Interference between red kidneybean (Phaseolus vulgaris L.) cultivars and redroot pigweed (Amaranthus retroflexus L.)

Field experiments were conducted in 2006 and 2007 to evaluate the competitive ability of bush type red kidneybean (RKB) (Phaseolus vulgaris L.) cultivars against redroot pigweed (Amaranthus retroflexus L.). Three cultivars of RKB (Akhtar, Sayyad and D81083) and five A. retroflexus densities (0, 4, 8, 16 and 32 plants m⁻²) were established in a factorial arrangement. A. retroflexus had a greater plant height and growth rate (GR) but a lower leaf area index (LAI) than RKB cultivars in almost all treatments. Higher densities of A. retroflexus increased LAI and GR but decreased yield of RKB cultivars. The cv. Sayyad and D81083 had the greatest and lowest LAI and GR, respectively, in competition with A. retroflexus. The maximum intercepted photosynthetically active radiation (PAR) at noon by A. retroflexus was 90.4 and 66.0% in competition with cv. D81083 and Sayyad, respectively. The seed yield and pod number per plant of RKB cultivars decreased severely with increasing A. retroflexus density. A. retroflexus seed number m⁻² was the highest and lowest in competition with cv. D81083 and Sayyad, respectively. The competitive ability of RKB cultivars was compared using parameters estimated through two-parameter yield loss-relative leaf area model. The relative ranking of the RKB cultivars examined for their competitiveness, supported by modeling results, was Sayyad > Akhtar > D81083.     

Response of giant reed (Arundo donax L.) to nitrogen fertilization and soil water availability in semi-arid Mediterranean environment

The aim of the present work was to evaluate the effect of soil water availability and nitrogen fertilization on yield, water use efficiency and agronomic nitrogen use efficiency of giant reed (Arundo donax L.) over four-year field experiment.After the year of establishment, three levels for each factor were studied in the following three years: I₀ (irrigation only during the year of establishment), I₁ (50% ETm restitution) and I₂ (100% ETm restitution); N₀ (0 kg N ha⁻¹), N₁ (60 kg N ha⁻¹) and N₂ (120 kg N ha⁻¹).Irrigation and nitrogen effects resulted significant for stem height and leaf area index (LAI) before senescence, while no differences were observed for stem density and LAI at harvest.Aboveground biomass dry matter (DM) yield increased following the year of establishment in all irrigation and N fertilization treatments. It was always the highest in I₂N₂ (18.3, 28.8 and 28.9 t DM ha⁻¹ at second, third and fourth year growing season, respectively). The lowest values were observed in I₀N₀ (11.0, 13.4 and 12.9 t DM ha⁻¹, respectively).Water use efficiency (WUE) was significantly higher in the most stressed irrigation treatment (I₀), decreasing in the intermediate (I₁) and further in the highest irrigation treatment (I₂). N fertilization lead to greater values of WUE in all irrigation treatment.The effect of N fertilization on agronomic nitrogen use efficiency (NUE) was significant only at the first and second growing season.Giant reed was able to uptake water at 160–180 cm soil depth when irrigation was applied, while up to 140–160 cm under water stress condition.Giant reed appeared to be particularly suited to semi-arid Mediterranean environments, showing high yields even in absence of agro-input supply.     

Effect of plant density and nitrogen rates upon the leaf area of seed sugar beet on seed yield and quality

Three-year field trials were set up on eutric brown soil in northwestern Croatia (Zagreb) with the objective to determine the effect of plant density and nitrogen rates on the formation and size of leaf area of seed sugar beet, and on the yield and seed quality in seed production without transplanting. Investigations should also reveal how much the yield and quality of sugar beet seed depend on the leaf area index (LAI). Four plant densities of seed sugar beet were investigated after crop wintering (40 000, 80 000, 120 000, and 160 000 plants/ha) as well as three nitrogen rates (60, 120, and 180 kg/ha) applied in two identical topdressings: at the beginning of the spring growing period and immediately before shooting of inflorescence stalks. Leaf area formation was strongly influenced by weather conditions. An increase of plant density from 40 000 to 160 000 plants/ha led to a decrease of leaf area per plant. Raised nitrogen rates in topdressing caused an increase of leaf area, depending on the precipitation and soil fertility. Maximum LAI, achieved in the flowering stage, grew almost linearly with increasing plant density (LAI: 1.77–4.85 m²/m²), but was statistically significant only up to 120 000 plants/ha. Raised nitrogen rates in topdressing led to a significant increase of the LAI in the stage of inflorescence stalk shooting, though not in full flowering. On the basis of this research, seed yield and germination of seed sugar beet could not be predicted regarding LAI in the flowering stage.     

