary tumours and histopathological analyses

The canine BRCA2 is a tumor supressor gene which encodes the BRCA2 protein, involved in DNA repair through interaction with the RAD51 recombinase. This process is mediated by eigth BRC repeats that are encoded by BRCA2 exon 11. Two variants corresponding to human mutations in human BRC3 repeat have been reported in canine BRC3 repeat. In addition, other variants have also been described in canine BRCA2 exon 11. Considering the importance of polymorphisms in human BRCA2 to breast cancer development, this study aimed to investigate the frequency of variants in BRCA2 exon 11 in 48 blood and tissue DNA samples from bitches with canine mammary tumors (CMT), as well as, to analyze tumor stage and histopathological features. Seven Single Nucleotide Polymorphisms (SNPs) were identified, three of which were evaluated as possibily or probably deleterious variant. Interestingly, almost all the 22 mammary tumors (except one) which presented a clinical staging equal to or greater than III carried at least one mutant allele of these three variants. Besides that, no statistically significant correlation was observed between any of the reported SNPs in heterozygosis or homozygosis and either dogs data (such as breed, age or disease stage) or mammary tumors histopathological characteristics. A total of 97.9% of bitches had one to three polymorphisms of the seven identified in this study, which suggests a possibly correlation between the canine BRCA2 exon 11 polymorphisms and mammary carcinogenesis.     

The diagnosis and prognosis of synovial tumors in dogs: 35 cases

Although synovial cell sarcoma is reported to be the most common neoplasm of the canine synovium, this retrospective study of 35 canine synovial tumors found that the majority were of histiocytic origin. Five (14.3%) synovial cell sarcomas were identified by positive immunohistochemical staining with antibodies to cytokeratin. Eighteen (51.4%) histiocytic sarcomas were identified by cell morphology and immunohistochemical staining with antibodies to CD18. Six (17.1%) synovial myxomas were identified by histologic pattern. The remaining six (17.1%) synovial tumors represented a variety of sarcomas, including two malignant fibrous histiocytomas (actin positive), one fibrosarcoma, one chondrosarcoma, and two undifferentiated sarcomas. Rottweilers were overrepresented in the histiocytic sarcoma category and Doberman Pinschers were overrepresented in the synovial myxoma category. The average survival time was 31.8 months for dogs with synovial cell sarcoma, 5.3 months for dogs with histiocytic sarcoma, 30.7 months for dogs with synovial myxoma, and 3.5 months for dogs with other sarcomas. Among the dogs with follow-up information available, metastatic disease was detected in 25% of dogs with synovial cell sarcoma, in 91% of dogs with histiocytic sarcoma, in none of the dogs with synovial myxoma, and in 100% of dogs with other sarcomas. Immunohistochemical staining for cytokeratin, CD18, and smooth muscle actin is recommended to make the diagnosis and thereby predict the behavior of synovial tumors in dogs.     

Utility of contrast-enhanced ultrasound in differential diagnosis of adrenal tumors in dogs

This prospective case study aimed to clarify the clinical significance of contrast-enhanced ultrasound (CEUS) for the differential diagnosis of canine adrenal tumors. Forty-three client-owned dogs with adrenal tumors were included. All dogs underwent CEUS, which was evaluated qualitatively and quantitatively. The peak signal intensity (PI), time to peak signal intensity (TPI), mean transit time (MTT), upslope, and downslope were calculated for each time-intensity curve. The histopathological diagnosis of each resected mass was compared with the CEUS findings and parameters. Enhancement distribution, vascularity, tortuous nourishing vessels, enhancement pattern, and late-phase enhancement did not differ significantly between adrenal cortical adenoma (CA), adenocarcinoma (CAC), and pheochromocytoma (PHEO) in qualitative assessment. In PHEO, the TPI was significantly more rapid compared with that in CA (P=0.0287) and CAC (P=0.0404). The MTT in PHEO was significantly shorter than that in CA (P=0.0016) and CAC (P=0.0003). Upslope in PHEO was larger than that in CAC (P=0.0406). Downslope in PHEO was significantly larger than that in CA (P=0.0048) and CAC (P=0.0018). A receiver operating characteristic curve analysis demonstrated that the area under the MTT curve yielded 0.91 for distinguishing PHEO from adrenocortical tumors in dogs; an MTT cut-off value less than 6,225 msec yielded a sensitivity of 69%, specificity of 94%, and likelihood ratio of 12.46. CEUS appears to be clinically applicable for the differential diagnosis between cortical and medullary origins of primary adrenal tumors in dogs.     

Clinical symptoms and diagnosis of encephalitozoonosis in pet rabbits

Infections with Encephalitozoon cuniculi in rabbits are observed at increasing frequency and are known as opportunistic infections in immunocompromised humans. 191 pet rabbits with suspected encephalitozoonosis, presented at the Animal Hospital of the Veterinary University of Vienna (Austria), were included in this study. Rabbits were serologically examined for antibodies against E. cuniculi (144 positive out of 184 rabbits with suspected encephalitozoonosis compared to 14 positive out of 40 clinically healthy rabbits tested as part of a standard health check) and Toxoplasma gondii (8 positive out of 157). Of the 144 seropositive rabbits with clinical signs, 75% showed neurological symptoms, 14.6% demonstrated phacoclastic uveitis and 3.5% suffered from renal failure. 6.9% of the animals had combined symptoms. Vestibular disease dominated within the rabbits that showed neurological symptoms. Polymerase chain reaction (PCR) could not detect parasite DNA in urine or cerebrospinal fluid (CSF), but did so in 4 out of 5 samples of liquefied lens material in cases with phacoclastic uveitis due to lens capsule rupture. Additionally further diagnostic procedures, such as inspection of the external ear canal (N=69), radiography of the tympanic bullae (N=65) were performed to rule out differential diagnosis. 54.2% of the patients exhibiting neurological symptoms recovered within a few days, while 87.5% of the rabbits suffering from renal failure died or had to be euthanized.