Agronomic Factors in Evaluating Forage Crops II. Predicting Fiber Components (NDF, ADF, ADL) from Crop Leafiness

The relationship of the stage-to-stage changes in alfalfa herbage leaf percentage and the corresponding changes in fiber components represented by neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) was tested in alfalfa crop diverse in stand and canopy ages, stages of maturity harvested across seasons in two separate years. Regression analyses showed that for all fiber components, leaf percentage consistently provided highly significant linear relationships for predicting herbage fiber content; NDF (r²= 0.915), ADF (r²= 0.937) and, ADL (r²= 0.851). These were highly comparable with the application of the mean stage by weight (MSW) system for predicting NDF (r²= 0.946), ADF (r²= 0.891) and ADL (r²= 0.841) thus indicating the practical use of leaf percentage as a good alternative approach for predicting fiber content of the alfalfa crop.     

The Nutritive Value and Yield of Alfalfa Cultivated on Sulphur Postmining Lands

The study was carried out on the reclaimed lands of a bore-hole sulphur mine. The cropping of two succeeding cuts of alfalfa (Medicago sativa L.) was determined and its chemical composition was analyzed. Alfalfa was cultivated on waste lime (flotation tailings), enriched with a humus layer. The levels of basic nutrients, total S, total F, Ca, P, Mg, K, Na, Fe and Mn were determined in alfalfa. The biomass of an average crop was 6830 kg  ha^–1 in the first cut and 14300 kg  ha^–1 in the second one. The yields of dry matter as well as of crude and true proteins were positively correlated with the phosphorus level in the dry matter of alfalfa (P < 0.01). The level of total sulphur was positively linearly correlated with the phosphorus content (P < 0.01). No harmful accumulation of sulphur or fluoride was observed. A deficiency of phosphorus in alfalfa and an abnormal Ca:P weight ratio was found. The S total level was positively correlated with N total concentration. (P < 0.01). The percentage of true protein in crude protein was negatively correlated with total sulphur (P < 0.01). The level of nonprotein nitrogen compounds was positively correlated with S total level (P < 0.01).     

Micrometeorological Influences at High Crop Density on Different Genotypes of Silage Maize

In the year 1979, 1981 and 1982 two-factorial experiments with the factors genotypes and crop densities were conducted, connected with the following agrometeorological analyses:
a) outside the canopies: determination of air temperature, air humidity, wind velocity, precipitation, global radiation and photosynthetically active radiation (PAR)
b) in the interior of the canopies: measurements of air temperature at cobheight, of precipitation components, of the penetration of PAR and of the leaf area index.
The following results were obtained:
a) Influence of high crop densities
1. In high crop densities the greatest part of radiation was absorbed, so that at 30 plants/m² 57% and at 70 plants/m² only 28% of the PAR reached cob height.
2. As a consequence of temperature and decrease of radiation the contents in the leaves of biological energy, of water soluble K.H. and rough protein were reduced.
b) Influence of the leaf posture
3. For genotypes with erected leaf posture the leaf temperatures increased by 0.3°C, on the ground even by 1.2°C above the values of the stand with horizontal leaf posture.
4. Higher air temperatures were measured in the stands with erected leaf posture than in those with a horizontal one.
5. In the cool year 1981 on days with temperatures above 27°C outside the stands, those temperatures were exceeded during 7 hours in canopies with erected leaf posture at a higher plant density (30 plants/m²) and only during 2 hours in canopies with a horizontal leaf posture.     

Some Agronomic and Physiological Aspects of Salt Tolerance in Cotton (Gossypium hirsutum L.)