Yield,water use and radiation use efficiencies of kenaf (Hibiscus cannabinus L.) under reduced water and nitrogen soil availability in a semi-arid Mediterranean area

Kenaf is a warm-season species that recently has been proved to be a good source of biomass for cellulose pulp for the paper industry in Mediterranean countries, where the use of hemp is problematic for legal reasons. A two-year research program aiming at studying the effects of different water regimes and nitrogen fertilization levels, upon plant growth, leaf area index, biomass accumulation, water and radiation use efficiency, was carried out on kenaf under a typically semi-arid Mediterranean climate of South Italy. In cv. Tainung 2, four different water regimes (I₀ = no irrigation, I₂₅, I₅₀ and I₁₀₀ = 25, 50 and 100% ETc restoration, respectively) and three nitrogen levels (N₀ = no nitrogen, N₇₅ and N₁₅₀ = 75 and 150 kg ha⁻¹ of N, respectively) were studied. The amount of water applied strongly affected plant growth (in terms of LAI, plant height and biomass) and final total and stem dry yield, which significantly increased from I₀ to I₁₀₀. Nitrogen did not exert any beneficial effect upon dry yield. Radiation Use Efficiency (RUE), calculated in the second year only, was the highest (1.95 g DM MJ⁻¹) in fully irrigated treatment (I₁₀₀) and the lowest (0.86 g DM MJ⁻¹) in the dry control.Water use efficiency (WUE) was rather similar among water regimes, whilst irrigation water use efficiency (IWUE) progressively increased with the decrease of total volume of water distributed to the crop by irrigation, from 3.47 to 12.45 kg m⁻³ in 2004 and from 4.27 to 7.72 kg m⁻³ in 2005. The results obtained from this research demonstrate that in semi-arid areas of South Italy, irrigation at a reduced rate (50% ETc restoration) may be advantageous, since it allowed a 42–45% irrigation water saving, when compared to the fully irrigation treatment, against a 23% (in 2004) and 36% (in 2005) yield reduction, and a still good efficiency (near that potential) in transforming the solar radiation in dry biomass was maintained (RUE = 1.76 g DM MJ⁻¹, against 1.95 g DM MJ⁻¹ in fully irrigated treatment).     

Performance and application of the APSIM Nwheat model in the Netherlands

APSIM Nwheat is a crop system simulation model, consisting of modules that incorporate aspects of soil water, nitrogen (N), crop residues, and crop growth and development. The model was applied to simulate above- and below-ground growth, grain yield, water and N uptake, and soil water and soil N of wheat crops in the Netherlands. Model outputs were compared with detailed measurements of field experiments from three locations with two different soil types. The experiments covered two seasons and a range of N-fertiliser applications. The overall APSIM Nwheat model simulations of soil mineral N, N uptake, shoot growth, phenology, kernels m⁻², specific grain weight and grain N were acceptable. Grain yields (dry weight) and grain protein concentrations were well simulated with a root mean square deviation (RMSD) of 0.8 t ha⁻¹ and 1.6 protein%, respectively. Additionally, the model simulations were compared with grain yields from a long-term winter wheat experiment with different N applications, two additional N experiments and regional grain yield records. The model reproduced the general effects of N treatments on yields. Simulations showed a good consistency with the higher yields of the long-term experiment, but overpredicted the lower yields. Simulations and earlier regional yields differed, but they showed uniformity for the last decade.In a simulation experiment, the APSIM Nwheat model was used with historical weather data to study the relationship between rate and timing of N fertiliser and grain yield, grain protein and soil residual N. A median grain yield of 4.5 t ha⁻¹ was achieved without applying fertiliser, utilising mineral soil N from previous seasons, from mineralisation and N deposition. Application of N fertiliser in February to increase soil mineral N to 140 kg N ha⁻¹ improved the median yield to 7.8 t ha⁻¹ but had little effect on grain protein concentration with a range of 8–10%. Nitrogen applications at tillering and the beginning of stem elongation further increased grain yield and in particular grain protein, but did not affect soil residual N, except in a year with low rainfall during stem elongation. A late N application at flag leaf stage increased grain protein content by several per cent. This increase had only a small effect on grain yield and did not increase soil residual N with up to 40 kg N ha⁻¹ applied, except when N uptake was limited by low rainfall in the period after the flag leaf stage. The economic and environmental optima in winter wheat were identified with up to 140 kg N ha⁻¹ in February, 90 kg N ha⁻¹ between tillering and beginning of stem elongation and 40 kg N ha⁻¹ at flag leaf stage resulting in a median of 8.5 t ha⁻¹ grain yield, 14.0% grain protein and 13 kg N ha⁻¹ soil residual N after the harvest. The maximum simulated yield with maximum N input from two locations in the Netherlands was 9.9 t ha⁻¹.     

