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1.
Cecilia Y. Kato Chifumi Nagai Paul H. Moore Francis Zee Minna S. Kim Denise L. Steiger Ray Ming 《Genetic Resources and Crop Evolution》2005,51(8):815-825
Pineapple (Ananas comosus (L.) Merr.) cultivars, often derived from somatic mutations, are propagated vegetatively. It has been suggested by isozyme data that there is little genetic variation among Smooth Cayenne cultivars. A thorough investigation of the genetic variation within the cultivated speciesAnanas comosus, particularly among commercial cultivars, will provide critical information needed for crop improvement and cultivar protection. One-hundred and forty-eight accessions ofA. comosus and 14 accessions of related species were evaluated with AFLP markers. The average genetic similarity ofA. comosus was 0.735 ranging from 0.549 to 0.972, suggesting a high degree of genetic variation within this species. With AFLP markers, discrete DNA fingerprints were detected for each commercial cultivar, breeding line, and intra-specific hybrid. Self-incompatibility, high levels of somatic mutation, and intraspecific hybridization may account for this high degree of variation. However, major cultivar groups of pineapple, such as Cayenne, Spanish, and Queen, could not be distinctively separated. These cultivar groups are based on morphological similarity, and the similar appearance can be caused by a few mutations that occurred on different genetic background. Our results suggest that there is abundant genetic variation within existing pineapple germplasm for selection, and discrete DNA fingerprinting patterns for commercial cultivars can be detected for cultivar protection. The genetic diversity and relationships of fourAnanas species are also discussed. 相似文献
2.
Abdelhameed Elameen Siri Fjellheim Arild Larsen Odd Arne Rognli Leif Sundheim Susan Msolla Esther Masumba Kiddo Mtunda Sonja S. Klemsdal 《Genetic Resources and Crop Evolution》2008,55(3):397-408
Sweet potato (Ipomoea batatas L.) is the fifth most important crop in the developing countries after rice, wheat, maize and cassava. The amplified fragment
length polymorphism (AFLP) method was used to study the genetic diversity and relationships of sweet potato accessions in
the germplasm collection of Sokoine University of Agriculture, Morogoro and Sugarcane Research Institute, Kibaha, Tanzania.
AFLP analysis of 97 sweet potato accessions using ten primer combinations gave a total of 202 clear polymorphic bands. Each
one of the 97 sweet potato accessions could be distinguished based on these primer combinations. Estimates of genetic similarities
were obtained by the Dice coefficient, and a final dendrogram was constructed with the un-weight pair-group method using arithmetic
average. AFLP-based genetic similarity varied from 0.388 to 0.941, with a mean of 0.709. Cluster analysis using genetic similarity
divided the accessions into two main groups suggesting that there are genetic relationships among the accessions. Principal
Coordinate analysis confirmed the pattern of the cluster analysis. Analysis of molecular variance revealed greater variation
within regions (96.19%) than among regions (3.81%). The results from the AFLP analysis revealed a relatively low genetic diversity
among the germplasm accessions and the genetic distances between regions were low. A maximally diverse subset of 13 accessions
capturing 97% of the molecular markers diversity was identified. We were able to detect duplicates accessions in the germplasm
collection using the highly polymorphic markers obtained by AFLP, which were found to be an efficient tool to characterize
the genetic diversity and relationships of sweet potato accessions in the germplasm collection in Tanzania. 相似文献
3.
Fábio Luís Maciel Sergio Echeverrigaray Lee Tseng Sheng Gerald Felipe Gobbi Grazziotin 《Genetic Resources and Crop Evolution》2003,50(8):887-893
The genetic variation and relationships among 31 accessions of Phaseolus vulgaris L., and two representatives of Vigna unguiculata L., were evaluated by AFLP analysis. A total of 263 DNA fragments across all materials were scored using nine primer combinations, averaging 32 per primer. More than 95% of the amplification products showed polymorphism, indicating high variation at the DNA level among these accessions. Pair-wise genetic similarity (Jaccard's coefficient) ranged from 0.553 to 0.840, with a mean of 0.765. Twenty-three accessions (70%) clustered into three groups. A majority of the commercial cultivars (91%) clustered within a single group, whereas the landraces were distributed along all the variation. An apparent correlation with phaseolin types was detected. Results of this study suggest that Brazilian landraces truly represent the overall genetic variability of Phaseolus vulgaris, confirming the multiple origins of these materials, and their potential as a source of variation for breeding programs. 相似文献
4.