Evaluation of commonly grown cotton (Gossypium hir-sutum L.) genotypes under saline environment may help to cope with the venture of the crop failure in salt-affected soils. In a pot experiment, four cotton genotypes (MNH-93, NIAB-78. S-12, and B-557) were grown to compare their relative performance on a sandy clay loam soil (original ECe = 1.9 dS m⁻¹) salinized with a salt mixture (Na₂SO₄, NaCl, CaCl₂, MgSO₄ in the ratio of 9:5:5:1 on equivalent basis) to EQ levels of 10 and 20 dS m⁻¹. The crop was raised to the flower initiation stage. The imposed salinity stress exhibited deleterious effect on the germination and vegetative growth with significant differences among the genotypes. Leaf area, stem thickness, shoot (stem + leaves) and root weights decreased with the increase in substrate salinity. NIAB-78 showed the least decline followed by MNH-93. Leaf thickness showed an opposite trend as an increase in this parameter was observed with the rising salinity, the maximum increase being in the case of NIAB-78. Analysis of the leaf sap showed increased Na⁺ and Cl⁻ concentrations and decreased K⁺ concentration with the increase in substrate salinity. A better osmotic adjustment, a lower Na⁺/K⁺ ratio and a lower Cl concentration were found in the leaves of NIAB-78 followed by MNH-93. This contributed towards their better growth performance under saline conditions.     

Effect of Foliar Applications of Glycinebetaine on Stress Tolerance, Growth, and Yield of Spring Cereals and Summer Turnip Rape in Finland

Crop losses caused by environmental stresses might be reduced by applying osmoprotectans to crop canopies. Glycinebetaine is endogenously accumulated by some halophytes under stress conditions and represents such a compound. Glycinebetaine was applied exogenously to barley ( Hordeum vulgare L.), oat ( Avena sativa L.), spring wheat ( Triticum aestwum L.), and summer turnip rape ( Brassica rapa ssp. oleifera DC.) canopies and its optimal concentration was monitored in the greenhouse. In field experiments the response of crop plants to betaine applications was assessed by measuring accumulation of above ground biomass, leaf area index (LAI), leaf chlorophyll, and yield. The optimum betaine concentration producing advantageous effects on growth and crop physiology in turnip rape was close to 0.1 M and for wheat 0.3 M. Such concentrations promoted accumulation of betaine similar to that of halophytes under stress conditions [ca. 200 μmol (g DM)⁻¹]. In the 1993 field experiment peak LAIs were recorded in irrigated wheat and barley treated with 17.5 kg ha⁻¹ betaine applied at 300 1 ha⁻¹. Green leaf area was slightly more persistent in wheat treated twice with 1 kg ha⁻¹ betaine applied at 200 1 ha⁻¹ in 1994, although it was not associated with increased grain yield. Our results indicated that betaine has no actual potential in Finland for the principal grain crops but further studies are needed in stress prone environments to assess the potential of betaine treatments for preventing crop failures.     

Pearl Millet Phenology and Growth in Relation to Thermal Time under Arid Environment

Pearl millet ( Pennisetum glaucum L.R.Br.) hybrid MH 179 was grown under two moisture regimes viz., optimal moisture and rainfed conditions. The field experiment was continued for three consecutive rainy seasons to quantify the pearl millet development with thermal time. The possible influence of variation in natural sowing date on the relationships between crop development and thermal time have been described. At cardinal temperatures of 10°C (base temperature below which pearl millet development ceases), 33°C (optimal temperature for development) and 45°C (maximum temperature at and above which no development takes place), the crop required 1490–1794°Cd thermal time to reach physiological maturity. The thermal time requirement for different developmental stages was influenced by the sowing time and moisture availability during the growing season of the crop.
The leaf tip appearance on the main shoot of pearl millet in relation to thermal time was almost linear under both moisture conditions requiring about 44–50°Cd ± 2.6 °Cd leaf⁻¹, till the appearance of the flag (last) leaf. However, leaf tip appearance on primary tillers was slightly slower and required 53-58°Cd ± 4.7°Cd for each new leaf. Appearance of first primary tiller was later (at 320°Cd after emergence) under the rainfed condition as compared to the crop under the optimal moisture (at 250°Cd).Thereafter, the tiller appearance in relation to thermal time under both moisture conditions was at a linear rate of about 53-56°Cd ± 9.5°Cd tiller⁻¹.
Effect of microclimatic variations, canopy temperature, radiation and photoperiod on the phenology-thermal time relationships have been discussed under both the moisture conditions.     

