Total and water-soluble phosphorus excretion from swine fed low-phytate soybeans

A study was conducted to evaluate the impact of feeding soybean meal (SBM) from low-phytate (LP) or traditional phytate (TP) soybeans on performance and excretions from growing swine. Ninety-six crossbred barrows (initial BW = 18 +/- 0.3 kg) were allocated by BW to 24 pens and fed 1 of 4 treatment diets: TP SBM without supplemental phytase; TP SBM plus 500 phytase units of phytase/kg, as-fed basis [Ronozyme P (CT) 2500; DSM Nutritional Products, Basel, Switzerland]; LP SBM (USDA-ARS breeding line CX1834-1) without supplemental phytase, and LP SBM plus phytase. All diets within a feeding phase were formulated to be isocaloric and have similar available Lys and nonphytin P content. Pens were assigned randomly to treatments at the beginning of each of the 4 feeding phases. An indigestible marker was added to the mash feed. Individual pig weights and fecal samples were collected, and feed disappearance by pen was recorded weekly. No phytase inclusion or SBM source effects were observed for pen ADG, ADFI, or G:F (P > 0.05). Total tract apparent digestibility of DM and OM was not different among treatment groups (P > 0.05). Apparent digestibility of P was greater for pigs fed diets containing the LP SBM (48.9 vs. 42.4%; P < 0.01) and less when diets included phytase (44.3 vs. 47.0%; P < 0.0001). Total P (tP) and water-soluble P (WSP) excreted were affected by dietary treatment (tP: 20.0, 18.0, 16.8, and 13.8 g/kg of feces DM, P < 0.01; and WSP: 10.9, 10.1, 9.1, and 8.5 g/kg, P < 0.01, for TP SBM without supplemental phytase, TP SBM plus 500 phytase units of phytase/kg, LP SBM without supplemental phytase, and LP SBM plus phytase diets, respectively). Inclusion of phytase decreased tP and WSP excreted (P < 0.01), as did use of LP SBM (P < 0.01). Diet effects on the fraction of excreted tP that was WSP were observed (P < 0.01); however, there was not a significant effect of SBM source. Inclusion of exogenous phytase in diets increased the proportion of tP that was excreted as WSP from 55% in diets without phytase to 59% in diets containing phytase. The findings suggest that there is a need for LP soybeans as a dietary component to minimize environmental impacts.     

Determination of true digestive utilization of phosphorus and the endogenous phosphorus outputs associated with soybean meal for growing pigs

The objectives of this study were to determine true P digestibility, the gastrointestinal endogenous P outputs associated with soybean meal (SBM), and the role of the large intestine in P digestion in growing pigs. Four Yorkshire barrows, with average initial and final BW of 40 and 58 kg, were fitted with a simple T-cannula at the distal ileum and fed four diets according to a 4 x 4 Latin square design. The diets were cornstarch-based and contained four levels of P (0.098, 0.196, 0.293, and 0.391% on a DM basis) from solvent-extracted conventional SBM. Chromic oxide (3.5 g/kg of diet, as-fed basis) was included as a digestibility marker. Each experimental period consisted of 8 d with a 4-d adaptation period and a 4-d collection of representative ileal digesta (2 d) and fecal (2 d) samples. True ileal and fecal P digestibility values and the ileal and fecal endogenous P outputs associated with SBM were determined by the regression analysis technique. There were no differences (P > 0.05) in true P digestibility values (ileal, 59.0 +/- 8.3 vs. fecal, 51.3 +/- 7.9%, n = 16) and endogenous P outputs (ileal, 0.59 +/- 0.18 vs. fecal, 0.45 +/- 0.21 g/kg of DMI, n = 16) between the ileal and the fecal levels. The endogenous fecal P loss accounted for 8.1 and 17.6% of the NRC (1998) recommended total and available P requirements in growing pigs, respectively. In conclusion, approximately 51% of the total P in conventional SBM is digested in growing pigs. The large intestine does not play an important role in the digestion of P associated with SBM in the growing pig. The fecal loss of the gastrointestinal endogenous P is an important route of P excretion in the growing pig.     

Digestibility of phosphorus in growing pigs as influenced by source and concentration of dietary phosphorus and collection site

The current study examined the influence of source and concentration of dietary phosphorus (P) on the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of P in growing pigs. Eighteen cannulated barrows (25 ± 5 kg) were allotted to a triplicate 6 × 3 incomplete Latin square design with six diets and three periods. The diets comprised of soybean meal (SBM) or distillers' dried grain with solubles ( DDGS) as sole sources of dietary P, and three concentrations of P (2.0, 2.5 or 3.0 g/kg) in a 2 × 3 factorial arrangement. The AID and ATTD of P were not different (p = 0.37) in all diets. Apparent digestibility of P was affected (p < 0.01) by source and concentration of P. There was no interaction between source and concentration of dietary P on the apparent digestibility of P. Determined by regression analysis, the true ileal digestibility of P was 58.3% or 57.6%, and true total tract digestibility of P was 56.0% or 62.6%, for SBM or DDGS, respectively. The regression-derived ileal endogenous P loss (EPL) was 0.61 or 0.13 g/kg DM intake, and total tract EPL was 0.53 or 0.35 g/kg DM intake, for SBM or DDGS, respectively. In conclusion, the study demonstrated that both the source and concentration of dietary P affect the digestibility of P in growing pigs.     

