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1.
Elena L. Peredo M. Ángeles Revilla Barbara M. Reed Branca Javornik Eduardo Cires José Antonio Fernández Prieto Rosa Arroyo-García 《Genetic Resources and Crop Evolution》2010,57(4):575-586
Microsatellite variation at the nuclear and chloroplast genomes was evaluated for wild European and wild American hops, in
order to assess the genetic diversity and origin of cultivated hops. Seven nuclear loci and 32 chloroplast loci were used in the analysis of 182 hop accessions including wild European (68), wild American (48), and cultivars (66). A total
of 116 alleles were identified using 7 nuclear microsatellites showing different averages of polymorphism and distribution
in the wild American and European accessions and cultivars. Two main groups were established as revealed by several statistical
analyses; one including European wild accessions and cultivars and a second group consisting of American wild accessions.
Three polymorphic chloroplast microsatellite loci were detected, six alleles were scored which defined a total of five haplotypes that were exclusive or presented different
distribution between American and European wild accessions. A major influence of the wild European haplotypes was detected
among hop cultivars. To the best of our knowledge, this is the first work reporting the use of chloroplast microsatellites
in hops. 相似文献
2.
Atsushi Murakami Peter Darby Branka Javornik Maria S. S. Pais Elisabeth Seigner Anton Lutz Petr Svoboda 《Genetic Resources and Crop Evolution》2006,53(8):1553-1562
To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite
loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci.
There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could
be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster
comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster.
Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that
hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America. 相似文献
3.
Patrizia Erre Innocenza Chessa Concepción Muñoz-Diez Angjelina Belaj Luis Rallo Isabel Trujillo 《Genetic Resources and Crop Evolution》2010,57(1):41-54
The genetic relationships within and between wild and cultivated olives were examined and clarified in an isolated and restricted
area, such as the Mediterranean island of Sardinia. Wild (21 individuals) and cultivated olive trees (22 local cultivars from
a germplasm collection and 35 ancient trees) were genotyped by means of 13 SSR loci. Five cases of synonymy were observed
and nine distinct genotypes were identified in the collection. Five novel genotypes were also detected among the ancient trees.
Differences on the allelic composition and heterozygosity levels were found between wild and cultivated trees. Model-based
clustering method classified the olive trees into two major gene pools: (a) wild genotypes and (b) local cultivars from the
collection and from heritage olives. Regarding the cultivated plant material, we observed that: (a) most of the Sardinian
cultivars shared the same allelic profiles with the ancient cultivated trees and (b) the majority of these cultivars and all
the novel genotypes were not related to any other cultivars included in this study. These findings as well as the detection
of unique alleles and a certain wild genetic background at some cultivars revealed by the Bayesian analysis may indicate their
autochthonous origin. The synonymy cases found between local cultivars and Italian mainland cultivars indicate interchange
of genetic material among these growing areas, suggesting thus a possible allochthonous origin. The information obtained can
assist in the management of an olive collection and sheds some light on the survival of true oleasters and the origin of Sardinian
cultivars. 相似文献
4.
Nahla V. Bassil B. Gilmore J. M. Oliphant K. E. Hummer J. A. Henning 《Genetic Resources and Crop Evolution》2008,55(7):959-969
Eight genic SSR loci were evaluated for genetic diversity assessment and genotype identification in Humulus lupulus L. from Europe and North America. Genetic diversity, as measured by three diversity indices, was significantly lower in European
cultivars than in North American wild accessions. Neighbor Joining cluster analysis separated the hop genotypes into European
and North American groups. These eight SSRs were useful in uniquely identifying each accession with the exception of two sets
of European landraces and a pair of Japanese cultivars, ‘Shinshuwase’ and ‘Kirin II’. An accession from Manitoba grouped with
the European (EU) cluster reflecting the group’s genetic similarity to older Manitoba germplasm used to develop ‘Brewer's
Gold’ and the gene pool arising from this cultivar. Cultivars grouped closely with one of their immediate parents. ‘Perle’
grouped with its parent ‘Northern Brewer and ‘Willamette’ grouped with its parent ‘Fuggle H’. Wild American accessions were
divided into two subgroups: a North Central group containing mostly H. lupulus var. lupuloides and a Southwestern group containing H. lupulus var. neomexicanus accessions. These eight SSRs will be valuable for genotype identification in European and wild American germplasm and may
potentially prove useful for marker-assisted selection in hop. PCR products from four previously reported primer pairs that
amplify the same intronic SSR regions as do the genic SSRs in this study were compared in eight common cultivars. Different
primer pairs generated robust markers at the chs2 and chi loci. However, only the HLC-004B and HLC-006 primer pairs amplified successfully at the chs3 and chs4 loci.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
5.
