Preliminary field test of lyophilised contagious caprine pleuropneumonia vaccine

Fifty goats were immunised in the field against contagious caprine pleuropneumonia (CCPP) using a single dose (0.15 mg) of lyophilised, saponin killed Mycoplasma strain F38. Two months after vaccination, these goats together with 50 unimmunised control goats were challenged by contact with goats experimentally infected with CCPP. Twelve vaccinates and 14 controls died of diarrhoea due to salmonella infection during the first two weeks after challenge. The remaining immunised goats (38) with the exception of two goats which had elevated temperatures were protected from CCPP. Of the remaining 36 control goats, 30 contracted CCPP at a mean of 39 (+/- 14.3 SD) days after challenge and 27 of them died. These results show that the lyophilised killed F38 vaccine conferred 100 per cent protection against mortality and 95 per cent protection against clinical disease caused by Mycoplasma species strain F38.     

Treatment of contagious caprine pleuropneumonia

Goats that had been inoculated with the causal organism of contagious, caprine pleuropneumonia and treated, within a few days, with oxytetracycline or tylosin, were less severely affected than infected, untreated control goats. However, 20% of treated cases remained infective and were, presumably, capable of transmitting the infection.     

山羊传染性胸膜肺炎研究进展

山羊传染性胸膜肺炎(CCPP)是一种由山羊支原体山羊肺炎亚种引起的严重疾病,偶有绵羊和野生反刍动物感染,具有超高的发病率和病死率。CCPP的病理特征为病变肺呈葡萄酒色,可完全肝化,通常伴随纤维素性胸膜炎,胸腔积聚大量积液。CCPP可通过血清学试验、DNA扩增(PCR、RFLP、杂交)和测序进行诊断。目前,抗生素疗法是CCPP的主要治疗方式,但长期使用抗生素易产生抗药性。文章针对不同时期提出的诊断方法和治疗措施进行梳理总结,以期为CCPP的防控和治疗提供参考与新思路。     

A mycoplasma from acute contagious caprine pleuropneumonia in Kenya

Summary A mycoplasma was isolated from acute caprine pleuropneumonia in Kenya. The organism could be differentiated serologically from the known strains of mycoplasma with which it was compared. When the organism was inoculated into goats it caused pleuropneumonia which was readily contagious, and from which the organism could be reisolated.

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Resumen Se aisló un micoplasma de casos agudos de pleuroneumonia caprina en Kenia. Los organismos se diferenciaron serologicamente de otras cepas conocidas de micoplasma con las cuales se compararon. Cuando los organismos se inocularon en cabras, la enfermedad se reprodujo y el organismo causal pudo aislarse nuevamente de los casos clínicos.

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Résumé Un mycoplasme a été isolé d'un cas de pleuropneumonie aigu? de la chèvre au Kenya. Ce germe a pu être différencié sérologiquement des souches connues auxquelles il a été comparé. Lorsqu'il est inoculé à des chèvres saines, il provoque une pleuropneumonie réellement contagieuse et est réisolé à partir des lésions.

     

山羊传染性胸膜肺炎荧光定量PCR检测方法的初步建立

为实现山羊传染性胸膜肺炎(CCPP)快速检测,研究基于病原体山羊支原体山羊肺炎亚种的16S rRNA基因,设计合成特异性引物及探针,采用5'FAM-TAMRA3'标记物进行标记.结果显示,试验建立的CCPP-16S实时荧光定量PCR方法可特异性检测CCPP,产生特异性荧光信号,对相关其他病菌无法检出荧光信号;以CCPP...     

Comparative evaluation of different therapeutic protocols for contagious caprine pleuropneumonia in Himalayan Pashmina goats

Tropical Animal Health and Production - Therapeutic management of contagious caprine pleuroneumonia (CCPP) involves mostly the use of oxytetracycline followed by enrofloxacin and rarely tylosin. In...     