In field non-invasive sensing of the nitrogen status in hybrid bermudagrass (Cynodon dactylon × C. transvaalensis Burtt Davy) by a fluorescence-based method

The level of N fertilization and the content of leaf N in Cynodon dactylon × C. transvaalensis Burtt Davy cv. ‘Tifway 419’ bermudagrass were evaluated non-destructively with a fluorescence-based method. It was applied directly into the field by using the Multiplex portable fluorimeter during two consecutive seasons (2010 and 2011). In the 2010 experiment, the nitrogen balance index (NBI₁) provided by the sensor was able to discriminate (at P < 0.05) six different N levels applied, up to 250 kg ha⁻¹, with a precision (root mean square error, RMSE) in the rate estimate of 3.29 kg ha⁻¹. In 2011, the index was insensitive to the N treatment between 150 kg ha⁻¹ and 250 kg ha⁻¹ N rates, and its precision was 39.98 kg ha⁻¹. Calibration of the sensor by using the destructive analysis of turf samplings showed a good linear regression between NBI₁ and the leaf N content for both 2010 (R² = 0.81) and 2011 (R² = 0.93) experiments. This allowed mapping of the leaf N spatial distribution acquired by the sensor in the field with a prediction error of 0.21%. Averaging the overall estimates of leaf N content per N treatment provided an upper limit of 200 kg ha⁻¹ for the required fertilization, corresponding to a critical level of leaf N of about 2.3%. Our results confirm the usefulness of the new fluorescence-based method and sensor for a precise management of fertilization in turfgrass.     

Pig slurry fertilization in a double-annual cropping forage system under sub-humid Mediterranean conditions

In areas of Southern Europe with very intensive pig production, most of the pig slurry (PS) is applied as fertilizer. However, in the European Union, no more than 170 kg N ha⁻¹ year⁻¹ can be applied in nitrate vulnerable zones (NVZs) from livestock manures. In this context, a six-year trial was conducted for a maize-triticale double-annual forage cropping rotation under rainfed conditions. Four different N rates were applied (0, 170, 250 and 330 kg N ha⁻¹ year⁻¹), to evaluate their effect on crop yield, N uptake, unrecovered N and soil nitrate content. The corresponding PS rates were defined as zero (PS 0), low (PSL) medium (PSM) and high (PSH). The annual average dry matter (DM) yields (maize + triticale) for the PS fertilization treatments PS0, PSL, PSM and PSH were 12.6, 17.7, 20.2 and 22.0 Mg DM ha⁻¹, respectively. Maize DM yield was influenced mainly by weather conditions, and triticale DM yield was clearly influenced by initial soil NO₃⁻-N and PS fertilization rates. Unrecovered N was affected by PS fertilization rate and initial soil NO₃⁻-N content. A residual effect of the PS when applied to maize had an important effect on soil NO₃⁻-N and subsequent triticale DM yield. Moreover, total annual average unrecovered N, considering the sum of both crops (maize + triticale), were 91, 144, and 222 kg N ha⁻¹ in PSL, PSM and PSH, respectively. In order to avoid part of this unrecovered N, mainly by lixiviation of nitrates, PS fertilization in triticale should be applied as side dressing at tillering. The application of N, in the form of PS, at rates higher than the legally permitted maximum of 170 kg N ha⁻¹ year⁻¹, may result in better yields. However, high rates of PS fertilization may originate in significantly lower N use efficiency and a higher potential environmental impact in double-cropping systems, practiced in rainfed sub-humid Mediterranean conditions.     

