Influence of Betaine on Pasture,Finishing, and Carcass Performance in Steers

Two experiments were conducted to investigate the effects of supplemental betaine on steer performance, fat deposition, and carcass characteristics. In Exp. 1 (grazing phase), 80 steers (317 kg) were fed either 1.0 kg of a control supplement (30% CP) or 1.0 kg of the control supplement plus 20 g of betaine per head per day. Betaine supplementation had no effect on overall gain or fat deposition (P>0.10) but increased ADG (P<0.10) during d 46 to 90 (0.64 vs 0.72 kg; control vs betaine, respectively). The pasture groups remained intact and were moved to the feedlot for 141 d and fed a control diet or the control diet plus 20 g of betaine per head per day. Betaine had no overall effect on finishing gain (P>0.10); however, this response was variable by period. Betaine increased final fat thickness and fat thickness change (P<0.10), but did not affect marbling or longissimus area (P>0.10). Supplemental betaine on pasture increased feed intake, final BW, and hot carcass weight (P<0.05) and increased final fat thickness and fat thickness change (P<0.10) during the finishing phase. In Exp. 2, supplemental betaine (40 g/d) was fed to steers (averaging 584 kg) in 11 replications during the last week before harvest. The overall weighted average increase in dressing percentage when betaine was fed was +0.34% (P<0.05). There was no effect (P>0.10) on fat thickness or marbling. Season of year did not affect response.     

Growth Performance and Serum Prolactin Concentrations of Stocker Steers Implanted with Trenbolone Acetate While Grazing Endophyte-Infected Fescue in the Spring

A 64-d grazing study was conducted with a 2 × 2 factorial arrangement of treatments to evaluate the impact of implant treatment on growth performance, hair score, and serum prolactin levels of steers grazing tall fescue with high (HE) or low (LE) levels of infection with the endophytic fungus Neotyphodium coenophialum. Mixed-breed steers (n = 130; 246 ± 3.5 kg initial BW) were allocated randomly to one of three HE or one of four LE pastures beginning April 13. Within each replication, one-half of the steers were implanted (IMP) with trenbolone acetate (40 mg) and estradiol (8 mg), and one-half were not implanted (NI). No implant treatment × endophyte level interactions were detected (P>0.10). Steers grazing HE had lower (P<0.01) BW gain, inferior (P<0.05) hair scores, and lower (P<0.01) serum prolactin concentrations on d 64 than those steers grazing LE. Total BW gains were greater (P=0.01) from IMP steers than from NI steers, but serum prolactin concentrations and hair scores did not differ (P>0.10) between IMP and NI steers on either d 36 or 64. When tabulated across forage and implant treatments, correlations were negative between overall BW gains and hair scores measured on d 64 (r = 0.28; P<0.01), and positive between overall BW gains and serum prolactin levels measured on d 36 and 64 (r = 0.33 and 0.43, respectively; P<0.001). Therefore, fescue toxicity symptoms were manifested in HE steers, and implanting trenbolene acetate and estradiol improved grazing BW gain, but no endophyte level × implant interactions were detected.     

Estimation of Protein Requirements of Feedlot Steers

Feedlot performance, carcass characteristics, and diet composition data were collected from a survey of finishing steer experiments (40 experiments; 347 kg average initial weight; data excluded Holstein steers). Data were analyzed by weighted (observations/mean) analyses of variance to determine effects of protein intake and implanting strategy on feedlot performance and carcass characteristics. Implanting strategies were defined according to prevalent or last implant type used: no implant (None); medium-potency implants (Medium): zeranol 72 mg per dose, steroid-based implants (Synovex-S or Compudose) or trenbolone acetate (TBA) alone; high-potency implants (High): TBA in combination with either steroids or zeranol. Regression procedures were utilized to estimate CP and DIP, or MP requirements. Implant effects were independent (P>0.60) of dietary protein effects and included faster (P<0.05) gains at higher intakes (P<0.05) that resulted in improved (P<0.05) feed efficiencies. Steers responded to higher dietary CP (13.3 vs 11.4%) by increasing intake (P<0.05) which resulted in faster (P<0.05) and more efficient (P=0.09) gains. Compared to nonimplanted steers, implanted steers had heavier (P<0.05) carcasses with larger (P<0.05) ribeyes and lower (P<0.05) marbling scores. Nonimplanted steers fed 13.3% CP diets had heavier (P<0.05) carcasses than nonimplanted steers fed 11.4% CP diets. Maintenance MP requirements of nonimplanted steers were greater than those of implanted steers and similar to established MP requirements. Diets of steers implanted with high-potency implants must be supplemented to contain more than 7.5 g MP/kg BW^0.75/d, especially at heavy (>450 kg) initial BW, to maximize implant response. Implanted steers have a greater ability to respond to increased dietary protein because of reduced protein requirements for maintenance.     

