Epizootic diarrhea of infant mice: indentification of the etiologic agent

Electron micrographs of intestinal epithelium of infant mice infected with epizootic diarrhea virus have demonstrated intracellular spherical structures measuring 65 to 75 m(micro) in diameter which have a complex morphology resembling several virus particles. They have ben interpreted as being the etiologic agent of this disease. The particles were present in association with, in some cases within, vesicles of the endoplasmic reticulum of intestinal epithelial cells. They were never seen in the nucleus.     

烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析

采用PCR-RFLP方法对烟台黑猪DQB基因外显子2的多态性进行研究,并对该位点与仔猪腹泻的关联进行了分析。结果表明,在烟台黑猪DQB基因外显子2上共检测出4种等位基因(A、B、C、D),7种RFLP带型(AA、BB、CC、AB、AC、BC、AD),该位点具有较丰富的多态性。卡方适合性检验显示,烟台黑猪的DQB基因外显子2没有达到Hardy-Weinberg平衡(P<0.05)。最小二乘法分析结果表明,烟台黑猪的性别和DQB外显子2的基因型均对仔猪腹泻无显著影响(P>0.05),而性别与基因型的互作效应对仔猪腹泻具有极显著的影响(P<0.01),在公猪群体中,AD基因型仔猪腹泻评分高于AA基因型个体(P<0.05),在母猪群体中BB、AC、BC、CC基因型仔猪腹泻评分高于其他基因型仔猪(P<0.05)。     

Fish: serologic evidence of infection with human pathogens

Specific antibodies to several bacteria pathogenic to humans were detected in the serums of white perch from surface waters adjacent to heavily populated areas on Chesapeake Bay. White perch from surface waters adjacent to sparsely populated areas were free of such antibodies. We suggest that fish may become actively infected with human pathogens by exposure to contaminated water and may constitute a hazard to public health.     

难辨梭状芽孢杆菌相关性腹泻与抗生素相关性腹泻的临床流行病学比较

目的对比难辨梭状芽孢杆菌相关性腹泻（CDAD）与抗生素相关性腹泻（AAD）的临床特点,分析CDAD的危险因素。方法对2008年4至11月诊断为抗生素相关性腹泻（AAD）的202例住院患者,用酶联荧光免疫检测粪便中难辨梭状芽孢杆菌毒素A/B,前瞻性对比分析CDAD和AAD的临床特点。结果202例AAD中有44例CDAD（占21.8%）。多因素分析显示：高APACHE Ⅱ评分、合并慢性病、高血C反应蛋白及低血白蛋白水平是引起CDAD的危险因素（P＜0.05）,而使用甲硝唑或万古霉素是CDAD的保护性因素。结论AAD中约21%由难辨梭状芽孢杆菌感染所致,病情危重、慢性疾病史、免疫功能低下及抗生素应用是引起CDAD的危险因素。     

中山市农民专业协会发展的经验与思路

介绍了中山市农民专业协会的概况和特点,分析了中山市政府促进和扶持农民专业协会的主要做法和经验,并探讨了中山市农民专业协会下一步的发展思路.     

K88菌毛及其在仔猪断奶腹泻预防制剂开发中的应用

K88大肠杆菌是引起仔猪断奶后腹泻(PWD)的主要病原.现有商业疫苗通过母猪免疫能有效控制新生仔猪大肠杆菌性腹泻,而对PWD却难以奏效.口服疫苗能诱导肠粘膜产生局部抗体,阻止致病性大肠杆菌的粘附,进而预防PWD.从分子水平认识K88菌毛及其受体的生物学特性,对口服疫苗的研制具有重要意义.文章概述国内外关于K88菌毛基本特性、蛋白亚基及受体的研究,就K88菌毛在PWD预防制剂研发中的应用的进展作了综述,并探讨分析了口服基因工程细菌疫苗和植物疫苗的前景.     

以农会为载体促进农村经济发展

在正式加入世贸组织后,我国农业如何应对WTO环境?怎样才能做好"三农"工作?如何看待农村人口迁移?小城镇建设抓什么?农业产业结构该怎么调整?这些都成了热门话题.基层党委政府认为,目前主抓调整好产业结构以增加农民收入.     

Splice junctions: association with variation in protein structure

A comparison between eukaryotic gene sequences and protein sequences of homologous enzymes from bacterial and mammalian organisms shows that intron-exon junctions frequently coincide with variable surface loops of the protein structures. The altered surface structures can account for functional differences among the members of a family. Sliding of the intron-exon junctions may constitute one mechanism for generating length polymorphisms and divergent sequences found in protein families. Since intron-exon junctions map to protein surfaces, the alterations mediated by sliding of these junctions can be effected without disrupting the stability of the protein core.     