Effect of low-phytate barley or phytase supplementation to a barley-soybean meal diet on phosphorus retention and excretion by grower pigs

Two studies were conducted to determine the effect of diets containing low-phytate barley or supplemented with phytase on P balance and excretion in grower pigs. In Exp. 1, eight 32-kg barrows were assigned to a repeated, 4 x 4 Latin square design and fed 4 diets that contained 96% barley: normal-phytate hulled barley (HB), low-phytate hulled barley (LPHB), normal-phytate hull-less barley (HLB), and low-phytate hull-less barley (LPHLB). The barley cultivars contained 0.16, 0.05, 0.24, and 0.03% phytate, respectively. Inorganic P (iP) was added to the HB and HLB diets to meet the 1998 National Research Council recommendation of available P (aP, 0.23%), whereas LPHB and LPHLB contained sufficient aP. The diets were fed at 2.5 times the maintenance requirement for ME. The apparent total tract digestibilities (ATTD) of P did not differ between the hulled and hull-less barley diets, but P retention (%) and excretion were greater in pigs fed the hull-less barley diets (P < 0.05). The ATTD of P was greater and P excretion was 35% lower in pigs fed the low-phytate compared with the normal-phytate diets (P < 0.001). The amount of P retained (g/d) was greater (P < 0.001) in pigs fed low-phytate barley, reflecting an ATTD of P of 65 and 49% for low-phytate and normal-phytate barley, respectively (P < 0.001). In Exp. 2, eight 21-kg barrows were assigned to a repeated, 4 x 4 Latin square design and fed 4 diets based on barley and soybean meal (SBM): HB-SBM, HB-SBM + iP, HB-SBM + phytase, and LPHB-SBM. The HB-SBM and HB-SBM + phytase diets were deficient in aP, whereas the HB-SBM + iP and LPHB-SBM diets had adequate aP. The feeding regimen was similar to that of Exp. 1. Adding iP to the HB-SBM diet did not affect the ATTD but increased the amount of P retained (g/d) and excreted (P < 0.001). The ATTD and amount of P retained (g/d) did not differ among pigs fed the HB-SBM + iP, HB-SBM + phytase, and LPHB-SBM diets. However, pigs fed the HB-SBM + phytase and LPHB-SBM diets excreted 32 and 29% less P, respectively, than pigs fed the HB-SBM + iP diet (P < 0.05), confirming that low-phytate barley is as effective as supplemental phytase in improving P digestibility and utilization and decreasing P excretion in grower pigs.     

The evaluation of soybean meals from 3 major soybean-producing countries on productive performance and feeding value of pig diets

This study was conducted to evaluate 1) the effects of soybean meal (SBM) from 3 major SBM-producing countries (United States, Brazil, and India) on the growth performance, nutrient digestibility, and meat quality of growing-finishing pigs and 2) the feeding value of the evaluated SBM as a feedstuff for growing-finishing pigs. Soybean meal representative of each country was purchased on the open market. A total of 144 barrows with the average BW of 23.77 (SD = 1.52 kg) were allotted to 3 dietary treatments with 12 replicate pens per treatment and 4 pigs per pen. The US SBM used in this trial had greater CP and total AA contents, greater KOH protein solubility, and less crude fiber than SBM from either Brazil or India. Diets were formulated to be isolysinic and isocaloric. Pigs fed diets containing US SBM were heavier (P < 0.05) than pigs fed diets containing Brazilian or Indian SBM at 12 and 18 wk. Pigs fed diets containing US SBM had greater (P < 0.05) ADG and G:F throughout the overall period when compared with pigs fed SBM produced in Brazil or India. The DM and N digestibility were greater for US SBM (P < 0.05) than Brazilian and India SBM at 6, 12, and 18 wk. Pigs were slaughtered at the end of 18 wk, and no differences were observed on meat quality (backfat thickness, LM area, pH, meat color, water holding capacity, and drip loss) among the treatments, except lean percentages were less and backfat thickness was greater in pigs fed Brazilian and Indian SBM than US SBM. The cost per kilogram of BW gain of the experimental diet containing US SBM was less than that of diets containing Brazilian or Indian SBM, but it would depend on the relative prices of each SBM. Based on the results of the current experiment, the productivity and feeding value of US SBM seems to be greater than the SBM produced in Brazil and India.     

