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1.
为了克隆甘肃合作猪白细胞抗原(SLA)DRA和DRB基因,分析SLA-DR基因特性和抗原多态性,首次研究了甘肃合作猪在异种移植等研究领域中的应用前景.应用RT-PCR分别扩增合作猪SLA-DRA和SLA-DRB基因并进行测序,将所获得基因序列登录GenBank(登录号分别为FJ905823和FJ9058258),再用NCBI中的BLAST和ExPASy等相关软件进行生物信息学分析.发现在猪-人异种移植中,近亲繁殖的合作猪种在与人类主要组织相容性复合体遗传基因水平相似性方面有一定优势,也可作为备选猪种对相关基因进行必要的修饰和改造.  相似文献   

2.
非洲猪瘟病毒微滴数字PCR检测方法的建立   总被引:2,自引:0,他引:2  
建立一种具有高灵敏度的检测非洲猪瘟病毒的微滴数字PCR方法,为非洲猪瘟的快速诊断和流行病学研究提供技术支持。在《OIE陆生动物诊断与疫苗手册》中提供的qPCR检测方法的基础上,建立了非洲猪瘟病毒微滴数字PCR方法,对该方法的敏感性、特异性和重复性进行了评估。结果显示,该方法的最低检测限可达0.8copies/μL,敏感性高于qPCR方法,不与猪常见的6种病毒发生交叉反应,而且重复性较好。采用建立的微滴数字PCR和OIE的qPCR方法分别对78份临床病料进行非洲猪瘟病毒的检测,结果显示2种方法的符合率为100%。本研究建立的微滴数字PCR方法具有理想的敏感性和特异性,适用于临床上非洲猪瘟病毒的检测。  相似文献   

3.
五指山猪白细胞抗原血清学研究   总被引:4,自引:0,他引:4  
用24支标准血清对25头五指山猪进行白细胞抗原的血清学分型,根据标准微量淋巴细胞毒试验结果,按国际SLA-A、C、B抗原血清学分型标准、共检出21种单倍型,另有8条、占16%的单倍型不能确定。在所检出的单倍型中,H4、H32含量较高,频率分别达0.12和0.10;H9、H10、H17、H23、H35、H42、H53等单倍型为中等频率分布,频率从0.04 ̄0.08;H1、H2、H3、H6、H8、H2  相似文献   

4.
猪水疱病病毒研究进展   总被引:1,自引:0,他引:1  
猪水疱病是猪的一种急性传染病,与口蹄疫和水疱性口炎等水疱类疫病具有同等的重要性,被世界动物卫生组织(OIE)列为A类疾病。文章主要介绍了本病病原近年来在分子生物学方面的研究概况,主要包括RNA基因组的结构和功能的研究、结构蛋白功能的新发现、抗原结构的研究、病毒的诊断研究以及与柯萨奇病毒B5的关系等方面的研究现状。  相似文献   

5.
贺灿  张彬 《猪业科学》2020,37(11):96-98
非洲猪瘟是养猪业的第一杀手,自1921年东非国家肯尼亚首先出现非洲猪瘟疫情以来,这个病毒已经在非洲亚洲欧洲等区域传播了近百年,对世界养猪业造成了无可估量的损失.我国于2018年8月在辽宁省沈阳市首次发生非洲猪瘟疫情,并在极短的时间内传遍全国,对我国养猪行业的发展产生了巨大的影响,养猪业在近2年时间里发生了极大的变化.文...  相似文献   

6.
The major histocompatibility complex (MHC) of cattle is known as the bovine leukocyte antigen (BoLA) and is located on chromosome 23. BoLA has been linked to variation in resistance to disease including bovine leukemia virus‐induced lymphoma and mastitis. Moreover, BoLA appears to influence other traits such as milk yield, growth and reproduction, which are not often measured in humans, and variations in individual immune response to antigen. The BoLA appears to be organized in a similar way to the MHC region in humans, but there are notable differences. A major rearrangement within the class II region has led to the division of the BoLA into two distinct subregions of chromosome 23 separated by about a third of the chromosome’s length. The class IIa subregion contains functionally expressed DR and DQ genes, while the class IIb subregion contains the genes of undefined status such as DYA, DYB, DMA, DMB, DOB, DOA, TAP1, TAP2, LAP2 and LMP7. In addition, one pair of human class II genes (DP) does not appear to have an equivalent in cattle, and there is one pair of DY genes that seem to be found only cattle, sheep and goats. In humans, three classical, polymorphic class I genes (HLA‐A, ‐B and ‐C ) are each present on all haplotypes. However, in cattle, none of the four (or more) classical class‐I genes identified are consistently expressed, and haplotypes differ from one to another in both the gene number and composition. These variations in both class I and II are likely to play an important role in cattle immune responses. This review summarizes current knowledge of the structural and functional features and disease association of BoLA genes.  相似文献   

