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1.
Effect of dietary prebiotic inulin on histology,immuno‐haematological and biochemical parameters of Asian seabass (Lates calcarifer) 下载免费PDF全文
Sajjad Syed Raffic Ali Kondusamy Ambasankar Peter Ezhil Praveena Sambasivam Nandakumar Mohamed Saiyad Musthafa 《Aquaculture Research》2018,49(8):2732-2740
The present study was carried out to investigate the effect of supplementation of inulin on histology, immuno‐haematological and biochemical parameters of Asian seabass (Lates calcarifer) fingerlings with an initial average body weight of 7.14 ± 0.05 g. Inulin was supplemented at four different concentrations (control) 0, 5, 10, 15, and 20 g/kg in the L. calcarifer diet containing 400 g/kg protein and 90 g/kg lipid. At the end of the 60 days feeding trial, the absorptive surface area of the intestine and glycogen deposition in liver were increased in fish fed inulin supplemented diets. The immune parameters lysozyme, alternative complement pathway, superoxide dismutase and nitroblue tetrazolium assay showed significant (p < 0.05) difference between control and treatment groups. Haematological parameters showed that red blood cells, white blood cells, haemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration levels increased significantly (p < 0.05) in the fish fed with inulin supplemented diets. The analysis of biochemical parameters revealed that glucose, urea, cholesterol, triglyceride, aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase, and lactate dehydrogenase showed significant differences (p < 0.05) between control and treatments groups. No significant difference (p > 0.05) was observed for total protein among different treatments. The results of the study revealed that 15–20 g/kg inulin supplementation has a beneficial effect in the histology, immuno‐haematological, and biochemical parameters in L. calcarifer juveniles. 相似文献
2.
Ahmad Gharaei Mostafa Ghaffari Saeed Keyvanshokooh Reza Akrami 《Fish physiology and biochemistry》2011,37(3):485-493
In this paper, effects of dietary methylmercury (MeHg) on several blood biochemical parameters including GLU (glucose), LDH
(lactate dehydrogenase), AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase) and
cortisol were investigated in the Beluga sturgeon (Huso huso). Beluga juveniles were fed for 32 days on four diets containing MeHg (control: 0.04 mg kg−1; low: 0.76 mg kg−1; medium: 7.88 mg kg−1; and high 16.22 mg kg−1 treatment). Significant increases (P < 0.05) were observed in all biochemical parameters, except ALP levels, which decreased significantly (P < 0.05) compared to the control group with either dose- or time-dependent effects. These results suggest that long-term dietary
MeHg exposure may affect metabolic enzyme activity and glucose levels in Belugas. These findings provide useful information
for environmental and fishery officials to apply in future decisions for managing fish resources in Caspian Sea. 相似文献
3.
Sung Hee Jung Doo Saing Sim Mi-Seon Park QTae Jo & Yoon Kim 《Aquaculture Research》2003,34(14):1269-1275
Erythrocytes of olive flounder, Paralichthys olivaceus (Temminck et Schlegel), were treated with serial concentrations of formalin (37% formaldehyde) to investigate in vitro haemolysis and methaemoglobin formation. In addition, the short‐term toxicity of formalin concentrations of 0, 100, 212 and 300 ppm was also studied by clinical tests in which fish were subjected to 3‐h bath exposure. There was no haemolysis of fish erythrocytes exposed to formalin concentrations ranging from 31.3 to 2000 ppm. Methaemoglobin formation, however, was induced at concentrations greater than 500 ppm. Red blood cell count, haemoglobin, haematocrit, mean corpuscular haemoglobin concentration and percentage of immature erythrocytes were also markedly elevated in all formalin‐exposed groups (P<0.05). Formalin exposure also caused significant increases in alkaline phosphatase, aspartate aminotransferase, lactate dehydrogenase, potassium, chloride, magnesium and inorganic phosphorus (P<0.05). However, total protein decreased significantly in the formalin‐exposed groups (P<0.05). No significant differences in white blood cell count, mean corpuscular volume, mean corpuscular haemoglobin, albumin, glucose, total cholesterol, high‐density lipoprotein cholesterol, free cholesterol, alanine aminotransferase, calcium, creatinine and total bilirubin were observed in the formalin‐exposed groups (P>0.05). 相似文献
4.
