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由驽巴贝虫和马巴贝虫引起中国北方地区的马焦虫病的流行情况仍然不清楚,在本研究中我们调查了青海省乌兰地区马焦虫病的流行情况。从乌兰县的铜普地区的牧户中收集了97份马血清样品,并分别用重组P48蛋白作为ELISA诊断抗原,进行马巴贝斯原虫的诊断检查,调查青海省乌兰地区马焦虫病的流行情况。1材料和方法1.1通过载体pGEX大肠杆菌表达的P48作为ELISA诊断抗原来诊断驽巴贝虫(Babesia caballi),方法简述如下:重组P48蛋白用谷胱苷肽琼脂糖4B球珠纯化,作为ELISA诊断抗原,检测巴贝虫抗体。巴贝虫阴、阳性标准血清,均为日本国家原虫中心… 相似文献
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米娜瓦尔·阿不都热西木 《吉林畜牧兽医》2021,42(2)
马巴贝氏焦虫和驽巴贝氏虫是导致马属动物巴贝斯虫病的主要病原体,其主要寄生于马属动物的红细胞内并引起血液原虫病,致死率较高,严重危害着马属动物的生长健康以及养殖户的经济效益. 相似文献
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马类巴贝斯虫病主要侵害马、骡、驴。病原是由驽巴贝氏虫(旧名马焦虫)和马巴贝氏焦虫寄生于马属动物的红细胞内所引起的血液原虫病。临床表现发病急、发热、黄疸、贫血、出血和呼吸困难等症状。 相似文献
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广州及其周边地区马梨形虫病血清流行病学调查 总被引:2,自引:0,他引:2
为广东从化无规定马属动物疫病区建设的顺利进行和了解周边地区马属动物马梨形虫病的存在情况,采用酶联免疫吸附试验(ELISA)和涂片镜检的方法对广州、东莞和深圳的马属动物进行了马梨形虫病(马巴贝斯虫和驽巴贝斯虫两种虫体)的流行病学调查。结果表明,上述三个地区的马属动物均存在不同程度的马梨形虫病两种虫体的抗体,且出现同一个马场和在同一匹马同时存在两种虫体抗体的情况。对所有抗体阳性马匹进行涂片镜检未发现虫体,也未发现有马梨形虫病临床症状的马属动物。 相似文献
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马巴贝斯虫病微量补体结合试验的改良 总被引:4,自引:0,他引:4
马巴贝斯虫病(旧称纳氏焦虫病)是由带虫感染蜱传播的,由寄生于马属动物(马、驴、骡等)红细胞内的马巴贝斯虫引起的一种血液原虫病,主要表现为急性、亚急性或慢性发病过程.病马逐渐消瘦、贫血,有的出现黄疸、血红蛋白尿等症状,严重发病者可致死.本病广泛分布于世界各地,我国新疆、内蒙古及南方各省都曾有该病散发或地方性流行. 相似文献
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马驽巴贝斯虫(Babesia caballi)病是马属动物的一种血液原虫病,被农业农村部列为二类动物疫病.本研究旨在建立一种针对马驽巴贝斯虫病的ddPCR检测技术.利用马驽巴贝斯虫特异性引物及探针对反应体系进行优化,测试了方法的特异性、稳定性及灵敏性,对30份可疑样品进行了初步检测.结果显示,该方法具有较好的稳定性,最... 相似文献
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Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China 总被引:5,自引:0,他引:5
Xu Y Zhang S Huang X Bayin C Xuan X Igarashi I Fujisaki K Kabeya H Maruyama S Mikami T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1015-1017
The prevalence of equine piroplasmosis caused by Babesia equi and Babesia caballi in northeast China has remained unknown, although the People's Republic of China is recognized as an endemic country for the diseases. In the present study, we investigated the prevalence of equine piroplasmosis in Jilin province, a part of northeast China. A total of 111 serum samples were taken from horses in eastern Jilin, and examined for diagnosis of B. equi and B. caballi infections by the enzyme-linked immunosorbent assays with recombinant antigens, equi merozoite antigen-1 and P48, respectively. Of the 111 samples, 38 (34%) and 36 (32%) samples were sero-positive for B. equi infection and B. caballi infection, respectively. In addition, 14 (12%) samples were sero-positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in northeast China. 相似文献
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Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens 总被引:1,自引:0,他引:1
Xuan X Chahan B Huang X Yokoyama N Makala LH Igarashi I Fujisaki K Maruyama S Sakai T Mikami T 《Veterinary parasitology》2002,108(2):179-182
The prevalence of equine piroplasmosis in Xinjiang province, China, was examined by enzyme-linked immunosorbent assays (ELISAs). A total of 70 serum samples were taken from horses pastured on three farms in western Xinjiang, and examined for diagnosis of equine Babesia equi (B. equi) infection and B. caballi infection by ELISAs using recombinant equi merozoite antigen 1 (EMA-1) and recombinant P48 antigen, respectively. Of the 70 samples, 28 (40.0%) and 17 (24.3%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 11 (15.7%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in western Xinjiang. To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China. 相似文献
