Effects of stilbenes from bark of <Emphasis Type="Italic">Picea glehnii</Emphasis> (Sieb. et Zucc) and their related compounds against feeding behaviour of <Emphasis Type="Italic">Reticulitermes speratus</Emphasis> (Kolbe)

The effects of stilbene glucosides and related compounds on termite feeding behavior were investigated using paper disc methods against the subterranean termite Reticulitermes speratus. The stilbene-rich fraction and isorhapontin (3-methoxy-3,4, 5-trihydroxystilbene-3--d-glucoside) from bark extracts of Picea glehnii showed avoidance by termites in choice tests. In the no-choice tests using compounds purified from the stilbene-rich fraction, the largest feeding deterrent effect was observed for piceid (3,4,5-trihydroxystilbene-3--d-glucoside), followed by isorhapontin, and astringin (3,3,4,5-tetrahydroxystilbene-3--d-glucoside), at the concentrations from 0.63 to 2.5µmol/disc. No change in activity was observed at retentions of more than 5.0µmol/disc. When the activities of isorhapontin and its aglycone derivative (isorhapontigenin: 3-methoxy-3,4,5-trihydroxystilbene) were compared with that of taxifolin (3,3,4,5,7-pentahydroxyflavanone) in the no-choice test, the stilbenes exhibited a larger antifeedant potential. Methylation of isorhapontigenin increased its termiticidal activity.Part of this study was presented at the 32nd Annual Meeting of the International Research Group on Wood Preservation, 2001     

Absolute configuration of arylglycerol-β-aryl ethers obtained by asymmetric reduction of the correspondingα-ketonic compound with intactFusarium solani cells

When (±)--oxo-guaiacylglycerol--(vanillic acid) ether (1) is degraded byFusarium solani M-13-1, the-ketone is initially reduced to giveerythro andthreo guaiacylglycerol--(vanillic acid) ethers (2), arylglycerol--aryl ethers, both of which are enantiomerically pure. The absolute configuration in each2 was determined by Mosher's method; the products were converted to,-di-(R)--methoxy--trifluoromethylphenylacetates (MTPA esters) (3) oferythro (-)- andthreo (+)-veratrylglycerol--(methyl vanillate) ethers (3), whose¹H nuclear magnetic resonance (NMR) spectra were examined and compared with those of four di-(R)-MTPA ester (3) diastereomers from chemically synthesizederythro (±)-3 andthreo (±)-3. To assign the- and-MTPA-OCH₃ peaks, the¹H NMR scans of several compounds that have substructures of 3 and their 3,4,5-trimethoxyphenyl analogues were examined. When a racemic alcohol reacts with (R)-MTPA to give a pair of (R)-MTPA ester diastereomers, the value was defined as the absolute value of the difference in the¹H chemical shifts of the peak between the diastereomers. It was found that the values of-MTPA-OCH₃ were larger than those of-MTPA-OCH₃ owing to a shielding effect of the veratryl ring located on the-MTPA-OCH₃, and that the-MTPA-OCH₃ peaks in the 3,4,5-trimethoxyphenyl compounds shifted downfield relative to those in the veratryl compounds. On the basis of the¹h NMR data of (R)-MTPA esters, the absolute configuration of the four chemically prepared diastereomers (3) were determined. The catabolicerythro 3 [fromerythro (-)-3] andthreo 3 [fromthreo (+)-3] were identical to (R, S, R)-erythro 3 and (R, S, S)- threo 3, respectively. An hydrogen species in the fungal reduction would attack the-ketone fromre-face of both (R)-1 and (S)-1, givingerythro (S, R)-2 andthreo (S, S)-2, respectively.Part of this paper was presented at the 33rd Lignin Symposium, Tsukuba, November 1988     

5α-Reductase inhibitory component from leaves ofArtocarpus altilis

The inhibitory effects of 17 samples prepared from Thailand plants on 5-reductase activity were examined. The acetone extract of leaves ofArtocarpus altilis showed potent 5-reductase inhibitory activity. Fractionation guided by 5-reductase inhibition led to the isolation of 2-geranyl-2,3,4,4-tetrahydroxydihydrochalcone from the acetone extract of leaves ofA. altilis. This compound showed more potent inhibitory effect (IC₅₀=38M) than-linolenic acid known as a naturally occurring potent inhibitor.     

Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)

Xylan prepared from culms of kumaizasa (Sasa senanensis Rehd.), a representative species of bamboo grass, was hydrolyzed with-xylanase ofStreptomyces olivaceoviridis E-86. Four arabinoxylo-oligosaccharides and two glucuronoxylo-oligosaccharides were isolated from the enzymatic hydrolysate of the xylan by chromatography on a charcoal column, a Dowex 1-x8 column, a Toyo-pearl HW-40S column, and a LiChrospher 100 NH₂ column and on preparative paper chromatography. The results of the structural analyses of the saccharides showed that the isolated oligosaccharides had the structures of 3²--l-arabinofuranosyl-xylobiose, 3²--l-arabinofuranosyl-xylotriose, 3²--[-d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylobiose, 3³--[-d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylotriose, 2³--4-O-methyl-d-glucuronosyl-xylotriose, and 2³--d-glucuronosyl-xylotriose. From the structural analysis of the oligosaccharides derived from the xylan, kumaizasa xylan was concluded to be a kind of arabinoglucuronoxylan having not only stubs of singlel-arabinose and singled-glucuronic acid but also stubs of disaccharide units such as-d-xylopyranosyl-(1 2)-l-arabinofuranose.     

Four glucosides ofp-hydroxyphenyl derivatives from birch leaves

The extractive of shirakamba (Betula platyphylla Sukatchev var.japonica Hara) leaves was investigated. Four glucosides ofp-hydroxyphenyl derivatives were isolated, and their structures were indentified as betuloside (I), 3,4-dihydroxy-propiophenone-3--d-glucopyranoside (II), salidroside (III), and arbutin (IV). Arbutin was newly found in the leaves of shirakamba.     

Two lignan rhamnosides from birch leaves

The extractives of shirakamba (Betula platyphylla Sukatchev var.japonica Hara) leaves were investigated. Two lignan glycosides were isolated, and their structures were elucidated to be 1-(4-hydroxy-3-methoxyphenyl)-2-[1-(3--l-rhamnopyranosyloxypropyl)-3-hydroxyphenoxy]-1,3-propanediol (I) and a new 2,3-dihydro-2-arylbenzofuran configuration neolignan,cis-2,3-dihydro-2-(4--l-rhamnopyranosyloxy-3-methoxyphenyl)-3-hydroxymethyl-7-hydroxy-5-benzofuranpropanol (II). These neolignan rhamnosides were newly found in shirakamba.     

Preparation of liquid polyesterpolyols from glucose and its methyl derivative

Preparation of liquid polyols from d-(+)-glucose (Glc) and its derivative, methyl--d-glucoside (m-Glc), has been studied. Direct reaction of -caprolactone (CL) with Glc (CL:Glc = 2:1–5:1 in weight ratio) at 150°C using tin(II) 2-ethyl hexanoate (SnEht₂) (series A), in which melted Glc was suspended in CL, resulted in a dark-brown coloration of the reactants. The reaction was accompanied by formation of high molecular weight resins, a pH drop, production of water, and a considerable decrease in the hydroxyl value from the theoretically expected one. In the case of Glc/ethylene glycol (EG)/CL reaction system with SnEht₂ catalyst at 150°C (series B), in which the weight ratio of Glc to EG was fixed at 1:1, Glc dissolved in the EG/CL mixture, but the brown coloration of the reactant mixture still occurred. In this case, the formation of water was enhanced, but the other effects found in series A were suppressed to a considerable extent. In the m-Glc/CL/SnEht₂ reaction system (series C), in which m-Glc reacted with two to five times weight amounts of CL under the same conditions adopted in series A, development of the color, the production of high molecular weight materials and water, and the changes in pH and hydroxyl value were not observed. These results are discussed based on the chemical structural differences: Glc exists mostly in the hemiacetal form, but tautomerizes to the aldehyde, whereas m-Glc is an acetal and is protected from reversion to the aldehyde.     

Effect of rhizobial inoculation methods and host plant provenances on nodulation and growth of <Emphasis Type="Italic">Acacia senegal</Emphasis> and <Emphasis Type="Italic">Acacia nilotica</Emphasis>