The significance of small vs large intestinal digestion of cereal grain and oilseed protein in the equine

Mature ponies fitted with permanent ileal cannulas were used in two 3×3 Latin square experiments to quantify prececal, postileal and total tract digestion of N. In trial 1, corn, oats and sorghum were each fed with coastal Bermuda grass hay in 75:25 ratios. Apparent prececal digestibilities were similar (P>.05) and averaged 46.6%. By-difference procedures were employed to calculate digestibility of the cereal grain N only and apparent prececal N digestibility averaged 57.1%. In trial 2, a basal corn and hay diet was supplemented with cottonseed meal and soybean meal. Apparent total tract N digestibilities were similar (P>.05) across treatments, and prececal digestibility averaged 45.6%. By-difference calculations were used to determine digestibility of SBM and CSM N alone. Apparent prececal digestibility of SBM was 52.5% and was lower (P<.05) than 81.2% for CSM. It is possible that inadequate or excessive heat treatment of SBM affected enzymatic digestion. True digestibility of total rations fed in trial 2 was estimated by linear regression of nitrogen digested on nitrogen intake or N presented to the large intestine. True N digestibility of diets containing SBM and CSM was 54.7% and 69.4%, respectively.     

Nutritional evaluation of high-digestible sorghum for pigs and broiler chicks

Two experiments were conducted to evaluate the nutritional quality of 2 varieties of Purdue high-digestible sorghum (PHD1 and PHD2) and a normal sorghum, compared with corn, in diets of pigs and broiler chicks. In Exp. 1, 12 pigs (average BW, 55 kg) fitted with ileal T-cannula were fed 4 diets containing 946 g of corn or sorghum (PHD1, PHD2, and normal) per kg in a 2-period crossover design (i.e., each pig received 2 diets over a 2-wk period with 6 pigs per dietary treatment) to determine apparent ileal or total tract digestibility of nutrients and energy. There was no difference in the ileal or total tract digestibility of DM, energy, P, Ca, or N among dietary treatments. In Exp. 2, a total of 192 broiler chicks were grouped by weight into 8 blocks of 4 cages each with 6 chicks per cage, and cages were assigned randomly to 1 of the 4 dietary treatments within each block. Chicks were fed corn-soybean meal (SBM) or sorghum-SBM diets for 21 d to determine apparent total tract retention and then switched to diets containing 935 g of the corresponding corn or 1 of the 3 sorghum varieties per kg for 7 d to determine apparent ileal digestibility and total tract retention. Apparent ileal digestibilities of DM and P, as well as energy, were not different in chicks fed diets containing 935 g of corn or 1 of the 3 sorghum varieties per kg. However, apparent total tract retention of DM, energy, and N in chicks fed corn was greater (P < 0.05) than those fed 1 of the 3 sorghum varieties. Although the apparent ME content of corn was greater than PHD1 and normal sorghum (P < 0.01), it was not different from PHD2 sorghum. There was no difference in apparent total tract retention of DM between chicks fed the corn-SBM and PHD-SBM diets, but it was greater (P < 0.05) in chicks fed the corn-SBM diet than those fed the normal sorghum-SBM diet. Apparent total tract retention of N in chicks fed the PHD1-SBM diet was lower (P < 0.05) than in those fed the corn-SBM diet but greater (P < 0.05) than in chicks fed the normal sorghum-SBM meal diet. No difference in the apparent ME content between the corn-SBM and PHD2-SBM diets was observed, but it was greater (P < 0.05) for the corn-SBM diet than the PHD1- or normal sorghum-SBM diet. Weight gain, feed intake, and feed efficiency were not different in chicks fed the corn-SBM or sorghum-SBM diets. Sorghum could serve as a substitute for corn in cereal grain-SBM diets for pigs and broiler chicks.     

Energy, phosphorus, and amino acid digestibility of high-protein distillers dried grains and corn germ fed to growing pigs

