Water,land, and energy use efficiencies and financial evaluation of air conditioner cooled greenhouses based on field experiments

High temperature and humidity can be controlled in greenhouses by using mechanical refrigeration cooling system such as air conditioner(AC)in warm and humid regions.This study aims to evaluate the techno-financial aspects of the AC-cooled greenhouse as compared to the evaporative cooled(EV-cooled)greenhouse in winter and summer seasons.Two quonset single-span prototype greenhouses were built in the Agriculture Experiment Station of Sultan Qaboos University,Oman,with dimensions of 6.0 m long and 3.0 m wide.The AC-cooled greenhouse was covered by a rockwool insulated polyethylene plastic sheet and light emitting diodes(LED)lights were used as a source of light,while the EV-cooled greenhouse was covered by a transparent polyethylene sheet and sunlight was used as light source.Three cultivars of high-value lettuce were grown for experimentation.To evaluate the technical efficiency of greenhouse performance,we conducted measures on land use efficiency(LUE),water use efficiency(WUE),gross water use efficiency(GWUE)and energy use efficiency(EUE).Financial analysis was conducted to compare the profitability of both greenhouses.The results showed that the LUE in winter were 10.10 and 14.50 kg/m² for the AC-and EV-cooled greenhouses,respectively.However,the values reduced near to 6.80 kg/m² in both greenhouses in summer.The WUE of the AC-cooled greenhouse was higher than that of the EV-cooled greenhouse by 3.8%in winter and 26.8%in summer.The GWUE was used to measure the total yield to the total greenhouse water consumption including irrigation and cooling water;it was higher in the AC-cooled greenhouse than in the EV-cooled greenhouse in both summer and winter seasons by almost 98.0%–99.4%.The EUE in the EV-cooled greenhouse was higher in both seasons.Financial analysis showed that in winter,gross return,net return and benefit-to-cost ratio were better in the EVcooled greenhouse,while in summer,those were higher in the AC-cooled greenhouse.The values of internal rate of return in the AC-and EV-cooled greenhouses were 63.4%and 129.3%,respectively.In both greenhouses,lettuce investment was highly sensitive to changes in price,yield and energy cost.The financial performance of the AC-cooled greenhouse in summer was better than that of the EV-cooled greenhouse and the pattern was opposite in winter.Finally,more studies on the optimum LED light intensity for any particular crop have to be conducted over different growing seasons in order to enhance the yield quantity and quality of crop.     

Identifying water vapor sources of precipitation in forest and grassland in the north slope of the Tianshan Mountains,Central Asia

Identifying water vapor sources in the natural vegetation of the Tianshan Mountains is of significant importance for obtaining greater knowledge about the water cycle, forecasting water resource changes, and dealing with the adverse effects of climate change. In this study, we identified water vapor sources of precipitation and evaluated their effects on precipitation stable isotopes in the north slope of the Tianshan Mountains, China. By utilizing the temporal and spatial distributions of preci...     

Effect of the W-beam central guardrails on wind-blown sand deposition on desert expressways in sandy regions

Many desert expressways are affected by the deposition of the wind-blown sand,which might block the movement of vehicles or cause accidents.W-beam central guardrails,which are used to improve the safety of desert expressways,are thought to influence the deposition of the wind-blown sand,but this has yet not to be studied adequately.To address this issue,we conducted a wind tunnel test to simulate and explore how the W-beam central guardrails affect the airflow,the wind-blown sand flux and the deposition of the wind-blown sand on desert expressways in sandy regions.The subgrade model is 3.5 cm high and 80.0 cm wide,with a bank slope ratio of 1:3.The W-beam central guardrails model is 3.7 cm high,which included a 1.4-cm-high W-beam and a 2.3-cm-high stand column.The wind velocity was measured by using pitot-static tubes placed at nine different heights(1,2,3,5,7,10,15,30 and 50 cm)above the floor of the chamber.The vertical distribution of the wind-blown sand flux in the wind tunnel was measured by using the sand sampler,which was sectioned into 20 intervals.In addition,we measured the wind-blown sand flux in the field at K50 of the Bachu-Shache desert expressway in the Taklimakan Desert on 11 May 2016,by using a customized 78-cm-high gradient sand sampler for the sand flux structure test.Obstruction by the subgrade leads to the formation of two weak wind zones located at the foot of the windward slope and at the leeward slope of the subgrade,and the wind velocity on the leeward side weakens significantly.The W-beam central guardrails decrease the leeward wind velocity,whereas the velocity increases through the bottom gaps and over the top of the W-beam central guardrails.The vertical distribution of the wind-blown sand flux measured by wind tunnel follows neither a power-law nor an exponential function when affected by either the subgrade or the W-beam central guardrails.At 0.0H and 0.5H(where H=3.5 cm,which is the height of the subgrade),the sand transport is less at the 3 cm height from the subgrade surface than at the 1 and 5 cm heights as a result of obstruction by the W-beam central guardrails,and the maximum sand transportation occurs at the 5 cm height affected by the subgrade surface.The average saltation height in the presence of the W-beam central guardrails is greater than the subgrade height.The field test shows that the sand deposits on the overtaking lane leeward of the W-beam central guardrails and that the thickness of the deposited sand is determined by the difference in the sand mass transported between the inlet and outlet points,which is consistent with the position of the minimum wind velocity in the wind tunnel test.The results of this study could help us to understand the hazards of the wind-blown sand onto subgrade with the W-beam central guardrails.     

