日粮中高剂量植酸酶对肉鸡生长性能、骨骼矿化和钙磷沉积的影响

试验旨在研究含25-羟基维生素D3(25-OH-D3)低磷日粮中添加高剂量植酸酶对1~42日龄肉鸡生长性能、骨骼矿化和钙磷沉积率的影响。将250只1日龄肉鸡公雏随机分成5个处理,每个处理5个重复,每重复10只。1个对照组:非植酸磷(NPP)含量为0.45%/0.35%;4个试验组按照2×2因子设计试验:2个NPP含量(1~21日龄,NPP含量分别为0.35%和0.25%;22~42日龄,NPP含量分别为0.25%和0.15%),植酸酶2个添加水平为1 000 IU/kg和10 000 IU/kg。结果显示:(1)与日粮中NPP含量0.35%/0.25%相比,NPP含量为0.25%/0.15%时,42日龄肉鸡体增重、血浆无机磷浓度、股骨和胫骨灰分重量、钙含量以及跖骨直径和磷含量显著降低(P<0.05),31~34日龄肉鸡钙沉积率和11~14日龄肉鸡磷沉积率显著提高(P<0.05)。(2)与日粮中植酸酶添加1 000 IU/kg相比,植酸酶添加10 000 IU/kg时,42日龄肉鸡体增重、采食量、血浆无机磷浓度、股骨重量和灰分重量、胫骨和跖骨长度显著提高(P<0.05),11~14日龄肉鸡钙磷沉积率显著提高(P<0.05)。(3)与日粮中NPP含量0.45%/0.35%相比,日粮中0.35%/0.25%NPP+1 000 IU/kg植酸酶或0.25%/0.15%NPP+10 000 IU/kg植酸酶时,肉鸡生长性能和骨骼矿化(除跖骨磷含量外)未受到显著影响(P>0.05)。综上所述,1~42日龄肉鸡日粮中NPP含量为0.35%/0.25%时,植酸酶适宜添加量为1 000 IU/kg;NPP含量为0.25%/0.15%时,植酸酶适宜添加量为10 000 IU/kg。     

葡萄糖酸钠和植酸酶对肉仔鸡磷利用的影响

选取280只3周龄的从雄性商品内鸡进行试验,研究玉米一豆粕型低磷饲粮中添加葡萄糖酸钠和植酸酶对内鸡磷利用的影响.试验采用2×3+1设计,其中葡萄糖酸钠两个水平(0,2%),植酸酶3个水平(0,500,1000 U/kg).另设一满足NKC(1994)钙、磷需要的正常饲粮,共构成7个处理,每个处理5个重复,每个重复8只鸡.试验期为3-6周龄.自由采食和饮水.结果表明:与正对照组相比.低磷基础饲粮显著降低了肉仔鸡的胫骨灰分含量(P<0.001);添加2%葡萄糖酸钠显著提高了肉仔鸡的血清钙水平(P=0.035);添加植酸酶提高了肉仔鸡的钙(P=0.020)、磷表观存留率(P＝0.036)和胫骨灰分含量(P<0.001).未观察到葡萄糖酸钠和植酸酶之间存在互作(P>0.05).这些结果说明,葡萄糖酸钠和植酸酶均可改善后期肉鸡对磷的利用.     

不同能量和有效磷水平下植酸酶对肉鸡能量和养分利用率的影响

试验选用640只1日龄AA肉公鸡,采用2×2×2因子设计,通过在不同能量水平(11.90或11.70MJ/kg)和有效磷水平(0.35%或0.25%)的肉鸡饲粮中添加植酸酶、0500FTU/kg,考察了植酸酶对肉鸡养分利用率的影响。试验共8个处理,每个处理8个重复,每个重复10只鸡,试验期42d。结果表明:饲粮添加植酸酶提高了干物质、粗蛋白、钙、磷的利用率,分别提高0.97%(P<0.10)、1.49%(P<0.20)、8.55%(P<0.01)、8.61%(P<0.01);也提高了必需氨基酸、非必需氨基酸、总氨基酸(P<0.20),苏氨酸、甘氨酸、丙氨酸、缬氨酸(P<0.10)和异亮氨酸(P<0.05)的表观回肠利用率,但对能量利用率没有显著影响(P>0.05)。植酸酶和能量对干物质、总能、磷的利用率存在极显著的交互作用(P<0.01),表现为对照加酶组干物质、总能利用率极显著高于低能组,低能加酶组磷的利用率极显著高于低能组。这表明饲粮添加植酸酶可以提高肉鸡的养分利用率,低营养水平饲粮添加植酸酶作用效果更好。     