Maize yield and water balance is affected by nitrogen application in a film-mulching ridge–furrow system in a semiarid region of China

Poor soil and drought stress are common in semiarid areas of China, but maize has a high demand for nitrogen (N) and water. Maize production using the technique of double ridges and furrows mulched with plastic film are being rapidly adopted due to significant increases in yield and water use efficiency (WUE) in these areas. This paper studied N use and water balance of maize crops under double ridges and furrows mulched with plastic-film systems in a semiarid environment over four growing seasons from 2007 to 2010. To improve precipitation storage in the non-growing season, the whole-year plastic-film mulching technique was used. There were six treatments which had 0, 70, 140, 280, 420 or 560 kg N ha⁻¹ applied in every year for maize. In April 2011, spring wheat was planted in flat plots without fertilizer or mulch following four years of maize cultivation. After four years, all treatments not only maintained soil water balance in the 0–200 cm soil layer but soil water content also increased in the 0–160 cm soil layer compared to values before maize sowing in April 2007. However, under similar precipitation and only one season of spring wheat, soil water content in the 0–160 cm soil layer sharply decreased in all treatments compared to values before sowing in April 2011. Over the four years of maize cultivation, average yield in all treatments ranged from 4071 to 6676 kg ha⁻¹ and WUE ranged from 18.2 to 28.2 kg ha⁻¹ mm⁻¹. In 2011, the yield of spring wheat in all treatments ranged from 763 to 1260 kg ha⁻¹ and WUE from 3.5 to 6.5 kg ha⁻¹ mm⁻¹. The potential maximum grain yield for maize was 6784 kg ha⁻¹ with 360 kg N ha⁻¹ applied for four years, but considerable NO₃N accumulated in the soil profile. A lesser application (110 kg N ha⁻¹) to this tillage system yielded in 82% of the maximum, increased nitrogen use efficiency and mitigated the risk of nitrogen loss from the system. This study suggests that double ridge–furrow and whole-year plastic-film mulching could sustain high grain yields in maize with approximately 110 kg N ha⁻¹ and maintain soil water balance when annual precipitation is >273 mm in this semiarid environment.     

Modelling photosynthetic efficiency (α) for the light-response curve of cocksfoot leaves grown under temperate field conditions

Net photosynthetic rate was measured from the youngest fully expanded leaves of field grown cocksfoot (Dactylis glomerata L.) in open pastures and under trees in the Lincoln University silvopastoral experiment (New Zealand). The photosynthetic efficiency (α) and convexity (θ) of the light-response curve were derived from 209 fitted non-rectangular hyperbola functions. There was no relationship between θ and any of the environmental or management variables with a stable mean value of 0.96. For α, individual functions were required for temperature (10–31 °C), nitrogen (N) concentration (1.5–5.9% N), water status (expressed as pre-dawn leaf water potential, ψ_lp) (−0.01 to −1.6 MPa), regrowth duration (20–60 days), and different times (up to 180 min) under moderate (850–950 μmol m⁻² s⁻¹ photon irradiance) and severe (85–95 μmol m⁻² s⁻¹ photon irradiance) shade. The highest α of 0.036 μmol CO₂/μmol photon irradiance was found in non-limiting conditions and defined as the standardised maximum (α_s=1). Values of α_s=1 were measured in optimum ranges of 10–24 °C, 4.0–5.9% N, −0.01 to −1.0 MPa and 20 days regrowth. In addition, values of α_s reached a steady-state asymptote of 0.74 after 60 min of severe shade and 0.92 after 40 min of moderate shade. Individual functions of α could not be integrated into a simple multiplicative model but a ‘law of the minimum factor’ model was appropriate. Predicted results from this model were then validated with 46 independent data points collected when at least two factors were outside their optimum range. The model accounted for 88% of the variation in observed α values. This research has derived functional relationships for α that can be used to assist predictions of leaf photosynthesis and ultimately pasture growth by their inclusion in canopy photosynthesis models.     

