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1.
Although common bean (Phaseolus vulgaris L.) is cultivated throughout India, the Himalayas hold largest diversity of bean germplasm. No studies on characterization
of phaseolin types on this germplasm have been conducted earlier. In order to determine whether the common bean cultivars
collected from various areas in the Northern Himalayas represent introductions from the Central American and Andean domestication
centers or are local domesticates, we have analyzed the electrophoretic variation (SDS-PAGE) of phaseolin types in several
bean accessions. A few species of Vigna were also included in this study to determine whether phaseolin (vignin in Vigna) patterns can be used to resolve the Phaseolus–Vigna complex.
The present investigation on phaseolin (globulin) patterns of Phaseolus vulgaris and Vigna spp. clearly shows much variability in globulin patterns. Three new types of phaseolin patterns were recorded. An attempt
to resolve phylogenetic problems in this complex was made using the phaseolin data. 相似文献
2.
Common bean (Phaseolus vulgaris L.) was introduced to East Africa over 400 years ago and is today a fundamental part of food and income security of many smallholder farmers in the region. East Africa is among the top three bean producing and consuming regions of Africa with Uganda, Rwanda and Kenya being leaders in production. The leading common bean producing region in Uganda is the Southwestern (SW) highlands. Production is subsistent and farmers grow mixed varieties (accessions) to minimize losses. The aim of this study was to investigate the impact of farmer selection and production practices on genetic diversity of common bean in SW Uganda. 100 accessions were assembled from SW Uganda and assayed with 6 DNA simple sequence repeat (SSR) markers. A total of 41 alleles were detected giving an overall average gene diversity of 0.299 (30%) in both districts. The accessions clustered into two major gene pools i.e., Mesoamerican and Andean. Within each gene pool there was evidence of clonal populations suggesting wide distribution of certain accessions. Kabale district had a higher average gene diversity (38%) compared to Kisoro district (22%). Detection of Andean phaseolin in a clone set of Mesoamerican gene pool suggests introgession between the two groups. 相似文献
3.
Angela R. Piergiovanni Domenico Cerbino Michele Brandi 《Genetic Resources and Crop Evolution》2000,47(5):489-495
The variation within a collection constituted by 36 populations of common bean (Phaseolus vulgarisL.) recently collected in Basilicata region (Southern Italy) was studied. These populations are cultivated in marginal areas of the region mainly for farms' self-consumption. An appreciable variation of seed shape, colour and type of pattern was observed; 34 populations are of climbing growth habit. The frequency of the phaseolin types within the collection was investigated. C, T and S phaseolin types were observed, the type C being predominant (18 of 36 landraces); intra-population variation was detected only for two landraces. Local populations were compared with 28 commercial cultivars widely grown in the Basilicata region and accounting for 80–90% of common bean production. Significant divergences in growth habit and frequency of phaseolin types were observed: 22 cultivars had bush growth habit and 20 showed T phaseolin type. These results stress the urgency of actions devoted to the safeguard of this local germplasm. In fact, the diffusion of alloctonous and not typical germplasm is the first step towards the erosion of useful or valuable genes present in those established local populations. 相似文献
4.
The aim of present study was to know about past cultivation of pseudocereals and millets in Kashmir province of Indian state of Jammu and Kashmir. These crops which were cultivated throughout Kashmir in plains as well as in hilly areas are buckwheat species, Fagopyrum esculentum and Fagopyrum tataricum; amaranth and minor millets, Setaria italica and Panicum miliaceum. In Kashmir these are now forgotten crops. Few plants of amaranth (Amaranthus caudatus) are still grown in almost every kitchen garden in Kashmir. The crop is however not cultivated commercially neither in Kashmir nor in Ladakh or Jammu provinces of the state. Our study has revealed that cultivation of all these crops has been abandoned some five or six decades back throughout Kashmir excepting in few farflung hilly areas in north Kashmir. Currently these crops are also cultivated in Ladakh, but here also their cultivation is dwindling rapidly. The reasons as to why farmers have abandoned these crops and are increasingly losing interest in these biological assets in the areas of their present cultivation have been discussed. There is a need to rescue these threatened crops which have many advantages besides, being nowadays categorized as quality health foods. During our survey in Ladakh province we have collected 30 germplasm accessions of these crops from various locations. These have been deposited in National Seed Gene Bank at NBPGR, New Delhi for conservation and evaluation. The improvement and revival of these lost crops has the potential to diversify agriculture, food system and income of the poor farmers and have the ability to ensure food security and livelihood of the people in this Himalayan state under future adverse climatic conditions. 相似文献
5.
