枸杞酒专用酿酒酵母的筛选及香气成分的分析

为筛选适合枸杞酒发酵的优良酿酒酵母,从枸杞自然发酵醪、枸杞叶及枸杞园土壤中采集样本。将分离的菌株形态初步鉴定为酿酒酵母的菌株通过杜氏小管发酵法、产酒精能力试验和耐受性试验,筛选出5株发酵性能好的酿酒酵母,以商业酵母 XR 作为对照,测定这5株菌所发酵枸杞酒的理化指标。运用顶空固相微萃取-气相色谱-质谱联用仪与香气活度值（OAV）分析果酒香气成分,筛选得到1株最佳酿酒酵母JY2,并进行分子鉴定。 结果表明：经菌株形态和26S rDNA的相似性比对后,JY2鉴定为酿酒酵母。JY2发酵的枸杞酒为金黄色,颜色饱满协调,澄清透明,口感柔和,香气馥郁。     

Characterization of laccase gene StLAC6 and its involvement in the pathogenicity and peroxisome function in Setosphaeria turcica

Laccases, as a kind of multicopper oxidase, play an important role in pigment synthesis and growth in fungi and are involved in their interactions with host plants. In Setosphaeria turcica, 9 laccase-like multicopper oxidases have been identified, and StLAC2 is involved in the synthesis of the melanin that accumulates in the cell wall. The function of another major laccase gene, StLAC6, was studied here. The knockout of StLAC6 had no effect on the growth, morphology or invasion ability of S. turcica, but the morphology and function of peroxisomes of knockout mutants were abnormal. The knockout of the StLAC6 gene resulted in increased contents of phenolic compounds and melanin and the sensitivity to fungicides increased compared with wild type strains. In the mutants of StLAC6, there is a significant change of the expression levels of other laccase genes. This study provides a new insight into laccase functions and the relationship of the laccase gene family in plant pathogenic fungi.     

Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize

Northern corn leaf blight （NCLB）, caused by the heterothallic ascomycete fungus Setosphaeria turcica, is a destructive foliar disease of maize and represents a serious threat to maize production worldwide. A comparative proteomic study was conducted to explore the molecular mechanisms underlying the defense responses of the maize resistant line A619 Ht2 to S. turcica race 13. Leaf proteins were extracted from mock and S. turcica-infected leaves after inoculated for 72 h and analyzed for differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry identification. 137 proteins showed reproducible differences in abundance by more than 2-fold at least, including 50 up-regulated proteins and 87 down-regulated proteins. 48 protein spots were successfully identified by MS analysis, which included 10 unique, 6 up-regulated, 20 down-regulated and 12 disappeared protein spots. These identified proteins were classified into 9 functional groups and involved in multiple functions, particularly in energy metabolism （46%）, protein destination and storage （12%）, and disease defense （18%）. Some defense-related proteins were upregulated such as 13-glucosidase, SOD, polyamines oxidase, HSC 70 and PPIases; while the expressions of photosynthesis- and metabolism-related proteins were down-regulated, by inoculation with S. turcica. The results indicated that a complex regulatory network was functioned in interaction between the resistant line A619 Ht2 and S. turcica. The resistance processes of A619 Ht2 mainly resided on directly releasing defense proteins, modulation of primary metabolism, affecting photosyntesis and carbohydrate metabolism.     

Melanin,DNA replication,and autophagy affect appressorium development in Setosphaeria turcica by regulating glycerol accumulation and metabolism