低钙磷饲粮添加高剂量植酸酶对1～63日龄黄羽肉鸡生长性能及胫骨性能的影响

试验旨在研究低钙磷饲粮添加高剂量植酸酶对1～63日龄黄羽肉鸡生长性能及胫骨性能的影响。选用1 080只1日龄岭南黄羽肉公雏,根据体重一致原则分为6个处理,每个处理6个重复,每个重复30只。试验期63 d。结果表明:①植酸酶的添加可显著提高体重、平均日增重(P<0.05);②添加植酸酶显著提高了胫骨骨密度(P<0.05);MP+4 000、LP+4 000组胫骨脱水脱脂重显著提高(P<0.05);MP+4 000组胫骨灰分含量、磷含量显著提高(P<0.05);植酸酶的添加对胫骨钙含量无显著影响(P>0.05)。综上所述,低钙磷饲粮添加高剂量植酸酶可提高1～63日龄黄羽肉鸡生长性能,改善胫骨性能。可考虑用4 000 FTU/kg植酸酶部分替代或者完全替代玉米-豆粕型饲粮中磷酸氢钙。     

玉米-豆粕型日粮中添加高剂量植酸酶对肉鸡养分利用率的影响

本试验研究了玉米-豆粕型日粮中添加高剂量不同水平的植酸酶对0~3和4~6周肉鸡养分利用率的影响。选用1日龄AA肉鸡公雏576只,随机分为6组,每组8个重复,每个重复12只。试验日粮分0~3和4~6周两个阶段,6个处理组:正对照组(前期为总磷0.69%,0.45% NPP;后期为总磷0.59%,0.35% NPP);负对照组(前期总磷0.60%,0.35% NPP;后期为总磷0.49%,0.25% NPP);4个试验组在负对照日粮中分别添加500、2000、8000和32000 FTU/kg 4种植酸酶水平。结果显示:①日粮添加高剂量不同水平的植酸酶显著影响生长前期(17~21日龄)肉鸡的钙、磷的消化利用率(P<0.05),随着植酸酶添加水平增加,钙的消化利用率显著增加(P＜0.05)。②日粮添加高剂量不同水平的植酸酶显著影响38~42日龄肉鸡磷的消化利用率(P＜0.05)。在负对照组日粮中随着植酸酶水平添加的提高,钙的消化利用率呈现先增加后降低的趋势,磷的养分利用率反而显著减少。③日粮添加高剂量不同水平植酸酶显著增加生长前期(17~21日龄)肉鸡的粗蛋白质的消化利用率和表观代谢能(P＜0.05),在生长后期(38~42日龄)对肉鸡的粗蛋白质的消化利用率和表观代谢能提高达到显著水平(P ＜0.05)。因此,高剂量不同水平的植酸酶显著提高肉鸡AME和粗蛋白质、钙、磷的消化利用率,添加高水平植酸酶可以适当调低日粮能量和蛋白质水平,日粮中总磷(tP)水平应低于NRC标准,减少无机磷和钙的添加量,以提高钙、磷在体内的存留量。     