The Immune Response and Performance of Calves Supplemented with Zinc from an Organic and an Inorganic Source

Two experiments were conducted to determine the effects of supplemental zinc (Zn) from an organic and an inorganic source on growth performance, serum Zn concentrations, and immune response of beef calves. Treatments consisted of: i) control (no supplemental Zn), ii) Zn sulfate, or iii) Zn–amino acid complex. Zinc sources were supplemented to provide 360 mg of Zn/d. Experiment 1 was a 28-d study using 84 steers (240 ± 1.5 kg) fed bermudagrass hay (21 mg Zn/kg DM) with 1.8 kg/d of the appropriate corn-based supplement. In Exp. 2, 75 heifers (176 ± 2.5 kg) were fed bermudagrass hay (38 mg Zn/kg DM) and the supplements for 140 d. In Exp. 1, ADG was greater (P<0.05) from d 15 to 28 in calves fed supplemental Zn-amino acid compared with those fed Zn sulfate, but ADG did not differ (P>0.10) among treatments for the entire 28-d study. In Exp. 2, there was no effect (P>0.10) on ADG as a result of Zn supplementation. In Exp. 2, Zn-supplemented heifers had a greater response (P=0.06) to phytohemagglutinin 24 h after an intradermal injection. In Exp. 2, calves supplemented with Zn–amino acid complex had a greater antibody response to a second vaccination for bovine respiratory syncytial virus than did control or Zn sulfate-supplemented calves (treatment by day interaction, P=0.06). There was not a consistent benefit of supplemental Zn on growth of calves, but there was a positive impact of supplemental Zn on some immune-response measurements.     

Influence of Feed Intake Fluctuation,Feeding Frequency,Time of Feeding,and Rate of Gain on Performance by Limit-Fed Steers

Four experiments were conducted to determine the effects of feed intake fluctuation, feeding frequency, time of feeding, and rate of gain on performance by limit-fed steers. Mean initial BW for steers in Experiments 1, 2, 3, and 4 were 378 ± 43, 225 ± 19, 227 ± 20, and 249 ± 17 kg, respectively. All experiments were complete random designs, and pen was the experimental unit. In Experiment 1, 10% daily variation in feed intake resulted in decreased (P<0.10) ADG and ratio of gain to feed (G/F) compared with steers fed either a constant amount or a 10% weekly variation in feed intake. In Experiment 2, steers fed once daily at 0800 h; once daily at 1700 h; twice daily at 0800 and 1700 h; or thrice daily at 0800, 1230, and 1700 h did not affect (P>0.10) ADG or G/F. Average daily gain and G/F by steers programed to gain 1.25 kg/d were not affected (P>0.10) by 10% fluctuation in feed intake or twice daily feeding in Experiment 3. Treatments used in Experiment 4 consisted of 1) steers fed to gain 0.9 kg/d, 2) steers fed to gain 0.9 kg/d with 10% daily feed intake fluctuation, 3) steers fed to gain 1.25 kg/d, and 4) steers fed to gain 1.25 kg/d with 10% daily feed intake fluctuation. Fluctuation in feed intake did not affect (P>0.10) ADG at either rate of gain. Results suggest that feed intake fluctuation in limit-fed cattle might decrease performance early in the feeding period; however, cattle seem to adapt to fluctuating feed intake as the feeding period progresses. Time and frequency of feeding did not affect performance by limit-fed steers.     