Mass death of farmed brook trout Salvelinus fontinalis reared in raceway culture on a commercial fish farm was recorded in May. The condition was caused by natural infection with external signs of Flavobacterium columnare. Haematological analyses were carried out from peripheral blood from 10 fish from each group. Anaemia was characterized by a reduced erythrocyte count and lower haemoglobin levels; the values of mean corpuscular volume and mean corpuscular haemoglobin were higher and the values of mean corpuscular haemoglobin concentration were decreased. Clinical chemistry analyses in blood plasma indicated decreased levels of total protein, glucose and total calcium and an increase in the urea level. Among the four enzymes analysed, a catalytic activity reaching multiples of the normal level was found in alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase. The catalytic concentration of alkaline phosphatase was decreased. Electrophoretic analyses of the plasma protein indicated a reduced level of albumin. The moribund fish had a higher hepatosomatic index and their condition coefficient after Fulton and after Clark was also higher. 相似文献
5.
Effects of totally replacing dietary fish oil by linseed oil or soybean oil on juvenile hybrid sturgeon,Acipenser baeri Brandt♀×A. schrenckii Brandt♂ 下载免费PDF全文
C. Liu J. Wang Z. Ma T. Li W. Xing N. Jiang W. Li C. Li L. Luo 《Aquaculture Nutrition》2018,24(1):184-194
The growth performance, body composition, fillet fatty acid content, serum hepatic enzymes and postprandial changes in serum lipid metabolism of hybrid sturgeon (70.8 ± 0.5 g) were investigated to determine the effects of total replacement of 80 g kg‐1 fish oil (diet A) with linseed oil (diet B) and soybean oil (diet C), respectively. No significant differences in weight gain rate and specific growth ratio were observed among all fish groups (p > .05). Diet A fish group had the highest, but diet B fish group had the lowest feed intake (p < .05). Feed efficiency of fish fed diet A was significantly lower than the other two fish groups (p < .05). Fish fed diet A had the lowest fillet and liver lipid contents (p < .05). Serum lactate dehydrogenase, alanine aminotransferase and aspartate aminotransferase activities of fish fed diet A were significantly lower than those of fish fed diets B and C (p < .05). The contents of linoleic acid (C18:2n6) and linolenic acid (C18:3n3) in fillets showed a significantly positive linear correlation with the diets. Serum glucose and non‐esterified fatty acid were just affected by the time point (p < .05). The lipid source, time point and interaction of both factors had significant effects on serum triglyceride, high‐density lipoprotein cholesterol and low‐density lipoprotein cholesterol (p < .05). Serum total cholesterol was only affected by interaction of time point and lipid source (p < .05), and ketone body was not affected by lipid source, time point or interaction of both factors (p > .05). In summary, total replacement of fish oil with linseed oil or soybean oil had no significant adverse effects on hybrid sturgeon growth during 84‐day period, and linoleic acid and linolenic acid in fillet were modified by dietary treatments. 相似文献
6.
A.‐K. LUNDEBYE E.‐J. LOCK D. BOYLE K. RUOHONEN M.H. BERNTSSEN 《Aquaculture Nutrition》2010,16(5):549-558
The inclusion of plant‐based ingredients in commercial fish feeds may pose a challenge because of the presence of undesirable substances, such as the pesticide endosulfan. Waterborne endosulfan is highly toxic to fish, whereas dietborne exposure has varied toxicity in different species. To investigate the systemic effects of endosulfan exposure, quadruplicate groups of Atlantic salmon (Salmo salar) were fed either 0 (control), 0.005 mg kg?1; the European Union's maximum limit, or 10 or 20 times this level (0.05 and 0.1 mg kg?1 respectively) for 95 days. There were no significant differences (P > 0.05) in liver somatic index, spleen somatic index, condition factor or growth among treatments. There were no indications of liver damage in fish from any of the groups in the biomarkers measured: plasma aspartate aminotransferase, plasma alanine aminotransferase and histopathology. Similarly, there were no apparent treatment‐related effects on the haematological parameters Hct, Hb, mean corpuscular volume, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin, and blood sodium, potassium, calcium and chloride levels were not significantly (P > 0.05) different among groups. Lipid digestibility, but not energy, protein, or glycogen digestibility, was significantly (P < 0.05) reduced at the highest exposure concentration. However, no significant differences were observed in lipid production value or lipid efficiency ratio. In contrast to previous studies, clinical histological abnormalities were not observed in the intestine, liver or spleen of endosulfan‐treated fish. 相似文献
7.
