共查询到20条相似文献,搜索用时 15 毫秒
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Binding of the Sp1 transcription factor by the human Harvey ras1 proto-oncogene promoter 总被引:34,自引:0,他引:34
S Ishii J T Kadonaga R Tjian J N Brady G T Merlino I Pastan 《Science (New York, N.Y.)》1986,232(4756):1410-1413
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Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression 总被引:72,自引:0,他引:72
B Hoyos D W Ballard E B?hnlein M Siekevitz W C Greene 《Science (New York, N.Y.)》1989,244(4903):457-460
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D Gidoni J T Kadonaga H Barrera-Salda?a K Takahashi P Chambon R Tjian 《Science (New York, N.Y.)》1985,230(4725):511-517
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四环素诱导表达系统(Tet-off/Tet-on系统)是比较成熟的真核生物基因诱导表达系统之一,具有高效、无毒、严密开/关功能的特点。猿猴病毒40T(SV40T)是一种病毒癌蛋白,其与肿瘤抑制蛋白p53和Rb结合,并使之失活,从而消除它们抑制细胞生长的功能,使细胞分裂加速,形成肿瘤。利用Tet-on系统首先稳定筛选获得了表达Tet-on系统调节元件rtTA的阳性细胞CHO-pTet-on,再通过稳定筛选又成功得到导入其反应元件的双阳性细胞CHO-pTet-on-pTRE2-SV40T-Hyg,经强力霉素诱导表达了目的基因SV40T,建立了Tet-on基因诱导表达系统的细胞诱导表达研究平台。 相似文献
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T M Williams D Moolten J Burlein J Romano R Bhaerman A Godillot M Mellon F J Rauscher J A Kant 《Science (New York, N.Y.)》1991,254(5039):1791-1794
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Interaction of a liver-specific nuclear factor with the fibrinogen and alpha 1-antitrypsin promoters 总被引:82,自引:0,他引:82
G Courtois J G Morgan L A Campbell G Fourel G R Crabtree 《Science (New York, N.Y.)》1987,238(4827):688-692
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Sequence-specific binding of human Ets-1 to the T cell receptor alpha gene enhancer 总被引:48,自引:0,他引:48
I C Ho N K Bhat L R Gottschalk T Lindsten C B Thompson T S Papas J M Leiden 《Science (New York, N.Y.)》1990,250(4982):814-818
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Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C 总被引:35,自引:0,他引:35
F Margottin G Dujardin M Gérard J M Egly J Huet A Sentenac 《Science (New York, N.Y.)》1991,251(4992):424-426
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Fine structure genetic analysis of a beta-globin promoter 总被引:120,自引:0,他引:120