Eight genomic SSR markers with a M13 tail attached were used to assess the genetic diversity of 72 Ussurian Pear accessions
(Pyrus ussuriensis Maxim.) in China. The M13-tailed method was effective in discriminating all the 32 wild accessions. All the 40 Ussurian Pear
cultivars could be successfully discriminated with the exception of 4 sets of synonymies or spots. A total of 108 alleles
were obtained with an average of 13.5 per locus. The expected heterozygosity, observed heterozygosity, and power of discrimination
were 0.78, 0.63, and 0.86 respectively. Three triploid cultivars (‘Anli’, ‘Ruan’er’, and ‘Pitaiguo’), and one wild accession,
P. ussuriensis ‘Xilin-3’, showed three alleles at some SSRs. The number of alleles and observed heterozygosity per locus for 40 Ussurian
Pear cultivars were 9.1 and 0.62, respectively, lower than the values of 32 wild accessions which were 11.3 and 0.65, respectively.
A dendrogram based on the SSR genotypes was obtained, showing two major groups corresponding to cultivated group and wild
group. All the cultivars fell into the cultivated group. Some subgroups (Nanguoli subgroup, Zhibazi subgroup, Xiangshuili
subgroup, Balixiang subgroup, Anli subgroup) could be found in the cultivated group. A very close relationship between ‘Huagaili’
and ‘Miansuan’, and a close relationship between ‘Anli’ and a wild accession, P. ussuriensis ‘Huangshanli’ could be found in Anli subgroup. ‘Nanguoli’ and ‘Xiaowuxiang’ showed a close relationship with at least one
identical allele at each locus with the exception of NH015a. 相似文献
6.
L. Riahi N. Zoghlami K. El-Heit V. Laucou L. Le Cunff J. M. Boursiquot T. Lacombe A. Mliki A. Ghorbel P. This 《Genetic Resources and Crop Evolution》2010,57(2):255-272
Three gene pools representative of Vitis vinifera L. subsp. vinifera (=subsp. sativa Beger) growing in the Maghreb regions (North Africa) from Tunisia (44), Algeria (31) and Morocco (18) and 16 wild grape accessions
(Vitis vinifera L. subsp. sylvestris (Gmelin) Beger) from Tunisia were analysed for genetic diversity and differentiation at twenty nuclear microsatellites markers
distributed throughout the 19 grape chromosomes. 203 alleles with a mean number of 10.15 alleles per locus were observed in
a total of 109 accessions. Genetic diversities were high in all populations with values ranging from 0.6775 (Moroccan cultivars)
to 0.7254 (Tunisian cultivars). F
st pairwise values between cultivated grapevine populations were low but found to be significantly different from zero. High
F
st pairwise values were shown between wild and cultivated compartments. Two parent offspring relationships, two synonyms and
two clones of the same cultivar were detected. The rate of gene flow caused by vegetative dissemination of cultivated grapevine
plants was not sufficient to genetically homogenise the pools of cultivars grown in different regions. The Neighbour Joining
cluster analysis showed a clear separation according to geographical origins for the cultivated grapevines gene pools and
revealed a high dissimilarity between cultivated and wild grapevine. However, three cultivars (Plant d’Ouchtata 1, Plant de
Tabarka 3 and Plant d’Ouchtata 3) are very close to wild accessions and may result from a hybridisation between cultivated
and wild accessions. The high level of differentiation between cultivated and wild accessions indicates that the cultivated
accessions do not derive directly from local wild populations but could mostly correspond to imported materials introduced
from others regions during historical times or derived from crossing between them. 相似文献
7.