Therése Bengtsson The PPP Barley Consortium Outi Manninen Ahmed Jahoor Jihad Orabi 《Genetic Resources and Crop Evolution》2017,64(8):2021-2033
Genetic diversity, population structure and genome-wide linkage disequilibrium (LD) was estimated in Nordic spring barley (Hordeum vulgare L. subsp. vulgare) by genotyping 180 breeding lines with 48 SSR markers and 7842 high-confidence SNPs using the Illumina Infinium 9K assay. In total 6208 SNPs were polymorphic and selected for further statistical analysis. A Mantel test revealed a strong positive correlation with a Pearson’s correlation coefficient (r) of 0.86, between the estimates of genetic distances based on SSR and SNP data. Population structure analysis identified two groups with a clear ancestry and one group with an admixed ancestry. The groups were primarily separated based on row-type and geographical origin. Average LD for the whole population decayed below a critical level of r2 = 0.20 within a range of 0–4 cM. To avoid confounding effects of the strong population structure, LD decay for the different groups was analysed separately and ranged from 0 to 12 cM. A slower LD decay was found within the two-rowed lines compared to the six-rowed lines and the two-rowed lines originating from the northern part, which could be the result of strong selection for malting quality and yield in the southern part. No large difference in genetic diversity was observed between population sub-groups, but differences at certain chromosomal regions were evident. 相似文献
6.
Montoya CA Leterme P Victoria NF Toro O Souffrant WB Beebe S Lallès JP 《Journal of agricultural and food chemistry》2008,56(6):2183-2191
A study was conducted to investigate the amino acid (AA) composition and the susceptibility to in vitro proteolysis (pepsin, 120 min and pancreatin, 240 min) of a collection of purified phaseolins ( n = 43) in unheated or heat-treated form. The AA composition of phaseolin varied little across bean varieties. At 360 min of in vitro proteolysis, the degree of hydrolysis varied from 11 to 27% for unheated and from 57 to 96% for heated phaseolins ( P < 0.001). Heat treatment markedly increased the susceptibility of phaseolin to proteolysis ( P < 0.001). The AA scores (AAS) and the protein digestibility corrected for AAS indicated S-containing AA as the limiting AA (39 +/- 3 and 30 +/- 5%, respectively). In conclusion, susceptibility to proteolysis of heat-treated phaseolin rather than its AA composition affects the nutritional value of phaseolin estimated in vitro. Therefore, it should be the criterion of choice in breeding programs aimed at improving the nutritional value of common beans for humans. 相似文献
7.
A. Lázaro B. Villar L. Aceituno-Mata J. Tardío L. De la Rosa 《Genetic Resources and Crop Evolution》2013,60(5):1641-1654
At the mountain area close to the city of Madrid, common beans were one of the main food crops present in everyday diet until 1960. This paper describes the morphological diversity, for forty-six phenological and morphological traits, found in forty-three traditional varieties of common beans collected in this area and seven commercial varieties used as reference. That comparison suggests that common bean breeding programs have led to later varieties with a higher production of straight pods. Quantitative traits showed also similar values when compared to the Iberian common bean collection, except for the phenological and seed size characters. The phenological differences could suggest an ecological adaptation of the studied landraces to the regional environmental conditions. The different seed size averages of both collections might correspond to the scarce presence of smaller seed-type common beans in Madrid collection. All the accessions collected in the Sierra Norte of Madrid belong to any of the groups included in the Spanish core collection. Madrilenian collection is also composed by indeterminate growth habit varieties, while the presence of bush accessions is relatively uncommon. Most of the Madrilenian landraces have a remarkable fitness for green-pod consumption (42 %) and their seeds are mainly white (30 %), ovate-shape (49 %) and medium-large (40 %). The study of seed storage protein allowed to classify the landraces according to their domesticated gene pools. Most of them (72 %), with T and C phaseolin type, seem to belong to Andean germplasm, while the remaining (28 %), with S and B phaseolin type, to Mesoamerican one. The remarkable morphological diversity of common beans found in this small area is a symptom of a broad genetic base despite genetic erosion, what indicates a widespread crop in the past. Therefore, it is advisable to design agro-environmental policies to promote the production and commercialization of common bean landraces in Sierra Norte of Madrid. 相似文献
8.