The purpose of this work was to determine the most efficient methods of inoculation to significantly improve nodulation and growth of Acacia senegal and Acacia nilotica, grown under greenhouse conditions. Our results showed that inoculation using dissolved alginate beads containing rhizobia significantly improved the growth of both acacia species better than the growth of plants in other treatments. The experiment with A. nilotica was conducted in two unsterilised soils from different areas. Plants grown in soil from Bel Air were well-nodulated and showed better growth than plants grown in soil from Sangalkam. However, no difference between these soils was shown between the several methods of inoculation and their effect on the nodulation and growth of plants. An interaction between A. senegal and A. nilotica provenances and the effect of inoculation with rhizobia was also demonstrated. Bel Air provenance of A. senegal, Dahra and RIM provenances of A. nilotica grew best of several provenances tested. These results suggest that (1) it may be possible to improve growth and yield of A. senegal and A. nilotica by careful selection of each symbiotic partner; and (2) nursery-grown seedlings of A. senegal and A. nilotica should be inoculated, just after sowing, with dissolved alginate beads containing a mixture of selected rhizobia. Résumé. Lobject de notre travail était de déterminer la méthodologie dinoculation la plus efficace pour améliorer significativement la nodulation et la croissance dAcacia senegal et dAcacia nilotica poussant dans des conditions de serre. Nos résultats ont montré que linoculation utilisant des billes dalginate dissoutes améliorait significativement la croissance des deux espèces dAcacia par rapport à celle des plantes des autres traitements. Lexpérience avec A. nilotica a été faite dans deux types de sol non stériles. Nous avons observé que les plants poussant dans le sol de Bel Air étaient plus nodulés et mieux développés que ceux poussant dans le sol de Sangalkam. Cependant, aucune différence significative entre les deux sols na été mise en évidence en termes deffet des différentes méthodologies dinoculation sur la nodulation et la croissance des plantes. Il a aussi été démontré lexistence dune interaction entre les provenances dA. senegal et dA. nilotica et leffet de linoculation avec rhizobium. Cest ainsi que les provenances Bel Air dA. senegal, Dahra et RIM dA. nilotica ont le mieux poussé parmi les différentes provenances testées. Lensemble de ces résultats suggèrent: ⁽¹⁾ il devrait être possible daméliorer la croissance et la production dA. senegal et dA. nilotica par une sélection des deux partenaires symbiotiques; ⁽²⁾ Les jeunes plantules dA. senegal et dA. nilotica produites en pépinière devraient être inoculées juste après le semis avec des billes dalginate dissoutes contenant un cocktail de souches sélectionnées.     

A new diterpene dimer from the bark ofChamaecyparis obtusa

This paper describes characterization of diterpenes of the bark of Japanese cypress,Chamaecyparis obtusa (S. and Z.) Endl, without the resinous stem canker to learn the difference between the cypress bark affected with the canker. A diterpene dimer and two diterpenes, 6,12-dihydroxyabieta-8,11,13-trien-7-one and 6,12-dihydroxyabieta-5,8,11, 13-tetraen-7-one, were firstly isolated from Japanese cypress. The dimer, 6-(abieta-6,8,11, 13-tetraenyl-12-oxy)-7-methoxyabieta-8,11,13-trien-12-ol, was a new compound. It is a terpene indicative ofC. obtusa not infected with the resinous stem canker. Five known diterpenes were also isolated.     

Zum Auftreten einiger Laus- und Käferarfen an Jungkiefern

Summary Larvae ofErnobius spp. and adults ofBrachyderes incanus were serious pests of shoots ofPinus silvestris andP. contorta at several localities of Rhine valley and near Meppen/Ems.In the same areas aphids(Lachninae) and their predators were very abundant.Protolachnus agilis Kltb. became very harmful on young pines in experiment field cages where predators had been excluded.Pineus sp. was frequent in the field cages and in young pine plantations near Meppen.

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Résumé En divers endroits de la vallée du Rhin et près de Meppen/Emsland, des larves d'Ernobius spp. et des adultes deBrachydeles incanus furent observés comme étant de sérieux pour les jeunes pousses dePinus silvestris et deP. contorta.Dans les mêmes régions des pucerons(Lachninae) et leurs prédateurs étaient très abondants.Protolachnus agilis Kltb. divint très nuisible aux jeunes pins placés dans des cages expérimentales où les prédateurs avaient été enfermés.Pineus sp. é tait aussi abondant dans cages expérimentales et dans les plantations de jeunes pins près de Meppen.

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Meppen/Emsland Eronobius spp. Brachyderes incanus, Pinus silvestris P. contorta. (Lachninae) Protolachnus agilis Kltb. , - .Pineus sp. - Meppen.

     

The reaction of guaiacol with iron III and chromium VI compounds as a model for wood surface modification

Summary The reaction of ferric chloride with the lignin model guaiacol affords primarily a complex mixture of coupled guaiacol oligomers. Major components were the symmetrical carbon-carbon coupled dimer 3,3-dimethoxy-[1,1-biphenyl]-4,4diol and the trimer 3,3,5-trimethoxy-[1,1:3,1-terphenyl]-4,4,4-triol which were isolated by preparative HPLC and characterized by ¹HNMR spectroscopy and mass spectrometry. An unstable component believed to be a 4,4-diphenoquinone derived from the trimer was also prominent. The reaction of chromium trioxide with guaiacol yields the same dimer, trimer and diphenoquinone as well as 2-methoxy-p-benzoquinone. The major product with chromium trioxide, however, is an inert, highly insoluble polymer which was shown by degradation to contain guaiacol oligomers bound or crosslinked by hydroxylated chromium species. Magnetic susceptibility measurements clearly indicated that the valency of chromium in the polymer was + 3. It is postulated that similar complexes formed from phenolic lignin units are responsible for the weather resistance of chromium trioxide treated wood surfaces. In a broader context this study is relevant to the fixation of hexavalent chromium from a range of widely used wood preservative formulations.The authors wish to thank the following: Tom Syers (CSIRO Division of Forest Products) for technical assistance, Mary Reilly (CSIRO Division of Forest Products) for NMR spectral determinations and Ivan Vit (CSIRO Division of Chemicals and Polymers) for mass spectral determinations     