The Effects of Plant Population Density on the Growth and Chemical Composition of Lablab purpureus Grown for Fodder Production in a Semi-Arid Region

Lablab purpureus was grown in two 6-month field experiments in the Savannah region of Africa, with high rainfall and hot temperatures in the first 2 months, followed by low rainfall and cool temperatures until harvesting in January. The experiments evaluated the effects of plant population density (PPD), using rows at 70-, 110- and 150-cm intervals, on the yield and nutritive value of the crop, and the effect of an interim harvest on response to PPD. Lablab yield ha⁻¹ was increased to a greater extent by an increase in PPD from 110 to 70 cm interrow spacing than by an increase from 150 to 110 cm, and yield plant⁻¹ decreased as PPD increased to a greater extent for double-harvested than for single-harvested plants. This suggested that high PPD in the early stages of growth is important to promote high crop yields in this environment, probably because it mitigates drought effects in autumn. The effects of PPD on herbage composition were small and suggested that plant maturity had not been greatly affected by density. It is concluded that the maintenance of plant cover in the early stages of growth is important for high yields in the Savannah region, which can be achieved by an interrow spacing of no more than 70 cm and avoidance of an interim harvest.     

Nitrogen Fixation, Growth and Yield of Intercropped Mungbean (yigna radiata L.) and Groundnut (Aracbis bypogaea L.) as Affected by the Genotype

¹⁵N-aided investigations were conducted to ascertain the Nj fixation and the nitrogen (N) contribution by mungbean ( Vigna radiata L.) and groundnut ( Aracbis hypogaea L.) when intercropped with maize ( Zea mays ). The study involved growing seven genotypes of the above legumes with maize in alternate rows in two separate experiments. A sole crop of maize was used as the reference crop to determine N2 fixation by the ¹⁵N methodology. Significant genotypic differences in pod yield and stover N content were observed in intercropped mungbean and groundnut. The percentage N derived from the atmosphere showed a genotypic variation of 31 to 45 % (7 to 10 kg N₂ fixed ha⁻¹O in mungbean and 47 to 69 % (9 to 18 kg N₂ fixed ha⁻¹) in groundnut. Harvest index for N varied from 58 to 77 % in mungbean and 55 to 75 % in groundnut. In groundnut, the uptake of soil N was significantly affected by the genotype. Assuming that the N contribution to the soil by the helow-ground plant parts was negligible, the removal of seeds at maturity resulted in a negative N balance in the soil in all the genotypes of mungbean. In groundnut, some genotypes produced a positive N balance in the soil. Owing to high N₂ fixation capacity and low harvest index for N, groundnut showed a greater N supplementing ability than mungbean.     

Growth dynamics of winter wheat in the field with daily fertilization and irrigation

In a field experiment with fertilized and irrigated winter wheat the above-ground crop was sampled once a week. Phenological development, plant density and canopy height were recorded and the green surface areas of leaves, stems and ears were measured. Soil mineral nitrogen was sampled and the field climate monitored. There were four treatments. The daily irrigated/fertilized (IF) and daily irrigated (I) treatments were both irrigated by a drip-tube system. Liquid fertilizer was applied to IF following a logistic function according to calculated plant uptake. A total of 200 kg N ha⁻¹ was applied. Treatment I, control (C) and drought (D) were all fertilized once in spring with 200 kg N ha⁻¹. In treatment D transparent screens were used to divert rainwater. Dry matter production ranged between 1400 in D and 2352 g m⁻² in IF. The corresponding amount of nitrogen uptake ranged between 15.8 and 24.6 g m⁻². After harvest, soil mineral nitrogen was lowest in IF.
An increase in the availability of nitrogen and water enhanced total biomass production as well as grain yield and leaf area. The daily supply of nitrogen according to crop demand delayed nitrogen uptake and increased total uptake. The results suggest that when the nitrogen is supplied in accordance with crop demand, the efficiency with which the applied fertilizer is utilized increases and the risk for nitrogen leaching decreases.     