A. Séraphin Zanklan Heiko C. Becker Marten Sørensen Elke Pawelzik Wolfgang J. Grüneberg 《Genetic Resources and Crop Evolution》2018,65(3):811-843
Yam bean [Pachyrhizus DC.] is a legume genus of the subtribe Glycininae with three root crop species [P. erosus (L.) Urban, P. tuberosus (Lam.) Spreng., and P. ahipa (Wedd.) Parodi]. Two of the four cultivar groups found in P. tuberosus were studied: the roots of ‘Ashipa’ cultivars with low root dry matter (DM) content similar to P. erosus and P. ahipa are traditionally consumed raw as fruits, whereas ‘Chuin’ cultivars with high root DM content are cooked and consumed like manioc roots. Interspecific hybrids between yam bean species are generally completely fertile. This study examines the genetic diversity of the three crop species, their potentials for breeding and the identification of useful traits to differentiate among yam bean genotypes and accessions. In total, 34 entries (genotypes and accessions) were grown during 2000?2001 at two locations in Benin, West Africa, and 75 morphological and agronomical traits, encompassing 50 quantitative and 25 qualitative characters were measured. Diversity between entries was analyzed using principal component analysis, cluster analysis, multivariate analysis of variance and discriminant function analysis. Furthermore, phenotypic variation within and among species was investigated. Intra- and interspecific phenotypic diversity was quantified using the Shannon–Weaver diversity index. A character discard was tested by variance component estimations and multiple regression analysis. Quantitative trait variation ranged from 0.81 (for total harvest index) to 49.35% (for no. of storage roots per plant). Interspecific phenotypic variation was higher than intraspecific for quantitative traits in contrast to qualitative characters. Phenotypic variation was higher in overall for quantitative than qualitative traits. In general, intraspecific phenotypic variation ranged from 0.00 to 82.61%, and from 0.00 to 80.03% for quantitative and qualitative traits, respectively. Interspecific phenotypic variation ranged from 0.00 to 95.02%, and 0.00?81.58% for the two trait types, respectively. The Shannon–Weaver diversity index (H′) was in general high and over 0.80 for most of the trait. Diversity within P. tuberosus was higher than within P. erosus and P. ahipa. Across the 50 quantitative and 25 qualitative traits, the Shannon–Weaver diversity index of intra- and interspecific variation was around 0.83 and 0.51, respectively and was lower for qualitative than for quantitative traits. Monomorphism was observed in eight qualitative traits and one quantitative character. The first, second and third principal components explained, respectively, 39.1, 21.3 and 8.3% of the total variation in all traits. Pachyrhizus erosus, P. ahipa, and P. tuberosus (‘Chuin’ and ‘Ashipa’) were clearly separated from each other by these analyses. Multivariate analysis of variance indicates significant differences between Pachyrhizus species for all individual or grouped traits. Discriminant function analysis revealed that the first two discriminant functions were almost significant. Biases due to unbalanced sample size used per species were small. Within each species a similar amount of diversity was observed and was determinable to 70% by only ten traits. We conclude that the cultivated yam bean species represent distinct genepools and each exhibits similarly large amounts of genetic diversity. 相似文献
9.
