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1.
以国家土壤质量安定观测试验站黄绵土区的农田长期定位试验为研究对象,研究长期施肥与覆膜对半干旱区马铃薯产量及其水分利用效率和有机碳的影响。结果表明,在欠水年和正常平水年份,地膜覆盖可显著提高马铃薯产量及其水分利用效率,而丰水年份效果不明显。施肥显著影响马铃薯产量及其水分利用效率,且以有机肥与化肥配施(NPKM)效果最好;在定位施肥初期(前2年),单施化肥(NPK)的效果优于单施有机肥(OM),在第3年两者无差异,而第4年之后则相反。长期施用有机肥(NPKM和OM)较NPK处理0~20 cm土壤有机碳含量显著提高了77.35%和69.65%,有机碳储量分别显著增加了70.68%~76.55%和62.09%~63.31%。不同施肥处理对土壤微生物量碳的影响表现为NPKM>OM>NPK>CK,且差异显著,NPKM和OM较NPK处理土壤微生物量碳含量分别显著增加了43.99%和31.82%,单施有机肥较单施化肥效果显著。长期覆膜与NPK处理可显著提高土壤微生物量碳含量,而对有机碳含量及其储量影响不明显。综上所述,长期有机肥与化肥配施(NPKM)或施用有机肥(OM)可显著提高旱地... 相似文献
2.
以国家土壤质量安定观测实验站黄绵土区长期定位试验为研究平台,研究长期施肥对半干旱区小麦田土壤团聚体分布及其有机碳含量的影响。结果表明:0~20 cm土层土壤团聚体含量随粒级减小总体呈先降低后增加的变化趋势,团聚体有机碳含量随粒级减小呈增加的变化趋势。长期化肥与有机肥配施(NPKM)和单施有机肥(OM)较单施化肥(NPK)处理显著增加了>0.25 mm团聚体含量和团聚体平均质量直径,提高了>1 mm粒级土壤团聚体对有机碳的贡献率。各粒级土壤团聚体有机碳含量及团聚体有机碳储量均随着土层的加深而降低,>1 mm粒级团聚体有机碳对长期施用有机肥的农田有机碳变化响应敏感。NPKM和OM较NPK处理显著增加了0~20 cm土层土壤团聚体有机碳含量及其储量,其中0~10 cm土层团聚体有机碳储量分别增加了34.1% 和25.2%,10~20 cm土层分别增加了26.89% 和17.95%,而长期NPK处理对团聚体有机碳含量及其储量影响不明显。综上所述,在西北黄土丘陵半干旱区黄绵土条件下,长期施用有机肥(NPKM和OM处理)可显著提高耕层>0.25 mm团聚体含量及其稳定性,增加团聚体有机碳储量,土壤增碳培肥效果显著,而单施化肥对改善农田土壤团聚体结构及其固碳培肥效果不明显,需加强与有机肥配施从而实现土壤培肥。 相似文献
3.
以国家土壤质量安定观测实验站黄绵土区的农田长期定位试验为研究平台,研究长期施肥与覆膜对半干旱区马铃薯农田土壤团聚体分布及其有机碳含量的影响。结果表明:耕层土壤团聚体占比随着粒径的减小呈先降低后增加的变化趋势,团聚体有机碳含量随粒径减小呈增加趋势。施肥显著影响马铃薯农田土壤团聚体分布及有机碳含量。长期化肥与有机肥配施(NPK+OM)和单施有机肥(OM)较单施化肥(NPK)显著增加>0.25 mm团聚体含量(R0.25)和平均质量直径(MWD),显著提高大团聚体(>1 mm)对有机碳的贡献率。长期施用有机肥(NPK+OM和OM)较单施化肥(NPK)显著增加0~20 cm土层土壤团聚体有机碳储量,增幅达63.5%~82.8%。长期覆膜导致>2 mm粒径的大团聚体数量和MWD显著下降,覆膜与单施化肥(NPK)对团聚体有机碳含量及其储量影响不明显。综上所述,在西北黄土丘陵半干旱区和黄绵土质地条件下,长期化肥与有机肥配施(NPK+OM)或单用有机肥(OM)可显著提高耕层土壤大团聚体含量(R0.25)及其稳定性,促进新碳向大团聚体富集,增加团聚体有机碳储量,有利于农田土壤固碳。地膜... 相似文献
4.