Naja Steen Andersen Gert Poulsen Bente Anni Andersen Lars Pødenphant Kiær Tina D’Hertefeldt Mike J. Wilkinson Rikke Bagger Jørgensen 《Genetic Resources and Crop Evolution》2009,56(2):189-200
When planning optimal conservation strategies for wild and cultivated types of a plant species, a number of influencing biological
and environmental factors should be considered from the outset. In the present study Brassica rapa was used to illustrate this: to develop Scandinavian conservation strategies for wild and cultivated B. rapa, DNA-marker analysis was performed on 15 cultivated and 17 wild accessions of B. rapa plus 8 accessions of the cross compatible B. napus. The B. rapa cultivars were bred in Sweden and Finland in 1944–1997 and the wild B. rapa material was collected from Denmark, Sweden and United Kingdom. The B. napus accessions were bred within the last 20 years in the Scandinavian countries. Results were based on scoring of 131 polymorphic
ISSR markers in the total plant material. A Bayesian Markov chain Monte Carlo (MCMC) approach implemented in NewHybrids demonstrated
a clear distinction of B. rapa and B. napus individuals except for three individuals that seemed to be backcrosses. The backcrossed hybrids descended from two Swedish
populations, one wild and one escaped. The overall pattern of genetic variation and structure in B. rapa showed that cultivated and wild B. rapa accessions formed two almost separated clusters. Geographical origin and breeding history of cultivars were reflected in
these genetic relationships. In addition, wild populations from Denmark and Sweden seemed to be closely related, except for
a Swedish population, which seemingly was an escaped cultivar. The study point to that many processes, e.g. spontaneous introgression,
naturalisation, breeding and agricultural practise affected the genetic structure of wild and cultivated B. rapa populations. 相似文献
8.
We report here that small but numerous foliar glands are present near the base of the abaxial leaf blades of hop (Humulus lupulus L.) at about 2.6 times the density found in the more distal regions, and this concentration of glands appear to protect the basal region from damage by insects. Distinctive wild taxa of H. lupulus are native to distinctive regions of the north temperate world. The much higher presence of protective glands in the three native North American Humulus taxonomic varieties in comparison with the varieties native to Europe and Asia suggests specialized leaf feeders in North America have been responsible for the evolution of greater gland density. Commercial domesticated hop cultivars trace most of their germplasm to the European hop (H. lupulus var. lupulus), which possesses a much lower concentration of leaf glands than the native hops of all other regions of the world, and therefore is likely relatively susceptible to damage. We suggest that breeding for increased density of protective foliage glands may be a non-chemical way of alleviating foliar feeding damage in cultivated hops. 相似文献
9.
Mohammad R. Sabzalian Aghafakhr Mirlohi Ghodratollah Saeidi Mohammad T. Rabbani 《Genetic Resources and Crop Evolution》2009,56(8):1057-1064
Wild species of safflower, Carthamus oxyacanthus Bieb., is highly crossable with cultivated species, C. tinctorius L. and could be directly exploited in broadening safflower gene pool and improving the crop for biotic and abiotic stress
environments. In this study, genetic diversity among accessions of C. oxyacanthus and their relationships with cultivated safflower were evaluated using agro-morphological traits and polymorphic inter-simple
sequence repeats (ISSR) markers. Significant variation was observed among accessions particularly for seeds per capitulum,
seed yield per plant, harvest index and capitula per plant. Cluster analysis based on agro-morphological traits classified
the wild accessions in two groups according to their geographical regions, and separated them from the cultivated genotypes.
ISSR marker also revealed a high genetic variation among the accessions, and cluster analysis based on this marker divided
genotypes into four groups, with cultivated ones in a separate clade. Genetic variation observed among the wild safflower
germplasm at the DNA level was higher than the agro-morphological traits, indicating that ISSR is an effective marker system
for detecting diversity among safflower genotypes and their genetic relationships. Accessions of C. oxyacanthus with high genetic relationship to cultivated species could be used for interspecific hybridization in breeding programs of
safflower. 相似文献
10.
S. Boucheffa M. M. Miazzi V. di Rienzo G. Mangini V. Fanelli A. Tamendjari D. Pignone C. Montemurro 《Genetic Resources and Crop Evolution》2017,64(2):379-390
The present work was aimed at assessing the genetic diversity of 42 local cultivars and oleaster genotypes from the area of Bejaia in Algeria. Fifteen highly polymorphic Simple Sequence Repeat markers were evaluated and proved to be very informative, producing a total number of 160 alleles with an average value of 10.7 per locus; the SSRs DCA09 and DCA16 were the most informative, distinguishing 17 and 19 genotypes, respectively. Phylogenetic and population structure analysis split the accessions in two main groups corresponding to most of oleasters and most of traditional varieties, respectively. Interestingly, ten traditional varieties resulted strictly related to the oleasters, indicating hybridization between the two botanical varieties. Genetic parameters and private alleles of groups confirmed this observation and indicated a wide genetic variability in Algerian olive germplasm. The results suggest the need to preserve and characterize this germplasm in order to limit the risk of losing potential important genetic traits present in the crop wild relatives. 相似文献
11.
