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1.
Ferreira SR de Araújo JV Braga FR Araujo JM Frassy LN Ferreira AS 《Veterinary research communications》2011,35(8):553-558
Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided
into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3
did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing
P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h
after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately
100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity
parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration
and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs. 相似文献
2.
Camacho AT Guitian FJ Pallas E Gestal JJ Olmeda AS Habela MA Telford SR Spielman A 《Tropical animal health and production》2005,37(4):293-302
The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B. caballi. Sixty serum samples (control group) were taken from healthy horses pastured on two farms, and examined for evidence of equine T. equi and B. caballi infection by indirect fluorescent antibody test (IFAT). Of the 60 samples, 24 (40%) and 17 (28.3%) samples were positive for T. equi and B. caballi, respectively. Twelve (20%) samples were positive for both parasites. Haematology and serum biochemistry were compared between controls and a series of 36 horses clinically affected by T. equi (25) or B. caballi (11). Compared with the healthy group, there was a 43% and 37% decrease in the haematocrit for T. equi and B. caballi infection, respectively. Parasitaemic horses presented an intense anaemia and serum biochemistry signs of liver damage. The anaemia was more severe in T. equi-infected than in B. caballi-infected horses. Our results suggest that equine piroplasmosis is widespread in the region and is a cause for concern. 相似文献
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Hossein Nourani Somayeh Matin Abass Nouri Hamidreza Azizi 《Tropical animal health and production》2010,42(6):1225-1227
Bovine sarcocystosis is caused by Sarcocystis cruzi and is known to cause considerable morbidity and mortality in cattle. This species is distributed worldwide in cattle and
is the most prevalent of the Sarcocystis species infecting cattle. There is high infection rate of sarcocyst in cattle in Iran, but to our knowledge, there is no
study about identification of Sarcocystis species. This work aimed to survey prevalence of S. cruzi cyst in slaughtered cattle of Isfahan, Iran. In this study, esophageal and diaphragmatic muscles of 100 cattle were collected
from Fesaran abattoir of Isfahan and examined for the presence of Sarcocystis spp. cysts macroscopically and microscopically. No macroscopic sarcocysts were found in any of the samples. In light microscopy,
89 out of 100 cattle (89%) had thin-walled cysts of S. cruzi, while 21 out of them (21%) had thick-walled sarcocysts. In addition to light microscopy, ultrastructural features of the
thin-walled cyst confirmed the presence of S. cruzi. 相似文献
5.
Ozlem Ozmen Mehmet Kale Mehmet Haligur Sibel Yavru 《Tropical animal health and production》2009,41(6):951-958
In this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep
and lambs that suffer from simultaneous naturally occurring BTV, PPRV and SPV outbreaks. SPV was diagnosed macroscopically
and histopathologically, BTV was diagnosed by ELISA, and PPRV was diagnosed pathologically and by ELISA. Clinically fever,
diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia,
cutaneous eruption with erythematous areas especially noticeable in wool-free parts of the body and axilla lesions evolving
into papules were observed. At necropsy, the most effected organs were lungs and gut. Subepicardial hemorrhages were also
commonly seen. While typical pox lesions were observed in some lambs, usually fibrinous pleuropneumonia was more prominent
lung lesion. SPV and PPRV lesions were seen at the histopathological examination of the lesioned tissues, BT lesions were
mild than SPV and PPRV microscopically. Serum and leukocyte samples of 15 animals were examined for PPRV and BTV by ELISA;
5 samples were positive for PPRV and 6 BTV, 4 were positive for both PPRV and BTV simultaneously. One hundred animals died,
most were lambs. Mortality rates were 100% in lambs and 80% in the herd. 相似文献
6.
Cabrera A Morales-Erasto V Salgado-Miranda C Blackall PJ Soriano-Vargas E 《Tropical animal health and production》2011,43(3):549-551
Avibacterium paragallinarum is the causative agent of infectious coryza, an acute respiratory disease of chickens. In this study, a total of 28 isolates
of A. paragallinarum from Ecuador were serotyped by the hemagglutinin scheme which recognizes nine serovars. Out of 28 isolates, 17 isolates belonged
to serovar A-3, and five isolates to each serovars B-1 and C-1, whereas one isolate was non-typeable. This is the first report
of A. paragallinarum serovar A-3 outside Brazil and serovar C-1 outside Japan. 相似文献
7.