Three experiments were conducted to measure energy, P, and AA digestibility in 2 novel co-products from the ethanol industry [i.e., high-protein distillers dried grains (HP DDG) and corn germ]. These products are produced by dehulling and degerming corn before it enters the fermentation process. Experiment 1 was an energy balance experiment conducted to measure DE and ME in HP DDG, corn germ, and corn. Six growing pigs (initial BW, 48.9 +/- 1.99 kg) were placed in metabolism cages and fed diets based on corn, corn and HP DDG, or corn and corn germ. Pigs were allotted to a replicated, 3 x 3 Latin square design. The DE and ME in corn (4,056 and 3,972 kcal/kg of DM, respectively) did not differ from the DE and ME in corn germ (3,979 and 3,866 kcal/kg of DM, respectively). However, HP DDG contained more (P < 0.05) energy (4,763 kcal of DE/kg of DM and 4,476 kcal of ME/kg of DM) than corn or corn germ. Experiment 2 was conducted to measure apparent total tract digestibility (ATTD) and true total tract digestibility of P in HP DDG and corn germ. Thirty growing pigs (initial BW, 33.2 +/- 7.18 kg) were placed in metabolism cages and fed a diet based on HP DDG or corn germ. A P-free diet was used to measure endogenous P losses. Pigs were assigned to treatments in a randomized complete block design, with 10 replications per treatment. The ATTD and the retention of P were calculated for the diets containing HP DDG and corn germ, and the endogenous loss of P was estimated from pigs fed the P-free diet. The ATTD was lower (P < 0.05) in corn germ (28.6%) than in the HP DDG (59.6%). The retention of P was also lower (P < 0.05) in pigs fed corn germ (26.7%) than in pigs fed HP DDG (58.9%). The endogenous loss of P was estimated to be 211 +/- 39 mg per kg of DMI. The true total tract digestibility of P for HP DDG and corn germ was calculated to be 69.3 and 33.7%, respectively. In Exp. 3, apparent ileal digestibility and standardized ileal digestibility values of CP and AA in HP DDG and corn germ were measured using 6 growing pigs (initial BW, 78.2 +/- 11.4 kg) allotted to a replicated, 3 x 3 Latin square design. The apparent ileal digestibility for CP and all AA except Arg and Pro, and the standardized ileal digestibility for CP and all AA except Arg, Lys, Gly, and Pro were greater (P < 0.05) in HP DDG than in corn germ. It was concluded that HP DDG has a greater digestibility of energy, P, and most AA than corn germ.     

Evaluation of NutriDense low-phytate corn and added fat in growing and finishing swine diets

Two experiments were conducted to evaluate the effects of NutriDense low-phytate corn in conjunction with increasing added dietary fat on growing and finishing pig performance. Diets in both experiments were corn-soybean meal-based, with yellow dent or NutriDense low-phytate corn and 0, 3, or 6% added choice white grease arranged in a 2 x 3 factorial design. There were 25 to 28 pigs per pen and 7 pens (replications) per treatment in both experiments. In Exp. 1, a total of 1,162 gilts with an initial BW of 44.6 kg were used in a 28-d growth study. A constant true ileal digestible (TID) Lys:ME ratio of 2.80 g/Mcal and available P:ME ratio of 0.90 g/Mcal were maintained in all treatment diets. Overall (d 0 to 28), there were no corn source x added fat interactions (P >/= 0.79). Regardless of corn source, ADG and G:F increased (linear, P = 0.03) with increasing added fat. There were no differences (P >/= 0.34) in pig growth performance between those fed NutriDense low-phytate or yellow dent corn. In Exp. 2, a total of 1,128 gilts with an initial BW of 81.6 kg were used in a 28-d growth study. A constant TID Lys:ME ratio of 2.15 g/Mcal of ME and available P:ME ratio of 0.75 g/Mcal were maintained in all treatment diets. Overall (d 0 to 28), there was a tendency (P = 0.07) for a corn source x added fat interaction for G:F, which can be explained by the improved G:F in pigs fed yellow dent corn only when 6% fat was added to the diet, whereas G:F was improved at both 3 and 6% added fat in pigs fed NutriDense low-phytate corn. There were no differences (P >/= 0.18) in growth performance between pigs fed NutriDense low-phytate or yellow dent corn. These results indicate that increasing added fat improved growth performance regardless of the corn source. In addition, growth performance was similar for pigs fed NutriDense low-phytate or yellow dent corn.     

生长猪基础饲粮组成对磷酸氢钙和磷酸二氢钙中磷的全肠道真消化率的影响

本试验旨在研究生长猪基础饲粮组成对磷酸氢钙(DCP)和磷酸二氢钙(MCP)中磷的全肠道真消化率(TTTD)的影响。试验1选用10头平均体重为(30.4±1.8)kg的生长猪,按照10×8不完全拉丁方设计,分别饲喂含有5个DCP添加水平的玉米-豆粕型和含有5个DCP添加水平的小麦-豆粕型饲粮,进行8期消化试验;试验2选用10头平均体重为(30.9±1.5)kg的生长猪,按照10×8不完全拉丁方设计,分别饲喂含有5个M CP添加水平的玉米-豆粕型和含有5个MCP添加水平的小麦-豆粕型饲粮,进行8期消化试验。每期消化试验包括5 d的饲粮适应期和2 d的粪便收集期。结果表明:1)玉米-豆粕型饲粮的总粪磷排泄量极显著高于小麦-豆粕型饲粮(P0.01),小麦-豆粕型饲粮的全肠道可消化磷含量和磷的表观全肠道消化率(ATTD)极显著高于玉米-豆粕型饲粮(P0.01)。饲粮添加DCP和MCP线性增加总粪磷排泄量、全肠道可消化磷含量及磷的ATTD(P0.01)。2)通过使用线性回归法,测得生长猪采食玉米-豆粕型和小麦-豆粕型饲粮对DCP中磷的TTTD分别为82.33%和82.88%,生长猪采食玉米-豆粕型和小麦-豆粕型饲粮对MCP中磷的TTTD分别为85.88%和84.62%。由此可见,生长猪基础饲粮组成对DCP和MCP中磷的TTTD无显著影响。     

Effects of level of supplemental phytase on ileal digestibility of amino acids, calcium, and phosphorus in dehulled soybean meal for growing pigs.