Setosphaeria turcica (syn. Exserohilum turcicum) is the pathogenic fungus of maize (Zea mays) that causes northern leaf blight, which is a major maize disease worldwide. Melanized appressoria are highly specialized infection structures formed by germinated conidia of S. turcica that infect maize leaves. The appressorium penetrates the plant cuticle by generating turgor, and glycerol is known to be the main source of the turgor. Here, the infection position penetrated by the appressorium on maize leaves was investigated, most of the germinated conidia entered the leaf interior by directly penetrating the epidermal cells, and the appressorium structure was necessary for the infection, whether it occurred through epidermal cells or stomata. Then, to investigate the effects of key factors in the development of the appressorium, we studied the effects of three inhibitors, including a melanin inhibitor (tricyclazole, TCZ), a DNA replication inhibitor (hydroxyurea, HU), and an autophagy inhibitor (3-methyladenine, 3-MA), on appressorium turgor and glycerol content. As results, appressorium turgor pressure and glycerol concentration in the appressorium reached their highest levels at the mature stage of the appressorium under the control and inhibitor treatments. The three inhibitors had the greatest effects on appressorium turgor pressure at this stage. Glycogen and liposomes are the main substances producing glycerol. It was also found inhibitors affected the distribution of glycogen and liposomes, which were detected in the conidia, the germ tube, and the appressorium during appressorium development. This study provides profound insight into the relationship between appressorium turgor pressure and glycerol content, which was affected by the synthesis of melanin, DNA replication, and autophagy in the developing appressorium during a S. turcica infection.     

MAP kinase gene STK1 is required for hyphal,conidial,and appressorial development,toxin biosynthesis,pathogenicity,and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica

The mitogen-activated protein kinase(MAPK), a key signal transduction component in the MAPK cascade pathway, regulates a variety of physiological activities in eukaryotes. However, little is known of the role MAPK plays in phytopathogenic fungi. In this research, we cloned the MAPK gene STK1 from the northern corn leaf blight pathogen Setosphaeria turcica and found that the gene shared high homology with the high osmolality glycerol(HOG) MAPK gene HOG1 of Saccharomyces cerevisiae. In addition, gene knockout technology was employed to investigate the function of STK1. Gene knockout mutants(KOs) were found to have altered hyphae morphology and no conidiogenesis, though they did show similar radial growth rate compared to the wild-type strain(WT). Furthermore, microscope observations indicated that STK1 KOs did not form normal appressoria at 48 h post-inoculation on a hydrophobic surface. STK1 KOs had reduced virulence, a significantly altered Helminthosporium turcicum(HT)-toxin composition, and diminished pathogenicity on the leaves of susceptible inbred corn OH43. Mycelium morphology appeared to be significantly swollen and the radial growth rates of STK1 KOs declined in comparison with WT under high osmotic stress. These results suggested that STK1 affects the hyphae development, conidiogenesis, and pathogenicity of S. turcica by regulating appressorium development and HT-toxin biosynthesis. Moreover, the gene appears to be involved in the hypertonic stress response in S. turcica.     

An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control

A protein kinase characterized by its ability to phosphorylate microtubule-associated protein-2 (MAP2), is thought to be an early intermediate in an insulin-stimulated phosphorylation cascade and in a variety of other mammalian cell responses to extracellular signals. A complementary DNA that encodes this protein serine-threonine kinase has been cloned, and the protein designated extracellular signal-regulated kinase 1 (ERK1). ERK1 has striking similarity to two protein kinases, KSS1 and FUS3, from yeast. The yeast kinases function in an antagonistic manner to regulate the cell cycle in response to mating factors. Thus, ERK1 and the two yeast kinases constitute a family of evolutionarily conserved enzymes involved in regulating the response of eukaryotic cells to extracellular signals.     

Identification and characterization of Pichia membranifaciens Hmp-1 isolated from spoilage blackberry wine

The pellicle-forming yeast could cause the quality deterioration of wine. In this study, a pellicle-forming strain Hmp-1 was isolated from the spoilage blackberry wine, and identified as Pichia membranifaciens based on the morphology and partial nucleotide sequence of 26S rDNA. The effects of fermentation conditions (ethanol, sulfur dioxide, sugar, and temperature) on the growth of P. membranifaciens strain Hmp-1 and Saccharomyces cerevisiae strain FM-S-115 (a strain used for the blackberry wine fermentation) were investigated, respectively. The results indicated that Hmp-1 had lower resistance to these factors compared to FM-S-115, and the growth of Hmp-1 was completely inhibited by 10% (v/v) or 50 mg L^–1 SO₂ during the fermentation of blackberry wine. These results suggested that Hmp-1 could effectively be controlled by increasing ethanol or SO₂ concentration during the fermentation and storage of blackberry wine. Furthermore, the analysis based on gas chromatography-mass spectrometry (GC-MS) showed that Hmp-1 remarkably decreased kinds of volatile compounds in blackberry wine, especially aldehydes and esters. In addition, some poisonous compounds were detected in the blackberry wine fermented by FM-S-115 and Hmp-1. These results suggested that Hmp-1 was a major cause leading to the quality deterioration of blackberry wine.     