Light Interception, Leaf Area Index and Dry Matter Yield in Fodder Sorghum at Different Plant Populations

Fodder sorghum (M. P. Chari) was grown at varying populations to examine its influence on light interception, leaf area index and biomass production. DMY, CGR and IPAR depending on LAI reached maximum at LAI 5 of 150 000 plants ha⁻¹ stand and thus yielded higher biomass. Therefore, physiological potential of fodder sorghum crop was found to be maximum at a population density of 150 000 plants ha⁻¹. Further increases in population developed mutual shading and adversely affected the crop growth rate and dry matter accumulation. Significant linear relationships of dry matter yield with IPAR, plant population densities, LAI and CGR clearly indicate the interdependence of these characters.     

Intra-specific Variation for Salt Tolerance in Linseed (Linum usitatissimum L.)

Thirty-six accessions of linseed ( Linum usitatissimum L.) were screened for salt tolerance at the seedling stage after 2 weeks growth in solution culture salinized with 150mol m⁻³ NaCL Considerable variation in salt tolerance was observed in this set of germplasm. Two salt-tolerant accessions, T-5(69-S9)B and LS-2 and two salt-sensitive accessions P-70 and Aver-Rer-Seic, selected at the seedling stage, were grown to maturity in sand culture salinized with 0 (control), 70, 140, and 210 mol m⁻³ NaCl. Both the salt-tolerant accessions exceeded the two salt-sensitive accessions in biomass production, seed yield, and yield components, but the accessions did not differ significantly in seed oil content in the salt treatments. The tolerant accessions accumulated greater amounts of Na⁺ in the shoots and less in the roots compared with the salt-sensitive accessions. The shoot K⁺, K: Na ratios, and K⁺ versus Na⁺ selectivity were lower in the salt-tolerant accessions compared with the salt-sensitive accessions; the reverse was true for their roots. Contributions of Na⁺ and Ca²⁺ to osmotic adjustment were much higher in the two salt-tolerant accessions than in the salt-sensitive ones.
It has been established that there is a great deal of variation for salt tolerance in linseed. The degree of salt tolerance of this crop does not vary during ontogeny. The physiological basis of salt tolerance of this species appears to be associated with the inclusion of Na⁺ in the shoots.     

Conservation of Spring Cut Italian Ryegrass as Round Bale Silage: Effect of Stage of Maturity on Ensiling Characteristics and Forage Nutritive Value

Spring cut Italian ryegrass was harvested at two different stages of maturity: lake vegetative (EARLY) and flowering (LATE). The wilted forage was round baled and then ensiled by wrapping in plastic film. EARLY harvest reduced dry matter (DM) yield significantly as compared to LATE (5.6 vs. 10.5 t ha⁻¹; P < 0.01). However, forage quality both before and after ensiling was better for EARLY with higher crude protein (CP) and non-structural carbohydrates content and a lower amount of cell walls (NDF). Higher pH and the fermentation characteristics confirmed the poor quality of LATE silage, having higher content of butyrate, propionate and acid detergent insoluble nitrogen and a lower concentration of lactic acid.
Dry matter intake of EARLY silage by sheep (average 65.0 ± 7.3 kg LW) fed solely forage ad libitum was 37 % higher than LATE (1386 vs. 1011 g DM day⁻¹; P < 0.05) due to its better palatability and digestibility. In vivo DM digestibility of EARLY silage was 76.1 % while it decreased to 58.2 % for LATE silage. Estimated metabolizable energy content of EARLY silage (11.5 MJ kg⁻¹ DM) was 29 % higher than LATE (8.9 MJ kg⁻¹ DM) resulting as similar to the reference value of a high quality perennial ryegrass silage. The forage conservation system of ensiled round bales is effective and recommended when spring cut Italian ryegrass is harvested at an early stage of maturity.     