Influence of harvest time and frequency on light interception and biomass yield of festulolium and tall fescue cultivated on a peatland

In this study, we report efficiencies of light capture and biomass yield of festulolium and tall fescue cultivated on a riparian fen in Denmark under different harvesting managements. Green biomass targeted for biogas production was harvested either as two cuts (2C) or three cuts (3C) in a year. Three different timings of the first cut in the 2C systems were included as early (2C-early), middle (2C-mid) and late (2C-late) cuts corresponding to pre-heading, inflorescence emergence and flowering stages, respectively. The fraction of intercepted photosynthetically active radiation (f_PAR) was derived from the canopy reflectance measured on 61 dates throughout a year, and cumulative interception of PAR (IPAR) and radiation use efficiency (RUE) was calculated. The dynamics of f_PAR and biomass accumulations was similar for both crops before the first cuts in all managements. Festulolium f_PAR in 2C-early and 2C-mid managements declined faster than in 2C-late and 3C managements in the second growth period and thus growing period IPAR of 2C-early and 2C-mid declined by 8% as compared to 3C management where IPAR was 925 MJ m⁻². Annual festulolium dry matter (DM) yield in 2C-early and 2C-mid managements (average 14.1 Mg DM ha⁻¹) decreased by 22% compared to 3C management (18.2 Mg DM ha⁻¹). The highest and the lowest RUE of festulolium were observed in 3C and 2C-mid managements as 1.97 and 1.59 g MJ⁻¹, respectively. For tall fescue f_PAR declined rather slowly in the second growing period in all 2C managements, which contributed to similar IPAR (908–919 MJ m⁻²), total biomass yield (16.4–18.8 Mg DM ha⁻¹ yr⁻¹) and RUE (1.80–2.07 g MJ⁻¹) for all managements. Whereas both crops were highly productive under both 3C management and 2C management with first harvest after flowering (i.e., 2C-late), the 2C-late strategy is recommended as the least intensive of the two management systems.     

Forage production,quality and water-use-efficiency of four warm-season annual crops at three sowing times in the Loess Plateau region of China

Increasing demand for livestock products is driving development of livestock systems worldwide. That requires improved and new forage production options. The Loess Plateau region in central-northern China is an important area for livestock production, as it supports11% and 19% of the country’s cattle and sheep, respectively (China statistical yearbook 2014). The rain-fed semi-arid environment of the Loess Plateau means that maximizing the water-use-efficiency (WUE) of forage production is vital to guarantee enough fodder supply the livestock demand. A three-year field experiment in north-west Loess Plateau compared forage production, water use and water-use-efficiency as well as crude protein (CP) content of forage maize, Sudan grass, foxtail millet and Japanese millet sown at three sowing dates according to the opening rain during 2011–2013. On average, forage maize produced the highest biomass (12.1 t ha⁻¹) and had the highest WUE (43.4 kg DM ha⁻¹ mm⁻¹). This was followed by Sudan grass (7.8 t ha⁻¹; 26.5 kg DM ha⁻¹ mm⁻¹), Japanese millet (6.7 t ha⁻¹; 26.2 kg DM ha⁻¹ mm⁻¹) and foxtail millet (6.7 t ha⁻¹; 24.6 kg DM ha⁻¹ mm⁻¹). Optimizing sowing date played an important role in maximizing forage production and WUE of all tested forages. Compared to the earliest sowing date, a delay of two weeks reduced forage production by 17% in maize, 35% in foxtail millet, and 16% in Japanese millet. A delay of four to six weeks reduced biomass yield by 58% in maize, 57% in foxtail millet, and 56% in Japanese millet. Late sowing also greatly reduced WUE of forage maize and foxtail millet by 33% and 42%, respectively, when compared to early sowing. The middle sowing date maximized forage production and WUE of Sudan grass in two of the three growing seasons, which was 20% and 38% higher than the early and late sowing, respectively. Late sowing in all forages reduced crop water use by 42–57 mm compared to the early sowing. Among four test crops, CP of Sudan grass (7.9%) and forage maize (7.7%) was higher than foxtail millet (6.8%) and Japanese millet (6.7%). Compared with early sowing, CP_f in late sowing significantly increased in Sudan grass and decreased in Japanese millet, in contrast, no evident sowing date effect was found in forage maize and foxtail millet. This study showed that all four warm-season annual grasses had high forage production potential, forage maize was the most reliable and efficient option. Forage maize and the millets could easily be integrated into existing cropping systems and provide opportunities as both grain and forage-producing crop to provide added flexibility for farmers.     