In the present study genetic diversity and hybridization with cultivars were investigated in a population of the endangered European wild apple species Malus sylvestris (L.) Mill. with the aim to establish a basis for the implementation of conservation activities and to ensure its long-term preservation. A total of 284 putative M. sylvestris trees located in the East Ore Mountains were investigated along with a standard set of reference apple genotypes proposed by the European Cooperative Program for Plant Genetic Resources (ECPGR) and 13 old apple cultivars often cultivated in Saxony. The genetic analysis was performed using 12 microsatellite markers also recommend by the ECPGR. To differentiate ‘true type’ M. sylvestris individuals, hybrids and apple cultivars (Malus × domestica Borkh.) a model-based cluster analysis was performed using STRUCTURE. Two clusters were identified consisting of M. sylvestris and M. × domestica genotypes. About 40 % of the putative M. sylvestris showed an admixture of the species-specific allele frequencies and were defined as hybrids. The genetic diversity of the ‘true type’ M. sylvestris population was still high but slightly lower than in the apple cultivars especially since some SSR loci were fixed on one or few alleles in the M. sylvestris population. The differentiation parameters between ‘true type’ wild apple and cultivars indicated a clear discrimination between the wild and cultivated apple individuals. This fact confirms our expectation of the existence of ‘true type’ M. sylvestris individuals in the East Ore Mountains and argues for the realization of preservation measures in this area. 相似文献
12.
Michael Dossett Nahla V. Bassil Kim S. Lewers Chad E. Finn 《Genetic Resources and Crop Evolution》2012,59(8):1849-1865
Breeding progress in black raspberry (Rubus occidentalis L.) has been limited by a lack of genetic diversity in elite germplasm. Black raspberry cultivars have been noted for showing very few phenotypic differences and seedlings from crosses between cultivars for a lack of segregation for important traits. Despite these challenges, little molecular work has been done to explore genetic diversity and relationships in wild and cultivated black raspberry germplasm. Microsatellite, or simple sequence repeat (SSR), markers are highly polymorphic codominant markers useful for studying genetic diversity, population genetics, genetic fingerprinting and other applications. We examined genetic diversity in 148 wild and cultivated black raspberry accessions using 21 polymorphic SSR markers. Black raspberry cultivars clustered tightly and showed higher than expected heterozygosity while that of wild accessions was low. Relationships between wild black raspberry accessions were poorly resolved and regional clusters were mostly absent from our analysis. Our results indicated that wild black raspberry germplasm is a relatively untapped resource available for future breeding. 相似文献
13.
Y. Shakhatreh N. Haddad M. Alrababah S. Grando S. Ceccarelli 《Genetic Resources and Crop Evolution》2010,57(1):131-146
Wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch) Thell., is the progenitor of cultivated barley. Almost unanimously the center of diversity is considered to be
in the Fertile Crescent of the Near East, where wild barley grows under a wide range of environmental and climatic conditions.
Jordanian wild barley is expected to harbor genes useful for the improvement of cultivated barley, particularly those associated
with tolerance to drought. This study evaluated 103 wild barley accessions collected from different areas of Jordan along
with 29 cultivated barley genotypes for several morphological and agronomical traits. The Hordeum vulgare ssp. spontaneum C. Koch accessions were grouped into six populations according to the longitude, latitude, altitude, and rainfall zone of
the collection site, and the cultivated barley in one population. The evaluation was conducted during the 2004–2005 growing
season under field conditions in three locations in Jordan; namely, Khanasri, Ramtha, and Maru with 123.0, 222.9, and 429.2 mm
annual rainfall, respectively. We used an unreplicated design with two systematic checks (the cultivars Rum and Mu’ta) each
repeated 15 times. The results showed the existence of high variability among the Hordeum vulgare ssp. spontaneum C. Koch accessions for most of the traits, especially for plant height, tiller number, days to heading, days to anthesis,
peduncle length, and peduncle extrusion. Plant height, earliness, peduncle length, and peduncle extrusion were found to be
adaptive traits under drought conditions and several superior genotypes for each trait were identified. Genetic variation
within population was much higher than between populations. Clustering of populations was according to their ecological geographical
pattern. 相似文献
14.
