Fate of glyphosate in Agropyron repens (L.) Beauv. growing under low temperature conditions

Absorption, translocation and distribution of ¹⁴C-glyphosate were examined in Agropyron repens (L.) Beauv. plants growing under field conditions in the autumn. Glyphosate absorption did not increase beyond 3 days after application, whereas translocation to the rhizomes continued up to 7 days after application. The translocated glyphosate accumulated more in new rhizomes than in older parts of the rhizomes. Ten per cent of the glyphosate translocated out of the treated shoot was recovered in younger shoots 7 days after application. Plants harvested the following spring contained less than 20% of the glyphosate originally applied. Although a growth cabinet experiment indicated that 34% of the glyphosate in the rhizomes of treated plants could be remobi-lized into new aerial shoots, considerably less was recovered in new, aerial shoots in the spring in the field-grown plants. Freezing experiments showed that glyphosate translocation to the rhizomes was only prevented when cold treatment caused visible damage to A. repens foliage.     

Factors affecting glyphosate activity in Imperata cylindrica (L) Beau, and Cyperus votundus L. I. Effect of soil moisture

Established Greenhouse grown plants of cogongrass Imperata cylindrica (L) Beauv.) and purple nutsedge (Cyperus rotundusL.) were given three different soil moisture regimes; field capacity, moderate stress and extreme stress, followed 6 weeks later by glyphosate [(N-phosphonomeihyl) glydne] applications to the shoots at 0.2,0.4 and 0.8 kg/ha for Imperata and 0.3,0.6 and 1.12 kg/ha for Cyperus. Field capacity watering stimulated most vegetative growth in hoth species. Glyphosate given at field capacity decreased shoot dry weight in both species, and rhizome length, rhizome dry weight and total carbohydrate in Imperata and total number of tuber-bulbs in Cyperus. In contrast. at extreme soil moisture stress, glyphosate showed reduced activity which appeared to be related to the physiological and morphological behaviour of the plants arising from the drought trealment. Application of waier to the roots of the plants grown at soil moisture stress. I week before and I week afler glypbosate spraying, enhanced glyphosate activity, probably because of the recovery of processes disturbed by ibe soil moisture deficit.     

Glyphosate effects on Canada thistle (Cirsium arvense) roots, root buds, and shoots

Glyphosate ? ? Mention of irademark or proprietary product does not constitute a gtiarantee or warranty oC the product by the U.S. Department of Agriculture and does nut imply its approval to the exclusion of other products thai may also be suitable.
was sprayed at 0009–1·12 kg a.i. ha^?1 on the foliage of large potted glasshouse-grown Canada thistle [Cirsium arvense (L.) Scop.], which had extensive, well-developed roots. Increasing the glyphosate rate progressively reduced the total number of visible adventitious root buds plus emerged secondary shoots per plant proportionately more than root biomass, 10 days after treatment. Cortical tissue of thickened propagative roots became soft, water-soaked, darkened, and some regions decomposed, exposing strands of vascular tissue. Lateral roots completely decomposed. When thickened roots were segmented to stimulate secondary shoot emergence from root buds 10 days after foliar treatment, Fewer secondary shoots emerged than expected from the number of visible adventitious root buds present on both control and herbicide-treated plants. Increasing the rate of glyphosate also reduced the regrowth potential of root buds proportionately more than root biomass. Regrowth potential was measured as the number of emerged secondary shoots 35 days after segmenting unearthed roots from plants that had been sprayed 10 days earlier. When foliar-applied at 0·28 kg ha^?1, glyphosate decreased the regrowth potential of root buds to zero in 2 and 3 days, as measured by secondary shoot dry weight and number, respectively, even though root fresh weight was unchanged 3 days after foliar treatment. These dose-response and time-course experiments demonstrate that glyphosate did not reduce root biomass as much as it decreased root bud numbers and secondary shoot regrowth potential from root buds.     

