The effect of rate and timing of phosphate fertilizer on the yield and phosphate off take of grass grown for silage at moderate to high levels of soil phosphorus

A series of twenty-one trials was undertaken during 1985–1988 to investigate the effect on lowland silage crops of 0, 40 or 80 kg ha⁻¹ phosphate (P₂O₅) fertilizer applied as triple superphosphate (46% P₂O₅) in the autumn, spring or as a split application (spring and after first cut). All sites had moderate to high sodium bicarbonate-extractable soil phosphorus contents (18–34 mg P 1⁻¹ in air-dried soil).
Significant yield responses were obtained at eight of the forty-two individual cuts (two cuts per site). When meaned over all sites, spring or autumn applied phosphate increased dry matter yield at both first and second cut (mean total increase 0·32 t ha⁻¹), though the effect was significant only at the highest phosphate rate. The time of phosphate application had no overall effect on yield. Herbage P concentration and phosphate off take were significantly increased at both cuts by both rates of phosphate. Generally, the most recent application had a significantly greater effect than other timings. These findings indicate that DM yield responses to freshly available phosphate can occur on soils of moderate P status.     

The effect of timing of slurry application on the microflora of grass, and changes occurring during silage fermentation

Cattle slurry (50 m³ ha^?1 equivalent to 68 kg N ha^?1) was applied to grassland plots 70 d (early application) and 34 d (late application) before ensiling and the retention and survival of slurry and epiphytic micro-organisms on the growing herbage were examined and compared with those on herbage from corresponding fertilizer-treated plots. The populations of lactic acid bacteria, enterococci and enterobacteria on herbage increased dramatically after slurry application. Thereafter, numbers of lactic acid bacteria declined, although they were always higher than on untreated herbage. Number of enterobacteria also declined but were higher on chopped grasses at ensiling [10⁶ colony-forming units (CFU) g^?1 fresh matter (FM)] than they were on hand-cut. unchopped herbages at all previous sampling times. Clostridia numbers were lowest on untreated and highest on slurry-treated herbage, particularly after the late application (>10³ CFU g^?1 FM). Herbage was harvested 70 d and 34 d after slurry application, chopped and ensiled in laboratory silos. All herbages, irrespective of treatment, had low dry matter (DM) values (ranging from 149 to 170 g kg^?1 FM) and fairly low water-soluble carbohydrate (WSC) concentrations (130 g kg^?1 DM or less). The initial rate of pH decline up to 4 d was most rapid in slurry-treated herbages, with all pH values falling to < 4. 5 by day 4 and remaining there until day 21. However, after 90 days the pH values of all silages had risen to > 4. 5. accompanied by a marked decline in lactic acid concentration. Lactic acid-fermenting Clostridia increased in numbers, reaching peak values of 10⁷ CFU g^?1 FM by day 21, remaining high until opening, and were probably responsible for increases in butyric acid levels in all silages, with the highest concentrations occurring in those prepared from slurry-treated herbages. The results suggest that, although some faecal lactic acid bacteria may have beneficial effects in the early stages of fermentation, Clostridia from slurry can survive on herbage for extended periods. The results indicate that the potential for growth of Clostridia in silage may be independent of source or size of the initial population even at tow pH, if other conditions are favourable.     

Effect of fertilizer nitrogen rate and timing on herbage production and nitrogen use efficiency for first-cut silage

Eight field-plot experiments were carried out on established grassland swards between 1984 and 1988 to examine the effects of date and rate of application of calcium ammonium nitrate (CAN) on herbage dry matter (DM) yield and apparent efficiency of nitrogen (N) use at first-cut silage.
CAN application significantly increased ( P <0 ·001) the mean yields of herbage and N uptakes by herbage in all experiments. Herbage yields were similar ( P > 0·05) with N rates of 100 kg ha⁻¹, 125 kg ha⁻¹ or 150 kg ha⁻¹ in five experiments but in the other three there were increases above 100 kg ha⁻¹. Date of N application had a significant effect on DM yield in three experiments; this effect was inconsistent for both single and split dressings. Lower production was associated with reduced uptake of N, a trend that primarily reflected lower DM yields and not wide herbage N content variation.
It is concluded that selection of the date on which to apply fertilizer N in early spring to obtain optimum herbage yields at first-cut silage often required little precision. The use of fertilizer N rates >100 kg ha⁻¹ should be questioned where there are likely to be appreciable quantities of available N derived from non-fertilizer sources.     