研究长期不同施肥对双季玉米种植制度下旱地红壤理化性质和玉米产量季节性变化的影响,明确产量变化驱动因素,可为该区耕地质量培育和合理施肥提供理论支持。以1986年设置的旱地红壤长期定位施肥试验为平台,选取不施肥(CK)、单施化肥(NPK)、单施新鲜猪粪(OM)、化肥和新鲜猪粪配施(NPKM)4个处理,探索不同施肥制度对双季玉米成熟期0~20 cm和20~40 cm土层土壤理化性质以及玉米产量的影响。结果表明,与CK处理相比,NPKM处理在春玉米和秋玉米成熟期0~40 cm土层土壤容重显著降低,大团聚体(>250μm)含量、团聚体平均重量直径(MWD)、土壤pH、有机碳(SOC)、速效氮(AN)、有效磷(AP)及速效钾(AK)等养分含量均显著提高,春玉米和秋玉米产量分别提高了6.1倍和15.1倍。与CK处理相比,NPK处理0~20 cm土层土壤容重在春玉米和秋玉米成熟期分别提高了1.13%和2.49%,pH则分别降低0.22和0.48个单位。与NPK处理相比,OM和NPKM处理0~20 cm土层土壤容重分别降低2.5%和3.2%,MWD分别增加了20.9%和43.6%,pH分别提高了1... 相似文献
5.
长期施肥对潮土不同层次活性有机质及碳库管理指数的影响 总被引:3,自引:1,他引:2
为探索长期不同施肥下潮土不同土层活性有机质和碳库管理指数变化特征,选取不施肥对照(CK)、单施氮磷钾肥(NPK)、NPK配施有机肥(NPKM)和NPK配施秸秆还田(NPKS)4个典型施肥处理,测定并分析土壤高、中、低活性有机质含量以及碳库管理指数(CMI)变化特征。结果表明:各处理土壤有机质含量均随土层加深而降低,且处理间差异随土层深度缩小。施肥处理相对不施肥对照(CK)均明显提升了不同土层的有机质含量,以NPKM处理最高,达到13.91~33.55 g/kg。各处理以非活性和低活性有机质为主,且其比例随着土层加深而增加,其中CK处理比例最高,分别为35.6%~56.6%和17.7%~50.7%。施肥处理对土壤CMI均有提高,在0—40 cm土层,NPKS的高活性CMI最高,分别为149.54,147.01,237.65;而在40—60 cm土层,以NPKM处理的高、中活性CMI最高,达到237.65,537.67。综上所述,各处理土壤有机质和活性有机质含量均随土层加深而降低,且处理间差异随土层深度缩小;氮磷钾配施有机肥提升总有机质及活性有机质处理效果最佳。在上层土层(0—40 cm)氮磷钾配施秸秆有助于提升高、中活性有机质的碳库管理指数;而在下层土层(40—60 cm)则以氮磷钾配施有机肥最优。整体来看,NPK配施有机肥(NPKM)对土壤的肥力提升效果最好,NPK配施秸秆还田(NPKS)次之。 相似文献
6.
《农业环境与发展》2021,(5)
为研究长期有机无机肥配施对红壤性水稻田作物产量、土壤微生物生物量及有机碳分子结构的影响,以始于1984年的江西红壤性水稻田长期定位试验为平台,选取的试验处理包括:不施肥(CK)、单施化肥(NPK)和等养分条件下70%化肥配施30%有机肥(NPKM1)、50%化肥配施50%有机肥(NPKM2)、30%化肥配施70%有机肥(NPKM3),采用固体~(13)C核磁共振测定了土壤有机碳组分含量,分析了土壤化学指标和土壤微生物生物量碳(Microbial biomass carbon,MBC)和微生物生物量氮(Microbial biomass nitrogen,MBN)。结果表明,连续34年的不同施肥处理显著影响了水稻产量、土壤微生物生物量及土壤有机碳(SOC)分子结构。与NPK处理相比,有机肥配施(NPKM1、NPKM2、NPKM3)提高了水稻产量,增幅为6.5%~7.7%(P0.05),中低有机肥配施比例(30%和50%)稳产效果更优。长期单施化肥使土壤严重酸化,而配施有机肥可减缓土壤酸化。长期施肥处理MBC和MBN较CK处理分别显著提高17.0%~71.1%和104.1%~267.0%,但MBC/MBN下降,有机无机肥配施处理较NPK处理提高了微生物熵。长期单施化肥主要提高了烷基碳的相对含量,而配施有机肥同时提高烷基碳和烷氧碳(甲氧基/含氮烷基碳)含量,有利于土壤活性有机质累积。Pearson相关性分析表明土壤微生物生物量与SOC、氮磷养分指标及甲氧基/含氮烷基碳呈显著或极显著正相关,与芳基碳和羧基碳呈显著负相关。冗余分析显示SOC、有效磷、速效钾及烷基碳等对水稻产量的影响较大。研究表明,在供试条件下,长期实行中低比例有机肥配施化肥有利于提高土壤养分和土壤微生物生物量,并改善土壤有机质结构,是维持作物高产和提升土壤质量的有效施肥措施。 相似文献
7.