Amanda Bonalume Cordeiro de Morais Carmen Alicia Daza Bolaños Ana Carolina Alves Cássia Yumi Ikuta Gustavo Henrique Batista Lara Marcos Bryan Heinemann Rogério Giuffrida Fernando Paganini Listoni Mateus de Souza Ribeiro Mioni Rodrigo Garcia Motta Shinji Takai Márcio Garcia Ribeiro 《Tropical animal health and production》2018,50(6):1319-1326
8.
Hao Wang Zihua Li Fu Gao Jiaqing Zhao Mingxing Zhu Xin He Nan Niu Wei Zhao 《Veterinary research communications》2016,40(2):73-79
Objective
This study aims to investigate the immunoprotection of recombinant Eg.P29 (rEg.P29) vaccine and analyze the underlying mechanism in sheep.Methods
Three groups of male sheep were immunized subcutaneously with rEg.P29 and PBS, Freund’s complete adjuvant as controls, respectively. After prime-boost vaccination, the sheep were challenged with encapsulated Echinococcus granulosus eggs. The percentage of protection in sheep was determined 36 weeks after the infection. Humoral immune response was analyzed for specific IgG, IgG1, IgG2, IgM and IgE levels. Moreover, cytokines including interferon (IFN)-γ, interleukin (IL)-2, IL-4,and IL-10 were also evaluated.Results
Immunization with rEg.P29 induced protective immune responses up to 94.5 %, compared with immunoadjuvant group. The levels of specific IgG, IgG1, IgG2, and IgE as well as IFN-γ, IL-2, and IL-4 significantly increased after two immunizations (P < 0.05); however, the levels of IgM and IL-10 did not show difference.Conclusion
rEg.P29 showed Immunoprotection and induced Th1 and Th2 immune responses; hence, rEg.P29 is a potential vaccine for E. granulosus infection.9.
Thallyta Maria Vieira Leydiana Duarte Fonseca Gabriela Almeida Bastos Viviane de Oliveira Vasconcelos Maria Luíza França Silva Franciellen Morais-Costa Adriano Vinícius de Paiva Ferreira Neide Judith Faria de Oliveira Eduardo Robson Duarte 《Veterinary research communications》2017,41(2):99-106
Objective
This study evaluated the effects in vitro and in vivo of Agaricus blazei against Haemonchus contortus in sheep.Methods
The in vitro efficacy of aqueous extract on egg hatching inhibition (EHI) was investigated and after 72 h incubation with varying concentrations the effects on, blastomeres, embryonated eggs, and first stage larvae (L1) were evaluated. Larval development inhibition (LDI) for dry powder and the aqueous extract were evaluated in fecal cultures of sheep infected with H. contortus. In vivo efficacy was determined by reduction in fecal egg count (FEC). Lambs were treated with powder A. blazei (11.4 g/kg pc) or trichlorfon, or were untreated and the possible toxicity of this fungus was monitored by plasmatic enzyme analysis.Results
Concentrations equal to and higher than 3.62 mg/mL and of aqueous extract were 100% effective in the EHI test. In the LDI test, LC90 was estimated for 5.66 and 106.0 mg/g fecal culture for aqueous extract and powder, respectively. The mean FEC in lambs 14 days post-treatment with A. blazei powder was significantly lower than observed for the negative control, and the serum levels of aspartate transaminase and alanine transaminase were normal.Conclusion
The fungi supplementation promotes, respectively, high and moderate anthelmintic efficacy with in vitro and in vivo tests, respectively, suggesting it as an alternative or complementary treatment for haemonchosis in sheep.10.
Benjamin Obukowho Emikpe Mohammed Y. Sabri Stephen O. Akpavie Mohammed Zamri-Saad 《Veterinary research communications》2010,34(7):569-578
Nigerian strain of Peste des Petit Ruminant (PPR) virus and Mannheimia haemolytica (MH) biotype A serotype 2, was used successfully to reproduce a concurrent disease in West African Dwarf goats. The development
of the various pathological features were studied at regular intervals following infection. The acute inflammatory reaction
which had developed by day 3 after initial infection was characterised by flooding of the alveoli by neutrophils, oedema,
hemorrhage and syncytial cells together with a moderate bronchial and bronchiolar epithelial necrosis. This progressed to
a milder acute broncho interstitial pneumonia with giant cells. At this stage, the mucosal immunity were well developed especially
the aggregate form of NALT and more of nodular forms of BALT. The organisms were demonstrated with strong immunostaining in
the necrotic center, necrotic alveolar wall, fibrin, serous exudate, and degenerated leukocyte in the alveoli and respiratory
airways. The bacterial antigens were observed as a strong immunostaining in the blood vessels of the nasal septum, sinusoid
in the liver and interstium of the kidney, cytoplasm of alveolar macrophages, pneumocytes, bronchial and bronchiolar epithelium,
in the monocytes in the blood vessels. These findings confirmed the enhancement of MH tropism especially in the respiratory
tract, liver and kidney. It also showed that West african dwarf goats are highly susceptible to the intratracheal combined
infection of PPR virus and MH. The fact that the infection induces strong mucosal responses, this phenomenon can be explored
in Africa with the use of combined PPR virus and MH intranasal vaccines to curtail the menace of pneumonia associated with
the combined infection on field. 相似文献
11.
