Studies on the Maturity of Apples.: Respiration Progress Curves for Cox’s Orange Pippin Apples for a Number of Consecutive Seasons

Summary

The effect of UV-B irradiation at 10°C and 20°C on the quercetin glycosides procyanidins, chlorogenic acid and anthocyanin levels in the skin of detached fruit of five apple cultivars was investigated. UV-B responsiveness was compared between sides of the fruit that were outwardly facing (exposed) and inwardly facing (shaded) when on the tree. There were no common effects of UV-B irradiation and temperature across all cultivars. UV-B irradiation increased the quercetin glycoside levels in the shaded side of ‘Gala’, ‘Royal Gala’ and ‘Braeburn’ fruit and only at 20°C. UV-B irradiation of the exposed sides of the five cultivars, of either side at 10°C, and of ‘Pacific Rose’ and ‘Aurora’ at either 10°C or 20°C had no significant effect on quercetin glycoside levels. There was no effect of UV-B irradiation on procyanidin levels at any combination of treatments. However, levels of chlorogenic acid, although one tenth of those of quercetin glycosides and procyanidins, increased markedly with UV-B irradiation. Both exposed and shaded sides of fruit of all cultivars increased in chlorogenic acid levels at 10°C and 20°C after UV-B irradiation. UV-B irradiation increased anthocyanin levels in exposed and shaded sides of fruit of all cultivars at 20°C, and on ‘Braeburn’, and ‘Aurora’ at both 20°C and 10°C. UV-B irradiation at either temperature had no significant effect on either the relative proportions of the glycosides of quercetin (galactoside, rhamnoglucoside, glucoside, xyloside, arabinoside and rhamnoside) or the relative proportions of the procyanidins (Procyanidins B2, B5, epicatechin and catechin). The effect of UV-B irradiation was variable, depending on cultivar, previous light exposure, temperature and class of flavonoids examined.     

Spatial aspects of metapopulation survival – from model results to rules of thumb for landscape management

The role of spatial configuration for metapopulation survival is analyzed by using a stochastic metapopulation model. This model reveals conditions which must be satisfied by the species' ecology and the landscape settings before a metapopulation can persist over a long term. Taking this as a basis, initial rules of thumb for landscape management are deduced. The following results are highlighted: (1) the critical correlation length d_c of the extinction processes determines a spatial scale of the metapopulation dynamics. (2) Only species with a dispersal range d_r above the correlation length d_c are able to benefit from landscape management at all. (3) A certain metapopulation can only persist over a long term if no patch is inside the range of correlation of another one. (4) There is a hierarchy of importance in the characteristics of a spatial configuration (scale and type) and, hence, in the scopes of landscape management. To conclude, some general consequences for supporting species survival by management are discussed. Some prospects concerning the use of models for decision support in landscape planning are discussed.     

Optimization of Fermentation Medium for Cordyceps brasiliensis Using Response Surface Methodology

The composition of fermentation medium for producing Cordyceps brasiliensis mycelium was optimized using response surface methodology. A Plackett-Burman design was applied to screen potential key factors selected on the basis of preliminary experiments, and the steepest ascent method was adopted to approach the approximate optimal medium composition. Finally, central composite design and response surface methodology were applied to define more precisely the optimal composition of the fermentation medium. Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium (100 mL) was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.     

Analysis of S-alleles by PCR for determination of compatibility in the ‘Red Delicious’ apple orchard

Summary

In a previous study, the apple ‘Jonathan’ was shown to be semi-compatible with the ‘Topred’ sport of ‘Red Delicious’, the main cultivar in Israel. This incompatibility led to reduced pollination potency, explaining the low yield obtained from ‘Topred’ rows adjacent to ‘Jonathan’. Hence, the importance of identifying the level of compatibility for all ‘Topred’ pollinators became apparent. By analysing S-allele by PCR with primers for the various S-alleles of the cultivars in the ‘Topred’ orchard, including S10 and S28, which were cloned in this study, it was evident that apart from ‘Jonathan’, the common pollinators for ‘Topred’ are fully compatible with it. The ability to identify all S-alleles in the ‘Topred’ orchard was also utilized to resolve the observation that, although ‘Topred’ is considered self-incompatible, self-pollinated, net-caged, ‘Topred’ flowers set some fruit. Applying PCR-analysis of S-alleles to the progeny of the suspected self-pollinated ‘Topred’ fruit revealed that the fruit-set was the outcome of cross-pollination, perhaps the consequence of fertilization by wind carried pollen. Hence, the notion that ‘Topred’ is totally self-incompatible was confirmed and the efficiency of progeny PCR S-allele analysis for the determination of the pollen donor was exhibited.     

