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1.
Agnès Flore Ndomo Léonard Tinkeu Ngamo Léon Azéfack Tapondjou Félicité Mbiapo Tchouanguep Thierry Hance 《Journal of pest science》2008,81(4):227-234
The bioefficacies of essential oil and aromatized clay powder based on the mixture of clay and essential oil extracted from
Clausena anisata were evaluated for their insecticidal activities and their effects on progeny production of Acanthoscelides obtectus. Contact toxicity assayed by coating on bean grains showed that these chemicals caused significant mortality of the test insect.
Beetle mortality was dose dependant and after 2-day exposure the aromatized clay powder was more toxic (LD50 = 0.069 μl/g grain) than the pure essential oil (LD50 = 0.081 μl/g grain). There was, however, a highly significant loss of toxicity after 24 and 36 h following treatment with
essential oil and aromatized powder, respectively. Both aromatized clay powder and pure essential oil considerably reduced
the F1 progeny insect production. The essential oil evoked moderated repellent action and high fumigant toxicity (LC50 = 0.093 μl/cm3) against adults of A. obtectus. These results suggest that the clay powder could be used to stabilize the essential oil to increase its efficacy for use
as an alternative to synthetic insecticides. 相似文献
2.
Akram Taghizadeh Saroukolai Saeid Moharramipour Mohammad Hadi Meshkatalsadat 《Journal of pest science》2010,83(1):3-8
Red flour beetle Tribolium castaneum (Herbst) and rice weevil Sitophilus oryzae (L.) are considered to be the major insect pests in storage. Essential oils from aromatic plants are recognized as proper
alternatives to fumigants. Thymus persicus (Ronniger ex Rech. f.) is one of these plants that have medicinal properties and is indigenous to Iran. The essential oil
was obtained from aerial parts of the plant and analyzed by GC and GC–MS. Carvacrol (44.69%) and thymol (11.05%) were the
major constituents of the oil extracted. In this experiment, fumigant toxicity of the essential oil was studied against T. castaneum, S. oryzae at 27 ± 1°C and 60 ± 5% RH in dark condition. The adult insects were exposed to the concentrations of 51.9, 111.1, 207.4
and 370.4 μl/l air to estimate median lethal time (LT50) values. The fumigant toxicity was increased in response to increased essential oil concentrations. The LT50 values at the lowest and the highest concentrations tested were ranged from 28.09 to 13.47 h for T. castaneum, and 3.86 to 2.30 h for S. oryzae. It was found that S. oryzae adults were much more susceptible to the oil than T. castaneum. After 24 h of exposure, the LC50 values (95% fiducial limit) for T. castaneum and S. oryzae were estimated to be 236.9 (186.27–292.81) and 3.34 (2.62–4.28) μl/l air, respectively. These results suggest that T. persicus essential oil merits further study as potential fumigant for the management of these stored-product insects. 相似文献
3.
M. S. Islam M. Mahbub Hasan W. Xiong S. C. Zhang C. L. Lei 《Journal of pest science》2009,82(2):171-177
The biological activity of essential oil extracted from coriander, Coriandrum sativum L. (Apiaceae) against eggs, larvae and adults of Tribolium castaneum (Herbst) was investigated in a series of laboratory experiments. Bioassays conducted in air-tight glass chambers showed vapour
toxicity and strong repellency on filter paper arena test towards all the stages used. Responses varied significantly (P < 0.001) within insect stages, dosages and exposures. At 12.0 μg/ml dosage, mortality of adults reached 95%, but for 12-,
14- and 16-days larvae, mortalities were 65, 74 and 82%, respectively. On the developmental inhibition, individuals fumigated
at the larval stage confirmed that the percentage of larvae reaching to pupal stage and pupae to adult stage, decreased significantly
(P < 0.001) with increasing dosage concentration. The oil fumigation yielded 100% mortality for T. castaneum larvae, pupae and adults at 0.08 μg/ml dosage. The oil had fumigant activity against eggs and the toxicity progressively
increased with increased exposure times and concentrations. One hundred per cent T. castaneum egg mortalities were obtained with oil fumigation at 20 μg/ml and 96 h exposure period. C. sativum oil also showed highly repellent activity to the adults of T. castaneum, with overall repellency was 90% and at a dosage of 12 μg/ml repellency was 100% in a filter-paper arena test. 相似文献
4.