Norihiko Tomooka Mun Sup Yoon Koji Doi Akito Kaga Duncan Vaughan 《Genetic Resources and Crop Evolution》2002,49(5):521-530
A representative set of germplasm of genusVigna subgenus Ceratotropis specieswas used to analyze genetic diversity using amplified fragment lengthpolymorphism (AFLP) methodology. The germplasm was selected based onmorphological diversity and geographic origin and includes 18 out of the 21species in the subgenus Ceratotropis. Genetic diversitywithin and between taxa was measured using information generated by AFLP bandpolymorphism from which Jaccard's similarity coefficient and nucleotidediversity were estimated. The data were also used to generate phenograms tovisualize relationships among analyzed accessions. All species in sectionsCeratotropis and Aconitifoliae arewell separated. However, most of the species in sectionAngulares show a high level of similarity suggesting a lowlevel of genetic differentiation. In section Angulares onespecies, V. trinervia, is welldifferentiated from the other species and represents a separate evolutionarybranch between section Ceratotropis and other species insection Angulares. The relationships between the newlydescribed species V. aridicola,V. exilis, V.nepalensis and V.tenuicaulis and other species in the subgenusCeratotropis are described for the first time based on AFLPdata. 相似文献
5.
Forty-seven samples of date palm (Phoenix dactylifera L.) collected from eight locations in Egypt were studied using four sets of amplified fragment length polymorphism (AFLP)
markers with near infrared fluorescence labeled primers. These samples belonged to 21 named accessions and 9 of unknown pedigrees.
A total of 350 bands were scored and 233 (66.6%) were polymorphic. Twenty-seven Egyptian accessions and ‘Medjool’and ‘Deglet
Noor’accessions from California could beclassified into the major cluster. This major cluster may represent a major group
of date palm germplasm in North Africa. There were four other clusters, each containing one or two accessions. The variety
‘Halawy’and one accession of unknown provenance were most likely from hybridization between two clusters. Six groups of accessions
of which had the same names, revealed similar but not identical AFLP profiles suggesting these accessions might derive from
seedlings rather thanthrough clonal offshoot propagation. 相似文献
6.
Pedro Talhinhas José Leitão João Neves-Martins 《Genetic Resources and Crop Evolution》2006,53(3):563-578
Lupinus angustifolius L. is a Mediterranean species, domesticated in the 20th century, representing an important grain legume crop in Australia
and other countries. This work is focused on the collection of wild germplasm and on the characterisation of morphological
and molecular diversity of germplasm accessions. It reports the collection of 81 wild L. angustifolius accessions from the South and Centre of Portugal, available at the ‘Instituto Superior de Agronomia Gene Bank’, with subsequent
morphological and molecular characterisation of a selection of these and other accessions. A multivariate analysis of morphological
traits on 88 L. angustifolius accessions (including 59 wild Portuguese accessions, 15 cultivars and 14 breeding lines) showed a cline of variation on wild
germplasm, with plants from Southern Portugal characterised by earlier flowering, higher vegetative development and larger
seeds. AFLP and ISSR molecular markers grouped modern cultivars as sub-clusters within the wider diversity of wild germplasm,
revealing the narrow pool of genetic diversity on which domesticated accessions are based. The importance of preserving, characterising
and using wild genetic resources for L. angustifolius crop improvement is outlined by the results obtained. 相似文献
7.
Sergio Lanteri Alberto Acquadro Luciana Quagliotti Ezio Portis 《Genetic Resources and Crop Evolution》2003,50(7):723-735
In several regions of Italy as well as other parts of southern Europe, the heterogeneity of the land, the climate and the soil favour the survival in cultivation of a large number of landraces specifically adapted to local conditions. Knowledge on the level and distribution of their genetic variation can help to develop appropriate strategies, in order to suistainably manage in situ these germplasm resources at risk of genetic erosion. C. annuum is an herbaceous diploid species and is considered to be self-pollinating, although different rates of out-crossing have been recorded. We used random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers to assess genetic diversity within and between five populations of a landrace of Capsicum annuum L., grown in a limited area in north-west Italy and locally known as Cuneo pepper. Partitioning the genetic variation with Shannon's diversity index revealed that 41.6% occurred between and 58.4% within populations. Analogous results were obtained when the analysis was based only on RAPD or AFLP markers. However, AFLP was more reliable, since a lower range of variation was observed among primer combinations in detecting the two components of genetic variation. Notwithstanding the rather high level of within genetic variation detected, the five populations were clearly differentiated and differed in the frequency of alleles exclusive and/or present at very low frequencies. Our results show the need for accurate estimation of allele frequencies, in order to identify populations to which priority should be given for dynamic conservation of landraces. 相似文献
8.