7.
8.
一例猪链球菌2型感染的病原分离鉴定及药敏试验   总被引:1,自引:0,他引:1  
2016年2月,上海市崇明区某乡镇一规模养猪场出现40多头5~8周龄仔猪发病死亡情况。经细菌分离、分离菌株生化鉴定和PCR检测,确诊为猪链球菌2型感染。对该菌株采用K-B纸片法,进行10种常用抗菌药物的体外药敏试验,结果发现该细菌对青霉素、阿莫西林、恩诺沙星敏感,对诺氟沙星、阿奇霉素、壮观霉素、卡那霉素、阿米卡星、庆大霉素和氨苄西林不敏感。根据药敏试验结果,建议养猪户使用阿莫西林和恩诺沙星进行治疗,并做好病猪的隔离与护理。一周后病情得到控制。  相似文献   

9.
肇东市某商品猪场内仔猪大批发病,病猪精神状态极差、发热、食欲下降、拉白色糊状并伴有腥臭味的粪便,有些迅速死亡但死亡率不高。为确诊此批病猪,运用临床分子生物学诊断和病理解剖观察等手段初步判定为大肠杆菌与猪瘟病毒混合感染。根据其剖解现象使用细菌学检测和PCR技术对蓝耳病病毒、猪圆环病毒、猪瘟病毒、大肠杆菌、放线杆菌及链球菌进行检测。SS培养基培养出的菌落形态为圆形半透明的小凸起,革兰氏染色镜检后观察到是革兰氏阴性杆菌。PCR检测结果显示猪瘟病毒为阳性。最终确诊为大肠杆菌和猪瘟病毒混合感染。通过对分离细菌进行药敏试验结果显示该菌对环丙沙、硫酸阿米卡星、恩诺沙星、阿莫西林等药物较为敏感。  相似文献   

10.
  总被引:2,自引:0,他引:2  
The recent availability of the full‐length sequence of one haplotype of the swine leukocyte antigen (SLA) complex, the swine major histocompatibility complex (MHC), and significant progress in the studies on gene expression and polymorphisms led to major advances in deciphering its role in resistance to diseases in animals. The present status of the genomic organization and polymorphism of the SLA complex is presented in this Review. Additionally, a comparative analysis with mammalian MHC has also been provided. The sequenced SLA‐H01 haplotype harbors 152 loci including genuine SLA genes, non‐MHC genes and pseudogenes. Although the numbers of expressed SLA genes could vary across haplotypes, three SLA class Ia, three SLA class Ib, four SLA class IIa and four SLA class IIb genes are currently expressed. Except for the class I genes, which have no clear orthologs, the gene organization of the loci was highly conserved between humans and pigs. Moreover, the human leukocyte antigen (HLA) complex lies on a single chromosomal segment, whereas a centromere at the class II and III junction splits the SLA complex into two segments, without disturbing gene organization or impeding functionality. Over 400 SLA class I and II allele sequences available in databases have been recently clustered and assigned to a specific SLA locus according to a newly defined nomenclature system.  相似文献   

11.
为构建烟台黑猪SLA-2-YTH基因原核表达载体,本研究设计引物PCR扩增SLA-2-YTH胞外区,将其克隆至pMD 19-T Simple 载体,筛选阳性克隆。阳性克隆经酶切后,进一步与表达载体pET-28a(+)连接,转化BL21(Rosseta)感受态细胞并进行诱导表达,SDS-PAGE检测蛋白表达情况。结果显示,SLA-2-YTH胞外区亚克隆大小为834 bp,酶切鉴定证实其成功插入pET-28a(+)表达载体。SDS-PAGE结果显示,SLA-2-YTH基因导入宿主菌后成功表达,蛋白大小约31.0 ku,与预期结果相符,优化后蛋白相对表达量达25%以上。本研究成功构建了烟台黑猪SLA-2-YTH原核表达载体,获得了表达蛋白,为今后进一步的结构和功能研究奠定基础。  相似文献   

12.
13.
柴伟东 《猪业科学》2020,37(2):90-92
猪口腔液作为血清、鼻腔拭子和肛拭子的替代品,已被用来检测和监测包括非洲猪瘟病毒在内的多种传染病.由于口腔液样品自身的复杂性,使得口腔液样品中的病原微生物容易降解,导致检测结果的不准确.试验的目的是分析动物DNA病毒(PRV)和RNA病毒(PRRSV)在口腔液样品中的储运条件对病原核酸检测结果的影响.将PRV和PRRSV...  相似文献   

14.
地锦草注射液的临床疗效试验表明:该制剂对仔猪黄、白痢的总有效率为86.1%,与其他对照药物的疗效比较,差异显著(P〈0.05);对猪感冒病的总有效率为83.1%,与其他对照药物的疗效比较,差异极显著(P〈0.01)。  相似文献   

