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1.
Cells were isolated from the developing leaves of Ipomoea aquatica (water spinach), a C3 plant, and three kinds of C4 plants, namely, Digitaria sanguinalis (NADP+-specific malate dehydrogenase type), Panicum miliaceum (NAD+-specific malic enzyme type), and Panicum texanum (phosphoenopyruvate carboxy kinase type), to study the effect of monuron on light-dependent 14CO2 fixation and oxygen evolution. Bundle sheath cells, except for those of D. sanguinalis, and mesophyll cells of all plants fixed approximately the same amount of 14CO2. Monuron, at the range used (2 to 10 × 10?7M), showed strong inhibition in the mesophyll cells of water spinach and in bundle sheath cells of P. miliaceum and P. texanum and moderate inhibition in the mesophyll cells of all C4 plants. In the bundle sheath cells of D. sanguinalis the low rate of 14CO2 fixation was stimulated to some extent by the addition of malate and ribose 5-phosphate. The I50 value was 6 × 10?7M for the sensitive cells. Monuron inhibited the oxygen evolution of all seven cell types and their I50 values varied between 3 × 10?7 to 6 × 10?7M. The differential response of isolated plant cells from different species to light-dependent CO2 fixation in the presence of monuron may also be involved in urea herbicide selectivity and undoubtedly is due to the differential photosynthetic pathways present nn them.  相似文献   

2.
Cells were isolated from the developing leaves of Ipomoea aquatica and Digitaria sanguinalis. The effects of phenoxy alkanoic acid herbicides on light-dependent 14CO2 fixation and oxygen evolution in these leaf cells were studied. (2,4-Dichlorophenoxy)acetic acid and (2,4,5-trichlorophenoxy) acetic acid (2,4,5-T and 2,4-D) caused a 20% stimulation of 14CO2 fixation at 0.8 × 10−5M and an inhibition at 1 × 10−4M in I. aquatica leaf cells. Temperature seemed to have a marked influence on such action. No effect or very little effect was observed in the leaf cells of D. sanguinalis. The nonactive (2,4,6-Trichlorophenoxy)acetic acid (2,4,6-T) caused a similar stimulation of CO2 fixation as 2,4-D and 2,4,5-T at low concentrations in I. aquatica leaf cells, but no inhibition was observed at high concentration. Increase of hight intensity increased the rate of CO2 fixation in both control and 2,4,6-T-treated cells; however, the percentage of stimulation remained the same. At stimulatory concentration, all three compounds did not cause any stimulation in either photosystem I and II or photosystem II-mediated oxygen evolution. At higher concentrations, the differential effects of 2,4-D and 2,4,5-T on the light-induced CO2 fixation and photosystem II-mediated oxygen evolution in the I. aquatica leaf cells and D. sanguinalis mesophyll (ms) cells may be attributed in part to their selective action against dicotyledonous plants.  相似文献   

3.
Using a set of representative members selected from 4-pyridone-3-carboxanilides and ortho-chlorinated diphenyl ethers exhibiting light-dependent herbicidal activity, we examined the three-dimensional structure-activity relationships quantitatively. The most stable conformation of each compound, regarded as the ‘active conformation’, was determined by either semi-empirical or ab initio molecular orbital calculations. With a hypothesis defining a common substructural skeleton between the two different compound series. each molecule was superimposed. We first analysed the structure-activity relationship using an index for the substructure shape similarity according to the superimposed conformations. After finding the relevance of the hypothesis, we examined the three-dimensional structure-activity relationship using the comparative molecular field analysis procedure. The results suggested that the two different series of compounds have a common region of the receptor site. The variations in the light-dependent herbicidal potency were governed by hydrophobicty and three-dimensional steric and electronic field parameters of molecules participating in the transport process and the interaction with the receptor site. The result was consistent with that derived from our previous quantitative analysis with the use of free-energy-related substituent parameters and the traditional regression procedure for a large number of pyridone-carboxanilides.  相似文献   