长期不同施肥下黑土与灰漠土有机碳储量的变化 总被引:5,自引:1,他引:5
采用长期试验,研究了20年不同施肥下1 m深黑土与灰漠土有机碳含量与碳储量的剖面变化。结果表明,单施化肥和不施肥对黑土1 m土层有机碳储量没有显著影响,但灰漠土略有降低。有机肥配施化肥能显著提高土壤有机碳含量和储量。高量有机肥配施化肥(NPKM2)能提高020 cm和2040 cm土层土壤有机碳含量,黑土分别提高56.6%和49.6%、灰漠土提高143.1%和46.9%;常量有机无机配施(NPKM)效果较差,增幅分别为黑土35.1%和35.3%,灰漠土80.2%和4.1%。两种土壤1 m土体的有机碳储量,NPKM2处理分别提高了C 30.7 t/hm2与C 40.6 t/hm2。显然,有机无机肥配施可以显著提高1 m深土体中有机碳储量,主要是由于提高了040 cm土层土壤有机碳含量。 相似文献
8.
不同施肥模式对南方黄泥田耕层有机碳固存及生产力的影响 总被引:5,自引:3,他引:2
【目的】探讨南方丘陵黄泥田不同施肥对耕层土壤有机碳固存及生产力的影响,促进区域农田固碳减排和作物高产。【方法】基于32年的长期定位试验,研究不施肥(CK)、单施化肥(NPK)、化肥+牛粪(NPKM)、化肥+全部稻草还田(NPKS)处理下,历年水稻产量、代表性年份耕层土壤有机碳含量及固碳速率的变化。【结果】NPK、NPKM、NPKS处理下水稻历年平均产量分别较CK高67.1%、88.1%和84.2%,差异显著,且NPKM、NPKS处理与NPK处理间亦具有显著差异。NPK、NPKM与NPKS处理耕层土壤有机碳历年平均含量比CK高8.9%~36.8%其中NPKM最高且亦显著高于NPKS与NPK处理。与初始土壤相比各处理有机碳含量增加1.84~5.26g/kg。以每10年为评价周期,NPKM、NPKS处理的固碳速率与CK及NPK差异均显著,其中双季稻年份NPKM与NPKS处理固碳速率分别是CK的2.38倍和1.98倍,是NPK处理的1.59倍与1.32倍但NPK处理与CK间差异不显著。稻田系统年均有机碳输入与有机碳固存间存在极显著幂函数关系,施肥土壤有机碳含量变化与籽粒产量变化间亦呈极显著正相关。【结论】南方黄泥田化肥配施有机肥或配合秸秆还田较单施化肥稳步提升水稻产量。长期不施肥土壤有机碳仍可维持低幅度增长,随着土壤有机碳含量升高,固碳效率逐步降低。化肥配施有机肥或配合秸秆还田较单施化肥明显提高了土壤的固碳速率二者均是提高黄泥田生产力与固碳能力的双赢措施。 相似文献
9.
长期施肥对土黑碳积累的影响 总被引:1,自引:0,他引:1
通过长期定位试验,探讨了20年不施肥(CK)、 单施化肥(NPK)、 秸秆和化肥配施(SNPK)、 常量有机肥和化肥配施(M1NPK)以及高量有机肥和化肥配施(M2NPK)5个施肥处理对土中黑碳含量及积累的影响。结果表明: 与CK处理相比,NKP处理对黑碳含量和积累没有明显影响; 有机肥和化肥配施(M1NPK、 M2NPK)对耕层(020 cm)土壤黑碳含量的影响较大,与CK处理相比,在土壤表层(05 cm),M1NPK、 M2NPK分别提高了黑碳含量的108%和134%, 510 cm土层中黑碳含量增幅最高,分别提高164%和176%,在整个土层,M1NPK、 M2NPK处理分别增加了1.51和1.55倍; 秸秆和化肥配施(SNPK)下表层黑碳含量也有所增加,但增加幅度相对施用有机肥来说明明显较小。施肥对黑碳含量的影响主要发生在土壤表层,深层土壤黑碳受施肥影响较小。 相似文献
10.