Ileally cannulated pigs were used to assess the effects of four dietary levels of microbial phytase (Natuphos) on the apparent and true digestibility of Ca, P, CP, and AA in dehulled soybean meal. Fourteen pigs (25 kg initial BW) were surgically fitted with T-cannulas at the terminal ileum and assigned to diets in a replicated 7 x 7 Latin square design. Following a 14-d recovery, four diets consisting of 30.5% soybean meal with 0, 500, 1,000, or 1,500 units of phytase/kg of diet were fed. Diets 5 (1.05% lysine, 0.90% Ca, and 0.75% P) and 6 (1.05% lysine, 0.90% Ca, and 0.75% P) contained 35.25% soybean meal and 27.0% soy protein concentrate, respectively. Diet 7 (0.37% lysine, 0.03% Ca, and 0.05% P) was a low-CP, casein-based diet used to estimate the nonspecific endogenous losses of Ca, P, CP, and AA in order to estimate the true digestibility of these nutrients. All diets contained cornstarch and dextrose and were fortified with vitamins and minerals. Chromic oxide was used as an indigestible indicator. The diets were fed daily at 9% of metabolic BW (BW0.75). Apparent and true ileal digestibility of P increased quadratically (P < 0.01) and true digestibility of Ca increased linearly (P < 0.07) with increasing levels of phytase. Apparent digestibility of Ca was unaffected (P = 0.15) by phytase level. Apparent and true ileal digestibility of CP and most AA increased slightly with the addition of 500 units of phytase/kg of diet, but not at higher levels of phytase supplementation (in most cases, cubic effect, P < 0.05). Apparent and true ileal nutrient digestibility coefficients were unaffected by soybean meal source (Diet 1 vs Diet 5), except for arginine and Ca. The apparent and true digestibility coefficients for most of the AA tended (P < 0.10) to be lower in diets containing soy protein concentrate vs the common source of soybean meal used in Diet 5, but ileal digestibilities of Ca and P were unaffected (P = 0.15). In this study, supplemental microbial phytase did not improve the utilization of AA provided by soybean meal but was an effective means of improving Ca and P utilization by growing swine fed soybean meal-based diets.     

Digestibility of phosphorus by growing pigs of fermented and conventional soybean meal without and with microbial phytase

An experiment was conducted to test the hypothesis that the apparent total tract digestibility (ATTD) and the standardized total tract digestibility (STTD) of P in fermented soybean meal (FSBM) are greater than in conventional soybean meal (SBM-CV) when fed to growing pigs. Four diets were formulated to contain FSBM or SBM-CV and either 0 or 800 units/kg of microbial phytase. The only sources of P in these diets were FSBM and SBM-CV. A P-free diet to estimate basal endogenous losses of P was also formulated. Thirty barrows (initial BW: 14.0 ± 2.3 kg) were placed in metabolism cages and allotted to 5 diets in a randomized complete block design with 6 pigs per diet. Feces were collected for 5 d after a 5-d adaptation period. All samples of ingredients, diets, and feces were analyzed for P, and values for ATTD and STTD of P were calculated. Results indicated that the basal endogenous P losses were 187 mg/kg of DMI. As phytase was added to the diet, the ATTD and STTD of P increased (P < 0.01) from 60.9 to 67.5% and from 65.5 to 71.9%, respectively, in pigs fed FSMB. Likewise, addition of phytase to SBM-CV increased (P < 0.01) the ATTD and STTD of P from 41.6 to 66.2% and from 46.1 to 71.4%, respectively. The ATTD and STTD of P were greater (P < 0.01) in FSBM than in SBM-CV when no phytase was used, but that was not observed when phytase was added to the diet (soybean meal × phytase interaction, P < 0.01). In conclusion, the ATTD and STTD of P in FSBM was greater than SBM-CV when no microbial phytase was added, but when phytase was added to the diets, no differences between FSBM and SBM-CV were observed in the ATTD and STTD of P.     