多聚免疫球蛋白受体(pIgR)基因多态性及其与仔猪腹泻关系

采用PCR-SSCP结合测序方法,在民猪、长白猪两群体中检测p Ig R基因外显子1多态性,研究母猪基因多态性与仔猪腹泻关系。结果表明,p Ig R基因外显子1存在两个错义突变,第373位C→T导致丙氨酸→缬氨酸变异,第394位A→G导致天冬氨酸→丝氨酸变异。民猪群体有AA、AB和BB三种基因型,长白猪群体有AA和AB两种基因型,两群体基因型分布差异极显著(P0.01)。民猪群体,不同基因型母猪仔猪腹泻指数和腹泻致死率均存在显著差异(P0.05),但长白猪群体,仅腹泻指数表现显著差异(P0.05)。     

恩诺沙星对肉鸡白痢的防治效果

用恩诺沙星等药物对肉鸡白痢作治疗试验，结果表明，恩诺沙星对鸡白痢的治愈率达90％。     

宁夏农村专业技术协会的发展现状与对策

农村专业技术协会已成为推动宁夏农业产业化和农村经济发展、促进农民增收、推进社会主义新农村建设的一支重要的社会力量.通过实际调研和调查,对宁夏农村专业技术协会的发展现状和存在的问题进行了分析,提出进一步完善和发展宁夏农村专业技术协会的对策,为此领域研究提供参考.     

Favism: association with erythrocyte acid phosphatase phenotype

The frequency of carriers of the P(a)and P(c) alleles of the gene for acid phosphatase in the erythrocyte is significantly higher in male subjects deficient in glucose-6-phosphate dehydrogenase and having hemolytic clinical favism than it is in the general population. This observation seems to indicate that alleles (P(a) and P(c)) of a gene polymorphic in all human populations affect the fitness of the involved phenotypes in special genotypic and nongenotypic conditions.     

基于代理程序快速开发生物信息二次数据库

以构建鸡基因组全序列二次数据库为例,探讨Windows操作系统下,编译Web代理程序,快速开发生物信息二次数据库的一般过程。研究通用代理程序开发本地化生物信息二次数据库的具体步骤,所涉及的关键技术以及常见问题解决。解决了海量生物信息自动下载与处理问题,经反复调试实现了示例程序鸡基因组全序列二级数据库系统的快速构建。     

仔兔腹泻病因分析及防治措施

近年来,不少养兔场和养兔专业户反映仔兔因腹泻而导致死亡率高、成活率低,严重影响了养兔的经济效益,造成一些农户养兔积极性不高.     

Airborne particulates in Pittsburgh: association with p, p -DDT

The DDT associated with samples of airborne particulates was measured by gas chromatography. Because of possible vaporization of DDT during sampling, concentrations reported represent minimum values but demonstrate that DDT can be present in an urban atmosphere that is distant from any large-scale agricultural activity.     

Ozone concentrations in new jersey and new york: statistical association with related variables

The concentrations of ozone at nine measurements sites in New Jersey and New York during the period 1 May through 30 September 1973 have been examined. Daily fluctuations in the ozone concentrations at any two sites are highly correlated. The concentrations are lower with low levels of solar radiation and also with high wind speed. The average ozone concentration shows only minor differences between weekdays and weekends, despite markedly different traffic patterns.     

犊牛腹泻的发病原因及治疗的研究进展

犊牛腹泻（calfdiarrhea）是危害犊牛常见的疾病之一,严重地影响着奶产业的健康发展,探究犊牛腹泻的发病病因及机理,对犊牛腹泻的防治及奶业健康可持续发展意义重大．本文从犊牛腹泻的发病原因、临床症状、中西医相结合治疗等方面的研究进展进行了综述．     

盐碱改良剂--禾康盐碱清除剂在棉地的使用效果

禾康盐碱清除剂是一种盐碱改良剂，它是通过有机高分子络合土壤中盐离子，再结合灌水，降盐降碱，并能消除盐分子引起的毒害和渗透压，改善土壤理化性质，增加水分和养分，提高作物抗旱能力。为了探讨禾康盐碱清除剂的效果．给大面积开发和改良盐碱地提供科学依据．我们做了如下试验。     

畜禽好得快饮水剂研制和应用报告

选用水溶性能好,不显示副作用,无蓄积的抗菌药E作为饮水剂的抗菌成分.抗菌药E与氯化钠、氯化钾、碳酸氢钢、葡萄糖等合理配伍,制成好得快饮水剂,在三黄种鸡、艾维茵商品肉鸡、雉鸡、鹧鸪、朱斯鸡、猪、牛、犬等畜禽中的临床应用表明,好得快以1:20溶于常水中,令畜禽自由饮用,可有效防治鸡白痢、鸡大肠杆菌、仔猪副伤寒、猪传染性胃肠炎、犊牛副伤寒、犬胃肠炎、中暑、幼畜腹泻等疾病,治愈率达90%以上.在鸡的传染性法氏襄炎、盲肠肝炎、大肠杆菌病、新城疫等多种病中,配合使用好得快,可明显缩短病程,提高临床治愈率,对防制肠道多种细菌感染和继发,并发症效果显著.