Characterization of a blaCTX-M-3, blaKPC-2 and blaTEM-1B co-producing IncN plasmid in Escherichia coli of chicken origin

An extensively drug-resistant (XDR) Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui Province in China. Genomic analyses indicated that the strain 258E harbors an incompatibility group N (IncN) plasmid pEC258-3, which co-produces bla_CTX-M-3, bla_KPC-2, bla_TEM-1B, qnrS1, aac(6´)-Ib-cr, dfrA14, arr-3, and aac(6´)-Ib3. Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient. Furthermore, conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals, community and hospital settings.     

玉米大斑病菌STK2的基因组定位、蛋白质结构预测及其表达

【目的】确定玉米大斑病菌STK2在基因组中的位置;解析目的蛋白质Stk2的结构特征;构建STK2真核表达载体,获得真核表达体系中的胞外分泌蛋白。【方法】利用生物信息学方法,确定STK2在玉米大斑病菌基因组中的确切位置,解析Stk2蛋白质的结构特征;根据STK2的ORF序列及真核表达载体pPIC9K的多克隆位点设计引物,构建真核表达载体,采用电击转化法将重组质粒转入宿主菌 GS115 中进行诱导表达,利用SDS-PAGE检测并鉴定目的蛋白质。【结果】玉米大斑病菌STK2的ID为91433,该基因位于scaffold_3正链的1561986-1563262位置;Stk2蛋白具有MAPK类蛋白激酶的特征性保守结构域,其二级结构主要以α螺旋和无规则卷曲为主,β折叠较少且主要存在于N端,其三级结构具有1个较小的N端域和1个较大的C端域;成功构建了STK2的真核表达载体pPIC9K-STK2,筛选获得了抗性转化子;在毕赤酵母菌中,经甲醇诱导得到了1个分子量约为41 kD的蛋白质组分且被分泌至胞外,该蛋白质的大小与理论分子量相符,推测Stk2蛋白已在宿主菌中表达并被分泌至胞外。【结论】玉米大斑病菌STK2位于scaffold_3正链的1561986-1563262位置;Stk2具有MAPK激酶的所有特征性保守结构域,属于典型的MAPK蛋白激酶;该基因能在真核细胞中表达,获得了可溶性分泌蛋白质,为Stk2蛋白的多克隆抗体制备及基因的功能研究奠定基础。     

解淀粉芽孢杆菌F11抗真菌活性研究

解淀粉芽孢杆菌F11对核盘病菌、玉米大斑病菌、猕猴桃软腐病菌、烟草炭疽病菌等12种作物病原菌具有高效、广谱的抑菌效果,研究其生物防治机理可为该菌株应用于植物病害防治提供理论依据。本研究采用混菌法、牛津杯法及显微镜观察法探究了菌株F11发酵液不同稀释倍数、发酵液3种分离液（菌株发酵液离心分离获得上清液A,上清液A继而进行酸沉淀和甲醇提取分别获得发酵上清液B和胞外代谢物粗提液）对病原菌的抑制效果及抑菌特性。混菌法结果表明,菌株F11的抑菌效果随稀释倍数的增加而逐渐下降,但1 000倍稀释液对樟树炭疽病菌（Colletotrichum gloeosporioides）的相对抑制率和10倍稀释液与发酵原液对烟草黑胫病菌（Phytophthora parasitica var.nicotianae）、核盘病菌（Sclerotinia sclerotiorum）、玉米大斑病菌（Setosphaeria turcica）、猕猴桃软腐病菌（Botryosphaeria dothidea）的相对抑制率均达100%,且所列稀释液与发酵原液间无显著差异。牛津杯法结果表明,上清液B中不含抑菌活性物质,而上清液A与胞外代谢物粗提液中均含有抑菌活性物质。显微镜观察结果显示,胞外代谢物粗提液中的抑菌活性物质可使病原菌菌丝消融、断裂、扭曲、缠绕变形、原生质体凝集渗漏、形成泡囊结构等。研究表明,菌株F11病原菌的抑菌效果随发酵液稀释倍数增加而下降,其对樟树炭疽病菌（Colletotrichum gloeosporioides）的防治效果最好,发酵液经1 000倍稀释后相对抑制率仍达100%;对烟草黑胫病菌、核盘病菌、玉米大斑病菌、猕猴桃软腐病菌四种病原菌则可采用10倍稀释液代替发酵原液进行防治。其抑菌活性物质存在于胞外代谢物粗提液中,该物质的抑菌特性和脂肽抗生素的作用基本一致,推测为一类脂肽抗生素。     