微生物植酸酶与1～21日龄肉鸡饲粮无机磷当量模型研究

本试验研究饲粮非植酸磷（NPP）和植酸酶添加水平对1～21日龄肉鸡生长性能、养分利用、血液指标及胫骨发育的影响,确定微生物植酸酶与无机磷当量关系。试验设计10个处理,每个处理4个重复,每个重复10只鸡。前4种饲粮NPP水平为2.1、2.9、3.7和4.5 g/kg;后6种饲粮在基础饲粮（NPP 2.1 g/kg,总磷（tP）4.5 g/kg）中添加植酸酶250、500、1 000、2 000、4 000和8 000 U/kg。结果显示：（1）随饲粮NPP水平升高,肉鸡体增重、采食量、饲料转化率、血浆无机磷浓度、胫骨长度、强度、灰分含量与重量、胫骨磷含量以及粗蛋白质、tP和Ca利用率呈二次曲线升高,而死亡率、血浆Ca含量及碱性磷酸酶活性呈二次曲线降低;（2）随植酸酶水平升高,肉鸡生长性能、血浆和胫骨指标、养分利用率呈现与NPP水平增加类似的趋势;（3）当植酸酶水平达到8 000 U/kg时,肉鸡生长性能、血浆和胫骨指标以及粗蛋白质利用率达到对照组（NPP 4.5 g/kg）水平,总磷利用率提高到85%,植酸磷释放率达到94.84%;（4）以胫骨灰分重量为指标,饲粮中无机磷释放量（y,g/kg）与微生物植酸酶添加水平（x,U/kg）之间的关系模型为y = 0.425 4 &#215; log2（1 ＋ x/250） ＋ 0.283 （n=7,R^2 = 0.915 3,P=0.000 7）。结果表明,高水平植酸酶可降解肉鸡饲粮中几乎全部植酸磷,植酸酶添加水平与无机磷当量模型可为确定配方中磷水平提供参考。     

植酸酶对肉鸡日粮养分表观利用率和表观代谢能的影响

试验在肉鸡玉米-豆粕型日粮中添加500U/kg植酸酶,研究植酸酶对肉鸡生长性能、养分利用率和骨骼指标的影响。选择1日龄AA肉仔鸡384只(公母鸡各192只)。按体重相近,性别各半的原则,随机分成4组,每组设6个重复,每个重复16只。Ⅰ组为不添加植酸酶的基础日粮组(对照组);Ⅱ、Ⅲ、Ⅳ组为日粮中添加500U/kg植酸酶分别替代0.09%、0.11%、0.13%的无机磷。试验分为试验前期(0～3周)和后期(4～6周)。结果表明:在肉鸡玉米-豆粕型日粮中添加植酸酶可提高日粮干物质、有机物、粗蛋白的利用率,但差异不显著(P>0.05);钙利用率提高了17.23%(前期)和22.86%(后期),差异达到显著水平(P<0.05);磷利用率提高了18.98%(前期)和20.73%(后期),差异显著(P<0.05)。肉鸡对饲料的能量利用率提高了1.94%(前期)和1.63%(后期),差异显著(P<0.05)。     

不同剂型植酸酶对蛋鸡产蛋性能、蛋品质和骨骼质量的影响

本研究旨在探讨2种剂型(粉状和颗粒状)植酸酶对产蛋高峰期蛋鸡产蛋性能、蛋品质和骨骼质量的影响.选取1 440只30周龄产蛋高峰期罗曼粉壳蛋鸡随机分为4个处理,每个处理10个重复,每个重复36只.处理Ⅰ为正对照组,饲喂基础饲粮,处理Ⅱ为负对照组,在试验期的第1～5周在正对照组饲粮的基础上分别降低钙和有效磷水平至3.23%和0.24%,而在试验期的第6～10周则降至3.23%和0.19%.处理Ⅲ在处理Ⅱ的基础上添加粉状植酸酶(300 U/kg),处理Ⅳ在处理Ⅱ的基础上添加颗粒状植酸酶(300 U/kg).试验期10周.结果表明,低磷饲粮显著降低全期平均日采食量和胫骨灰分含量(P<0.05),提高后期破蛋率(P<0.05),对日产蛋率、平均蛋重、蛋品质等无显著影响(P>0.05).添加2种植酸酶均显著提高平均日采食量(P<0.05)和胫骨灰分含量(P<0.01),但2种植酸酶之间产蛋率、平均蛋重、蛋品质等无显著差异(P>0.05).添加粉状植酸酶能显著提高灰分中磷的含量(P<0.05),添加颗粒状植酸酶显著降低了后期破蛋率(P<0.05).由此可知,在低磷蛋鸡饲粮中添加植酸酶可显著提高蛋鸡平均日采食量和胫骨灰分含量,对蛋品质的影响不显著.2种剂型的植酸酶对产蛋性能、蛋品质和骨骼质量的影响无显著差异.     