Growth Performance and Serum Mineral Concentrations of Stocker Calves Grazing Wheat Pastures and Fed Different Sources of Magnesium,

Magnesium supplementation has been shown to benefit feedlot cattle and is typically added at high concentrations to mineral supplements for calves grazing wheat pasture. Two experiments were conducted to determine the effects of Mg-mica supplementation on performance and serum mineral concentrations of stocker calves grazing wheat pasture. Supplemental Mg was provided at 6.1 g/d of supplemental Mg/ animal, Monday through Friday. In Exp. 1, 36 mixed-breed stocker calves (243 ± 3.9 kg BW) were stratified by BW and gender and allocated randomly to one of nine groups of four animals for a 112-d grazing study. Weight gain, shrink percentage, and serum Mg, Ca, K, Cu, and Zn concentrations did not differ (P>0.10) among calves fed either weathered Mg-mica (WMM), unweathered Mg-mica (UMM), or MgO. All serum mineral concentrations were within normal physiological ranges. In Exp. 2, 64 mixed-breed stocker steers (275 ± 1.7 kg BW) were stratified by BW and allocated randomly to one of 16 groups of four animals. Weight gain during a 50-d grazing study and subsequent feedlot period did not differ (P>0.10) among calves fed either no added Mg source or WMM, UMM, or MgO. Serum Cu was higher (P<0.05), and serum Mg was lower (P<0.05), from steers fed MgO, but all serum mineral concentrations were within normal physiological ranges. Although Mg-mica is relatively high in Fe (4%), using it as a supplemental Mg source appeared to have no negative impact on growth performance or serum mineral concentrations of calves grazing wheat pasture.     

Thiamin Status and Growth Performance of Cattle New to the Feedlot Environment as Affected by Amprolium and Supplemental Dietary Thiamin

Mixed breed steers (n = 96; 211 ± 1.48 kg) were used for a 35-d trial to determine the effects of amprolium and supplemental thiamin on thiamin status and growth performance of stressed cattle. Amprolium at 0 or 5 mg/kg initial BW and thiamin at 0 or 140 mg/kg DM were fed in a 2 × 2 factorial arrangement of treatments. Amprolium was top-dressed on the daily feed for the first 21 d of the study; each steer had 50-cm bunk space available. Steers were kept in 3.7 m × 30.5 m drylots (n = 16; 6 steers per pen and 4 pens per treatment) and were fed a corn, soybean meal, cottonseed hull-based total mixed ration. A pen was used as the experimental unit. Steers fed amprolium had increased ADG from d 0 to 7 (P<0.01). Supplemental thiamin tended to increase ADG from d 0 to 21 (P<0.10). Blood thiamin monophosphate (TMP) concentrations were affected by a sampling day × supplemental thiamin interaction (P<0.001). Blood TMP concentrations were increased by supplemental thiamin on d 7, 14, 21, and 28 (P<0.001) and tended to be increased on d 35 (P=0.08). A sampling day × amprolium interaction was detected for TMP (P<0.05) and blood thiamin pyrophosphate (TPP; P<0.05) concentrations. Blood TMP and TPP concentrations were decreased on d 14, 21, and 28 (P<0.05) in the steers fed amprolium, but amprolium did not affect (P>0.10) TMP and TPP concentrations on d 7 and 35. Thiamin supplementation had no effect on the number of coccidial oocysts in feces, but steers fed amprolium had reduced numbers of coccidial oocysts (P<0.05). Supplemental thiamin and amprolium did not improve overall ADG, ADFI, or gain to feed for the 35-d trial.     

Early aspects of locoweed toxicosis and evaluation of a mineral supplement or clinoptilolite as dietary treatments.

Sixteen crossbred beef heifers were used to determine the efficacy of serum clinical profiles as diagnostic tools for detection of early stages of locoweed toxicity and to test the ability of two mineral supplements for prevention or therapy of toxicosis. Dietary treatments were (DM basis) 1) 100% sorghum sudangrass hay, 2) 80% sorghum sudangrass hay:20% locoweed, 3) 80% sorghum sudangrass hay:20% locoweed plus 100 g of Silent Herder Mineral Mix (a mineral supplement reported to alleviate locoweed toxicity), and 4) 80% sorghum sudangrass hay:20% locoweed plus 100 g of clinoptilolite (a natural zeolite clay). Diets were fed at 1.5% of BW for 28 d, after which heifers had ad libitum access to sorghum sudangrass hay for 14 d. Jugular blood was sampled before feeding every 7 d, and at 2, 4, 6, and 8 h after feeding on d 28. Compared with controls, heifers fed locoweed had elevated (P less than .01) serum alkaline phosphatase activities from d 7 through 35. On d 42, alkaline phosphatase activities in heifers previously fed locoweed were lower (P less than .05) than in control heifers. Serum glutamic oxaloacetic transaminase activities were elevated (P less than .01) in heifers fed locoweed from d 7 through 42 compared with control heifers. In heifers fed locoweed, serum Fe concentrations were less (P less than .01) on d 7 through 28, but no treatment effects were noted (P greater than .10) on d 35 or 42.(ABSTRACT TRUNCATED AT 250 WORDS)     

