Evaluating the impact of individual leaf traits on atmospheric particulate matter accumulation using natural and synthetic leaves

The ability of vegetation to capture and retain atmospheric Particulate Matter (PM) is directly dependent on the interactions between PM and plant surfaces. However, the impact of individual leaf traits in this respect is still under debate due to variations in published findings. This study employed standardised experimental designs with natural and synthetic leaves in three experiments to explore the impact of individual leaf traits on traffic-generated PM accumulation whilst other influential variables were controlled. The impact of leaf size on PM deposition was explored using synthetic leaves of different sizes (small, medium and large) but with the same shape and surface characteristics (n = 20 for each category). The impact of leaf shape was examined using another set of synthetic leaves of different shape (elliptical, palmately-lobed and linear) but with the same surface area and the same surface characteristics (n = 20 for each category). PM accumulation (PM₁, PM_2.5 and PM₁₀) on these leaves was quantified using an Environmental Scanning Electron Microscope (ESEM) and ImageJ software. Any differences in PM capture levels due to leaf size and leaf shape were identified using one-way Anova and Tukey’s pairwise comparison. In a subsequent experiment, equal-sized, square-shaped leaf sections obtained from four plant species (n = 20 for each species) with different micromorphology were exposed to traffic-generated pollution and any PM capture differences due to leaf micromorphology identified employing the same SEM/ImageJ and statistical approach. The results of all three experiments showed significant differences in PM accumulation between different leaf sizes (p < 0.001), between different leaf shapes (p < 0.001) and between different leaf micromorphology (p < 0.001) suggesting that all these characters are influential in the capture and retention of PM on leaves. Smaller leaves and complex leaf shapes (lobed leaves) showed a greater potential to capture and retain PM. Leaf surfaces with hair/trichomes, epicuticular wax, and surface-ridges accumulated more PM compared to smooth surfaces; of these characters, leaf hairiness/presence of trichomes was found to be the most important. Species sharing most of these important leaf traits are recommended as effective PM filters.     

Particulate matter pollution capture by leaves of seventeen living wall species with special reference to rail-traffic at a metropolitan station

Atmospheric Particulate Matter (PM) constitutes a considerable fraction of urban air pollution, and urban greening is a potential method of mitigating this pollution. The value of living wall systems has received scant attention in this respect. This study examined the inter-species variation of particulate capture by leaves of seventeen plant species present in a living wall at New Street railway station, Birmingham, UK. The densities of different size fractions of particulate pollutants (PM₁, PM_2.5 and PM₁₀) on 20 leaves per species were quantified using an Environmental Scanning Electron Microscope (ESEM) and ImageJ image-analysis software. The overall ability of plant leaves to remove PM from air was quantified using PM density and LAI (Leaf Area Index); any inter-species variations were identified using one-way Anova followed by Tukey’s pairwise comparison. This study demonstrates a considerable potential for living wall plants to remove particulate pollutants from the atmosphere. PM capture levels on leaves of different plant species were significantly different for all particle size fractions (P < 0.001). Smaller-leaved Buxus sempervirens L., Hebe albicans Cockayne, Thymus vulgaris L. and Hebe x youngii Metcalf showed significantly higher capture levels for all PM size fractions. PM densities on adaxial surfaces of the leaves were significantly higher compared to abaxial surfaces in the majority of the species studied (t-test, P < 0.05). According to EDX (Energy Dispersive X-ray) analysis, a wide spectrum of elements were captured by the leaves of the living wall plants, which were mainly typical railway exhaust particles and soil dust. Smaller leaves, and hairy and waxy leaf surfaces, appear to be leaf traits facilitating removal of PM from the air, and hence a collection of species which share these characters would probably optimize the benefit of living wall systems as atmospheric PM filters.     