长期施肥对小麦-玉米作物系统土壤腐殖质组分碳和氮的影响 总被引:7,自引:1,他引:6
通过对华北平原小麦–玉米轮作农田生态系统18年田间施肥试验,研究了长期不同施肥处理对耕层(0—20 cm)土壤腐殖质及活性腐殖质组分碳和氮的影响。试验设化肥NPK不同组合(NPK、NP、NK、PK),全部施用有机肥(OM),一半有机肥+化肥NPK(1/2OMN)及不施肥(CK)共7个处理。结果表明,各施肥处理均能在不同程度上增加土壤腐殖质(胡敏酸、富里酸和胡敏素)及活性腐殖质(活性胡敏酸和活性富里酸)组分碳和氮含量,提高可浸提腐殖质(胡敏酸和富里酸)及活性腐殖质组分碳和氮分配比例;但施肥对土壤活性腐殖质组分碳和氮含量的增加率均分别高于腐殖质组分碳和氮。各处理土壤腐殖质及活性腐殖质组分碳和氮含量均为OM处理最高,且有机肥与化肥NPK配施高于单施化肥各处理;而化肥处理中NPK均衡施用效果最好。说明施用有机肥、有机肥与化肥NPK配施及化肥NPK均衡施用是增加土壤腐殖质及活性腐殖质组分碳和氮的关键;活性腐殖质组分碳和氮较腐殖质组分碳和氮对施肥措施的响应更灵敏。 相似文献
11.
Joseph J. Pignatello 《Journal of Soils and Sediments》2012,12(8):1241-1256
Purpose
This article reviews our current understanding about how organic chemicals and water interact dynamically with, and therefore coevolve with, soil and sediment natural organic matter (NOM). NOM can be regarded as a polymer-like phase that responds to the input of organic compounds in ways analogous to synthetic polymers.Methods
Sorption selectivity of organic compounds is shown to result in part from the three-dimensional microstructure of NOM related to its glassy character. Sorption to NOM conforms to polymer theory by exhibiting isotherm shape and irreversible behaviors characteristic of the glassy organic physical state. The glassy state is a metastable state characterized by the presence of excess free volume (holes).Results
In polymers and NOM, incoming molecules preferentially occupy holes due to the absence of a cavitation penalty. Incoming molecules can enlarge existing holes and create new holes that do not relax completely when the molecules leave. The physical changes in NOM induced by sorption result in hysteresis in the isotherm that persists indefinitely at ambient temperature.Conclusions
Sorption selectivity and hysteresis have important implications for the fate and bioavailability of contaminants. 相似文献12.
不同类型有机物料的有机磷组成及生物有效性 总被引:2,自引:1,他引:1
[目的]研究不同有机物料的有机磷组成及其作为磷源施用后的供磷能力,为化肥磷的有机替代奠定理论基础.[方法]供试有机物料包括粪肥类(猪粪、羊粪)、绿肥类(豌豆、苜蓿和绿豆)、秸秆类(小麦秸秆、玉米秸秆和油菜秸秆).分析了8种有机物料的全磷、有机磷含量和C/P值,采用Bowman-Cole方法测定了有机磷中的活性(LOP)... 相似文献
13.
Sándor Hoffmann Elke Schulz Gábor Csitári László Bankó 《Archives of Agronomy and Soil Science》2013,59(6):627-635
Abstract The influence of farmyard manure (FYM) and equivalent mineral NPK application on organic matter content, hot water extractable carbon (HWC), microbial biomass C (Cmic), and grain yields in a long-term field experiment was assessed after 40 years in Hungary. The unfertilized plot, FYM fertilized plots and plots fertilized with equivalent NPK fertilizer contained 0.99%, 1.13% and 1.05% total organic carbon (TOC) respectively. Compared to the unfertilized plot, FYM application resulted in 8.2% higher TOC than equivalent NPK fertilization. The highest TOC was only 1.21%, much lower than expected for a soil containing 21.3% of clay. The quantity of HWC varied depending on the type of fertilization: Compared to control, FYM treatments lead to 29% more HWC than mineral fertilization (FYM: 328 mg kg?1; NPK: 264 mg kg?1). The impact of FYM and equivalent NPK fertilizer on Cmic was contrary. FYM and NPK resulted in 304 and 423 mg kg?1 Cmic, respectively. The difference was 119 mg kg?1; 42% as compared to the unfertilized plot. Despite the higher HWC content, FYM treatments lead to significantly less (35%) grain yields than equivalent NPK doses; Cmic content showed closer correlation to grain yields. 相似文献
14.