甘肃玉米大斑病菌对吡唑醚菌酯的敏感性监测分析

为明确甘肃玉米大斑病菌对吡唑醚菌酯的敏感基线和抗药性水平,采用平皿法测定甘肃省4个典型生态区57株玉米大斑病菌对吡唑醚菌酯的敏感性。结果表明,甘肃玉米大斑病菌对吡唑醚菌酯的敏感性差异较小,EC50为0.10~1.56mg/L,EC50平均值为0.66mg/L,49株菌株EC50连续性正态分布时的EC50平均值0.61mg/L即为甘肃玉米大斑病菌对吡唑醚菌酯的敏感基线;基于此发现,甘肃玉米大斑病菌对吡唑醚菌酯的平均抗性为1.08,最高抗性为2.55,暂未出现抗药性,其中中部干旱雨养生态区5株菌株十分敏感,平均抗性和最高抗性分别为0.84和1.11,而河西干旱灌溉生态区15株菌株敏感性较低,平均抗性和最高抗性分别为1.23和2.55,需警惕其抗药性。     

Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea

MgCdc42 （Cdc42 in Magnaporthe grisea）, with high homology to ScCdc42 （Cdc42 in Saccharomyces cerevisiae）, has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network, the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBeml, Chin1, and MgGicl in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRgal, and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.     

产Iturins族脂肽生防芽孢杆菌的分离、筛选及鉴定

为获得能够产Iturins类脂肽的芽孢杆菌以应用于植物的生物防治中,以果蔬体内、根际土壤等多生境中分离的118株细菌为出发菌株,采用平板对峙法、管碟法对其进行了初筛和复筛。结果表明:其中3株菌株对立枯丝核菌、瓜果腐霉和尖孢镰刀菌显示出强烈的抑制作用;通过薄层层析等方法对产Iturins脂肽菌株进行初步分析,最终确定K103为目标菌株。通过形态学鉴定、生理生化试验及16SrDNA的分子生物学等方法,对产抗菌脂肽的K103菌株进行分类学鉴定,最终确定菌株K103为解淀粉芽孢杆菌,并将其命名为解淀粉芽孢杆菌K103(Bacillus amyloliquefaciens K103)。     

Isolation and identification of the antagonistic strain DM-54 of Bacillus amyloliquefacien against Verticillium dahliae, and optimization of antifungal protein producing conditions

The strains capable of resistance against Verticillium dahliae Kleb were isolated and screened from the soils of cotton fields from several different provinces in China. A strain, coded DM-54, with a rather high antagonistic activity was obtained. Its morphological characteristics, physiological and biochemical properties and a 16 S rDNA sequence of this strain were further studied. The DM-54 strain was finally identified as a kind of Bacillus amyloliquefacien. Through a single factor experiment and an orthogonal experiment, the optimal shaking flask fermentation condition of strain DM-54 was found to be: media composed of 5% dextrin, 3% soy peptone, 0.02% MgSO₄, 0.01% CaCl₂, initial pH 7.0 and 10% inoculum volume, media volume 30/250 (mL/mL), fermentation temperature at 32°C, rotating speed 200 r·min⁻¹, fermentation time of 48 h. Its antagonistic activity was distinguished to be elevated, at about 39.9%. Our research offers an effective means for the massive production of antagonistic proteins.     