The effectiveness of old and new strategies for the long‐term control of Pteridium aquilinum,an 8‐year test

There is a need for management strategies to control dominant perennial weeds and restore seminatural communities. We compared the effects of five weed control treatments on dense Pteridium aquilinum relative to an untreated experimental control over an 8‐year period with the aim of restoring acid grassland. The weed control treatments tested were as follows: cutting and bruising, both twice and thrice annually, and herbicide treatment (asulam in year 1 followed by annual spot retreatment of all emergent fronds). Pteridium aquilinum performance and plant species composition were monitored. Data were analysed using Bayesian mixed‐effect models and multivariate techniques. Cutting twice and thrice yearly and the asulam treatment all reduced frond density to zero; both bruising treatments were ineffective. The plant communities in the cut and asulam‐treated plots showed differences from the untreated and bruised plots; the asulam‐treated plots contained more ruderal species and the cut plots were more typical of acid grassland. Acid grassland recovery was fastest in the asulam‐treated plots, but the cut plots caught up after approximately 5 years. There were two important conclusions. First, an intractable weed like P. aquilinum can be eradicated and a vegetation more suited for grazing can be achieved by the continuous application of some treatments over many years. Here, success was achieved by cutting twice/thrice annually, or by a single asulam application followed by annual spot spraying of all emergent fronds for 8 years. Second, bruising, a treatment favoured by some conservation organisations, did not work and cannot be recommended. The use of long‐term, continuously applied treatments might be considered for all perennial weeds with large underground root/rhizome systems.     

Glyphosate injury on Cyperus esculent us leaves and basal bulbs: histological study

The effect of glyphosate on leaves and basal bulbs of yellow nutsedge (Cyperus esculentus L.) plants was studied. Treatment with glyphosate did not affect leaf anatomy, although light necrotic spots were seen on the veins. In contrast, basal bulb organization was greatly affected with large necrotic zones and disorganization in the vascular cylinder due to herbicide accumulation. Depletion or disappearance of vacuolar phenols was observed in bulbs of plants treated with the highest doses of glyphosate. The most remarkable effect of glyphosate was the appearance of one layer of sclerenchymatic cells between root and rhizome primordia and the cortical tissues which could play an important role in the inhibition of rhizome and root emergence by the herbicide.     

Orobanche ramosa L. (broomrape) control in tomato (Lycopersicon esculentum Mill.) with chlorsulfuron, glyphosate and imazaquin

Chlorsulfuron, giyphosate and imazaquin were evaluated in pot and field studies for their efficacy in controlling broomrape (Orobanche ramosa L.) in tomato (Lycopersicon esculentum Mill.) in Northern Greece. All herbicides were applied four to five weeks after tomato transplanting, when the crop was at early flowering stage and broomrape had started to develop underground attachments. The number of emerged broomrape shoots and underground attachments were less affected by herbicide treatments than the dry weight, suggesting that the herbicides suppress the growth of broomrape rather than kill its underground organs. In the pot experiments, chlorsulfuron applied at 5 g AI ha^?1 was the most effective treatment for broomrape control and the least toxic to the crop. Imazaquin and glyphosate applied at 37 and 180 g AI ha^?1, respectively, controlled broomrape but imazaquin reduced crop yield. In the field, similar rates of glyphosate and higher rates of imazaquin were not toxic to the crop but were less effective on broomrape. Chlorsulfuron applied at 10 g AI ha^?1 controlled broomrape emergence by 88%. When the herbicide was applied twice (5+10 g AI ha^?1), it gave complete control of broomrape but delayed crop maturity. The yield of tomato was not increased as a result of these treatments because of low broomrape infestation and a short competition period.     

Selectivity of glyphosate in creeping red fescue and reed canarygrass*

The differential tolerance of resislant creeping red fescue (Fes- tuca rubra L, var, rutra) and susceptibie reed canarygrass (Pha- laris arundinacea L.) seedlings to glyphosate [N-(phosphono- meihyDglycine) was confirmed under growth chamber condilions. The absorption, transiocation and metabolism of ¹⁴C-glyphosate was examined in both species to determine if differences in these processes could account for the observed selectivity, Creepmg red fescue actually absorbed more glyphosate than did t-eed canarygrass, and both species rapidly translocated the herbicide throughout their respective tissues. No metabolism of glyphosate was detected in either species. Differential interception and retention of the glyphosate spray can probably be eliminated as possible selectivity mechanisms. The relative tolerance of creeping red fescue to glyphosale appears to be related to its ability lo regenerate roots and shoots from the crown of the plant, but the mechanism of resistance remains obscure.     