4.
Since their introduction in the early 1980s, there have been a number of field studies conducted to assess the effects of sulfonylurea herbicides on non-target plants (i.e. plants not labeled for use). In these studies a wide variety of plant response assessment techniques have been used to measure effects on non-target plants. This paper examines the relationship of short-term plant response measurements to plant productivity measurements such as yield or quality. Whether short-term plant response measurements have a practical degree of accuracy and precision appropriate for hazard assessment on non-target plants from sulfonylureas is discussed. A comprehensive review of published literature and unpublished field studies of the effects of sulfonylureas on the yield and quality of non-target plant species is reported. When this information is coupled with exposure factors and environmental fate characteristics, the risks to non-target plants from sulfonylureas are similar to those from other herbicides used at higher application rates. © 1998 SCI.  相似文献   

5.
Metabolism and selectivity of rice herbicides in plants   总被引:1,自引:0,他引:1  
Chemical weed control with effective and highly active herbicides has been very useful and convenient means. It has contributed to stable crop production and is labor saving. Recent herbicides have had characteristics such as high effectiveness without causing environmental pollution or harmful effects, and appropriate herbicides having high activity, low toxicity, high selectivity and being non-persistent have been developed. The metabolism of rice herbicides used mainly in Japan, such as sulfonylurea, chloroacetamide, acylamide, urea, thiocarbamate, pyrazole, triazine, diphenyl ether, phthalimide, phenoxy, aryloxyphenoxypropionate, etc., is reviewed, and its involvement in selectivity is also discussed. The metabolism of herbicides is closely related to their activity and selectivity. Differential herbicide metabolism in plants is a contributing factor of selectivity between crops and weeds. Chemicals that are more detoxified in crops and/or more activated or less detoxified in weeds are considered as being effective and selective herbicides. The metabolism of various types of rice herbicides includes: oxidative reaction (ring and chain hydroxylation, O - and N -dealkylation), hydrolysis and subsequent glucose conjugation, and glutathione conjugation in rice. These detoxicative activities are much higher in rice than weeds in paddies, and this leads to the selectivity of herbicides. Enzymes, oxidase, P-450 mono-oxygenase, esterase, acylamidase, glucosyl transferase, glutathione transferase, etc., play important roles in herbicide metabolism and selectivity.  相似文献   

6.
参照除草剂室内生物测定和田间药效防治试验准则,在温室中测定了14种除草剂对野稷的生物活性,并进行了田间小区药效评价。生测结果表明,土壤处理药剂中,异噁唑草酮、氟噻草胺、乙草胺、精异丙甲草胺、异噁草松对野稷的活性较高,其ED90分别为43.08、47.14、137.09、209.93、583.74 g/hm2(有效成分用量);茎叶处理药剂中,苯唑草酮、高效氟吡甲禾灵、烟嘧磺隆和苯唑氟草酮对野稷的活性较高,其ED90分别为22.21、35.95、95.64、111.43 g/hm2。田间药效试验结果表明,野稷出苗前,土壤喷雾40%氟噻草胺SC (810 g/hm2)、900 g/L乙草胺EC (1 620 g/hm2)和960 g/L精异丙甲草胺EC (2 160 g/hm2)对野稷防效较高,药后40 d对野稷的株防效均在86.0%以上,鲜重防效在88.4%以上,其次是75%异噁唑草酮WG (90 g/hm2),防效...  相似文献   

7.
The effects of the herbicides hexazinone [3-cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione] and chlorsulfuron (2-chloro-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl]benzenesulfonamide) on the metabolism of enzymatically isolated leaf cells from soybean [Glycine max (L.) Merr., cv. ‘Essex’] were examined. Photosynthesis, protein, ribonucleic acid (RNA), and lipid syntheses were assayed by the incorporation of specific radioactive substrates into the isolated soybean leaf cells. These specific substrates were NaH14CO3, [14C]leucine, [14C]uracil, and [14C]acetate, respectively. Time-course and concentration studies included incubation periods of 30, 60, and 120 min and concentrations of 0.1, 1, 10, and 100 μM of both herbicides. Photosynthesis was the most sensitive and first metabolic process inhibited by hexazinone. RNA and lipid syntheses were also inhibited significantly by hexazinone whereas the effect of this herbicide on protein synthesis was less. The most sensitive and first metabolic process inhibited by chlorsulfuron was lipid synthesis. Photosynthesis, RNA, and protein syntheses were affected significantly only by the highest concentration of this herbicide and longest exposure. Although these two herbicides may exert their herbicidal action by affecting other plant metabolic processes not examined in this study, hexazinone appears to be a strong photosynthetic inhibitor, while the herbicidal action of chlorsulfuron appeared to be related to its effects on lipid synthesis.  相似文献   

