呼和浩特地区犬弓形虫病血清学调查

为了研究呼和浩特地区犬弓形虫感染情况,试验采用酶联免疫吸附试验(ELISA)对不同来源、不同性别、不同年龄试验动物的弓形虫抗体IgG与IgM进行检测.结果表明:酶联免疫吸附试验检测犬弓形虫抗体IgG与IgM 性率分别为12.4%和4.6%;不同来源犬的弓形虫感染率有显著差异,不同性别犬感染率无明显差异,随着年龄的增长I...     

北京地区犬猫弓形虫病流行病学调查

为初步调查北京地区犬猫弓形虫感染的流病学特征,用酶联免疫吸附试验(ELISA)方法检测2010年5月至2011年4月采集的家养犬猫、流浪猫血清样本.其中,家养犬血清样本1876份,家养猫血清样本561份,检测发现,家养犬动物弓形虫IgG抗体阳性率24.9％;家养猫弓形虫IgG阳性率21.2％;同时检测流浪猫样本201份,阳性率30.3％.家养犬弓形虫血清抗体阳性率不同季节间差异显著(P＜0.05),夏季最高,为30.0％,家养猫弓形虫血清抗体阳性率不同季节间无显著差异(P＞0.05).不同性别犬猫弓形虫血清抗体阳性率无显著差异(P＞0.05).随着年龄增长,犬猫弓形虫抗体阳性率均有明显增长.对25例弓形虫抗体阳性家养犬病例和37例弓形虫抗体阳性家养猫病例进行了回访调查,结果发现,该62例动物主人的弓形虫检测结果均为阴性.     

厦门市宠物医院犬猫弓形虫病血清学调查

为了解厦门市宠物医院犬猫弓形虫感染情况,2019-2020年采集犬血清235份、猫血清191份,进行弓形虫病血清学调查.经酶联免疫方法检测发现,厦门地区犬弓形虫抗原和IgG抗体阳性率为4.68％,猫弓形虫抗原阳性率为5.24％,IgG抗体阳性率为4.71％.对不同健康状态、生活环境、性别和年龄进行比较分析,发现犬猫弓形...     

北京地区犬猫弓形虫病血清学调查

比较了酶联免疫吸附试验（ELISA）和乳胶凝集试验对犬猫进行龚地弓形虫（Toxoplasma gondii）抗体检测的结果.在66只犬猫的血清中,ELISA方法检测的阳性率为24.0%,乳胶凝集试验阳性率为21.0%,二者的检测结果差异不显著（P＞0.05）.后采用ELISA方法对北京地区家养的159只犬和128只猫进行了弓形虫IGg抗体的检测,共有21只犬（13.21%）和18只猫（14.06%）检测结果为阳性.1岁以下犬（3.70%）、猫（4.35%）低于1至3岁犬（11.29%）和猫（6.98%）的感染率.3～6岁犬猫的感染率分别是27.27%和16.22%,6岁以上犬（30.0%）、猫（32.0%）的感染率最高.不同性别之间感染无明显差异（P＞0.05）.     

内蒙古部分地区犬猫弓形虫病流行情况调查

为了调查内蒙古呼和浩特地区犬猫弓形虫病的流行情况,采用酶联免疫吸附试验和胶体金免疫层析法对呼和浩特地区共254只犬猫进行弓形虫抗体检测,采用胶体金免疫层析法对阳性感染犬猫进行抗原检测.结果显示,利用酶联免疫吸附试验检测犬的弓形虫阳性率为12.6%,猫为10.3%;利用胶体金免疫层析方法检测犬的弓形虫阳性率为11.4%,猫为9.2%;利用胶体金免疫层析方法对阳性犬猫进行抗原检测,检出率分别为23.8%和33.3%.调查表明,不同来源犬猫的弓形虫感染率差异显著,因此,加强对散养及流浪犬猫的管理是非常必要的.同时建议加强血清学监测,以便及时有效地采取预防措施.     

重庆地区猪弓形虫血清流行病学调查

为了解重庆地区猪弓形虫的感染情况,从重庆市合川、万州、北碚等17个区（县）采集猪血样,分离血清,用酶联免疫吸附试验（ELISA）检测弓形虫抗体。结果表明,受检的283份血清样本,弓形虫抗体阳性171份,阳性率60.42%。其中,以石柱、巴南、合川、武隆、万州5个区县的猪弓形虫抗体阳性率较高,分别为95.24%、83.33%、80%、75.76%和71.43%。提示重庆市多数地区存在猪弓形虫感染,且感染程度较为严重。     

牛新孢子虫病和弓形虫病的流行病学调查

为了调查我国牛新孢子虫和弓形虫的感染情况，应用新孢子虫酶联免疫吸附试验（ELISA）和弓形虫间接血凝试验（IAT）分别检测了来自国内10个省（市、自治区）的262份乳牛、10份肉牛和40份水牛血清。结果显示，乳牛新孢子虫的抗体阳性率为17．2％，弓形虫的抗体阳性率为2．3％，没有检测到既有新抱子虫抗体又有弓形虫抗体的乳牛血清。各牛场所检乳牛血清的新孢子虫抗体阳性率在0～34．4％之间。在水牛和肉牛血清中未检测到新孢子虫抗体和弓形虫抗体。流产乳牛血清的新孢子虫抗体阳性率为20．2％，未流产乳牛为16．1％，其中血清抗体阳性乳牛主要在妊娠中晚期流产。各个年龄段乳牛血清的抗体阳性率差异不显著（P〉0．05）。不同妊娠胎次的乳牛血清抗体阳性率差异不显著（P〉0．05）。确认新孢子虫病在国内大部分牛场存在。     