Jinggui Fang Chih-Cheng T. Chao Philip A. Roberts Jeffrey D. Ehlers 《Genetic Resources and Crop Evolution》2007,54(6):1197-1209
Cowpea is an important grain legume and hay crop of many tropical and subtropical regions, especially in the dry savanna region
of West Africa. The cowpea gene pool may be narrow because of a genetic bottleneck during domestication. Genetic variation
within specific breeding programs may be further restricted due to breeding methods, ‘founder effects’ and limited exchange
of germplasm between breeding programs. Genetic relationships among 60 advanced breeding lines from six breeding programs
in West Africa and USA, and 27 landrace accessions from Africa, Asia, and South America were examined using amplified fragment
length polymorphism (AFLP) markers with six near infrared fluorescence labeled EcoRI + 3/1bases/MseI + 3/1bases primer sets. A total of 382 bands were scored among the accessions with 207 polymorphic bands (54.2%). Despite
a diverse origin, the 87 cowpea accessions shared a minimum 86% genetic similarity. Principal coordinates analysis showed
clustering of breeding lines by program origin, indicating lack of genetic diversity compared to potential diversity. Accessions
from Asia and the Americas overlapped and were distinct from West African breeding lines, indicating that germplasm from Asia
and the Americas have common origins outside West Africa. US and Asian breeding programs could increase genetic variability
in their programs substantially by incorporating germplasm from West Africa, while national programs in West Africa should
consider introgression of Asian germplasm and germplasm from other parts of Africa into their programs to ensure long-term
gains from selection. 相似文献
9.
Nature of taro (Colocasia esculenta (L.) Schott) genetic diversity prevalent in a Pacific Ocean island, Vanua Lava, Vanuatu 总被引:1,自引:0,他引:1
S. Caillon J. Quero-Garcia J.-P. Lescure V. Lebot 《Genetic Resources and Crop Evolution》2006,53(6):1273-1289
Taro (Colocasia esculenta (L.) Schott), cultivated in Vêtuboso, a village of northern Vanuatu, Melanesia, was surveyed to: (1) assess the extent of morphological and molecular variation being maintained by growers at the village level and, (2) compare this diversity with the diversity found in the crops in Vanuatu. Ethnobotanical data were combined with AFLP analysis to elucidate possible sources of variation. Folk assessment of variation is based on: (a) morphological characteristics (11 characters), (b) names and (c) classification according to habitat, uses, origin and agronomic adaptation. This 3-fold approach allowed growers to differentiate 96 morphotypes, all of which are given distinct vernacular names. AFLP fingerprints successfully differentiated all these 96 morphotypes which do not present a significant intra-clonal variation. But genetic results showed no clear groupings according to geographic origin or habitat of morphotypes and stated that the diversity found within the village was comparable with the overall diversity found in Vanuatu. Local nomenclature and stories associated with each cultivar suggested three sources of diversity: introductions (38%), somatic mutations (15%) and sexual recombinations (48%). AFLP results confirm folk beliefs about origin at least for three pairs of mutants. The 11 so-called wild forms analysed by AFLP were suggested to be feral, escapes from domestication. A dynamic in situ conservation strategy (DISC), favouring a broadening of the national genetic base, was discussed for taro. 相似文献
10.
C. Tara Satyavathi K. V. Bhat C. Bharadwaj S. P. Tiwari V. K. Chaudhury 《Genetic Resources and Crop Evolution》2006,53(5):1069-1079
AFLP technique was used to assess genetic diversity in 72 soybean varieties under cultivation in India. Selected 12 AFLP primer
pairs produced 1319 products of which 1257 were polymorphic (95%). Wide variations were observed for the number of amplification
products, percent polymorphism and average polymorphism information content (PIC). The Jaccard's similarity indices (J) based on the AFLP profiles of the 72 soybean varieties were subjected to UPGMA cluster analysis. The dendrogram generated
revealed four major clusters, which were strongly supported by the high bootstrap values obtained from analyses of 1000 bootstrap
samples. In addition, the Mantel's test for cophenetic correlation with r = 0.955 indicated very good fit of the varieties to a group in the cluster analysis. Some correspondence between the clustering
pattern and the pedigree, place of release or target area of the variety was observed. Overall moderately high genetic diversity
was observed which appears to be due to the higher genetic diversity prevalent in 12 of the varieties included in three diverse
clusters and was indicative of the need to include more diverse germplasm in the soybean improvement programs. 相似文献
11.