Cultural factors influencing adventitious shoot and plantlet formation from slash pine cotyledons

Cultural factors affecting in vitro shoot and subsequent plantlet formation of slash pine (Pinus elliotti Engelm.) cotyledons were investigated. Basal media composition, N⁶-benzylaminopurine (BAP) concentration and exposure time significantly influenced bud induction in cotyledons cultured under a continuous photoperiod of 35–40 mol m^–2 s^–1 at 24 ± 1 °C. The largest number of adventitious shoots was obtained after 28 days exposure to 66 M BAP-supplemented modified Gresshoff and Doy 1 (GD1) medium. Relatively high frequencies of large shoots were obtained after a 14-day exposure to 22 M BAP-supplemented Brown and Lawrence (BL) or 66 M BAP-supplemented GD1. Adventitious shoots derived from 21- or 28-day exposures to BAP developed more slowly and were smaller in size than those derived from a 14-day exposure to the cytokinin. Shoot differentiation and subsequent growth were also influenced by basal media, media concentration, and presence of activated charcoal in the medium. The percentage of cotyledons forming shoots was highest on half-strength GD1 medium containing activated charcoal. Rooting was achieved in vitro under a continuous photoperiod of 60–70 mol M^–2 S^–1. Roots were formed when excised shoots were planted on GD 1/2 medium supplemented with 2.68 M 1^–1 a-napthaleneacetic acid (NAA) with or without BAP for 14 days. The proposed technique of slash pine propagation using cotyledon explants can produce up to 100 seedlings per embryo.     

Die neuesten Erkenntnisse der forstlichen Entomologie in der Tschechoslowakei

Summary Present development of forestry entomology in the SSRThis article gives an outline on the occurrence of the more important harmful forest insects in the SSR in the year 1950–1962 and on the results of their study. The Norway spruce suffered from the year 1954 notably byIps typographus L. The control was based on insecticides, studies included optimal density of natural regeneration at the mass propagation etc. It was found, for instance, that typical density borings in bark (151–300 borings per 1 sq.m. of bark) shows variation in the shortest distance between borings from 54–70 mm. Relation between density of borings and their distance indicates function y = a.x^–b, (constant a = 476,9 and constant b= 0,38). It was further found thatIps typographus L. shows even sister generations twice in a year (about 60%, eventually even 30% females of the preceding population). Sister laying is conditioned mainly climatically, i.e. also by the forest stand elevation above sea level. A secure control of further harmful species of Norway spruce,Trypodendron lineatum (Ol.) was carried out by extirpation of wintering beetles in soil, eventually by treating of round wood by emulsions on the HCH + DDT basis.Cephaleia abietis L. shows two diapause periods in a larval stage. For this reason, the development of this species lasts prevailingly two years (90% population). Swarming imagines are controlled by chemical aerosols (10% DDT in the dosis 4–8 kg/ha). On obtained from eggsTrichogramma cephalciae Hochm. et Mart. which cannot be grown on eggs of butterflies. Investigations found that the clean eating ofZeiraphera diniana Guen. begins at the density of 300 caterpillars per one meter of long branch. Chemical control (aerosol 10 l/ha) is recommended when feeding is repeated for three years. The mass propagation ofBupalus piniarinus L. appeared on the Scotch pine. The critical number amounts 400 caterpillars per one kg of needles.Hylobius abletis L. is controlled by insecticides in trap barks and by underground traps for concentration of egg laying. Oak was injuried mostly by the rollerTortrix viridana. The critical number is one caterpillar per one bud. Other, in the SSR new harmful insect of oakArchips crataegana (Hb.) showed the critical number 100 egg heaps on the stem to the height of 2 m. The eggs of the above mentioned species showedTrichogramma embryophagum cacoeciae March., which can easily be grown on butterfly eggs. Oak 60–200 years old was attacked byApethymus biaccatus (Gmel.) andA. abdominalis (Lep.). Silver fir was harmed mainly by the rollerChoristoneura murinana (Hb.). Also the critical number was found. Egg rearing gaveTrichogramma minutum Ril. Ash indicates new type of bark injuries caused probably by a representative of the genusLestes (Fig. 1.). Poplars are damaged by the species of the familyCerambycidae, further byCryptorrhynchus lapathi L. and by the species of the familyAegeridae. Saperda carcharias L. has in the Czechoslovak conditions a generation of three years. Eggs overwinter.Cryptorrhynchus lapathi L. has in the southern Moravia mostly (70% population) one year generation, the rest shows a generation of two years. Beech and hornbeaum were harmfully attacked byErannis aurantiaria Hb.) andColotois pennaria (L.). It is obvious that in the year 1950–1962 several species (Alchips crataegana (Hb.),Apethymus braccatus (Gmel.),A. abdominalis (Lep.) on oak,Erannis aurantiaria (Hb.) andColotois pennaria (L.) on. hornbeam and oak,Pachynematus scutellatus Htg. on Norway spruce etc.) previously not known as important forest injurious insect, appeared as calamity species. Further it is obvious that some harmful insects probably under the influence of changed structure and also forest stand microclimate are retreating whereas other species show mass outbreak. For this reason it is necessary to study the lawfulness of the propagation of the individual species.