Yosuke Kuroda Norihiko Tomooka Akito Kaga S. M. S. W. Wanigadeva Duncan A. Vaughan 《Genetic Resources and Crop Evolution》2009,56(8):1045-1055
Wild soybeans, Glycine soja, are a source of genetic variation to improve soybeans. To improve the efficiency evaluation of conserved germplasm a core
or mini-core collection approach that maximizes allelic diversity in a proportion of the whole collection has frequently been
advocated. The genetic diversity of a wild soybean collection (1,305 accessions) plus Japanese cultivated soybeans (53 accessions)
were analyzed at 20 SSR marker loci. Higher levels of allelic diversity were found in wild soybeans (28 alleles per locus)
than Japanese cultivated soybean (five alleles per locus). The genetic distance between wild soybeans from different regions
reflected their proximity. Accessions from Russia consisted of a diverse array of alleles resulting in accessions being spread
further apart in a PCA plot than accessions from other regions. Accessions of wild soybean from Korea included many rare alleles
and thus had a high representation in the core collection. The two core collections developed here, traditional and mini,
consisted of 192 accessions with 97% of the allelic diversity (14% of the whole collection) and 53 accessions with 62.4% of
the allelic diversity (5% of the whole collection), respectively. 相似文献
15.
Stina Christensen Roland von Bothmer Gert Poulsen Lorenzo Maggioni Marianne Phillip Bente Anni Andersen Rikke Bagger Jørgensen 《Genetic Resources and Crop Evolution》2011,58(5):657-666
AFLP markers were used to characterize diversity and asses the genetic structure among 17 accessions of kale landraces, cultivars
and wild populations from Europe. The range of average gene diversity in accessions was 0.11–0.27. Several landraces showed
higher levels of diversity than the wild populations and one cultivar had the lowest diversity measures. The landraces that
were most genetically diverse were from areas where kales are known to be extensively grown, suggesting in situ conservation in these areas as a supplement to storage of seeds in gene banks. An analysis of molecular variance (AMOVA)
showed that 62% of the total variation was found within accessions. For most accessions, genetic distance was not related
to geographic distance. Similarities among accessions were probably not caused by recent gene flow since they were widely
separated geographically; more likely the relationship among them is due to seed dispersal through human interactions. Our
results indicate that a kale population found in a natural habitat in Denmark was probably not truly wild but most likely
an escape from a cultivated Danish kale that had subsequently become naturalized. 相似文献
16.
Ali Gharghani Zabihollah Zamani Alireza Talaie Nnadozie C. Oraguzie Reza Fatahi Hassan Hajnajari Claudia Wiedow Susan E. Gardiner 《Genetic Resources and Crop Evolution》2009,56(6):829-842
In order to shed light on the role of Iran in apple evolution and domestication, we chose to investigate the relationships
of a collection of 159 accessions of wild and domesticated apples including Iranian indigenous apple cultivars and landraces,
selected wild species, and old apple scion and rootstock cultivars from different parts of the world. The majority of the
wild species belonged to M. sieversii, which is widely believed to be the main maternal wild ancestor of domestic apples, from Kazakhstan and M. orientalis, which is one of the probable minor ancestors of domestic apples, from Turkey and Russia located on the east and west of
Iran, respectively. The accessions were assigned into six arbitrary populations for the purpose of generating information
on genetic parameters. Nine simple sequence repeat (SSR) loci selected from previous studies in apple were screened over DNA
extracted from all the accessions. Results showed that all SSR loci displayed a very high degree of polymorphism with 11–25
alleles per locus. In total, there were 153 alleles across all loci with an average of 17 alleles per locus. The SSR allelic
data were then used for estimation of population genetic parameters, including genetic variation statistics, F-statistics,
gene flow, genetic identity, genetic distance and then cluster analysis using POPGENE 1.32 software. The F-statistics and
gene flow in particular, showed that there was more intra-population than between population variation. The genetic identity
and genetic distance estimates, and the dendrogram generated from the un-weighted pair group arithmetic average (UPGMA) method
of cluster analysis showed that the Iranian cultivars and landraces were more closely related to M. sieversii from Central Asia (east of Iran) and M. orientalis native to Turkey and Russia than to other accessions of Malus species. Also, the old apple cultivars from different parts of the world have a closer genetic relationship to M. sieversii, M. orientalis and the Iranian apples, than to other wild species. Based on these results, we suggest that the Iranian apples may occupy
an intermediate position between the domesticated varieties and wild species. We propose that Iran could be one of the major
players in apples’ domestication and transfer from Central Asia to the western countries. 相似文献
17.
