Survey of 1975 wheat and soybeans for aflatoxin, zearalenone, and ochratoxin

Wheat samples (102 lots) were collected from Virginia, North Carolina, southeastern Missouri, southern Illinois, and Kentucky. Soybean samples (180 lots) were collected from Virginia, Illinois, Iowa, Minnesota, Nebraska, Alabama, Arkansas, and Texas. Samples of both commodities were analyzed for zearalenone, aflatoxin, and ochratoxin by the Eppley method. None of the 3 mycotoxins was detected in soybeans. Aflatoxins and ochratoxin A were not detected in wheat, but zearalenone was detected in 19 of 42 samples collected in Virginia. Half of the Virginia samples were collected because they were mold-damaged. Zearalenone levels ranged from 0.36 to 11.05 ppm; the identity of the zearalenone was confirmed by gas-liquid chromatography and mass spectroscopy. Gibberella zea infection (6-60%) was detected in all of the zearalenone-positive samples; 6-60% of the kernels in the samples tested contained G. zea.     

Rapid, economical method for determination of aflatoxin and ochratoxin in animal feedstuffs

A quantitative procedure widely used in European Economic Community (EEC) countries has been successfully scaled down to produce a rapid method for determination of aflatoxin B1 (and other aflatoxins) in animal feeds. Without modification, the method may be used for simultaneous ochratoxin A determination in simple feeds, but a slightly different extraction procedure is required for compound feeds. Validity of the method has been demonstrated by comparison with the full EEC procedure for aflatoxin B1 and the Nesheim method for ochratoxin A. Analyses may be completed within 2 h and there is a considerable savings in materials over the 2 reference methods. The procedure is also less hazardous because volumes of toxic extract are small, and the operator is exposed to minimum solvent vapor.     

Screening method for the detection of aflatoxin, ochratoxin, zearalenone, penicillic acid, and citrinin.

A modification of the official method for ochratoxins and a screening method for zearalenone, aflatoxin, and ochratoxin is described and expanded to include citrinin and penicillic acid. The method uses 0.5N phosphoric acidchloroform (1+10) in the initial extraction; the extract is divided and eluted from 2 columns to provide a quantitative thin layer chromatographic (TLC) method for aflatoxin and ochratoxin in corn and dried beans. Aflatoxin and zearalenone are eluted from one column and ochratoxin, penicillic acid, and citrinin from the other. Ochratoxin A recoveries are low (50%) in peanuts. Zearalenone, penicillic acid, and citrinin were qualitatively recovered from corn and beans; zearalenone and penicillic acid were recovered from peanuts but citrinin was not. Several TLC solvents were used to separate interferences.     

A Comprehensive Survey of Soft Wheat Grain Quality in U.S. Germplasm

Wheat (Triticum aestivum L.) quality is dependent upon both genetic and environmental factors, which work in concert to produce specific grain, milling, flour, and baking characteristics. This study surveyed all of the 132 soft wheat varieties (cultivars and advanced breeding lines) grown in the U.S. regional nursery system, which encompassed the three main soft wheat producing regions of the United States (eastern and southern soft red winter and western soft white). The quality parameters included test weight, kernel hardness, weight, and diameter, wheat and flour protein, polyphenol oxidase, break flour yield, flour yield, flour ash, milling score, flour swelling volume, flour SDS sedimentation volume, solvent retention capacity (SRC) for water, sodium carbonate, sucrose, and lactic acid, Rapid Visco Analyzer peak pasting viscosity, and cookie diameter. High levels of variation were observed among varieties, regions, and specific environments, with environment being in general a much greater source of variation than varieties. Variety was observed to have a relatively stronger influence on wheat quality in the western nurseries, compared with the eastern and southern regions, where location effects had a stronger impact on overall wheat quality. The greater influence of variety was particularly notable for kernel hardness in the western nurseries. Kernel hardness also varied considerably as a result of environment. For the two soft red winter wheat nurseries, the western U.S. environment produced substantially harder kernels (37–40) compared with the same varieties grown in eastern U.S. locations (15–20). Intertrait quality relationships were observed to be unique to the specific nursery and germplasm in which they were studied, and these relationships were not consistent across nurseries. Nevertheless, on average, soft wheat quality was fairly similar across the United States, indicating that breeding and testing models have been successful in achieving a relatively uniform target for quality. However, many traits showed high levels of variability among varieties, suggesting that a greater level of selection for end‐use quality would benefit end users by increasing consistency and reducing variability. The often large role of environment (location) in quality indicates that end users must be assiduous in their origination and grain procurement. Clearly, “nursery mean” quality does not reflect the potential that can be obtained, as reflected by a few exceptional soft wheat varieties.     

