Desiccation-induced damage to the cell envelope of root-nodule bacteria

Leakage of materials reacting with the fluorescent probe 1-anilino-8-naphthalcne sulphonate (ANS) from root-nodule bacteria subjected to desiccation and rehydration suggests that death of these bacteria is associated with changes in membrane permeability. The slow-growing rhizobia and the fast-growing Rhizobium meliloti differ from other fast-growing bacteria of the R. leguminosarum group in that they exhibit little fluorescence in the presence of ANS. This probably reflects differences between these groups of bacteria in the polar/non-polar nature of the outer cell envelopes. Following desiccation and rehydration, all groups of root-nodule bacteria fluoresced strongly in the presence of ANS.Repair of desiccation-induced damage to the cell envelopes of R. japonicum QA372. R. trifolii SU297/32B and R. meliloti CC131 was not observed using a range of screening agents. However, repair of significant numbers of lysozyme-sensitive bacteria was observed with R. leguminosarum TA101 within an hour of rehydration.     

Some factors affecting survival of sclerotia of Macrophomina phaseolina in soil

At least 75% of the sclerotia of Macrophomina phaseolina survived for 1 yr in most natural soils kept at 26°C and at 50–55% of the soil moisture holding capacity (m.h.c.). Although survivability was reduced in a very acid soil (pH 4.5) collected under a pine stand, 33% of the sclerotia survived for 1 yr. Soil pH had very little or no effect on sclerotial survivability. Of three organic amendments tested (alfalfa hay, chitin, pine needles) only ground alfalfa hay at 0.8% (w/w) reduced survivability of sclerotia in soil by about 75% in a year. Alfalfa hay at 0.4% reduced survivability by 36%. Various N sources added at 200 μg Ng^?1 soil had no effect on survival. Of 13 fungicides tested, only benomyl and captan at 20 μg a.i. g^?1 soil appreciably reduced populations of sclerotia in soil.Soil temperature and moisture content were the two most important factors affecting survivability of sclerotia. At ?5 or 5°C the biggest drop in sclerotial survivability occurred when the soil was incubated moist (at 50% m.h.c. or more). At 26°C the biggest drop occurred in air-dried soil (2–3% m.h.c.) and survivability was decreased to some extent at 15 and 30% m.h.c. Survivability also dropped rapidly in moist soil (50–55% m.h.c.) exposed to four cycles each having 3-week freezing (?5°C) and 1 week thawing (26°C). Sclerotia in air-dried soil (2–3% m.h.c.) continuously kept at ?5°C maintained nearly complete survivability after 16 weeks. Sclerotia survived almost 80–90% in moist soil (50–55% m.h.c.) kept for 16 weeks at 26°C or in moist soil exposed to four cycles each having 3-week thawing (26°C) and 1-week freezing (?5°C).     

Estimation of number of root-nodule bacteria by a nodulation-dilution frequency method

Rhizobia spend most of their life in the soil outside the host plants. Soil conditions, therefore, may exert influence on the survival and variation of rhizobia so as to contribute to the nitrogen fixation of the legumes. The knowledge on the ecology of Rhizobium in soils may expected to give basis for the success of seed inoculation and establishment of effective strains in the field. In addition, it will render service for the study of the ecological soil microbiology, for example, ecology of pathogens of soil-borne plant diseases.     

Some factors affecting behaviour of boron in soil

It has been reported by many workers that various soil properties influence the retention of boron added to soils, but there is little infomration on the relative importance of these properties to boron retention and there is something controversial in the published results regarding the effects of different soil properties such as organic matter content, soil reaction, available calcium content and texture on boron retention (15). The present study was undertaken to obtain more detailed informations on the relationships between boron adsorption and different properties of soils, and on comparative contribution of soil constituents such as organic matter, sesquioxides and inorganic colloids to boron adsorption of soils. In Japan, boron deficiency symptoms of crops often appear in the fields of volcanic ash soils, and many experiments on boron application have been conducted to amend the boron deficiencies of the soils. It is considered that volcanic ash soils may have special characteristics concerning boron retention in comparison with nonvolcanic ash soils. In the present study, therefore, some volcanic ash soils were also taken as samples in addition to non-volcanic ash soils to confirm their speciality to boron retention.     

