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1.
刘念  黄琪琳  李沛  郭江勇  覃先武 《水产学报》2021,45(7):1089-1100
为探究添加不同种类酵母抽提物对生鲜鱼片风味及质构的影响,实验以调理乌鳢鱼片为原料,通过同时蒸馏萃取法(SDE)提取挥发性成分,利用GC-MS联合电子鼻、电子舌对6组调理乌鳢鱼片(空白对照CK组、FA02组、KU012组、FG10组、FA39组和F55组)进行风味成分分析,再结合感官、色度和质构进一步评价了6组调理乌鳢鱼...  相似文献   

2.
Effects of oral administration of l-thyroxine (T4) on growth performance, body composition, and some aspects of nutritional physiology were investigated in two slow-growing air-breathing fish (Channa punctatus and Heteropneustes fossilis) under laboratory conditions (LD 12:12 at 25°C). The results indicate that irrespective of the species, fish fed diets containing lower doses of T4 (up to 50 mg kg−1 of diet in C. punctatus and up to 100 mg kg−1 of diet in H. fossilis) showed significantly (P < 0.05) higher growth (in terms of live weight and length gain, specific growth rate, percentage gain in body weight and condition factor), low feed conversion ratio, high nutrient retention, high apparent protein digestibility, and high digestive enzyme activity. Viscero-somatic (VSI) and hepato-somatic (HSI) values were also high in fish fed on low dietary T4 levels. Liver glycogen levels decreased with the increase in the dietary T4 levels, indicating its utilization during growth. Muscle glycogen levels in H. fossilis coincided with high growth at 100 mg of T4. Observation of the postprandial excretion of metabolites (N–NH4 + and o-PO4 ) indicated significantly (P < 0.05) low levels in aquaria water where the fish were fed diets with low T4 levels. These studies further revealed that feeding fishes on higher T4 levels (above 50 mg in C. punctatus and above 100 mg in H. fossilis) not only repressed growth performance and nutrient retention, but also affected carcass composition by lowering protein accumulation (muscle and carcass protein) and energy assimilation. These studies revealed a biphasic action of thyroxine, i.e., the hormone at lower doses is anabolic, while at higher doses it acts as a catabolic agent, indicating that feeding fishes on higher doses can be detrimental to their growth and metabolism. In summary, the results of the present study show that feeding H. fossilis and C. punctatus on low doses of T4 enhances growth, decreases excretion of metabolites, and increases nitrogen retention. These observations suggest that T4 supplementation of the diet may have practical utility in the culture of slow-growing fish species.  相似文献   

3.
In the Mediterranean area, wild fish have often been suggested as either the reservoirs of the causative agents or at least the carriers of the pathogens responsible for disease outbreaks in cultured fish. However, no epidemiological investigations on actual pathogen/disease interactions between farmed and wild fish have been conducted even for the most important fish pathogens. Only sporadic isolations and identifications of various pathogens in wild fish have been done and real associations with the pathological conditions that exist within the farm environment and vice versa have not been established. Monogenean ectoparasite Sparicotyle chrysophrii and isopod Ceratothoa oestroides are commonly found in the Mediterranean cage-reared sea bream and sea bass and in the surrounding wild fish population. Both species were recognized as pathogens that seasonally inflict serious losses in fingerlings and juveniles of sea bream and sea bass, being potentially propagated and exchanged during wild and caged fish interaction. In order to evaluate the degree of pathogens transfer between wild and farmed fish, we investigated genetic population structures of these two important parasitic pathogens inferred by mtDNA cytochrome oxidase I locus. Parasites isolated from wild and farmed fish on the two most productive Adriatic fish farms showed genetic heterogeneity, contradicting widely accepted hypothesis of cross-contamination, at least in case of S. chrysophrii and C. oestroides. As far as we know, this is the first report that in a study of pathogen transfer molecular evidence was employed to asses the genetic population structure of shared parasites.  相似文献   

4.
Abstract. Morphological, behavioural and haematological changes in Channa punctatus caused by infection with the digenean Isoparorchis hypselobagri are reported. Morphological changes include partial necrosis of fin tissue, scale loss (particularly along the opercula and caudal peduncle) and loss of pigmentation, together with considerable damage to the viscera, especially the gonads, and the abdominal muscles. Behavioural changes include partial or complete loss of equilibrium, irritability and convulsions. Haematological changes observed were a significant decrease in haemoglobin and haematocrit values resulting in hypochromicmacrocytic and normochromicnormocytic anaemia together with an increase in total leucocyte number. Significant decreases in blood glucose level and liver glycogen content were also observed. Some of the changes could be partially compensated by providing a bigger food ration. Compensatory effects were observed for haemoglobin and haematocrit values and leucocyte number. However, an increase in diet could not arrest the significant decrease in blood glucose level and liver glycogen content. The possible effects of parasitism on the utilization and productivity of C. punctatus are discussed.  相似文献   