Kishimoto T Kobayashi M Yako N Iida A Wanikawa A 《Journal of agricultural and food chemistry》2008,56(3):1051-1057
Contributions of hop-derived thiols were examined. Extremely strong fruity, black currant-like aromas were detected in beers hopped with some U.S. cultivars. 4-Mercapto-4-methylpentan-2-one (4MMP) was supposed to be the main contributor to the fruity aroma, and the contents between cultivars were investigated. In hop pellets, a negative correlation between 4MMP concentration and copper ion content in hops was observed. 4MMP was detected only in U.S., Australian, and New Zealand cultivars, but no European ones, which are treated with copper-containing fungicides (Bordeaux mixture) and therefore have a high content of copper ions. The 4MMP content was highest in Simcoe cultivars, followed by Summit, Apollo, Topaz, Cascade pellets, and also differed between crop years. It was indicated that most 4MMP exists freely in wort or in hop pellets with only small amounts formed from precursors and that the amounts increased during the fermentation process. 相似文献
18.
Genetic diversity in 79 European accessions of the Barley Core Collections was surveyed using isozyme electrophoresis. A total of 26 alleles were observed at the ten isozyme loci. All loci were polymorphic except Pgd-1 which was monomorphic. The comparison of the results with those of previous studies indicates that most of the alleles occurring in the European Barley are also observed in this set of the European Barley Core Collections. Only five alleles (Est-1 Al; Est-5 Ag, Te; Pgi-1 C and Ndh-2 B) were absent. Nine of 26 alleles were rare alleles, which were detected only in one or two accessions. Moreover, most of rare alleles were detected in 6-rowed winter barley. It is very important to include rare alleles for maximising the genetic variations in core collections. In the set of European Barley Core Collection, 6-rowed barley contained larger diversity than 2-rowed barley; winter type contained larger diversity than spring type. The cluster analysis separated 79 accessions into three major groups. Group I is more complex and comprised 2-rowed spring, 2-rowed winter and 6-rowed winter barley. In this group, 18 accessions in the cluster A and 14 accessions in the cluster B possessed identical genotypes as judged from the ten isozyme data. Principal coordinate analysis could not clearly separate the spring cultivars from the winter barley lines, as well as not separate 2-rowed from 6-rowed barley. 相似文献
19.
To evaluate the genetic diversity and to clarify the genetic relationships of Japanese peach cultivars, we analyzed the amplified
fragment length polymorphism (AFLP) and traced the pedigree of 17 Japanese commercial peach cultivars and six traditional
accessions. Sixteen AFLP primer combinations produced a total of 837 fragments and 146 polymorphic bands with a polymorphism
percentage of 17.5%. All of the peach accessions could be identified from differences in at least 10 polymorphic bands. A
cluster analysis showed that all the Japanese commercial peach cultivars, except ‘Kiyomi’ and ‘Jichigetsuto’, formed a major
group consisting of three sub-groups. Of the six traditional accessions, four were genetically distant from the Japanese commercial
peach cultivars while two accessions from China were classified into the Japanese commercial peach cultivars group. Both the
AFLP analysis and pedigree tracing suggested that Japanese commercial peach cultivars are mainly derived from ‘Shanhai Suimitsuto’,
one of the traditional accessions from China. Although the genetic relationships revealed by AFLP were generally in agreement
with those shown by the pedigree information, some contradictions were found. Combining the AFLP results and pedigree information
can provide a better understanding of the genetic relationships of Japanese peach cultivars. 相似文献
20.
Łukasz Wolko Wojciech Antkowiak Elżbieta Lenartowicz Jan Bocianowski 《Genetic Resources and Crop Evolution》2010,57(6):801-806
The aim of this study was to identify the group of highly polymorphic microsatellite markers for the identification of six
pear cultivars (P. communis) and two individuals of wild pear (P. pyraster). From among 40 tested SSR markers, 19 were selected to profile genetic diversity in pear genotypes due to high polymorphisms.
These markers showed high heterozygosity levels (0.5–1) and, on average, 6.4 alleles per marker were found. The set of microsatellite
markers employed in this study demonstrated usefulness of microsatellite markers for the identification of pear genotypes.
The examined wild forms were represented in this study by only two individuals of P. pyraster. It can be assumed that these forms were distinctly different from the cultivated pear cultivars. 相似文献