15.
用13/17染色体易位杂合子猪互交获得的1头13/17染色体易位纯合子公猪[2n=36,XY,rob(13/17)]与4头正常核型母猪(2n=38,XX)交配,结果4头母猪全部妊娠并正常分娩.其产仔数分别为13,16,12,11,产活仔数分别为12,13,12,11;窝断奶头数分别为12,13,9,10.经核型鉴定,其后代全部为13/17易位杂合子[2n=37,XX或XY,rob(13/17)].此结果表明,13/17易位染色体的遗传完全符合孟德尔的遗传规律,13/17易位纯合子公猪具有正常的繁殖力.本杂交试验所产活仔48头,其性别比例为35?:13?,接近3:1的比例,经X~2检验不符合1:1的理论比率(X~2=10.08,P<0.01).此外,本杂交试验所产仔猪生长发育正常,并具有一定的杂合优势,这一结果为13/17易位纯合子猪的进一步推广应用,尤其是作为商品猪生产的杂交父本提供了可靠依据.  相似文献   

16.
    
A 19-year-old Quarter Horse gelding was evaluated for respiratory distress and a rapidly enlarging retropharyngeal mass. Initial evaluation revealed severe respiratory distress, and a large, firm mass, visibly appreciable as 12 × 12 cm, in the left retropharyngeal and perilaryngeal region, with surrounding left and right retropharyngeal swelling. No significant abnormalities were present on complete blood count and serum biochemistry analyses. Endoscopy revealed severe pharyngeal collapse restricting airflow without gross abnormalities of the pharyngeal mucosa other than inflammation and irritation. A multilobular retropharyngeal mass, diffusely heterogeneous in echogenicity, was present adjacent to, but not occluding, the carotid artery as assessed by ultrasonography. Initial needle aspirate suggested lymphoma. Tissue biopsy and histopathology confirmed a round cell tumour. A temporary tracheotomy was performed to provide respiratory relief, and the horse was managed on oral antibiotics and anti-inflammatory medications while awaiting histopathological results. The decision was made to humanely euthanise the horse after biopsy results indicated lymphoma. Definitive diagnosis of T cell rich, large B cell lymphoma was made by combination of cytology, immunohistochemistry and molecular clonality PCR (PARR) testing. Lymphoma should be considered in horses with focal masses of the retropharyngeal region. Although treatment was not pursued, PARR testing was successful in this case and may be helpful for accurate characterisation of lymphoma in horses to more precisely determine prognosis and the most effective treatment plans, as it has been in human patients and small animals.  相似文献   

17.
谈永松  王林云 《养猪》2003,(6):25-27
根据国内外最新资料对猪MHC Ⅲ类区域的基因及功能、物理图谱等方面进行了综述,以期对猪MHC Ⅲ类基因的研究提供最新学术动态。  相似文献   

18.
猪MHCⅡ类区域基因研究进展   总被引:2,自引:0,他引:2  
本文综述了国内外有关猪MHCⅡ类基因的研究进展,特别是对Ⅱ类区域基因的结构、功能、类型、多态及分型方法、Ⅱ类等位基因间的关系等方面进行了较为详尽的阐述。  相似文献   

19.
应用Primer3.0和Omega2.0,根据猪伪狂犬病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)和猪流感病毒(SIV)保守基因设计了3对多重PCR引物,建立PRRSV,SIV和PRV单项PCR检测方法,并在优化单项PCR反应条件(引物浓度、Mg2 浓度、退火温度等)基础上,初步建立了PRRSV-PRV-SIV多重PCR检测方法,并分别用多重PCR和单项PCR/RT-PCR检测10份临床病料,两者符合率为96.6%,表明该多重PCR检测方法有较高的敏感度,可以用于临床病料的检测。  相似文献   

20.
呼吸型鸡传染性支气管炎病毒的抗原定位与动态分布   总被引:1,自引:0,他引:1  
应用间接免疫荧光抗体(IFA)和RT-PCR法对人工感染IBV-M41株的SPF鸡不同脏器中的病毒进行了跟踪检测。结果在感染后24h气管中最早出现特异性荧光,感染后第3h肺脏和肾脏出现特异性荧光,感染后第5d肝脏、脾脏、法氏囊、直肠等脏器也不同程度的出现特异性荧光,特异性荧光在气管中最强,持续时间也最长,可达14d或更长:在肺脏和肾脏可持续3~5d;而在其他器官持续1~3d。试验结果表明气管、肺脏、肾脏都是M41株病毒增殖的场所,但随感染时间的延长,病毒最终定位于主要的靶器官气管。IFA和RT-PCR方法均可以对IBV抗原定位,相对来说IFA法更直观,而RT-PCR更灵敏。  相似文献   

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