Predicting the digestibility of grass grown for first-cut silage

Meteorological factors influencing the temporal changes of digestibility of grasses in spring and early summer are discussed. Annual observations in England of the digestibility of three grasses are then related to the relevant meteorological variables using two multiple regression analysis methods. The resulting regression relationships are tested on independent data, and finally sets of regression coefficients are recommended for predicting from synoptic meteorological data the weekly progress of digestibility in different areas of England: a simplified set of regression relationships for predicting digestibility values a few days ahead which require forecasts of only temperature in order to be applied operationally is also given.     

The effect of fertilizer sulphur on grass production for silage

The effects of rate and pattern of fertilizer S application on herbage production from Perennial ryegrass-dominant swards at six potentially S-deficient sites in south-west Scotland were measured under a silage cutting regime (3 cuts year-1) with high fertilizer N application (300 kg ha⁻¹ year-1). Fertilizer S (as gypsum) was applied at annual rates from 0 to 48 kg ha⁻¹ as single spring applications before the first cut or split before each of the three cuts. The residual effect of S in the year following its application was assessed at one site.
Applied S increased total DM production at four of the six sites. These increases were restricted to the second and/or third cuts and were generally in the range 10-30% greater than where no S was applied. Timing of S application was not important in influencing annual DM yield. The residual effect of gypsum in the year following application was small.
The supply of available soil S is limiting herbage production in certain areas and soil types of south-west Scotland. Areas of S deficiency are likely to increase in the future and need to be identified more precisely than they are at present.     

The effect of glucose oxidase on the preservation of grass silage

Five experiments were carried out to investigate the effects of glucose oxidase (GO) on the preservation of grass silage in the absence of micro-organisms. In the first experiment, GO was tested in a 3% glucose solution. At the level of 1190 U GO l^-1 the pH value decreased to 3·94 in 1 h and to 3·02 in 24 h. At the level of 11·9 U l^-1 the pH decreased to 3·45 in 24 h.
The second experiment was performed by packing chopped grass into glass jars, sterilizing in an autoclave and adding GO. One day later the pH value had decreased to 4·65; 1·60% gluconic acid was found in the dry matter.
In the other three experiments grass was sealed in polythene bags and sterilized by gamma-irradiation. GO was found to cause a faster decrease in pH. Removing air from the silage by compaction decreased the GO activity. The addition of cellulase delayed the GO functioning for one day. Lactobacillus inoculant had no effect. Evidence was obtained that GO may affect the profile or activity of the original microflora of grass silage.     

The effect of dry matter content and silage additives on the fermentation of grass silage on commercial farms

A total of 1713 samples of silage from commercial farm silos were analysed to investigate the effect of dry matter (DM) content and chemical additives on fermentation as measured by ammonia-N concentration and pH, Increasing DM content without additive use had a major beneficial influence upon fermentation. When silage DM contents were greater than 260 g kg^-1 83% of silages were well fermented, with average ammonia-N concentrations of 94 g (kg total N)^-1 and pH 4.36. With diminishing DM concentration the proportion of well fermented silages declined. In the DM range 220-260 g kg^-1 67% of silages were well fermented with ammonia-N concentrations of 125 g (kg total N)^-1 and pH 4.30, in the DM range 180-220 g kg^-1 48% were well fermented with ammonia-N at 151 g (kg total N)^-1 and pH 4.38 and with DM below 180 g kg^-1 no silages were well fermented with ammonia-N concentration of 252 g (kg total N)^-1 and pH 4.84. The benefit of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of increased DM concentration. Below DM concentrations of 180 and 220 g kg^-1, the only benefit was that formic acid decreased ammonia-N to 151 g (kg total N)^-1 and pH to 4.32 compared with 163 g (kg total N)^-1 and pH 4.43 for untreated silages. Within the DM range 220-260 g kg^-1 formic acid decreased ammonia-N level to 104 g (kg total N)^-1 and pH to 4.07, and there was a slight benefit from using sulphuric acid/formalin which decreased ammonia-N to 117 g (kg total N)^-1 and pH to 4.23 compared with 125 g (kg total N)^-1 and pH 4.27 for untreated silages. Above 260 g DM kg^-1 both formic acid and sulphuric acid/formalin provided a small but consistent decrease in ammonia-N and pH compared with untreated silages. Chemical additive use conferred no other benefit when compared with untreated silage. Calcium formate/sodium nitrite mixtures and acid mixture use provided no benefit in fermentation compared with untreated herbage.     