Morteza Yaghoubi Mansour Torfi Mozanzadeh Omid Safari Jasem G. Marammazi 《Fish physiology and biochemistry》2018,44(3):853-868
As amino acids (AAs) are vital molecules in the metabolism of all living organisms and are the building blocks of enzymes, a 6-week feeding trial was conducted for determining the influence of dietary essential amino acid (EAA) deficiencies on pancreatic, plasma, and hepatic enzyme activities in silvery-black porgy (initial weight 4.7?±?0.01 g) juveniles. Eleven isoproteic (ca. 47%) and isoenergetic (ca. 20.5 MJ kg?1) diets were formulated including a control diet, in which 60% of dietary nitrogen were provided by intact protein (fish meal, gelatin, and wheat meal) and 40% by crystalline AA. The other 10 diets were formulated by 40% reduction in each EAA from the control diet. At the end of the experiment, fish fed with threonine-deficient diet showed the lowest survival rate (P?<?0.05), whereas growth performance decreased in fish fed all EAA-deficient diets, although the reduction in body growth varied depending on the EAA considered. Pancreatic enzymes (trypsin, lipase, α-amylase, and carboxypeptidase A) activities significantly decreased in fish fed the EAA-deficient diets in comparison with fish fed the control diet (P?<?0.05). Fish fed with the arginine-deficient diet had the highest plasma and liver alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels (P?<?0.05). Plasma and liver lactate dehydrogenase and superoxide dismutase showed the highest and lowest values, respectively, in fish fed the arginine and lysine-deficient diets (P?<?0.05). Plasma metabolites were significantly affected by dietary EAA deficiencies (P?<?0.05). The results of this study suggesting dietary EAA deficiencies led to reduction in growth performance as well as pancreatic and liver malfunction. Furthermore, arginine and lysine are the most limited EAA for digestive enzyme activities and liver health in silvery-black porgy. 相似文献
8.
Effects of replacing fish meal with corn gluten meal on growth,feed utilization,nitrogen and phosphorus excretion and IGF‐I gene expression of juvenile Pseudobagrus ussuriensis 下载免费PDF全文
Xianyong Bu Xuqiu Lian Ying Zhang Fangyi Chen Baibing Tang Xianping Ge Yuhong Yang 《Aquaculture Research》2018,49(2):977-987
Seven isonitrogenous and isolipidic diets containing fish meal (FM) protein replaced by corn gluten meal (CGM) protein at 0% (the control, C0), 10% (C10), 20% (C20), 30% (C30), 40% (C40), 50% (C50) and 60% (C60) were fed to juvenile Pseudobagrus ussuriensis for 8‐weeks to evaluate the effects of FM protein replaced by CGM protein on growth, feed utilization, nitrogen (N) and phosphorus (P) excretion and IGF‐I gene expression of juvenile P. ussuriensis. The results showed that the replacement level up to 40% did not affect the weight gain, specific growth rate (SGR), feed intake and protein efficiency ratio, whereas these parameters were depressed by further replacement level. Apparent digestibility coefficients (ADC) of dry matter, crude protein significantly decreased, but ADC of phosphorus significantly increased with increasing dietary CGM levels (p < .05). Fish fed diets with FM protein replaced by CGM protein led to an increase in nitrogen excretion, but led to a reduction in phosphorus excretion. No significant differences were observed in alpha‐amylase and lipase activities of intestine (p > .05). The lowest pepsin activity was found in C60 group. Fish fed diet C40, C50 and C60 had significantly lower serum lysozyme activity compared with fish fed diet C0 (p < .05). The lowest plasma alkaline phosphatase activity and the highest plasma alanine aminotransferase and aspartate aminotransferase activities were observed in C60 group. Fish fed diet C60 had significantly lower hepatic IGF‐I gene expression compared with fish fed diet C10 (p < .05). Broken‐line model analysis based on SGR against the CGM substitution level indicated that the appropriate replacement level was 37.7%. 相似文献
9.