Giuseppina Logozzo Rosa Donnoli Leonardo Macaluso Roberto Papa Helmut Knüpffer Pierluigi Spagnoletti Zeuli 《Genetic Resources and Crop Evolution》2007,54(8):1763-1779
Common bean (Phaseolus vulgaris L.) was introduced in Europe from both Mesoamerican and Andean centres of origin. In this study, a collection including 544
accessions from all European regions showed that the Andean phaseolin types ‘T’ (45.6%) and ‘C’ (30.7%) prevailed over the
Mesoamerican ones ‘S’ (23.7%), and accessions with cuboid seed shape (34.9%), maroon coat darker colour seed (44.3%), uniform
seed colour (69.6%) were the most frequent. European accessions with phaseolin ‘S’ showed a significantly larger average seed
size compared to those from America in the same phaseolin class while those presenting ‘T’ and ‘C’ phaseolin did not. This
suggests that, during crop expansion in Europe, sampling or selection favoured the large-seeded races within the Mesoamerican
‘S’ gene pool or, possibly, introgression from Andean germplasm did occur. A core collection was developed using sampling
approaches based on the information available in the genebank databases and on phaseolin patterns. Four sampling strategies
were used: simple random sampling, and three random-stratified samplings, by logarithm of frequency of accessions by country,
by European region, and by phaseolin pattern, respectively. Two sampling strategies resulted in core collections significantly
different for phaseolin electrophoretic patterns from the whole collection. Stratification by phaseolin patterns increased
the frequency of ‘S’ types (‘C’ type = 33%, ‘T’ type = 5.7% and ‘S’ type = 31.3%). The core collections were validated using
seven seed characters, and no significant difference was observed in all strategies. This first developed European bean core
collection will help to assess the contribution of the two American gene pools to the European germplasm and their relative
importance for breeding purposes. 相似文献
10.
A.P. Rodiño M. Santalla I. Montero P.A. Casquero A.M. De Ron 《Genetic Resources and Crop Evolution》2001,48(4):409-417
Common bean (Phaseolus vulgarisL.) is a traditional crop in Portugal, where farmers growvarieties selected and maintained by themselves. A collection of 88landraces of common bean was evaluated for 17 quantitative andqualitative traits and the biochemical marker phaseolin to displaythe degree of variation of this germplasm. Agronomic data weresubjected to cluster analysis and several groups were identified,with three groups clustering most of the landraces. Regardingphaseolin variation the C and T banding patterns are the mostfrequent ones, so the origin of the Portuguese beans is thus probablythe Andean region of South America. These results give informationabout the origin, diversity and breeding value of the Portuguesegermplasm, that could be useful to widen the genetic base ofcurrently cultivated bean varieties in Europe. 相似文献
11.
‘Ladakhi tea’ <Emphasis Type="Italic">Bidens pilosa</Emphasis> L. (Asteraceae): a cultivated species in the cold desert of Ladakh Himalaya,India 总被引:1,自引:1,他引:0
Bidens pilosa L., a less-known cultivated tea species was collected from cold desert of Ladakh Himalaya, Jammu and Kashmir, India during
2004. The freshly harvested leaves are used in preparation of ‘Ladakhi tea’ locally known as ‘Saja’ or ‘Soljaa’ in cold desert
of Ladakh Himalaya, Western Himalaya, Jammu and Kashmir. The local inhabitants were growing this species in their kitchen
garden for this use. In the present communication, the method of preparation of this beverage is also discussed. 相似文献
12.