Optimal bandwidth selection for retrieving Cu content in rock based on hyperspectral remote sensing

Hyperspectral remote sensing technology is widely used to detect element contents because of its multiple bands,high resolution,and abundant information.Although researchers have paid considerable attention to selecting the optimal bandwidth for the hyperspectral inversion of metal element contents in rocks,the influence of bandwidth on the inversion accuracy are ignored.In this study,we collected 258 rock samples in and near the Kalatage polymetallic ore concentration area in the southwestern part of Hami City,Xinjiang Uygur Autonomous Region,China and measured the ground spectra of these samples.The original spectra were resampled with different bandwidths.A Partial Least Squares Regression(PLSR)model was used to invert Cu contents of rock samples and then the influence of different bandwidths on Cu content inversion accuracy was explored.According to the results,the PLSR model obtains the highest Cu content inversion accuracy at a bandwidth of 35 nm,with the model determination coefficient(R²)of 0.5907.The PLSR inversion accuracy is relatively unaffected by the bandwidth within 5-80 nm,but the accuracy decreases significantly at 85 nm bandwidth(R²=0.5473),and the accuracy gradually decreased at bandwidths beyond 85 nm.Hence,bandwidth has a certain impact on the inversion accuracy of Cu content in rocks using the PLSR model.This study provides an indicator argument and theoretical basis for the future design of hyperspectral sensors for rock geochemistry.     

Assessing the collapse risk of Stipa bungeana grassland in China based on its distribution changes

The criteria used by International Union for Conservation of Nature(IUCN) for its Red List of Ecosystems(RLE) are the global standards for ecosystem-level risk assessment, and they have been increasingly used for biodiversity conservation. The changed distribution area of an ecosystem is one of the key criteria in such assessments. The Stipa bungeana grassland is one of the most widely distributed grasslands in the warm-temperate semi-arid regions of China. However, the total distribution area of this grassland was noted to have shrunk and become fragmented because of its conversion to cropland and grazing-induced degradation. Following the IUCN-RLE standards, here we analyzed changes in the geographical distribution of this degraded grassland, to evaluate its degradation and risk of collapse. Past(1950-1980) distribution areas were extracted from the Vegetation Map of China(1:1,000,000). Present realizable distribution areas were equated to these past areas minus any habitat area losses. We then predicted the grassland’s present and future(under the Representative Concentration Pathway 8.5 scenario) potential distribution areas using maximum entropy algorithm(MaxEnt), based on field survey data and nine environmental layers. Our results showed that the S. bungeana grassland was mainly distributed in the Loess Plateau, Hexi Corridor, and low altitudes of the Qilian Mountains and Longshou Mountain. This ecosystem occurred mainly on loess soils, kastanozems, steppe aeolian soils and sierozems. Thermal and edaphic factors were the most important factors limiting the distribution of S. bungeana grassland across China. Since 56.1% of its past distribution area(4.9×10~4 km^2) disappeared in the last 50 a, the present realizable distribution area only amounts to 2.2×10~4 km^2. But only 15.7% of its present potential distribution area(14.0×10~4 km^2) is actually occupied by the S. bungeana grassland. The future potential distribution of S. bungeana grassland was predicted to shift towards northwest, and the total area of this ecosystem will shrink by 12.4% over the next 50 a under the most pessimistic climate change scenario. Accordingly, following the IUCN-RLE criteria, we deemed the S. bungeana grassland ecosystem in China to be endangered(EN). Revegetation projects and the establishment of protected areas are recommended as effective ways to avert this looming crisis. This empirical modeling study provides an example of how IUCN-RLE categories and criteria may be valuably used for ecosystem assessments in China and abroad.     