Seasonal changes in CO2 assimilation in leaves of five major Greek olive cultivars

Leaf CO₂ assimilation rate, stomatal conductance (g_s), internal CO₂ concentration (C_i), chlorophyll (a + b) content, specific leaf weight (SLW) and stomatal density were measured during the season, under field conditions, for five major Greek olive cultivars, ‘Koroneiki’, ‘Megaritiki’, ‘Konservolia’, ‘Lianolia Kerkiras’, and ‘Kalamon’, with different morphological and agronomic characteristics and diverse genetic background. Measurements were taken from current-season and 1-year-old leaves, and from fruiting and vegetative shoots, throughout the season, from March to November in years 2001 and 2002. CO₂ assimilation rates showed a substantial seasonal variation, similar in all cultivars, with higher values during spring and autumn and lower values during summer and late autumn. Stomatal conductance (g_s) followed similar trends to leaf CO₂ assimilation rates, increasing from March to July, following by a decrease during August and increasing again in autumn. ‘Koroneiki’ had the highest leaf CO₂ assimilation rate and g_s values (21 μmol m⁻² s⁻¹ and 0.45 mol m⁻² s⁻¹, respectively) while ‘Lianolia Kerkiras’ and ‘Kalamon’ showed the lowest leaf CO₂ assimilation rate and g_s values (13–14 μmol m⁻² s⁻¹ and 0.22 mol m⁻² s⁻¹, respectively). One-year-old leaves had significantly higher leaf CO₂ assimilation rate than current-season leaves from April to June, for all cultivars. From August and then, leaf CO₂ assimilation rate in current-season leaves was higher than in 1-year-old leaves. There were no significant differences in leaf CO₂ assimilation rate between fruiting and vegetative shoots. Total chlorophyll (a + b) content increased with leaf age in current-season leaves. In 1-year-old leaves chlorophyll content increased in spring, then started to decrease and increased slightly again late in the season. Chlorophyll content was higher in 1-year-old leaves than in current-season leaves throughout the season. Total specific leaf weight (SLW) increased throughout the season for all cultivars. Stomatal density in lower leaf surface was lowest for ‘Koroneiki’ (399 mm⁻²) and highest for ‘Megaritiki’ (550 mm⁻²). Our results showed differences in leaf CO₂ assimilation rate among the five different olive cultivars, with a diverse genetic background, ranging from 12 to 21 μmol m⁻² s⁻¹. From the five cultivars examined, ‘Koroneiki’, a drought resistant cultivar, performed better and was able to maintain higher leaf CO₂ assimilation rate, even under high air vapor pressure deficit. All cultivars had a pronounced seasonal variation in leaf CO₂ assimilation rate, affected by date of the year, depending on ambient conditions. The high temperatures and high air vapor pressure deficit occurring during summer caused a reduction in leaf CO₂ assimilation rate in all cultivars. Leaf CO₂ assimilation rate was also affected by leaf age for all cultivars, with old leaves having significantly higher leaf CO₂ assimilation rate than young leaves early in the season.     

Effect of the elevated ozone on greening tree species of urban: Alterations in C-N-P stoichiometry and nutrient stock allocation to leaves and fine roots

Anthropogenic ground-level ozone (O₃) pollution can alter the phosphorus (P), carbon (C), and nitrogen (N) of terrestrial plants’ ecological stoichiometry, which in turn affects forest productivity, nutrient utilization, and carbon sink capacity. However, there is still quite a lot of uncertainty regarding the impact of high O₃ levels on C-N-P stoichiometry in organs with a rapid turnover (i.e., fine roots and leaves) across varied functional types. This study investigated the effects of O₃ on the stoichiometry of C-N-P nutrient allocation of stocks to various plant organs, with a special focus on tree species frequently employed for urban greening. The impact of O₃ on C-N-P stoichiometry among different functional tree types was subsequently evaluated by reviewing the published literature. Under a pooling of all species, elevated O₃ decreased and leaf C and P concentrations increased, thereby decreasing the leaf C: P ratio. Elevated O₃ increased the N concentration in fine roots, thereby decreasing the C: N ratio, although no significant impact was observed in leaves. Elevated O₃ significantly reduced the leaf stocks of C (CS_leaf) and N (NS_leaf), however, there was no observed variation in these stocks in fine roots. The content of P, C, and N in fine roots and leaves in evergreen broadleaf species exceeded those in deciduous species. Elevated O₃ significantly reduced CS_leaf, NS_leaf, and PS_leaf in deciduous broadleaf species, whereas there was a significant reduction for the same in evergreen species. The literature analysis further demonstrated a larger O₃-induced increment in leaf P concentration in deciduous species as compared to evergreen species. Elevated O₃ significantly increased the difference in C and N stocks between fine roots and leaves in deciduous broadleaf species, whereas this difference was observed to decrease in evergreen species. The results of this study can facilitate an improved understanding of ecological stoichiometric responses of urban greening tree species under O₃ stress and the resulting nutrient use strategies.     