5.
Dietary arginine requirement of Heteropneustes fossilis fry (3.0 ± 0.5 cm; 5.1 ± 0.3 g) was determined by feeding casein‐gelatin‐based isonitrogenous (400 g kg?1 crude protein) and isocaloric (17.97 kJ g?1) amino acid test diets containing graded levels of l ‐arginine (15, 17, 19, 21, 23 and 25 g kg?1 dry diet) for 12 weeks. Maximum absolute weight gain (AWG) (44.4), best feed conversion ratio (FCR) (1.22), highest protein retention efficiency (PRE%) (41%), energy retention efficiency (ERE%) (75%), best condition factor, hepatosomatic index and viscerosomatic index were noted at 21 g kg?1 arginine of the dry diet. Maximum body protein (189.8 g kg?1) was also obtained in fish fed above diet. Highest haematocrit value (35%), Hb concentration (9.54 g dL?1), RBC count (3.44 × 109 mL?1) and lowest Erythrocyte sedimentation rate (ESR) (1.93 mm h?1) were obtained at the above level of arginine in the diet. AWG, FCR, PRE% and ERE% data were analysed using broken‐line and an exponential fit to obtain more precise dietary arginine requirement. On the basis of broken‐line and exponential analyses of AWG, FCR, PRE and ERE data, inclusion of dietary arginine in the range of 20.4–22.6 g kg?1 dry diet, corresponding to 51–56.5 g kg?1 dietary protein, is recommended for formulating arginine‐balanced feeds for rearing H. fossilis fry.  相似文献   

6.
Age, growth and maturity data of the freshwater feather back Chitala chitala (Hamilton-Buchanan, 1822) were collected from ten different geographical locations. Out of ten populations, maximum 6+ ages were recorded from four river basins namely river Bhagirathi, Koshi, Saryu and Ganga and the back calculated lengths at 6+ ages ranged from 1033.36–1073.63 mm. In rest of the locations, however, only 3+ age classes were recorded. Specific rate of linear growth (Cl) and specific rate of weight increase (Cw) showed decreases except in two locations. The specific rate of linear growth (Cl) and the specific rate of weight increase (Cw) increased at 3+ age class in the river Bhagirathi. Among other growth parameters, three distinct life stages of C. chitala were observed based on analysis of growth constant data. Analysis of variance (ANOVA) of the back-calculated length data of 10 populations indicated a significant difference (p < 0.05). Out of ten populations studied, six showed significant variation of growth in 1+ age class and two showed significant variation in 2+ to 6+ age classes. Length-weight relationship of the species from all the population was calculated and the coefficient of correlation (r) was at the significant level where the value of ‘b’ was almost 3 for all the locations studied. Male C. chitala attained maturity earlier (2+ age) whereas females matured at age 3+. The percentages of mature individuals also varied between river basins. Based on this study strategies can be proposed for sustainable exploitation of the species from wild populations.  相似文献   

7.
The effects of stress due to handling, and repeated sham and human chorionic gonadotropin (HCG) injections on spawning and survival were studied. Results showed that stress significantly enhanced spawning in captive females (P<0.05), but apparently has no significant effect on the survival of larvae. The results indicate that factors other than stress are responsible for the high variability in larval survival in the hatchery. In addition, the results clearly demonstrate the necessity of exogenous gonadotropin to ensure 100% monthly spawning of captive S. guttatus females.  相似文献   

8.
Growth of newly metamorphosed Macrobrachium australiense was monitored in individually vidually held and group held laboratory animals over 28 weeks. A breeding population was also established in a farm dam and the growth of young juvenile F1 progeny was recorded for a similar time. Up to 26 moults were recorded in this period and intermoult periods increased with age. Prawns held in groups grew better than those held individually. Reproductive maturity was attained by some females in group held laboratory and farm dam animals within the observation period. Length—weight regression equations were calculated for laboratory maintained prawns, i.e., log W = 0.44+ 2.44 log L; log W = 0.45 + 2.46 log L for individually held males and females, respectively; log W = 0.27 + 2.70 log L for group held males and females.  相似文献   