猪的生长阶段对饲料磷的全肠道消化率的影响

本试验旨在研究猪的生长阶段对饲料磷的全肠道消化率的影响。选用初始平均体重分别为27.2、59.1、103.1kg的生长育肥猪各16头,根据体重随机分为低磷饲粮组和高磷饲粮组,每组8个重复,每个重复1头猪。低磷饲粮为无磷酸氢钙(DCP)的玉米-豆粕型饲粮,饲粮总磷浓度为3.3 g/kg,在低磷饲粮中添加14.5g/kgDCP构成总磷浓度为5.9 g/kg的高磷饲粮。采用全收粪法测定总磷的表观全肠道消化率(ATTD),采用差量法计算DCP中磷的全肠道真消化率(TTTD)。试验共12 d,包括5 d适应期和7 d粪样收集期。结果表明:高磷饲粮组的可消化磷含量和总磷的ATTD均显著高于低磷饲粮组(P<0.05);饲喂高磷饲粮时,不同生长阶段猪磷的ATTD无显著差异(P>0.1);饲喂低磷饲粮时,育肥猪磷的ATTD显著高于生长猪和生长育肥猪(P<0.05);虽然饲粮总磷的ATTD受生长阶段影响(P<0.05),但生长阶段对DCP中磷的TTTD无显著影响(P>0.1)。由此可见,猪的生长阶段显著影响植物性饲料原料中磷的消化利用率,但对DCP中磷的消化利用率无显著影响。     

Comparison of the regression analysis technique and the substitution method for the determination of true phosphorus digestibility and faecal endogenous phosphorus losses associated with feed ingredients for growing pigs

Two digestibility experiments were carried out to comparatively measure true phosphorus (P) digestibility and the faecal endogenous P loss associated with soybean meal (SBM) and wheat middling meal (WM) for growing pigs by the simple linear regression analysis technique and the substitution method. Eight barrows, with an average initial body weight 21 kg, were randomly divided into two groups of four pigs with each group being fed four test diets according to a 4 × 4 Latin square design. Eight maize starch-based diets containing four levels (on dry matter basis) of P (0.20, 0.24, 0.36 and 0.42%) and P (0.181, 0.26, 0.36, and 0.43%) were formulated from SBM and WM as a sole source of P, respectively. Chromic oxide (0.30%) was used as a digestibility marker. Each experimental period comprised 8 d with a 4-d adaptation and a 4-d collection of faecal samples. Levels of the assay ingredient substitutions had no effects (P > 0.05) on true P digestibility and the endogenous P loss associated with SBM and WM measured by the substitution method. There were no differences (P > 0.05) between the regression technique and the substitution method in measuring the true P digestibility (SBM: 49.4 ± 3.51 vs. 50.6 ± 2.4; WM: 63.7 ± 5.0 vs. 63.2 ± 5.4%) as well as the faecal endogenous P loss (SBM: 0.62 ± 0.10 vs. 0.71 ± 0.11; WM: 0.65 ± 0.16 vs. 0.70 ± 0.17 g/kg dry matter intake) for the growing pig. Thus, true P digestibility and the faecal endogenous P loss in feed ingredients for pigs may be measured by using the regression analysis technique and the substitution method.     

生长猪饲粮磷水平对磷酸氢钙中磷真消化率评定的影响

本试验旨在研究生长猪饲粮磷水平对磷酸氢钙(DCP)中磷的全肠道真消化率评定的影响。选用56头体重为(25.90±0.25)kg的杜×长×大杂交去势公猪,根据体重随机分为7个处理组,每组8个重复,每个重复1头猪。对照组饲喂未添加DCP的基础饲粮,6个处理组在基础饲粮中分别添加0.64%、1.28%、1.92%、2.56%、3.20%、3.84%DCP。使用全收粪法测定磷的表观全肠道消化率。结果表明:生长猪总磷的表观全肠道消化率随饲粮DCP水平提高呈线性和二次增加(P0.05);随着DCP添加水平的提高,由差量法测得无机磷的全肠道真消化率呈二次上升的趋势(P0.1),具体表现为无机磷的全肠道真消化率随饲粮DCP水平提高呈现出先增加后降低的趋势;通过线性回归法发现,生长猪饲喂低DCP饲粮后测得磷的全肠道真消化率显著高于饲喂高DCP饲粮(P0.05)。由此可见,饲粮磷水平显著影响差量法和线性回归法测得DCP的全肠道真消化率;使用差量法测定饲粮原料磷的全肠道真消化率时应将饲粮磷水平维持在接近磷需要量的水平,使用线性回归法测定磷的全肠道真消化率时应将饲粮磷水平控制在低于磷需要量的范围内。     

Amino acid digestibility in dry extruded-expelled soybean meal fed to pigs and poultry