Pocket parks for people – A study of park design and use

The present study focuses on small urban public parks, specifically on how they should be designed to promote a potential for psychological restoration and on what activities people could imagine doing in such parks. Students from Oslo University College were presented with visual stimuli depicting 74 small urban parks. They were asked to assess how likely it was that they could rest and recover in the parks presented in the photos. In addition, the participants were asked to write down what park components contributed to high versus low ratings on restoration likelihood, and what types of activities they could imagine doing in the parks presented in the photos. The components and activities the participants mentioned were grouped into categories describing similar properties. The categories mentioned most frequently and giving high ratings on restoration likelihood represented natural categories such as: ‘a lot of grass’, ‘a lot of flowers/plants’ and ‘water features’. In contrast, the categories contributing to low ratings on restoration likelihood represented lack of vegetation or disturbance from the surroundings such as: ‘a lot of hard surfaces’, ‘a lot of traffic’ and ‘poorly shielded from the surroundings’. The activities mentioned as being typically performed in the types of parks presented in the photos were ‘relax and philosophize’, ‘read’ and ‘eat/drink’. Conclusions drawn from the study: Small urban parks should be designed with natural components, shielded from disturbing surroundings and furnished with some seating to promote opportunities for restorative experiences and to function as social meeting places.     

Models of upland species’ distributions are improved by accounting for geodiversity

Context

Recent research suggests that novel geodiversity data on landforms, hydrology and surface materials can improve biodiversity models at the landscape scale by quantifying abiotic variability more effectively than commonly used measures of spatial heterogeneity. However, few studies consider whether these variables can account for, and improve our understanding of, species’ distributions.

Objectives

Assess the role of geodiversity components as macro-scale controls of plant species’ distributions in a montane landscape.

Methods

We used an innovative approach to quantifying a landscape, creating an ecologically meaningful geodiversity dataset that accounted for hydrology, morphometry (landforms derived from geomorphometric techniques), and soil parent material (data from expert sources). We compared models with geodiversity to those just using topographic metrics (e.g. slope and elevation) and climate data. Species distribution models (SDMs) were produced for ‘rare’ (N?=?76) and ‘common’ (N?=?505) plant species at 1 km² resolution for the Cairngorms National Park, Scotland.

Results

The addition of automatically produced landform geodiversity data and hydrological features to a basic SDM (climate, elevation, and slope) resulted in a significant improvement in model fit across all common species’ distribution models. Adding further geodiversity data on surface materials resulted in a less consistent statistical improvement, but added considerable conceptual value to many individual rare and common SDMs.

Conclusions

The geodiversity data used here helped us capture the abiotic environment’s heterogeneity and allowed for explicit links between the geophysical landscape and species’ ecology. It is encouraging that relatively simple and easily produced geodiversity data have the potential to improve SDMs. Our findings have important implications for applied conservation and support the need to consider geodiversity in management.

     

Use of organic agricultural wastes as growing media for the production of Anthurium andraeanum ‘Pink Lady’

Summary

The conventional organic growing medium used for anthurium (Anthurium andraeanum) production is peat. Quality peat, however, is costly and is a non-renewable natural resource. The current study compared the suitability of using charred and composted organic agricultural wastes (OAW) vs. sphagnum peat as components of various growth media for the ornamental plant, anthurium (A. andraeanum ‘Pink Lady’). Ten different media were prepared from composted corn cobs (C), charred corn cobs (CC), charred garden waste (CG), and Pindstrup? sphagnum peat moss (P) in various proportions. The media contained [on a % (v/v) basis]: C (100%), CC (100%), CG (100%), C (70%) + CG (30%), CC (70%) + CG (30%), C (35%) + CG (15%) + P (50%), CC (50%) + P (50%), CC (35%) + CG (15%) + P (50%), C (50%) + P (50%), or P (100%) as a control. After 15 months in a greenhouse, the growth and flowering of transplanted anthurium plants were optimal using P, CC+P, or CC+CG+P.These results indicate that mixing charred OAW with peat could provide a suitable medium for anthurium production, thereby reducing the need for peat and converting agricultural waste material into useful products.     