Dilmaghani M Ahmadi M Zahraei Salehi T Talebi A 《Veterinary research communications》2011,35(3):133-143
Salmonella enterica subspecies enterica serovar Typhimurium causes food-borne outbreaks and systemic diseases in humans and animals. groEL gene (also known as mopA gene in S. Typhimurium), possessing conserved sequence, plays an important role in invasion of bacteria. The purpose of present study
was to identify the polymorphism of groEL gene among different avians in different regions by PCR-RFLP method. Fifty two S. Typhimurium isolates (Broiler (n = 13), Layer (n = 12), Duck (n = 5), Goose (n = 5), Sparrow (n = 8), Canary (n = 3), Pigeon (n = 5) and Casco parrot (n = 1). were identified using serotyping as well as multiplex-PCR. Then, amplification of groEL gene performed and amplified products subjected to restriction digestion with BsuRI enzyme. Three RFLP profiles, A, B and
C, generated DNA fragments between approximately 100–1,000 bp in size, were observed. The RFLP profile A was observed in 35
(67.3%), profile B in 14 (26.9%) and profile C in 3 (5.77%) of isolates. S. Typhimurium isolates recovered from 13 broilers (two of which profile A, 9 profile B and 2 profile C) and from 8 sparrows
(two of which profile A, 5 profile B and 1 profile C) showed all three profiles, but 12 layers and other avians (including
Canary (n = 3), Goose (n = 5), Duck (n = 5), Pigeon (n = 5) and Casco parrot (n = 1)) showed profile A. None of these profiles was allotted for a special region. The result of present study showed that
S. Typhimurium undergoes genetic mutations in groEL gene under unpleasant milieu in different regions and in different avians. Thus, genetic diversity, despite conserved nature
of groEL gene in S. Typhimurium, may exist but it depends on the condition where bacteria have settled. To our knowledge, three RFLP profiles
of groEL gene generated by BsuRI restriction enzyme were not reported previously. 相似文献
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Hulya Turutoglu Mustafa Hasoksuz Dilek Ozturk Murat Yildirim Sonay Sagnak 《Veterinary research communications》2009,33(8):945-956
Although methicillin-resistant Staphylococcus aureus (MRSA) were generally isolated from human beings; these agents were recently isolated from various animal species. It has
been shown that MRSA isolates are not only resistant to beta-lactam antibiotics, but can also be resistant to the other commonly
used antibiotics. In this study, 18 phenotypic methicillin resistant S. aureus isolates from bovine mastitis cases were analyzed by PCR for the presence of mecA gene encoding methicillin resistance and aac(6′)/aph(2″), aph(3′)-IIIa and ant(4′)-Ia genes encoding aminoglycoside resistance. Out of 18 S. aureus isolates (oxacillin MICs, ≥4 μg/ml), 3 were positive for mecA gene. Only one from 3 mecA positive isolates was positive for genes encoding aminoglycoside-modifying enzymes and this isolate carried aac(6′)/aph(2″) in combination with aph(3′)-IIIa gene. The aph(3′)-IIIa gene was detected in 3 isolates. These three isolates carrying the aminoglycoside-modifying enzyme genes were resistant to
gentamicin, kanamycin and neomycin. The mecA gene of 3 MRSA isolates was sequenced. All three mecA genes of these isolates were identical to that found in human MRSA strains, except a one-base substitution at nucleotide
position 757. From the data presented in this study, it can be concluded that MRSA isolated from bovine mastitis may be originated
from human beings, but further studies are needed to investigate the possibility of zoonotic transfer of MRSA. 相似文献
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Fredrick Kaingu Dandan Liu Lele Wang Jianping Tao Rebecca Waihenya Helen Kutima 《Tropical animal health and production》2017,49(4):823-828