The digestibility of AA in dry extruded-expelled soybean meal (DESBM) and regular, solvent-extracted soybean meal (SBM) were determined in pigs and poultry. In the pig assay, 4 Cotswold barrows (average initial BW of 80.4 kg) fitted with a T-cannula at the distal ileum were allotted to 4 semipurified diets in a 4 x 4 Latin square design. Diet 1, a low protein diet (5% casein), was used to quantify endogenous CP and AA losses. Diets 2, 3, and 4 were formulated to contain 35% regular, solvent-extracted SBM; batch 1 of DESBM (DESBM-1); and batch 2 of DESBM (DESBM-2), respectively, as the sole source of protein. The DESBM samples were obtained from 2 different batches but were subjected to the same processing conditions. Chromic oxide (0.3%) was included as a digestibility marker in all diets. Compared with DESBM-1 and DESBM-2, apparent ileal digestibility of DM in SBM was greater (P < 0.05). Apparent and true ileal digestibilities of AA in SBM were greater (P < 0.05) compared with DESBM-2. In the poultry assay, 4 dietary treatments were each assigned to adult cecectomized roosters in a completely randomized design. Treatment 1 was a nonnitrogenous diet (NND; 90% sucrose and 10% vegetable oil) used to estimate endogenous N and AA losses. Treatments 2, 3, and 4 contained SBM, DESBM-1, and DESBM-2 as the only source of protein. Each of these diets was fed in 25-g quantities formulated to provide 5 g of CP from the respective soybean meal source. The SBM had greater (P < or = 0.05) true digestibility for isoleucine, leucine, cysteine, proline, serine, and tyrosine compared with DESBM-1. The results indicate that, relative to regular, solvent-extracted soybean meal, AA digestibilities of different batches of dry extruded-expelled soybean meal varied in pigs and poultry.     

Estimation of endogenous phosphorus loss in growing and finishing pigs fed semi-purified diets

Thirty-six barrows were used in a series of 3 P-balance experiments in which growing and finishing pigs were fed highly digestible, semi-purified diets at or below the dietary available P requirement to estimate the effect of BW on endogenous P loss. Experiments 1, 2, and 3 were conducted with pigs averaging 27, 59, and 98 kg of BW, respectively. In each experiment, pigs were placed in metabolism crates and allotted by weight and litter to 3 dietary treatments. The basal diet consisted of sucrose, dextrose, cornstarch, and casein fortified with minerals (except P) and vitamins. Diets 1, 2, and 3 in Exp. 1 were the basal diet with 0, 0.078, or 0.157% added P, respectively, from monosodium phosphate. In Exp. 2 and 3, diets 1, 2, and 3 were the basal diet with 0, 0.067, and 0.134% added P, respectively, from monosodium phosphate. Within replicate, pigs were fed equal amounts of feed twice daily. Pigs were adjusted to treatments for 7 d before a 6-d, marker-to-marker collection of feces and urine. Phosphorus intakes for pigs fed the 3 diets ranged from 1.73 to 3.91 g/d in Exp. 1, from 2.18 to 5.32 g/d in Exp. 2, and from 1.96 to 6.26 g/d in Exp. 3. Fecal P excretion and P absorption increased linearly (P < 0.05) with increasing P intake. In the 3 experiments, urinary P excretion (g/d) was low for pigs fed diet 1 (0.010, 0.011, 0.019) and diet 2 (0.013, 0.058, 0.084) and was low for pigs fed diet 3 in Exp. 1 (0.037); however, urinary P was greater in pigs fed diet 3 in Exp. 2 and 3 (0.550 and 0.486, respectively). When P absorption (Y, g/d) was regressed on P intake (X, g/d) in Exp. 1, 2, and 3, the relationships were linear (P < 0.01): Y = -0.110 + 0.971X (R2 = 0.999), Y = -0.156 + 0.939X (R2 = 0.998), and Y = -0.226 + 0.8919X (R2 = 0.982), respectively. Thus, our estimates of endogenous P loss at zero P intake were 110, 156, and 226 mg/d for 27-, 59-, and 98-kg pigs, respectively. When these Y-intercepts were regressed on BW, the relationship was Y = 63.06 + 1.632X (R2 = 0.996), where Y = endogenous P loss in mg/d and X = BW in kg. Based on these data, we estimate the endogenous P loss of pigs fed highly digestible, semi-purified diets to increase by approximately 1.632 mg for each 1-kg increase in BW from 25 to 100 kg.     

Evaluation of low-oligosaccharide, low-phytate whole soybeans and soybean meal in canine foods

Eight mature dogs (19.3 +/- 0.1 kg) were used in an experiment to compare the effects of feeding soybeans containing low concentrations of oligosaccharides and phytate on nutrient availability in complete foods fed to dogs. All foods were formulated to be isonitrogenous and contained low-oligosaccharide, low-phytate soybean meal (LLM); conventional soybean meal (SBM); low-oligosaccharide, low-phytate whole soybeans (LLB); or conventional whole soybeans (WSB) as the protein source. Daily DMI averaged 287 +/- 4 g/d. Fecal outputs were greatest for LLB and WSB, averaging 48.2 g DM/d. Small intestinal DM digestibility ranged from 80.9% (LLM) to 74.0% (LLB) but was unaffected by treatment. Large intestinal DM digestibility did not differ among treatments (P = 0.652). Total-tract DM digestibility was higher (P = 0.020) for LLM (87.0%) than for SBM (84.8%). No difference in total-tract DM digestibility was observed for the two WSB foods (average = 83.3%; P = 0.286). Nitrogen retention did not differ among foods containing LLM and SBM (1.2 g of N/d; P = 0.486) or LLB and WSB (0.9 g of N/d; P = 0.225). Small intestinal N digestibility did not differ among LLM- and SBM-containing foods (80.6%; P = 0.190) or LLB and WSB (69.3%; P = 0.640). Total-tract N digestibility did not differ among foods containing LLM and SBM (83.5%; P = 0.627) or LLB and WSB (76.8%; P = 0.968). Tryptophan digestibility was higher for SBM than for LLM (P = 0.039). Histidine and tryptophan digestibilities were higher in WSB compared with LLB (P = 0.049 and P < 0.001, respectively). No differences in nonessential AA digestibility were observed when comparing soy-based foods. The results of this study demonstrate that LLM, SBM, LLB, and WSB can be effective sources of protein for canine foods and have a high digestibility. Differences in small intestinal digestibility of tryptophan and histidine may require consideration when formulating diets using low-oligosaccharide, low-phytate soybeans or meal.     