8.
Perfusion of 14C-(ring)-parathion or 14C-(ring)-paraoxon with blood through isolated, intact rat livers resulted in the rapid degradation of these insecticides. Degradation was negligible in the absence of rat liver (controls), thus demonstrating the capacity of the liver per se to effectively degrade these compounds. Of the total radiocarbon recovered after liver perfusion with [14C]parathion, 33 % could be attributed to unchanged [14C]parathion (similarly distributed between the liver and the blood) while 67.9 % was degraded to water soluble compounds and 2.5% was converted to organic soluble paraoxon and traces of p-nitrophenol. Nearly all of the [14C]paraoxon, however, was degraded by the intact rat liver, resulting in water soluble products that amounted to 98.5% of the total radiocarbon recovered. Unexplained losses of radiocarbon with the perfusion apparatus used were lower in the presence of rat liver which degraded the insecticides to more water soluble compounds. The water soluble degradation products produced from [14C]parathion and [14C]paraoxon were non-toxic to mosquito larvae (Aedes aegypti L.). These ring-labelled products were found to be conjugated p-nito-phenol. Nearly all of the water soluble radiocarbon was located in the perfused blood, while only small amounts (1.8 to 3.0% of recovered) were excreted via the bile or were associated with the liver tissue (1.3 to 1.8 % of recovered).  相似文献   

9.
大气CO2浓度增加对植物生长的影响   总被引:2,自引:0,他引:2  
就大气CO2浓度增加对植物光合、呼吸、蒸腾和水分利用效率、植物体内养分含量、生物量、产量的影响,及对C3、C4植物影响的差异等最新研究进展进行了分析综评,并就高CO2浓度与光照、温度、氮素营养水平等环境因子相互作用后植物生长、耐旱性、地理分布等问题进行了讨论。  相似文献   

10.
Substituted naphthoquinones, 2,3,-dichloro-1,4-naphthoquinone, and 2-methyl-1,4-naphthoquinone produced marked changes in the pattern of 14C-distribution during 14CO2-fixation by photosynthetic bacterium Rhodospirillum rubrum. The most obvious change in the labeling pattern during photoautotrophic 14CO2-fixation was a several-fold increase in 3-phosphoglyceric acid accompanied with a decrease in the amount of glutamate. In photoheterotrophic cells, quinones caused an appreciable increase in 14C-glycolic acid and concomitant decrease, although not proportional, in the amount of 14C-sugar phosphate. The level of 14C-incorporated in poly-β-hydroxybutyrate and ether-extractable lipids was considerably decreased in photoautotrophic and photoheterotrophic cells treated with quinones. The ability of quinones to interfere with the synthesis of NADH and ATP, and their ability to interact with sulfhydryl enzymes and coenzymes appears to be responsible for the changes observed.  相似文献   

11.
Several 2,4-dichlorophenoxyacetic acid (2,4-D)-sensitive plants have been modified by genetic engineering with tfdA gene to acquire 2,4-D tolerance. The expression product of this gene degrades 2,4-D to 2,4-dichlorophenol (DCP), which is less phytotoxic but could cause a problem of food safety. After a comparison of 2,4-D and DCP metabolism in transgenic 2,4-D-tolerant and wild cotton (Gossypium hirsutum L.), a direct study of DCP metabolism in edible plants was performed. After petiolar uptake of a [U-phenyl-(14)C]-DCP solution followed by a 48 h water chase, aqueous extracts were analysed by high-performance liquid chromatography. Metabolites were thereafter isolated and their structural identities were determined by enzymatic and chemical hydrolyses and mass spectrometry analyses. The metabolic fate of DCP was equivalent to 2,4-D metabolism in transgenic 2,4-D-tolerant cotton. In addition, DCP metabolism was similar in transgenic and wild cotton. The major terminal metabolites were DCP-saccharide conjugates in all species, essentially DCP-(6-O-malonyl)-glucoside or its precursor DCP-glucose. The significance of this metabolic pathway with regard to food safety is discussed.  相似文献   

