南极磷虾粉氨基酸营养价值分析与评价

试验旨在评价南极磷虾粉氨基酸营养价值。采用国标方法,测定南极磷虾粉中氨基酸含量及组成,并将12种水产动物肌肉氨基酸组成作为参考模式,评价南极磷虾粉作为饲料蛋白源的氨基酸营养价值,并与鱼粉和豆粕进行比较。试验结果表明：南极磷虾粉中总氨基酸、必需氨基酸和呈味氨基酸分别占干物质总重的67.18%、32.64%和23.73%,必需氨基酸和呈味氨基酸分别占氨基酸总量的48.59%和35.32%,必需氨基酸和非必需氨基酸的比值为94.50,符合FAO/WHO的理想蛋白质模式。通过氨基酸比值系数法计算,南极磷虾粉第一限制性氨基酸主要为蛋氨酸,而在所选的水产动物中,南极磷虾粉适合作为点篮子鱼、黄颡鱼和红鳍东方鲀的饲料蛋白源。南极磷虾粉氨基酸平衡性较好,营养价值较高,是一种值得开发利用并在水产动物饲料中应用的优质资源。     

Relationship between the Amount of Linoleic Acid in the Diet and That in the Adipose Tissue of Adult Cats Living Freely in Households

The present study was carried out to ascertain whether linoleic acid intake and the content of this fatty acid in adipose tissue are correlated in cats. The linoleic acid intake, as estimated from a food intake questionnaire, was indeed correlated with the linoleic acid content of the abdominal body fat in 165 adult, female cats. A statistically significant linear correlation coefficient of 0.43 was found between the percentage of linoleic acid in the adipose tissue and that in the diet. It is plausible that the observed acid content of adipose tissue of free-living cats may be considered to be a valid biomarker of linoleic acid intake that might be used in studies on diet and chronic disease.     

美仁牦牛肉不同部位常规营养成分及氨基酸和脂肪酸测定分析

摘 要：选取放牧条件下体况良好、体重相近的甘南成年美仁公牦牛 6 头、母牦牛 4头进行屠宰,采取美仁牦牛肉的肩甲、背最长肌、股二头肌三个部位的分割肉。测定不同部位营养成分、氨基酸和脂肪酸。 结果显示：不同部位美仁牦牛肉的蛋白质含量为22.97%～23.07%,其中股二头肌蛋白质含量最低,背最长肌最高;脂肪含量为1.27%～2.49%,股二头肌脂肪脂肪含量较低,不足1.27%。氨基酸含量丰富,脂肪酸总量依次为肩甲、背最长肌、股二头肌上脑肉。通过对比分析不同部位美仁牦牛肉中营养成分、氨基酸和脂肪酸等含量存在差异。     

羔羊日粮精粗比对其肉及脂肪组织中脂肪酸组成的影响

为了研究不同精粗比日粮对广河羊羔肉品质的影响,在广河县羔羊肉生产区,选择不同精粗比饲喂的6月龄健康陶寒杂交(陶赛特羊♂×小尾寒羊♀)断奶去势公羔各3只进行屠宰,取股二头肌、背最长肌、肱二头肌3个部位肌肉和肾周脂肪、皮下脂肪、尾部脂肪3个部位脂肪,用气象色谱法分析其脂肪酸的种类及含量。结果表明,在所有组织中均检测出38种脂肪酸,其中饱和脂肪酸17种,不饱和脂肪酸21种。脂肪组织中饲喂低精粗比日粮的亚油酸(C18∶2n6c)、α-亚麻酸(C18∶3n3)、饱和脂肪酸(SFA)、多不饱和脂肪酸(PUFA)及其与饱和脂肪酸比值(P/S)、n3系多不饱和脂肪酸和n6系多不饱和脂肪酸均显著高于饲喂高精粗比日粮(P<0.05);饲喂低精粗比日粮的所有组织中花生四烯酸(C20∶4)的含量显著高于饲喂高精粗比日粮(P<0.05);与饲喂高精粗比日粮的羊羔肉相比,饲喂低精粗比日粮既节省了生产成本又明显改善和提高了羊羔肉中脂肪酸的营养价值。     