肉鸡饲粮能量水平对植酸酶作用效果的影响

试验选用324只1日龄AA肉鸡(公鸡),采用3×2因子设计,研究在不同能量水平的饲粮中添加植酸酶对肉鸡生长性能和养分利用率的影响。试验设3个能量水平(12.33、12.13、12.03MJ/kg)和2个酶水平(0、500FTU/kg),共计6个处理组,每个处理组6个重复,每个重复9只鸡,试验期42d。结果表明:饲粮添加植酸酶显著(P<0.05)或极显著(P<0.01)提高了肉鸡日增重、采食量,改善了饲料利用率,提高了干物质、粗蛋白、钙、磷的利用率,但对能量利用率没有明显影响(P>0.05);降低饲粮能量水平极显著降低了肉鸡对干物质、能量、钙的利用率(P<0.01),显著降低了其对磷的利用率(P<0.05),但对生产性能和粗蛋白利用率没有显著影响(P>0.05);植酸酶和能量对肉鸡饲料利用率、干物质和钙的利用率存在显著的交互作用(P<0.05),表现为处理2组能量水平条件下添加植酸酶在生长性能和养分利用率上改善幅度最大,表明植酸酶在适宜降低能量水平的饲粮中的作用效果更好。试验中,以能量水平为12.13MJ/kg的肉公鸡饲粮中添加500FTU/kg植酸酶(处理5组)为最佳组合。     

玉米-豆粕型日粮中添加高剂量植酸酶对 肉鸡生长性能和血清生化指标的影响

本试验研究了玉米—豆粕型日粮中添加高剂量不同水平的植酸酶对0~3和3~6周肉鸡生长性能和血清生化指标的影响。选用1日龄AA肉公雏576只,随机分为6组,每组8个重复,每个重复12只。试验日粮分0~3和3~6周两个阶段,6个处理组分别为:正对照组(前期总磷0.69%,0.45% NPP;后期总磷0.59%,0.35% NPP);负对照组(前期总磷0.60%,0.35% NPP;后期总磷0.49%,0.25% NPP);4个试验组在负对照日粮中分别添加500、2000、8000和32000 FTU/kg 4种水平的植酸酶。结果显示,①日粮添加植酸酶水平显著影响前期(1~21日龄)平均日增重(P＜0.05),对采食量和饲料转化效率(F∶G)影响差异不显著(P＞0.05)。②日粮添加植酸酶水平对后期(22~42日龄)平均日增重和饲料转化效率(F∶G)影响差异显著(P＜0.05),添加植酸酶水平对采食量影响差异不显著(P＞0.05)。③在低磷日粮中添加高水平植酸酶8000 FTU/kg时,饲料转化效率最高。④随着植酸酶水平增加,21日龄肉鸡血清钙水平有降低趋势,血清磷水平显著增加(P＜0.05),血清碱性磷酸酶活性显著降低(P＜0.05),对总蛋白影响差异不显著(P＞0.05)。植酸酶添加水平影响42日龄肉鸡血清钙水平、总蛋白水平和血清碱性磷酸酶活性,对血清磷水平影响显著(P＜0.05)。因此,在低磷日粮中添加高剂量不同水平的植酸酶肉鸡的生长性显著增加,碱性磷酸酶的活性显著降低,添加8000 FTU/kg效果优于500、2000和32000 FTU/kg。     