conomic losses from reduced freshwater under future climate scenarios: An example from the Urumqi River,Tianshan Mountains

As important freshwater resources in alpine basins,glaciers and snow cover tend to decline due to climate warming,thus affecting the amount of water available downstream and even regional economic development.However,impact assessments of the economic losses caused by reductions in freshwater supply are quite limited.This study aims to project changes in glacier meltwater and snowmelt of the Urumqi River in the Tianshan Mountains under future climate change scenarios(RCP2.6(RCP,Representative Concentration Pathway),RCP4.5,and RCP8.5)by applying a hydrological model and estimate the economic losses from future meltwater reduction for industrial,agricultural,service,and domestic water uses combined with the present value method for the 2030 s,2050 s,2070 s,and 2090 s.The results indicate that total annual glacier meltwater and snowmelt will decrease by 65.6%and 74.5%under the RCP4.5 and RCP8.5 scenarios by the 2090 s relative to the baseline period(1980-2010),respectively.Compared to the RCP2.6 scenario,the projected economic loss values of total water use from reduced glacier meltwater and snowmelt under the RCP8.5 scenario will increase by 435.10×10⁶ and 537.20×10⁶ CNY in the 2050 s and 2090 s,respectively,and the cumulative economic loss value for 2099 is approximately 2124.00×10⁶ CNY.We also find that the industrial and agricultural sectors would likely face the largest and smallest economic losses,respectively.The economic loss value of snowmelt in different sectorial sectors is greater than that of glacier meltwater.These findings highlight the need for climate mitigation actions,industrial transformation,and rational water allocation to be considered in decision-making in the Tianshan Mountains in the future.     

Coupling between the Grain for Green Program and a household level-based agricultural eco-economic system in Ansai,Shaanxi Province of China

The implementation of the Grain for Green Program(GGP)has changed the development track of the agricultural eco-economic system in China.In response to the results of a lag study that investigated the coupling between the GGP and the agricultural eco-economic system in a loess hilly region,we used a structural equation model to analyze the survey data from 494 households in Ansai,a district of Yan’an City in Shaanxi Province of China in 2015.The model clarified the direction and intensity of the coupling between the GGP and the agricultural eco-economic system.The coupling benefits were derived through linkages between the program and various chains in the agricultural eco-economic system.The GGP,the agroecosystem of Ansai and their potential coupling effects were in a state of general coordination.The agroecosystem directly affected the coupling effect,with the standardized path coefficient of 0.87,indicating that the agroecosystem in Ansai at this stage provided basic material support for the coupling between the GGP and the agricultural eco-economic system.The direct path coefficient of agroeconomic system impacted on the coupling effect was-0.76,indicating that partial contradictions occurred between the agroeconomic system and the coupling effect.Therefore,although the current agroecosystem in Ansai should be provided sufficient agroecological resources for the benign coupling between the program and the agricultural eco-economic system,agricultural development failed to effectively transform agroecological resources into agricultural economic advantages in this region,which resulted in a relative lag in the development of the agricultural economic system.Thus,the coupling between the GGP and the agricultural eco-economic system was poor.To improve the coupling and the sustainable development of the agricultural eco-economic system in cropland retirement areas,the industrial structure needs to be diversified,the agricultural resources(including agroecological resources,agricultural economic resources and agricultural social resources)need to be rationally allocated,and the chain structure of the agricultural eco-economic system needs to be continuously improved.     

Effects of long-term warming on the aboveground biomass and species diversity in an alpine meadow on the Qinghai-Tibetan Plateau of China