Worapa Seehalak Norihiko Tomooka Aree Waranyuwat Piyada Thipyapong Paisan Laosuwan Akito Kaga Duncan A. Vaughan 《Genetic Resources and Crop Evolution》2006,53(5):1043-1059
Thailand is a center of diversity for section Angulares of the Asian Vigna (genus Vigna subgenus Ceratotropis) and 4 Vigna species are cultivated in Thailand. Using newly collected wild and cultivated germplasm of Vigna from Thailand and outgroup accessions AFLP analysis was conducted to clarify genetic diversity and relationships. The results
suggest that cultivated V. umbellata and V. mungo evolved from wild relatives in a single domestication event. Vigna umbellata is poorly differentiated from its wild and weedy relatives compared to V. mungo. Results suggest northern Thailand and the neighboring Shan state, Myanmar, is the probable center of domestication for V. umbellata as wild accessions from this area and cultivated rice bean from a wide area in Asia are not greatly diverged. Vigna minima, V. tenuicaulis and V. exilis accessions in Thailand are well differentiated with considerable intra-specific variation. Vigna hirtella consists of two well differentiated subgroups, suggesting taxonomic revision may be necessary. Close genetic relationships
between V. radiata and V. grandiflora, and between V. mungo and V. trinervia are confirmed. Naturally growing V. mungo populations in northern Thailand appear to be true wild species as they are well differentiated from Indian wild and Thai
cultivated populations. The origin of naturally growing cowpea in Thailand needs to be further studied using a more comprehensive
set of materials. This study clarifies inter and intra-specific genetic diversity and inter species relationships of Thai
Vigna species. 相似文献
12.
Target region amplification polymorphism (TRAP) markers were used to assess genetic variability among 38 germplasm accessions
and 10 commercial hybrids of spinach (Spinacia oleracea L.), an economically important leafy vegetable crop in many countries. Germplasm accessions with different geographic origins
and 10 commercial hybrids were examined. For assessing genetic diversity within accessions, DNA was extracted from 12 individual
seedlings from six germplasm accessions and two hybrids. A relatively high level of polymorphism was found within accessions
based on 59 polymorphic TRAP markers generated from one fixed primer derived from the Arabidopsis-like telomere repeat sequence and two arbitrary primers. For evaluating interaccession variability, DNA was extracted from
a bulk of six to 13 seedlings of each accession. Of the 492 fragments amplified by 12 primer combinations, 96 (19.5%) were
polymorphic and discriminated the 48 accessions from each other. The average pair-wise genetic similarity coefficient (Dice)
was 57.5% with a range from 23.2 to 85.3%. A dendrogram indicated that the genetic relationships among the accessions were
not highly associated with the geographic locations in which the germplasms were collected. The seven commercial hybrids were
grouped in three separate clusters, suggesting that the phenotype-based breeding activities tended to reduce the genetic variability.
This preliminary study demonstrated that TRAP markers are effective for fingerprinting and evaluating genetic variability
among spinach germplasms.
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information
and does not imply recommendation or endorsement by the US Department of Agriculture.
The U.S. Government's right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged. 相似文献
13.
Genetic diversity among some important Syrian wheat cultivars was estimated using Amplified Fragment Length Polymorphism (AFLP)
markers. Five Triticum aestivum L. and 10 Triticum turgidum ssp. durum were analyzed with 11 EcoRI–MseI primer pair combinations. Of the approximately 525 detected AFLP markers, only 46.67% were polymorphic. Cluster analysis
with the entire AFLP data divided all cultivars into two major groups reflecting their origins. The first one contained T. aestivum L. cultivars, and the T. turgidum ssp. durum cultivars and landraces were grouped in the second. Narrow genetic diversity among all cultivars was detected with an average
genetic similarity of 0.884. The lowest similarity index (0.9) was found between Cham5 and Hamary (durum wheat), whereas this value was 0.93 between Salamony and Bouhouth 4 (T. aestivum L.). The narrow genetic diversity level indicates that these genotypes could be originated from the same source. AFLP analysis
provides crucial information for studying genetic variation among wheat cultivars and provides important information for plant
improvement. 相似文献
14.