Feedlot and Carcass Performance of Angus-, Brangus-, Gelbvieh-, and Gelbray-Sired Crossbred Steers

Feedlot and carcass traits were evaluated for steers (n = 231) sired by Angus (A), Brangus (BA), Gelbvieh (G), and Gelbray (GB) bulls (n = 29) out of first cross (F₁) Brahman-Hereford cows. Steers were produced over 4 y and were born during spring and fall calving seasons. Brahman inheritance was 25% in A- and G-sired steers, and 44% in BA- and GB-sired steers. After weaning, steers were stockered before entering the feedlot. Steers produced in 1993 and 1994 were fed in Louisiana and individually harvested at a targeted backfat thickness of 10 mm. Steers born in 1995 and 1996 were fed in Oklahoma and group harvested at an average backfat thickness of 10 mm. Data were analyzed separately by feedlot location because of significant location and sire breed x location effects. Angus-sired steers had smaller (P<0.05) longissimus areas (LMA) and higher (P<0.01) marbling scores(MS) and quality grades than G-sired steers across both locations. Tenderness was more desirable (P<0.05) for A-sired steers compared with G-sired steers when fed in Louisiana. Angus- and G-sired steers had larger (P<0.01) LMA than BA- and GB-sired steers across both locations. Tenderness was similar (P>0.10) between steers with 25% and 44% Brahman inheritance. These data suggest that more desirable carcass quality and tenderness can be achieved with the use of A sires, relative to the other sire breeds, when mated to F₁ Brahman-Hereford dams. Improved carcass cutability resulted with the use of G sires, and in steers with 25% Brahman inheritance.     

Growth Performance by Stocker Steers Fed Magnesium-Mica During a Grazing and Feedlot Period

Two experiments were conducted to determine the effects of Mg-mica supplementation on grazing and feedlot performance of stocker steers. In Exp. 1, eight groups of six steers were fed a basal diet of 80% ground grain sorghum, 15% corn silage, and 5% control protein supplement (DM basis) or a supplement containing Mg-mica (9% of supplement; 4.5 mg/kg diet DM) for 141 d. Marbling scores tended (P<0.10) to be greater, and the percentage of carcasses grading USDA Choice or higher was greater (P<0.05), from steers fed Mg-mica than from those fed the control supplement. In Exp. 2, eight groups of nine head each were offered either a control grain sorghum-based supplement or one containing 34 g/d of Mg-mica (2.7 g Mg) while grazing smooth bromegrass pastures for 112 d. Pasture groups were then placed in feedlot pens for 120 d and fed a basal diet similar to that described above. Two groups fed each pasture supplement received a control supplement, and two received a supplement containing Mg-mica (10% of supplement; 5 mg/kg diet DM). Steers fed Mg-mica during the pasture phase tended to have heavier (P=0.11) hot carcass weights, higher (P<0.05) dressing percentages, numerically (P>0.10) higher marbling scores, and a higher percentage of carcasses grading USDA Choice than steers fed the control supplement during the pasture phase. Therefore, adding Mg-mica to pasture supplements or feedlot diets appears to have no impact on grazing or feedlot performance, but may improve carcass quality.     

Influence of Both Endophyte Infestation in Fescue Pastures and Calf Genotype on Subsequent Feedlot Performance of Steers

Three experiments were conducted to determine the influence of both the concentration of endophytic fungus infestation in tall fescue pastures and calf genotype on the subsequent health and performance of steers in the feedlot. In Exp. 1 and 2, Angus steers grazed fescue pastures in Georgia containing low, moderate, or high endophyte infestations for 182 d (Exp. 1) or 78 d (Exp. 2) with 12 steers per treatment. Steers were transported 1,600 km to Texas in October (Exp. 1) and July (Exp. 2), were fed a 93% concentrate diet during the finishing period, and were harvested at an estimated backfat thickness of 12 mm. In both trials, DMI over the entire feeding period and carcass characteristics were not affected (P>0.05) by endophyte infestation. In both trials, pasture ADG decreased, and feedlot ADG and gain to feed ratio increased as the previous pasture endophyte infestation increased (P<0.05). Serum cholesterol concentrations tended (P<0.10) to decrease with increasing endophyte infestation during the first 14 d in the feedlot. In Exp. 3, Angus and Brahman × British crossbred steers grazed fescue pastures in Georgia containing low, moderate, or high endophyte in each of 2 yr. Six steers of each breed group were on each treatment each year. Steers were transported to Texas in late August of each year, were fed a 93% concentrate finishing diet, and were harvested at an estimated individual backfat thickness of 12 mm. As endophyte infestation increased, serum urea N concentrations and gain to feed ratios increased (P<0.05), whereas pasture ADG, initial BW, transit shrink, serum cholesterol concentrations, final BW, and carcass weights decreased (P<0.05) in Angus steers, but not in Brahman-cross steers. In these studies, the adverse effects of high endophyte infestations in fescue pastures appeared to carry over to the feedlot for ca. 14 d. However, steers from highly infested pastures can compensate for poor pasture performance with improved performance in the feedlot when no adverse health effects occur. Any impact of the endophyte seems to be similar in Brahman-cross and Angus steers.     