Growth of Parietaria debilis in fallow and in undisturbed areas

Parietaria debilis is a dominant annual weed in many non‐tillage fallow and undisturbed areas in Argentina. Herbicides control P. debilis inconsistently. A study on the growth of P. debilis, in a fallow in a soyabean–maize rotation including glyphosate‐resistant cultivars and in an undisturbed area without crops or weed control, was conducted in the central soyabean area of Argentina. The biomass dynamics of all species in the community, as well as biomass, density and growth traits of P. debilis were assessed. Biomass of several species including P. debilis and total biomass were greater in undisturbed than in fallow plots in both years of the study. Diversity was greater in undisturbed plots in 2000, but no differences in diversity were observed in 2001. In both treatments, P. debilis emergence began in March and finished in October and density increased from March to May. High mortality occurred in June and July, particularly in undisturbed plots. In the fallow plots, no other tall weed species were present and light interception was higher in spring when fruit production was at its peak. Conversely, in the undisturbed plots, P. debilis was substantially taller and had greater leaf area due to lower light interception by the high canopy of the rest of the community. In fallow, P. debilis is favoured by single applications of glyphosate, because the herbicide eliminates competitors. Control, if applied, should be directed to both fallow and undisturbed environments, as the weed produces considerable numbers of seeds and can invade extensively managed areas.     

Orobanche crenata (Forsk) control in Vicia faba (L.) with glyphosate as affected by herbicide rates and parasite growth stages

The broomrape (Orobanche crenata Forsk) susceptibility to glyphosate applied on faba-bean (Vicia faba L.) as affected by the parasite growth stages at the time of application was studied under field conditions. Glyphosate treatments delayed O. crenata emergence. Single glyphosate application to faba-bean infected with O. crenata predominantly at the stage (a) (small nodule) and (b) (nodules with initial vestigial roots) resulted in a moderate to low control. When the stages (c) (shoot bud already visible) or (d) (shoot and vestigial roots well developed) were the predominant stages, an excellent control was achieved with a single glyphosate application at 60 g ha^?1. Increased development stages, with the shoot emerged from the shoot bud, decreased its susceptibility to glyphosate. La lutte contre Orobanche crenata en culture de férerole avec le glyphosate; influence des doses d'herbicide et du stade de développement du parasite     

Absorption,translocation and metabolism of 14C-glyphosate in several weed species*

The pattern and extent of ¹⁴C-glyphosate [N-(phosphonomethyl)glycine] translocation from the treated leaf and metabolism of ¹⁴C-glyphosate were studied in field bindweed (Convolvulus arvensis L.), hedge bindweed (Convolvulus sepium L.). Canada thistle [Cirsium arvense (L.) Scop.] tall morning glory [lpomoea purpurea (L.) Roth.] and wild buckwheat (Polygonum convolvulus L.). ¹⁴C was translocated throughout the plants within 3 days with accumulation in the meristematic tips of the roots and shoots evident. Cross and longitudinal sections of stems and roots showed that the ¹⁴C was localized in the phloem. Field bindweed translocated 3–5% of the applied ¹⁴C from the treated leaf, hedge bindweed 21.6%, Canada thistle 7.8%, tall morningglory 6.5%, and wild buckwheat 5%. Field bindweed, Canada thistle, and tall morningglory metabolized the parent glyphosate to aminomethylphosphonic acid to a limited extent. This metabolite made up less than 15% of the total 14C. Of the total ¹⁴C applied to excised leaves, 50% had disappeared within 25 days.     

SHOOT ZONE UPTAKE OF SOIL-APPLIED HERBICIDES*

Summary. Studies were conducted to determine the effects of herbicide placement at different zones of maize (Zea mays L.) and pea (Pisum sativwm L.) shoots below the soil surface after emergence. Soil was removed from around the shoots and replaced with herbicide-treated soil. A wax barrier ensured separate exposure of the zones to treated soil. EPTC, chlorpropham, propham and sulfallate did not affect pea shoot growth, but in maize the shoot zone adjacent to the crown root node was extremely sensitive. Treatment in this area markedly reduced growth and severely inhibited the crown roots. The difference in susceptibility between these species may he due to the location of the growing point relative to the treated soil. Shoots of maize and pea were sensitive to diuron. In maize the shoot adjacent to the crown root node and the tissue of the first internode were the most susceptible. In pea the- uppermost shoot (beneath the soil surface) was the most sensitive. Trifluralin did not affect growth of maize and pea when placed in the shoot zone after emergence, although the crown roots of maize were severely inhibited. Naptalam, dalapon and 2,4-D did not affect growth of maize under similar conditions, and of these only 2,4-D reduced growth of pea. Zone d'abiorption des tiges pour les herbicides appliqués sur h sol     