Energy concentration and phosphorus digestibility in whey powder, whey permeate, and low-ash whey permeate fed to weanling pigs

Two experiments were conducted to determine DE and ME, the apparent total tract digestibility (ATTD) of P, and the standardized total tract digestibility (STTD) of P in whey powder (3,646 kcal/kg), whey permeate (3,426 kcal/kg), and low-ash whey permeate (3,657 kcal/kg) fed to weanling pigs. The DE and ME in the 3 whey products were determined using 32 barrows (9.2 ± 0.4 kg of BW). A basal diet based on corn, soybean meal, and fish meal and 3 diets containing 70% of the basal diet and 30% of each whey product were prepared. Each diet was fed to 8 pigs that were housed individually in metabolism cages. The total collection method was used for fecal and urine collections with 5-d adaptation and 5-d collection periods, and the difference procedure was used to calculate DE and ME in the 3 whey products. The concentrations of DE in whey powder and low-ash whey permeate were greater (P < 0.001) than in whey permeate (3,646 and 3,683 vs. 3,253 kcal/kg of DM). The concentrations of ME in whey powder and low-ash whey permeate were also greater (P < 0.001) than in whey permeate (3,462 and 3,593 vs. 3,081 kcal/kg of DM). The ATTD and STTD of P in the 3 whey products were determined using 32 barrows (11.0 ± 0.81 kg of BW). Three cornstarch-sucrose-based diets containing 30% of each whey product as the sole source of P were prepared. A P-free diet that was used to estimate the basal endogenous losses of P was also formulated. The ATTD of P in whey powder and in whey permeate was greater (P < 0.001) than in low-ash whey permeate (84.3 and 86.1 vs. 55.9%), but the STTD values for P were not different among the 3 ingredients (91.2, 93.1, and 91.8% in whey powder, whey permeate, and low-ash whey permeate, respectively). In conclusion, whey permeate contains less GE, DE, and ME than whey powder and low-ash whey permeate, but all 3 ingredients have an excellent digestibility of P.     

Digestibility of nitrogen and amino acids in soybean meal with added soyhulls

This study was designed to quantify the effect of soyhulls on N and AA digestibilities of soybean meal for growing pigs. Soyhulls were incorporated into 17% CP diets containing 33.25% soybean meal (SBM) at 0, 3, 6, or 9% (as-fed basis) and fed to 35-kg barrows to determine their effect on apparent and true digestibility of DM, GE, N, and AA measured at the terminal ileum. Positive and negative control diets containing 1.05% lysine were formulated with 35% SBM and 27% soy protein concentrate (SPC), respectively. A low-protein, casein-based diet was used to estimate endogenous AA losses. Soyhulls were incorporated into experimental diets at the expense of cornstarch, and SBM levels were adjusted to accommodate the contribution of CP from soyhulls. Fourteen pigs were surgically fitted with simple T-cannula at the distal ileum and fed the seven semipurified cornstarch diets based on a replicated 7 x 7 Latin square design. Each period lasted 7 d, with diet acclimation from d 1 to 5 and ileal sample collection for 12 h on d 6 and 7. Feed was offered at a level of 90 g/kg BW(0.75) in two equal portions at 0800 and 2000. Apparent ileal digestibilities of DM and GE decreased approximately six percentage units with the addition of soyhulls (linear, P < 0.05), whereas N was not affected. Both apparent and true ileal digestibilities of arginine, histidine, lysine, phenylalanine, aspartic acid, serine, and tyrosine also exhibited a decrease (linear, P < 0.05) of up to five percentage units with the addition of soyhulls. True ileal lysine digestibility of SBM decreased from 90.3 to 87.7% with the addition of 9% soyhulls. The endogenous nutrient fraction measured at the distal ileum was rich in proline, glutamic acid, and glycine, with losses greater than 1,000 mg/kg of DMI for each AA, and contained minimal amounts of tryptophan, methionine, and cystine. The current data suggest that a 0.2% decrease in some true ileal indispensable AA digestibilities may result with each 1% increase in soyhull inclusion in semipurified diets containing SBM as the sole source of AA as fed to growing pigs.