12.
The effect of diquat and morfamquat on the fine structure of leaf ceils has been investigated in corn marigold (Chrysanthemum Segetum L.), flax (Linum usitatissitmum L. cv. Redwing), and barley (Hordeum vulgare L. cv unknown). Barley seedlings were exceedingly resistant lo morfamquat and appeared virtually unaffected by the treatments used. Chrysanthemum segetum and flax seedlings were sensitive and in both species treatment with herbicide at concentrations of 10?4M, or greater, caused swelling of the chloroplasts within 3–4 h. The change of shape of the chloroplasts continued until they were more or less spherical. Eventually the cell tonoplast and plasmalemma broke down and this was immediately followed by rupture of the chloroplast envelope. Other cell organelles disintegrated and. finally, all that remained in the cells were the membranous components of the chloroplasts and granular material from the nucleus. With flax the early effects also look place when the material was treated with these herbicides in the dark. Quelques effets du diquat et du morphamquat sur la structure fine des cellules foliaires L'effet du diquat et du morphamquat sur la structure fine des cellules foilaires a étéétudié chez le chrysanthéme des moissons (Chrysanthemum segetum L.), le lin (Linum usitatissimum L. ev Redwing) et l'orge (Hordeum vulgare L. cv inconnu). Les ptantules d'orge se sont montrées extrémement résistantes au morphamquat et n'ont pratiquement pas été affectés par les traitements effectués. Le Chrysanthemum segetum et les semis de lin se sont révé1és sensibles et, pour ces deux espéces, le traitement avec I'herbicide a des concentrations de 10?4 M OU plus, a provoqué le gonflement des chloro-plastes dans un intervalle de 3 ä 4 heures. Le changement de forme des chloroplastes se poursuivit jusquá ce qu'ils devins-sent plus ou moins sphériques. Par la suite, les membranes vacuolaires et les plasmalemmes se rompirent et ceci fut immédiatement suivi par la rupture de I'enveloppe du chloro-plaste. Les autres organelles cellulaires se desinttgrerent et finalement ne subsisterent dans les cellules que Ies constituants de la membrane des chloroplastes et le matériel granuleux provenant du noyau. Chez le lin, les premiers effets apparurent également lorsque le matériel fut traitéà I'obscurité avec ces herbicides. Einige Effekte con Deiquat und Morfamquat auf die Struktur von Blattzellen Es wurde die Wirkung von Deiquat und Morfamquat auf die Struktur der Blattzellen der Saat-Wucherblume (Chrysanthemum segetum L.), Flachs (Lintim tisitatissinium L.) und der Gerste (Hordeum itilgare L.,) untersucht. Die Gersten-sitmlinge waren gegenüber Morpham quat ausserordentlich resistent und wurden durch die Behandlungen unscheinend nicht beeinträchtigl. Die Sämlinge der Saat-Wucherblume und Flachs waren empfmdlich. Bei beiden Arten trat bei 10?4 molaren und höheren Herbizidkonzcntrationen innerhalb von 3–4 Stunden eine Schwellung der Chloroplasten auf. Die Formveränderung der Chloroplasten schritt fort, bis sie mehr oder weniger kugelformig waren. Schliesslich erfolgte der Zusammenbruch vonTonoplast und Plasmalemma, unmittelbar gefolgt vom Bruch der Chloroplastenmembran. Die anderen Zellorgaitellen lösten sich in ihre Bestandteile auf und was letztlich in den Zellen übrig blieb, waren Bestandteile der Chloroplastenmembran und granulöses Material vom Zellkern. Bei Flachs wurden die AnfangsePffekte auch dann beobachtet, wenn er mit diesen Herbiziden im Dunkeln behandelt wurde.  相似文献   