Comparison of the effects of glyphosate and related compounds on indole-3-acetic acid metabolism and ethylene production in tobacco callus

Pretreatment of tobacco callus (Nicotiana tabacum L.) with glyphosate and related compounds promoted to a different degree the metabolism of radiolabeled indole-3-acetic acid ([2-¹⁴C]IAA) by increasing both conjugation and oxidation, and consequently lowered the level of free [2-¹⁴C]IAA. At identical concentrations, glufosinate ammonium was more active than glyphosate whereas glyphosine and aminomethylphosphonic acid were less active. These compounds also selectively reduced ethylene production and inhibited fresh-weight growth of tobacco callus. The activities of the tested compounds on IAA metabolism were correlated with those on inhibition of growth, suggesting that, under the experimental condition, the effect on IAA metabolism was a major contribution to their toxic action. This information may be useful for the design and testing of new herbicides and plant growth regulators of this type.     

Surfactant and salt affect glyphosate retention and absorption

The influence of nonylphenoxy surfactants and glyphosate salt formulation on spray retention, phytotoxicity and [¹⁴C]glyphosate uptake was investigated in wheat (Triticum aestivum L). and Kochia scoparia L. The amount of spray retained, and uptake of [¹⁴C]glyphosate increased with increasing hydrophilic-lipophilic balance (HLB) value of surfactants. The volume of spray delivered to the plant treatment area and retained by wheat and K. scoparia plants increased with increasing surfactant HLB values, but this only partly accounted for the higher spray retention. Spray retention by leaves of plants was not affected by calcium chloride, either alone or with ammonium sulphate in the glyphosate spray solution. [¹⁴C]Glyphosate absorption by wheat and K. scoparia was reduced by calcium chloride alone, but not in mixtures with ammonium sulphate, regardless of surfactant. Phytotoxicity and uptake of glyphosate salt formulations for wheat was: isopropylamine > ammonium > sodium > calcium; these results indicate that the surfactant selected is important to maintain glyphosate efficacy and that sodium and calcium cations antagonize glyphosate by forming salts that are absorbed less than commercial isopropylamine formulations.     

Low glyphosate rates do not affect Citrus limonia (L.) Osbeck seedlings

BACKGROUND: Glyphosate is used to control weeds in citrus orchards, and accidental spraying or wind drift onto the seedlings may cause growth arrest owing to metabolism disturbance. Two experiments were carried out to investigate the effect of non‐lethal rates (0, 180, 360 and 720 g AI ha^?1) of glyphosate on four‐month‐old ‘Cravo’ lime, Citrus limonia (L.) Osbeck, seedlings. Photosynthesis and the concentrations of shikimic acid, total free amino acids and phenolic acids were evaluated. RESULTS: Only transitory effects were observed in the contents of shikimate and total free amino acids. No visual effects were observed. CONCLUSION: The present study showed that glyphosate at non‐lethal rates, which is very usual when accidental spraying or wind drift occurs in citrus orchard, did not cause severe metabolic damage in ‘Cravo’ lime seedlings. Copyright © 2009 Society of Chemical Industry     

硫酸铵对草甘膦在空心莲子草中输导及除草活性的影响

草甘膦与硫酸铵混合后处理空心莲子草,测定其对植株的抑制作用和体内草甘膦的吸收与传导量。结果表明,加入硫酸铵(1.20 g/L)后草甘膦(300 mg/L)对地下根茎抑制率比对照提高了12.2个百分点。植株经硫酸铵喷雾处理后,地下茎和根系中14C-草甘膦含量分别是对照处理的1.39和1.86倍。药液中水的硬度达到342.0 mg/L时明显降低草甘膦的除草活性,硫酸铵浓度达到12.0 g/L则可基本消除水的硬度对草甘膦除草活性的影响。     

The addition of ammonium salts or a phosphate ester to herbicides to control Agropyron repens (L) Beauv.