Performance of fogged disinfectants to inactivate conidia of Penicillium digitatum within citrus degreening rooms

Fogging with formaldehyde of citrus packinghouses when the fruit are absent is a practice to control conidia of Penicillium digitatum (Pers.) Sacc., the cause of citrus green mold. Replacements for formaldehyde in these facilities are needed because of worker and environmental health issues. To evaluate the effectiveness of candidate sanitizers, craft wood sticks with conidia of P. digitatum were attached throughout commercial citrus ethylene degreening rooms and either water alone or the sanitizers were applied. The rooms were 20 ± 2 °C and humidified to 85–90% relative humidity. Aldehydes, peroxygen compounds, sodium hypochlorite, chlorine dioxide, quaternary ammonium, alcohols, one phenolic compound, and one organic acid were applied with a compressed air assisted atomizer or fan atomizer in a volume of approximately 6 L per 100 m³ of room volume dispensed over a 3 h period. Rates applied were expressed as active ingredient per m³ of room volume. All were compared to formaldehyde applied at 1.98 g m⁻³ of room volume. After 24 h, the craft wood sticks were retrieved, and germination of the conidia assessed. Five sanitizers reduced germination of conidia by more than 95% and equaled formaldehyde in effectiveness. They were (effective rates): (1) glutaraldehyde (0.1 g m⁻³); (2) hydrogen peroxide (4.4 g m⁻³); (3) Citrisol (1.0 g m⁻³), a proprietary mineral oxychloride oxidizer; (4) acetic acid (5.3 g m⁻³); and (5) peracetic acid (2.4 g m⁻³). The toxicity of effective sanitizers was determined by exposure of P. digitatum conidia for 10 min to concentrations of each and the proportion of survivors used to estimate EC₅₀ and EC₉₉ concentrations. The toxicity of the sanitizers in this assay did not predict their effectiveness when applied by fogging, probably because other factors, such as distribution, persistence, droplet size, or vapor pressure also influenced their effectiveness.     

Application of low intensity light pulses to delay postharvest senescence of Ocimum basilicum leaves

Fresh basil (Ocimum basilicum L.) is a highly perishable leafy green vegetable with a storage life of 4–5 d at room temperature. Exposure of basil leaves to temperatures below 12 °C during storage results in chilling injury; therefore, refrigeration cannot be used to extend postharvest life of basil. Typically, leafy vegetables are stored in darkness or extremely low irradiance. Darkness is known to induce senescence, and the initial phase of senescence is reversible by exposure to light. In this work, we studied the effects of low-intensity white light pulses at room temperature on postharvest senescence of basil leaves. Daily exposure for 2 h to 30–37 μmol m⁻² s⁻¹ of light was effective to delay postharvest senescence of basil leaves. Chlorophyll and protein levels decreased, ammonium accumulated and leaves developed visual symptoms of deterioration (darkening) during storage in darkness. Light pulses reduced the intensity of these senescence symptoms. The photosynthesis light compensation point of basil leaves was 50 μmol m⁻² s⁻¹ i.e., higher than the intensity used in this study, and the effect of treatment with red light was the same as with white light, while far red light was ineffective. Light pulses exerted a local effect on chlorophyll loss, but the effect on protein degradation was systemic (i.e., spreading beyond the illuminated parts of the leaf blade). The results of this study indicate that daily treatment for 2 h with low intensity light (30–37 μmol m⁻² s⁻¹ every day) during storage at 20 °C is an effective treatment to delay postharvest senescence of basil leaves. The delay of postharvest senescence by low intensity light pulses seems to be mediated by phytochromes, and it is systemic for protein, and partially systemic for chlorophyll degradation.     

Evaluation of nitrogen management in maize cultivation grows on soil amended with sewage sludge and urea

This paper analyses the data of a 3 years’ research on the agronomical use of sewage sludge, from a urban waste water plan, to grow maize (Zea mays L.). The experiment was conducted in order to test possible combinations of sewage sludge and urea as source of nitrogen for maize. The experiment comprised a randomized block design composed of a control and 8 treatments with four replicates. Three urea rates (0; 100 kg N ha⁻¹ and 200 kg N ha⁻¹) were assigned combined with three sewage sludge rates (0; 5 t ha⁻¹ and 10 t ha⁻¹), exceeding the limits permitted by the law, and the unfertilized control. Maize was sown and harvested for 2 years (April–September 2006 and 2007) and wheat (Triticum vulgare L.) was sown in October 2007 and harvested in May 2008 without adding any fertilizer or sewage sludge, in order to evaluate the residual effects of the organic fertilizer.The batch that gave the highest grain production was the one that received 10 t ha⁻¹ DM of sewage sludge and 100 kg N ha⁻¹ from urea, reaching values of 16.17 ± 0.97 t ha⁻¹ DM in the first year and 17.52 ± 0.68 t ha⁻¹ DM in the second one, while the effect of the organic fertilization was still available where wheat was grown. ANR values showed a significant increase between the first and the second year: the average value for the treatment 3 (exclusive use of sludge in maximum dose) has shown an increase from 24.3% in 2006 to 63.4% in 2007, highlighting the effect of the sewage sludge. Yields and nitrogen uptake during and after the experiment and the nitrate losses by leachates have been evaluated: linear correlations were statistically significant, with an improvement in the second year of the trial, between yields and the nitrogen applied (R² = 0.757) and yields and the nitrogen removal rate (R² = 0.843).     