Seed Composition, Seedling Emergence and Early Seedling Vigour of Red Kidney Bean Seed Produced at Elevated Temperature and Carbon Dioxide

Understanding the influence of growth temperature and carbon dioxide (CO₂) on seed quality in terms of seed composition, subsequent seedling emergence and early seedling vigour is important under present and future climates. The objective of this study was to determine the combined effects of elevated temperature and CO₂ during seed-filling of parent plants on seed composition, subsequent seedling emergence and seedling vigour of red kidney bean ( Phaseolus vulgaris ). Plants of cultivar 'Montcalm', were grown at daytime maximum/nighttime minimum sinusoidal temperature regimes of 28/18 and 34/24 °C at ambient CO₂ (350 μmol mol⁻¹) and at elevated CO₂ (700 μmol mol⁻¹) from emergence to maturity. Seed size and seed composition at maturity and subsequent per cent emergence, early seedling vigour (rate of development) and seedling dry matter production were measured. Elevated CO₂ did not influence seed composition, emergence, or seedling vigour of seeds produced either at 28/18 or 34/24 °C. Seed produced at 34/24 °C had smaller seed size, decreased glucose concentration, but significantly increased concentrations of sucrose and raffinose compared to 28/18 °C. Elevated growth temperatures during seed production decreased the subsequent per cent emergence and seedling vigour of the seeds and seedling dry matter production of seed produced either at ambient or elevated CO₂.     

Effects of Fungicide on Grain Yield of Barley Grown in Different Cropping Systems

The effect of fungicides and their combination on yield of barley under different nitrogen, slurry and tillage treatments was investigated at Hohenschulen Experimental Station near Kiel, Germany in 1991–97. Various fungicide treatments (no fungicide, and treatment with stem, leaf and ear fungicides and combinations of these), two nitrogen levels (120 and 240 kg N ha⁻¹), two tillage systems (minimum and conventional tillage) and four slurry applications (no application, and autumn, spring and autumn plus spring applications) were used. On average, fungicide application increased barley yield by 1.1 t ha⁻¹. The fungicide treatments could be classified into four types: (1) fungicides against stem diseases, which slightly increased yield by 0.25 t ha⁻¹, very similar to the results for the untreated control; (2) leaf fungicides and ear fungicides applied separately, and fungicides against a combination of stem and leaf diseases, which increased yield by 1.0 t ha⁻¹ on average; (3) fungicides against a combination of ear and stem diseases, which increased the yield by 1.22 t ha⁻¹, and (4) fungicides against a combination of leaf and ear diseases and a combination of stem, leaf and ear diseases, which increased yield by 1.59 t ha⁻¹ on average. The effects of fungicide on the yield were modified by crop husbandry. It can be concluded that application of fungicides against a combination of leaf and ear diseases could increase barley yield and reduce yield variation.     

Effect of Sowing Season and Seeding Rate on the Morphological Traits and Yields in Pea Cultivars of Differing Leaf Types

Determination of optimal N-fertilization rates, N_OR , for crop production that minimize risk of environmental degradation require accurate application of a response model. Several models are available to describe crop yield response to N fertilization. The objective of this work was to compare the relative accuracy of a quadratic, f(N _Q), a modified Mitscherlich, and tanh( N ) models on 48 data sets. Data were collected from a Tara silt loam (fine-silty, mixed, frigid Pachic Udic Haploboroll) over a 6-year period using two maize ( Zea mays L.) hybrids and four tillage treatments, mouldboard plough, chisel plough, ridge tillage and no-tillage. In about one-third of the cases, all models performed about equally well. Generally, the tanh( N ) and modified Mitscherlich models gave better fit between N rate and grain yield data. The N_OR, ranged from about 140 to 170 kg ha⁻¹ for the modified Mitscherlich model, 136 to 184 kg ha⁻¹ for the tanh( N) function, and 124 to 173 kg ha⁻¹ using the f(N _Q) model. Estimated grain yields at these rates ranged between 6.58 ± 1.30 to 7.59 ± 1.69 Mg ha⁻¹ for the 90-day Minnesota maturity rated (MR) hybrid and between 7.52 ± 2.40 to 8.72 ± 1.70 Mg ha⁻¹ for the 95-day MR hybrid.     