Survival of soil bacteria during prolonged desiccation

A determination was made of the kinds and numbers of bacteria surviving when two soils were maintained in the laboratory under dry conditions for more than half a year. Certain non-spore-forming bacteria were found to survive in the dry condition for long periods. A higher percentage of drought-tolerant than drought-sensitive bacteria was able to grow at low water activities. When they were grown in media with high salt concentrations, bacteria generally became more tolerant of prolonged drought and they persisted longer. The percent of cells in a bacterial population that remained viable when exposed to drought stress varied with the stage of growth.     

Symbiotic nitrogen contribution and biodiversity of root-nodule bacteria nodulating Psoralea species in the Cape Fynbos,South Africa

The genus Psoralea (tribe Psoraleae, family Leguminosae) is indigenous to the Cape fynbos of South Africa and little is known about its symbiosis and/or adaptation. The aim of this study was to assess root nodulation and N₂ fixation in eight of the 50 Psoralea species, as well as the biodiversity of their associated nodulating microsymbionts. The eight species studied (namely, Psoralea pinnata, Psoralea aphylla, Psoralea aculeata, Psoralea monophylla, Psoralea repens, Psoralea laxa, Psoralea asarina and Psoralea restioides) all had round-shaped, determinate type (desmodioid) nodules, and data from ¹⁵N natural abundance showed that they obtained 60–88% of their N nutrition from symbiotic fixation. These Psoralea species also transported their fixed-N as ureides (allantoin and allantoic acid) in the xylem stream, a symbiotic trait that links them very closely to the tribe Phaseoleae. Bacteria isolated from root nodules of the eight Psoralea species varied in phenotypic characteristics, nodulation promiscuity, and N₂-fixing efficacy. Furthermore, 16S rDNA gene sequence data showed that Psoralea species can form root nodules with different soil bacteria, including Rhizobium, Mesorhizobium and Burkholderia strains. This is not only evidence of nodulation promiscuity, but also an indication of the species’ adaptation to the nutrient-poor, low-N, sandy acidic soils of the Cape fynbos.     

Some factors affecting the interlamellar adsorption of alcohols by 2:1 clay minerals

Homoionic forms of montmorillonites and a vermiculite were prepared and their charge characteristics examined. Clay-alcohol complexes of these homoionic systems were studied by means of differential thermal micro-analysis (DTMA), X-ray and infrared techniques. The interlamellar adsorption of alcohols by montmorillonites appears to be independent of the net layer charge, the source of the charge, the type of the saturating cation and the predrying of the homoionic forms examined.The vermiculite used in this study was found to have 1 : 1 biotite-vermiculite mixed layers with a net layer charge of 1.72 mainly located in the tetrahedral position. The type of saturating cation in such a mineral plays a major role in its expansion and in adsorption of alcohols.     

Some factors affecting regional differentiation of the soils in the Republic of Benin (West Africa)

Spatial distribution of the parent materials, color, clay-eluviated horizon thickness, textural profile type, presence and position of the ferruginous nodules and ferricretes in the profile are analyzed at a small scale using the thematic information content of the published 1:200,000 soil map of Benin. Analyses indicate that there is a regional differentiation of the soil cover which is first drawn on that of the geological provinces, sedimentary basins, great morpho-structural units, and then determined by the factors that control the preservation or the transformation and erosion of an ancient lateritic mantle. These factors are directly influenced by differential changes of the local base levels.     

Some factors affecting the intensity and locus of expression of mineral deficiency and toxicity symptoms by plants

Abstract

Using yields of plants obtained at various times of the year and from probable assumptions relative to sugar production, solute sorption, and solute distributions between roots and tops it has been deduced that both deficiencies and toxicities in mineral nutrition would be expected to be revealed earlier or more prominently in a summer season. Light is the primary determining environmental factor. The locus of nutrient deficiency revelation depends on the remobility of the element in the plant; toxic disorder is usually revealed in the oldest foliar parts.     