N-(phosphonomethyl)glycine (glyphosate) at 0·5-1·0 kg a.i./ha controlled Agropyron repens grown in specially established 1-m² field plots in three out of four experiments. The addition of butyl acid phosphate or ammonium sulphate to the spray liquid improved the level of control given by glyphosate alone, where there was scope for such improvement: the addition of ammonium nitrate had no effect. When ammonium sulphate was added to either dalapon or asulam there was also no increased control of A. repens. However, when added to activated aminotriazole at 2·0 kg a.i./ha, ammonium sulphate significantly increased the level of control of A. repens in one experiment. Addition de sets d'ammonium ou d'un ester phosphorique à des herbicides en vue de la destruction de l'Agropyron repens (L) Beauv. La N- (phosphonométhyl) glycine (glyphosate) à al dose de 0.5–1.0 kg m.a./ha a été efficace contre l'Agropyron repens poussant au champ dans des parcelles spécialement établies de 1-m² dans trois essais sur quatre. L'addition de phosphate acide de butyle ou de sulfate d'ammonium au liquide pulvérisé a augments le niveau d'efflcacité obtenu avec le glyphosate seul, là oú il y avait possibilité de réaliser cette amélioration; l'addition de nitrate d'ammonium fut sans effet. Lorsque le sufate d'ammonium fut ajouté soit au dalapon, soit à l'asulame, il n'y eut pas non plus augmentation de l'efficacité contre A. repens. Toutefois, lorsque fut ajouté de l'aminotriazole activéà raison de 2 kg m.a./ha, le sulfate d'ammonium dans un essai accrut significativement le niveau d'efficacité contre A. repens. Der Zusatz von Ammoniumsalzen oder eines Phosphatesters zu Herbiziden zur Bekämpfung von Agropyron repens (L) Beauv. In drei von vier Versuchen wurde Agropyron repens in 1 m² grossen Feldparzellen durch 0.5–1.0 kg A.S./ha von N-(Phosphonomethyl)glycin (Glyphosate) bekämpft. Die Zugabe von Phosphorsäure-dibutylester oder von Ammoniumsulfat zur Spritzbrühe erhöhte die Wirkung, verglichen mit Glyphosate alleine. Der Zusatz von Ammoniumnitrat brachte keine Verbesserung. Auch wenn Ammoniumsulfat zu Dalapon oder zu Asulam zugesetzt wurde, wurde die Bekämpfung von A. repens nicht verbessert. Wenn aber zu ‘aktiviertem’ Aminotrizol (Aminotriazol+Ammonium-rhodanid) bei 2,0 kg A.S./ha Ammoniumsulfat zugegeben wurde, wurde in einem Versuch die Bekämpfung von A. repens deutlich verbessert.     

Uptake patterns of soil-applied 45Ca and 32P in some legume species as influenced by differential trifluralin placement

The effect of localized placement of trifluralin on uptake patterns of soil-applied ⁴⁵Ca in vetch (Vicia sativa L.), pea (Pisum sativum L.) and soybean (Glycine max) and ³²P in vetch and pea was investigated in two soil zones in the roots and in the shoot zone before and after plant emergence. When trifluralin was in the upper root zone severe inhibition of lateral roots occurred as well as a marked decrease in uptake of ⁴⁵Ca and ³²P from this zone. Root growth in the lower zone was unaffected, but uptake of ⁴⁵Ca and ³²P was slightly reduced. Compensatory adventitious root growth as well as a marked increase in uptake of ⁴⁵Ca and ³²P occurred in the shoot zone. Neither root growth nor uptake of ⁴⁵Ca or ³²P in the upper root zone were affected by the presence of trifluralin in the lower root region. When trifluralin was placed in the shoot zone after plant emergence, adven-titious roots on the shoots were inhibited and uptake of ⁴⁵Ca and ³²P was reduced.     

Influence of picloram on Cirsium arvense (L.) Scop, control with glyphosate

Low rates of picloram in mixture with glyphosate provided a rapid enhancement of the onset of injury to the shoots of Cirsium arvense (Canada thistle or creeping thistle) under field (0.07+1.0 and 0.07+1.5 kg ha^?1) and greenhouse (0.035+0.42 and 0.07+0.84 kg ha^?1) conditions. Picloram slightly reduced the amount of ¹⁴C-glyphosate absorbed at 24 and 48 but not 72 h after treatment. Movement of ¹⁴C-glyphosate from the treated leaves to the shoot apex, remainder of the shoot and roots was reduced in the presence of picloram. Necrosis of the treated leaves above the treated spots was evident, presumably indicating acropetal movement of either or both herbicides. With the picloram + glyphosate mixtures there was increased shoot regrowth over glyphosate alone at 1 year after treatment under field, and with certain mixtures at 18 days and 4 weeks after treatment under greenhouse conditions. Following application of the mixtures, accumulation of glyphosate in the shoots may be responsible for the enhanced onset of shoot injury while failure of enough glyphosate to translocate to, and cause death of, the roots may be responsible for the increased shoot regrowth over glyphosate alone.     