13.
Methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate (dichlofop-methyl) is a selective herbicide for wild oat (Avena fatua L.) control in wheat (Triticum aestivum L.). Dichlofop-methyl inhibited IAA-stimulated elongation of oat and wheat coleoptile segments by 51 and 13%, respectively, at 10 μM concentrations. Dichlofop-methyl alone had no auxin activity at concentrations of 0.1, 1.0, and 10 μM. The inhibitory effect of dichlofop-methyl was overcome partially by increasing the IAA concentration or by application of 3,6-dichloro-o-anisic acid (dicamba), a herbicide with weak auxin activity. The de-esterified free acid metabolite, 2-[4-(2,4-dichlorophenoxy)phenoxy]-propionic acid (dichlofop), at 10 μM inhibited auxin-stimulated oat coleoptile elongation by 23%, but it did not affect wheat coleoptile elongation at the same concentration. Both dichlofop-methyl and dichlofop inhibited root growth in excised shoots and seedlings of wild oat but had no effect on wheat. Dichlofop was a more effective inhibitor of root growth than dichlofop-methyl. The results suggest that dichlofop-methyl functions as a strong auxin antagonist, while the metabolite, dichlofop, inhibits root growth and development by another mechanism. The herbicidal effect of dichlofop-methyl may be the net effect of two biologically active forms of the compound each with a different mode of action acting at different sites within a susceptible plant.  相似文献   

14.
The effect of various herbicides on photosynthesis, respiration and transpiration of intact plants has been studied in a routine assembly. Simultaneous measurements were made under different experimental conditions in four small plant chambers, in which the shoots of various plant species can be accommodated. The herbicide is applied during measurement, so that the effect can be related to the photosynthetic activity of the same plants before treatment. The selectivity of various herbicides was studied by determining the capacity of a plant species to inactivate a herbicide absorbed by the roots. These and other differential effects of various herbicides on photosynthetic activity of different plant species coincide with the selective properties in the field. Such differences are also observed after leaf sprayings. The duration of the experiments is kept short. Bean plants were studied under various experimental conditions of air humidity, light intensity and temperature resulting in different transpiration rates. The decrease in photosynthetic activity owing to the presence of a herbicide in the nutrient solution at a standard concentration was more rapid at the higher transpiration rates. The total transpiration during treatment up to 50% inhibition of photosynthesis was constant under the various experimental conditions. Specific inhibitors of the photosynthetic process had a more pronounced effect on the photosynthetic activity than on transpiration rate. Some other herbicides affect transpiration as well as photosynthesis.  相似文献   

15.
The variations in the light-dependent herbicidal activity against Echinochloa oryzicola of a number of 4-pyridone-3-carboxanilides with various substituents on the anilide and pyridone rings have been examined by use of physicochemical substituent parameters and regression analysis. The effects of substituents of the anilide ring were such that the activity was related parabolically not only with the substituent hydrophobicity (π) but also with the steric bulk parameter (Es) of the bulkier ortho substituent. The effect of substituents at the 1-position of the pyridone ring was analysed with steric(STERIMOL L and B5) and electronic(γ1) parameters. That of substituents at the 5-position of the pyridone ring was explained by a parabolic function of a steric (MR) parameter. Analyses of the activity of 105 analogs confirmed that our previous selection of a candidate compound in this series for field trials was indeed appropriate in terms of physicochemical substituent effects.  相似文献   

16.
Sugar beet plants were grown in the field, after in-furrow application of [14C]aldicarb (3 kg of aldicarb ha?1) at planting. The ripe sugar beet plants were harvested, and the blades and petioles of the leaves were analysed separately. In the whole leaves, 15% of the 14C (all the percentages of 14C are relative to the total 14C incorporated into the whole leaves) was insoluble in ethanol+ water (1+1 by volume), 31% was organo-soluble (and thus unconjugated in the leaves), and 54% was water-soluble (mainly conjugated to plant constituents). The weights and concentrations (as aldicarb equivalents) of various identified metabolites of aldicarb, incorporated into the leaves, were determined; no aldicarb, as such, was detected.  相似文献   