Comparison on the physico‐chemical and nutritional qualities of normal and abnormal colored fresh chicken liver

This study evaluated the differences of physico‐chemical and nutritional qualities between abnormal colored chicken livers (ANCCLs) and normal colored chicken livers (NCCLs) and the safety of the both livers. Compared with NCCLs, ANCCLs were lower in protein, water contents (P < 0.01), pH and pigment contents (P < 0.05). NCCLs contained higher polyunsaturated fatty acid (PUFA) and saturated fatty acids (SFA) (P < 0.05). The PUFA/SFA ratio of NCCLs was 0.453, higher (P < 0.05) than that of ANCCLs. The contents of alanine, valine, tyrosine, lysine and histidine in NCCLs were higher (P < 0.05) than in ANCCLs. The contents of K, Na, P, Cu, Fe and Se of NCCLs were higher (P < 0.05), but the Ca content was lower (P < 0.05). The content of the heavy metals (As, Hg, Pb and Cd) of the two types of livers complied with food safety requirements. Although NCCLs had higher nutritional value than ANCCLs, both livers were acceptable for human consumption.     

Investigations of the maintenance system of the Konik Polski horse and its effects on fecal microbiota activity during the winter and summer seasons

Gastrointestinal microbiota play a key role in the nutrients digestion and hence maintaining animal health and welfare. The diet offered to the animals in captivity may differ considerably from that on natural pastures. In a stabled maintenance system, horses have a limited choice of habitat and feed. Time spend for feeding is relevant for equine welfare because the reduction of the time devoted for foraging may be responsible for inducing gastric inflammation and ulceration. Therefore, in the present study, it was hypothesized that fecal bacterial fermentative processes differ between free-roaming and stabled Konik Polski Horses (KPHs) with respect to microbial enzymatic activity, and thus the fecal concentration of short-chain fatty acids (SCFA), which can be further utilized for assessing the feeding behavior and welfare in free-roaming versus stabled horses. The SCFA concentration and profile, as well as the extracellular and intracellular activities of selected bacterial enzymes, were characterized in horse feces collected during the winter and summer feeding seasons. The results showed higher enzymatic activity and SCFA production in the feces excreted by free-roaming versus stabled horses, especially during summer. An increase in pasture plant diversity may be beneficial for the gastrointestinal microbiota and hence for maintaining health and welfare.     

Effects of the fattening period on the fatty acid composition of fat deposits and free amino acid and inosinic acid contents of the longissimus muscle in carcasses of Japanese Black steers

The effects of the fattening period on carcass characteristics, fatty acid composition of fat deposits, and muscle free amino acid (FAA) and inosinic acid (IMP) contents were evaluated in Japanese Black steers. Ten castrated, 10-month-old calves derived from the same sire were divided into five to be slaughtered at the age of 30 months after a 20-month fattening period (20-month group) and five to be slaughtered at the age of 34 months after a 24-month fattening period (24-month group). Concerning the fatty acid composition of subcutaneous fat, the percentage of palmitoleic acid was higher ( P  < 0.05) in the 24- than in the 20-month group, but no difference was noted in any other fatty acids. For intermuscular fat, no difference was observed in any fatty acids. The percentages of oleic acid and total monounsaturated fatty acid of intramuscular and perinephric fat were higher ( P  < 0.05) in the 24- than in the 20-month group. Of the FAAs in the longissimus thoracis muscle, the threonine and tyrosine contents were lower ( P  < 0.05) in the 24- than in the 20-month group. The IMP content was higher ( P  < 0.05) in the 24- than in the 20-month group, suggesting an effect of prolongation of the fattening period.     