9.
This study was aimed to address the promising evaluation of Cissus quadrangularis plant (stem) and lipopolysaccharide (LPS from bacteria) supplemented diets on innate immune response in Lates calcarifer fingerlings against Aeromonas hydrophila infection. Fingerlings were fed supplemented diets containing four different concentrations of C. quadrangularis (0.5, 1.0, 1.5 and 2.0 g kg?1 feed), LPS (25, 50, 75 and 100 mg kg?1 feed) and control (normal formulated diet) for 60 days. The fish fingerlings fed supplemented diet displayed significant differences (P < 0.05) in specific growth rate (SGR) and relative percentage survival compared to the control group fed without C. quadrangularis and LPS‐supplemented diet. Fingerlings were injected intraperitoneally with 100 μL lethal dose of A. hydrophila containing 1 × 106 CFU g?1. Supplementation of C. quadrangularis and LPS diet significantly increased biochemical profile such as protein, lipid and carbohydrate content, haematological parameters of L. calcarifer fingerlings in different experimental periods when compared with the control group. Dietary doses of C. quadrangularis and LPS‐supplemented diet significantly influenced growth performance and increased survival rate in L. calcarifer fingerlings against A. hydrophila infection.  相似文献   

10.
Dicentrarchus labrax and Dicentrarchus punctatus is able to thrive and grow in fresh water ponds. In all experiments there was successful controlling of young Tilapia aurea in the experimental ponds in comparison to the control ones. The number of young T. aurea per female and per pond was significantly lower in the experimental ponds than in control ponds. In 1973 the average number of young T. aurea per female was 80 in the experimental ponds and 287 young in the control ponds. In 1974 the average number of T. aurea young per female was 48 in the experimental ponds and 586 young in the control ponds.The growth rate of D. punctatus and D. labrax was higher in fresh water (S = 0.5‰) than in nature (salt water).  相似文献   

11.
The main biological characteristics for black scabbardfish in the Canary Islands waters were established, focusing on its reproductive behaviour and strategy and its growth pattern. The sex proportion differs significantly from the 1:1 ratio, being clearly unbalanced towards the females. The sex ratio according to depth clearly shows the presence of male and female individuals in all the studied layers, thus sex segregation does not happen according to depth. The Canaries can be considered to be a reproduction site for A. carbo, since samples showing all stages of maturity have been observed. The gonadosomatic index (GSI) showed that the mean values were higher in the third and fourth quarters of the year. The index values clearly allow the differentiation of the individuals in resting or in spent (II and V) stages from individuals in ripe or in ripe and running stages, indicating that index levels higher than 2 are typical of maturation followed by spawning process. Up to twelve marks, assumed to be annuli, were visible in the sampled otoliths. Three to five years old were the dominant age classes and only 7.7% of fish were 7 years old or older. Age estimates ranged between 2 and 8 years for males and between 2 and 12 years for females. Estimated growth parameters were: L = 1477 ± 18.73 mm; k = 0.200 ± 0.016 year−1; and t0 = −4.58 ± 0.413 year. Biological aspects of the black scabbardfish suggest that this species has a generalist life-history strategy.  相似文献   

12.
We investigated the effects of concentration of the microalga Dunaliella tertiolecta on the growth and survival of fairy shrimp, Phallocryptus spinosa. Newly hatched nauplii were stocked into containers, maintained at different concentrations of D. tertiolecta (at 18, 36, 54, 72 and 90 × 106 cells mL?1). All treatments were in quadruplicate and each replicate was stocked with 100 larvae in a 2‐L cylindrical bowl. We studied the survival and growth of the fairy shrimp after 3, 6, 9, 12 and 15 days of culture. The results indicated significant differences, in terms of growth and survival, of fairy shrimps fed at different algal densities. The highest and lowest growth and survival among the treatments were observed on Day 15, the highest in animals fed at a concentration of 90 × 106 cells mL?1 and the lowest in animals fed at a concentration of 18 × 106 cells mL?1. We conclude that the growth and survival of the P. spinosa increased with increasing density of algae, to a threshold level. Within certain concentration limits, the addition of D. tertiolecta substantially improved the performance of larval culture of P. spinosa, suggesting that this fairy shrimp has potential in terms of aquaculture development.  相似文献   