Ecosystems in high-altitude regions are more sensitive and respond more rapidly than other ecosystems to global climate warming.The Qinghai-Tibet Plateau(QTP)of China is an ecologically fragile zone that is sensitive to global climate warming.It is of great importance to study the changes in aboveground biomass and species diversity of alpine meadows on the QTP under predicted future climate warming.In this study,we selected an alpine meadow on the QTP as the study object and used infrared radiators as the warming device for a simulation experiment over eight years(2011-2018).We then analyzed the dynamic changes in aboveground biomass and species diversity of the alpine meadow at different time scales,including an early stage of warming(2011-2013)and a late stage of warming(2016-2018),in order to explore the response of alpine meadows to short-term(three years)and long-term warming(eight years).The results showed that the short-term warming increased air temperature by 0.31℃and decreased relative humidity by 2.54%,resulting in the air being warmer and drier.The long-term warming increased air temperature and relative humidity by 0.19℃and 1.47%,respectively,and the air tended to be warmer and wetter.The short-term warming increased soil temperature by 2.44℃and decreased soil moisture by 12.47%,whereas the long-term warming increased soil temperature by 1.76℃and decreased soil moisture by 9.90%.This caused the shallow soil layer to become warmer and drier under both short-term and long-term warming.Furthermore,the degree of soil drought was alleviated with increased warming duration.Under the long-term warming,the importance value and aboveground biomass of plants in different families changed.The importance values of grasses and sedges decreased by 47.56%and 3.67%,respectively,while the importance value of weeds increased by 1.37%.Aboveground biomass of grasses decreased by 36.55%,while those of sedges and weeds increased by 8.09%and 15.24%,respectively.The increase in temperature had a non-significant effect on species diversity.The species diversity indices increased at the early stage of warming and decreased at the late stage of warming,but none of them reached significant levels(P>0.05).Species diversity had no significant correlation with soil temperature and soil moisture under both short-term and long-term warming.Soil temperature and aboveground biomass were positively correlated in the control plots(P=0.014),but negatively correlated under the long-term warming(P=0.013).Therefore,eight years of warming aggravated drought in the shallow soil layer,which is beneficial for the growth of weeds but not for the growth of grasses.Warming changed the structure of alpine meadow communities and had a certain impact on the community species diversity.Our studies have great significance for the protection and effective utilization of alpine vegetation,as well as for the prevention of grassland degradation or desertification in high-altitude regions.     

Characteristics and hazards of different snow avalanche types in a continental snow climate region in the Central Tianshan Mountains

Snow avalanches are a common natural hazard in many countries with seasonally snow-covered mountains.The avalanche hazard varies with snow avalanche type in different snow climate regions and at different times.The ability to understand the characteristics of avalanche activity and hazards of different snow avalanche types is a prerequisite for improving avalanche disaster management in the mid-altitude region of the Central Tianshan Mountains.In this study,we collected data related to avalanche,snowpack,and meteorology during four snow seasons(from 2015 to 2019),and analysed the characteristics and hazards of different types of avalanches.The snow climate of the mid-altitude region of the Central Tianshan Mountains was examined using a snow climate classification scheme,and the results showed that the mountain range has a continental snow climate.To quantify the hazards of different types of avalanches and describe their situation over time in the continental snow climate region,this study used the avalanche hazard degree to assess the hazards of four types of avalanches,i.e.,full-depth dry snow avalanches,full-depth wet snow avalanches,surface-layer dry snow avalanches,and surface-layer wet snow avalanches.The results indicated that surface-layer dry snow avalanches were characterized by large sizes and high release frequencies,which made them having the highest avalanche hazard degree in the Central Tianshan Mountains with a continental snow climate.The overall avalanche hazard showed a single peak pattern over time during the snow season,and the greatest hazard occurred in the second half of February when the snowpack was deep and the temperature increased.This study can help the disaster and emergency management departments rationally arrange avalanche relief resources and develop avalanche prevention strategies.     

Transformation of vegetative cover on the Ustyurt Plateau of Central Asia as a consequence of the Aral Sea shrinkage

The gradual shrinkage of the Aral Sea has led to not only the degradation of the unique environments of the Aral Sea,but also numerous and fast developing succession processes in the neighborhood habitats surrounding the sea.In this study,we investigated the vegetative succession processes related to the Aral Sea shrinkage in the Eastern Cliff of the Ustyurt Plateau in Republic of Uzbekistan,Central Asia.We compared the results of our current investigation(2010–2017)on vegetative communities with the geobotany data collected during the 1970s(1970–1980).The results showed great changes in the mesophytic plant communities and habitat aridization as a result of the drop in the underground water level,which decreased atmospheric humidity and increased the salt content of the soil caused by the shrinkage of the Aral Sea.In the vegetative communities,we observed a decrease in the Margalef index(DMg),which had a positive correlation with the poly-dominance index(I-D).The main indications of the plant communities'transformation were the loss of the weak species,the appearance of new communities with low species diversity,the stabilization of the projective cover of former resistant communities,as well as the appearance of a new competitive species,which occupy new habitats.     