Population genetic studies are effective ways of researching the origin of, and genetic variation within, crop species, with a view to breeding for increased tolerances or novel traits. This is particularly important now that we are facing climate change and an increasing global population. Lablab purpureus (L.) Sweet (hyacinth bean) is an underutilised legume that has the potential of being an important crop species in the future due to its enhanced environmental tolerances relative to other legumes. It is farmed extensively, but locally, throughout Africa and Asia, however limited research and development of the crop has been undertaken so far, hence an investigation into its origin and diversity is warranted. Our microsatellite analysis suggests an East African origin of Lablab because of the genetic similarities between East African lines and the wild subspecies, subsp. uncinatus. The East African lines were also more genetically diverse. Two chloroplast DNA haplotypes were resolved and Africa was the only continent where both were present, again suggesting an African origin followed by the dissemination of lines outside of Africa coupled with a reduction in genetic diversity. Variation in tolerance to drought was recorded, with some lines able to tolerate 14 days without watering. In sum, we propose an East African origin of Lablab and have identified potential adaptive diversity for future crop breeding attempts. 相似文献
13.
Worku Negash Mhiret J. S. Heslop-Harrison 《Genetic Resources and Crop Evolution》2018,65(6):1603-1614
Molecular characterization of germplasm is important for sustainable exploitation of crops. DNA diversity was measured using inter-retrotransposon-amplified-polymorphism and inter-simple-sequence-repeat markers in 203 Ethiopian landraces and reference varieties of linseed (flax, Linum usitatissimum) and wild Linum species. Molecular diversity was high (PIC, 0.16; GD, 0.19) compared to other reports from the species. Genotyping separated reference from landrace accessions, and clustered landrace accessions from different altitudes and geographical regions. Collections showed evidence for recent introduction of varieties in some regions. The phylogeny supported L. bienne Mill. as the progenitor of domesticated L. usitatissimum. Markers developed here will be useful for genetic mapping and selection of breeding lines. The results show the range of characters that can be exploited in breeding lines appropriate for smallholder and commercial farmers in Ethiopia, producing a sustainable, secure, high-value crop meeting agricultural, economic and cultural needs. 相似文献
14.
J. S. Penha A. C. A. Lopes R. L. F. Gomes J. B. Pinheiro J. R. Assunção Filho E. A. Silvestre J. P. G. Viana J. Martínez-Castillo 《Genetic Resources and Crop Evolution》2017,64(6):1355-1364
Lima bean (Phaseolus lunatus L.) is an important food source in Brazil, especially in the northeast region, where its production and consumption are high. The goals of the present study were to estimate natural outcrossing rates and genetic diversity levels of Lima bean from Brazil, using ten microsatellite loci to obtain information for their conservation and breeding. Fourteen accessions were selected from an experiment in field with open-pollinated and with the presence of pollinating insects. Twelve seeds of each of the 14 selected accessions were grown in screenhouse for tissue harvest and DNA extraction. The multilocus model was used to determine the reproductive system. The outcrossing rate was 38.1 % (tm = 0.381; ts = 0.078), and the results indicated a mixed mating system with a predominance of selfing (1 ? tm = 61.9 %). The biparental inbreeding rate was high (t m ? t s = 0.303) and the multilocus correlated paternity was quite high (r p(m) = 0.889), indicating that the progeny was mostly composed of full sibs. The average effective number of pollen donors per maternal plant (N ep ) was low (1.12), and the fixation index for maternal genotypes (F m ) was 0.945, indicating that most genitors resulted from inbreeding. The studied families presented considerable genetic variability: A = 6.10; %P = 30; H e = 0.60 and H o = 0.077. Total diversity was high (H T = 0.596), and a portion was distributed within families (H S = 0.058). In addition, diversity was higher between families (D ST = 0.538), and genetic differentiation was high (G ST = 0.902). The results presented here can be used in the implementation of Lima bean conservation and breeding programs in Brazil. 相似文献
15.