Effect of forage matting on rate of grass drying, rate of silage fermentation, silage intake and digestibility of silage by sheep

The effects of forage matting on rate of grass drying and silage fermentation, digestibility, and intake were examined using perennial ryegrass swards. Treatments compared were: forage mats, where grass was processed through a laboratory scale macerator prior to matting and wilting to 228 g dry matter (DM) kg^?1 (FM treatment); unconditioned grass which was direct ensiled at 163 g DM kg^?1 (DE treatment); unconditioned grass which was wilted for the same period as FM to 213 g DM kg^?1 (UC treatment); unconditioned grass which was wilted to 234 g DM kg^?1 (UC₂₅, treatment). All forages were dried on black plastic sheeting. For each treatment a total of approximately 80 kg grass DM was ensiled in seven 290 I plastic bins for 136 d prior to feeding to wether sheep. A further total of 14 kg grass DM from each treatment was ensiled in twenty-one plastic pipes (152 mm diameter, 762 mm long) to give a total of 84 pipes. Rate of silage fermentation was determined by destructively sampling pipes following 1, 2, 4, 6, 13, 20 and 50 d of ensilage. Over the mean wilting period of 6·9 h, grass from the FM treatment dried significantly faster (P < 0·001) and required less solar energy per unit of moisture loss than unconditioned grass. The rate of grass drying was highly correlated with solar radiation. The FM treatment did not influence the rate or extent of silage fermentation. The intakes and digestibilities of FM, UC and UC₂₅ were not significantly (P < 0·05) different from each other but were higher than for the DE treatment (P < 0·05 for digestibility and NS for intake). In Northern Ireland it is unlikely that there will be sufficient solar radiation to allow forage mats to be made, wilted to a level to prevent effluent production and harvested within one working day. Further work is required to optimize mat-making technology for more rapid drying and to determine the effect of adverse weather on nutrient losses from mats.     

The effect of frequency of harvesting grass for silage on the intake and performance of beef cattle

Two randomized-block experiments were conducted to examine the effects of frequency of harvesting grass for silage on the intake and performance of beef cattle. In both experiments swards of S24 perennial ryegrass were harvested at 63-, 49- and 38-d intervals throughout the growing season. Grass harvested before and after 10 July was ensiled separately and termed spring and autumn silage respectively. In Experiment 1 the silages were offered ad libitum either unsupplemented or supplemented with 2 kg barley per head daily to eighty-four Hereford-cross weaned, single-suckled steer calves of mean initial live weight 284 kg, in a 3 × 2 × 2 factorial design. Silage dry matter (DM) intakes and liveweight gains for the silages harvested at 63-, 49- and 38-d intervals were 4.80, 0.72; 4.49,0.76; and 4.62,0.78 kg d⁻¹ for the spring silages and 4.69, 0.67; 4.59, 0.85; and 4.55,0.86 kg d⁻¹ for the autumn silages respectively. There was no significant interaction between frequency of harvesting and concentrate supplementation. In Experiment 2 the silages were offered ad libitum and unsupplemented to forty-two Hereford-cross weaned, single-suckled steer calves of mean initial live weight 240 kg, and forty-two Hereford-cross store cattle of mean initial live weight 356 kg, in a 3 × 2 × 2 factorial design. Silage DM intakes and liveweight gains for the silages harvested at 63-, 49- and 38-d intervals were 4.94,0.49; 5.69,0.80; and 619,0.93 kg d⁻¹ for the spring silages and 5.50, 0.61; 5.57, 0.72; and 505, 0.65 kg d⁻¹ for the autumn silages respectively. There were no significant interactions between frequency of harvesting and type of animal. It is concluded that a cutting interval of 49 d commencing on 21 May is likely to be optimum for this type of sward except under exceptional weather conditions.     