饲粮类型和肥育后期不添加维生素和微量矿物元素对猪生长性能、胴体和肌肉品质、粪中矿物元素排泄的影响

本研究共开展两个试验,探讨饲粮类型和不添加维生素和微量矿物元素对猪肥育后期生长性能、胴体和肌肉品质、粪中微量矿物元素排泄的影响。在试验1中,选用128头平均体重(78.5±4.6)kg的肥育猪,根据体重和性别分成4组,每组4圈(重复),每个重复8头猪。四组试验猪的试验处理为2×2因子设计,即两种类型(玉米-豆粕型和玉米-杂粕型)饲粮和添加或不添加维生素/微量矿物元素预混料。在试验2中,选用112头平均体重(90.3±6.3)kg的肥育猪,根据体重和性别分成4组,每组4圈(重复),每个重复7头猪。试验处理同试验1。结果显示,在79~110kg肥育期中(试验1),采食玉米-豆粕型饲粮的猪的增重速度和采食量显著高于采食玉米-杂粕型饲粮的猪(P<0.01或0.05)。在90~105kg肥育期中(试验2),采食玉米-豆粕型饲粮的猪的增重速度仍然高于采食玉米-杂粕型饲粮的猪(P<0.05)。但是,维生素和微量矿物元素添加与否对生长性能无显著影响(P>0.05)。饲粮类型和不添加维生素和微量矿物元素对胴体和肌肉品质均无显著影响(P>0.05)。粪中微量矿物元素含量不受饲粮类型的影响(P>0.05),但不添加维生素和微量矿物元素时,粪中铜、铁、锰的含量显著降低(P<0.01),粪中锌含量也有降低的趋势(P>0.05)。对于生长性能、胴体和肌肉品质以及微量矿物元素排泄量,饲粮类型×维生素/微量矿物元素预混料的交互作用不显著(P>0.05)。结果表明,在猪的肥育后期(最后约25~40d),在玉米-豆粕型和玉米-杂粕型饲粮中可不添加维生素和微量矿物元素,从而可降低饲料成本和减少微量矿物元素的排泄。     

不同清洁型日粮降低规模猪场中氮、磷污染物排泄的研究

本试验旨在研究在肥育猪日粮中添加植酸酶、复合酶及微生态制剂3种清洁型日粮对规模猪场氮、磷排泄的影响。试验选取健康无病的60 kg左右的苏钟猪15头,随机分为5个处理,分别为对照组(正常磷水平)、负对照组(不加CaHPO₄)、负对照+植酸酶组、对照+植酸酶+复合酶+和美酵素组、对照+微生态制剂组。结果表明,3种清洁型日粮均可降低粪、尿中氮的排泄量,其中植酸酶组粪中氮的排泄量较对照组降低了0.55%(P＞0.05),植酸酶+复合酶+和美酵素组降低了10%(P＜0.05),微生态制剂组降低了6.11%(P＞0.05),其中植酸酶+复合酶+和美酵素组减排效果更佳,尿氮含量各组之间差异不显著(P＞0.05);粪中磷的排泄量植酸酶组较对照组降低了19.19%(P＞0.05),植酸酶+复合酶+和美酵素组降低了36.63%(P＜0.05),微生态制剂组降低了16.28%(P＞0.05),其中植酸酶+复合酶+和美酵素组减排效果最佳,尿磷的排泄量植酸酶组降低了12%(P＞0.05),植酸酶+复合酶+和美酵素组降低了16%(P＜0.05),微生态制剂组降低了12%(P＞0.05),以植酸酶+复合酶+和美酵素组减排效果最好。由此表明,在肥育猪日粮中添加清洁饲料可有效降低规模猪场中氮、磷排泄造成的环境污染,且以植酸酶、复合酶及和美酵素联合应用效果最佳。     