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Résumé Les plus récentes expériences de l'entomologie en TchécoslovaquieLe travail contient un aperçu concernant la présence d'insectes nuisibles forestiers de certaine importance en Tchécoslovaquie au cours des années 1950–1962 ainsi que le résultat de leur recherche. Jusqu'à 1954 l'épicéa a été assaili surtout par l'Ips typogiaphus L. On cherche une solution de la défense au moyen des insecticides, la densité optimale d'attaque en cas de la reproduction excessive etc. Par exemple il a été constaté que lors de la densité typique de forage de l'écorce (151–300 forages par mètre carré d'écorce) leur distance ré ciproque varie de 54 à 70 mm. Le rapport entre la densité de forage et de leurs distances présente la forme de fonction y = a.x^–b, où la constante a = 476,9 et la constante b = 0,38. Ensuite il a été constaté qu'en ce qui concerne l'Ips typogiaphus L. existent même deux gé nérations-soeurs au cours de l'année (environ 60 % respectivement encore 30 % des femelles de la population pécédente). La production de pondaisonsoeur est soumis surtout au facteurs climatiques, c'est à dire même á l'altitude des peuplements. Contre un antre parasite de l'épicéa, leTrypodendron lineatum (Ol.), une certaine défense fut élaborée par l'extermination d'imagos hivernant dans le sol ou par le traitement de rondin par des émulsions sur la base HCH + DDT. En ce qui conceme laCephaleia abietis L. on a constaté deux phases de diapause en état de larve. C'est pourquoi le développement duparasite est en majorité biennal (90% de la population). II a été introduit une lutte chimique contre l'essaimage d'imagos par des aerosols (10% de DDT dans une dose de 4 à 8 kg par ha). On a réussi à faire sortir des oeufs de laTrichogramma cephalciae Hochm. et Mart. que l'on ne peut pas cultiver sur les oeufs de papillons. En ce qui concerne laZeiraphera diniana Guen. il fut constaté que la défoliation compléte a lieu par 300 chenilles sur une branche d'un métre de longueur. La lutte chimique est recommandée en cas d'attaque repétée au cours de trois ans. Sur des pins il fut constaté la multiplication de masse duBupalus piniarius L. où le chiffre critique et atteint par 400 chenilles sur un kg d'aiguilles. En ce qui concerne l'Hylobius abietis L. on élabora une manière de défense en utilisant des insecticides dans des pièges d'écorce et des pièges souterrains pour saisir les pondaisons. Sur le chêne c'était laTortrix viridana qui a causé les plus grands dé gâts. Le chiffre critique est exprimé par une chenille pour un bourgeon. En ce qui concerne l'ulté rieur parasite de chêne nouvellement apparu en Tchécoslovaquie, l'Archips crataegana (Hb.), le chiffre critique fut fixé par 100 agglomérations ovulaires sur le tronc jusqu'à 2 mètres de hauteur. On a obtenu laTrichogramma embryophagum cacoeciae March., qui peut être facilement cultivée sur les oeufs de papillons. Sur les chênes d'un âge de 60 à 200 sont apparus ensuite comme parasite desApethymus braccatus (Gmel.) et desApethymus abdominalis (Lep.). Sur le sapin apparaît surtout laChoristoneura murinana (Hb.). Un chiffre critique fut fixé. On a obtenu à partir des oeufs laTrichogramma minutum Ril. On a découvert sur le frêne un nouveau type de lésion de l'é corce provoqué vraisemblablement par la libellule g.Lestes (Fig. 1). Les capricornes, leCryptorrhynchus et lesSesia spp. sont nuisibles aux peupliers. En Tchécoslovaquie laSaperda carcharias L. a une géneration triennale. Les oeufs hivernent. LeCryptorrhynchus lapathi L. a en Moravie du Sud en majorité (70% de la population) une génération annuelle, le restant, biennale. Ensuite sur le hêtre et sur le charme a eu lieu l'apparition désastreuse de la chenille arpenteuseErannis aurantiaria (Hb.) et de laColotois pennaria (L.). Il en ressort donc qu'au cours des années 1950–52 plusieurs espèces ont fait une apparition désastreuse:Archips crataegana (Hb.),Apethymus biaccatus (Gmel.),Apethymus abdominalis (Lep.) sur le chêne,Erannis aurantiaria (Hb.) etColotois pennaria (L.) sur le charme et le hêtre, lePachynematus scutellatus Htg. sur l'épicéa et d'autres qui é taíent auparavent inconnus comme parasites considérables de la forêt.On peut en déduire qu'évidemment sous l'influence de la structure transformée et par consé quent du microclima des peuplements, certains parasites tendent à disparaître, tandis que d'autres insectes deviennent parasites désastreux. C'est pourquoi une recherche approfondie des lois de la reproduction en masse des différentes espèces est nécéssaire.