The Potential of Caprifig Genotypes for Sheltering <Emphasis Type="Italic">Blastophaga psenes</Emphasis> L. for Caprification of Edible Figs

Caprifig (Ficus carica L.) as pollen sources, play a major role in edible fig caprification by Blastophaga psenes L. The present study was carried out to evaluate the potential of three caprifig genotypes (Poozdombali, Kouhi and Kouhaki) for the shelter of B. psenes wasp in Estahban region, Fars province, Iran. Summer (profichi) crop fruits per genotype were harvested in full maturity period and evaluated for five times for the shelter of B. psenes wasp. Significant differences among caprifig genotypes were found for B. psenes number for the three times carried out (0, 21 and 28 days), but they did not show significant differences for two times (7 and 14 days). At day of harvesting, Poozdombali showed the highest B. psenes wasp number (341.33) and Kouhaki had the lowest (200.00) and they showed significant differences for this character in this time. Also, seven days after harvesting, Poozdombali showed the highest wasp number (106.67) and Kouhi and Kouhaki had the lowest (104.33), but not significant difference. Number of B. psenes at the day of harvesting was the highest for all of the genotypes and at 28 days after harvesting was the lowest. Cluster analysis identified two major clusters with several sub-clusters. Caprifig trees with having high B. psenes number are essential for a suitable caprification. Thus, Poozdombali genotype with the highest B. psenes number, is suitable in this important trait to be used for edible figs caprification.     

Allerton Park 1983: the beginnings of a paradigm for landscape ecology?

In 1983, a group of incipient landscape ecologists met to discuss the nature and future directions for landscape ecology. The themes emerging from this conference—movement of materials, organisms, and energy through a landscape; the genesis of landscape patterns; the effects of landscape structure on the spread of disturbances; and the potential contributions of landscape ecology to resource management—established a foundation for the development of landscape ecology in North America over the following decades. I discuss these contributions in the light of where landscape ecology is today.

The Hamburg Tree Pruning System – A framework for pruning of individual trees

Trees in urban areas are pruned for several reasons. Each pruning cut causes a wound, which leads at least in the exposed wood at the wound surface to discoloration and decay. The extension of the resulting defect depends on wound diameter, tree species, time of wounding, wound treatment, and last but not least the attachment of the branch to the stem. The effects of different pruning cuts on urban trees has been investigated in Hamburg, Germany, since 1985. The study of 750 different pruning wounds on typical urban tree species lead to the Hamburg Tree Pruning System (Hamburger Schnittmethode). The system is described in this paper and answers the following questions: How should branches with and without a branch collar be removed? How should dead branches be removed? How should branches with included bark or codominant stems be removed? What are the differences between tree species in wound response? What is the maximum wound size which will be effectively compartmentalised by the tree?     

Molecular evidence for the natural hybrid origin of Phalaenopsis × intermedia Lindl.

Summary

In order to confirm the hybrid origin of Phalaenopsis intermedia Lindl., internal transcribed spacers (ITSs) of ribosomal DNA (rDNA) and three fragments of chloroplast DNA (cpDNA) were studied. Nineteen clones of ITS sequences from three accessions of P. intermedia Lindl. were aligned with nine species of sections of Phalaenopsis and Stauroglottis that were candidate parents. A phylogenetic tree, derived from the ITS data, was constructed by the Neighbor-joining (NJ) method. Two major groups were shown for 19 clones of ITS sequences of P. intermedia based on the phylogenetic tree. The average genetic distance between the aforementioned two groups and the candidate parents was calculated based on the Kimura 2-parameter method. One group had the lowest genetic distance from the ITS repeat sequences of P. aphrodite Rchb.f., and another group had the lowest distance from that of P. equestris (Schauer) Rchb.f. The results indicated that both P. aphrodite and P. equestris are parents of P. intermedia based on the ITS data. In addition, analysis of three fragments of cpDNA, namely the trnL intron, the trnL-trnF intergenic spacer (IGS), and the atpB-rbcL IGS, showed that P. intermedia had the lowest genetic distance from P. aphrodite. Both the ITS and cpDNA data, as well as reference to the effects of maternal inheritance of cpDNA, suggest that P. aphrodite was the maternal parent and P. equestris was the paternal parent of P. intermedia. Therefore, molecular evidence supports P. intermedia being a natural hybrid derived from P. aphrodite and P. equestris.     