A simple method for non-destructive estimation of total shoot leaf area in tree fruit crops

Leaf area is a commonly used measurement in many horticultural research experiments, but is generally destructive, requiring leaves to be removed for measurement. Leaf removal precludes repeated measures and disrupts carbohydrate assimilation and translocation, which is another frequent parameter of interest. Numerous methods have been devised for estimating leaf area of individual leaves in situ. These methods generally involve measuring leaf parameters such as length and width and using those as variables in an equation describing a relationship between those variables and leaf area specifically developed for each species. However, these methods are typically highly labor intensive when scaling to the level of the shoot because they require each leaf to be measured individually. Here we report on a method that makes use of a regression relationship for non-destructively estimating whole shoot leaf area, but only involves measuring the length of the longest leaf on the shoot and multiplying it by the number of leaves on the shoot. The method has been tested on 14 diverse fruit and nut tree cultivars. Deciduous and evergreen species with both simple and compound leaves were included. For 13 out of 14 cultivars, coefficients of determination (r²) between actual measured leaf area and estimated leaf area were equal to or greater than 0.80. The model was validated for shoot leaf area of different trees in different years for four of the cultivars. The relationship developed for these cultivars appears to be broadly applicable to other tree species and will be a simple and useful tool for research requiring accurate non-destructive estimates of shoot leaf area.     

Mechanisms of salt stress tolerance in two rose rootstocks: Rosa chinensis ‘Major’ and R. rubiginosa

The responses of two rose rootstocks Rosa chinensis ‘Major’ and R. rubiginosa were investigated under salt stress. The distribution of chloride and sodium ions in all plant parts was determined. The salt treatments were applied through irrigation water containing 0, 5, 10, 20 and 30 mM NaCl. Necrosis on the leaves as a result of the NaCl treatments was observed with in rootstocks after two months. Leaf injury was more pronounced in R. chinensis ‘Major’ than R. rubiginosa. The rootstock R. rubiginosa showed a higher tolerance to the NaCl stress than R. chinensis ‘Major’. The survival of the plants under increased NaCl stress as well as the extent of leaf injury could be used in the determination of tolerance of the rose genotypes. The lower older leaves contained higher concentrations of Cl⁻ than the young upper leaves. Leaf samples had higher concentrations of Cl⁻ than stem samples taken from the same positions. The roots contained higher amounts of Cl⁻ than the stem samples. The plants accumulated higher amounts of Cl⁻ in comparison with Na⁺. The lower leaves of R. chinensis ‘Major’ had higher amounts of Na⁺ than in all other parts whereas R. rubiginosa had higher concentrations of Na⁺ in the roots than in all other parts.     

Predicting dry matter production of cauliflower (Brassica oleracea L. botrytis) under unstressed conditions: I. Photosynthetic parameters of cauliflower leaves and their implications for calculations of dry matter production