Fumigant activity of essential oil vapors distilled from Carum copticum C. B. Clarke and Vitex pseudo-negundo Hand I. MZT. was tested against eggs, larvae and adults of Callosobruchus maculatus (F.). Fumigant toxicity was assessed at 27 ± 1°C and 60 ± 5% RH, in dark condition. The influence of different concentrations of
the essential oil vapors on egg hatchability, larval and adult mortality was significant. Data probit analysis showed that
lethal concentration of the essential oil to kill 50% of the population (LC50) for egg, larvae and adult were found to be 1.01, 2.50 and 0.90 μl/l air of C.
copticum oil, followed by 2.20, 8.42 and 9.39 μl/l air essential oil of V. pseudo-negundo, respectively. Between these essential oils, C. copticum was almost more toxic than V. pseudo-negundo on all growth stages of C. maculatus. The present study suggests that essential oils from these medicinal plants may be potential grain protectants as botanical
alternative fumigants and could be used in the management of various life stages of C. maculatus. 相似文献
5.
Toxicity and repellency effects of several pure compounds (of plant origin) against the confused flour beetle (Tribolium confusum) were investigated in a filter paper test. The compounds were α-pinen, β-pinen, 4-allyl-anisol, camphor, 1,8-cineol, eugenol,
linalool, menthol, piperin, terpineol, thymol and zimtaldehyde. Five compounds (4-allyl-anisol, linalood, terpineol, thymol
and zimtaldehyde) caused enough mortality of this insect to allow the determination of LD50 and LD95 values. Zimtaldehyde and 4-allyl-anisol showed the strongest toxicity effects with LD50 values of 0.04–0.05 μl/cm2 within an exposure period of 24 h. When the isolated fumigant effects were tested by separating the insects from the filter
paper, the toxicity of zimtaldehyde (LD50=0.29 μl/cm2) was much lower than that of 4-allyl-anisol (LD50=0.10μl/cm2). 相似文献
6.
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• We studied the effects of acorn cotyledon loss by insect damage and artificial excision on germination and seedling survival
of Quercus variabilis, to determine whether insect infestation decreases acorn germination and seedling establishment; and to what extent cotyledon
damage can affect acorn germination and seedling establishment. 相似文献
7.
Barbara Conti Angelo Canale Pier Luigi Cioni Guido Flamini Alessandro Rifici 《Journal of pest science》2011,84(2):219-228
Essential oils from Hyptis suaveolens (L.) Poiteau (Lamiaceae) and Hyptis spicigera Lamarck (Lamiaceae) were first analysed by gas chromatography and by gas chromatography/electron impact mass spectroscopy
and then evaluated for toxicity and repellent activity against Sitophilus granarius (L.) (Coleoptera: Dryophthoridae), one of the most serious worldwide stored grain pests. Fifty-six compounds have been identified
in the essential oil of H. suaveolens: monoterpene hydrocarbons were the most represented volatiles (64.1%), followed by sesquiterpene hydrocarbons (24.0%), oxygenated
monoterpenes (8.1%) and oxygenated sesquiterpenes (2.4%). Diterpenes and non-terpene derivatives were scarcely represented.
Sixty compounds have been identified in the essential oil of H. spicigera: monoterpene hydrocarbons were the most represented class of volatiles (70.4%), followed by sesquiterpene hydrocarbons (22.6%).
Results from topical applications on insects showed that both essential oils had an effective insecticidal activity. The complete
kill of S. granarius was observed 24 h after treatment at a minimum effective dose of 0.4 and 0.6 μl per insect with H. suaveolens and H. spicigera oil, respectively. Results from repellency tests showed that the two essential oils had a repellent activity on S. granarius adults: at the lowest dose (2 × 10−4 μl oil per cm2), H. spicigera essential oil exhibited a higher repellent effect in comparison to H. suaveolens. No significant differences were observed for the repellent effect between the two essential oils at the highest dose (2 × 10−2 μl oil per cm2). 相似文献
8.