Stephan Schröder Bochra A. Bahri Douglas M. Eudy Daniel J. Layton Elizabeth A. Kellogg Katrien M. Devos 《Genetic Resources and Crop Evolution》2017,64(2):367-378
Setaria viridis (L.) P. Beauv. and its domesticated form, S. italica (L.) P. Beauv., have been developed over the past few years as model systems for C4 photosynthesis and for the analysis of bioenergy traits. S. viridis is native to Eurasia, but is now a ubiquitous weed. An analysis of the population structure of a set of 232 S. viridis lines, mostly from North America but also comprising some accessions from around the world, using 11 SSR markers, showed that S. viridis populations in the US largely separate by latitude and/or climatic zone. S. viridis populations from the Northern US and Canada (north of 44°N) group with accessions from Western Europe, while populations in the Mid and Southern US predominantly group with accessions from Turkey and Iran. We hypothesize that S. viridis in the US was most likely introduced from Europe, and that introductions were competitive only in regions that had climatic conditions that were similar to those in the regions of origins. This hypothesis is supported by the fact that Canadian S. viridis lines were fast cycling and undersized when grown in the Mid-Western and Southern US compared to their morphology in their native environment. A comparison of the population structure obtained with 11 SSR markers and ~40,000 single nucleotide polymorphisms (SNPs) in a common set of S. viridis germplasm showed that both methods essentially yielded the same groupings, although admixture was identified at a higher frequency in the SNP analysis. Small numbers of SSR markers can thus be used effectively to discern the population structure in this inbreeding species. 相似文献
16.
Luciana Camacho-Pérez Jaime Martínez-Castillo Javier O. Mijangos-Cortés Miriam M. Ferrer-Ortega Jean Pierre Baudoin Rubén H. Andueza-Noh 《Genetic Resources and Crop Evolution》2018,65(1):229-241
Lima bean (Phaseolus lunatus L.) is an important crop in the Mayan culture. The Mayan area, considered as a main center of Mesoamerican diversity, has been divided into two subareas: the Mayan lowlands and the Mayan highlands. The Yucatan Peninsula is part of the Mayan lowlands and holds the highest number of Lima bean landraces of Mexico, but Lima beans are in high risk of genetic erosion due to intensification of the traditional Mayan agriculture. However, information on genetic diversity of Lima beans of the Mayan highlands is lacking. By using 46 landraces collected in the Mayan area (23 from each subarea) and 73 ISSR loci (inter-simple sequence repeats), we analyzed the structure, diversity and genetic relationships of Lima beans of this part of Mesoamerica. High levels of diversity (H BAY = 0.45) and genetic structure (F ST = 0.66) were found for the whole Mayan area. Genetic diversity in the Mayan lowlands was apparently higher than the Mayan highlands (H BAY = 0.44 and 0.36, respectively); but differences were not statistically significant. Genetic structure between the subareas was high (AMOVA = 30% of total variation), most landraces grouping according to their geographic origin. This study shows the importance of the Mayan culture in the diversification and conservation of Lima beans. The results provide important information that should be considered when implementing strategies to collect Lima bean landraces and planning in situ and ex situ programs to conserve these landraces in the Mayan region. 相似文献
17.
F.M.A. Islam K.E. Basford R.J. Redden A.V. Gonzalez P.M. Kroonenberg S. Beebe 《Genetic Resources and Crop Evolution》2002,49(3):271-283
It is generally accepted that two major gene pools exist in cultivatedcommon bean (Phaseolus vulgaris L.), a MiddleAmerican and an Andean one. Some evidence, based on unique phaseolin morphotypesand AFLP analysis, suggests that at least one more gene pool exists incultivated common bean. To investigate this hypothesis, 1072 accessions from acommon bean core collection from the primary centres of origin, held at CIAT,were investigated. Various agronomic and morphological attributes (14categorical and 11 quantitative) were measured. Multivariate analyses,consisting of homogeneity analysis and clustering for categorical data,clustering and ordination techniques for quantitative data and nonlinearprincipal component analysis for mixed data, were undertaken. The results ofmost analyses supported the existence of the two major gene pools. However, theanalysis of categorical data of protein types showed an additional minor genepool. The minor gene pool is designated North Andean and includes phaseolintypes CH, S and T; lectin types 312, Pr, B and K; and mostly A5, A6 and A4 types-amylase inhibitor. Analysis of the combined categorical data ofprotein types and some plant categorical data also suggested that some othergermplasm with C type phaseolin are distinguished from the major gene pools. 相似文献
18.
