经皮髓芯减压结合介入外置管治疗早期股骨头缺血性坏死(附50例)

目的：观察经皮髓芯减压结合介入外置管治疗早期股骨头缺血性坏死的临床疗效。方法：经X线平片、CT、MRI检查及临床证实的早期股骨头缺血性坏死50例病人，首先行股骨头经皮髓芯减压术，同时行经皮介入外置管治疗，保留外引流管1周。结果：①骨髓静脉造影复查显示50例回流通畅，回流时间加快。②治疗后1年X线平片复查显示：35例复查显示骨质完全修复，余15例有不同程度的骨质修复。③治疗后1年．MRI复查显示：48例显示骨髓水肿消退，仅2例显示残留散在小斑点状骨髓水肿信号病灶。④治疗后1年临床症状随访结果：43例治疗后疼痛完全消除，大部分缓解5例，部分缓解2例，未改善0例，其临床症状改善率达100％。结论：经皮髓芯减压结合介入外置管治疗早期股骨头缺血性坏死，能明显改善股骨头的血液循环，促进股骨头坏死区的修复和重建。     

Adsorption to fish sperm of vertically transmitted fish viruses

More than 99 percent of a vertically transmitted fish rhabdovirus, infectious hematopoietic necrosis virus, was removed from suspension in less than 1 minute by adsorption to the surface membrane of sperm from two genera of salmonid fishes. The vertically transmitted, infectious pancreatic necrosis virus adsorbed to a lesser degree, but no adsorption occurred with a second fish rhabdovirus that is not vertically transmitted. Such adsorption may be involved in vertical transmission of these viruses.     

Hox10 and Hox11 genes are required to globally pattern the mammalian skeleton

Mice in which all members of the Hox10 or Hox11 paralogous group are disrupted provide evidence that these Hox genes are involved in global patterning of the axial and appendicular skeleton. In the absence of Hox10 function, no lumbar vertebrae are formed. Instead, ribs project from all posterior vertebrae, extending caudally from the last thoracic vertebrae to beyond the sacral region. In the absence of Hox11 function, sacral vertebrae are not formed and instead these vertebrae assume a lumbar identity. The redundancy among these paralogous family members is so great that this global aspect of Hox patterning is not apparent in mice that are mutant for five of the six paralogous alleles.     

Quantitative trait loci for the number of vertebrae on Sus scrofa chromosomes 1 and 7 independently influence the numbers of thoracic and lumbar vertebrae in pigs

Although quantitative trait loci(QTLs) for number of thoracic-lumbar vertebrae have been identified on Sus scrofa chromosomes(SSCs) 1 and 7, the influence of these QTLs on the thoracic and lumbar vertebrae is not clear. The aim of this study was to identify single nucleotide polymorphisms(SNPs) associated with total number of thoracic-lumbar vertebrae and for each trait(number of thoracic and lumbar vertebrae) separately. A total of 581 individuals from an F2 Large White×Minzhu population were genotyped using an SNP60 K chip. Performing a genome-wide association study(GWAS) for total number of thoracic-lumbar vertebrae, 38 significant SNPs were identified in two QTL regions located on SSC1 and SSC7. Performing a GWAS for number of thoracic vertebrae only, 72 significant SNPs were located on SSC7. While performing a GWAS for number of lumbar vertebrae only, 17 significant SNPs were identified on SSC1. Gene mining suggested that the gene encoding orphan nuclear receptor, germ cell nuclear factor(NR6A1) on SSC1 was a strong candidate affecting the number of lumbar vertebrae in pigs. Additionally, genes encoding vertnin(VRTN), prospero homeobox 2(PROX2), Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog(FOS), and transforming growth factor beta 3(TGFB3) may be important candidates affecting the number of thoracic vertebrae in pigs. QTLs on SSC1 and SSC7 independently influenced the numbers of thoracic and lumbar vertebrae. These results shed light on the complex genetic background of vertebrae development in pigs.     

Hyaluronate in vasculogenesis

Limb buds of chicken embryos contain within the peripheral mesoderm an avascular zone that is rich in hyaluronic acid. Epithelial tissues that synthesize large amounts of hyaluronic acid relative to other glycosaminoglycans caused avascularity when implanted into normally vascular wing mesoderm. Epithelia that synthesize little hyaluronic acid did not cause avascularity. Elvax implants containing hyaluronic acid caused the formation of avascular zones, whereas similar implants containing other glycosaminoglycans did not give rise to avascular zones. Hyaluronic acid may thus play a role in determining the location of blood vessels in the embryo.     