Influence of Solar Radiation and Vapour Pressure Deficit on Transpiration Efficiency of Rainfed Sorghum

Besides assimilation, plant water relations are important aspects of physiological basis of productivity of crops in water limited environment. The relationships of photosynthesis rate, transpiration rate, leaf water potential and stomatal conductance with photosynthetically active radiation (PAR) and vapour pressure deficit (VPD) during pre-flowering (panicle initiation to ear emergence) and grain filling (from anthesis to maturity) stages of a sorghum hybrid (cv. CSH-6 ) grown under rainfed conditions were studied. Photosynthesis rate declined when PAR was above 1300 μmol m⁻² s⁻¹. during both the growth stages. Higher transpiration rate during grain filling stage at higher PAR caused the transpiration efficiency to be lower than during pre-flowering stage when PAR was above 1200 μmol m⁻²s⁻¹.Leaf water potential and stomatal conductance decreased with increase in PAR. Leaf water potential was higher during pre-flowering than during grain filling stage but maximum photosynthesis rate was similar during both the growth stages. Changes in VPD did not qualitatively alter the relationships of the physiological variables with PAR.
Decreasing photosynthesis rate and LWP at high PAR suggest that photosynthesis rate was limited by low leaf water potential when PAR was optimal, and by low PAR even when leaf water potential was high in rainfed sorghum during rainy season.     

Effects of Irrigation at Different Growth Stages on Vegetative Growth of Mung Bean, Vigna Radiata (L.) Wilczek, in Dry and Intermediate Zones of Sri Lanka

Mung bean crops in the subhumid zones of Sri Lanka experience significant drought periods. The objective of this study was to quantify the growth response of mung bean to irrigation at different phenological stages and thereby determine the optimum irrigation regime to maximize growth. Four field experiments were conducted at two sites in 1995 and 1996. The crop duration of mung bean was divided into three stages: vegetative (from germination to appearance of first flower), flowering (from appearance of first flower to 75 % pod initiation) and pod-filling (from 75 % pod initiation to maturity). Eight treatments were devised to represent all possible combinations of irrigation at the three stages. Maximum leaf area index (ranging from 0.6 to 2.6 across treatments) and total leaf area duration were increased significantly by irrigation during the vegetative stage. Specific leaf weight decreased and maximum total crop biomass (150–400 g m⁻²) increased with the number of stages irrigated. Irrigation decreased the absolute root biomass and increased the shoot:root ratio. It is concluded that, in this agroclimatic zone of Sri Lanka, irrigation of mung bean during the vegetative stage is critical for maximizing leaf area. However, biomass production can be maximized by increasing the number of stages irrigated irrespective of irrigation at any specific stage.     

Sorghum Leaf Area as a Function of Sixth Leaf Area

This study was undertaken to determine the relationship between sixth leaf area and total leaf area of sorghum ( Sorghum bicolor Moench) to obtain the total leaf area estimate without having to make the time consuming area measurement directly. Ten sorghum varieties were raised at Tamil Nadu Agricultural University campus Coimbatore from which the leaf samples were taken. The allometric relationship derived by logarithmic transformation between total leaf area (TLA) and sixth leaf area measured by leaf area meter (SLA_LW) and TLA and sixth leaf area measured by maximum length and width (SLA_LW) were not different significantly. The allometric relationship for all the ten varieties of pooled data were
TLA = 27.416 (SLA_M)^0.794
and TLA = 14.355 (SLA_LW)^0.857)
Where TLA, SLA_M and SLA_LW are measured in cm². The standard error of estimate equalled to 0.00299 and 0.0035 for the regression of TLA-SLA_M and TLA-SLA_LW respectively. The correlation co-efficients for TLA-SLA_M and TLA-SLA_LW were 0.94 and 0.93 respectively. Among the relationships tried for third, fourth, fifth, sixth and seventh leaf area by meter and length X width verses total leaf area, the SLA verses TLA found to be more suitable.     