Reaction to a paper by Whitaker and Dickens on aflatoxin testing plans for shelled peanuts in the U.S. and the export market

The present paper examines a technical paper of Whitaker and Dickens on aflatoxin testing plans that discusses (without a literature reference) a testing plan used in The Netherlands. However, this testing plan has never been in operation. We present the current situation in The Netherlands with respect to legislation and sampling plans on aflatoxin, which has fairly important consequences for the results of the simulation study of Whitaker and Dickens. It is shown that the percentage of rejected U.S.-exported lots in The Netherlands would increase from 16% to 27% based on the actual testing plan in The Netherlands. The need for international harmonization of testing, and the role of Codex Allmentarius is also emphasized.     

Survey for aflatoxin B1 in chicken eggs

Samples of egg products were obtained during January and July 1977 from 35 establishments located in the southern part of the United States. Of the 112 samples analyzed, aflatoxin B1 was found in 1 sample of liquid egg white at a level of 0.06 ng/g. No aflatoxin was found in 101 samples of shell eggs offered for sale to consumers in Alabama, Georgia, South Carolina, and North Carolina in the late fall of 1977.     

Survey of mycotoxins in U.S. distiller's dried grains with solubles from 2009 to 2011

Distiller's dried grains with solubles (DDGS) is a major coproduct of the fuel-ethanol industry and is becoming a popular low-cost ingredient for animal feed. Uncertainties regarding the risk factors in DDGS, such as level of mycotoxins, could limit its application in the animal feed industry. To provide a scientifically sound assessment of the prevalence and levels of mycotoxins in U.S. DDGS, we measured aflatoxins, deoxynivalenol, fumonisins, T-2 toxin, and zearalenone in 67 DDGS samples collected from 8 ethanol plants in the midwestern United States from 2009 to 2011. Among the five mycotoxins, deoxynivalenol was the main focus of the study because the crop year of 2009 was favorable for deoxynivalenol occurrence in corn. We learned that no more than 12% of the samples contained deoxynivalenol levels higher than the minimum advisory level for use in animal feed provided by the U.S. FDA, and the deoxynivalenol levels in all DDGS collected in 2011 were <2 mg/kg. Besides, intensive study showed that the enrichment of deoxynivalenol from contaminated corn to DDGS was about 3.5 times. With regard to the other mycotoxins in DDGS, the study suggested that (1) almost none of the DDGS samples produced in 2010 contained detectable aflatoxins and the highest level of aflatoxins in DDGS was 5.7 μg/kg; (2) no more than 6% of the samples contained fumonisin levels higher than the guidance level for feeding equids and rabbits provided by the U.S. FDA; (3) none of the samples contained T-2 higher than the detection limit; (4) most samples contained zearalenone levels between 100 and 300 μg/kg. This study was based on representative DDGS samples from the U.S. ethanol industry, and the data were collected using state-of-the-art analytical methodology. This study provided a comprehensive and scientifically sound assessment of the occurrence and levels of mycotoxins in DDGS produced from 2009 to early 2011 by the U.S. ethanol industry.     

Scientific principles for water utilization in the U.S.S.R.

Water resources are essential for economic and cultural development of a territory. Both economic development and the health of the people depend on the provision of pure water. Scientifically founded plans of water resources use and conservation should be based on a balance approach to the assessment of available water supply sources and, on the forecast of their interconnected change in the future in a close association with the forecast of the whole economy and culture of the studied region. In the future the main threat to the normal development of the economy and life will not be water deficiency but the conversion of rivers, lakes and other water supply sources into sewers. Therefore it is proposed that there be a complete cessation of sewage discharge (even of the so-called purified) into water supply sources while drawing up long-term water management balances.     