河南信阳地区羊弓形虫病的血清流行病学调查

为了解信阳市羊弓形虫病流行情况,笔者于2014年11月-2015年12月,采用ELSIA方法对信阳市58家羊场的1 247份羊血清样品进行了检测分析。结果显示,羊弓形虫病阳性场有23个,占检测羊场总数的39.7%,样品总体阳性率为12.4%;不同县区、不同生长阶段羊群及不同月份间弓形虫抗体阳性率差异不显著。羊弓形虫感染已在信阳市普遍存在,建议有关部门做好该病的防控。     

武汉地区家犬弓形虫病流行病学调查

为调查武汉地区家犬弓形虫感染的流行病学特征,用间接血凝试验方法检测2013年3~12月采集的家犬血清样本共279份。检测结果表明,家犬弓形虫血清抗体阳性样本37份,阳性率为13.4%。家犬弓形虫血清抗体阳性率不同性别变化不明显,雌性13.0%,雄性13.4%;不同季节变化较明显,夏季最高,为14.0%,春季最低,为12.7%;随年龄增长呈上升趋势,3~5岁最高,为21.2%。     

银川市犬弓形虫病流行病学调查

通过对银川市4家宠物医院诊疗机构随机抽取的527份血清样本中进行弓形虫病的流行病学调查。结果显示,527份血清样品中共有阳性样品42份,阳性率为7.96%。     

Epidemiological survey of Neospora caninum infection in dogs from Romania

Native cathepsin-L cysteine proteinase (28 kDa) was purified from the excretory secretory products of Fasciola gigantica and was used for sero-diagnosis of F. gigantica infection in buffaloes by Dot-enzyme-linked immunosorbent assay (Dot-ELISA). The test detected F. gigantica field infection in these animals with a sensitivity of ～ 90%. No specific IgG antibody binding was displayed by sera obtained from 76 buffaloes considered to be Fasciola and other parasite-free by microscopic examination of faeces and necropsy examination of liver, rumen and intestine. Additionally, sera from 156 Fasciola-free buffaloes, yet infected with Gigantocotyle explanatum, Paramphistomum epiclitum, Gastrothylax spp., Strongyloides papillosus and hydatid cyst were all negative, indicating that F. gigantica cathepsin-L cysteine proteinase does not cross-react with these helminth parasites in natural infection of the host. The data indicated that cathepsin-L cysteine proteinase based Dot-ELISA reached ～ 90% sensitivity and 100% specificity with relation to above parasites in the detection of bubaline fasciolosis. The present Dot-ELISA diagnostic assay is relevant to the field diagnosis of F. gigantica infection in buffaloes.     

Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan

To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than that of B. gibsoni-negative dogs.     

Toxoplasmosis in Sheep: Epidemiological Studies in Flocks with Reproductive Loss from Toxoplasmosis

The epidemiology of toxoplasmosis was studied in 51 flocks with reproductive loss from the infection. Overt toxoplasmosis was diagnosed on 2–4 neighbouring farms on 6 occasions, involving a total of 15 flocks. In 14 of the 51 flocks the frequency of abortion was highest in a definite part or age group of the flock.Evidence was found that the source of infection may be confined to a particular part of an area or of a farm. There was apparently no transmission of Toxoplasma gondii from sheep with overt or latent toxoplasmosis to susceptible sheep. There were good reasons to believe that the parasite might have been transmitted via the silage in 1 of the flocks.     

福建省猪弓形虫病流行病学调查

用IHA方法对我省6个地区猪场520份血清进行了弓形虫病血清学调查,结果我省猪场不同程度的感染了弓形虫病,平均感染率为28%,感染率由20.0%~39.6%不等,这表明许多规模猪场虽未发生典型的弓形虫病临床症状,但该病对福建省猪群感染已达到一定程度,应该引起养猪业的高度重视。     

Epidemiological survey of Anaplasma platys and Ehrlichia canis using ticks collected from dogs in Japan

Detection of Anaplasma platys and Ehrlichia canis in ticks recovered from dogs in Japan was attempted using a species-specific nested PCR based on the 16S rRNA gene. A total of 1211 ticks recovered from 1211 dogs from all over Japan were examined for A. platys and E. canis. Four tick samples from Fukushima, Miyazaki and Kagoshima Prefectures recovered from four different dogs showed a positive reaction for A. platys. Although the four dogs did not show any clinical signs and no blood examination data were available, it is possible that A. platys has already been spread widely in Japan. No positive reactions were observed in any ticks examined for E. canis.     

伊川县猪弓形虫病血清学调查与分析

利用间接血凝试验,对河南省伊川县部分规模化猪场的897份血清样品进行了弓形虫抗体检测,以抗体效价≥1：64为阳性判定标准,结果发现,上述被检样品的总阳性率为17．7％（159／897）,母猪、保育仔猪、育肥猪的阳性率分别为27．9％（76／272）、158％（29／183）、12．2％（51／442）。阳性母猪、保育仔猪和育肥猪抗体效价的几何平均数分别为1：229．4、1：89．4、1：169．7。     

辽宁省警犬犬瘟热犬细小病毒和犬冠状病毒感染情况调查

犬传染病是危害养犬业健康发展的主要疾病,来自警犬方面的统计资料证实,全国每年免疫警犬的发病率为14％,其中传染病占60％以上,因传染病而死亡的犬超过死亡犬总数的80％。从近几年的临床实践和国外的有关报道来看,危害严重的犬病毒病主要有犬瘟热、犬细小病毒感染、犬传染性肝炎、犬冠状病毒病、犬副流感及狂犬病等6种疾病,