Energy of biomass sorghum irrigated with reclaimed wastewaters

The sustainability of biomass sorghum (Sorghum bicolor L. Moench) in the Mediterranean environments is linked to the potential to increasing the crop productivity using irrigation water of different qualities: fresh and wastewater. An experiment was conducted in Southern Italy during 2012 and 2013 growing seasons to determine the biomass production and to estimate the yielded energy from sorghum irrigated with fresh water and municipal wastewaters. Two stages of wastewater reclamation process were compared: tertiary and secondary treatments.During the growing seasons, the crop growth (biomass and LAI) was surveyed on sorghum crops irrigated with three water qualities. In order to determine the effects of the irrigation water qualities on the final energy yielded, on the harvested biomass, structural components (cellulose, hemicellulose and lignin contents for deriving the ethanol production) and high heating value were analyzed. The data obtained during two crop seasons showed that, sorghum irrigated with municipal wastewater plant produced more dry biomass (23.3 vs 20.3 t ha⁻¹), energy yield (383 vs 335 GJ ha⁻¹), and ethanol (6824 vs 6092 L ha⁻¹) than sorghum biomass with fresh water. As a consequence, the water efficiency for producing bioenergy increased when the waste waters were supplied in substitution of fresh waters. Different indices were calculated for comparing the effect of the water quality on the water use efficiency (WUE) of biomass sorghum crops.     

Effects of low temperature storage and sucrose pulsing on the vase life of Lilium cv. Brindisi inflorescences

Lilium cv. Brindisi inflorescences were stored at 2.5 °C for 5, 10, 15 or 20 d, comparing dry storage with storage of the stem ends in water. Prior to storage, inflorescences were treated with 20 or 100 g L⁻¹ sucrose in water, for 20 h at 20 °C. After storage the inflorescences were individually placed in water at 20 °C. The floral buds were still closed at the end of cold storage. In experiments carried out in summer, the time to bud opening was hastened by storage at 2.5 °C in water, more so after a longer period of cold storage. The time to tepal senescence after cold storage in water decreased with the time of storage. The time to tepal abscission was about 1 day longer than the time to tepal senescence. Repeat experiments in late fall and winter additionally showed early leaf yellowing after cold storage. Compared to the experiments in summer, more desiccated floral buds were found in the fall. Pulse treatment with 100 g L⁻¹ sucrose prior to cold storage reduced the number of desiccated buds. However, leaf yellowing was aggravated by the 100 g L⁻¹ sucrose pulse treatment. Compared to cold storage in water, dry storage at 2.5 °C further hastened the time to bud opening and also further hastened tepal senescence and abscission. Dry storage also produced more buds that desiccated or opened poorly. Sucrose treatment (100 g L⁻¹) alleviated the effects of dry storage on tepal senescence and bud desiccation. The data showed that lily cv. Brindisi inflorescences are prone to chilling injury, but can be stored, depending on the treatment, for 5–10 d, during most of the year.     

Improved persistence of red clover (Trifolium pratense L.) increases the protein supplied by red clover/grass swards grown over four harvest years

