猪附红细胞体病与猪瘟混合感染的诊治

进入2004年以来，鲁南地区几个县市相继发生了猪附红细胞体病，经过及时确诊和采取有效的治疗基本控制住了疾病的蔓延；除部分断奶前后的仔猪外，整体猪群死亡率在2％以内，对养猪业影响不大。但自从进入秋、冬季以来，病猪死亡率明显增加，个别猪群高达100％，经流行病学调查、病理剖检及实验室检验，确诊为猪附红细胞体病与猪瘟混合感染。经采取综合防制措施，很快控制了疫情。     

猪附红细胞体病继发猪瘟感染的诊治

猪附红细胞体病的发生和流行 ,国内很早就有报道 ,但至今很多地方未能引起重视。我省 2 0 0 1年 6月中旬以来 ,一些地方出现附红细胞体病感染的病猪逾万头 ,死亡率达1 0 %以上 ,损失较大。 2 0 0 1年 8月 ,我市某种猪场也发现大群猪感染附红细胞体病并部分继发感染猪瘟 ,现将此次发病的诊断和治疗情况报告如下。1　发病情况2 0 0 1年 8月 2 7日 ,十堰市某种猪场 1头种公猪发病 ,体温升高达 4 1℃ ,食欲废绝 ,饮水量下降。 3日后 ,1栋种母猪舍开始发病 ,发病头数达 1 6头 ,病猪体温升高至 39 5～ 4 1 5℃。 1周后 ,又 1栋母猪舍 5头母猪发病…     

一起猪弓形体病与附红细胞体病混合感染的诊治

2002年11月中旬,我市某猪场发生以黄疸、高热、呼吸困难、皮肤发红、发绀为主要症状的疾病,现将其发病及诊治情况报道如下。     

猪瘟与附红细胞体病混合感染的诊治

猪瘟（HC）是由猪瘟病毒（Hog cholera virus，HCV）引起的一种烈性传染病，猪瘟又名“烂肠瘟”．是危害养猪业的主要传染病之一。近年来，猪瘟在流行形式、发病特点、临床症状及病理变化等方面都出现了新特点。目前，急性病例及较大规模的暴发式流行极为少见，取而代之的多是病程较长、症状和病变轻微、呈地方性散发流行的“温和型猪瘟”。由于受猪瘟病毒感染的病猪抵抗力降低。所以在气候突变、阴雨潮湿或吸血昆虫繁殖旺盛的季节极易造成与猪附红细胞体病混合感染，若不及时诊治会给养殖户造成严重的经济损失。现将一非典型性猪瘟与猪附红细胞体病？昆合感染的病例报告如下。     

猪瘟与猪附红细胞体病混合感染的诊治

猪瘟又名“烂肠瘟”，是由猪瘟病毒引起的一种严重危害养猪生产的烈性传染病。近年来，猪瘟的流行形式、发病特点、临床症状及病理变化等方面都出现了新特点．急性病程及较大规模的暴发式流行极为少见，取而代之的是病程较长、症状和病变轻微、多地区性散发流行的“温和型猪瘟”。感染猪瘟病毒的病猪抵抗力极低，在气候突变、阴雨潮湿或吸血昆虫繁殖旺盛的季节，极易造成该病和猪附红细胞体病混合感染，若不及时诊治会给养殖户造成严重的经济损失。现将一起典型病例介绍如下。     

附红细胞体病及其混合感染的病例——（一）猪附红细胞体病的诊治

一、发病情况 2005年7月中旬．鲁南某猪场的猪发生附红细胞体病．该猪场哺乳仔猪发病为190头．占发病猪的20％，怀孕母猪发病为59头，占发病猪的14％，育肥猪发病776头．占发病猪的66％。     

猪瘟与猪附红细胞体病混合感染的诊治

1发病情况与临床症状 2004年7月22日，我市某猪场从外地购入100头55日龄的仔猪，8月2日发现有5头猪发病死亡。其临床症状：病猪精神沉郁，食欲减退或拒食，体温升高到40～41．5C，耳尖、颈部、腹部及四肢皮肤有紫红色的出血斑或出血点，压之不褪色，呼吸困难，咳嗽，眼结膜发炎，可视黏膜苍白、黄疸，粪便干燥，带有黏液，排出黄色的尿液。曾用青霉素、安乃近等药物治疗无效。     