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- 1950–1962. . 1954. Ips typographus L. , , . . , (151–300 1 ² ) 54 70 . y = a x^–b, a = 476,9 b = 0,38. , Ips typographus L. ( 60%, 30% ). , . . . Trypodendron lineatum (01.) , HCH + DDT. Cephalia abietis (L.) , (90% ). (10% 4–8 (). Trichogramma cephalciae Hochhm. & Mart., . Zeiraphera diniana Guen. 300 1 . . . ( 10 /) . Bupalus piniarius L., 1 400 . Hylobius abietis L. . Tortrix viridana L. 1 1 . , ,Archips crataegana (Hb.) 100 2 . Trichogramma embryophagum cacoeciae March., . 60–200 Apethymus braccatus (Gmel.) Apethymus abdominalis (Lep.). Choristoneura murinana (Hb.). . Trichogramma minutum Ril. - Lestes (. 1). , .Saperda carcharias L. . .Cryptorrhynchus lapathi L. (70% ) , . Erannis aurantiaria (Hb.) Colotois pennaria (L.). , 1950–1962. (Archips crataegana (Hb.),Apethymus braccatus (Gmel.),Apethymus abdominalis (Lep.), Erannis aurantiaria (Hb.) Colotois pennaria (L.), Pachynematus scutellatus Htg. . .), . , , . .

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Herrn Prof. Dipl.-Ing. Dr. A. Pfeffer zum 60. Geburtstag gewidmet.     

Prey consumption by larval and adult stages of the entomophagous ladybird Serangium parcesetosum Sicard (Col., Coccinellidae) of the cotton whitefly, Bemisia tabaci (Genn.) (Hom., Aleyrodidae), at two different temperatures

The entomophagous ladybird, Serangium parcesetosum Sicard (Col., Coccinellidae) appears to be a promising predator against the cotton whitefly, Bemisia tabaci (Genn.) (Hom., Aleyrodidae). Knowledge about its consumption of B. tabaci is incomplete. The present study was undertaken to determine the potential use of this predator in biological control programs. The daily and total prey consumption of S. parcesetosum through the entire development of the larval instars as well as during three different periods of longevity of adult females and males feeding on nymphs or puparia of B. tabaci as prey was examined. In addition, the daily prey consumption of the adult females where the number of B. tabaci puparia was altered was studied at two different temperatures in the laboratory. The results showed that the mean daily prey consumption of nymphs or puparia of B. tabaci by larval instars of S. parcesetosum increased gradually as development progressed at both temperatures. Significant differences were found in the mean total prey consumption among the different predatory larval instars. In total, S. parcesetosum consumed during its entire larval development significantly more nymphs than puparia; at 18°C it consumed a mean of 1,566.1 () and 1,443.9 () nymphs or 280.0 () and 250.8 () puparia, while at 30°C, it consumed a mean of 1,119.1 () and 979.9 () nymphs or 188.2 () and 171.6 () puparia. Over the three studied periods of the adult stage of S. parcesetosum, the mean daily prey consumption by the females and males fluctuated irregularly at both temperatures. At 18°C and 30°C, both females and males consumed significantly more nymphs than puparia. No significant differences were found between females and males with respect to consumption of nymphs, although significant differences were found between the sexes with respect to consumption of puparia, but at 18°C only. The mean total prey consumption over the three studied adult stages was 2,188.4 () and 1,994 () nymphs or 727.1 () and 624.8 () puparia at 18°C, while at 30°C the mean values were 3,947.7 () and 3,577.3 () nymphs or 1,600.5 () and 1,448.8 () puparia. S. parcesetosum adapted smoothly to fluctuating prey availability, where the mean daily prey consumption became higher when 50 puparia/day was offered. In contrast, the predator consumed most individuals when the number of prey supplied was 10 or 5 puparia/day. Prey consumption decreased during the second experimental week in the trial, before which 50 puparia/day had been offered. In the other trials, before which 20, 10 and 5 puparia/day had been offered, a considerable increase in prey consumption was noted.This paper is dedicated to Prof. Dr I. Akif Kansu, University of Ankara, Turkey on his 80th birthday.     