The effects of rate and timing of application of fertilizer nitrogen in late summer on herbage mass and chemical composition of perennial ryegrass swards over the winter period in Northern Ireland

A small‐plot experiment was carried out in Northern Ireland on a predominantly perennial ryegrass sward over the period July 1993 to March 1994 to investigate the effect of timing and rate of fertilizer nitrogen (N) application on herbage mass and its chemical composition over the winter period. Eighty treatment combinations, involving four N fertilizer application dates (28 July, 9 and 30 August and 20 September 1993), four rates of N fertilizer (0, 30, 60 and 90 kg N ha^?1) and five harvest dates (1 October, 1 November, 1 December 1993, 1 February and 1 March 1994), were replicated three times in a randomized block design experiment. N application increased herbage mass at each of the harvest dates, but in general there was a decrease in response to N with increasing rate of N and delay in time of application. Mean responses to N applications were 13·0, 11·5 and 9·5 kg DM kg^?1 N at 30, 60 and 90 kg N ha^?1 respectively. Delaying N application, which also reduced the length of the period of growth, reduced the mean response to N fertilizer from 14·3 to 7·4 kg DM kg^?1 N for N applied on 28 July and 20 September respectively. Increasing rate of N application increased the N concentration and reduced the dry‐matter (DM) content and water‐soluble carbohydrate (WSC) concentration of the herbage but had little effect on the acid‐detergent fibre (ADF) concentration. Delaying N application increased N concentration and reduced DM content of the herbage. The effect of date of N application on WSC concentration varied between harvests. A decrease in herbage mass occurred from November onwards which was associated with a decrease in the proportion of live leaf and stem material and an increase in the proportion of dead material in the sward. It is concluded that there is considerable potential to increase the herbage mass available for autumn/early winter grazing by applying up to 60 kg N ha^?1 in early September.     

Effect of extent and rate of wilting on nitrogen components of grass silage

Wilting grass prior to ensiling generally increases the dry matter (DM) intake but the effect of wilting on animal performance is still poorly understood. There is a need to improve understanding of the effects of wilting on the nutritional components and chemical composition of grass silage. This study focused on the effects of the extent and rate of wilting on N components of grass silage. Meadow grass was wilted to four DM contents (200, 350, 500, 650 g kg^?1) at two different rates (fast, slow), creating a total of eight silages. Crude protein (CP) fractions were measured using the Cornell Net Carbohydrate and Protein System. Utilizable CP at the duodenum (uCP), a measure of feed protein value, was estimated using the modified Hohenheim gas test. Ruminally insoluble, undegraded feed CP (RUP) was measured using an in situ technique. Amino acid (AA) composition prior to and after rumen incubation was also investigated. Utilizable CP at the duodenum, RUP and true protein fractions B2 and B3 were increased by rapid wilting and high DM content (DM > 500 g kg^?1), although the increase with DM was only mild for uCP, probably due to lower ME content in the DM‐650 silages. Non‐protein‐N decreased with increasing DM and rapid wilting. The higher RUP content from both DM‐650 silages leads to a higher total AA content after rumen incubation. Treatment also influenced the AA composition of the ensiled material, but the AA composition after rumen incubation was similar across treatments. Rapid and extensive wilting (DM > 500 g kg^?1) improved protein value and reduced CP degradability. Increased uCP may result in higher milk protein yield, while reduced degradability may reduce N lost from urinary excretion. The primary effect of wilting on post‐ruminal AA supply from RUP appeared to be quantitative, rather than qualitative.     