13.
Black Scorpion fish (Scorpaena porcus Linnaeus 1758) caught during monthly sampling carried out in 2003 in the Dardanelles, Turkey, were examined for the parasite. Trachelobdella lubrica. Infested fish were caught only during December 2003, and the clinical conditions of the 12 fish naturally infested with T. lubrica included fin rot, swollen foci of skin and haemorrhages of the gill and abdominal skin. The prevalence, intensity and abundance of infestation in December 2003 were 40%, 1.75 and 0.70, respectively. The average hepatosomatic index of the infested fish was lower than that of noninfested fish, while the means of the kidney index, splenosomatic index, condition factor, body weight and length were not significantly different between the infested and noninfested fish groups. Blood analysis was conducted on 12 naturally infested and 12 healthy (noninfested) Black Scorpion fish to determine the possible characteristic changes in blood parameters of the fish in response to natural infestation with T. lubrica. Blood leucocyte counts, haematocrit levels, serum total protein, albumin phosphorus, urea, glucose, globulin, cholesterol and triglyceride concentrations in naturally infested Black Scorpion fish were significantly less than those of the noninfested fish (p < 0.05). No significant decreases were observed in the serum sodium, iron, calcium, potassium, chlorine, blood urea nitrogen and creatinine values of the naturally infested fish. Blood haemoglobin values, erythrocyte counts and serum glutamate aspartate aminotransferase (glutamate oxalacetate transaminase), alanine aminotransferase (glutamate pyruvate transaminase), uric acid and very low-density lipids levels of infested fish were insignificantly greater than those in healthy fish.  相似文献   

14.
Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1–5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L?1 as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2 % DMSO solution without azadirachtin and considered as negative control T0?. Along with six treatment groups, a positive control T0+ of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L?1 and that also shifted haematological and serum biochemical parameters towards homeostasis.  相似文献   

15.
为了提高尖翅燕鱼 (Platax teira) 的卵孵化率和苗种成活率,并为人工催产和苗种繁育提供理论依据,观察分析了尖翅燕鱼受精卵胚胎发育和仔稚鱼发育的时序特征,对其形态学指标进行测量,推算出仔稚鱼阶段生长特性的回归方程。结果显示,尖翅燕鱼受精卵为浮性卵,直径 (1.29±0.09) mm,卵壳较坚韧,呈黄色透明的球状,单个油球,无黏性。在孵化温度 (26±0.3) ℃、盐度34~36和pH 7.8±0.5条件下,整个胚胎发育经历7个阶段共27个时期,耗时27 h 45 min。初孵仔鱼全长 (3.120±0.349) mm,呈透明状,体表散布着大量分枝叶黄素,先后经历仔鱼期 (0~17 d) 和稚鱼期 (17~25 d),25 d后进入幼鱼期。此时幼鱼鳍棘和鳍条已发育完全,其典型特征为头部、躯干部和尾部各有3条黑色条带,身体呈银白色。统计模型显示,尖翅燕鱼早期阶段生长速度较缓,7日龄后生长加速;8日龄仔鱼出现残食和攻击行为。  相似文献   

16.
掌握尖翅燕鱼 (Platax teira) 仔稚鱼脊柱及附肢骨骼系统的发育时序特征,可为其苗种繁育、环境适应及分类鉴定提供理论依据。采用软骨-硬骨双染色法对尖翅燕鱼仔稚鱼 [0~30日龄 (dah)] 脊柱、胸鳍、腹鳍、背鳍、臀鳍以及尾鳍进行染色观察与分析。结果表明,尖翅燕鱼有24枚脊椎骨,尾部骨骼由5枚尾下骨和2枚尾上骨构成,附肢支鳍骨的发育顺序为胸鳍、腹鳍、尾鳍、臀鳍和背鳍。尖翅燕鱼在初孵仔鱼已具备胸鳍支鳍骨原基和脊索,4 dah仔鱼胸鳍和腹鳍最先发育;7 dah仔鱼髓弓、脉弓开始出现;13 dah仔鱼骨骼形态和数量基本稳定;26 dah左右稚鱼开始骨化,脊柱和脊椎最先完成骨化;30 dah进入幼鱼阶段,其外表形态已经固定,与成鱼无异,典型特征为头部、躯干部和尾部各有3条黑色条带,身体呈雪白银色,受到外界刺激时体色瞬间变为黑色。尖翅燕鱼脊柱及附肢骨的发育顺序与其他海水鱼基本一致,但骨骼发育时间节点和数量有所不同。  相似文献   

17.
Transmission electron microscopy was applied to sperm removed from males and females belonging to Litopenaeus vannamei, L. stylirostris and L. occidentalis. It was discovered that a region named filamentous meshwork (FM), located between the nucleus and the hemispherical cap, develops differently in these three closely related species. In L. vannamei, the FM is synthesized in the male reproductive system, but seems to complete its formation after mating. In L. stylirostris, the FM region was not present in spermatophores collected from males or in sperm from the thelycum. In L. occidentalis, the FM region is fully developed in male sperm. It is suggested that completion of the FM is required for acrosome maturation, and the process continues after mating in some species of Litopenaeus. In vitro induction of the acrosome reaction in sperm from males and females of L. occidentalis demonstrated for the first time that reactivity is significantly superior in sperm cells that have been attached to the open thelycum for some hours, as compared to sperm in males (prior to transfer). This finding suggests that matured sperm cells of L. occidentalis become capacitated to react against egg water after mating.  相似文献   