Systems of designation of pathotypes of plant pathogens12

A variety of systems of designation has evolved to name pathotypes of plant pathogens. The systems were evaluated to determine those best suited for particular purposes. Virulence and avirulence/virulence formulae of pathotypes have advantage over the use of consecutive numbers or letters given in chronological order of pathotype discovery. As soon as pathotype information exceeds a certain level of complexity, mathematical codes are most advantageous, in particular two codes, octal notation and coded triplets. A more universal adoption of the most appropriate codes is recommended to ease communication and comparisons of results.     

Investigation of the Mode of Action of Nematode Neuropeptides

The neuropeptide AF2 has a complex set of actions on the dorsal muscle strip of Ascaris suum, including a potentiation of the acetylcholine-stimulated muscle contraction. Caffeine at 100 μm and 5 mm inhibited this potentiation, as did 100 μm theophylline in two out of six studies. The cyclic-AMP-potentiating compounds IBMX, dibutyryl cAMP and forskolin had no effect on the AF2-induced potentiation of the acetylcholine-stimulated muscle contraction. These preliminary data suggest that the potentiating action of AF2 is not mediated by a cAMP pathway.     

Localization of metabolic sites of action of herbicides

Time- and concentration-course studies were conducted to determine the effect of thirteen herbicides on photosynthesis, respiration, RNA synthesis, protein synthesis, and lipid synthesis using isolated single leaf cells. Each herbicide was from a different chemical class. Appropriate ¹⁴C-substrates and product purification procedures were used for each process prior to liquid scintillation counting. The most sensitive metabolic site of inhibition was photosynthesis for atrazine, bromacil, dichlobenil, monuron, and paraquat; RNA synthesis for dalapon and dinoseb; protein synthesis for chlorpropham; and lipid synthesis for CDAA, chloramben, 2,4-D, EPTC, and trifluralin. However, with several herbicides, one or more process was almost as sensitive as the one mentioned above. All herbicides inhibited more than one process, and the most sensitive site of inhibition may not be the same process that was inhibited the greatest at the maximum concentration and maximum exposure time used. Therefore, a concept of metabolic sites of action, rather than a primary site of action, appears to be more meaningful for herbicides.     

福寿螺配偶个体大小选择性初步观察

通过野外观察与实验研究,掌握了福寿螺的婚配体制及其配偶选择性规律。福寿螺与多数低等动物一样,其婚配属于乱交制,无固定配偶;雌螺对与其交配的雄螺个体大小没有选择性,而雄螺对雌螺的个体大小有选择性,倾向于与较大个的雌螺交配。     

Effects of timing and method of application of Penicillium oxalicum on efficacy and duration of control of Fusarium wilt of tomato

The effects of timing and method of application of Penicillium oxalicum on the control of fusarium wilt of tomato were investigated. Application of P. oxalicum to tomato seedlings in seedbeds reduced disease caused by Fusarium oxysporum f.sp. lycopersici in a growth chamber by 45–49% and in glasshouse experiments by 22–69%. Disease suppression was maintained for 60–100 days after inoculation with the pathogen in the glasshouse. No disease reduction was observed in tomato plants where P. oxalicum was applied to seeds. Treatment with P. oxalicum did not affect the population of F. oxysporum f.sp. lycopersici in the rhizosphere.     

厚朴病虫害种类的初步调查

采用标准地法和线路调查法,对湖北恩施市新塘乡双河厚朴基地的厚朴病虫害进行了系统调查,记录主要虫害13种,其中叶部害虫9种,枝干害虫2种,根部害虫2种。厚朴主要病害5种。藤壶蚧、厚朴枝角叶蜂和厚朴新丽斑蚜为湖北省首次报道,小绿叶蝉为厚朴新寄主记录种。同时记录了藤壶蚧的天敌6种,其中寄生小蜂2种,瓢虫4种;厚朴新丽斑蚜的天敌昆虫8种。对藤壶蚧、厚朴枝角叶蜂和厚朴苗木根腐病等重要病虫害的发生规律进行了初步调查,同时提出了防治建议。     