Arun Kumar M. K. Kaul M. K. Bhan Punit K. Khanna K. A. Suri 《Genetic Resources and Crop Evolution》2007,54(3):655-660
Six phenotypic characters and three withanolide markers were assessed in 25 accessions of Withania somnifera collected from different states of India for studying genetic variability. The variability ranges observed at phenotypic
and chemotypic levels were polymorphic. Based on D2 values and PCA (Principal Component Analysis) of phenotypic traits like plant height, no. of branches/plant, no. of seeds/berry,
root length, root diameter and root yield, these 25 accessions were grouped in five clusters. The relative contribution of
each character towards genetic divergence was worked out. Five accessions–AGB002 (Rajasthan), AGB003 (J&K), AGB004 (Madhya
Pradesh), AGB006 (J&K) and AGB009 (Punjab) representing clusters 2 and 4 exhibited maximum intra and inter-cluster divergence.
Cluster 5 representing accession AGB053 (Andhra Pradesh) was having mixed traits. Chemically most of the accessions in cluster
3 showed uniformity in presence of three marker withanolides Withaferin A, Withanone and Withanolide A in the leaves. 相似文献
19.
M. Boczkowska Z. Bulińska-Radomska J. Nowosielski 《Genetic Resources and Crop Evolution》2012,59(4):473-478
Runner bean (Phaseolus coccineus L.) is traditionally cultivated in Poland for dry seeds. The national collection of runner bean maintained in the National
Center for Plant Genetic Resources gathers 152 accessions, which are mainly landraces originated in Poland (68%), Ukraine
(17%) and Slovakia (10%). The collection contains valuable genetic resources for bean breeders and research. The aim of this
study was to describe the level and structure of genetic diversity of three landraces and two commercial cultivars of runner
bean from the national collection in order to assess their genetic potential for breeding. Amplified Fragment Length Polymorphism
analysis included five combinations of selective primers. Analysis of seven genetic diversity parameters reveled fair amount
of genetic variation both in landraces and cultivars. High genetic diversity of commercial cultivars relative those of landraces
suggests that the breeding process leading to their release was rather moderate and most likely included domestic gene pool
of runner bean. Low gene diversity and low Nei’s genetic distance values as well as intergradations among accessions in the
PCoA may indicate reduced variability P. coccineus grown in Poland as a result of its migration pathways. 相似文献
20.
E. E. Radchenko T. L. Kuznetsova M. A. Chumakov I. G. Loskutov 《Genetic Resources and Crop Evolution》2018,65(2):571-576
Among 371 oat accessions from the world collection of the Vavilov Institute of Plant Genetic Resources (VIR) mostly represented by the landraces from Asia (Maritime Territory of Russian Federation, Mongolia, China, Japan, North Korea, India, Iran, Afghanistan, and Bhutan) ninety five were heterogeneous for resistance to the North Caucasus populations of the greenbug (Schizaphis graminum Rondani). Damage ratings of resistance in 47 accessions were high, low level of resistance was identified in 48 genotypes. Higher frequency of greenbug resistant accessions (60.5%) was found among landraces from Mongolia. Lines with high expression of the resistance were selected from seven landraces with wide range of damage ratings. North Caucasus populations of S. graminum from Krasnodar area and Dagestan significantly differ by the frequencies of virulence to host plant. Seven virulence phenotypes differentially interacting with oat genotypes were found in the Krasnodar population and 10 phenotypes were revealed in the Dagestan population. Results of the experiments with the greenbug test clones revealed that all the lines selected from the accessions VIR-2490, VIR-2539, VIR-4074, VIR-12213, VIR-12214 (Mongolia), VIR-6688 (India) and VIR-13624 (North Korea) are protected by diverse alleles of resistance genes which differ from the earlier identified gene Grb3. These lines are supposed to have aphid resistance genes nonidentical to the Grb1 and Grb2. The accessions VIR-6688, VIR-4074 and VIR-12214 possess high resistance to Krasnodar and Dagestan greenbug populations and the accession VIR-13624 is protected by the most effective gene (genes) of resistance to the both populations from North Caucasus. 相似文献