Effect of date palm (Phoenix dactylifera) seed extract as a dietary supplementation on growth performance immunological haematological biochemical parameters of common carp 下载免费PDF全文
Maryam Mohammadi Mehdi Soltani Ali Siahpoosh Seyed Pezhman Hosseini Shekarabi Mehdi Shamsaie Mehrgan Alan Lymbery 《Aquaculture Research》2018,49(8):2903-2912
This study aimed to assess the effect of date palm (Phoenix dactylifera) seed (DPS) extract at 0.5%, 1%, 2% and 4% as a dietary supplement over 60 days, on the growth and immune‐physiology of common carp (Cyprinus carpio) fingerlings. The weight gain in fish fed with 0.5% DPS was significantly greater than other treatments and fish fed a control diet with no DPS (p < 0.05). With increasing DPS dosage above 0.5%, weight gain and growth rate gradually declined. Feed conversion ratio in fish fed 0.5% DPS was lower than other treatments, but not significantly so, except for the highest DPS concentration (4%). With respect to immunophysiological variables, fish treated with 0.5% DPS leucocyte, neutrophil, lymphocyte and monocyte counts that were generally equivalent to fish on a control diet, but significantly lower than fish treated with greater concentrations of DPS (p < 0.05). Chemilumincene response and lysozyme activity, as well as levels of total protein and albumin, were higher in all treated groups than the control. The highest levels of iron and high density lipoprotein (HDL) were observed in fish fed with 0.5% DPS, while the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), glucose, cholesterol, triglyceride, low density lipoprotein (LDH), thyrotropin, thyroxine and triiodothyronine were lower level in fish fed with 0.5% DPS than in all other treatments. In aquaculture practice, use of 0.5% DPS in commercial carp feed can improve the growth rate and condition of fish. 相似文献
10.
Effect of dietary GroBiotic®‐A supplementation as a prebiotic on the intestinal microflora,growth performance,haemato‐serological parameters,survival rate and body composition in juvenile beluga (Huso huso Linnaeus, 1754) 下载免费PDF全文
M. Adel R. Safari S. Yeganeh M. Binaii M. Ghiasi S. Ahmadvand 《Aquaculture Nutrition》2017,23(3):492-499
The objectives of the present study were to investigate of GroBiotic®‐A (GBA) on growth, autochthonous intestinal microbiota and haemato‐serological parameters of beluga juvenile. A total of 180 fish (40.82 ± 5.81 g) were fed diets containing graded levels of GBA (0, 5, 10 and 20 g kg?1 diet) for 8 weeks. No significantly differences in body composition, total viable aerobic bacteria, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, neutrophils, eosinophils, monocytes, albumin, glucose, triglyceride, cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase were detected between treatment groups. The fish fed on 10 and 20 g kg?1 GBA significantly showed higher lactic acid bacteria, final weight, body weight increases, weight gain and lower feed conversion ratio compared with the control and 5 g kg?1 groups. The group fed 20 g kg?1 GBA showed a highly significant difference in condition factor, survival rate, final length, total red and white blood cells, lymphocytes, haematocrit, haemoglobin, total protein, total immunoglobulin and lysozyme activity. The specific growth rate of the treatment groups was significantly elevated compared with the control groups. These results indicated that GBA at level 20 g kg?1 improved growth, welfare and survival of beluga juvenile. 相似文献
11.
This study was conducted to evaluate the effect of ethanolic extract of propolis (EEP) on growth performance and plasma biochemical
parameters of rainbow trout (Oncorhynchus mykiss). Graded levels of EEP [0 (control), 1, 2, and 4 g kg−1 diet] were fed to trout juveniles (mean weight 7.73 ± 0.17 g) for 10 weeks. Dietary EEP supplementation regardless of inclusion
level significantly improved the specific growth rate of fish. Similarly, supplemental EEP generally improved the feed efficiency
ratio and protein efficiency ratio, but no significant differences were observed between the 1 g kg−1 EEP group and the control group. In addition, dietary EEP supplementation generally increased the plasma superoxide dismutase,
lysozyme, total antioxidant capacity, glutathione peroxidase, and catalase activities, but decreased the plasma malondialdehyde
level. The plasma triglycerides level was significantly lower in the 1 or 4 g kg−1 EEP group as compared with the control group. Dietary EEP supplementation generally decreased the plasma aspartate aminotransferase
and alanine aminotransferase activities, but increased the hepatic aspartate aminotransferase and alanine aminotransferase
activities. These results indicate the potential to use the EEP as a growth promoter, hepatoprotective agent, and immunostimulant
for rainbow trout. 相似文献
12.