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马梨形虫病是由马驽巴贝斯虫和马泰勒虫寄生于马属动物的红细胞内所引起的一类血液原虫病,呈全球性分布,尤其在新疆发病率更高,处于逐年上升趋势,对区域性马产业的发展影响极大。为了解2018年新疆昭苏养马区域马梨形虫的感染情况,随机采集昭苏县18个乡镇的马全血及血清各858份,采用PCR和间接ELISA分别进行检测,对两种方法检测的18个地区、不同年龄阶段的马驽巴贝斯虫、马泰勒虫及混合感染情况进行统计学分析。结果显示,PCR检测马驽巴贝斯虫、马泰勒虫及混合感染的阳性率分别为12.12%、13.87%和2.80%;间接ELISA检测马驽巴贝斯虫、马泰勒虫及混合感染的抗体阳性率分别为15.50%、10.14%和2.56%;不同年龄阶段筛查结果显示,在6岁以下的马匹感染马驽巴贝斯虫、马泰勒虫及混合感染的阳性率较高,并且不同地区的不同年龄阶段马匹的马梨形虫感染率存在不同程度的差异。此次获得的昭苏县马梨形虫感染情况的一线数据,可为当地养马区域马梨形虫病的综合防控提供技术支撑。 相似文献
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Boldbaatar D Xuan X Battsetseg B Igarashi I Battur B Batsukh Z Bayambaa B Fujisaki K 《Veterinary parasitology》2005,127(1):29-32
The purpose of this study was to demonstrate the occurrence of equine piroplasmosis in Mongolia, a country in which the disease occurs epidemically in different climatic conditions. Antibodies to Babesia equi and B. caballi were determined in serum samples of 254 pastured horses in different locations of Mongolia using an enzyme-linked immunosorbent assay with recombinant antigens. One hundred and eighty-five (72.8%) and 102 (40.1%) of all serum samples were positive for B. equi and B. caballi infections, respectively. In addition, 78 (30.7%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread in Mongolia. To our knowledge, this is the first report describing an epidemiological study on equine piroplasmosis in different geographic regions in Mongolia. 相似文献
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Chahan B Zhang S Seo JY Nakamura C Zhang G Bannai H Jian Z Inokuma H Tuchiya K Sato Y Kabeya H Maruyama S Mikami T Xuan X 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):753-755
The prevalence of Babesia (Theileria) equi and B. caballi infections in donkeys in western Xinjiang China was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashi and Ili areas, and examined for B. equi and B. caballi infections by enzyme-linked immunosorbent assays using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang. 相似文献
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Mujica FF Perrone T Forlano M Coronado A Meléndez RD Barrios N Alvarez R Granda F 《Veterinary parasitology》2011,178(1-2):180-183
The main objective of this study was to demonstrate the occurrence of equine piroplasmosis (EP) in horses of Lara State, Venezuela, and to correlate it with the factors host's sex and age in order to know the epidemiology of this disease at the Venezuelan Centroccidental Region. Antibody levels to Babesia caballi and Theileria equi were assessed in 360 equine serum samples, collected from 9 municipalities of Lara State, using an ELISA technique with recombinant antigens and monoclonal antibodies (Mabs). Antibodies to B. caballi were found in 254 horses (70.6%), whereas 181 animals (50.3%) were detected as seropositives to T. equi. In addition, 128 samples (35.56%) were seropositives to both hemoparasites. There were no significant differences between the seropositivity to B. caballi and T. equi with the factors sex and age of the horses. These results show that Lara State is an enzootic area for equine piroplasmosis, and are a contribution to a partial knowledge of the dynamic of this disease in Venezuela. 相似文献
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C E Kerber F Ferreira M C Pereira 《The Onderstepoort journal of veterinary research》1999,66(2):123-127