Shi-Guang Li Mao-Ye Li Yan-Zhang Huang Ri-Mao Hua Hua-Feng Lin Yu-Jie He Lin-Lin Wei Zhu-Qin Liu 《Journal of pest science》2013,86(4):677-683
The insecticidal activity of Illicium verum Hook. f. against Sitophilus zeamais Motschulsky adults were identified, and the underlying mechanisms were studied. Extracts from I. verum fruits in methyl alcohol (MA), ethyl acetate (EA), and petroleum ether (PE) were tested by fumigation in a hermetic container to determine their toxicity. The effects of the three extracts on the activity of acetylcholinesterase (AChE) and glutathione S-transferases (GSTs) of S. zeamais were determined in vivo. All extracts showed strong fumigant activity. The fumigant effects were enhanced with increased dosage and prolonged exposure time. 1.25, 2.50, 5.00, 10.00, and 20.0 mg/l doses of the MA, EA, and PE extracts caused the mortalities from 8.37 to 90.26 %, 21.81 to 95.89 %, and 15.84 to 92.57 %, respectively, at 72 h after treatment. Consequently, the most effective dose of the MA, EA, and PE extracts is the 20.0 mg/l. The LD50 of the MA, EA, and PE extracts at 72 h after treatment were 7.10, 3.93, and 4.55 mg/l, respectively. The activities of AChE and GSTs were notably inhibited by the three extracts, as compared with the control, with strong dose- and time-dependent effects. The inhibition strength of the three extracts on AChE and GSTs activities were in the following order: EA extract > PE extract ≥ MA extract. Therefore, I. verum extracts could be explored as novel natural fumigants for the future control of stored-product insect pests. 相似文献
9.
The efficacy of NeemAzal powder, two local neem (Azadirachta indica
) products [neem seed powder (NSP) and neem seed oil (NSO)], as well as a local Lamiaceae, Plectranthus glandulosus leaf powder, applied at four different rates for the control of Sitophilus zeamais was determined. Mortality was recorded 1, 3, 7 and 14 days after S. zeamais infestation, followed by the determination of F1 progeny production. Grain damage, population increase and grain germination were assessed for treated grains that were stored
for 4 months. Grains treated with P. glandulosus powder and NSP had relatively low mortality (5.0–22.5%) after 3 days whereas NeemAzal and NSO had higher mortality (55.0–98.8%).
Maximum mortality of 99, 100, 96 and 74% were achieved for NeemAzal (12 g/kg after 14 days), NSO (4 ml/kg after 7 days), NSP
(40 g/kg after 14 days) and P. glandulosus powder (40 g/kg, after 14 days), respectively. In the same order, 7-day LC50 values were 0.02 g/kg, 1.46 ml/kg, 12.44 g/kg and 28.9 g/kg. The three neem products greatly reduced progeny emergence, while
P. glandulosus powder was less effective. NeemAzal protected the grains against S. zeamais damage better than the local neem products, which in turn provided far better grain protection against the weevil damage
than P. glandulosus powder. NeemAzal was superior to the local neem products in grain protection against germination loss, with P. glandulosus being the least effective. NeemAzal and NSO had sufficient efficacy to be a component of an integrated management package
for S. zeamais. 相似文献
10.
Bioassays were performed to determine the antifungal and insecticidal activity of clove essential oil (EO), several botanical compounds (eugenol, carvacrol, allylisothiocyanate (AITC) and ethyl formate (EtF)) against mycotoxigenic fungi (Aspergillus westerdijkiae and Fusarium graminearum) and the rice weevil, Sitophilus oryzae. Antifungal activity was quantified by measuring conidia germination inhibition and mycelial growth inhibition zone to determine the minimum inhibitory concentration (MIC). Mortality of insects was determined through either contact toxicity assay (impregnated filter paper) or fumigation toxicity assay (airtight exposure chamber). A four-parameter logistic regression of fungi inhibitory growth rate to the dose of tested substances, either by the agar diffusion assay or the micro-atmosphere test, showed that AITC had antifungal and sporicide activity. The concentration at MIC for A. westerdijkiae and F. graminearum was 24.2 and 19.8???l?l?1, respectively, whereas clove essential oil (EO) was 755 and 352???l?l?1 after 72?h incubation period. Bioassays with S. oryzae showed that clove EO contact insecticidal activity was similar to pure eugenol and carvacrol: LD90s were 366, 385 and 442???l?dm?2, respectively. The fumigation insecticidal activity of AITC was LC95?=?10.8???l?l?1. For EtF and clove EO, only the LC50 could be accurately determined after 24?h exposure time which was observed at 41 and 210???l?l?1, respectively (vs. 6.4???l?l?1 for AITC LC50). The combined antifungal activity on mycotoxigenic seed-borne fungi and insecticidal activity against the rice weevil demonstrated that AITC in vapour phase may be a promising active substance for the preservation grain stored in unsafe conditions with a risk of fungal growth. 相似文献
11.