Distribution of mating types and genetic diversity induced by sexual recombination in Setosphaeria turcica in northern China

Mature ascocarps and ascospores in the heterothallic ascomycete fungus, Setosphaeria turcica, were successfully produced in Sach’s medium with barley culm as the mating stimulator after four weeks’ coincubation of two opposite mating type isolates at 25°C in darkness. A single isolate could not produce ascospores or ascocarps. The ascocarps were produced on the exposed surface and embedded parts of barley culm or in the upper layer of the medium. The asci linked themselves to ascocarp with their short handles and assembled at the bottom of the ascocarp. Many asci had four to six colorless mature ascospores with one to six septa. But asci with eight ascospores were also found. Using isolate 9914 and isolate 9961 as standard testers for mating types (MAT1 and MAT2), respectively, 94 isolates of S. turcica collected from northern China in 1999, 2003, and 2004 were grouped into three mating types: MAT1 (53 isolates), MAT2 (31 isolates) and MAT12 (10 isolates). The MAT12 isolates, which were first found in China, were compatible with not only MAT1 isolates but also MAT2 isolates. No MAT12 isolates were found in 1999, but 2 MAT12 isolates and 8 MAT12 isolates were found in 2001 and 2003, respectively. The geographic distribution of different mating types was unequal among locations. Generally the frequency of MAT1 was significantly higher than that of MAT2 and MAT12. The unequal distribution of mating types suggested a low frequency of genetic recombination. The pathogenicity of different mating type isolates was tested on the susceptible corn inbred B37 and the results revealed that the disease latency period, disease incidence, lesion area and conidia production were not significantly different among the three mating type groups. However, the pathogenicity of the progeny isolates of isolate 99-12 (MAT2, race 1) and isolate 99-15 (MAT1, race 0) was significantly different from the parent isolates, isolate 99-12 and isolate 99-15, suggesting that sexual recombination could cause significantly virulence variation in S. turcica. Random amplification of polymorphic DNA (RAPD) analysis also revealed high genotype diversity among the progeny isolates, indicating that the sexual recombination could also produce significant genetic variation in the fungal pathogen.     

Fatty Acids and Antimicrobial Properties of Red Table Wine

The article presents research findings on fatty acids and antibacterial properties of red wine, which is known for its unique physiological effect on the human body. The subjects of research were red table wines (the vintage of 2016) produced at the Derbent sparkling wine factory from grapes grown in Derbentskii district of the Dagestan Republic. Wines were produced from a Cabernet grape variety from the Saccharomyces cerevisiae Y-4270 (experiment) and Saccharomyces cerevisiae Derbent-19 (control) wine yeast strains. Fatty acid compositions using gas-liquid chromatography and the antibacterial properties against gram-positive bacteria with a standard agar diffusion technique were examined. A total of 28 fatty acids (C₁₀–C₂₂) were identified in all samples of wine. The experimental sample was noted for a reduced content (by 8.63%) of saturated fatty acids undesirable for living organisms as the factor of nutrition that increases levels of cholesterol and atherogenic lipoprotein. The total amount of unsaturated fatty acids was observed to increase by 18.67% due to the dominance of С_18:2, С_18:2ω-6, and С_24:1ω-9. The predominance of polyunsaturated fatty acids (double), monounsaturated (by 9.56%), and polyenoic fatty acids (by 12.09%), which contribute to the composition of wine flavor, was detected. The amount of ω-6 acids is 42.12% higher, primarily owing to linoleic C_18:2ω-6 (by 16.48%) and γ-Linolenic C_18:3ω-6 (almost by a factor of four) acids. A significant content of ω-3 acids (almost by a factor of 11) was revealed, specifically, linolenic C_18:3ω-3, eicosapentaenoic C_20:5ω-3, and docosahexaenoic C_22:6ω-3 acids, which improve lipid metabolism and have a positive effect on the immune system. In general, the total content of ω-9 acids was sufficiently high in both samples of wine due to the predominance of oleic C_18:1ω-9 acid. The experimental red table wine has been found to possess antibacterial properties against bacteria Shigella sonnei, Salmonella typhimurium, Klebsiella pneumoniae, Escherichia coli, Proteus vulgaris, Proteus mirabilis, and Staphylococcus saprophyticus, resulting from the constituent conditions of the wine, including polyphenolic and antioxidant compounds, which provide the product with improved biochemical and nutrient properties.     