Anticoccidial effects of Aloe secundiflora crude leaf extract was tested in broiler chickens following oral infection with Eimeria tenella. Sixty 22-day-old birds were divided into six groups of ten birds each. Three treatment groups A, B, and C were fed with the extract (100, 250, and 500 mg/day, respectively) mixed in feed for 10 days, and three control groups: group D (drug control) administered 300 mg/l of sulfachloropyrazine sodium soluble powder in drinking water for 5 days, group E (infected/non-medicated positive control), and group F (uninfected/non-medicated negative control). Except for group F, all groups were orally inoculated with 75,000 sporulated oocysts of E. tenella. The effects of the extract on E. tenella infection were evaluated by severity of bloody diarrhea, body weight (BW) gain, oocyst output, and lesion score. No bird in the treated groups died of coccidiosis, and severity of bloody diarrhea was milder than in the positive control group. BW gains in the treated groups were significantly higher than in group E (p < 0.05). The lesion scores of the treated groups were significantly lower than that of group E. Oocyst output in groups A, B, and C were 11.23, 8.24, and 6.82 × 106, respectively. As compared with the negative control group (12.84 × 106), the reductions in oocyst production were 12.54, 35.83, and 46.88%, respectively. Oocyst output significantly reduced with an increase in Aloe dosage. The findings of this study suggest that Aloe secundiflora extract presents an alternative anticoccidial agent for the control of avian coccidiosis. 相似文献
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Liu Q Cai J Zhao Q Shang L Ma R Wang X Li J Hu G Jin H Gao H 《Tropical animal health and production》2011,43(4):741-743
The seroprevalence of Toxoplasma gondii infection in yaks (Bos grunniens) was surveyed in Qinghai Province, northwestern China in May and June 2010. A total of 650 serum samples were collected from
six counties and assayed for T. gondii antibodies by an indirect hemagglutination test. Antibodies to T. gondii were found in 35.08% (228/650) with the highest rate of 55.34% in Chengduo County. The results of the present survey indicated
that infection with T. gondii in cattle is widely spread in China, including yaks in Qinghai Province. 相似文献
19.
Farhan Anwar Khan Zafar Iqbal Chaudhry Muhammad Ijaz Ali Shahid Khan Naima Mumtaz Ijaz Ahmad 《Tropical animal health and production》2010,42(4):633-638
Tissue samples were collected at random from cattle (Bos taurus) and buffalo (Bubalus bubalis) from an abattoir of the district of Lahore and were analyzed for the presence of Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis through acid-fast staining and polymerase chain reaction (PCR). Body condition of animals and diarrhea were recorded. Most
of the animals were emaciated. Diarrhea was noticed in 15.6% of buffaloes and 19.2% of cattle. Intestinal pathology was observed
in 29% of buffaloes and 32.8% of cattle. Number of mesenteric lymph node (MLN) showing gross lesions was a bit higher (35.6%)
in cattle than buffalo (31.2%). Acid-fast staining of tissue scraping smears revealed the presence of acid-fast bacilli (AFB)
in 17.4% intestinal and 16.4% MLN tissue samples in buffalo, while in cattle 19.2% intestinal and 17.8% MLN were found positive
for AFB. In buffaloes, PCR confirmed 12.8% intestinal and 12.4% MLN positive samples for M. avium subsp. paratuberculosis. However, in cattle, PCR analysis demonstrated 14.2% positive results for M. avium subsp. paratuberculosis in both MLN and intestinal tissue samples. PCR also confirmed M. bovis in 5.8% of cattle and 5% of buffalo MLN and intestinal tissues. PCR positive tissue samples for M. avium subsp. paratuberculosis were from those animals which were emaciated, having diarrhea, and severe gross lesions. AFB were also detected in tissue
scraping smears of these animals. It is concluded that infection by various mycobacterium species can be differentiated by
PCR, which is not possible by acid-fast staining technique. 相似文献
20.
Simón F Kramer LH Román A Blasini W Morchón R Marcos-Atxutegi C Grandi G Genchi C 《Veterinary research communications》2007,31(2):161-171
Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts, with infections occasionally being reported in humans. Studies have shown that both dirofilarial
antigens and those derived from its bacterial endosymbiont Wolbachia, interact with the host organism during canine, feline and human infections and participate in the development of the pathology
and in the regulation of the host’s immune response. Both innate and acquired immune responses are observed and the development
of the acquired response may depend on the host and, or on its parasitological status. This review aims at illustrating current
research on the role of both D. immitis and Wolbachia, in the immunology and immunopathology of dirofilariosis. 相似文献