Season-Long vs Intensive-Early Stocking with Growing Cattle Grazing Bermudagrass Pasture

Our objective was to compare the performance of weaned steer calves managed with intensive-early stocking (IES; 12.4 steers per ha for 70 d) or season-long stocking (SLS; 6.2 steers per ha for 140 d) with and without supplementation (2 × 2 factorial). Beginning on May 15, 90 steers (BW = 217 ± 0.8 kg) were randomly assigned to one of 12 common bermudagrass (Cynodon dactylon [L.] Pers.) pastures (0.81 ha each) fertilized with 168 kg of nitrogen/ha. One of the following four treatments was randomly applied to three pastures: i) SLS plus no supplement, ii) SLS plus 0.45 kg/steer of ground corn daily, iii) IES plus no supplement, and iv) IES plus 0.45 kg/steer of ground corn daily. Steers on IES were lighter (P=0.01) than SLS steers on d 70. By d 140, SLS steers supplemented with corn were 33 kg heavier (P=0.02) than nonsupplemented steers. When using SLS, corn increased the BW gain 0.5 kg/kg of corn fed; however, when IES was used, there was no benefit from corn supplementation. Total BW gain/ha did not differ (P>0.17) among treatments, but SLS with corn supplementation could have the potential to produce more BW gain/ha compared to the other treatments. Grazing systems did not affect feedlot ADG (P>0.53), but IES (175 d on feed) steers did have a higher (P<0.01) feedlot total BW gain than the SLS steers (154 d on feed). Using IES positively affected (P<0.08) dressing percentage and longissimus area compared to SLS; however, these differences in carcass characteristics were probably the result of the longer feeding period.     

Enzymatically Digested Animal Protein as a Source of Protein for Weanling Pigs

Three experiments were conducted to evaluate the use of an enzymatically digested animal protein (EDAP) as a source of protein for weanling pigs. In each experiment, treatments were replicated with four (Experiments 1 and 2) or seven (Experiment 3) pens of three to five pigs each. Each experiment lasted 3 to 4 wk for the combined Phase I (1.5% Lys in Experiments 1 and 2, 1.6% Lys in Experiment 3) and Phase II (1.3% Lys) periods. In Experiments 1 (6.7 kg; 23 d of age) and 2 (6.1 kg; 22 d of age), pigs were fed one of the following Phase I diets: 1) basal (B) diet containing corn, soybean meal (SBM), whey, fish meal, and blood cells (AP-301 G; American Protein Corporation, Ames, IA); 2) B + 4% spray-dried animal plasma (SDAP); or 3) B + 2% SDAP + 2% EDAP (SDAP + EDAP). In Phase II, the dietary groups from Phase I were divided into two subsequent groups. One group received a diet containing corn, SBM, whey, fish meal, and 2% blood cells, and the second group received the same diet with 2% EDAP, resulting in six treatments for Phase II and overall periods. In Experiment 1, ADG and ADFI were increased (P<0.10) during Phase I for pigs fed SDAP + EDAP, and ADFI was increased (P<0.10) in pigs fed SDAP. In Phase II, the EDAP addition did not affect (P>0.10) ADG, ADFI, or the ratio of gain to feed. Also, Phase I diets did not affect (P>0.10) growth performance during Phase II. Overall, ADG (P<0.10) and ADFI (P<0.04) were increased (P<0.10) in pigs fed SDAP + EDAP during Phase I. In Experiment 2, ADG and the ratio of gain to feed were increased (P<0.10) in pigs fed SDAP + EDAP during Phase I. During Phase II, ADFI was increased in pigs fed SDAP + EDAP or B + SDAP (P<0.01) relative to those fed B only (P<0.003) in Phase I. Also in Phase II, the ratio of gain to feed was increased in pigs fed SDAP + EDAP (P<0.03) relative to those fed B + SDAP. Overall, ADG and the ratio of gain to feed were not affected (P>0.10) by diet, but ADFI was increased (P<0.03) in pigs fed SDAP + EDAP relative to those fed B. In Experiment 3, all pigs (5.7 kg; 17 d of age) were fed a common Phase I diet containing SDAP + EDAP. In Phase II, ADG, ADFI, and the ratio of gain to feed were not affected (P>0.10) by the addition of 2% EDAP or 2% blood cells. In summary, pigs fed SDAP + EDAP perform equally well compared with those fed B + SDAP.     