Crataeva magna (Lour.) DC (synonym C. nurvala) is a high-value Indian medicinal tree. The multiple uses of C. magna have resulted in its over-exploitation. Erratic seed germination combined with destructive harvesting and habitat destruction
in the form of deforestation has added to the enormity of the problem. Therefore, the need for conservation of this plant
is vital. Development of an efficient micropropagation protocol will play a significant role in meeting the requirement of
quality planting material for commercial cultivation thereby conserving the species in its natural habitat. In the present
study, shoot multiplication was achieved by culturing single node segments derived from a field grown tree on Murashige and
Skoog’s (MS) medium supplemented with 2.66 μM N6-benzyladenine, 1.39 μM Kinetin (Kn), 0.57 μM indole-3-acetic acid (IAA),
3% sucrose and 0.2% gelrite. 96% rooting was achieved within 22 days by culturing the in vitro formed shoots on half strength
MS medium with 11.42 μM IAA, 9.8 μM indole-3-butyric acid, 0.46 μM Kn and 198.25 μM phloroglucinol. Following a simple hardening
procedure involving sequential transfer of plants to a greenhouse, polyhouse, and shade net, the tissue-cultured plants were
transferred to the field where the survival rate was 100%. To this date 500 plants have been produced. Inter simple sequence
repeat analysis has confirmed genetic uniformity of the tissue-cultured plants. 相似文献
12.
The insecticidal activities of the extracts, fractions, and pure compound of Sterculia foetida (L.) seeds were investigated against three major stored product pests, namely, Sitophilus oryzae (L.), Callosobruchus chinensis (L.), and Tribolium castaneum (H.), through contact and fumigation bioassay. Responses varied with test chemicals, insect species, exposure times, and
doses individually. The biologically active constituent of the S. foetida seeds was characterized as a cyclopropene fatty acid structurally elucidated as (2n-octylcycloprop-1-enyl)-octanoic acid (I) by spectroscopic analysis. The filter paper application of, (2n-octylcycloprop-1-enyl)-octanoic acid (I) at 0.2 mg/cm2 caused 100% mortality to all test insects 2 days after treatment (2 DAT). Among the chromatographic fractions, only the pure
hexane-eluted fraction caused 100% mortality at 0.3 mg/cm2 to S. oryzae and C. chinensis 2 DAT, while the dose required to obtain the same percentage of mortality with the crude seed extract was 0.6 mg/cm2. Under all treatment conditions, T. castaneum exhibited less susceptibility, taking longer than the other insects to show symptoms of toxicity. In terms of fumigant activity,
the compounds appeared to be more effective in a vapor form against all the three insects in closed containers compared with
open containers. These results suggest that the insecticidal activities of the test compounds were attributable to contact
mode of action, although there was also significant fumigant toxicity. The results indicate that the bioactive compound isolated
from S. foetida could act as a potent insecticide against S. oryzae, C. chinensis, and T. castaneum populations. 相似文献
13.
Domenico Rongai Claudio Cerato Luca Lazzeri Sandro Palmieri Giampiero Patalano 《Journal of pest science》2008,81(4):179-185
During laboratory tests, mineral oil in water emulsion (2%), containing Brassicaceae seed meals (2 g l−1), induced total mortality (100%) among adult female California red scales on detached orange fruits, whereas only 47.5% of
insect mortality was observed with the same mineral oil when used alone. These results were partially confirmed by two preliminary
field trials. With the aim of improving biodegradability and the environmental impact of the insecticidal formulation, mineral
oil was replaced by a vegetable oil without observing any significant variation of the product’s efficacy. In addition, a
clear “dose-effect” of meal in mineral and vegetable oil was also demonstrated. The formulation, essentially based on oil,
meals and some minor additives, shows the following advantages: (1) high insecticidal activity against an insect pest difficult
to control such as Aonidiella aurantii (Maskell); (2) simultaneous control of several pathogens seems to be possible; (3) non-appearance of resistance phenomena
in plant pathogens; and finally, (5) absence of phytotoxicity. 相似文献
14.