Genetic diversity and intra-specific phylogeny of Triticum turgidum L. subsp. dicoccon (Schrank) Thell. revealed by RFLPs and SSRs 总被引:2,自引:5,他引:2
Today, emmer wheat, T. turgidum subsp. dicoccon, widely grown in the past is a candidate crop for sustainable agriculture in Italy. As part of a research project aimed at the enhanced use of the hulled wheat germplasm, molecular characterization was carried out to understand the genetic structure of the crop and to identify accessions of interest. A collection of 194 accessions was analyzed with 15 microsatellite loci (SSRs), while only a sample of 38 accessions was tested with 19 RFLP probes. The marker loci were selected on the basis of their independent genomic distribution. Genetic distances and allelic frequencies were calculated for each marker class. The genetic relationships were visualized with dendrograms. RFLP loci were, on average, less polymorphic than SSRs. An average Dice's genetic distance of 0.22 for RFLPs vs 0.38 for SSRs was detected, while an expected average heterozygosity per locus of 0.23 for RFLPs vs 0.26 for SSRs was also estimated. With a least number of 10 loci per marker class it was possible to identify each genotype. The most diverse accessions had different geographic origins. Germplasms from Italy and Ethiopia appear to belong to a more primitive genepool, given that a group of accessions from these countries were genetically differentiated from a Russian-Iranian group. 相似文献
15.
M. Costea D. M. Brenner F. J. Tardif Y. F. Tan M. Sun 《Genetic Resources and Crop Evolution》2006,53(8):1625-1633
The ‘Morelos’ accessions of Amaranthus from Mexico demonstrate taxonomic ambiguity at the basic morphologic level. The main cause is the enormous morphological
and genetic variation exhibited by the species in the genus. Although basic morphological criteria can be applied to herbarium
specimens or germplasm collections for quick taxonomic identification, the morphological data alone can be misleading. To
ascertain the taxonomic identity of the ‘Morelos’ accessions and their hypothesized species affiliation to Amaranthus caudatus or Amaranthus cruentus, we conducted a comparative analysis of phylogenetic relationships among these taxa/accessions using amplified fragment length
polymorphism (AFLP) and micromorphology methods. Based on AFLP data, all the controversial ‘Morelos’ accessions can be consistently
placed into a single A. cruentus species clade, which is clearly separated from the A. caudatus species clade. The AFLP-based phylogenetic relationship of ‘Morelos’ and delimitation of A. cruentus and A. caudatus are further supported by micromorphology, showing that the combination of these techniques can provide more reliable data
for germplasm identification than each method used alone. 相似文献
16.
Salhi-Hannachi Amel Trifi Mokhtar Zehdi Salwa Hedfi Jihène Mars Messaoud Rhouma Abdelmajid Marrakchi Mohamed 《Genetic Resources and Crop Evolution》2004,51(3):269-275
The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving power value of 29.6. A total of 47 discernible fragments were scored from samples, with a mean of 11.7 fragments per primer. The 90.4% of sample that were polymorphic were scored as molecular markers to examine the Tunisian fig germplasm polymorphism at DNA level. A large genetic diversity as related to ISSR patterns was found within the local Tunisian fig germplasm. An UPGMA dendrogram exhibits the unstructured variability in this crop. Moreover, the principal component analysis shows that the observed diversity was typically continuous. Our data provide a large number of ISSR markers that are useful in the fingerprinting of Ficus
carica L. cultivars, and in the understanding of the genetic relationships among these accessions. 相似文献
17.
N. Grati-Kamoun F. Lamy Mahmoud A. Rebaï A. Gargouri O. Panaud A. Saar 《Genetic Resources and Crop Evolution》2006,53(2):265-275
About 29 olive (Olea europaea L.) cultivars including oil and table olive cultivars originating from Tunisia and other Mediterranean countries, were genotyped
using amplified fragment length polymorphism (AFLP) DNA markers. This technique is a rapid and efficient method for producing
DNA fingerprints. Using nine AFLP primer combinations, we produced a total of 410 AFLP markers, among which 172 revealed polymorphism.