Studies in the growth of Pteridium aquilinum (L.) Kuhn (bracken). 2. Effects of shading and nutrient application

The effects of growing young plants of Pteridium aquilinum (L.) Kuhn (bracken) in shaded and unshaded conditions and with differential applications of nitrogen, phosphate and balanced fertilizer are described. The results are discussed in relation to possible effects on correlative inhibition. Single applications of N and P have little effect, but addition of both together or of NPK fertilizer produces increased frond production and rhizome growth. Shaded plants produce fewer fronds and these are of greater surface area, but thinner than those of unshaded plants. It is suggested that increased frond production resulting from enhanced nitrogen supply and exposure to full light may make bracken more susceptible to herbicide applications. Etudes sur la croissance de Pteridium aquilinum (L.) Kuhn (fougère). 2. Influence de l'ombre et de l'apport d'aliments Les conséquences pour la culture de jeunes plants de Pteridium aquilinum (L.) Kuhn (fougère) de conditions d'ombre ou de pleine lumiere avec des apports différentiels d'azote, de phosphate et de NPK sont mises en lumière. Les résultats obtenus sont considérés par rapport aux effets éventuels sur l'inhibition correlative. Des apports de N ou de P seuls ont eu peu d'influence mais les deux ensemble, de même qu'un apport NPK, ont amené la production de frondes plus nombreuses ainsi qu'une croissance plus importante des rhizomes. Les plantes cultivées à l'ombre produisent moins de frondes que celles en pleine lumiere; ces frondes ont une superficie plus étendue mais elles sont plus minces. Il se peut que la production de frondes plus nombreuses par des plantes en pleine lumière, et recevant des apports de N plus importants, rendent celles-ci plus sensibles aux applications herbicides. Untersuchungen über das Wachstum von Pteridium aquilinum (L.) Kuhn (Adlerfarn). 2. Effekt von Beschattung und Nährstoffzufuhr Es wird das Wachstum junger Pflanzen von Pteridium aquilinum (L.) Kuhn (Adlerfarn) unter beschatteten und unbeschatteten Bedingungen, sowie bei differenenzierter Zufuhr von Stickstoff, Phosphor und ausgewogenen Düngergaben beschrieben. Die Ergebnisse werden unter dem Aspekt möglicher Effekte auf korrelative Hemmungen diskutiert. N und P, einzeln appliziert, haben eine geringe Wirkung; beide zusammen angewandt oder die Verabreichung eines NPK-Düngers stimulieren das Wachstum von Wedeln und Rhizomen. Schattiert wachsende Pflanzen produzieren weniger Wedel. Diese haben jedoch eine grössere Oberfläche und sind dünner als diejenigen unschattierter Pflanzen. Es wird vermutet, dass eine verstärkte Wedelproduktion, unter dem Einfluss erhöhter N-Gaben und voller Lichtexposition, Adlerfarn gegenüber Herbizidapplikationen empfindlicher mechen kann.     

Effect of Pythium spp. and glyphosate on phytoalexin production and exudation by bean (Phaseolus vulgaris L.) roots grown in different media

Kievitone, phaseollinisoflavan and phaseollin were detected in roots of bean seedlings (Phaseolus vulgaris L.) grown in natural soil. Comparison of phytoalexin production by roots grown in different media indicated that these phytoalexins were probably induced by microorganisms in soil. The influence of common root rot pathogens of bean, Pythium spp., on phytoalexin production was determined. Pythium ultimum elicited kievitone, phaseollinisoflavan and phaseollin in roots grown in sterilized silica sand. P. sylvaticum induced only kievitone and phaseollin in the same growth medium. Glyphosate did not significantly affect the accumulation of phytoalexins within 3 days. However, by day 5, significantly more phaseollin was detected in the roots of Pythium inoculated plants treated with glyphosate than in Pythium inoculated plants not treated with glyphosate. In a hydroponic system, both Pythium spp. elicited accumulation of kievitone and phaseollin in root tissue, and both phytoalexins were exuded into the bathing solution. Glyphosate application did not significantly affect accumulation or exudation of phytoalexins by bean roots in the hydroponic system. The results from this study illustrate the nature and extent of phytoalexin production by bean roots in the absence and presence of microbes.