Development of a dot blot technique for rapid identi®cation of Botrytis cinerea,the causal organism of grey mould in greenhouse tomatoes

Summary

A dot blot assay, based on the internal transcribed spacer of the ribosomal region, was developed for rapid diagnosis and identification of Botrytis cinerea, the causal agent of grey mould of greenhouse tomatoes. In this assay, DNA from fungal cultures and diseased plant tissue were amplified by PCR using primers specific to septate fungi, fixed to nylon membranes, and hybridized with digoxigenin-d-UTP labelled oligonucleotides specific to B. cinerea. The hybridized probes were detected by chemiluminescence. Six isolates of B. cinerea, four isolates of Botrytis species, one isolate each of B. fabae, B. squamosa, B. streptothrix, B. allii, B. porri, and B. tulipae and some common greenhouse pathogens were tested using this assay. Of four probes screened, probe Bot1 gave a positive reaction with all isolates of B. cinerea and with fresh or frozen plant tissue affected with grey mould from research centres and commercial greenhouses. All other greenhouse fungi tested negative with probe Bot1. This dot blot diagnoses infection by B. cinerea in large numbers of samples rapidly and reliably and distinguishes isolates of B. cinerea from other epiphyte fungi found on greenhouse crops.     

Addressing the interplay of poverty and the ecology of landscapes: a Grand Challenge Topic for landscape ecologists?

We argue for the landscape ecology community to adopt the study of poverty and the ecology of landscapes as a Grand Challenge Topic. We present five areas of possible research foci that we believe that landscape ecologists can join with other social and environmental scientists to increase scientific understanding of this pressing issue: (1) scale and poverty; (2) landscape structure and human well-being; (3) social and ecological processes linked to spatial patterns in landscapes; (4) conservation and poverty, and (5) applying the landscape ecologist’s toolkit. A brief set of recommendations for landscape ecologists is also presented. These include the need to utilize broad frameworks that integrate social and ecological variables, build capacity to do this kind of work through the development of strong collaborations of researchers in developed and developing countries, create databases in international locations where extreme poverty exists, and create a new generation of researchers capable of addressing this pressing social and environmental issue.     

Optimization of a Sequence-Related Amplified Polymorphism (SRAP) Amplification System for Lentinula edodes

Lentinula edodesis a large,edible wood-decaying fungus that is attractive to consumersdue to its delicious taste and medicinalproperties.One of the major cultivatedmushrooms,it is ranked second(marginallybehindAgaricus bisporus)in terms of worldproduction.L.edodeswas originally cultivatedinChina,whichis nowthelargest producer of themushroom and rich in germplas m resources.These resources form the basis ofL.edodesbreeding programs and product exploitation.However,research on the genetic rela…     

Optimization of a Sequence-Related Amplified Polymorphism (SRAP) Reaction System for Lentinula edodes

Although China is now the largestproducer and exporter ofLentinula edodes,there is still considerable confusion in theChineseL.edodesindustry with regard to thedesignation of cultivatedstrains.Asingle strainis often named differently in differentcultivation areas,and different strains areoccasionally allotted the same strain desig-nation.However,various molecular markershave been developed in recent years and couldbe used to remove much of this confusion.Todate,the most widely used molecular…     

Role of vascular remodeling for urotensin Ⅱ in rat thoracic aorta after balloon injury

