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利用染色体C 分带、基因组原位杂交和花粉母细胞减数分裂分析的方法 ,从普通小麦中国春与中国春 百萨偃麦草双倍体回交BC1F5 代中选育出 5份纯合易位新种质 ,分别为 :含 1对易位染色体并添加 1对百萨偃麦草完整染色体的Tj0 1和Tj0 2 ;添加 1对易位染色体的Tj0 3 ;含 1对易位染色体并添加 1对百萨偃麦草某一染色体臂端着丝粒染色体的Tj0 4和含 1对易位染色体并添加 2对百萨偃麦草完整染色体的Tj0 5。这些易位染色体的易位断点均不在着丝粒处 ,能稳定传递 ,且所涉及的植株生长和结实均正常。推测在该回交后代中小麦与百萨偃麦草染色体之间发生了自发的部分同源重组 ,这将有利于百萨偃麦草优异基因向普通小麦的转移与利用 相似文献
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With ass7istance of chromosome C-banding and genomic in situ hybridization(GISH)combined with meiotic analysis,five germplasms with homozygous wheat-Th. Bessarabicum chromosome translocations were developed and identified among BC1F5 progenies of the cross between T. Aestivum cv. Chinese Spring and Chinese Spring-Th. Bessarabicum amphiploid. These lines included Tj01 and Tj02(2n=44)containing a pair of wheat-Th. Bessarabicum translocation chromosomes besides a pair of added Th. Bessarabicum chromosomes,Tj03(2n=44)with a pair of added interspecific translocation chromosomes,Tj04(2n=44)containing a pair of interspecific translocation chromosomes besides an added pair of Th. Bessarabicum chromosome arms and Tj05(2n=46)containing a pair of interspecific translocation chromosomes besides two pairs of added intact alien chromosomes. The breakpoints of all the translocations were found to be not around centromere. Meanwhile,all the lines showed normal plant growth,development and fertility,while the translocation chromosomes transmitted regularly. The obtained translocations might be of use for transferring elite genes from Th. Bessarabicum into wheat. 相似文献
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小麦抗白粉病优质高产T1BL.1RS易位系的鉴定与分析 总被引：2，自引：1，他引：1
本文从创制的８０ 个新选系中鉴定出１２ 个抗白粉病品系,应用醇溶蛋白Ａ－ ＰＡＧＥ 检测发现其中９ 个为１ＢＬ／１ＲＳ易位系。麦谷蛋白亚基ＳＤＳ－ ＰＡＧＥ 分析表明,有３ 个含５ ＋ １０ 优质蛋白质亚基。综合农艺性状表现评定出９８ －１２３６ 和９８－ １２６６ 两个品系表现优异。进一步对籽粒主要品质性状检测表明,这两个品系均优于对照品种川麦２８ 号。 相似文献
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Zhang Xueyong Phillip M Banks P.J. Larkin 《中国农业科学》2000,(1):56-62
Z1, Z2, Z3, Z4, Z5 and Z6 are alien addition lines to wheat involving Thinopyrum intermedium chromosomes. We have characterized the Thinopyrum intermedium chromosomes or segments in these lines using multi-color florescence in situ hybridization. The probes used included total genomic DNA of Pseudoroegneria stipfolia (St) and cloned probes of highly tandem repetitive DNA pSc119. 2 and pAs1. Disomic addition lines Z1, Z2 and Z6 have the same single pair of alien chromo-somes carrying the resistant gene(s) to barley yellow dwarf virus (BYDV). This alien chromosome is a St/E translocation; within the long arm, there is a big insertion of an E-genome chromosomalsegment (30%). Disomic addition line Z3 carries one pair of St/E Robertsonian translocation chromosomes ; on the short arm (E) there is a nuclear organizer region, which expresses in some cells. In Z5, the added chromosome is one pair of translocated chromosomes. Chromosomes 2D, 3D and 3Stwere involved in the translocation with great possibility〔2IS · 3DL (0. 47) - 3StL (0. 53)〕. The St segment is responsible for resistance to leaf and stem rusts. Addition line Z4 also carries the translo cated chromosome found in Z5, but in addition carries one pair of 7AS (0. 64) - 7StS (0. 36) · 7StL translocation chromosomes. The 7St fragment bears the stripe rust resistance, and replaces the normal 7A. All of the translocations in Z1, Z2, Z6 and Z3 existed in one of their parents, the wheat Th. intermedium partial amphiploid, Zhong 5. The two wheat-Th. intermedium translocations in Z4 and Z5 occurred during the backcrossing of Zhong 5 to the other wheat varieties in the development of the addition lines. Spontaneous homoeologous translocations showed a close genome relationship between wheat and Th. intermedium. This paper also demonstrated the potential of highly repetitive sequences DNA in verification and characterization of translocation chromosomes. 相似文献
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从创制的 80个新选系中鉴定出 3个抗条锈病品系 ,应用醇溶蛋白A -PAGE检测发现其均为T1BL .1RS易位系。麦谷蛋白亚基SDS -PAGE分析表明 ,3个抗病易位系均含 5 + 10及 1或 2 优质蛋白质亚基。综合农艺性状表现评定认为 98- 12 6 6品系表现特别优异。其籽粒主要品质性状检测表明 ,该品系优于对照品种川麦 2 8号 相似文献