The results demonstrated a high degree of polymorphism in the olive germplasm we examined with an average of 39%. These AFLP
markers were analyzed to estimate genetic distances between pairs of cultivars using Jaccard’s similarity coefficient. Furthermore,
cluster and principal component analyses were performed in order to identify the genetic variation patterns. Two main groups
were obtained: one comprising primarily small-fruited cultivars grown mainly for oil production and the other comprising large
fruited cultivars (regardless of their end-use). Our results show no evidence of clustering of olive cultivars according to
their geographic origin. 相似文献
18.
F. Shan H. J. Clarke G. Yan J. A. Plummer K. H. M. Siddique 《Genetic Resources and Crop Evolution》2007,54(3):519-527
Wild annual Cicer gene pools contain valuable germplasm for chickpea improvement programs. Previous research showed that duplication might
exist in accessions collected from these gene pools, which would hinder chickpea breeding and related research. AFLP (amplified
fragment length polymorphism) markers were used to fingerprint the world collections of the primary and secondary gene pools
including C. reticulatum Lad., C. bijugum K.H. Rech., C. judaicum Boiss. and C. pinnatifidum Jaub. et Sp. Duplicates were detected in a total of 24 accessions in both the gene pools, highlighting the necessity to fingerprint
the germplasm. Genotypic difference was detected as gene pool specific, species specific and accession specific AFLP markers.
These were developed into fingerprinting keys for accession identification between and within species and gene pools. Use
of AFLP markers to detect duplicates and to identify accessions is a reliable method which will assist in the characterisation
and use of wild annual Cicer germplasm in chickpea improvement programs. We recommend the procedure presented in this paper as a standard approach for
the precise genetic identification and characterisation of future world collections of wild Cicer, to keep germplasm integrity and to benefit chickpea breeding and related research programs. 相似文献
19.
V. Sérgio Emílio Dos Santos M. Aparecido Gimenes J. Francisco Montenegro Valls C. Romero Lopes 《Genetic Resources and Crop Evolution》2003,50(8):841-848
Some Arachis species are widely used as commercial plants, e.g. the groundnut A. hypogaea, an important source of good quality protein and oil, and A. pintoi and A. glabrata, that are utilized as forage species. Germplasm of most Arachis species is available in germplasm banks. However, little it is known about the genetic attributes of this germplasm, and mainly about its genetic variability, which is very important for its maintenance. In the present study RAPDs were used to assay the genetic variation within and among 48 accessions of five sections of the genus Arachis and to establish the genetic relationships among these accessions. Ten of 34 primers tested were selected for DNA amplification reactions since they yielded the largest numbers of polymorphic loci. A dendrogram was constructed based on data from the 10 primers selected. Eighty RAPD polymorphic bands were analyzed among the accessions studied. The relationships among species based on RAPDs were similar to those previously reported based on morphological, cytological and crossability data; demonstrating that RAPDs can be used to determine the genetic relationships among species of the different sections of the genus Arachis. In general, wide variation was found among accessions and low variation was found within the accessions that had two or more plants analyzed. However, higher polymorphism was found in the section Trierectoides and in one accession of A. major, indicating that generalizations should be avoided and each species should be analyzed in order to establish collection and maintenance strategies. 相似文献
20.
Shao-Qing Tang Xiao-Yun Bin Yun-Tao Peng Jun-Ya Zhou Li Wang Yang Zhong 《Genetic Resources and Crop Evolution》2007,54(5):1053-1061
Genetic variation of wild populations and cultivars of Luohanguo (Siraitia grosvenorii), a plant species endemic to southern China, was assessed using random amplified polymorphic DNA (RAPD) and amplified fragment
length polymorphism (AFLP) markers. Based on the results for 130 individuals from seven populations, a high level of genetic
diversity of Luohanguo was observed at the species level. The percentage of polymorphic loci (P) was 89.4%, Nei’s gene diversity (H
e) was 0.239, and Shannon’s information index (H
o) was 0.373 based on the combined AFLP and RAPD data. There was a high degree of genetic differentiation, with 45.1% of the
genetic variation attributed to differences between the populations. The genetic diversity of the Luohanguo cultivars is much
lower than that of wild populations (P = 41.8%, H
e = 0.141, H
o = 0.211), and a distinct genetic differentiation is observed between the cultivars and wild accessions. The pool of genetic
variation in the wild populations provides an excellent gene resource for Luohanguo breeding. 相似文献