Performance of Steers Supplemented with Zinc During Grazing and Receiving at the Feedlot

A study was conducted to compare the effects of three supplemental zinc sources on beef steers grazing winter annual pasture and during receiving in a feedlot. In southern Arkansas, 84 steers were weighed (BW = 216 ± 1.5 kg), bled via jugular puncture, and randomly assigned to 12 bermudagrass pastures (0.81 ha each) that had been overseeded with wheat, rye, and annual ryegrass (7 steers per pasture; 116 d of grazing) on January 15; the steers were allowed to graze until May 11. Steers were fed 0.45 kg daily of corn-based supplement containing 103 mg zinc from ZnSO₄, zinc amino acid complex (ZnAA), or zinc polysaccharide (ZnPOL) during the entire grazing period. On May 12, steers were shipped to a research feedlot (14 h; 1,016 km; 6.9% BW shrink) in northeast New Mexico. In the feedlot, the same groups of steers were fed a receiving diet (steam-flaked milo) that contained the same zinc sources as fed during the grazing period. Dietary concentrate was increased from 75 to 85% of dietary DM weekly. Data were analyzed as a completely random design; least squares means were separated using contrast. Steer BW on d 28 and 84 of the grazing period were greater (P<0.05) for cattle fed ZnAA and ZnPOL than for steers fed ZnSO₄; however, ADG over the 116 d of grazing and ending BW did not differ (P>0.28) among zinc sources. In the feedlot, steer BW, ADG, and feed efficiency did not differ (P>0.05) among zinc sources. Serum zinc concentrations did not differ (P>0.05) among zinc sources at any point in time. We conclude that early in the grazing period, ZnAA and ZnPOL improved steer BW compared with ZnSO₄; however, no differences were noted among zinc sources later in the grazing period or during receiving in the feedlot.     

Effects of Cull Beans,Sunflower Meal,and Canola Meal as Protein Supplements to Beef Steers Consuming Grass Hay on In Situ Digestion Kinetics

Two ruminally cannulated steers were used in an in situ incubation study to test the effects of raw cull Great Northern beans (Phaseolus vulgaris), canola meal, and sunflower meal on rate and extent of digestion of forage DM, NDF, ADF, and N and to compare the DM and N digestibilities of the supplements. Steers were allowed ad libitum access to medium quality grass hay and were assigned in each of five periods to one of five supplemental treatments. The five treatments included: 1) unprocessed Great Northern beans to supply 182 g/d CP (GNB); 2) canola meal to supply 182 g/d CP (CM); 3) a mixture of Great Northern beans and sunflower to each supply 91 g/d CP (MIX); 4) sunflower meal to supply 182 g/d CP (SFM+); and 5) sunflower meal to supply 91 g/d CP (SFM−). Each period consisted of a 7-d adaptation period, 3-d intake period, and 4-d incubation period. Two bags of supplement, two bags of forage, and two blanks were incubated for 0, 4, 8, 12, 18, 24, 36, 48, 72, or 96 h. Treatment had no effect on rate, extent of digestion, or discrete lag time of forage DM, NDF, or ADF (P>0.10). Compared with the SFM+ treatment, forage N degraded at a faster rate when steers were on the GNB (P=0.06), CM (P=0.02), or SFM− (P=0.02) treatments. Forage N in the MIX treatment degraded slower than that in the GNB treatment (P=0.03). No differences (P>0.10) were detected for forage N lag time or extent of digestion. There were no differences in the rate of digestion of supplement DM or N (P>0.80), but canola meal had a greater extent of supplement DM degradation than the sunflower meal from the SFM+ treatment (P=0.13). When supplemented to medium quality hay diets, Great Northern beans, canola meal, or a mixture of Great Northern beans and sunflower meal had similar effects on in situ forage digestion kinetics. Anti-nutritional factors in the beans did not appear to alter rumen digestion of the tested forage.     