18.
Aquaculture International - Most of the adoption studies in catfish culture were restricted to feeding habits, growth rate and morphological studies, neglecting the social factors and adoption of...  相似文献   

19.
乌鳢群体遗传多样性和遗传结构分析   总被引:1,自引:0,他引:1  
周伟  高天翔  王俊  宋娜 《水产学报》2017,41(10):1521-1532
为了解乌鳢群体遗传变异规律,本研究对8个群体共212个个体的mtDNA控制区全序列进行群体遗传多样性、遗传结构和群体历史动态分析。结果显示,乌鳢控制区全序列长度为907 bp,乌鳢群体单倍型多样性水平变化较大,中国黄河及以北的乌鳢群体单倍型多样性水平比淮河和长江等南方群体相对较低,所有群体表现出较低的核苷酸多样性水平(h0.5%)。基于单倍型构建的系统发育树和群体聚类树结果均未显示出与地理位置相对应的谱系结构。单倍型网络图显示存在多个主单倍型。遗传结构分析显示,不同水系间存在显著的遗传差异,相同水系间遗传差异较小。群体历史动态分析显示,中国乌鳢所有群体有效种群数量在中更新世晚期到晚更新世0.222—0.050百万年出现了一次较明显的快速增长,之后在晚更新世末次冰期0.050—0.010百万年出现了有效种群下降,伴随着全新世到来,在0.010百万年之后,乌鳢群体又发生了一次较小的有效种群增长。洞庭湖群体则发生一次有效种群的快速增长,增长时间大约在0.160百万年。研究表明,青藏高原隆起后,东亚季风在中国南北方气候的差异和秦岭山脉屏障对季风的阻断作用加大了这一差异,可能对乌鳢群体的遗传多样度差异造成一定影响。乌鳢群体不存在显著的谱系结构可能与乌鳢遗传分化时间较短有关,但是地理隔离等因素导致了不同水系间确切的遗传差异。第四纪更新世气候的波动,尤其是中更新世间冰期气候的转暖、末次盛冰期的降温和冰后期全新世的到来可能对乌鳢群体的数量和栖息地的扩缩起着重要影响。  相似文献   

20.
为了探讨鱼蛋白水解物对黄颡鱼生产性能的影响,以日本鳀粉为对照,以实用型黄颡鱼饲料配方模式为基础开展实验:1以30.5%鱼粉为对照(FM),在相同配方模式下,以6%鱼蛋白水解物(MPH6)替代20%的鱼粉;2以30.5%鱼粉为对照(FM),在无鱼粉日粮中分别添加3%(FPH3)、6%(FPH6)、12%(FPH12)鱼蛋白水解物;共设计5组等氮等能实验日粮,在池塘网箱中养殖黄颡鱼[初始体质量(30.08±0.35)g]60 d。结果显示:与FM相比,FPH12在SGR、FCR、PRR和FRR方面均无显著差异,而MPH6、FPH3、FPH6组SGR降低了15.45%~24.39%,FCR升高了32.14%~42.86%,MPH6、FPH6差异显著,在PRR和FRR方面,MPH6、FPH3、FPH6组PRR降低了21.11%~27.78%,MPH6组FRR降低了41.51%;全鱼水分、粗蛋白、粗脂肪和灰分各组间差异不显著,FPH3、FPH6、FPH12肌肉多种游离氨基酸水平显著高于FM,其中Thr、Val、His与其在日粮中的水平显著相关;FPH6组HSI显著低于FM,鱼蛋白水解物对CP、VSI、肠体比的影响不显著;血清AST、ALT、HDL、LDL、TP、CHOL、TG无显著差异,FPH3组ALB显著低于FM。研究表明:黄颡鱼日粮中,12%鱼蛋白水解物(干物质)与30.5%鱼粉在生长速度、饲料效率、血清生理指标等方面具有一定的等效性;过高的植物蛋白日粮影响了黄颡鱼的生产性能;饲喂鱼蛋白水解物日粮使黄颡鱼肌肉游离氨基酸的含量升高,特别是呈味氨基酸的含量增加。  相似文献   

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