绵羊HOXC8基因的DNA甲基化研究

本研究测定了蒙古羊和德国美利奴肉羊,道塞特肉羊,澳洲美利奴细毛羊Hoxc8全基因序列。蒙古羊与这3个外国品种的Hoxc8基因,只有内含子存在8个碱基差异。但是exon-1的甲基化模式存在极大差异。蒙古羊14枚胸椎个体的甲基化程度高于13枚胸椎个体。蒙古羊甲基化程度普遍高于外国品种。蒙古羊特有的可遗传甲基化位点共有6个(5,69,1,62,6,27),国外品种在这些位置不发生甲基化。并且,这6个位点的甲基化程度高低可能决定了第14枚胸椎的长短和骨化程度。     

袖珍椰子根际寄生线虫种类鉴定

于福州市多家园艺场随机采集65份袖珍椰子根部及根际样本,经鉴定,共有7个属8种植物寄生线虫:咖啡短体线虫、饰环矮化线虫、双宫螺旋线虫、双尾滑刃线虫、蘑菇滑刃线虫、燕麦真滑刃线虫、食菌茎线虫和普通丝尾垫刃线虫.利用次氯酸钠—酸性品红染色法证实,咖啡短体线虫可大量存在于袖珍椰子根部及根际介质中,并造成根系坏死、腐烂.     

桃红四物汤对大鼠创伤性股骨头缺血坏死模型外周血中EPCs表达影响

目的 通过检测桃红四物汤对创伤性股骨头缺血坏死模型外周血中血管内皮祖细胞（endothelial progenitor cells,EPCs）表达的影响,探讨桃红四物汤治疗创伤性股骨头缺血坏死的具体作用机制。方法 将60只实验大鼠适应性喂养1周后随机分为两组,正常对照组15只,造模组45只,造模组按照圆韧带离断结合剥离切除术造模,造模4周后,两组各随机抽取5只,对比检测造模是否成功,再将造模组随机分成4组,即桃红四物汤低、中、高剂量组及模型组,每组10只,桃红四物汤低、中、高剂量分别灌服4、8、16 g/kg桃红四物汤汤剂,余下二组灌服等容量的生理盐水,药物干预8周后对股骨头大体形态观察、组织形态学观察、空骨陷窝率检测,并用流式细胞仪检测大鼠外周血中EPCs（CD34+、CD133+）水平。结果 桃红四物汤三组灌胃8周后空骨陷窝率明显低于模型组,高于正常对照组,差异有统计学意义（P<0.05）;桃红四物汤三组灌胃8周后外周血中EPCs（CD34+、CD133+）表达量明显高于模型组,低于正常对照组,差异有统计学意义（P<0.05）。结论 桃红四物汤能明显促进创伤性股骨头坏死大鼠模型外周血中EPCs（CD34+、CD133+）的表达,进一步从微观上及分子水平说明其对创伤性股骨头坏死有防治作用。     

东北红豆杉内生真菌的多样性

自东北红豆杉(Taxus cuspidata Sieb．et Zucc．)根、茎、叶中分离获得78种内生真菌,经形态学和ITS分析,确认分别属于子囊茵亚门的3个属和半知茵亚门的23个属,表明东北红豆杉内生真菌具有丰富的物种多样性,其中有4个菌种能产紫杉醇。     

The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months

Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity. In this study, we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S rRNA gene over the same period in lambs. The results showed that lambs’ growth performance, the serum parameters, fecal bacterial community and fecal bacterial functions were all affected (P<0.05) by age of the lambs. Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply (P<0.05) after weaning at 60 days. The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age, increased (P<0.05) sharply after 30 days of age. Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators, including negative (P<0.05) correlations between the relative abundance of Lachnospiraceae UCG-010, Eubacterium coprostanoligenes group, Ruminococcaceae UCG-005, Ruminococcaceae UCG-009, Ruminococcaceae UCG-013, Ruminiclostridium 6, Ruminococcaceae UCG-008, and Oscillibacter with serum concentrations of lipopolysaccharide (LPS), D-lactate dehydrogenase (DLA), immunoglobulin (IgA, IgM, and IgG), and cytokines (interleukin-1β (IL-1β), IL-6, IL-12, and IL-17), tumor necrosis factor-α (TNF-α), and the relative abundance of these genera increased from 45 days of age. In conclusion, these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs, and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.     

福建野生彩叶植物的地理分布与资源调查

福建省野生彩叶植物资源丰富,对其观赏特性、地理分布和资源现状等进行了调查和分析.结果表明,福建有野生彩叶植物54科106属165种,分别占本区系高等植物科属种总数的21.86％、6.64％、3.50％.其中乔木类32科54属86种,灌木类24科29属39种,小乔木类19科25属29种,藤本5科5属5种,草本4科5属6种...     

山西农业大学校园大型真菌种类研究[Ⅰ]

通过对山西农业大学校园内,林木和草地生长的大型真菌种类的调查,鉴定结果表明:该校园大型真菌隶属担子菌门,其中伞菌目7个种隶属于5个属(Agaricus,Mycena,Schizophyllum,Coprinus,Calvatia);多孔菌目4个种隶属于4个属(Oligoporus,Ganoderma,Coriolus,Phellinus);鬼笔目2个种隶属于2个属(Simblum,Lysusus);鸡油菌目1个种隶属于1个属(Cantharellus);花耳目1个种隶属于1个属(Dacryopinax)。其中伞菌目和多孔菌目中种类较多。     

胡杨根际真菌与内生真菌多样性研究

对胡杨根际真菌与内生真菌进行分离,共获得真菌344株,经显微形态观察鉴定,分别为半知菌亚门22个属、接合菌亚门2个属和鞭毛茵亚门1个属,表明胡杨根际真菌和内生真菌有着丰富的多样性.     