Jos María García Beltrn Cristbal Espinosa Francisco A. Guardiola Simona Manuguerra Andrea Santulli Concetta María Messina María ngeles Esteban 《Aquaculture Research》2019,50(11):3181-3191
Dehydrated lemon peel (DLP) at two levels (1.5% and 3%) was included for 30 days in the diet of gilthead seabream (Sparus aurata L.) and after 15 and 30 days the serum and skin mucus of fish were analysed. In serum, both experimental diets led to an initial decrease in glucose and lactate after which the levels became comparable to the control. Dietary DLP modulated the activity of transaminases, alanine aminotransferase and aspartate aminotransferase. The total antioxidant status showed a progressive increase in relation to levels of inclusion of DLP and time of administration (p < .05). In skin mucus, some biomolecular markers related to general stress, oxidative stress and apoptosis exhibited variations that suggest an adaptive response of fish to the new metabolic situation, resulting from the experimental diet. The inclusion of DLP in the diet seems to positively modulate the central metabolism and welfare of gilthead seabream. 相似文献
13.
In order to investigate the effect of anesthesia on serum parameters, Beluga, Huso huso (L.) were blood-sampled immediately without anesthesia (control) or subjected to following anesthesia procedure: 40, 120,
and 240 s exposure to 3,000, 700, and 500 mg l−1 clove solution, respectively. Blood samples were collected after these periods, when fish were immobile and reached stage
4 anesthesia. Results showed that cortisol and glucose levels were significantly high in 700 and 500 but not 3,000 mg l−1 group compared to control. Serum lactate levels were significantly high in 500 mg l−1 group compared to control group. Lactate levels were not significantly differed between control, 3,000, and 700 mg l−1 groups. There were no significant differences in serum levels of cholesterol, total protein, lactate dehydrogenase, aspartate
aminotransferase, alanine aminotransferase, Na+, Cl−, K+, and Ca2+. Results suggest that rapid anesthesia with higher dose is better than slow anesthesia with lower dose for blood sampling
in Beluga. 相似文献
14.
Ahmed Mokrani Hualiang Liang Ke Ji Hopeson Chisomo Kasiya Qiang Yang Xianping Ge Mingchun Ren Xuezhi Zhu Liangkun Pan Ajun Sun 《Aquaculture Research》2019,50(2):557-565
Six diets were formulated to investigate the success of fish meal (FM) replacement by plant proteins; diet 1 reflected a commercial feed (8% FM), diet 3 contained 4% FM, and diet 5 was devoid of FM. Whereas, diet 2, diet 4, and diet 6 reflected diet 1, diet 3, diet 5, respectively, and supplemented with essential amino acid (EAA). At the end of 8‐week trial, there was no significant difference in survival rate. Significantly higher final weight, weight gain rate, and specific growth rate were recorded in the group fed diet 2 compared with the other treatments (except diet 4) (p < 0.05). Feed conversion ratio of fish fed diet 2 was significantly lower than those fed diets 1, 3, and 5 (p < 0.05). The lowest feed intake and highest protein efficiency rate were found in fish fed diet 2 (p < 0.05). There was no significant difference in whole body compositions between treatments. Plasma aspartate transaminases, alanine aminotransferase, and glucose were significantly affected by dietary treatments (p < 0.05), while plasma protein and albumin contents were not influenced by the treatments. The relative expression of target of rapamycin (TOR) and phosphatidylinositol 3‐kinase, regulatory subunit 1 (alpha) (PIK3R1) in fish fed diet 3 (4% FM) were significantly down‐regulated compared with those fed diet 6 for TOR and diets 4 and 6 for PIK3R1 (p < 0.05). Insulin receptor substrate 1 (IRS‐1) and janus kinase 3 (JAK3) expressions were fluctuated, with the higher levels in fish fed diets 4 and 6. In conclusion, the findings of this study indicate that plant protein mixture supplemented with EAA could be used to substitute FM in practical diet for Megalobrama amblycephala. 相似文献
15.