Measurements of CO₂ exchange of cauliflower leaves were carried out in a field experiment which included two nitrogen fertilisation rates. Irradiance and CO₂ concentration were varied at the leaf level within a leaf cuvette and additionally a temperature treatment was applied to field grown plants moved into climate chambers. These measurements were used to estimate parameter values of a rectangular hyperbola describing cauliflower leaf CO₂ exchange as a function of irradiance and CO₂ concentration. The obtained parameter estimates were used to derive empirical regression equations with temperature and nitrogen content of the leaves as independent variables. The resulting relationships were applied within a simple photosynthesis–respiration based dry matter production model in order to derive functional relationships between light use efficiency and irradiance, leaf area index and temperature.The rectangular hyperbola was able to describe the gas exchange data as varied by irradiance and CO₂ concentration on the single leaf level with sufficient accuracy, but estimates of initial light use efficiency (about 25 μg J⁻¹) were too high because of the bias emanating from the limited flexibility of this model. Light saturated photosynthesis rate (P_max) showed an optimum response to temperature and an increase with increasing nitrogen content of leaves. The initial slope α of the rectangular hyperbola showed no consistent responses to ambient temperature and nitrogen content of leaves. The respiration per unit leaf area β increased exponentially with increasing temperature, resulting in a Q₁₀ value of 1.86. Because only a limited number of plants was evaluated in this study, additional work is needed to further substantiate the results of the gas exchange measurements.The model analysis demonstrated that LUE is independent of the light integral over a range 5–10 MJ m⁻² per day photosynthetically active radiation if one assumes an adaptation of P_max within the canopy and over time according to the incident irradiance. Acclimatisation within the canopy and higher leaf area indices, LAI, reduce the decrease of LUE with irradiance but a substantial decline remains even for LAI values of 4.     

Effects of simulated acid rain on the foliage and fruit yield of Malm domestica Borkh.

The effects of rain acidity on field-grown apple trees (Malus domestica Borkh. cv. Sum- merred) under natural conditions were investigated. One group of four trees was exposed to ambient rainfall. Four other groups were covered with rain-shields and for two growing seasons received simulated rain consisting of deionized water or an acid solution at pH 5.6,4.0 or 3.0 respectively. Simulated acid rain, particularly at pH 3.0, injured leaves and petals, reduced fruit production and impaired marketability. SEM examination showed that damage to the leaves (necrosis and lesions) was most extensive at the moment of full leaf expansion, whereas damage to the fruit (russeting and lesions into the mesocarp) was most severe when the fruit was almost fully ripe. The reduction in production was 19% with the pH 4.0 spray and 37% with the pH 3.0 spray (compared with rainfall-fruit). The acidity of the ambient rainfall encountered in this study caused spotting and necrosis of the leaves but did not significantly affect fruit production.     

Essential and non-essential element composition of tomato plants fertilized with poultry manure

Poultry manure (PM) must be disposed of from poultry farms, but is a potentially valuable source of macro- and micronutrients for plant growth. The objective of this study was to examine the effects of poultry manure on the growth of tomato (Lycopersicon esculentum) plants. Yields of fruits and vegetative material of plants grown in soil with 0, 10, 20 and 40 g kg⁻¹ PM added were measured. Concentrations of N, P, K, Ca, Mg, S, Fe, Zn, Cu, Mn, Mo, Cl, Si, Br, Rb, Sr and Ba in leaves at flowering and at final harvest and in fruits were determined by polarized energy dispersive X-ray fluorescence (PEDXRF). Poultry manure fertilization improved tomato shoot growth and also fruit yield and increased leaf N concentrations at the harvest stage. In addition, P concentrations of the leaves and fruits were increased as the application rate of PM was increased. Fruit Ca and Mg were significantly reduced by increased rate of PM application, but not to the extent to cause the calcium deficiency disorder blossom end rot. Applied high levels of PM slightly increased the concentrations of leaf Mo and Br at the harvest stage. Poultry manure applications had a positive effect on the concentrations of leaf Zn, Cu, Cl and Rb at both sampling stages, but leaf Si concentration was reduced by PM treatments. The concentrations of Zn and Rb were increased in the fruits by PM treatments, but the concentrations of Br were decreased. Applied PM levels had no significant effects on the concentrations of K, S, Fe, Sr or Ba in tomato plants. It is concluded that the increased fruit yield, and the increased concentration of Zn (an element required in the human diet) and the lowered concentration of potentially harmful Br in the fruit make poultry manure a valuable growing medium for tomato production.     