土壤微量元素含量及其影响因素的研究

论述了临沂市土壤中硼、锌、锰、铜、铁等5种主要微量元素的状况与其它因素的关系。有效硼含量0.09~3.67 mg/kg,平均0.35 mg/kg;有效锰22~572 mg/kg,平均244 mg/kg;有效锌0.15~4.02 mg/kg,平均0.53 mg/kg;有效铜0.09~5.78 mg/kg,,平均1.03 mg/kg;有效铁3.2~162 mg/kg,平均21.5 mg/kg;有效锰2.0~131.4 mg/kg,平均23.4 mg/kg。不同土壤类型的以上5种微量元素有明显差异,其特征是砂姜黑土缺锌,棕壤、水稻土富含铁、铜、锰、锌;成土母质是影响土壤微量元素的重要因素之一,发育在基性岩上的土壤一般含量较高,而由红土母质发育的土壤则含量较低。土壤有机质含量与土壤微量元素有明显正相关关系,其中速效锌、速效硼和速效铜与有机质的关系尤为显著。     

土壤微量元素含量及其影响因素的研究

论述了临沂市土壤中硼、锌、锰、铜、铁等5种主要微量元素的状况与其它因素的关系。有效硼含量0．09～3．67mg／kg，平均0．35mg／kg；有效锰22～572mg／kg，平均244mg／kg；有效锌0．15～4．02mg／kg，平均O．53mg／kg；有效铜0．09～5．78mg／kg，平均1．03mg／kg；有效铁3．2～162mg／kg，平均21．5mg／kg；有效锰2．0～131．4mg／kg，平均23．4mg／kg。不同土壤类型的以上5种微量元素有明显差异，其特征是砂姜黑土缺锌，棕壤、水稻土富含铁、铜、锰、锌；成土母质是影响土壤微黾元素的重要因素之一，发育在基性岩上的土壤一般含量较高，而由红土母质发育的土壤则含量较低。土壤有机质含量与土壤微量元素有明显正相关关系，其中速效锌、速效硼和速效铜与有机质的关系尤为显著。     

Use of aliette,a basipetally translocated antimicrobial compound,to enhance rhizophere colonization and nodulation by root-nodule bacteria

Summary A method was developed to improve the colonizing ability of inoculated strains of root-nodule bacteria using aliette (aluminum tris-O-ethyl phosphonate), a basipetally translocated fungicide. Aliette applied to seeds of alfalfa inoculated with an aliette-resistant strain of Rhizobium meliloti increased the numbers of R. meliloti in the rhizosphere after 3 but not 37 days, increased the number of nodules, and with some seed treatments, increased the growth of alfalfa. The enhanced colonization by R. meliloti as a result of seed treatment with aliette lasted for at least 31 days for alfalfa, although plant weights did not increase, Colonization by R. meliloti was further enhanced if seeds and foliage were treated with the fungicide. Coating seeds or sparaying the foliage with aliette also increased the number and weight of nodules and nitrogenase activity in soybeans inoculated with an aliette-resistant strain of Bradyrhizobium japonicum. The stimulation of B. japonicum in the rhizosphere and of nodulation was evident with successive plantings of soybeans if the seeds for each planting were treated with the chemical, but aliette did not increase the yield of inoculated soybeans in the subsequent plantings. With only the seeds of the first planting of inoculated soybeans treated with aliette, the numbers of B. japonicum in the rhizosphere of subsequent plantings were only occasionally greater and the numbers of nodules on the later plantings were not increased. We suggest that root colonization, nodulation, and N₂ fixation by Rhizobium and Bradyrhizobium may be enhanced by the use of basipetally translocated antimicrobial compounds together with root-nodule bacteria that are resistant to those compounds.     