AIM: To explore the possible effect of UII in the process of remodeling after vascular injury. METHODS: The rat model of balloon injury in thoracic aorta was established. Male rats were randomized to 4 groups (n=5), including sham injury group, injury group, UII group (UII pumped into the rats after thoracic aorta balloon injury at 1.0 nmol·kg-1·h-1) and urantide group (urantide pumped into the rats after thoracic aorta balloon injury at 10 nmol·kg-1·h-1). At 21 days, the thoracic aortas were taken out to measure the changes of pathology, the expression of UII, the proliferation of VSMC and the expression of collagen. RESULTS: (1) At the 21 days after operations, the systolic blood pressure was higher in UII group than that in injury group ［(140.0±10.0) mmHg vs (132.0±3.4) mmHg, P>0.05］. The systolic blood pressure was also obviously higher than that in urantide group ［(140±10.0) mmHg vs (128.0±2.4) mmHg, P<0.05］. (2) Urotensin Ⅱ was expressed strongly in the injured area after thoracic aorta injury. (3) In contrast to injury group, the intimal thicken in urotensin Ⅱ group enhanced, the decrease in lumen area was marked (0.13±0.05 vs 0.07±0.02, P<0.05), the cell proliferation index was markedly increased (0.74±0.16 vs 0.40±0.11, P<0.01), and the expression of collagen was also markedly increased (counted as IOD, 318±127 vs 78±26, P<0.01). (4) In contrast to injury group, the decrease in lumen area was not abolished (0.09±0.03 vs 0.07±0.02, P>0.05) after chronic infusion of urotensin Ⅱ receptor antagonist urantide, the cell proliferation index was markedly increased (0.73±0.15 vs 0.40±0.11, P<0.01) and the expression of collagen was not statistically increased (counted as IOD, 200±79 vs 78±26, P>0.05). CONCLUSION: Urotensin Ⅱ expresses strongly in the myointimal cells after thoracic aorta injury in rat. The extra UII enhances the proliferation of VSMC and expression of collagen in the myointimal, increases the stenosis of injured vasculature, indicating that UII might take part in the process of repairing after vessel injury.     

The inhibition of nitric oxide synthase for long time up-regulates vascular angiotensin Ⅱ receptor

AIM:To observe the effects of aortal angiotensin Ⅱ(AngⅡ)levels and AngⅡ receptor in the hypertensive rat models. METHODS: Intraperinoneal injection of L-N^ω-nitro-arginine(L-NNA) into rats induced hypertensive model, the binding of aortal AngⅡ receptor and the contents of aortal tissue AngⅡ and plasma NO₂^-+NO₃^-(NOx) were determined.RESULTS:Compared with the control group, the blood pressure of the rats treated with L-NNA was significantly increased by 142%(P＜0.01),the plasma NOx levels were decreased by 48%. However, in the rats treated for 4 weeks the ratio of cardial index was increased by 128%(P＜0.01),the plasma AngⅡ levels weren't significantly changed, the contents of vascular tissue AngⅡ were increased by 612%(P＜0.01) and the Bmax of [¹²⁵I]-AngⅡ was increased by six times (P＜0.01),the affinity was doubled respectively.CONCLUSIONS:Nitric oxide synthase (NOS) inhibition mostly influes the reninangiotensin system in regional tissue. The inhibition for long time up-regulates vascular AngⅡ and AngⅡ receptor, which can support the hypothesis "NOS inhibition can induce AngⅡ-depended hypertension".     

Hopping on one leg – The challenge of ecosystem disservices for urban green management

The importance of ecosystem services for human well-being has been highlighted by several recent environmental studies and assessments. These assessments provide useful information for urban green management, but they largely exclude various nuisances and losses produced by ecosystem functions. Lack of attention to these ecosystem disservices may seriously hamper environmental management in general and urban green management in particular. Ecological, social and technological changes will affect both what kind of disservices will emerge and how they will be experienced and managed. Especially in urban areas, environmental, social and technological changes spanning from global to local levels are continuously altering urban ecosystems. Our ways of perceiving and valuing ecosystem functions are also undergoing rapid change. Here we discuss the concept of ecosystem disservices from the perspective of northern European urban ecosystems. It is concluded that perceptions about ecosystem disservices have an increasing influence on how urban green areas are experienced, valued, used, managed and developed.