Dusanee Thanaboripat Kowit Nontabenjawan Kwanruen Leesin Detchpol Teerapiannont Oratai Sukcharoen Rararat Ruangrattanamatee 《林业研究》1997,8(1):39-42
The inhibitory effect of crude extracts of garlic, clove and carrot at concentrations of 20,000, 40,000, 60,000, 80,000 and
100,000 μg/mL on growth ofAspergillus flavus and aflatoxin production in rice was investigated. The results showed that garlic, clove and carrot could inhibit the growth
ofA. flavus and aflatoxin production. Garlic and clove at 100,000 μg/mL highly reduced the level of aflatoxin from 5.94 to 0.15 and 0.06
μg/g respectively whereas carrot at 20,000 μg/mL reduced the most level of aflatoxin from 5.94 to 0.03 μg/g. Garlic, clove
and carrot at 100,000μg/mL also inhibited the mould growth. The most effective herb for inhibiting the growth ofA. flavus and aflatoxin production was garlic.
Responsible Editor: Sun Yueqi 相似文献
15.
A method for efficient in vitro regeneration of Leucaena leucocephala cv. K636 was developed using immature zygotic embryos as the explant. Multiple shoot induction was achieved by culturing
the explants on half-strength Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) (0.03 to 1.5 μM). The maximum
number of shoots per explants was achieved in 0.26 μM TDZ-supplemented media. TDZ concentration significantly influenced the
induction of multiple shoots as well as the shoot-length. TDZ at 0.35 μM or higher concentrations resulted in abnormal stunted
shoots. Full-strength MS media devoid of any plant growth regulator were used for shoot elongation. In vitro root induction
was achieved in half-strength MS media supplemented either with 0.54 μM 1-naphthaleneacetic acid (NAA) or with 14.76 μM indole-3-butyric
acid (IBA) in combination with 0.23 μM kinetin (Kn). Media supplemented with 14.76 μM IBA in combination with 0.23 μM Kn induced
twofold–threefold more roots than media supplemented with 0.54 μM NAA. However, the average root length was lower in 14.76 μM
IBA in combination with 0.23 μM Kn than in 0.54 μM NAA. Regenerated plants were maintained under normal condition after hardening.
Plants, which were rooted on media supplemented with 14.76 μM IBA in combination with 0.23 μM Kn showed higher survival rate
during hardening than those rooted on 0.54 μM NAA supplemented media. 相似文献
16.
Ismail Amri Samia Gargouri Lamia Hamrouni Mohsen Hanana Tarek Fezzani Bassem Jamoussi 《Journal of pest science》2012,85(2):199-207
The chemical composition of essential oils isolated by hydrodistillation from the aerial parts of Tunisian Pinus pinea Linn. was analyzed by GC and GC/MS. Sixty-six compounds were identified, representing 98.5% of total oil, which was found
to be rich in monoterpene hydrocarbons (73.1%) particularly limonene (54.1%), α-pinene (7.7%), and β-pinene (3.4%). The yield
and the physico-chemical properties were determined. Results of the antifungal activity study by in vitro contact assay showed
that P. pinea oil significantly inhibited the growth of ten plant pathogenic fungi. Moreover, herbicidal properties of the oil, tested
on Sinapis arvensis L., Lolium rigidum Gaud., and Raphanus raphanistrum L., indicated that the oil completely inhibited the seed germination at a high concentration, while at low doses the oil
acted by decreasing and delaying the germination and inhibiting the seedling growth of all tested weeds unlike the commercial
herbicide. Our results showed that P. pinea essential oil could be valorized as bioproduct for biocontrol of weeds and fungal plant diseases. 相似文献
17.
A micropropagation method for Jaal (Salvadora persica)—a tree of arid horticulture and forestry has been developed. Nodal segments of fresh shoot sprouts originated from axillary
buds obtained from a plant around 35–40 years old lopped plant were used as explants for establishment of in vitro cultures.
Surface-sterilized explants produced optimum number of shoots through activation of axillary buds on Murashige and Skoog’s
(MS) medium containing 8.88 μM BA (6-benzyladenine) + additives (25 mgl−1 each of adenine sulphate, arginine, citric acid, 50 mgl−1 ascorbic acid). The shoot multiplication was influenced by the successive transfer of the mother explants for 4–5 passages.