猪瘟和附红细胞体并发感染的诊治

我地区沈家台乡一养猪户于今年5月14日从本地购进40多头30日龄杜洛克仔猪，买进后第3d猪群中发现有一头猪发病，临床表一发热，咳嗽、喘、不吃食。经当地兽医诊治后，略有好转，但停药后猪群中又陆续出现病猪，且体温均在40℃以上，有的猪体温高达42℃以上，用头孢西林、先锋、安痛定等药物治疗不见好转，波及全群猪并出现5头仔猪死亡，于6月25日来我们诊求诊     

Dot─ELISA检测规模化猪场猪瘟抗体水平

应用斑点酶联免疫吸附试验（Dot-ELISA)对四川某规模化猪场的43份免疫母猪的血清进行猪瘟病毒抗体水平检测。试验结果：按抗体水平1：80及其以上计为阳性,则阳性血清占30头份,阳性率为69.8%,其中,1：80占14头份（32.6%),1：160占10头份（23.2%),1：320占6头份（14%）;抗体水平在1：40以下占4头份（9.3%）,1：40占9头份（20.9%)。研究表明,Dot-Elisa是一种操作简便,准确,快速的检测方法,特别适应规模化猪场猪瘟抗体水平的监测。     

The hog cholera virus.

Hog cholera virus (HCV) is a spherical enveloped particle of about 40-60 nm dia. The viral genome is a single strand RNA of about 12,000 bases with positive polarity. One single large open reading frame codes for presumably four structural, i.e. three glycoproteins and a core protein, and about three to five nonstructural proteins. The functional role is not yet fully clear for all viral proteins. HCV belongs to the pestivirus group and it is closely related to bovine viral diarrhoea and border disease viruses. The relationship extends to morphology, antigenicity, host spectrum and molecular properties. Pestiviruses hold generic status in the family Flaviviridae.     

Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

Transmission of hog hog cholera virus by mosquitoes.

Mosquitoes trapped during an epizootic of hog cholera (HC) in Maryland in 1969 were prepared into 40 pools which were inoculated in pigs. Hog cholera virus was confirmed in pigs inoculated with 8 of 40 pools of mosquitoes. Generally, the pigs contracting HC developed chronic infections with persistent viremia that lasted 30 or more days. Two pigs seemed healthy when euthatized 62 and 80 days after inoculation, yet viremia of high titer was detected in each. Experimental studies were performed with 2 laboratory strains of mosquitoes, Aedes aegypti and Culex tarsalis, to determine if biological and mechanical transmission occur. Biological transmission was not confirmed, but HC virus was retained in A aegypti for 3 days. Mechanical transmission was confirmed with A aegypti in 2 of 9 experiments.     

狐狸疑似犬瘟热病毒和附红细胞体混合感染的诊治

2006年9月,山东省临沂市某狐场对100只3月龄的幼狐进行犬瘟热灭活疫苗免疫,免疫后第二天30只幼狐突然发病,病狐出现咳嗽、呼吸困难、发热、眼鼻出现卡他性炎症等现象,同时出现贫血、黄疸等症状.     

Effect of dexamethasone on the multiplication of attenuated strains of hog cholera virus in pigs

The spread and multiplication of three attenuated strains of hog cholera (HC) virus was investigated in pigs immunosuppressed with a synthetic corticosteroid (dexamethasone). The distribution and degree of multiplication of the ROVAC lapinized strain of HC virus was enhanced in immunosuppressed pigs in comparison to results in untreated pigs, whilst no differences were noted for the Chinese lapinized strain of HC virus, and only slight variations using the tissue culture attenuated GPE strain.The suggestion is made that the results indicate a higher degree of attenuation in the Chinese and GPE strains of HC virus in comparison with the ROVAC strain.