蒸汽压片玉米对奶牛生产性能和氮磷排放影响的研究

本试验旨在研究蒸汽压片玉米对泌乳早期中国荷斯坦奶牛生产性能和氮磷排放的影响.试验采用4×4重复拉丁方设计.将8头泌乳早期中国荷斯坦奶牛随机分为4组,每组2头.4种日粮处理中蒸汽压片玉米含量分别为精料的0、10%、20%和30%.试验分4期,每期28 d.结果表明:日粮中添加蒸汽压片玉米对奶牛干物质采食量无显著影响(P＞0.05),但显著提高了饲料转化率(奶料比)(P＜0.05);显著提高了奶牛产奶量,其中20%与30%组的4%标准奶产量(FCM)产量分别比对照组提高2.78和2.87 kg·d~(-1)(P＜0.01).对乳成分无显著影响(P＞0.05);乳蛋白、乳糖、乳非脂固形物含量呈增加趋势;对营养物质的表观消化率无显著影响(P＞0.05);日粮中添加蒸汽压片玉米显著降低了乳尿素氮水平(P＜0.05).试验组奶牛氮排放量分别比对照组降低了4.23%(P＞0.05)、9.37%(P＜0.05)和10.29%(P＜0.05),磷排放量分别比对照组降低了1.93%(P＞0.05)、2.59%(P＞0.05)和5.19%(P＞0.05).因此,饲喂蒸汽压片玉米能够有效提高奶牛的生产性能,降低氮磷的排放.     

Effects of Source of Inorganic Phosphorus and Phytase Supplementation on Performance, Physiological Parameters and Bone Mineralization in Broilers

The objective of this study was to eval- uate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1,150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phos- phorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2,120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemen- ted with 0,250,500 ,or 1,000 P-＇rU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conver- sion as a result of feeding the different sources of in- organic phosphorus. The digestibility of phosphorus was significantly lower （P =0.01 ） for chicks fed di- ets supplemented with tricalcium phosphate than for chicks fed the other two diets. However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feedconversion showed a linear improvement （P = 0.03 ） with increasing levels of phytase inclusion （ days 0 to 33 ）. Phytase supplementation resulted in linear increa- ses in the digestibility of dry matter （P = 0.02 ）, crude protein （ P --- 0.04 ） and energy （ P 〈 0.01 ）. Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium （ P = 0.05 ） and bone breaking strength （P = 0.04 ） than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets sup- plemented with dicalcium phosphate, tricalcium phos- phate or defluorinated phosphate was similar and therefore production costs could be lowered by choo- sing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formu- lated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase sup- plementation. However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be suf- ficient justification for the routine inclusion of phytase in diets fed to broilers.     

Effects of a New Recombinant Phytase on the Performance and Mineral Utilization of Broilers Fed Phosphorus-Deficient Diets

An experiment was conducted with Arbor Acres broiler chickens that were fed 3 experimental diets—a control diet containing an adequate level of available phosphorus (AP) and 2 diets that were deficient in AP but supplemented with phytase at a level of either 500 or 750 phytase units/kg—to assess the effects of a novel microbial phytase supplement in broilers fed AP-deficient diets on growth performance and mineral utilization. Similar average daily gain, feed intake, and feed efficiency (P > 0.05) were obtained among broilers fed different diets. Compared with broilers fed the control diet, broilers fed diets with phytase had greater (P < 0.05) retention of Ca, P, and Zn. Moreover, the levels of Cu, Zn, Mg, and Mn in the tibia bone at 28 d of age, and Zn and Mn at 42 d of age in birds fed diets with phytase exceeded (P < 0.05) those of birds fed the control diet. Supplementation of phytase increased Zn and Mg contents in the plasma at 42 d of age. Birds responded similarly to phytase supplemented at a level of 500 or 750 phytase units/kg in terms of growth performance, mineral retention, and mineral content in the serum and bone. Therefore, with the supplementation of this novel phytase, it is possible to reduce the dietary levels of P and other minerals to below the recommended levels of the Feeding Standard of Chicken in P. R. China (ZB B 43005-86).     