The effects of nitrogen and phosphorus fertilizer application on herbage yield of natural pastures

This study was carried out in a natural pasture in Van province of Turkey between the years of 2004 and 2005 to determine the effects of nitrogen and phosphorus fertilization on herbage yield. The study was performed in randomized block with three replications. 0, 4, 8, 16 kg da(-1) nitrogen and 0, 6, 12 kg da(-1) phosphorus applications were examined. The effects of different nitrogen and phosphorus fertilizer applications on plant height, green herbage and crude protein yield were significant for both years. Depending on the increasing nitrogen and phosphorus applications, significant increases were in green herbage, hay and crude protein yields. According to results, differences between 8 and 16 kg da(-1) doses of nitrogen and 6 and 12 kg da(-1) doses of phosphorus applications were insignificant. In the first year, in terms of nitrogen applications, the highest green herbage, hay and crude protein yield (1423.1, 263.3 and 29.2 kg da(-1)) were obtained from 8 kg N da(-1), in the second year, the highest values (1426.1, 602.7 and 67.8 kg da(-1)) were obtained from 16 kg N da(-1) application. As for the phosphorus applications, in the first year, the highest green herbage yield was 1142.2 kg da(-1) at the 6 kg P2O5/da application, hay and crude protein yields (218.2, 23.1 kg da(-1)) were recorded from 12 kg P2O5/da application. In the second year, the highest green herbage, hay and crude protein yields were (1335.8, 549.6 and 66.1 kg da(-1)) determined at the 12 kg P2O5/da application.     

The effect of cutting systems on the yield of herbage from a small-plot experiment

Plots of an established perennial ryegrass sward (cv. Talbot) fertilized with 100 or 300 kg N ha⁻¹ were harvested with one of three plot mowers; an Agria with 38-mm finger spacing and an Allen Mayfield with 38-mm finger spacing or 78-mm finger spacing. The choice of mower was arranged factorially with four post-harvest treatments: no post treatment; a second (cleaning) cut using the same mower and cutting in the same direction as the first (sampling) cut; a cleaning cut using the same mower, but in the opposite direction to the sampling cut; and a cleaning cut using a rotary mower. Sward productivity as organic matter (OM) was measured over four harvests at 6- to 7-week intervals. The Agria mower resulted in 12·3 and 15-9% more OM yield than the Allen Mayfield fitted either with knife-bars at a similar spacing, or with wider-spaced fingers, respectively. Yield of OM in the subsequent cut was reduced when a cleaning cut was taken; particularly when it was taken in the opposite direction to the sampling cut or when using a rotary mower.     

Effect of sward height on the fermentability coefficient and chemical composition of Guinea grass silage

There is a high correlation between sward height and pasture sward structure. Therefore, in tropical grasslands, taking sward height into account has been a much better strategy in rotational stocking management than considering pre‐defined days of growth. Similarly, sward height could be used to determine the moment when tropical grasses present the best ensilability parameters. This study aimed to identify the sward height at which Panicum maximum cv. Mombaça (Guinea grass) provides the highest fermentability coefficient (FC) and to define the combination of additives that best improves the chemical composition of silage. Two trials were carried out in Selvíria, MS, Brazil, from 2015 to 2016. The first year was used to identify the highest FC, and the second year was used to identify the best combination of eight additives (citrus pulp [CIP], homofermentative and heterofermentative LAB, their combinations and control). Statistical analyses were performed using SAS (p < .05), and one contrast was defined as silage with CIP vs. silage without CIP. The height of 130 cm resulted in the highest FC (31.01). Silages inoculated with CIP had better quality than silages without CIP, due to the high crude protein (8.3 vs. 7.3% DM), DM recovery (98.6 vs. 93.3% DM), low pH (3.92 vs. 4.91) and NH₃‐N values (2.49 vs. 14.73% total N). Sward height is a consistent parameter for determining the time of ensiling Guinea grass, and the inclusion of CIP is necessary to raise the silage quality.     