江西铜钹山自然保护区种子植物区系初步研究

对江西铜钹山自然保护区种子植物的组成、分布区类型和区系特征进行统计分析,结果表明:分布区的种子植物共计156科610属1 328种,其中裸子植物5科10属13种,被子植物151科600属1 315种。含20种以上的科共16科,单、寡种属共554属,其区系地理成分复杂,在植物区系组成中具有重要作用。在科的分布区类型中,泛热带分布科居多,共68科;其次是世界分布科,共29科。在属和种的分布区类型中,都是泛热带分布最多,北温带分布次之,说明该区的植物区系有明显的过渡性质。     

海口市重点药用植物调查

为海口市重点药用植物资源的科学保护及合理开发利用提供科学依据,采用野外调查、标本采集与查阅资料相结合的方法,对该地区重点药用植物资源的分布、生境和储量进行调查。结果表明:该地区重点药用植物共有40科66属69种,资源较为丰富。其中,蕨类植物2科2属2种,裸子植物1科1属1种,被子植物37科63属66种;国家重点种类48种,海南地方重点种类21种,分别占海口市重点种类的69.57%和30.43%。重点药用植物在调查样方中的出现频率差异较大,入药部位多样。     

林麝躯干骨的解剖

运用大体解剖学方法,对3头林麝的躯干骨进行了研究,并与牛羊躯干骨作了比较。林麝躯干骨的主要特征是:胸椎为13～14个,第2～10胸椎棘突高度比较接近。腰椎为6～7个,腰椎的横突朝向前腹外侧。腰椎的棘突宽,前后棘突顶端相互重叠。荐椎为5个,愈合成荐骨。荐骨后端显著变宽。荐骨翼发达。尾椎为7个,无脉管沟。肋为13～14对,其中,胸骨肋为8对。胸骨由7个节片构成,均呈上下压扁状。     

辽宁省烟田土壤真菌初步研究

对从辽宁省11个县区采集的267份烟田土壤进行分离,共鉴定出真菌20个属26个种。其中接合菌4属2种,有丝分裂孢子真菌16属24种。优势种群为:青霉属Penicillium、曲霉属As-pergillus、木霉属Trichoderma和镰孢霉属Fusarium,其数量分别占分离真菌总数量的31.87%,25.41%,17.43%和14.39%     

果蔬重要实蝇属的分布、危害与形态特征比较研究

阐述了果实蝇属、寡鬃实蝇属、小条实蝇属、按实蝇属、绕实蝇属5个检疫性害虫果蔬实蝇属的地理分布、寄主及其主要形态特征,比较了各个属形态特征的异同,并提出了一些防治果蔬重要实蝇属的措施。     

4种鸣虫染色体核型研究(直翅目:蝗总科)

采用常规压片法对网翅蝗科的隆额网翅蝗(Arcyptera coreana Shiraki)、宽翅曲背蝗[Paracyptera microptera meridionalis(Ikonnikov)]及斑翅蝗科的赤翅蝗(Celes skalozubovi Adelung)和鼓翅皱膝蝗[Angaracris barabensis(Pall.)]4种具有鸣叫行为的蝗虫进行染色体核型研究。结果表明:4种蝗虫性别决定机制为XO型,网翅蝗科2种蝗虫染色体数目:2n(♂)=20+XO=21。斑翅蝗科2种蝗虫染色体数目:2n(♂)=22+XO=23。4种直翅目鸣虫的染色体核型在较高的分类阶元中彼此间存在较为稳定的差异性;同科的蝗虫体现较近的亲缘关系,染色体核型存在较大的共性(染色体数目及染色体组式相同),然而在同一科下的不同属蝗虫间,染色体绝对长度及相对长度存在一定差异;不同科间蝗虫的染色体组式、染色体绝对长度及相对长度均存在较大差异,因此蝗虫染色体的核型可用作科间、属间分类的依据。4种鸣虫染色体核型的相似性与形态学分类结果在一定程度上一致。     

永春牛姆林青钱柳天然林群落区系成分分析

对福建永春县牛姆林自然保护区中青钱柳天然林群落植物区系成分的研究,结果表明:该群落中共有维管束植物39科58属66种,其中双子叶植物27科43属49种,是区系成分的重要组成部分 该群落种子植物属共有11个分布类型,其中,热带成分有36属,占78 26%,其中又以泛热带分布、热带亚洲分布为多;温带成分6属,占13 04%;中国特有成分仅青钱柳一属