Effect of a Hydrated Sodium Calcium Aluminosilicate on Growth Performance and Carcass Traits of Pigs

Three experiments were conducted to determine the effect of a hydrated sodium calcium aluminosilicate (HSCA) on growth and carcass traits of finishing pigs. In Experiment 1, 32 barrows (initial weight = 26.7 kg) were fed one of two dietary treatments: 1) a corn-soybean meal basal (B) diet or 2) B + 0.50% HSCA. In Experiments 2 and 3, 64 barrows and gilts were used in a 2 × 2 factorial arrangement of treatments. Two percentages of HSCA (0 or 0.50%) were fed to barrows or gilts. In Experiment 1, average daily gain, feed intake, gain:feed, serum urea nitrogen (SUN) concentration, and carcass traits were not affected (P>0.10) by HSCA. Leaf fat weight was decreased (P<0.08) in pigs fed HSCA. In Experiments 2 and 3, no HSCA × gender interactions (P>0.10) were present; thus, barrow and gilt data were combined. In Experiment 2, average daily gain, feed intake, gain:feed, SUN, and carcass traits were not affected (P>0.10) by HSCA. In Experiment 3, average daily gain, feed intake, and gain:feed were not affected (P>0.10) by HSCA. Serum urea nitrogen was increased (P<0.05) in pigs fed HSCA. Carcass traits were not affected (P>0.10) by HSCA, with the exception of increased (P<0.10) total carcass fat (total body electrical conductivity) in pigs fed HSCA. Pork quality score for color was increased (P<0.09) in pigs fed HSCA, but scores for marbling and firmness-wetness were not affected (P>0.10). In summary, HSCA did not affect growth performance or consistently affect carcass traits.     

Effects of AGRADO® on Growth Performance and Health of Newly Received Feedlot Calves

Two experiments were conducted to determine the effect of an antioxidant (AGRADO®; Solutia Inc., St. Louis, MO) on the growth, gain to feed ratio, and health of receiving calves. In Experiment 1, 96 mixed breed heifer calves (206 ± 1.4 kg initial BW) were purchased at sale barns and delivered as a single group to the research facility near Fayetteville. All heifers were fed a total mixed diet containing 30% cottonseed hulls, 53% cracked corn, and 11% soybean meal for 42 d with either 0 or 150 mg AGRADO®/kg diet. Fewer (P<0.05) of the heifers fed supplemental AGRADO® were treated for respiratory disease (73% vs 83%), and medication cost was lower (P<0.05) for heifers fed supplemental AGRADO ($6.33 vs $9.49 per pen). Supplemental AGRADO® did not affect (P>0.10) ADG, daily feed intake, and gain to feed ratio. In Experiment 2, 86 bull and steer calves (237 ± 2.2 kg initial BW) were managed and fed as in Experiment 1 for a 41-d study. In this trial, supplemental AGRADO® had no significant effect on the number of sick calves or medication costs, but gain to feed ratio was improved (P<0.05) during the first 28 d of the trial. These results indicate that supplemental AGRADO® may improve the health or growth performance of newly received feedlot cattle.     

Effects of flax supplementation and a combined trenbolone acetate and estradiol implant on circulating insulin-like growth factor-I and muscle insulin-like growth factor-I messenger RNA levels in beef cattle