Negative effects of winter- and spring-applied cattle slurry on the yield of herbage at simulated early grazing and first-cut silage

Experiments were earned out on seven UK sites during 1985–1988 to evaluate the importance of negative effects (smoethering and scorch) of winter and spring-applied cattle slurry on grass yield, assessed at the time of early grazing or first-cut silage. Slurry application has both positive and negative effects on herbage growth. Positive effects, usually associated with herbage yield increases, derive from the nutrient value of the slurry. Negative effects, often resulting in herbage yield decreases, are caused by smothering and/or scorch. The net effect on herbage depends on the balance between these positive and negative effects. These experiments were designed to isolate the negative effects of slurry whilst attempting to minimize the positive effects. Yield reductions of up to 20% for early grazing and 5% for first-cut silage were recorded, following applications of cattle slurry in February and March. Earlier applications smaller yield reductions. The threshold for the occurrence of yield reduction was estimated at 3–4 t ha^?1 of slurry solids applied, at 6% solids content. In practice this would mean an application of 50–65 m³ ha^?1 at 6% dry matter, which would supply 150–200 kg ha^?1 total nitrogen, based on typical slurry analyses. Within the limits of application rates tested in these experiments, yield depression tended to increase with increasing rate of slurry applied above this threshold. Although the results suggest that the effects are more likely to occur following spring applications of slurry, earlier (autumn or winter) applications would increase the risk of losses of slurry nitrogen by leaching and denitrification. These results, therefore, confirm current advice in the UK that, where possible, cattle slurry is best applied to grassland in early spring according to the fertilizer needs of the crop. High rates of application should be avoided after early March for cutting areas and, more especially, for early grazing.     

Enzymes as silage additives 2. The effect of grass dry matter content on silage quality and performance in sheep

Eight grass silages were made from two contrasting varieties of perennial ryegrass, four silages each from a late-cut early-maturing (high dry matter, HDM) variety and from an early-cut, late-maturing (low dry matter, LDM) variety. The grass was ensiled without additive (untreated), or with formic acid, or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2) for a period of 130 d.
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d⁻¹) of untreated and enzyme-treated silages were significantly ( P <0·01) lower at both digestibilities compared with formic acid-treated silages (LDM: untreated, 982; formic, 1069; enzyme 1, 868; enzyme 2, 937; HDM: untreated, 931; formic, 1027; enzyme 1, 943; enzyme 2, 914). The organic matter, carbohydrate and nitrogen digestibility coefficients of LDM silages were significantly ( P <0·001) higher than those of HDM silages. There were no significant differences in any component digestibility related to silage additive.
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species.     

The effect of partial replacement of grass silage with fresh grass on rumen fermentation characteristics and rumen outflow rates in cattle

An experiment was designed to examine the effects of partially replacing extensively fermented grass silage with varying proportions of fresh grass (0, 0–33, 0–67 and 100) on rumen fermentation, degradation of dry matter (DM) and rate of outflow of liquid and particulate phases from the rumen with four mature Limousin steers. The fresh grass had a higher pH and water-soluble carbohydrate and lower ammonia-N and lactic acid concentrations than the silage. Partial replacement of silage with fresh grass resulted in a reduction in rumen ammonia concentration, and in the proportion of rumen propionate, i-butyrate and n -valerate and an increase in the proportion of rumen acetate and in both die particulate and liquid outflow rates from the rumen. These changes in rumen fermentation parameters could account for increases in animal performance in situations in which grass silage is partially replaced with fresh grass.     

Influence of cutting regime and fertilizer application on the botanical composition,yield and nutritive value of herbage of wet grasslands in Central Europe

The changes in dry matter (DM) yield, botanical composition and nutritive value of herbage to ruminants of two wet grasslands, Arrhenatherum elatius grassland (Experiment 1) and a Molinia caerulea fen meadow (Experiment 2), in which a range of cutting and fertilizer treatments were imposed in 1999, were assessed after 4–7 years of treatment imposition. Both experiments had a split‐plot design with four replicates. In Experiment 1 the three main‐plot cutting treatments were two cuts with a delayed first cut, three cuts and four cuts during the growing season of each year. In Experiment 2 the cutting treatments were two cuts with a traditional harvest time, two cuts with a delayed first cut and three cuts. The four sub‐plot fertilizer treatments were an unfertilized control, application of a phosphorus and potassium (PK) fertilizer, application of a nitrogen (N) and PK fertilizer to the first cut only (N₁PK) and application of PK plus N applied to each of two, three or four cuts (N_c PK). Application of fertilizer influenced yield and botanical composition of herbage more than the cutting treatments while the opposite occurred for nutritive value of the herbage. Application of fertilizer increased the proportion of tall grasses in Experiment 1 and forbs in Experiment 2. The proportion of Equisetum palustre, present only in Experiment 1, was reduced from 0·33 to less than 0·01 by increased cutting frequency together with the NPK fertilizer treatments. In Experiment 1 diversity of vascular plants was negatively affected only by the four‐cuts treatment while on both wet grasslands other cutting and fertilizer application treatments had no effect. Changes in DM yield of herbage caused by the cutting and fertilizer application treatments were similar for both vegetation types with DM yield increased significantly by fertilizer application but only slightly or not reduced by increasing the cutting frequency. Nutritive value of herbage was positively correlated with cutting frequency and was most influenced at the first cut.     