A latex cryptococcal antigen agglutination test for diagnosis and monitoring of therapy for cryptococcosis

SUMMARY: A latex cryptococcal antigen agglutination test (LCAT) was performed on sera obtained during the first 14 days of treatment from 58 animals (46 cats, 9 dogs, 2 koalas and 1 long billed corella) with cryptococcosis. The same commercial kit was used for all samples, and most serum samples were treated with pronase before testing. Sera from all 58 cases tested positive with the qualitative LCAT protocol (using undiluted sera), while sera from all 26 cats without cryptococcosis tested negative. Titres determined using the quantitative protocol ranged from 1 to 131 072 (median titre between 2048 and 4096), with 57 of 58 cases (including all 8 animals that presented for neurological signs) having titres 2 and thus considered positive according to the manufacturer's recommendations. The LCAT titre was positively correlated with disease severity (r = 0.4169; P = 0.0011), and patients with disseminated skin and/or lymph node involvement had significantly higher titres than those that did not (P = 0.0157). The presence of neurological signs, the species of the patient, concurrent viral disease (in cats) and the biotype of the isolate had no significant association with the LCAT titre. Cats that died of active cryptococcosis despite treatment did not have significantly higher titres (P = 0.3010) than those that responded to treatment. Sequential LCAT determinations obtained in 37 patients during treatment provided a useful quantitative indication of clinical progress, although the decline in titre lagged somewhat behind clinical improvement. Generally, the antigen titre declined by 2 to 4 fold per month during successful therapy. Although there are insufficent data to make unequivocal recommendations, we suggest that either antifungal therapy be continued until the LCAT titre declines to less than 1, or therapy be discontinued after a 32 fold or greater reduction in titre, with periodic monitoring of the serum antigen titre. Treatment of serum samples with pronase substantially increased the sensitivity of the LCAT.     

Effect of danofloxacin (Advocin A180) on goats affected with contagious caprine pleuropneumonia

The efficacy of danofloxacin (Advocin A180) was evaluated for the treatment of contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae. Ten healthy Angora goats, confirmed free of CCPP, were exposed to clinically affected animals from a natural outbreak in Thrace, Turkey. After 14 days exposure, 8 goats showed pyrexia ( > or = 41 degrees C). Shortly after, the Angora goats were divided randomly into two groups. Five of these were injected with danofloxacin (6 mg/kg subcutaneously), which was repeated after 48 h; the five remaining animals received saline. Goats were monitored clinically and blood samples were collected for serology. Animals with severe disease were withdrawn from the trial. Goats completing the study were euthanized at day 42. Lung tissue and bronchial fluid were collected for mycoplasma isolation. All danofloxacin-treated goats showed resolution of clinical disease by the end of the trial. Two saline-treated goats failed to complete the study owing to CCPP. Danofloxacin-treated goats showed fewer lung lesions and had significantly lower combined clinical scores than saline controls (p < 0.001). Danofloxacin was found to be highly effective in the treatment of CCPP in goats.     

山羊传染性胸膜肺炎研究进展

山羊传染性胸膜肺炎是由山羊支原体山羊肺炎亚种引起的一种山羊常见的高度接触性呼吸道传染病,有传染快速,发病率、死亡率高的特点,给山羊养殖业带来巨大损失。本文从病原学、流行病学、临床症状以及诊断与防控方面对山羊传染性胸膜肺炎进行综述,帮助读者更加全面、深入的了解本病,以期为山羊传染性胸膜肺炎的诊断和防治提供新思路。     

A rapid latex agglutination test for detection of leptospiral antibodies

A rapid semi-quantitative latex agglutination test (LAT) has been standardized for the detection of leptospiral antibodies in serum samples of man and animals. The efficacy of the LAT was compared with the plate enzyme linked immunosorbent assay (ELISA). A total of 276 human serum samples were analyzed by both LAT and ELISA and percentage positives were 84.8 and 85.9%, respectively. Similarly, of 65 animal samples tested, 63.1 and 69.2% positivity were observed in LAT and ELISA, respectively. Even though the ELISA test was slightly more sensitive than LAT, the rapidity, simplicity and economics of the LAT were found to fulfill the requirements of a screening test for leptospiral antibodies.