Purpose
Microbial decomposition of soil organic matter (SOM) is generally believed to be heterogeneous, resulting in the preferential loss of labile compounds such as carbohydrates and proteins and the accumulation of recalcitrant compounds such as lipids and lignin. However, these fractions are difficult to measure directly in soils. We examined patterns in the biomolecular composition of SOM and hot-water-extractable organic matter (HWEOM) by using a molecular mixing model (MMM) to estimate the content of carbohydrates, protein, lipids, and lignin.Materials and methods
Organic-horizon soils from Spodosols at the Hubbard Brook Experimental Forest in NH, USA were analyzed for this study. The MMM uses data from elemental analysis (C, H, and N) and 13C nuclear magnetic resonance spectroscopy with cross-polarization and magic-angle spinning to estimate the percentage of total C in the various classes of biomolecules.Results and discussion
Carbohydrate content decreased from about 50 % of the C in recent litter to approximately 35 % in the bottom of the humus layer. Lipids accounted for about 18 % of C in recent litter and increased to 40 % in the lower humus layers. The HWEOM fraction of SOM was dominated by carbohydrates (40–70 % of C). Carbohydrates and lipids in HWEOM exhibited depth patterns that were the opposite of the SOM. The results from the MMM confirmed the selective decomposition of carbohydrates and the relative accumulation of lipids during humus formation. The depth patterns in HWEOM suggest that the solubility of carbohydrates increases during decomposition, while the solubility of the lipid fraction decreases. The MMM was able to reproduce the spectral properties of SOM and HWEOM very accurately, although there were some discrepancies between the predicted and measured H/C and O/C ratios.Conclusions
The MMM approach is an accurate and cost-effective alternative to wet-chemical methods. Together, carbohydrates and proteins account for up to 85 % of the C in HWEOM, indicating that the HWEOM fraction represents a labile source of C for microbes. Humification resulted in a decrease in carbohydrate content and an increase in lipids in SOM, consistent with investigations carried out in diverse soil environments. 相似文献15.
科学减施化肥用量,合理配施有机肥对土壤肥力提升、农产品增质和减轻面源污染有着重要的意义。我国农业有机废弃物资源丰富,有机养分总量达到约 7 519.5万 t,其中 N、P 2O5、K2O养分总量分别为 3 096.3万、1 174.1万、3 249.1万 t,但是目前资源利用率不足 40%,不仅造成优良有机养分资源的浪费,同时带来环境污染风险的问题。如何高效利用有机肥资源是我国目前亟需解决的问题。本文总结有机肥相关文献和他人研究结果,综述了我国有机肥资源数量、利用现状,针对施用的问题,对产业发展提出了一些建议。 相似文献
16.
平菇栽培废料等有机肥对土壤活性有机质和土壤酶活性的影响 总被引:13,自引:0,他引:13
通过大田试验研究了不施有机肥(CK)、施用平菇栽培废料(T1)、施用干腐熟牛粪(T2)和烘干鸡粪(T3)在种植黄瓜01~50.d内土壤中活性有机质和4种土壤酶活性的变化。结果表明:施入不同有机肥对土壤总有机质含量的影响为烘干鸡粪平菇栽培废料干腐熟牛粪对照;对活性有机质含量的影响为平菇栽培废料烘干鸡粪干腐熟牛粪对照;施用平菇栽培废料的土壤中脲酶、转化酶和脱氢酶活性最高,施用干腐熟牛粪的土壤中过氧化氢酶活性最高。相关性分析显示,脲酶、转化酶和脱氢酶活性与土壤活性有机质显著相关。用平菇栽培废料做有机肥能有效提高土壤活性有机质含量和土壤酶活性。 相似文献
17.