Air pollution removal through deposition on urban vegetation: The importance of vegetation characteristics

Urban vegetation has the potential to improve air quality as it promotes pollutant deposition and retention. Urban air quality models often include the effect vegetation have on pollution dispersion, however, processes involved in pollution removal by vegetation are often excluded or simplified and does not consider different vegetation characteristics. In this systematic review, we analyze the influence of the large interspecies variation in vegetation characteristics to identify the key factors affecting the removal of the major urban pollutants, particulate matter (PM) and nitrogen dioxide (NO₂) from the air through vegetation deposition. The aim is to identify key processes needed to represent vegetation characteristics in urban air quality modelling assessments.We show that PM is mainly deposited to the leaf surface, and thus representation of characteristics affecting the aerodynamics from canopy down to leaf surface are important, such as branch/shoot complexity and leaf size, leaf surface roughness and hairiness. In addition, characteristics affecting PM retention capacity, resuspension and wash-off, include leaf surface roughness, hairiness and wax content. NO₂ is mainly deposited through stomatal uptake, and thus stomatal conductance and its responses to environmental conditions are key factors. These include response to solar radiation, vapour pressure deficit and soil moisture.Representation of these vegetation characteristics in urban air quality models could greatly improve our ability to optimize the type and species of urban vegetation from an air quality perspective.     

Monitoring of spatial variations of particulate matter (PM) pollution through bio-magnetic aspects of roadside plant leaves in an Indo-Burma hot spot region

Particulate matter (PM) is an important air pollutant because of its adverse impacts towards human health. The existing and conventional methods of PMs monitoring are found to be inadequate in feasibility, which paved the way of magnetic biomonitoring approach. The magnetic measurement carried through the plant leaves is useful means in assessing the PM pollution. Plant species are found to be an effective biomonitors and may act as natural filters by trapping and retaining the PM on their leaf surfaces. Therefore, the aim of this communication is to demonstrate the magnetic properties [magnetic susceptibility (χ), anhysteretic remanent magnetization (ARM) and saturation isothermal remanent magnetization (SIRM)] of two roadside plant leaves (Hibiscus rosa-sinensis and Mangifera indica) at four spatially distant sites. The study measures and compares the capabilities of these plants to accumulate and retain the PMs. The study also assesses the PM pollution at selected sites and establishes the relationship between magnetic properties and PM in the city of an Indo-Burma hot spot region. The results indicated a significant correlation between the concentration of ambient PM and magnetic measurement (χ, ARM and SIRM) of both the roadside plant leaves. Similarly, reasonably good correlations are obtained between magnetic parameters (χ, ARM and SIRM) and Fe content in PMs. Present study is, perhaps, a novel contribution in the area of bio-magnetic monitoring studied with several magnetic parameters viz., χ, ARM and SIRM. Results indicated that the bio-magnetic monitoring is applied for environmental geomagnetism which act as proxy for ambient PM pollution and further employed as an eco-sustainable tool for environmental management in urban and peri-urban regions.     

Analysis of xylem water as an indicator of current chloride uptake status in citrus trees

The potential use of stem xylem chloride (Cl⁻) analysis as an indicator for sudden variation in Cl⁻ uptake by the tree was examined. Three extraction techniques (xylem sap extracted by centrifuge, and water extraction of fresh or dry ground xylem) were linearly correlated to each other as well as to soil salinity. Chloride values in xylem sap extracted by centrifugation were about half (3–34 mM) of those found in water extraction of fresh or dry ground xylem (9–84 mM), reflecting some Cl⁻ accumulation by existing living cells. Based on dry ground xylem, xylem Cl⁻ concentration was not affected by time of day; however, when centrifuge extraction was used, daily values were highest at the beginning and end of the day, and lowest during mid-day. Additionally, Cl⁻ concentration was not affected by stem thickness (5–25 mm) using the dry ground extraction method. Rootstock affected xylem Cl⁻ content in the same way it affected leaf Cl⁻ content; xylem Cl⁻ content was highest for trees grafted on salt-sensitive rootstocks and vice versa. As opposed to leaf analysis in which Cl⁻ concentration can only increase with time, xylem Cl⁻ content always followed soil salinity variation, either increasing or decreasing. Thus, analysis of stem xylem water can be a valuable tool for detecting short-term variation in Cl⁻ uptake. This method is important in trees since leaf analysis reflects the cumulative Cl⁻ content and therefore does not always give the current transitory mineral uptake status.     