Dietary cholecalciferol and phosphorus influence intestinal mucosa phytase activity in broiler chicks

1. The role of cholecalciferol and phosphorus in the regulation of intestinal mucosa phytase was investigated in broiler chicks. 2. A total of 144 7-d-old male broiler chicks were grouped by weight into 6 blocks of 4 cages with 6 broiler chicks per cage. Four maize-soybean meal-based mash diets were randomly assigned to cages within each block. The 4 diets consisted of cholecalciferol at 0 or 75 microg/kg and total phosphorus at 3.6 or 7.0 g/kg in a 2 x 2 factorial arrangement. The birds were given the experimental diets for 12 d under conditions which excluded ultraviolet light. 3. Broiler chicks fed on diets with the higher concentration of cholecalciferol had higher Vmax and Km of the mucosa phytase, weight gain, feed intake, feed efficiency and percentage tibia ash, higher ileal digestibility of dry matter, energy, phosphorus (P) and calcium (Ca), and increased retention of dry matter, nitrogen, P, Ca and energy. 4. Broiler chicks receiving diets with the higher P concentration showed lower Vmax and Km of the intestinal mucosa phytase but greater weight gain, feed intake, feed efficiency and percentage tibia ash, higher ileal digestibility of dry matter, energy, P and nitrogen, and increased retention of dry matter, energy, nitrogen and Ca. 5. In conclusion, both dietary P and cholecalciferol influenced the activity of intestinal mucosa phytase.     

Effects of individual or combined xylanase and phytase supplementation on energy, amino acid, and phosphorus digestibility and growth performance of grower pigs fed wheat-based diets containing wheat millrun

The objective of these studies was to determine if dietary enzymes increase the digestibility of nutrients bound by nonstarch polysaccharides, such as arabinoxylans, or phytate in wheat millrun. Effects of millrun inclusion rates (20 or 40%), xylanase (0 or 4,375 units/kg of feed), and phytase (0 or 500 phytase units/kg of feed) on nutrient digestibility and growth performance were investigated in a 2 x 2 x 2 factorial arrangement with a wheat control diet (0% millrun). Diets were formulated to contain 3.34 Mcal of DE/kg and 3.0 g of true ileal digestible Lys/Mcal of DE and contained 0.4% chromic oxide. Each of 18 cannulated pigs (36.2 +/- 1.9 kg of BW) was fed 3 diets at 3x maintenance in successive 10-d periods for 6 observations per diet. Feces and ileal digesta were collected for 2 d. Ileal energy digestibility was reduced (P < 0.01) linearly by millrun and increased by xylanase (P < 0.01) and phytase (P < 0.05). Total tract energy digestibility was reduced linearly by millrun (P < 0.01) and increased by xylanase (P < 0.01). For 20% millrun, xylanase plus phytase improved DE content from 3.53 to 3.69 Mcal/kg of DM, a similar content to that of the wheat control diet (3.72 Mcal/kg of DM). Millrun linearly reduced (P < 0.01) ileal digestibility of Lys, Thr, Met, Ile, and Val. Xylanase improved (P < 0.05) ileal digestibility of Ile. Phytase improved ileal digestibility of Lys, Thr, Ile, and Val (P < 0.05). Millrun linearly reduced (P < 0.05) total tract P and Ca digestibility and retention. Phytase (P < 0.01) and xylanase (P < 0.05) improved total tract P digestibility, and phytase and xylanase tended to improve (P < 0.10) P retention. Phytase improved Ca digestibility (P < 0.05) and retention (P < 0.01). The 9 diets were also fed for 35 d to 8 individually housed pigs (36.2 +/- 3.4 kg of BW) per diet. Millrun reduced (P < 0.05) ADFI, ADG, and final BW. Xylanase increased (P < 0.05) G:F; phytase reduced (P < 0.05) ADFI; and xylanase tended to reduce (P = 0.07) ADFI. In summary, millrun reduced energy, AA, P, and Ca digestibility and growth performance compared with the wheat control diet. Xylanase and phytase improved energy, AA, and P digestibility, indicating that nonstarch polysaccharides and phytate limit nutrient digestibility in wheat byproducts. The improvement by xylanase of energy digestibility coincided with improved G:F but did not translate into improved ADG.     