The importance of equine piroplasmosis control in endemic countries has increased in recent years and plays an important role to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Babesia equi or Theileria equi and Babesia caballi) in Brazil--a country where the disease occurs endemically--in different climatic conditions, and to evaluate the results of a strategy for tick control in order to decrease infection rates. Blood samples were taken from 720 horses on 28 farms from different regions and subjected for complement fixation testing. The strategy was based on the control of the tick population by spraying the horses with acaricides, treating positive horses and preventing cattle and horses from grazing together. A significant association was found in the prevalence of antibody titres in tropical and subtropical areas. A significantly lower prevalence rate occurred on those farms where measures to control tick population were established. Farms in endemic countries may significantly reduce the prevalence of equine piroplasmosis by establishing measures to control the tick population and treating chronic carrier horses. Additional measures for controlling ticks in tropical areas are also discussed. 相似文献
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Huang X Xuan X Yokoyama N Katayama Y Anzai T Igarashi I 《Veterinary parasitology》2006,140(1-2):158-161
Two enzyme-linked immunosorbent assays (ELISA) with recombinant protein as antigens were evaluated by comparison with the indirect fluorescent antibody tests (IFAT) for the detection of specific antibodies to Babesia caballi and Babesia equi, respectively in 380 sera from experimentally infected, uninfected, and field horses. The high concordances of 92.4% (351/380) and 98.2% (373/380) between ELISA and IFAT for B. caballi and B. equi, respectively suggest that ELISA, especially for B. equi infection, could be alternative to the corresponding IFAT for serodiagnoses of equine piroplasmosis, although some improvements are required in ELISA for B. caballi. 相似文献
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Blood and serum samples were taken from 481 horses, from a stud farm or a racecourse, and tested by microscopic examination of blood smears and cELISA for Theileria equi (T. equi) and Babesia caballi (B. caballi) infections. At the time of sampling, animals were also examined for tick infestations and clinical disease, which were not observed in any of the sampled horses. During the microscopic examination of thin blood smears, parasites were detected in the three horses from the racecourse. Overall seroprevalence of infection was detected as 18.50% (89 of 481 horses) by cELISA, with T. equi being significantly more prevalent than B. caballi. Of the 481 blood samples, 78 (16.21%) were serologically positive for T. equi and 4 (0.83%) were serologically positive for B. caballi. In addition, 7 (1.46%) samples were positive for both T. equi and B. caballi antibodies. Seropositivity rates in the racecourse horses were higher than those determined in the stud farm horses. The rates for T. equi, B. caballi and both species were 13.39, 0.52 and 0% in the horses from the stud farm and 27, 2 and 7% in the racecourse horses, respectively. These results indicate that equine piroplasmosis is more common in racehorses than studhorses and therefore it might be a serious concern in horses that participate to international races. 相似文献
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