Testing the suitability for coastal green areas of three ornamental shrub species through physiological responses to the saline nebulization

Besides abiotic constraints, plants along the coastal urban areas must face additional cues such as saline aerosol, which impact net plant CO₂ assimilation (P_n), reducing biomass and influencing their aesthetic features. In this study, three species (Photinia × fraseri, P; Escallonia rubra, E; and Feijoa sellowiana, F) were subjected to saline nebulization (SN) with a 100 mM NaCl solution. Analyses were performed at 0, 10, and 20 days by monitoring the ion accumulation in plant organs, leaf osmotic potentials, gas exchange, chlorophyll a fluorescence parameters, and chlorophyll contents. Overall, E-SN plants absorbed more Na⁺ and Cl⁻ in leaves than P-SN and F-SN ones. This phenomenon was influenced by leaf ‘wettability’ features such as the contact angle of water droplets, droplet retention, and water storage capacity, and the effectiveness of translocating these ions on twig tissues. SN increased the leaf osmotic potential (regardless of species). At 10 days (i.e., moderate stress conditions), P_n declined in all SN species, but more severely (−82 %) in E-SN plants. The observed P_n reductions were due to different limiting factors according to the plant species: P_n was reduced by non-stomatal limitations in P-SN plants, stomatal closure in F-SN, and a combination of both in E-SN individuals. At 20 days (i.e., severe stress conditions), in all SN-plants, lower values in all the physiological parameters than controls were observed, indicating a low tolerance to prolonged SN. The work shows that non-destructive physiological measurements provide a reliable assessment of plant tolerance to SN, which can help growers to select ornamental species suitable for coastal green areas.     

Plant establishment on a green roof under extreme hot and dry conditions: The importance of leaf succulence in plant selection

Plant selection for extensive green roofs has largely been based on cool, temperate climate research. However, as green roof implementation in hotter and drier climates increases, there is a need to evaluate plant performance under these climatic conditions. Succulents have been shown to be successful in hot and dry green roofs, although survival differs between species and the role of leaf succulence in survival has not been fully explored. For non-succulent plants, habitats with conditions similar to green roofs (‘habitat templates’) have been used to select plants, although few studies have discussed the performance of these selections under green roof conditions. Therefore, we evaluated establishment of 32 plant species on an unirrigated extensive (125 mm deep) green roof in Melbourne, Australia over a 42 week period (from winter through summer into autumn). Plants were selected on the basis of life-form, succulence, appropriate habitat templates and/or successful use on green roofs internationally. Climatic conditions during the experiment were often extreme, with evaporation regularly exceeding rainfall and a hot and dry summer (mean maximum air temperature 35 °C and 80.6 mm total rainfall), leading to roof temperatures of 65 °C. After 42 weeks, only succulent plants remained alive and only three of the succulent species had 100% survival. Survival was positively related to the degree of leaf succulence (g H₂O leaf area cm⁻¹) making this a useful trait for plant selection for unirrigated green roofs in hot, dry climates. The failure of most species, despite being chosen from appropriate habitats, demonstrates the need to evaluate potential plants on green roofs under extreme climatic conditions. Supplementary irrigation may be essential to sustain non-succulent species during extreme weather in hot and dry climates.     

Geometrical analysis of development of erect leaves as a factor in head formation of Brassica rapa L.: (I) Geometrical change of growing leaves in head cultivars

A geometric model simulating the hyponastic bend of the basal part of the midrib was developed to investigate factors involved in head formation of Chinese cabbage (Brassica rapa L. var. pekinensis Rupr.). The model, named the ‘double-truncated-cone model’, considers the midrib as the side face of inversely connected truncated cones. The convex side face simulates the bend of midrib. The bend angle of the midrib calculated using the model was highly correlated with the actual bend angle over various leaf growth stages. Thus, the model approximately accounted for the bend that induces development of erect leaves. Analysis of midrib growth using the model suggests that development of erect leaves is induced by midrib bend mainly caused by the following dimensional changes in the midrib: (1) an increase of (l₁ − l₀)d₁⁻¹, where l₁, l₀, and d₁ are the length of the cross-sectional arc at the bend position, that at the basal end of midrib, and the distance from the basal end to the bend position, respectively; and (2) a decrease of the central angle of the cross-sectional arc.     