Effects of supplemental dietary phytase and pharmacological concentrations of zinc on growth performance and tissue zinc concentrations of weanling pigs

Three experiments were conducted to determine the effects of phytase, excess Zn, or their combination in diets for nursery pigs. In all experiments, treatments were replicated with five to seven pens of six to seven pigs per pen, dietary Ca and available P (aP) levels were decreased by 0.1% when phytase was added to the diets, excess Zn was added as ZnO, a basal level of 127 mg/kg of Zn (Zn sulfate) was present in all diets, and the experimental periods were 19 to 21 d. In Exp. 1, pigs (5.7 kg and 18 d of age) were fed two levels of phytase (0 or 500 phytase units/kg) and three levels of excess Zn (0, 1,000, or 2,000 ppm) in a 2 x 3 factorial arrangement. Added Zn linearly increased ADG and ADFI during Phase 1 (P = 0.01 to 0.06), Phase 2 (P = 0.02 to 0.09), and overall (P = 0.01 to 0.02). Gain:feed was linearly increased by Zn during Phase 1 (P = 0.01) but not at other times. Dietary phytase decreased ADG in pigs fed 1,000 or 2,000 ppm Zn during Phase 2 (Zn linear x phytase interaction; P = 0.10), did not affect (P = 0.27 to 0.62) ADFI during any period, and decreased G:F during Phase 2 (P = 0.01) and for the overall (P = 0.07) period. Plasma Zn was increased by supplemental Zn (Zn quadratic, P = 0.01) but not affected (P = 0.70) by phytase addition. In Exp. 2, pigs (5.2 kg and 18 d of age) were fed two levels of phytase (0 or 500 phytase units/kg) and two levels of Zn (0 or 2,000 ppm) in a 2 x 2 factorial arrangement. Supplemental Zn increased ADG and G:F during Phase 2 (P = 0.02 to 0.09) and overall (P = 0.07 to 0.08), but it had no effect (P = 0.11 to 0.89) on ADG during Phase 1 or ADFI during any period. Phytase supplementation increased ADG (P = 0.06) and G:F (P = 0.01) during Phase 2. Gain:feed was greatest for pigs fed 2,000 ppm Zn and phytase (Zn x phytase interaction; P = 0.01). Bone (d 20) and plasma Zn (d 7 and 20) were increased (P = 0.01) by added Zn but not affected (P = 0.51 to 0.90) by phytase. In Exp. 3, pigs (5.7 kg and 19 d of age) were fed a basal diet or the basal diet with Ca and aP levels decreased by 0.10% and these two diets with or without 500 phytase units/kg. Supplemental phytase had no effect (P = 0.21 to 0.81) on growth performance. Reduction of dietary Ca and aP decreased (P = 0.02 to 0.08) ADG, ADFI, and G:F for the overall data. These results indicate that excess dietary supplemental Zn increases ADG and plasma and bone Zn concentrations. Dietary phytase did not affect plasma or bone Zn concentrations.     

Effects of Source of Inorganic Phosphorus and Phytase Supplementation on Performance, Physiological Parameters and Bone Mineralization in Broilers

The objective of this study was to evaluate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1, 150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phosphorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2, 120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemented with 0, 250, 500, or 1,000 FTU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conversion as a result of feeding the different sources of inorganic phosphorus. The digestibility of phosphorus was significantly lower (P = 0.01) for chicks fed diets supplemented with tricalcium phosphate than for chicks fed the other two diets.  However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feed conversion showed a linear improvement (P = 0.03) with increasing levels of phytase inclusion (days 0 to 33).  Phytase supplementation resulted in linear increases in the digestibility of dry matter (P = 0.02), crude protein (P = 0.04) and energy (P < 0.01).  Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium (P = 0.05) and bone breaking strength (P = 0.04) than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets supplemented with dicalcium phosphate, tricalcium phosphate or defluorinated phosphate was similar and therefore production costs could be lowered by choosing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formulated to meet dietary phosphorus requirements, the growth of broilers was not enhanced with phytase supplementation.  However, increases in feed conversion and bone breaking strength and its potential to impact culling and mortality in broiler operations may be sufficient justification for the routine inclusion of phytase in diets fed to broilers.