Shunmei Liu Xiaonan Zang Bin Liu Xuecheng Zhang Arunakumara K K I U Xiaoqing Zhang & Bing Liang 《Aquaculture Research》2007,38(12):1283-1292
The present study investigated the effect of supplementing feed with transgenic Synechocystis sp. PCC6803 containing the Paralichthys olivaceus growth hormone (GH) gene on growth, feed intake and feed efficiency ratio, muscle composition, haematology and histology of turbot (Scophthalmus maximus L.). At the end of the 40‐day feeding trial, the specific growth rate of fish fed the supplemented feed with 1.0% transgenic Synechocystis sp. PCC6803 was 21.67% higher (P<0.05) than that of control fish. Although body weight and feed efficiency ratio significantly increased (P<0.05) in fish fed the diet supplemented with transgenic alga, feed intake and condition factor of the experimental fish were unaffected. Muscle composition analysis showed that the protein content was positively influenced by the transgenic alga, whereas the lipid content was unaffected. Haematological parameters, including red blood cell, white blood cell, haemoglobin, and serum biochemical indices, such as enzyme activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, concentrations of total protein, glucose, blood urea nitrogen, creatinine, triglyceride and cholesterol and ion levels of K, Na, Cl, P were not influenced by supplementing the transgenic Synechocystis sp. PCC6803. Furthermore, no histopathological alterations were induced by transgenic alga treatment in the stomach, intestine, liver, spleen and kidney of the experimental fish. The results of the present study indicated that transgenic Synechocystis sp. PCC6803 containing P. olivaceus GH gene is an efficient growth promoter and a safe feed additive for fish. 相似文献
16.
Effect of dietary eucalyptol on stress markers,enzyme activities and immune indicators in serum and haematological characteristics of common carp (Cyprinus carpio) exposed to toxic concentration of ambient copper 下载免费PDF全文
Seyyed Morteza Hoseini Seyed Hossein Hoseinifar Hien Van Doan 《Aquaculture Research》2018,49(9):3045-3054
In the present study, protective effects of dietary eucalyptol were investigated against copper toxicity in common carp (Cyprinus carpio). The fish were fed with diets supplemented with 0 (control), 0.5% and 1% eucalyptol for 14 days before exposure to 0.25 mg/L ambient copper for 7 days. Blood samples were taken from the fish before and 1, 3 and 7 days after copper challenge. Before copper challenge, eucalyptol significantly decrease serum aspartate aminotransferase (AST) activity, but increase blood WBC, RBC, hematocrit and haemoglobin, serum lysozyme, total immunoglobulin (Ig) compared with the control. Copper exposure led to stress, tissue damage and immunosuppression. After the copper challenge, significant elevation in serum cortisol, glucose, alanine aminotransferase (ALT) and AST, but decrease in blood RBC and WBC, and serum lysozyme, complement (ACH50) and total immunoglobulin (Ig) was observed. Eucalyptol administration significantly mitigated the increase in serum cortisol, glucose, ALT and AST, and decrease in WBC, ACH50, lysozyme and total Ig. Dietary 1% eucalyptol had significantly higher protective effects than 0.5% in the case of serum AST, lysozyme, total Ig and ACH50. In conclusion, eucalyptol has beneficial health effects on common carp and enables to mitigate copper‐induced stress, tissue damage, and immunosuppression. Eucalyptol at the dietary 1% level is recommended to protect common carp against adverse effects of copper toxicity. 相似文献
17.
Zahra Samavat Mehdi Shamsaie Mehrgan Shahla Jamili Mehdi Soltani Seyed Pezhman Hosseini Shekarabi 《Aquaculture Research》2019,50(9):2496-2504
In this study, total phenolic and flavonoid contents of grapefruit peel extract (GPE) were equal to 117.3 ± 0.3 µg of gallic acid/mg and 39.30 ± 0.1 µg of quercetin/mg respectively. Caspian white fish (n = 180, 4 ± 0.9 g body weight) was fed with supplemented diets, including 0, 6.25, 12.5 and 25 mg of GPE/kg for 60 days at 25 ± 1°C. The growth performance was markedly improved in fish fed with 25 mg/kg of GPE compared to others (p < 0.05). Moreover, fish fed with 25 mg/kg of GPE showed a significant increase in red blood cell (2.65·106 cell/mm3), white blood cell (17.75·103 cell/mm3), packed cell volume (48%) and haemoglobin concentrations (8.75 g/dl) compared to the control (p < 0.05). However, the highest alanine aminotransferase (140 U/L), alanine transaminase (14.5 U/L), and alkaline phosphatase (18.5 U/L) were observed in control group. Morphological analysis of intestine revealed the highest amount of villus width (8.4 µM), height (32.86 µM) and surface area (342.7 µM2) in fish fed with 25 mg/kg of GPE (p < 0.05). In conclusion, supplementing feed with GPE at 25 g/kg can improve growth performance and haemato‐biochemical parameters of Caspian white fish fry. 相似文献
18.