17.
Sugar beet plants were grown in the field, after in-furrow application of [14C]- aldicarb (3 kg of aldicarb ha?1) at planting. The ripe sugar beet plants were harvested, and the roots were analysed. The roots were fractionated according to a procedure similar to the normal beet-sugar manufacturing process. Expressed as a proportion of the total radioactivity incorporated into the root, the pulp contained 29.7%, the lime cake 9.7%, the crystallised sugar 17.7% (which gave, with the radioactivity found in the sugar in the molasses, a total of 20.7% of the radioactivity in the total sugar), and the molasses, 42.9%. A part of the labelled carbon from the radio- active aldicarb and its metabolites had thus been metabolised and incorporated into sugar molecules. Except for the radioactivity in the sugar and in the lime cake from the processing, the proportion of radioactive non-conjugated organosoluble compounds was very low (2.6%), and perhaps partially corresponded to the very low amount of aldoxycarb (aldicarb sulphone) in the root (less than 0.001 mg of [14C]-aldicarb equivalents kg?1 fresh weight). Hydrolysis of the molasses yielded free radioactive 2-methyl-2-(methylsulphinyl)propan-1-ol (3.1%), 2-mesyl-2-methyl-propan-I-ol (8.9%) and 2-mesyl-2-methylpropionic acid (12.0%) which had been conjugated to plant constituents in the root. The corresponding concentrations (expressed as mg of [14C]aldicarb equivalents kg?1 fresh weight of root) were 0.004, 0.011, and 0.016, respectively. No aldicarb, aldicarb sulphoxide or aldoxycarb (nor the corresponding nitrile, generated from aldicarb during the fractionation procedure) was liberated by the hydrolysis, indicating the absence of conjugates of these compounds in the root.  相似文献   

18.
宋雪  王辉  孙延军  昝启杰 《植物保护》2021,47(4):269-275
为了筛选高效防除薇甘菊的除草剂,本文利用4种含灭草松或吡啶类的新型除草剂25%苄嘧磺隆·灭草松AS、25%滴酸·灭草松AS、25%滴酸·氨氯吡AS、25%氟胺·灭草松AS,以常用除草剂24%滴酸·二氯吡AS作对照,在深圳进行防除薇甘菊的试验研究.除草剂对薇甘菊防效结果表明:400 mL/hm2 25%滴酸·氨氯吡AS和...  相似文献   

19.
Sugar beet plants were grown in the field, after in-furrow application of [14C]aldicarb (3 kg of aldicarb ha?1) at planting. Some plants (the growing plants) were harvested 99 days after sowing and the rest (the ripe plants) 196 days after sowing. The percentages of the weights of [14C]aldicarb equivalents (the total aldicarb plus aldicarb sulphoxide and sulphone, plus all the other metabolites of [14C]aldicarb which contain 14C, expressed as aldicarb equivalents) incorporated into the beet plants, relative to the weight applied to the soil, were 2.8 and 1.8, respectively for the growing and ripe plants. The concentrations of [14C]aldicarb equivalents (mg kg?1 fresh weight) in the growing and ripe plants, respectively were: blades of the external leaves, 3.16 and 0.93; blades of the internal leaves, 0.63 and 0.68; petioles of the external leaves, 0.51 and 0.26; petioles of the internal leaves, 0.15 and 0.05; crowns, 0.14 and 0.15; roots, 0.16 and 0.13. The proportions of the extractable aldicarb plus aldicarb sulphoxide and aldicarb sulphone determined by gas-liquid chromatography (expressed as aldicarb equivalents) relative to [14C]aldicarb equivalents, in the external and internal leaf blades of the growing beets, were 56 and 60%, respectively; these values declined to 25 and 19%, respectively in the ripe plants. The proportion was 21 % or less in all other parts of the growing and ripe plants.  相似文献   

20.
几种除草剂防除马铃薯田杂草的效果   总被引:1,自引:0,他引:1  
25%砜嘧磺隆水分散粒剂对阔叶杂草和莎草防效较对禾本科杂草防效好;70%嗪草酮可湿性粉剂对马唐防效稍差,对其他杂草防效优秀;5%精喹禾灵乳油只对禾本科杂草防效优秀,对其他杂草无效果。23.2%砜嘧磺隆噁精喹禾灵·嗪草酮油悬浮剂综合了上述3个除草剂的优点,对马铃薯田禾本科杂草、阔叶杂草和莎草防除效果优秀。  相似文献   

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