Occurrence of ochratoxin A- and aflatoxin B1-producing fungi in Lebanese grapes and ochratoxin a content in musts and finished wines during 2004

This paper reports the results of an extensive survey on the occurrence of filamentous fungi isolated from wine-grapes in Lebanon and to test their ability to produce ochratoxin A (OTA) and aflatoxin B1 (AFB1) on CYA culture medium, in order to assess their potential for producing these mycotoxins on grapes. From the 470 grapes samples taken during season 2004, 550 fungi strains were isolated with 490 belonging to Aspergillus spp. and 60 belonging to Penicillium spp. All these isolated fungi starins were tested for their ability to produce OTA and AFB1. Aspergillus carbonarius shows that it is the only species able to produce OTA with a production percentage reaching 100% and a maximum concentration of 52.8 microg/g of Czapek yeast extract agar (CYA). In its turn, Aspergillus flavus was considered as the only AFB1-producing species with production percentage of 45.3% and a maximum concentration reaching 40 microg/g CYA. A total of 47 handmade musts produced from the collected grapes were also analyzed in order to correlate the presence of OTA in must and the occurrence of filamentous fungi on grapes; 57.4% were contaminated with OTA at low level with concentrations ranging between 0.011 and 0.221 microg OTA L(-1). The analysis of these must samples was not performed with regard to AFB1. Seventy samples of finish red wine were also assayed for OTA content. The results showed that 42 of the tested samples (60%) were found to be positive for OTA with low levels (0.012-0.126 microg OTA L(-1)).     

Simultaneous thin layer chromatographic determination of aflatoxin B1 and ochratoxin A in black olives

A screening method has been developed for simultaneous determination of aflatoxin B1 and ochratoxin A in black olives. The technique includes extraction of both mycotoxins with aqueous methanol, cleanup using lead acetate, defatting with hexane, partitioning in chloroform, and thin layer chromatography. Detection limits are 5-7 micrograms aflatoxin B1 and 20 micrograms ochratoxin A/kg.     

Rapid thin layer chromatographic method for determining aflatoxin B1, ochratoxin A, and zearalenone in corn

A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1N NaOH is added to separate zearalenone and aflatoxin B1. The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries (%) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.     

Control technology for coal-fired combustion in northeastern U.S.

The status of air pollution control technology for coal fired industrial and power plant boilers has been surveyed. Lime and limestone based scrubbers are capable of removing as much as 93% of flue gas SO₂ at facilities supplying 800 MW power. A sludge containing SO _x ions must be discarded in the process, usually requiring landfill or a settling pond. The Wellman-Lord, thiosorbic, and citrate processes are capable of producing salable products instead of the sludge. They are successful (85 to 90% removal) on demonstration-scale units. The Wellman-Lord process is currently being tested on large-scale coal fired units. Electrostatic precipitators, the mahor control device for removing particulates, can eliminate 99.5% of the fine particles. Fabric filtration can remove 99.9% and is more efficient in the 0.2 to 2μm size range than ESPs. Utilization of fabric filters is expected to increase significantly from its present 5% of the particulate removal market. Nitrogen oxide concentrations in coal fired systems are usually reduced by modification of the combustion system. No significant NO _x reduction on full-scale boilers has yet been reported, but demonstrations are planned over the next few years. Various process capital and annualized costs are reported for SO₂ and particulate removal systems. The status of fluidized bed combustion, fuel desulfurization, conversion of coal to gaseous and liquid fuels, and flue gas denitrification is also discussed. Part A deals with S emission; Part B with NO _x , particulates, and combined systems.     

Anaerobic nitrogen-fixing bacteria in U.S.S.R. soils

An account is given of a study of the size and diversity of populations of Clostridium pasteurianum and C. acetobutylicum in representative soils sampled at various latitudes and altitudes within the U.S.S.R. Evidence is presented to show that the two species are well adapted to soil and climatic factors in terms of the relative dominance of the two species and in terms of differences in their growth-temperature characteristics, carbohydrate utilization and fermentation products, nitrogen fixation and the comparative chemistry of their cells. In all of these characteristics, C. pasteurianum was more variable than C. acetobutylicum.     

Acute and chronic disability among U.S. farmers and pesticide applicators: the National Health Interview Survey (NHIS)

The National Health Interview Survey (NHIS) is a multipurpose household survey of the U.S. civilian non-institutionalized population conducted annually since 1957. From 1986 to 1994, over 450,000 U.S. workers, age 18 years and older, participated in a probability sampling of the entire non-institutionalized U.S. population; variables collected included a range of measures of acute and chronic disability. The objective of the present study was to assess predictors of health status, and acute and chronic disability for farmers and pesticide applicators (pesticide-exposed workers) compared to all other U.S. workers using the 1986-1994 NHIS. After adjustment for sample weights and design effects using SUDAAN, several measures of acute and chronic disability and health status were modeled with multiple logistic regression. Farmers (n = 9576) were significantly older compared to all other U.S. workers (n = 453,219) and pesticide applicators (n = 180). Farmers and pesticide applicators had a higher proportion of males, whites, and Hispanics and were less educated. After adjusting for age, gender, race-ethnicity, and education, compared to all other workers, farmers were significantly less likely to report acute and chronic disability and health conditions, while pesticide applicators were more likely to report chronic disability, health conditions, and poor health. Given the cross-sectional nature of the data and the significant job demands of farming, both leading to a relative healthy worker effect, the present results indicate that at any point in time, farmers report less acute and chronic disability, compared to other U.S. workers, whereas pesticide applicators report similar or poorer health.     