玉米大斑病菌STK1原核表达载体的构建及其表达

【目的】构建玉米大斑病菌STK1原核表达载体并进行表达,以期得到带有His标签的目的蛋白。【方法】根据GenBank中STK1(AY849317)的cDNA序列及原核表达载体pET28a(+)中的多克隆位点设计引物,进行STK1的克隆和原核表达载体的构建。将重组载体在原核表达体系中进行表达。通过SDS-PAGE电泳鉴定蛋白的表达,并利用Western blot技术验证该蛋白是否为目的蛋白。【结果】STK1在大肠杆菌中的表达主要以包涵体形式存在;蛋白的分子量约为40.8kD;经1mmol·L-1 IPTG在37℃下诱导,9h后蛋白产量达到最高;经Western blot检测该表达产物具有His-6抗原性。【结论】玉米大斑病菌MAPK信号转导途径中的STK1在大肠杆菌中获得了表达,为今后STK1蛋白的多克隆抗体制备及功能研究奠定基础。     

一株高效好氧反硝化细菌的分离与鉴定

采用选择性培养基通过定量增加亚硝酸盐含量来富集筛选好氧反硝化细菌,经过选择性培养基初步筛选,测定NO-2-N与NO-3-N的去除率,再通过反硝化培养基复筛选出同时具有去除NO-2-N与NO-3-N能力的目的菌。通过16S rRNA基因序列分析及同源性比对以及与其他已筛选出的部分硝化细菌与反硝化细菌的比对构建系统发育树,结合菌株的生理生化鉴定试验,鉴定出目的菌株。在好氧、28℃培养条件下,在反硝化培养基中,该菌株5 d内将NO-2-N由3 570 mg/L降至22 mg/L,去除率达99.4%,将与NO-3-N由2 464 mg/L降至27 mg/L,去除率达98.9%。通过形态学、生理生化反应以及16S rRNA序列测定鉴定菌株DB-6为阴沟肠杆菌(Enterobacter cloacae),命名为DB-6。新筛选的阴沟肠杆菌反硝化能力较强,具有生物脱氮的应用潜质,有望应用于海水养殖水质净化。     

苜蓿根腐病的病原分离、鉴定与杀菌剂毒力测定

为了解河北苜蓿根腐病病原种类,以便有目的地加以防治,对从河北采集的苜蓿根腐病样品进行了病原分离、鉴定以及致病性和室内毒力测定。根据形态学和核糖体内转录间隔区ITS4和ITS5、延伸因子EF-1H和EF-2T序列作为引物鉴定,分离出的71株致病菌株均为镰孢菌属(Fusarium),其中,木贼镰孢(F.equiseti)55株,尖孢镰孢(F.oxysporum)7株,层出镰孢(F.proliferatum)6株,变红镰孢(F.incarnatum)2株,茄镰孢(F.solani)1株。尖孢镰孢菌株D19-2、层出镰孢菌株S45和茄镰孢菌株Q1的致病性最强。通过含毒介质法测定,结果表明,40%腈菌唑可湿性粉剂(WP)、50%多菌灵WP、嘧环·咯菌腈(37%嘧菌环胺和25%咯菌腈)水分散粒剂(WDG)、50%福美双WP、70%甲基硫菌灵WP和10%苯醚甲环唑WDG对这3个菌株菌丝生长均有较好抑制作用,而80%代森锰锌WP、99%恶霉灵WP、40%菌核净WP的抑制作用较弱。