The maximum number (23.1 ± 0.73 shoots per explant) of shoots were regenerated on MS supplemented with 1.11 μM BA + 1.16 μM
Kn (Kinetin) + 0.54 μM NAA (α-naphthalene acetic acid). About 90% shoots pulse-treated with a combination of 2460.27 μM Indole-3-butyric
acid (IBA) + 494.56 μM NOA (2-naphthoxy acetic acid) were rooted ex vitro on soilrite within 15–18 days. Over 80% cloned plantlets
were hardened successfully in a green house and transferred to polybag/pots. 相似文献
18.
Nitai Debnath Sumistha Das Dipankar Seth Ramesh Chandra Somesh Ch. Bhattacharya Arunava Goswami 《Journal of pest science》2011,84(1):99-105
Fumigants and residual insecticides are commonly used to combat stored grain pests. In recent years, consumer awareness of
the health hazard from residual toxicity and the growing problem of insect resistance to these conventional insecticides have
led the researchers to look for alternative strategies for stored grains protection. For example, diatomaceous earth (DE)
can be effective against stored grain insects. In this study, DE was used to design amorphous nano sized hydrophilic, hydrophobic,
and lipophilic silica in 15–30 nm size range. Nanocides are expected to reduce the volume of application and kinetics of development
of resistance in pests. We hypothesized that surface-functionalized silica nanoparticle (SNP) might be a viable alternative
to conventional pesticides. Entomotoxicity of SNP was tested against rice weevil Sitophilus oryzae and its efficacy was compared with bulk-sized silica (individual particles larger than 1 μm). Amorphous SNP was found to
be highly effective against this insect pest causing more than 90% mortality, indicating the effectiveness of SNP to control
insect pests. 相似文献
19.
Powders and essential oils were prepared from the aerial parts of Cinnamomum camphora, Ocimum basilicum, Chenopodium ambrosioides, and seeds of Pimpinella anisum. Their adulticidal activities and effects on the F1 progeny of Trogoderma granarium (Everts) and Tribolium castaneum (Herbst) were evaluated. The chemical composition of the plant oils were identified by gas chromatography (GC) and GC/mass
spectrometry (MS). All of the tested botanicals showed insecticidal activities against the test insects in a dose-dependent
manner with T. granarium was more susceptible to the tested plant products than T. castaneum. At a concentration of 5 g kg−1, many of the plant powders caused 100% mortality of both insects after 14 days of exposure. The powders of C. camphora, O. basilicum, and C. ambrosioides were effective against T. granarium, while that of C. ambrosioides caused 100% adult mortality of T. castaneum under the same assays conditions. A dose of 1.50 ml cm−2 of the oils of C. camphora and O. basilicum completely controlled T. granarium, while 100% mortality of T. castaneum adults was recorded with P. anisum oil. A significant and/or complete reduction (100% inhibition) of the F1 progeny of both insects was obtained as a result of parental exposure to the tested botanicals, especially at the highest
doses applied. Botanicals under investigation showed a considerable grain protecting activity against the tested insect species
and could be included in integrated pest management (IPM) strategies. 相似文献
20.
In vitro propagation technique of Balanites aegyptiaca, a multipurpose woody tree was studied. Nodal segments including axillary bud from mature tree were used as an explant and
their morphogenetic potential was tested on MS media with various concentrations (2.5–15.0 μM) of 6-benzyladenine (BA), Kinetin,
and Thidiazuron alone or in combination with different concentrations (0.5–2.5 μM) of α-naphthalene acetic acid (NAA). Nodal
segments showed axillary bud proliferation in almost all media tried. MS medium containing 12.5 μM BA alone was effective
for inducing multiple shoots (5.0 ± 0.22) with an average shoot length (3.7 ± 0.26 cm) in 67% of cultures. A better shoot
differentiation and elongation was achieved in a combined treatment of BA (12.5 μM) and NAA (1.0 μM). Half strength MS medium
supplemented with Indole-3-butyric acid (IBA) gave the best result for rooting. The maximum frequency of root formation (68%),
number of roots (5.3 ± 0.32) and root length (4.1 ± 0.38 cm) was obtained on half strength MS medium containing 1.0 μM IBA.
The regenerated plantlets were potted and acclimatized successfully in a growth chamber and then moved to the greenhouse. 相似文献
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