饲料中添加高级脂肪醇对青虾生长性能、机体营养组成和窒息点的影响

试验旨在研究不同添加形式及水平的高级脂肪醇对青虾生长性能、营养组成及窒息点等的影响。挑选尾均重在(0.091±0.016)g的虾苗作为试验用虾,每个水族缸放养100尾,一个水族缸为一个试验组,设计了10组,处理组分别为在基础饲粮中添加0%(T1)、2.5%C28(T2)、5.0%C28(T3)、5.0%C28-PZ(T4)、5.0%C28-PK(T5)、2.5%C30(T6)、5.0%C30(T7)、5.0%C30-PZ(T8)、5.0%C30-PK(T9)、15.0%C30-PZ(T10)脂肪醇。试验结果表明:对照组T1的末重最小,试验组T10末重最大,并显著高于其他试验组。青虾成活率以试验组T1、T3、T5、T6和T9最高,成活率达到75%以上。试验组T1、T2、T4的全虾水分含量较高,试验组T4、T6、T7、T8、T9和T10的粗蛋白含量相对较高,全虾的粗脂肪以试验组T3、T9和T10最高。饲料中添加脂肪醇明显影响青虾的窒息点,添加脂肪醇C28-5可以使青虾的窒息点明显升高。综合青虾生长、成活和窒息点等因素,C28-2.5和C30-PK-5能显著促进青虾生长,降低窒息点,增强抗逆能力,值得作为水产饲料添加剂推广应用。     

Relationships between the contents of vaccenic acid, CLA and n − 3 fatty acids of goat milk and the muscle of their suckling kids

The relationship between goat milk fatty acid composition, especially trans-11 C18:1 (vaccenic acid, VA), conjugated linoleic acid (CLA) and n − 3 fatty acids, and that of the meat from their suckling kids was investigated by feeding the dams a diet supplemented with whole cottonseed (COT) or extruded linseed (LIN). Fifteen Sarda goats, fed 1.2 kg/day per head of concentrate and hay ad libitum, were divided into two groups and supplemented during weeks 4 and 5 post-partum with 32 g/day per head of fat from LIN and COT, which have high C18:3 and C18:2 content, respectively. Fifteen kids were fed exclusively by suckling maternal milk until slaughtering (at 9–10 kg of body weight and approximately 6 weeks of age). Twenty-four hour after slaughter, the longissimus dorsi (LD) muscle was removed from each carcass. Fatty acid profiles of milk and LD were significantly affected by diets. Compared to COT, feeding LIN changed the milk fatty acid profiles by decreasing the proportion of C16:0 and increasing the contents of VA, C18:3 n − 3 (linolenic acid) and total CLA, including cis-9, trans-11-, trans-11, cis-13-, trans-11, trans-13- and trans, trans isomers. The concentrations of VA, cis-9, trans-11 C18:2 (rumenic acid, RA) and linolenic acid in milk and those in kid muscle were strongly correlated. The fatty acid profile of meat from suckling kids was effectively modified by manipulating the diet of the dams.     

Epistatic effects between pairs of the growth hormone secretagogue receptor 1a,growth hormone,growth hormone receptor,non‐SMC condensin I complex,subunit G and stearoyl‐CoA desaturase genes on carcass,price‐related and fatty acid composition traits in Japanese Black cattle

Growth hormone secretagogue receptor 1a (GHSR1a), growth hormone (GH), growth hormone receptor (GHR), non‐SMC condensin I complex, subunit G (NCAPG) and stearoyl‐CoA desaturase (SCD), are known to play important roles in growth and lipid metabolisms. Single and epistatic effects of the five genes on carcass, price‐related and fatty acid (FA) composition traits were analyzed in a commercial Japanese Black cattle population of Ibaraki Prefecture. A total of 650 steers and 116 heifers for carcass and price‐related traits, and 158 steers for FA composition traits were used in this study. Epistatic effects between pairs of the five genes were found in several traits. Alleles showing strain‐specific differences in the five genes had significant single and epistatic effects in some traits. The data suggest that a TG‐repeat polymorphism of the GHSR1a.5′UTR‐(TG)_n locus plays a central role in gene–gene epistatic interaction of FA composition traits in the adipose tissue of Japanese Black cattle.