百合地下害虫主要种类调查及生物（源）农药筛选

调查了甘肃省临洮县为害百合的地下害虫种类, 并以主要发生为害种类为供试对象, 采用拌土法测定了几种生物（源）农药对主要害虫的室内毒力和致死中时。调查发现小云斑鳃金龟Polyphylla gracilicornis、棕色鳃金龟Holotrichia titanis为主要发生及为害种类, 不同时间发生种类存在差异, 9月份以小云斑鳃金龟幼虫发生为害为主, 发生量占地下害虫总数的66.16%, 平均种群密度为10.48头/m2, 10月份, 则以棕色鳃金龟幼虫发生为主, 发生量占地下害虫总数的96.86%, 平均种群密度为7.79头/m2, 主要取食百合根系和鳞茎, 造成根系数量减少, 鳞茎出现不规则褐色缺口或凹陷斑。药剂筛选结果表明, 1.8%阿维菌素乳油、200亿孢子/g球孢白僵菌粉剂对两种蛴螬均具有较高的杀虫活性和速效性, 药后7 d时, 校正死亡率在82.22%以上, 对小云斑鳃金龟幼虫和棕色鳃金龟幼虫的LC50分别为0.161、0.060 mg/g和5.558×107、0.362×107孢子/g, LT50分别为2.237 d（2.2 mg/g）、1.393 d（2.2 mg/g）和6.645 d（40.0×107孢子/g）、4.940 d（2.0×107孢子/g）, 这两种生物（源）农药对两种蛴螬的LC50和LT50值均略大于两种化学农药25%二嗪磷乳油、40%辛硫磷乳油处理的, 且对小云斑鳃金龟幼虫的杀虫活性均低于对棕色鳃金龟幼虫的杀虫活性, 致死中时则长于对棕色鳃金龟幼虫的致死中时。     

青稞种子带菌检测及杀菌剂消毒处理效果

为了研究青稞种子外部和内部携带真菌情况,比较不同杀菌剂对青稞种子的带菌消毒效果和对幼苗生长的影响,为青稞种子播前包衣处理和种传真菌病害防控提供依据,采用离体平皿法对云南迪庆‘云青1号’、‘云青2号’和‘短白青稞’3个主栽品种进行带菌检测,并对种子进行拌种或浸种处理测定6种杀菌剂对种子消毒效果,分析杀菌剂对种子发芽和幼苗生长的影响。结果表明:供试青稞种子表面携带的优势菌群为青霉(Penicilliumspp.)、镰刀菌(Fusariumspp.);种子内部寄藏的真菌主要为镰刀菌、核腔菌(Pyrenophoraspp.)、附球菌(Epicoccumspp.)、丝核菌(Rhizoctoniaspp.)、链格孢(Alternariaspp.)和木霉(Trichoderma spp.)。青稞不同品种的种子表面及内部携带的真菌种类差异较大。致病性测定表明,镰刀菌对种子萌发和幼苗生长影响最大,后期出现幼苗坏死现象。45%咪鲜胺EW、75%百菌清WP、50%福美双WP对青稞种子携带真菌均有显著抑制作用和消毒效果,50%福美双WP消毒效果最优,达100%;45%咪鲜胺EW、75%百菌清WP、50%福美双WP处理对青稞种子发芽和幼苗生长均无显著影响。     

棉花黄萎病菌致病型的AFLP分析

 选用41个棉花黄萎病菌(Verticillium dahliae)代表菌系,在温室条件下,对4个棉花品种鄂荆1号(感)、中棉所12(耐)、文-5(抗)和唐棉2号(抗)进行致病性测定,结果可将供试菌系分为落叶型与非落叶型2类。选取8对AFLP引物PCR扩增的结果中,统计带型稳定、清晰且有多态性的条带,共169条作系统聚类分析,将上述菌系分为2大类,第一类为非落叶型菌系,包括10个非落叶型菌系和1个过渡菌系;第二类为30个落叶型菌系。根据聚类分析建立树状图,发现菌系与地理来源存在一定的相关性,而依据菌系致病力强弱分类则相关关系不大。选用25对EcoRⅠ和MseⅠ引物组合,对供试的41个V.dahliae进行AFLP扩增,筛选到2对引物E64(GACTGCGTACCAATTCGAC)、M53(GATGAGTCCTGAGTAACCG)和E49(GACTGCGTACCAATTCCAG)、M65(GAT-GAGTCCTGAGTAAGAG),能分别扩增出433bp和110bp2条仅为V.dahliae非落叶型菌系独有的特异片段,可将落叶型与非落叶型菌系分开,这2条特异片段被命名为EM₄₃₃和EM₁₁₀。