CO2 and ozone effects on canopy development of potato crops across Europe

This paper describes the effects of elevated CO₂ (550 and 680 μl l⁻¹) and O₃ (60 nl l⁻¹ O₃ as an 8 h mean), alone or in combination, on canopy development and senescence in potato (Solanum tuberosum L. cv Bintje) across a range of European agro-climatic conditions. The assessments were made within the European CHIP project (CHanging climate and potential Impacts on Potato yield and quality) that was conducted for two growing seasons (1998 and 1999) in free air CO₂ enrichment systems (FACE) and open-top chamber facilities (OTCs) at seven European sites. A comparison of chambered and unchambered experimental plots was included to examine the effects of chamber enclosure. Phenological growth stages, plant height, leaf area index (LAI) and the number of green and yellow leaves were recorded non-destructively throughout the growing season and by a destructive intermediate harvest at maximum leaf area (MLA). In the dynamic growth analysis CO₂ and O₃ effects were studied over three developmental stages: canopy expansion, full canopy and canopy senescence. Chamber enclosures promoted potato crop development (taller plants, more leaves) during the initial growth stages and led to a faster decline of LAI and a higher number of yellow leaves. The growth in ambient plots varied between sites and seasons, as did the scale of the treatment responses. Despite the large background variation, some overall treatment effects could be detected across all sites. Both levels of increased CO₂ reduced final plant height in comparison to ambient concentrations, which indicates a premature ending of the active plant growth. At the stage of full canopy and crop senescence the average number of green leaves was significantly (P<0.05) decreased by 680 μl l⁻¹ CO₂ (OTC experiments) and LAI showed the same tendency (P=0.07). As there was however no indication of a decreased leaf formation during initial growth and at full canopy, this must have been due to an earlier leaf fall. In the FACE experiments LAI had already began to decline at the stage of full canopy at 550 μl l⁻¹ CO₂ but not in ambient CO₂ (DAE×CO₂, P<0.05). These observations strongly indicated that elevated CO₂ induced a premature senescence during full canopy. O₃ did not have an overall detrimental effect on crop development during initial growth nor at full canopy, but did induce a faster reduction of LAI during crop senescence (DAE×O₃, P<0.05). Final plant height was not affected by O₃. There were few CO₂×O₃ interactions detected. There was a suggestion (P=0.06) that O₃ counteracted the CO₂-induced decrease of green leaves at full canopy, but on the other hand during crop senescence the decline of LAI due to elevated O₃ was faster at ambient compared to elevated CO₂ (P<0.05). These responses of canopy development to elevated CO₂ and O₃ help to explain the treatment responses of potato yield within the CHIP project at sites across Europe.     

The Effect of Nitrogen and Phosphorus Fertilization on the Nitrogen Absorption by Sunflowers

Little is known about the effect of fertilization on the N uptake of sunflowers. A 4² factorial trial with 0, 60, 120 and 180 kg N ha⁻¹ and 0, 15, 30 and 45 kg P ha⁻¹ was conducted over three years. The N content and concentration of leaves, stems and capitula were determined at three growth stages. High N levels increased the N content and concentration of all plant parts at all growth stages sharply. High P levels increased the N content of all plant components through better growth. P has an inconsistent effect on N concentration but tended to decrease it. After flowering the crop assimilated 20 to 25 % of the total N. This implies that N applied can still be applied and utilized by the crop at a late stage. This should be substantiated by further research.