仔猪腹泻阴沟肠杆菌的分离及鉴定

温州郊区某专业户饲养母猪45头,2003年11月底曾出现3头母猪死胎和弱胎现象.12月共有8～20日龄存栏小猪60多头,其中有40头发病,主要表现为腹泻;死亡10头.2004年1月,新出生仔猪6窝,其中出现5窝是整窝腹泻,另外1窝12头仔猪中有8头腹泻.先后使用林可霉素、先锋霉素V、土霉素、头孢拉定、诺氟沙星、磺胺嘧啶、杀利王等药物治疗无效.注射阿莫西林和地塞米松混合合液后症状减轻,但仍未控制本病.该场母猪在产前30d均注射过大肠杆菌苗和蓝耳病弱毒苗.     

仔猪黄痢的诊断和预防

贵州省黔西县民远养殖场在 1 983年引进大约克公猪和荣昌母猪进行杂交 ,于同年 1 0月份相继产仔 ,到 1 999年已产仔 1 0 0 0多头。其中发生新生仔猪腹泻病症的约为 30 0多头 ,发病率为 30 %。该场曾用仔猪大肠杆菌基因工程苗进行预防 ,但仔猪生后仍有发病。对发病仔猪曾用痢特灵、氯霉素、红霉素、土霉素等抗生素进行治疗 ,但效果不佳 ,仍有1 2 0头死亡 ,病死率高达 40 %。本文对新生仔猪腹泻病例进行了病原的分离与鉴定 ,并取致病菌株 ,制成活菌苗 ,喂服母猪 ,有效地控制了该场新生仔猪腹泻的发生。报告如下。1　流行病学及临床症状该病主…     

浅谈腹泻保育仔猪的综合护理

在养猪生产中，腹泻较为常见，也易造成重大经济损失。保育仔猪发生腹泻后，若治疗、护理不当，可能迁延病情，影响仔猪的生长性能。采取合理、积极的综合护理措施，可缩短治疗时间，提高仔猪成活率。本文对保温、限饲、补液、合理给药、环境消毒隔离、康复期合理饲喂等6个方面的护理措施进行了总结，供广大养殖业者参考。     

一起猪瘟、附红细胞体和圆环病毒混合感染的病例介绍

1基本情况 2009年1月24日，临汾市某养猪场的猪只出现发热、皮肤出血、下痢等症状，其后出现死亡，使用磺胺药和青、链霉索等药物治疗，均不能控制病情。该养猪场饲养母猪23头，仔猪和育肥猪152头。经调查该猪场曾经发生过温和性猪瘟、附红细胞体、仔猪红、白痢等多种疾病，但死亡率没有此次高，发病前由于大风降温，该场猪舍部分侧棚被刮掉，猪群曾表现出流行性感冒症状。     

新生仔猪丁型冠状病毒腹泻病例诊断报告

2020年7月,南昌某猪场产后3～4日龄的新生仔猪发生腹泻,一直按仔猪流行性腹泻处理,先后接种了防控仔猪流行性腹泻的活疫苗和灭活疫苗各2次,但新生仔猪腹泻未见有所减少.2021年2月,畜主采集2头新生仔猪腹泻病料,至南昌德尔塔生物技术有限公司检测.结果显示,丁型冠状病毒(PDCoV)均呈阳性,而猪流行性腹泻病毒(PEDV)均呈阴性,故确定该场新生仔猪腹泻由PDCoV引起.     

仔猪伪狂犬病合并弓形体感染的诊治

2004年9月份,驻马店市南郊某养殖场的一圈猪舍内仔猪突然发病,主要表现出神经症状和体温升高,该场兽医人员立即采用抗菌药物进行治疗,病情未见好转,随来我处就诊,现报告如下:1发病情况该场于9月6日自正阳县调入断奶仔猪100头,运回第2天在一个圈舍内就有一头仔猪发病并于当天死亡。接着病情开始蔓延,2周内共有70头仔猪发病,死亡48头,死亡率高达68%。发病仔猪多见于体质瘦弱的仔猪。2临床症状潜伏期很短,一般只有1～2天。病初仔猪主要表现精神沉郁,食欲不振,呼吸加快,体温升高,一般在41℃左右。个别仔猪有呕吐和腹泻现象,口角有大量泡沫或流出…     

日粮应激引起仔猪腹泻的诊断和治疗

某养殖户饲养了60头15kg左右的仔猪。2005年4月14日上午,饲料更换为营养更全面的日粮。饲喂后,仔猪陆续发生了以腹泻为主要症状的疾病,至4月15日下午,全群仔猪均发生了腹泻。该场有良好的卫生和防疫条件。此前,仔猪的生长发育一直良好,无其他疾病。仔猪发病后,畜主用     

污水引起仔猪中毒性腹泻的诊断报告

一、病情调查(一)发病情况:1984年5—6月,我省某猪场暴发一起原因不明的仔猪中毒性腹泻。该猪场共有250头猪,其中断奶仔猪及哺乳仔猪共62头。从5月中旬开始,全部仔猪均在短期内出现腹泻,药物治疗效果不     

立泻止对仔猪腹泻的预防效果

将3日龄仔猪随机分为3组,即高剂量试验组、低剂量试验组、抗生素对照组。用注射器将配好的立泻止注入口腔内。7日龄时和断奶时再用相同的剂量投药。对照组的仔猪发病后即用抗生素注射治疗按说明书要求给药,现配现用。投药后每天观察,分别记录腹泻、发病、死亡情况。第一次投药前、第二次投药时、断奶时和断奶后15天时对每头仔猪进行称重,统计仔猪的增重情况。结果表明:高剂量组17.30%仔猪发生腹泻,其中1头仔猪因腹泻死亡;低剂量组有23.50%发生腹泻,其中1头仔猪因腹泻死亡,还有1头仔猪因其它原因(不能站立)死亡;对照组有30头仔猪(30/56,53.60%)发生腹泻,其中2头仔猪因腹泻死亡,还有1头仔猪因其它原因(呼吸困难)死亡。高剂量组的每头试验仔猪平均增重为9220g,低剂量组的每头试验仔猪平均增重为9450g,对照组的每头仔猪平均增重为9280g。从结果上看,高剂量组的增重情况不如对照组,而低剂量组的增重情况明显好于对照组,但差异不显著。立泻止能有效预防仔猪腹泻,并且有一定的增重效果。     

仔猪蛔虫 鞭虫混合感染的诊治

1发病情况发病猪场为新建场,采用自繁自养方式用发酵床养猪,猪场共饲喂母猪22头、40～80日龄仔猪82头。由于建场不久,饲养管理经验缺乏,猪场从未给猪只驱过虫。2011年9月中旬,部分仔猪出现腹泻症状,经抗菌消炎、止泻、抗病毒等治疗,效果不明显。82头仔猪中,有腹泻症状的有36头,发病率43.9%,猪只     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.