The effects of wilting grass before ensiling on silage intake

Herbage, predominantly perennial ryegrass (Lolium perenne) grown in Northern Ireland, was harvested at four dates from June to October 1996 (H1, H2, H3 and H4). At each harvest approximately one-fifth of the grass harvested was artificially dried and pelleted (G). The remainder of the grass was either wilted for 28–52 h (W), depending on the weather conditions, or ensiled directly, i.e. unwilted (UW). Within the W and UW treatments an inoculant or formic acid additive was applied to the herbage before ensiling. After a minimum ensiling period of 10 weeks, sixty steers, mean initial live weight 432 (s.d. 37) kg, were offered the twenty forages in a four-period partially balanced changeover design experiment. Each period was of 2 weeks’ duration. Dry-matter (DM) intakes were recorded daily, with intakes in the second week of each period used in the statistical analysis of the data. The digestibility of each of the forages was also determined in vivo using four castrated male sheep per silage. Wilting increased the DM content of the silage and the pH, the largest increase in DM content occurring at the second harvest. On average, wilting proportionally increased silage DM intake by 0·21 compared with the unwilted silage (P < 0·001), but the intake of the wilted silage was not significantly different from that of the artificially dried and pelleted grass (P > 0·05). The intake of the wilted silage was higher than that of the unwilted silage at each harvest, the proportional increases being 0·22 (P < 0·001), 0·41 (P < 0·001), 0·19 (P < 0·001) and 0·05 (P > 0·05) at harvests H1, H2, H3 and H4 respectively. Treatment of the grass with formic acid before ensiling resulted in a proportional increase in silage intake of 0·08 compared with the inoculant-treated silage (P < 0·05). Compared with the inoculant-treated silage, formic acid increased silage intake by 0·08, 0·02, 0·14 and 0·10 at harvests H1 (P > 0·05), H2 (P > 0·05), H3 (P < 0·01) and H4 (P < 0·05). The results of this study indicate that the effect of wilting on silage intake varies across different harvests and additive treatments. The difference in response to wilting across different harvests is mainly a result of the prevailing weather conditions during wilting.     

The microbiological and chemical composition of silage over the course of fermentation in round bales relative to that of silage made from unchopped and precision-chopped herbage in laboratory silos

The composition of baled silage frequently differs from that of comparable conventional silage. A factorial experiment was conducted with three wilting treatments (0, 24 or 48 h) × three ensiling systems [unchopped grass in bales, unchopped grass in laboratory silos (LS), precision-chopped grass in LS] × six stages of ensiling to (i) confirm that the fermentation of unchopped grass in LS could be used as an adequate model for baled silage fermentation, (ii) quantify the differences between baled silage and silage made from precision-chopped herbage across a range of dry-matter contents and (c) quantify the fermentation dynamics within the various treatments. The onset of fermentation as evidenced by the accumulation of fermentation products and the decline in pH were slower ( P  < 0·05) in baled silage compared with silage made from precision-chopped herbage. Furthermore the pH ( P  < 0·001) and overall concentration of fermentation acids ( P  < 0·01) were lower while ammonia-N concentration was generally higher in baled silage, making it more conducive to the activities of Clostridia , Enterobacteria and yeast. Numbers of Enterobacteria were higher ( P  < 0·001) in baled silage in the early stages of ensilage and persisted in baled silage at the end of the storage period. The implications of a slower onset of fermentation in baled herbage are greater in farm practice, as the fermentation would be further restricted by a more extensive wilting of the herbage prior to ensiling.