Conservation tillage for soybean in the U.S. Southeastern Coastal Plain

Double cropping of soybean has progressed less rapidly in the U.S. Southeastern Coastal Plains than expected by the ample rainfall and long frost-free season. Post-emergence herbicides, the management of plant residues to reduce water use by cover crops, and a no-till planter with a combination subsoiler are the innovations that have facilitated this new production. Full-season soybean (Glycine max L.) was planted following a grazed cover crop of winter rye (Secale cereale L.) or late-season soybean was planted following winter wheat harvest. In both cases, a special planter was used with an integral subsoil shank ahead of the opener. Full-season soybean under conservation tillage produced yields equal to or better than yields in conventional clean tillage. In a dry summer, soybean yields under conservation tillage exceeded conventional tillage because of suppressed early biomass production which conserved stored soil water and favored growth during the reproduction phase of the crop-cycle. Late-season soybean yields behind wheat favored the conservation tillage practice of in-row subsoil-planting into stubble. However, planting in burned-off wheat stubble produced the highest yields in this study. In a dry spring, the cover crop accelerated soil water use which resulted in lower soybean yields under conservation tillage. Comparisons of 76 vs. 97 cm row spacing were inconclusive, but the trend suggests that wider rows conserve water under periods of drought and that the narrower-row configuration favors adequate water regimes.     

Forest management and biomass in the U.S.A.

There are a variety of opportunities to change land and forest management and, at the same time, create a positive impact on the current use of fossil energy. To the extent that these opportunities can be captured, they address the root cause of greenhouse warming-fossil fuel emissions-while, at the same time, improving economic opportunities, ecosystem productivity, and environmental conditions over broad areas. The need for better markets to absorb biomass energy, plus research to make biomass conversion more efficient, is probably the most important deterrent to achieving these possibilities.     

Using the CERES-Maize model to estimate production for the U.S. Cornbelt

The ability of the CERES-Maize model to estimate annual fluctuations in maize (Zea mays L.) production for the U.S. Cornbelt was tested for the years 1982–1985. The model was run for 51 weather stations in the 14 states of the Cornbelt, which account for 85% of U.S. maize production. The model was calibrated for the region by deriving varietal coefficients for each station based on minimal growth stage data and yields for the 1982 season. Eight sets of varietal coefficients were derived for the 51 stations and should be interpreted as representing all the hybrids grown in an area, rather than a specific hybrid. For 1982 (the calibration year), 1983, 1984, and 1985, model production estimates were 92, 97, 98, and 101%, respectively, of the official U.S. government estimates. The results indicate that the model may be used for large area yield and production estimation in the U.S.A. with minimal regional calibration. The model has the potential for large area yield estimation in other parts of the world where daily maximum and minimum temperatures, precipitation, and solar radiation data are available.     

Sangamon paleosols of southwestern Indiana,U.S.A.

In southwestern Indiana, the Sangamon paleosol transgresses Illinoian glacial drift and weathered bedrock of Pennsylvanian and Mississippian ages in an unglaciated area. The paleosol is buried beneath Wisconsin age loess that thins from 3.2 to 0.5 m in a distance of 83 km eastward from the Wabash River Valley. The buried soil is redder, has more clay in the illuvial horizon, and also has a thicker solum than overlying ground soils formed in loess. Base saturation and pH states of the paleosol relate to secondary enrichment after burial. Free iron-oxide content relates to redder colors and is a genetic property of the buried soils. Greater depletion of illite and chlorite in the paleosol shows that it is more weathered than the overlying ground soil. Pedologic contrasts permit recognition of the Sangamon stage as an event of greater magnitude in the Quaternary than all postloess and Recent time in southwestern Indiana.The Sangamon paleosol has the redder colors and forest-soil profiles on well-aerated, upland, paleogeomorphic sites for a distance of 1000 km to the west. The Sangamon environment probably was uniformly warm, humid forest and woodland not unlike the climatic-vegetation pattern in southern United States today. The present ground soils have more diverse patterns ranging from forest soils in Indiana westward through grassland prairie soils to grassland chernozemic soils of the easternmost Great Plains and with related humid to dry subhumid climates.