UK livestock agriculture can significantly reduce its protein imports by increasing the amount of forage based protein grown on-farm. Forage legumes such as red clover (Trifolium pratense L.) produce high dry matter yields of quality forage but currently available varieties lack persistence, particularly under grazing. To assess the impact of red clover persistence on protein yield, diploid red clover populations selected for improved persistence were compared with a range of commercially available varieties. All populations were grown over four harvest years in mixed swards with either perennial ryegrass (Lolium perenne L.) or perennial plus hybrid ryegrass (L. boucheanum Kunth). Red clover and total sward dry matter (DM) herbage yields were measured in Years 1–4, red clover plant survival in Years 3 and 4 and herbage protein (CP) yield and concentration in Years 2 and 4. In general, red clover DM yield in year 4 (3.4 t ha⁻¹) was lower than in year 1 (13.9 t ha⁻¹) but the red clover populations differed in the extent of this decline. Differences in the persistence of the red clover populations in terms of plant survival and yield were reflected in the contribution of red clover to the total sward yield in Year 4, which ranged from 61% for the highest yielding population, AberClaret, to 11% in the lowest yielding, Vivi. Increased red clover DM yield was reflected in a greater CP yield (protein weight per unit area), which ranged from 1.6 t ha⁻¹ year⁻¹ to 2.9 t ha⁻¹ year⁻¹ in Year 2 and from 1.1 t ha⁻¹ year⁻¹ to 1.9 t ha⁻¹ year⁻¹ in Year 4. CP concentration (protein weight per unit herbage weight) of all of the red clover populations was within a range considered suitable for ruminant production. The implication of these results for the future use of red clover in sustainable grassland systems is discussed.     

Use of soil and vegetation spectroradiometry to investigate crop water use efficiency of a drip irrigated tomato

An agronomic research was conducted in Tuscany (Central Italy) to evaluate the effects of an advanced irrigation system on the water use efficiency (WUE) of a tomato crop and to investigate the ability of soil and vegetation spectroradiometry to detect and map WUE. Irrigation was applied following an innovative approach based on CropSense system. Soil water content was monitored at four soil depths (10, 20, 30 and 50 cm) by a probe. Rainfall during the crop cycle reached 162 mm and irrigation water applied with a drip system amounted to 207 mm, distributed with 16 irrigation events. Tomato yield varied from 7.10 to 14.4 kg m⁻², with a WUE ranging from 19.1 to 38.9 kg m⁻³. The irrigation system allowed a high yield levels and a low depth of water applied, as compared to seasonal ET crop estimated with Hargraves’ formula and with the literature data on irrigated tomato. Measurements were carried out on geo-referenced points to gather information on crop (crop yield, eighteen Vegetation indices, leaf area index) and on soil (spectroradiometric and traditional analysis). Eight VIs, out of nineteen ones analyzed, showed a significant relationship with georeferenced yield data; PVI maps seemed able to return the best response, before harvesting, to improve the knowledge of the area of cultivation and irrigation system. CropSense irrigation system reduced seasonal irrigation volumes. Some vegetation indexes were significantly correlated to tomato yield and well identify, a posteriori, crop area with low WUE; spectroradiometry can be a valuable tool to improve irrigated tomato field management.     

Residual effects of pig slurry and mineral nitrogen fertilizer on irrigated wheat

Field experiments conducted in 2007/08 and 2008/09 at the Changwu Agricultural Research Station on the Loess Plateau of China comprised three seeding rates (SR1: 225 seeds m⁻², SR2: 280 seeds m⁻², and SR3: 340 seeds m⁻²) and two root pruning treatments (W: root pruning in the over-wintering period and S: root pruning at the spring-growth stage), with the un-pruned wheat plants as controls. In the severe drought toward the end of the growing season of 2008, grain yield decreased as the seeding rate increased, but under the more favorable conditions in 2009 the reverse was true. Averaged over the seeding rates, grain yield was significantly increased in both W and S in both years; grain yield and yield components were higher in W; and S recorded the highest water use efficiency. The interaction between seeding rate and root pruning was not statistically significant. Leaf area index (LAI) and tiller density were higher as seeding rates increased whereas in W and S, increased LAI and decreased tillers significantly, but had no effect on fertile tillers. The rate of leaf photosynthesis was lower and root respiration was significantly higher at higher seeding rates, whereas in root pruning treatments, significantly higher leaf photosynthetic rate and lower root respiration were observed. Soil water contents were lower as seeding rate increased. A significant decrease in water use before stem elongation was observed in W, while S consumed less soil water than W and the control over the whole growing season. Post-heading accumulation of dry matter and its remobilization from vegetative parts to the grain was significantly greater at higher seeding rates. Post-heading accumulations of dry matter and grain yield were also significantly greater in W and S than the un-pruned plants, although pruning reduced both dry matter remobilization and its contribution to grain yield. The possibility of reducing the proliferation of roots to increase yields at higher seeding rates and conserving the soil water at different growing stages in water-limited environments is discussed.