We evaluated effects of a 5% (dry matter basis) ground flaxseed supplement (flax) and a trenbolone acetate and estradiol-17beta implant, Revalor-S, on circulating IGF-I and muscle IGF-I messenger RNA (mRNA). Sixteen crossbred yearling steers (initial BW = 397 kg) were assigned randomly to one of four treatments: 1) flax/implant; 2) nonflax/implant; 3) flax/nonimplant; and 4) nonflax/nonimplant. Serum was harvested from blood collected on d 0 (before implant or flax addition), 14, and 28, and used in subsequent analyses of circulating IGF-I. Biopsy samples (0.5 g) were obtained from the longissimus muscle on d 0, 14, and 28. Total RNA was isolated from the muscle samples, and real-time quantitative-PCR was used to assess relative differences in IGF-I mRNA. Flax supplementation had no effect (P > 0.10) on circulating IGF-I concentrations. Following implantation, sera from implanted steers had 52 and 84% greater (P < 0.05) IGF-I concentrations than sera from nonimplanted steers on d 14 and 28, respectively. On d 28, local muscle IGF-I mRNA levels increased 2.4-fold (P < 0.01) in biopsy samples obtained from implanted compared with nonimplanted steers. Muscle biopsy samples from nonflax cattle had 4.4-fold higher (P < 0.01) levels of IGF-I mRNA than those from flax cattle on d 28. To determine whether a component of flax, alpha-linolenic acid (alphaLA), was directly responsible for IGF-I mRNA down-regulation, we incubated primary cultures of bovine satellite cells, from implanted and nonimplanted steers, in two concentrations of alphaLA (10 nM and 1 microM). An implant x dose interaction (P < 0.05) was observed for IGF-I mRNA concentrations in bovine satellite cells cultured for 72 h with alphaLA. Satellite cells from nonimplanted steers had similar (P > 0.10) IGF-I mRNA concentration regardless of the level of alphaLA exposure; however, satellite cells from implanted steers exposed to 10 nM and 1 microM alphaLA had 2.5- and 2.0-fold greater IGF-I mRNA levels, respectively, than cells from implanted steers that were not exposed to alphaLA (P < 0.05). Administration of a Revalor-S implant increased circulating IGF-I and local muscle IGF-I mRNA concentrations in finishing cattle. However, muscle IGF-I mRNA levels were decreased by flax supplementation. Muscle cell culture experiments suggested that alphaLA was not responsible for the IGF-I mRNA down-regulation.     

Effects of Increasing Dietary Niacin on Weanling Pig Performance

Two experiments using 415 weanling pigs (4.8 ± 0.98 kg and 14 ± 4 d of age) were conducted to determine the effect of increasing dietary niacin on pig performance. Pigs were blocked by BW and randomly allotted to one of five dietary treatments. There were five pigs per pen with seven pens per treatment in Exp. 1, and eight pigs per pen with six pens per treatment in Exp. 2. Diets were fed in four phases (d 0 to 4, 4 to 8, 8 to 22, and 22 to 35). Pigs were fed the control diet with no added niacin or the control diet with 28, 55, 83, or 110 mg/kg of added niacin; data from both trials were combined. From d 0 to 22, increasing niacin had no effect (P>0.10) on growth performance. From d 22 to 35, increasing niacin had no effect (P>0.10) on ADG or ADFI, but improved (linear, P<0.04) gain:feed ratio (G:F). Overall (d 0 to 35), increasing niacin had no affect (P>0.10) on ADG or ADFI, but tended to numerically (linear, P<0.10) improve G:F. In summary, diets high in dried whey and other specialty protein sources appear to contain adequate niacin to maximize growth performance for the first 3 wk after weaning. However, in the late nursery phase (d 22 to 35) when pigs are fed corn-soybean meal diets, up to 110 mg/kg of added niacin linearly (P<0.04) improves G:F.     

Comparison of Swine Diets Containing a Food Waste Product Made with Wheat Middlings and Corn or a Corn/Soybean Diet,

Although pigs will readily consume wet food waste (FW), the high moisture content contributes to spoilage and feeding management problems. The use of a dry, processed FW product was compared with a traditional corn and soybean (CS) diet using growing swine in two performance trials and one digestibility trial. The FW diet contained approximately 20% processed FW (DM basis). In Trial 1, 24 gilts (76.4 kg) housed in eight replicated pens (four pens per treatment) were fed in a 6-wk trial. Intake, BW gain, feed efficiency, and carcass characteristics were compared. Feed intake and BW gain averaged 3.4 and 3.6 kg of DM/d and 0.87 and 0.85 kg/d for gilts fed traditional and FW diets, respectively. There were no differences in these or any other measurements (P>0.05). In Trial 2, 12 barrows (84.3 kg) housed in four replicated pens (two pens per treatment) were fed in a 6-wk trial. Intake, BW gain, feed efficiency, and carcass characteristics were compared. Feed intake and BW gain averaged 3.1 and 3.3 kg of DM/d and 0.62 and 0.71 kg/d for barrows fed traditional and FW diets, respectively. Four growing gilts (68.2 kg) were used to compare digestibility in a crossover design. There were no differences (P>0.10) for DM, CP, ADF, or NDF digestibility when feed intake averaged 1.9 kg of DM/d for both FW and CS diets. The use of up to 20% processed FW may be suitable in commercial swine diets.