Generationszahl der RübenfliegePegomyia hyoscyami betae Curt.

Summary During the years 1959–1961 the number of generations was followed in Spinach Leaf Miner (Pegomyia hyoscyami betae Curt.) in central Bohemia at 310 m above sea level and in the year 1961 in southern Bohemia at 450 m. The larvae and the eggs were studied on the leaves at regular 10–14 days' intervals by controlling each plant and images by means of a sweeping method. There have been acertained two generations in the year 1959 and three generations in the year 1961 in central Bohemia and two generations in the year 1961 in southern Bohemia. The occurence of every generation of larvae on the suggar-beet was separate and the previous and the consecutive generation did not overlop.The origin of the erroneous date on 6–7 generations of this species (Rambousek, Neuwirth, 1931) has been also cleared up in this article. This statement has been taken over by Sorauer (1953) from the Review of Applied Entomology (1931) and has been lately mentioned by many authors. F. Neuwirth gave a personal information, that the statement on 6–7 generations occurence of Spinach Leaf Miner has been found on the basis of theoretical calculation (from the lenght of one generation in spring and not on the basis of the facts found in natural conditions).

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Résumé En 1959–1961 le nombre des générations de la mouche de la betterave a été observé dans la Bohème Centrale á l'altitude de 310 m audessus de la mer et en 1961 dans le Sud de la Bohème á l'altitude de 450 m. Les larves et les oeufs ont été observé sur les feuilles dans les intervalles de 10–14 jours regulierement effectués sur chaque plante et imagines en particulier par le filet fauchior. En 1959 deux générations et en 1960 et 1961 trios géné rations dans la Bohéme Centrale, ainsi que en 1961 deux générations dans le Sud de la Boheme ont été constates. La prence de chaque génération des larves sur la betterave aété separée et les générations consecutives ne malaient pas presque jamais avec les précedents.Dans cet travail l'auteur a clarifié comment une donnée fautive sur 6–7 générations de cette espéce est survenu (Rambousek et Neuwirth, 1931). Cette donnée orginante de Review of Applied Entomology [1931] a été reprise par Sorauer (1953) et elle a été ensuite récemment mentionée par d'autres auteurs. Selon une déclaration personelle de Mr. F. Neuwirth les donées des 6–7 générations de la mouche de la betterave en Tschécoslovaquie ent été acquis par une évaluation théoretique (se basant sur la durée de la génération du printemps et non sur les resultats des observations réelles dans les conditions naturelles.

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Richtungen der angewandten Entomologie in Bulgarien

Summary For protection of agricultural crops, archards, forests and stored products a system of institutes for vegetative protection have been gradually built in Bulgaria within the last fifteen years. Now a leading part in the study of pests and diseases of plants are taking the Academy of Rural Economy Sciences and the Institutes for Plant Protection, for Forest Protection, the Zoological Institute by the Bulgarian Academy of Science, the Higher Rural Economy Institute and the Higher Forest Institute. Specialists of all ranks are working at these institutes: academicians, member correspondents, professors, senior and junior scientific collaborators and a great number of experts and assistants. Parallel with the studies of specific composition of pestilent insects, their biology is being explored and experiments are being made with insecticides. An inquiry is also being undertaken for utilization biologic combat against noxious insects. In aid of scientific institutes a dense network of services for heading the research work and combat on the terrain has been organized.

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Résumé Pour la défense des cultures agricoles, des vergers, des forêts et des produits récoltés, un système d'instituts pour la défense végétale a été successivement dé veloppé en Bulgarie pendant les dernières quinze années. Actuellement l'Academie des sciences de l'economie rurale et les Instituts pour la défense végétale ont le rôle dirigeant dans les recherches de la nocivité et des maladies sur les plantes. Dans ces instituts travaillent des spécialistes de toutes les catégories scientifiques: des académiciens, des membres-correspondants, des professeurs, des travailleurs scientifiques supérieurs et plus jeunes, avec un grand nombre d'aides-spécialistes. Parallèllement à l'étude de composition specifique des insectes nuisibles on étudie sa biologie, de même on fait des expériences avec des insecticides. Aussi on cherche à utiliser la lutte biologique contre les insectes nuisibles. Pour faciliter les instituts scientifiques dans cette lutte et leur recherche sur le terrain, un immense service est organisé dans tous les plus grands emplacements peuplés du pays.