Healthy crucian carp (Carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 μg MC-LR equiv kg−1 BW. High mortality (100%) was observed within 60 h post injection in the high-dose group. In the treated fish, activities
of four plasma enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and
lactate dehydrogenase (LDH), all showed substantial increases, with both dose and time-dependent effects. These increases
of enzyme activity indicate severe impairment occurred in the liver of crucian carp over time. Plasma concentrations of energy-related
biomolecules including glucose (GLU), cholesterol (CHO), triglyceride (TG), and total protein (TP) showed marked changes in
the high-dose group, possibly a nutritional imbalance correlated with the liver injury caused by intraperitoneal exposure
to crude cyanobacterial extracts. 相似文献
19.
The use of fenugreek seed meal in fish diets: growth performance,haematological and biochemical parameters,survival and stress resistance of common carp (Cyprinus carpio L.) 下载免费PDF全文
Zahra Roohi Mohammad Reza Imanpoor Valiolah Jafari Vahid Taghizadeh 《Aquaculture Research》2017,48(3):1209-1215
The present study was conducted to investigate the effects of fenugreek seed meal (FKSM) on growth performance, blood haematological and biochemical factors, survival and stress resistance of the common carp (2.46 ± 0.06 g) fingerlings. Four practical diets containing 0 (control), 0.5%, 1% and 1.5% FKSM were used to feed fish. Fish were fed on the tested diets at a rate of 3% of body weight for 8 weeks. Results indicated that fish fed the control diet had a significantly lower weight gain, specific growth rate and condition factor compared to the other treatments (P < 0.05). Feed conversion ratio decreased significantly in fish fed diets containing FKSM. There were no significant differences in glucose levels, red blood cell count, haematocrit and haemoglobin between treatment groups and the control group (P > 0.05). Significantly lower white blood cells count was observed in fish treatment with FKSM when compared with the control group (P < 0.05). The cholesterol level in group fed supplemented diet by 1% fenugreek significantly lower than other groups (P < 0.05). Total protein levels were significantly higher in all treatments compared to the control (P < 0.05). No mortality was recorded during the feeding trial. In salinity stress experiment, highest survival rate belong to fish fed supplemented diet by 1% fenugreek. These results indicate that FKSM can be considered as a beneficial dietary supplement for improving the growth performance and blood indices of common carp fingerling. 相似文献
20.
Silymarin, an extract from “milk thistle” (Silybum marianum) plant is traditionally used as herbal medicine. The present study was conducted to investigate the clinical effects and
possible side effects of silymarin on biochemical blood parameters of rainbow trout (Oncorhynchus mykiss). Fishes were treated with 0 (control), 100, 400, and 800 mg of silymarin per kg of food during 4 weeks. Plasma alanine aminotransferase
(ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), creatine kinase (CK), glucose,
total protein, creatinine, triglyceride, cholesterol, urea, uric acid and liver cellular total antioxidant, and protein content
were measured after 7, 14, and 28 days of silymarin treatment. The results showed that oral administration of silymarin in
fish significantly reduced plasma glucose and cholesterol levels and relatively increased plasma total protein and globulin
concentrations (P < 0.05). Increasing plasma albumin levels indicate the important role of albumin in drug transportation in circulatory system
of fish. Silymarin also stabilized cellular membrane structure and regulated the levels of AST, ALT, ALP, CK, and LDH activity.
In conclusion, on the basis of these results, oral administration of silymarin up to 400 mg per 1 kg of food has no side effect
on blood biochemical and clinical parameters of fishes. However, oral administration of 800 mg/kg- of silymarin caused cytotoxicity
and modifications in blood biochemical parameters of fish. 相似文献