In water and waste treatment, cationic polyelectrolytes can function as either primary coagulant or coagulant aid because they are positively charged. It has been known that most of the colloidal and suspended impurities in natural water have negative surface charges. Cationic polyelectrolyte will neutralize the impurities or pollutants, then agglomerate them into larger masses for rapid solid-water separation by sedimentation, flotation, centrifugation, filtration, or reverse osmosis. Anionic polyelectrolyte are negatively charged and function as coagulants in water and waste treatment. They must be applied along with positively charged coagulants which neutralizes the negative charge on the colloidal and suspended impurities. In a water softening process, where precipitation particles are positively charged, however, anionic polyelectrolytes alone can coagulate. Nonionic organic polymers, however, can only function as coagulant aids which should be introduced at a point shortly after flocculation first occurs. Nonionic polymers will coat the existing floc particles so that when the flocs collide, they will adhere to one another, thus forming larger and heavier masses. This paper initially describes the various applications of organic polymers in the fields of environmental, chemical and biomedical engineering. Subsequently a new technique for the accurate, quantitative determination of polyelectrolytes in the 0.00002 N to 0.005 N concentration range is introduced. The principles, apparatus, reagents, and analytical procedures of the method are presented in detail. The test procedure developed can also measure the net charges of colloidal substances, this can be used for chemical coagulation process control. 相似文献
18.
长期定位施肥对潮土有机氮组分和有机碳的影响 总被引:20,自引:0,他引:20
利用河南封丘潮土的13年长期施肥试验,采用Bremner法研究了潮土耕层有机氮组成的变化,分析了长期施肥对土壤有机氮组份和有机碳含量的影响。与不施肥和单施化肥相比,施有机肥或有机肥与化肥配施显著提高了土壤全氮、酸解有机氮、酸解铵态氮、氨基酸态氮、非酸解有机氮和有机碳的含量。有机氮主要由酸解铵态氮和氨基酸态氮组成,其次为酸解未知态氮和非酸解有机氮,氨基糖态氮含量最小。施有机肥尤其利于氨基酸态氮和非酸解有机氮的形成。施肥处理的酸解有机氮占全氮的比例减小,主要是由氨基酸态氮、酸解铵态氮占全氮的比例减小所致。与1989年试验开始时的土壤初始值相比,施有机肥能提高土壤全氮含量和有机质含量。在供应等氮磷钾的情况下,有机无机配施增加了土壤供氮能力、有机质含量和作物产量,是维持土壤肥力和保护环境最优的施肥方式。 相似文献
19.
《Soil biology & biochemistry》1986,18(6):643-647
The organic phosphorus components of leaf litter from a secondary forest in Nigeria were characterized as phospholipids, acid soluble esters, LiOH-extractable esters and residual phosphate. During incubation of the litter at 30°C for 8 months all four organic fractions were synthesized and the content of inorganic phosphate decreased.During cultivation of the same field site over a period of 22 months, the IP5 + 6, content of the soil did not change significantly, but there were significant decreases of phospholipids and of high molecular weight components. The high molecular weight components, which were not identified, represented the most important source of organic phosphorus that became available to plants, but phospholipids may also have been a source.The results indicate that the inositol phosphates in leaf litter were in too low concentration to have provided the amounts found in the soil. 相似文献
20.
The effects of organic and mineral fertilization on four soil organic matter (SOM) fractions (non-protected, physically protected, chemically protected, and biochemically protected) and microbial community composition were investigated by sampling soil of a 35-year-long fertilization experiment. The SOM fractions were investigated by combined physical and chemical approaches, while microbial community composition was determined by phospholipid fatty acid analysis (PLFA). Organic C (SOC) was primarily distributed within the microaggregate-protected particulate organic matter (iPOM) and the hydrolysable and non-hydrolysable silt-sized (H-Silt, NH-Silt) fractions, which accounted for 11.6–16.9, 23.4–28.9, and 25.4–30.6% of the total SOC content, respectively. The contributions of these “slow” fractions (iPOM, H-Silt, NH-Silt) to the increased SOC were 178–293, 118–209, and 85–109% higher after long-term sole manure or manure in combination with inorganic N fertilization compared with unfertilized soil (control). The combination of manure and mineral fertilizers increased the coarse and fine non-protected C (cPOM and fPOM) contents much more (34.1–60.7%) than did manure alone. PLFAs, bacteria, G (+) bacteria, and actinomycete abundances were the highest in soil with manure, followed by soil treated with manure combined with mineral N. The addition of inorganic and organic fertilization both altered the microbial community composition compared with the control. All SOM fractions contributed to 81.1% of the variance of the PLFAs-related microbial community composition by direct and indirect effects. The change in coarse unprotected particulate organic matter (cPOM) was the major factor affecting soil microbial community composition (p < 0.001). Our study indicates that physical, chemical, and biochemical protection mechanisms are important in maintaining high SOC level after the addition of manure. A close linkage between soil microbial community composition and cPOM suggests that C availability is an important factor for influencing microbial composition after long-term inorganic and organic fertilization. 相似文献