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Interactions between<Emphasis Type="Italic"> Orthotomicus erosus</Emphasis> (Woll.) (Col., Scolytidae) and the Argentine ant<Emphasis Type="Italic"> Linepithema humile</Emphasis> (Mayr) (Hym., Formicidae)

Interactions occurring between Orthotomicus erosus, an economically important pine bark beetle in Portugal, and the aggressive Argentine ant Linepithema humile were investigated. Tri-trophic experimental enclosures consisting of Pinus pinaster logs, O. erosus adults and L. humile workers were set up in the field and in the laboratory. Interactions between ants and scolytids were observed and quantified in terms of the bark beetles success in colonizing the logs. The presence of L. humile did not influence the ability of O. erosus to colonize the logs, apparently due to the difficulties met by the ants in handling the bark beetles and to their inability to injure them. Thus, although abundant in many pine stands in Portugal and in spite of its aggressiveness, L. humile does not seem to affect populations of O. erosus.     

Fogging can control<Emphasis Type="Italic"> Tetranychus urticae</Emphasis> on greenhouse cucumbers

The effect of a fogging system on interactions between Tetranychus urticae and Phytoseiulus persimilis was studied in two cucumber greenhouses in northern Italy. The greenhouses were divided into two compartments, one (referred to as fog area) was humidified using a fogging system and the other was not humidified (no fog area). Four cultivars were investigated: Akito and Frontera during 2000, Dinero and Jazzer during 2001. Tetranychus urticae was released on a number of plants in both compartments. The release of P. persimilis was planned in both compartments at definite T. urticae thresholds. Phytoseiid releases failed, and the colonization by other predators was scarce.Thus, the effect of the fogging system was evaluated only on T. urticae populations. During the first experimental year, spider mite populations reached low densities in the fog area of both cultivars. Higher densities were found in the no fog area, especially in late June and early July. One year later, spider mites were more abundant in the no fog area of the Dinero cultivar. On the Jazzer cultivar, where most plants were infected by powdery mildew, spider mites were less abundant and differences between the two areas were less marked. Since relative humidity values exceeding 90% (considered as effective on T. urticae) were recorded during the night when the fogging system was not active, we suggest that contact with misty water was the main factor responsible for the reduction of T. urticae populations in the fog area.     

Effect of auxins (IBA and NAA) and season on rooting of juvenile and mature hardwood cuttings of Robinia pseudoacacia and Grewia optiva

Juvenile (2 year old trees) and mature hardwood (15 year old trees) cuttings of Robinia pseudoacacia and Grewia optiva were tested for their capacity to form roots. Cuttings were prepared in spring, monsoon and winter seasons and treated with different concentrations (250, 500 and 750 mg/l) of IBA and NAA. These were planted in a mist chamber maintained at 25±1°C with relative humidity >70%. Juvenile cuttings of both species rooted significantly better than mature hardwood cuttings in all three seasons, and the age effect was more pronounced in auxin treated cuttings. The highest rooting in juvenile (83.3%) and mature (66.6%) cuttings was observed with the NAA (500 mg/l) treatment in R. pseudoacacia during the spring season. In G. optiva, IBA (250 mg/l) in the monsoon season was most effective and yielding a maximum of 80% and 70% rooting in juvenile and mature cuttings, respectively. Auxin treatments also significantly enhanced the number of roots, root length, leaf number and leaf area. Statistical analysis of data revealed that interactions between age, season and treatments were significant at P <0.05 level for R. pseudoacacia and non-significant for G. optiva.     

Micropropagation of western hemlock (Tsuga heterophylla [Raf.] Sarg.) from embryonic explants

An in vitro propagation protocol is described for western hemlock, an important forestry species in Canada. For shoot bud induction, embryonic explants were placed initially on one-third strength Schenk and Hildebrandt medium containing 5 M N⁶-benzyladenine (BA) or 5 M BA in combination with either 5 M kinetin or 5 M 2-isopentenyl adenine for 14 days. The explants were transferred to basal medium without cytokinins for 3 weeks, and then to basal medium containing 0.05% activated charcoal. Elongating shoots were subcultured every 4 weeks on charcoal medium. Shoots, 10 mm in stem height, were rooted either in agar or sterilized peat/perlite (1:1). Up to 70% of the shoots formed roots when they were transferred to the latter, moistened with 1/2 strength Gresshoff and Doy medium containing 5 M -naphthaleneacetic acid. About 90% of the plantlets survived transfer to greenhouse conditions.