Indicators of nitrogen status for ornamental woody plants based on optical measurements of leaf epidermal polyphenol and chlorophyll contents

Indicators of plant nitrogen (N) status adapted to woody ornamental plants are essential for the adjustment of fertilization practices in nurseries. The objective of this study was to investigate whether optical measurements of leaf epidermal polyphenol (EPhen) and chlorophyll (Chl) contents could be used as N status indicators for woody deciduous and evergreen ornamental plants. One-year-old plants of Lagerstroemia indica, Callicarpa bodinieri and Viburnum tinus were grown outdoors in containers. They received low (TN₁, 4 mg L⁻¹) or high (TN₂, 105 mg L⁻¹) levels of N during 2 months in spring and summer. TN₁ treatment limited shoot growth from 28 to 37 days after treatment initiation in Lagerstroemia and Callicarpa, respectively. Shoot growth was unaffected until day 176 in Viburnum. The mass-based leaf N content (N_M) of a sample of young expanded leaves exposed to direct sunlight was tightly correlated with shoot N content and differentiated treatments several weeks before shoot growth reduction for the three species. N_M was therefore used as an index of plant N status. EPhen and Chl contents were recorded with Dualex™ and SPAD-502 leaf-clip meters, respectively. Dualex values were strongly and negatively correlated with N_M, and differentiated the treatments early in the experiment, in all three species. SPAD values were positively correlated with N_M for Lagerstroemia and Callicarpa, but not for Viburnum, because large variations in leaf mass per area (LMA) in this species compensated for variations in leaf dry mass invested in Chl. The SPAD/Dualex ratio was used to assess changes in the proportion of leaf dry mass allocated to proteins and polyphenols in response to fertilization. It differentiated between the treatments early in the experiment and was correlated with N_M in all three species.     

Seasonal variations in leaf gas exchange of plantain cv. Hartón (Musa AAB) under different soil water conditions in a humid tropical region

The seasonal effect of soil water availability on leaf gas exchange of plantain plants cv. Hartón growing on two different texture soils (loamy and clayey) were evaluated. Soil water deficits corresponded to 48, 24 and 4 days without precipitation. Daily measurements of leaf gas exchange and microclimatic conditions were carried out at 2 h intervals in a humid tropical environment south of Maracaibo Lake, Venezuela. The results show that cv. Hartón is sensitive to conditions of low water deficit on loamy and to a much greater degree on clayey soils. A marked reduction in leaf conductance (g_s) was observed under severe as well as moderate deficit (below 50 mmol m⁻² s⁻¹) on clayey soils. Under low deficit g_s increases to values between 60 and 100 mmol m⁻² s⁻¹. The same trend was observed in plants on loamy soils but higher g_s for all conditions were obtained compared with plants on clayey soil. Stomatal closure produced a reduction of 85 and 55% of total assimilation (A_tot) for severe and moderate deficit in plants on clayey soils, respectively. While plants on loamy soil exhibited a 65 and 35% reduction, respectively. Water use efficiency (WUE) consistently decreased as available soil water decreased on both soil types. Independently of soil water conditions, higher WUE were always obtained for loamy soils. This suggests that cv. Hartón does not have the ability to adjust the CO₂ assimilation to transpiration ratio in order to optimize gas exchange. This evidences the importance of maintaining high conditions of available soil water in order to avoid lower assimilation rates that probably influence negatively on yield and fruit quality.     