甘蔗切割器切割质量影响因素的试验研究

为了降低甘蔗切割器切割甘蔗的宿根破头率(后面简称破头率),提高切割器的切割质量,该文采用二次回归正交旋转组合试验方法及试验台试验、建立数学模型,研究了甘蔗切割器切割质量的影响因素,同时,对甘蔗切割器切割质量的影响因素进行了综合优化。结果表明试验因素对切割质量有较大的影响,试验因素综合优化结果能有较降低破头率,在干地或湿地切割甘蔗时,破头率小于14%,有效提高了切割器的甘蔗切割质量。     

肉牛甲烷排放影响因素的试验研究

为考察不同粗饲料类型、日粮精粗饲料比、能量摄入水平等因素对肉牛甲烷排放量的影响,从而为控制甲烷排放提供理论依据,本研究选用4头18月龄的健康肉用公牛(鲁西黄牛),针对不同粗饲料类型、饲料精粗比和日粮能量摄入水平进行了试验,采用SF₆示踪法对甲烷排放量进行了测定。试验结果表明：SF₆示踪法可用来快速测定反刍动物的甲烷排放量;影响肉牛甲烷排放量因素的主次顺序依次为：饲料粗料类型＞饲料精粗比＞能量摄入水平;对肉牛饲喂紫花苜蓿和青贮玉米秸比饲喂稻草和干玉米秸时甲烷排放量要小;日粮中粗饲料越多,则肉牛的甲烷排放量越大;随着能量摄入水平的加大,肉牛的甲烷排放量也呈增加的趋势。     

弯、直蔗剥叶质量影响因素的试验

采用二次通用旋转组合设计、数理统计分析方法及计算机优化技术,以直、弯蔗为研究对象,对影响剥叶质量的主要因素进行室内模拟剥叶试验,建立相应的数学模型,研究各因素及交互作用对剥叶质量的影响规律及机理,同时对甘蔗剥叶机剥叶质量影响因素进行了优化。结果表明,试验的各因素对直蔗和弯蔗剥叶质量影响规律存在较大的差异,试验因素的综合优化结果能有效提高剥叶质量,直蔗含杂率和折断率分别为0～0.78%和0～8.43%,弯蔗含杂率和折断率分别为0～0.5%和0～18%。     

柴油机颗粒捕集器热再生的影响因素研究

为研究柴油机颗粒物捕集器（diesel particulate filter,DPF）再生升温过程中排气热管理策略对柴油机氧化催化器（diesel oxidation catalyst,DOC）入口温度、发动机性能及污染物排放的影响,该研究分别选取低速低负荷、低速中负荷及中速低负荷工况,通过试验研究进气节流和喷油控制参数对DOC入口温度、燃油经济性及排放性能的影响。试验结果表明：通过进气节流、推迟后喷正时和增大后喷油量能够有效提高DOC入口温度,主喷正时和喷油压力对DOC入口温度的影响较小。基于Box-Behnken试验设计与响应曲面法对低速低负荷工况下进气节流耦合后喷策略的排气热管理策略进行多目标优化,以进气量、后喷正时和后喷油量为因子,DOC入口温度、有效燃油消耗率（brake specific fuel consumption,BSFC）、氮氧化合物（nitrogen oxides,NOx）和烟度排放为优化目标。响应曲面分析结果表明：各因素对DOC入口温度的影响程度从大到小为进气量、后喷油量、后喷正时;对BSFC和NOx排放的影响程度从大到小为后喷油量、后喷正时、进气量;对烟度排放的影响程度从大到小为进气量、后喷油量、后喷正时。当后喷正时为上止点后30 ℃A、进气量为87 kg/h、后喷油量为6 mg时,DOC入口温度达到最高,此时BSFC为275.4 g/(kW·h),NOx及烟度排放分别为7.38 g/(kW·h)和1.85 mg/m³。优化后最佳进气量、后喷正时和后喷油量分别为87 kg/h、29 ℃A和5.4 mg,与优化前相比,DOC入口温度提升43.9 ℃,BSFC增加31.8 g/(kW·h),NOx和烟度排放分别降低18%和29%。研究结果可为DOC入口温度优化控制提供参考。

     

影响原料乳品质的纤溶酶活性的因素分析

为了充分了解原料乳中纤溶酶活性,以便从源头上提高原料乳品质,该文针对中国原料乳的生产现状,分析了奶牛品种、胎次、养殖模式及挤奶时间对原料乳中纤溶酶活性的影响,并对纤溶酶的热稳定性进行了研究。结果表明,养殖模式、奶牛品种及胎次对原料乳中纤溶酶活性影响显著（P＜0.05）,牧场养殖、1、4胎和娟珊牛原料乳中纤溶酶活性显著低于养殖小区、2、3胎及荷斯坦牛乳中的纤溶酶活性;挤奶时间对原料乳中纤溶酶活性影响不显著（P＞0.05）;原料乳中纤溶酶活性随热处理温度的升高而逐渐下降,巴氏杀菌（75℃、15 s）仅可使原料乳中纤溶酶活性下降25%;半胱氨酸对乳中纤溶酶活性具有一定的抑制作用。研究结果对根据纤溶酶活性对原料乳进行品质评价及分级具有一定借鉴作用。     

Factors affecting transfer of degradative plasmids between bacteria in soils

Horizontal gene transfer is useful for enhancing bioremediation through gene bioaugmentation. However, factors affecting transfer of degradative plasmids have not been systematically addressed. To this end, plasmid transfer experiments were performed using a TOL-like plasmid carrying the gene encoding for catechol 2,3-dioxygenase (C23O) between two soil bacteria under different conditions. Transfer frequency increased with air temperature in the range of 10–35 °C and reached 6 × 10⁻⁴ transconjugants per donor cell at 35 °C. The transfer frequency detected at soil depth 5–10 cm was significantly higher (p < 0.05) compared with other depths. Addition of 5–75% LB in the microbial inoculum promoted plasmid transfer frequencies. Addition of phenol to the experimental system resulted in significantly higher transfer frequency (p < 0.05) compared with no addition. Transfer frequency heat-moisture in loam was significantly higher (p < 0.05) than in other soils. The highest transfer frequency was found in the experiment containing tomato seedlings, with up to about 1.3 × 10⁻³ transconjugants per donor cell. Corn and wheat seedlings also led to significantly higher transfer frequencies (p < 0.05) compared with no plants. Furthermore, C23O activities of transconjugants formed under different conditions were measured, as a surrogate measure of the activity of transconjugant. Transfer temperature, soil and plant types had a minor influence on activities of transconjugants. Topsoil (0–5 cm) transconjugants expressed C23O more efficiently under normal incubation condition, but less efficiently when soils incubated with excessive LB medium concentrations, and in the absence of phenol in soil. These results suggested that transfer temperature, soil depth, dilutions of LB broth, phenol content, and soil and plant types had important effects on transfer of the TOL-like plasmid in soil, and some factors also affected activities of transconjugants.     

光合菌群产氢量影响因素的研究

该文主要研究高效产氢光合菌群产氢的工艺条件，探讨光照度、温度、酸碱度、接种量等因素对光合菌群高效产氢过程的影响规律。结果表明，高效产氢光合菌群产氢必须在光照和厌氧的条件下进行，高效产氢光合菌群的良好产氢工艺条件为：光照度2000～6000 lx、温度28～34℃、pH值5～8、接种量10%～100%，最大产氢量达到39.6 mL H₂/g(COD)·d，对进一步研究开发太阳能光合生物制氢技术有重要的参考价值。     

影响土壤氮素径流流失的因素探析

从自然因素和人为因素出发探讨了地形、降雨、土壤理化性质、植被以及施肥、耕作制度等因素对土壤N素流失的影响,并综述了国内外有关研究的最新进展。并认为,基于这些因素相互作用的复杂性,有必要开展以下3方面的内容:一是大量开展多因子交叉实验,定量评价它们之间的关系,这为深入理解土壤N素流失机理和确定流域非点源污染模拟模型参数提供科学依据;二是加强有关土壤N素流失机理的多学科之间的联系,协同攻关建立较完善的理论认识;三是利用微观径流小区开展田间试验,结合遥感和GIS分析等方法,建立宏观大尺度土壤N素流失模型,为环境问题的宏观决策提供支持。