Effect of gibberellin inhibitors on growth and mineral nutrition of sour orange seedlings

Sprays of gibberellin inhibitors (GI) paclobutrazol, XE 1019 or flurprimidol at 1000 mg 1⁻¹ (active ingredient) to sour-orange seedlings (Citrus aurantium L.) reduced total (terminal + lateral) shoot growth, lateral growth, dry weight, leaf area per plant, area per leaf and leaf number, and increased root to top dry weight ratio. The magnitude of response to paclobutrazol and XE 1019 was similar, but greater than to flurprimidol. Branching was not affected by any GI's. Root length was reduced by all GI's, but the magnitude of response was similar with all chemicals. The effect of sprays on shoot growth lasted 7 weeks. Re-wetting of the plant surface induced recurrence of GI activity. Multiple sprays prolonged the inhibitory effect of chemicals on shoot growth. Flurprimidol and XE 1019 reduced leaf Cu level, and XE 1019 increased leaf Mg. Increasing the concentration of sprays from 1000 to 3000 mg 1⁻¹ increased inhibition of shoot growth by flurprimidol, but the opposite was true for paclobutrazol.     

The impact of photoperiod and irradiance on flowering of several herbaceous ornamentals

Forty-one herbaceous species were grown under short-days (8 h photoperiod, ambient irradiance averaged 12–13.2 and 6.4–8.3 mol m⁻² day⁻¹ for Experiments I and II, respectively) with or without supplemental high-pressure sodium lighting (+50, 100, or 150 μmol m⁻² s⁻¹); or under long-days delivered using natural day lengths and irradiance with night interruption lighting (2200–0200 h at 2 μmol m⁻² s⁻¹ from incandescent lamps) or under ambient daylight plus supplemental irradiance during the day and as a day extension to 18 h (0800–0200 h) with supplemental high pressure sodium lighting (+50, 100, or 150 μmol m⁻² s⁻¹) to identify the impact of photoperiod and irradiance on flowering of each species. Days to first open flower, leaf number below first flower, and mean dry weight gain per day (MDWG) were measured when the first flower opened. Twenty-seven species were photoperiodic with examples of five photoperiodic response groups represented: obligate short-day (2), facultative short-day (5), obligate long-day (16), facultative long-day (4); 13 were day neutral (no photoperiod response in flowering). One species, Salvia sclarea L., did not flower. A facultative irradiance response was observed with 10 species; 28 species were irradiance indifferent; 2 had delayed flowering as irradiance increased. Photoperiod affected MDWG of 30 species. Increasing irradiance affected MDWG with 14 species. Photoperiod interacted with irradiance to affect MDWG of 11 species. Cobaea scandens had the greatest MDWG (0.40 g day⁻¹) while Amaranthus hybridus had the least MDWG (0.01 g day⁻¹) across photoperiod and irradiance levels.     

Comparison of growth,leaf water relations and gas exchange of Cistus albidus and C. monspeliensis plants irrigated with water of different NaCl salinity levels

Four-month-old potted Cistus albidus and Cistus monspeliensis plants growing in a greenhouse were submitted to saline stress from 9 August to 2 December, using irrigation water containing 0, 70, and 140 mM NaCl. C. monspeliensis plants are more tolerant to saline irrigation water than C. albidus plants, mainly due to their capacity to resist stress with a lower plant biomass and canopy area; furthermore, they showed no leaf necrosis symptoms. Under saline stress conditions the main growth limiting factor in both species was photosynthesis. Both Cistus species responded to saline stress by developing avoidance and tolerance mechanisms. The avoidance mechanisms took place at a morphological and physiological level. Morphologically, the reduction in the canopy area can be considered a mechanisms for regulating water loss via transpiration. Treated C. monspeliensis plants showed a greater capacity to absorb water and were able to conserve it more efficiently than C. albidus plants. Tolerance mechanisms included Na⁺ and Cl⁻ inclusion and osmotic adjustment. However, the reaction of each species to osmotic adjustment was different, because in C. monspeliensis plants the osmotic adjustment was unable to prevent a decrease in leaf turgor. The curvilinear relationship between P_n and g_l observed in C. monspeliensis plants indicated stomatal limitation of photosynthesis below a leaf conductance of about 160 mmol m⁻² s⁻¹. In C. albidus plants, a linear relationship between photosynthesis and leaf conductance rather a curvilinear model was significant, indicating limitation of the photosynthetic capacity.